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| 1 | Comprehensive analysis of NAC transcription factors in diploid Gossypium: sequence conservation and expression analysis uncover their roles during fiber development显示文摘Determining how function evolves following gene duplication is necessary for understanding gene expansion.Transcription factors(TFs)are a class of proteins that regulate gene expression by binding to specific cis-acting elements in the promoters of target genes,subsequently activating or repressing their transcription.In the present study,we systematically examined the functional diversification of the NAC transcription factor(NAC-TFs)family by analyzing their chromosomal location,structure,phylogeny,and expression pattern in Gossypium raimondii(Gr)and G.arboreum(Ga).The 145 and 141 NAC genes identified in the Gr and Ga genomes,respectively,were annotated and divided into 18 subfamilies,which showed distinct divergence in gene structure and expression patterns during fiber development.In addition,when the functional parameters were examined,clear divergence was observed within tandem clusters,which suggested that subfunctionalization had occurred among duplicate genes.The expression patterns of homologous gene pairs also changed,suggestive of the diversification of gene function during the evolution of diploid cotton.These findings provide insights into the mechanisms underlying the functional differentiation of duplicated NAC-TFs genes in two diploid cotton species. | Haihong Shang Zhongna Wang Changsong Zou Zhen Zhang Weijie Li Junwen Li Yuzhen Shi Wankui Gong Tingting Chen Aiying Liu Juwu Gong Qun Ge Youlu Yuan | 2016 | Science China(Life Sciences)2016，59，2： | 12 |
| 2 | Genome-wide Dissection of Co-selected UV-B Responsive Pathways in the UV-B Adaptation of Qingke显示文摘Qingke (Tibetan hulless barley) has long been cultivated and exposed to long-term and strong UV-B radiation on the Tibetan Plateau, which renders it an ideal species for elucidating novel UV-B responsive mechanisms in plants. Here we report a comprehensive metabolite profiling and metabolite-based genome-wide association study (mGWAS) using 196 diverse qingke and barley accessions. Our results demonstrated both constitutive and induced accumulation, and common genetic regulation, of metabolites from different branches of the phenylpropanoid pathway that are involved in UV-B protection. A total of 90 significant mGWAS loci for these metabolites were identified in barley-qingke differentiation regions, and a number of high-level metabolite trait alleles were found to be significantly enriched in qingke, suggesting co-selection of various phenylpropanoids. Upon dissecting the entire phenylpropanoid pathway, we identified some key determinants controlling natural variation of phenylpropanoid content, including three novel proteins, a flavone C-pentosyltransferase, a tyramine hydroxycinnamoyl acyltransferase, and a MYB transcription factor. Our study, furthermore, demonstrated co-selection of both constitutive and induced phenylpropanoids for UV-B protection in qingke. | Xingquan Zeng Hongjun Yuan Xuekui Dong Meng Peng Xinyu Jing Qijun Xu Tang Tang Yulin Wang Sang Zha Meng Gao Congzhi Li Chujin Shu Zexiu Wei Wangmu Qimei Yuzhen Basang Jiabu Dunzhu Zeqing Li Lijun Bai Jian Shi Zhigang Zheng Sibin Yu Alisdair R.Fernie Jie Luo Tashi Nyima | 2020 | Molecular Plant2020，13，1： | 9 |
