|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | A two-step lineage reprogramming strategy to generate functionally competent human hepatocytes from fibroblasts显示文摘Terminally differentiated cells can be generated by lineage reprogramming,which is,however,hindered by incomplete conversion with residual initial cell identity and partial functionality. Here, we demonstrate a new reprogramming strategy by mimicking the natural regeneration route, which permits generating expandable hepatic progenitor cells and functionally competent human hepatocytes. Fibroblasts were first induced into human hepatic progenitor-like cells (hHPLCs), which could robustly expand in vitro and efficiently engraft in vivo. Moreover, hHPLCs could be efficiently induced into mature human hepatocytes (hiHeps) in vitro, whose molecular identity highly resembles primary human hepatocytes (PHHs). Most importantly, hiHeps could be generated in large quantity and were functionally competent to replace PHHs for drug-metabolism estimation, toxicity prediction and hepatitis B virus infection modeling. Our results highlight the advantages of the progenitor stage for successful lineage reprogramming. This strategy is promising for generating other mature human cell types by lineage reprogramming. | Bingqing Xie Da Sun Yuanyuan Du Jun Jia Shicheng Sun Jun Xu Yifang Liu Chengang Xiang Sitong Chen Huangfan Xie Qiming Wang Guangya Li Xuehui LYU Hui Shen Shiyu Li Min Wu Xiaonan Zhang Yue Pu Kuanhui Xiang Weifeng Lai Peng Du Zhenghong Yuan Cheng Li Yan Shi Shichun Lu Hongkui Deng | 2019 | Cell Research2019，29，9： | 9 |
| 2 | VO2·0.2H2O nanocuboids anchored onto graphene sheets as the cathode material for ultrahigh capacity aqueous zinc ion batteries显示文摘Aqueous Zinc-ion batteries(ZIBs),using zinc negative electrode and aqueous electrolyte,have attracted great attention in energy storage field due to the reliable safety and low-cost.A composite material comprised of VO2·0.2H2O nanocuboids anchored on graphene sheets(VOG)is synthesized through a facile and efficient microwave-assisted solvothermal strategy and is used as aqueous ZIBs cathode material.Owing to the synergistic effects between the high conductivity of graphene sheets and the desirable structural features of VO2·0.2H2O nanocuboids,the VOG electrode has excellent electronic and ionic transport ability,resulting in superior Zn ions storage performance.The Zn/VOG system delivers ultrahigh specific capacity of 423 mAh·g^−1 at 0.25 A·g^−1 and exhibits good cycling stability of up to 1,000 cycles at 8 A·g^−1 with 87%capacity retention.Systematical structural and elemental characterizations confirm that the interlayer space of VO2·0.2H2O nanocuboids can adapt to the reversible Zn ions insertion/extraction.The as-prepared VOG composite is a promising cathode material with remarkable electrochemical performance for low-cost and safe aqueous rechargeable ZIBs. | Dedong Jia Kun Zheng Ming Song Hua Tan Aitang Zhang Lihua Wang Lijun Yue Da Li Chenwei Li Jingquan Liu | 2020 | Nano Research2020，13，1： | 6 |
| 3 | Interferon-induced Transmembrane Protein 3 Prevents Acute Influenza Pathogenesis in Mice显示文摘Objective Interferon-induced transmembrane protein 3(IFITM3)is an important member of the IFITM family.However,the molecular mechanisms underlying its antiviral action have not been completely elucidated.Recent studies on IFITM3,particularly those focused on innate antiviral defense mechanisms,have shown that IFITM3 affects the body’s adaptive immune response.The aim of this study was to determine the contribution of IFITM3 proteins to immune control of influenza infection in vivo.Methods We performed proteomics,flow cytometry,and immunohistochemistry analysis and used bioinformatics tools to systematically compare and analyze the differences in natural killer(NK)cell numbers,their activation,and their immune function in the lungs of Ifitm3-/-and wild-type mice.Results Ifitm3-/-mice developed more severe inflammation and apoptotic responses compared to wild-type mice.Moreover,the NK cell activation was higher in the lungs of Ifitm3-/-mice during acute influenza infection.Conclusions Based on our results,we speculate that the NK cells are more readily activated in the absence of IFITM3,increasing mortality in Ifitm3-/-mice. | SUN Qiang LEI Na LU Jian GAO Rong Bao LI Zi LIU Li Qi SUN Ying GUO Jun Feng WANG Da Yan SHU Yue Long | 2020 | Biomedical and Environmental Sciences2020，33，5： | 4 |