| 3 | Constructing a high-density linkage map for Gossypium hirsutum × Gossypium barbadense and identifying QTLs for lint percentage显示文摘To introgress the good fiber quality and yield from Gossypium barbadense into a commercial Upland cotton variety, a high-density simple sequence repeat(SSR) genetic linkage map was developed from a BC1F1 population of Gossypium hirsutum×Gossypium barbadense. The map comprised 2,292 loci and covered 5115.16 centi Morgan(c M) of the cotton AD genome, with an average marker interval of 2.23 c M. Of the marker order for 1,577 common loci on this new map, 90.36% agrees well with the marker order on the D genome sequence genetic map. Compared with five published high-density SSR genetic maps, 53.14% of marker loci were newly discovered in this map. Twenty-six quantitative trait loci(QTLs) for lint percentage(LP) were identified on nine chromosomes. Nine stable or common QTLs could be used for marker-assisted selection. Fifty percent of the QTLs were from G. barbadense and increased LP by 1.07%–2.41%. These results indicated that the map could be used for screening chromosome substitution segments from G. barbadense in the Upland cotton background, identifying QTLs or genes from G. barbadense, and further developing the gene pyramiding effect for improving fiber yield and quality. | Yuzhen Shi Wentan Li Aiguo Li Ruihua Ge Baocai Zhang Junzhi Li Guangping Liu Junwen Li Aiying Liu Haihong Shang Juwu Gong Wankui Gong Zemao Yang Feiyü Tang Zhi Liu Weiping Zhu Jianxiong Jiang Xiaonan Yu Tao Wang Wei Wang Tingting Chen Kunbo Wang Zhengsheng Zhang Youlu Yuan | 2015 | Journal of Integrative Plant Biology2015，57，5： | 7 |
| 4 | Cu-Co bi-metal catalyst prepared by perovskite CuO/LaCoO_3 used for higher alcohol synthesis from syngas显示文摘Cu-Co bi-metal catalysts derived from CuO/LaCoO3 perovskite structure were prepared by one-step citrate complexing method, and the structure evolution reaction from CuO/LaCoO3 to Cu-Co2C/La2O2CO3 under H2pretreatment was investigated by techniques of XRD, TPR and TEM. The results suggest that a much higher dispersion of copper significantly enhanced the reduction of cobalt, and a stronger interaction between copper and cobalt ions in LaCoO3 particles led to the formation of bi-metallic Cu-Co particles in the reduced catalysts and the enrichment of Co on the surface of bimetallic particles. The prepared catalysts were highly active and selective for the alcohol synthesis from syngas due to the presence of copper-modified Co2 C species. | Yuzhen Fang Yuan Liu Wei Deng Junhai Liu | 2014 | Journal of Energy Chemistry2014，23，4： | 5 |
| 5 | Perovskite LaFeO_3 supported bi-metal catalyst for syngas methanation显示文摘LaFeO3 perovskite supported Ni and Ni-Fe catalysts were prepared and applied to methanation reaction of syngas.Two preparation methods were employed.One was one-step citrate complexing method,and the other was a two step method using citrate complexing method to produce LaFeO3 and followed by loading nickel oxide on it with impregnation.The structure evolution of the sample as prepared was investigated by XRD,TPR and TEM techniques.For the former,the chemical composites of the calcined sample are NiO-Fe2O3/LaFe1-xNix O3.After reduction and reaction of CO methanation,its composites convert to Fe-Ni@Ni/LaFeO3-La2O2CO3,in which Fe-Ni@Ni is metal particles in nano-size composed of nickel core and Fe-Ni alloy shell.For the latter,the chemical composites of the calcined sample are NiO/LaFeO3; and after reduction and reaction of CO methanation,its chemical composites change to Ni/LaFeO3.Ni/LaFeO3 catalyst is a little more active, while Fe-Ni@Ni/LaFeO3-La2O2CO3 is much more stable and shows very good resistance to carbon deposition.In this work it is aimed to show that the structure and composites of the catalysts can be tailored using perovskite-type oxide as precursor prepared with different methods and conditions.Therefore,it is a promising route to prepare supported bi-metal catalysts in nano-size for a lot of metals with desired catalytic performances. | Hong Wang Yuzhen Fang Yuan Liu Xue Bai | 2012 | Journal of Natural Gas Chemistry2012，21，6： | 5 |
| 6 | The applicability of biochar and zero-valent iron for the mitigation of arsenic and cadmium contamination in an alkaline paddy soil显示文摘In paddy fields, the opposing transformation of arsenic (As) and cadmium (Cd) poses many challenges for their simultaneous remediation.In our previous study,we reported that combined biochar and zero-valent iron(ZVI)amendment had great potential for the simultaneous alleviation of As and Cd bioavailability in contaminated acid paddy soil.In this study,an As-and Cd-contaminated alkaline paddy soil was further studied,and the same ZVI-biochar mixtures amendments were applied to evaluate the impact of the mixtures on As and Cd transformation and translocation in the soil-rice system by performing pot experiments with rice.In line with our previous study,the ZVI-biochar composites significantly reduced As and Cd accumulation in different rice tissues,leading to a 42%and 47%decrease in rice grain As and Cd levels,respectively,compared with the control values.The ZVI-biochar mixtures exhibited synergistic effects of biochar and ZVI by enhancing the transformation of bioavailable As and Cd fractions into less bioavailable fractions,and by increasing iron plaque formation to reduce As and Cd bioavailability.Although the bioaccumulation and translocation factors of As and Cd in alkaline paddy soil were generally lower than those in acid paddy soil,particularly in the presence of the ZVI-biochar mixtures,the grain As and Cd levels did not achieve the desired food safety standard levels,probably related to the high soil As content and the small changes in soil pH.Nevertheless,for treating lightly and moderately contaminated paddy soils,ZVI-biochar mixtures can still be a good choice in the future. | Jiangtao Qiao Huanyun Yu Xiangqin Wang Fangbai Li Qi Wang Yuzhen Yuan Chuanping Liu | 2019 | Biochar2019，1，2： | 5 |
| 7 | Liensinine induces gallbladder cancer apoptosis and G2/M arrest by inhibiting ZFX-induced PI3K/AKT pathway显示文摘Gallbladder carcinoma (GBC) is the most comm on and aggressive can cer of the biliary tract. Liensinine has been proved to have hypotensive effect. However, the effect of liensinine on GBC is still unknown. The aim of this study is to investigate the effect and mechanism of liensinine in human GBC cells. Cell viability assay and colony formation assay were performed to assess cell growth and proliferation. Flow cytometry analysis was used to investigate cell cycle apoptosis in vitro. Hoechst 33342 staining was also used to evaluate cell apoptosis. Western blot analysis was used to determine the expression of proteins corresponding to the related cell cycle and apoptosis.The effect of liensinine treatment in vivo was experimented with xenografted tumors. We found that liensinine significantly inhibited the growth of GBC cells both in vivo and in vitro. In vitro, cell growth and proliferation were significantly suppressed by liensinine in a dose- and time-dependent manner. In vivo, liensinine inhibited tumor growth. Liensinine could induce GBC cells G2/M phase arrest by up-regulating the levels of Cyclin B1 and CDK1 proteins. Liensinine also affected GBC cell cycle progress!on and induced apoptosis by down-regulating phosphorylated protein kinase B (AKT), phosphorylated protein kinase B (p-AKT), phosphatidylinositol 3-kinase (PI3K), and Zinc finger X-chromosomal protein (ZFX) proteins. Liensinine induced G2/M arrest and apoptosis in gallbladder cancer, suggest!ng that liensinine might represent a novel and effective agent against gallbladder cancer. | Yitong Shen Rui Bian Yaxiong Li Yuan Gao Yingbin Liu Yuzhen Xu Xiaoling Song Yijian Zhang | 2019 | Acta Biochimica et Biophysica Sinica2019，51，6： | 4 |
| 8 | Tailoring bioinks of extrusion-based bioprinting for cutaneous wound healing显示文摘Extrusion-based bioprinting (EBB) holds potential for regenerative medicine. However, the widely-used bioinks of EBB exhibit some limitations for skin regeneration, such as unsatisfactory bio-physical (i.e., mechanical, structural, biodegradable) properties and compromised cellular compatibilities, and the EBB-based bioinks with therapeutic effects targeting cutaneous wounds still remain largely undiscussed. In this review, the printability considerations for skin bioprinting were discussed. Then, current strategies for improving the physical properties of bioinks and for reinforcing bioinks in EBB approaches were introduced, respectively. Notably, we highlighted the applications and effects of current EBB-based bioinks on wound healing, wound scar formation, vasculari-zation and the regeneration of skin appendages (i.e., sweat glands and hair follicles) and discussed the challenges and future perspectives. This review aims to provide an overall view of the applications, challenges and promising solutions about the EBB-based bioinks for cutaneous wound healing and skin regeneration. | Yuzhen Wang Xingyu Yuan Bin Yao Shuoji Zhu Ping Zhu Sha Huang | 2022 | Bioactive Materials2022，7，11： | 3 |