| 4 | Effect of surfactants on the structure and photoelectric properties of ITO films by sol-gel method显示文摘The Indium tin oxide(ITO) thin film possesses excellent photoelectric properties that enable it to act as an ideal transparent conductor.To obtain high-quality ITO films through sol-gel method, the ionic surfactant monoethanolamine and the non-ionic surfactant polyethylene glycol(PEG) were added to the ITO precursor slurry.The influences of surfactants on the structural and photoelectric properties of ITO film samples were investigated.XRD patterns indicated that surfactant monoethanolamine contributed to film predominant grain orientation along the(400) plane.The high transmittance(over 95%) was attributed to the preferred orientation and the grain size expansion of ITO films.SEM showed that the surface particle size and the morphology of ITO films were strongly dependent on the kind of surfactants used.Moving to the shortwave region, the absorption edge of the films exhibited the Burstein-Moss shift. | LIU Jiaxiang, WU Da, ZHANG Nan, and WANG Yue College of Materials Science and Engineering, Beijing University of Chemical Technology, Beijing 100029, China | 2010 | Rare Metals2010，29，2： | 4 |
| 5 | A New Flavan-3-ol Glucoside from Daphniphyllum oldhami显示文摘A new flavan-3-ol glucoside, (-)-afzelechin-7-O-β-D-glucopyranoside (1), has been isolated from the stem of Daphniphyllum oldhami. The structure of the new compound was elucidated on the basis of detailed spectroscopic analysis and comparison with related compounds. | Zhi Yu SHAO, Da Yuan ZHU, Yue Wei GUOState Key Laboratory of Drug Research, Institute of Materia Medica, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031 | 2003 | Chinese Chemical Letters2003，14，9： | 3 |
| 6 | Analysis of microclimate characteristics in solar greenhouses under natural ventilation显示文摘The solar greenhouse is a typical greenhouse type in northern China.It provides a favorable environment for the growth of various plants and extends cultivation periods for almost a whole year to achieve a high yield.However,indoor environmental control is primarily based on growers'experience,and the objective test data required for the complex climate control and management of greenhouses are lacking.The present study used three greenhouses in northwest China as research objects:one greenhouse with mature plants(GH-M),one greenhouse with young plants(GH-Y)and one greenhouse without plants(GH-E).Field tests were performed to investigate microclimate characteristics,such as indoor air temperature,relative humidity,and solar radiation under natural ventilation.The results showed that the maximum temperature difference reached approximately 8.2℃in the vertical direction,and semiempirical equations for the normalized temperature distribution were obtained.The soil temperature remained constant at a certain depth(0.4 m).The distribution of the relative humidity and solar radiation was analyzed.The current study is helpful for growers to develop better greenhouse climate control strategies for management practices. | Yicun Hou Angui Li Yue Li Dacheng Jin Yu Tian Da Zhang Dingmeng Wu Linhua Zhang Wenjun Lei | 2021 | Building Simulation2021，14，6： | 3 |
| 7 | Insulin-like growth factor 1-induced enolase 2 deacetylation by HDAC3 promotes metastasis of pancreatic cancer显示文摘Enolase 2(ENO2)is a key glycolytic enzyme in the metabolic process of glycolysis,but its potential function in pancreatic ductal adenocarcinoma(PDAC)is unclear.In this study,we observed a significant overexpression of ENO2 in PDAC tissues,and its expression was correlated with metastasis and poor prognosis in PDAC patients.K394 was identified as a major acetylation site in ENO2 that regulates its enzymatic activity,cell metabolism and PDAC progression.Knockdown of ENO2 suppressed tumor growth and liver metastasis in PDAC.Re-expression of wild-type(WT)ENO2,but not the K394 acetylation mimetic mutant,could reverse the decreased tumor malignancy.We further characterized histone deacetylase 3(HDAC3)and P300/CBP-associated factor(PCAF)as the potential deacetylase and acetyltransferase for ENO2,respectively.HDAC3-mediated deacetylation was shown to lead to ENO2 