| 9 | QTL mapping for fiber quality and yieldrelated traits across multiple generations in segregating population of CCRI 70显示文摘Background:Cotton is a significant economic crop that plays an indispensable role in many domains.Gossypium hirsutum L.is the most important fiber crop worldwide and contributes to more than 95%of global cotto n production.Identifying stable quantitative trait locus(QTLs)controlling fiber quality and yield related traits are necessary prerequisites for marker-assisted selection(MAS).Results:A genetic linkage map was constructed with 312 simple sequence repeat(SSR)loci and 35 linkage groups using JoinMap 4.0;the map spanned 1 929.9 cM,with an average interval between two markers of 6.19 cM,and covered approximately 43.37%of the cotton genome.A total of 74 QTLs controlling fiber quality and 41 QTLs controlling yield-related traits were identified in 4 segregating generations.These QTLs were distributed across 20 chromosomes and collectively explained 1.01%?27.80%of the observed phenotypic variations.In particular,35 stable QTLs could be identified in multiple generations,25 common QTLs were con sistent with those in previous studies,and 15 QTL clusters were found in 11 chromosome segments.Conclusion:These studies provide a theoretical basis for improving cotton yield and fiber quality for molecular marker-assisted selection. | DENG Xiaoying GONG Juwu LIU Aiying SHI Yuzhen GONG Wankui GE Qun LI Junwen SHANG Haihong WU Yuxiang YUAN Youlu | 2019 | Journal of Cotton Research2019，2，2： | 3 |
| 10 | Infuence of Substrate Temperature on Stress and Morphology Characteristics of Co Doped ZnO Films Prepared by Laser-Molecular Beam Epitaxy显示文摘Zn1exCox O(x 0.05) thin films are deposited on sapphire(0001) substrates by laser-molecular beam epitaxy technique at different substrate temperatures. The structural, stress and morphology evolution features are investigated by means of X-ray diffraction and atomic force microscopy. The surface parameters of roughness exponent a, root mean square(RMS) roughness w and autocorrelation length x are calculated and the surface parameters are preliminarily analyzed. The values of a vary from 0.7 to 0.9. The RMS roughness w is less than 2.2 nm, and it increases with increasing T s from 300 to 400 C, and then decreases when T s is 500 C. The autocorrelation length x decreases monotonously with the increase in T s from 300 to 500 C, which indicates that the increase in T s restrains the spread of the surface fluctuations until T s is higher than 400 C. | Yunyan Liu Shanying Yang Gongxiang Wei Jiaoqing Pan Yuzhen Yuan Chuanfu Cheng | 2013 | Journal of Materials Science & Technology2013，29，12： | 3 |
| 11 | Using bioprinting and spheroid culture to create a skin model with sweat glands and hair follicles显示文摘Background:Sweat glands(SGs)and hair follicles(HFs)are two important cutaneous appendages that play crucial roles in homeostatic maintenance and thermoregulation,and their interaction is involved in wound healing.SGs can be regenerated from mesenchymal stem cell-laden 3D bioprinted scaffolds,based on our previous studies,whereas regeneration of HFs could not be achieved in the same model.Due to the lack of an in vitro model,the underlying molecular mechanism of the interaction between SGs and HFs in regeneration could not be fully understood.The purpose of the present study was to establish an in vitro model of skin constructs with SGs and HFs and explore the interaction between these two appendages in regeneration.Methods:To investigate the interaction effects between SGs and HFs during their regeneration processes,a combined model was created by seeding HF spheroids on 3D printed SG scaffolds.The interaction between SG scaffolds and HF spheroids was detected using RNA expression and immunofluorescence staining.The effects of microenvironmental cues on SG and HF regeneration were analysed by altering seed cell types and plantar dermis homogenate in the scaffold.Results:According to this model,we overcame the difficulties in simultaneously inducing SG and HF regeneration and explored the interaction effects between SG scaffolds and HF spheroids.Surprisingly,HF spheroids promoted both SG and HF differentiation in SG scaffolds,while SG scaffolds promoted SG differentiation but had little effect on HF potency in HF spheroids.Specifically,microenvironmental factors(plantar dermis homogenate)in SG scaffolds effectively promoted SG and HF genesis in HF spheroids,no matter what the seed cell type in SG scaffolds was,and the promotion effects were persistent.Conclusions:Our approach elucidated a new model for SG and HF formation in vitro and provided an applicable platform to investigate the interaction between SGs and HFs in vitro.This platform might facilitate 3D skin constructs with multiple appendages and unveil the spatiotemporal molecular program of multiple appendage regeneration. | Yijie Zhang Enhejirigala Bin Yao Zhao Li Wei Song Jianjun Li Dongzhen Zhu Yuzhen Wang Xianlan Duan Xingyu Yuan Sha Huang Xiaobing Fu | 2021 | Burns & Trauma2021，9，1： | 2 |
| 12 | Chlorination pattern effect on thermodynamic parameters and environmental degradability for C_(10)-SCCPs: Quantum chemical calculation based on virtual combinational library显示文摘Short-chain chlorinated paraffins(SCCPs) are still controversial candidates for inclusion in the Stockholm Convention.The inherent mixture nature of SCCPs makes it rather difficult to explore their environmental behaviors.A virtual molecule library of 42,720 C_(10)-SCCP congeners covering the full structure spectrum was constructed.We explored the structural effects on the thermodynamic parameters and environmental degradability of C_(10)-SCCPs through semi-empirical quantum chemical calculations.The thermodynamic properties were acquired using the AM1 method,and frontier molecular orbital analysis was carried out to obtain the E_(HOMO),E_(LUMO) and E_(LUMO)-E_(HOMO) for degradability exploration at the same level.The influence of the chlorination degree(N_(Cl) on the relative stability and environmental degradation was elucidated.A novel structural descriptor,μ,was proposed to measure the dispersion of the chlorine atoms within a molecule.There were significant correlations between thermodynamic values and N_(Cl),while the reported N_(Cl)-dependent pollution profile of C_(10)-SCCPs in environmental samples was basically consistent with the predicted order of formation stability of C_(10)-SCCP congeners.In addition,isomers with largeμ showed higher relative stability than those with small μ.This could be further verified by the relationship between μ and the reactivity of nucleophilic substitution and · OH attack respectively.The C_(10)-SCCP congeners with less Cl substitution and lower dispersion degree are susceptible to environmental degradation via nucleophilic substitution and hydroxyl radical attack,while direct photolysis of C_(10)-SCCP congeners cannot readily occur due to the large E_(LUMO)-E_(HOMO) values.The chlorination effect and the conclusions were further checked with appropriate density functional theory(DFT) calculations. | Yuzhen Sun Wenxiao Pan Yuan Lin Jianjie Fu Aiqian Zhang | 2016 | Journal of Environmental Sciences2016，28，1： | 2 |
| 13 | An intelligent displacement back-analysis method for earth-rockfill dams显示文摘 | Yu Yuzhen zhang Bingyin Yuan Huina | 2007 | Computers and Geotechnics2007，34，6： | 1 |
| 14 | Highly sensitive and specific determination of mercury(II) ion in water, food and cosmetic samples with an ELISA based on a novel monoclonal antibody显示文摘 | Yuzhen Wang Hong Yang Michael Pschenitza Reinhard Niessner Yuan Li Dietmar Knopp Anping Deng | 2012 | Analytical and Bioanalytical Chemistry2012，，9： | 1 |
| 15 | Cotton germplasm improvement and progress in Pakistan显示文摘Cotton(Gossypium spp.)contributes significantly to the economy of cotton-producing countries.Pakistan is the fourth-largest producer of cotton after China,the USA and India.The average yield of cotton is about 570.99 kg.hm^(−2) in Pakistan.Climate change and different biotic stresses are causing reduction in cotton production.Transgenic approaches have unique advantage to tackle all these problems.However,how to confer permanent resistance in cotton against insects through genetic modification,is still a big challenge to address.Development of transgenic cotton has been proven to be effective.But its effectiveness depends upon several factors,including heterogeneity,seed purity,diffusion of varieties,backcrossing and ethical concerns.Cotton biotechnology was initiated in Pakistan in 1992–1993 with a focus on acquiring cotton leaf curl virus(CLCuV)-resistant insect-resistant,and improving fiber quality.This review summarizes the use of molecular markers,QTLs,GWAS,and gene cloning for cotton germplasm improvement,particularly in Pakistan. | RAZZAQ Abdul ZAFAR Muhammad Mubashar ALI Arfan HAFEEZ Abdul BATOOL Wajeeha SHI Yuzhen GONG Wankui YUAN Youlu | 2021 | Journal of Cotton Research2021，4，1： | 1 |
| 16 | Fingerprint Identification of Ginseng Drugs显示文摘 | Xie Peishan Yuan Yuzhen | 1987 | Journal of High Resolution Chromatography Communication1987，10，11： | 1 |
| 17 | Differentiation &Evaluation of commercial Ginseng& their products by Means of HPTLC Fingerprint Analysis显示文摘 | Xie Peishan Yuan Yuzhen | 1998 | Journal of Planal Chromatography Modern TLC1998，1，1： | 1 |
| 18 | QTL mapping for plant height and fruit branch number based on RIL population of upland cotton显示文摘Background:Plant height(PH)and fruit branch number(FBN)are important traits for improving yield and mechanical harvesting of cotton.In order to identify genes of PH and FBN in cotton germplasms to develop superior cultivars,quantitative trait loci(QTLs)for these traits were detected based on the phenotypic evaluation data in nine environments across four locations and 4 years and a previously reported genetic linkage map of an recombinant inbred line(RIL)population of upland cotton.Results:In total,53 QTLs of PH and FBN,were identified on 21 chromosomes of the cotton genome except chromosomes c02,c09-c11,and c22.For PH,27 QTLs explaining 3.81%–8.54%proportions of phenotypic variance were identified on 18 chromosomes except c02,c08-c12,c15,and c22.For FBN,26 QTLs explaining 3.23%–11.00%proportions of phenotypic variance were identified on 16 chromosomes except c02-c03,c06,c09-c11,c17,c22-c23,and c25.Eight QTLs were simultaneously identified in at least two environments.Three QTL clusters containing seven QTLs were identified on three chromosomes(c01,c18 and c21).Eleven QTLs were the same as previously reported ones,while the rest were newly identified.Conclusions:The QTLs and QTL clusters identified in the current study will be helpful to further understand the genetic mechanism of PH and FBN development of cotton and will enhance the development of excellent cultivars for mechanical managements in cotton production. | LIU Ruixian XIAO Xianghui GONG Juwu LI Junwen ZHANG Zhen LIU Aiying LU Quanwei SHANG Haihong SHI Yuzhen GE Qun IQBAL Muhammad Sajid CHEN Quanjia YUAN Youlu GONG Wankui | 2020 | Journal of Cotton Research2020，3，1： | 1 |
| 19 | An intelligent displacement back-analysis method for earthrockfill dams显示文摘 | YU YUZHEN ZHANG BINGYIN YUAN HUINA | 2007 | Computers and Geotechnics2007，34，4： | 1 |
| 20 | Fingerprint Identification of Ginseng Drugs显示文摘 | Xie Peishan Yuan Yuzhen | 1987 | Journal of High Resolution Chromatography Communication1987，10，11： | 1 |
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