activation and enhancement of glycolysis.Importantly,insulin-like growth factor-1(IGF-1)was found to decrease K394 acetylation and stimulate ENO2 activity in a dose-and time-dependent manner.The PI3K/AKT/mTOR pathway facilitated the phosphorylation of HDAC3 on S424,which promoted K394 deacetylation and activation of ENO2.Linsitinib,an oral small-molecule inhibitor of IGF-1R,could inhibit IGF-1-induced ENO2 deacetylation by HDAC3 and the PI3K/AKT/mTOR pathway.Furthermore,linsitinib showed a different effect on the growth and metastasis of PDAC depending on the overexpression of WT versus K394-mutant ENO2.Our results reveal a novel mechanism by which acetylation negatively regulates ENO2 activity in the metastasis of PDAC by modulating glycolysis.Blockade of IGF-1-induced ENO2 deacetylation represents a promising strategy to prevent the development of PDAC. | Yan Zheng Chao Wu Jimeng Yang Yue Zhao Huliang Jia Min Xue Da Xu Feng Yang Deliang Fu Chaoqun Wang Beiyuan Hu Ze Zhang Tianen Li Shican Yan Xuan Wang Peter J.Nelson Christiane Bruns Lunxiu Qin Qiongzhu Dong | 2020 | Signal Transduction and Targeted Therapy2020，5，1： | 3 |
| 8 | Identification of EGF as an Important Regulator for Promoting CYP3A4 Expression in Human Embryonic Stem Cell-Derived Hepatocytes Using TALEN-Based Gene Targeting显示文摘Functional human hepatocytes are one of the most significant tools for studying drug absorption,distribution,metabolism and excretion/toxicity (ADME/Tox),especially for applications in preclinical drug development (Sahi et al.,2010;Godoy et al.,2013).They provide the closest in vitro model to the human liver and the only model that mimics the drug metabolic profiles found in vivo.However,these cells lose their metabolic function rapidly and dramatically during the in vitro culture process,which largely hinders their wider application in drug development (Sahi et al.,2010;Godoy et al.,2013).To overcome this obstacle,it is important to regulate the activities of key genes which are responsible for the detoxification metabolic function of human hepatocytes. | Ming YinaCollege of Veterinary Medicine Jilin University Huan Yang Xiaohua Su Ziyi Li Zhanpeng Yue Xueming Zhang Da Sun Yan Shi Dexue Li | 2014 | Journal of Genetics and Genomics2014，41，6： | 2 |
| 9 | A modular microfluidic system based on a multilayered configuration to generate large-scale perfusable microvascular networks显示文摘The vascular network of the circulatory system plays a vital role in maintaining homeostasis in the human body.In this paper,a novel modular microfluidic system with a vertical two-layered configuration is developed to generate largescale perfused microvascular networks in vitro.The two-layer polydimethylsiloxane(PDMS)configuration allows the tissue chambers and medium channels not only to be designed and fabricated independently but also to be aligned and bonded accordingly.This method can produce a modular microfluidic system that has high flexibility and scalability to design an integrated platform with multiple perfused vascularized tissues with high densities.The medium channel was designed with a rhombic shape and fabricated to be semiclosed to form a capillary burst valve in the vertical direction,serving as the interface between the medium channels and tissue chambers.Angiogenesis and anastomosis at the vertical interface were successfully achieved by using different combinations of tissue chambers and medium channels.Various large-scale microvascular networks were generated and quantified in terms of vessel length and density.Minimal leakage of the perfused 70-kDa FITC-dextran confirmed the lumenization of the microvascular networks and the formation of tight vertical interconnections between the microvascular networks and medium channels in different structural layers.This platform enables the culturing of interconnected,large-scale perfused vascularized tissue networks with high density and scalability for a wide range of multiorgan-on-a-chip applications,including basic biological studies and drug screening. | Tao Yue Da Zhao Duc T.T.Phan Xiaolin Wang Joshua Jonghyun Park Zayn Biviji Christopher C.W.Hughes Abraham P.Lee | 2021 | Microsystems & Nanoengineering2021，7，1： | 2 |
| 10 | RosBREED:bridging the chasm between discovery and application to enable DNA-informed breeding in rosaceous crops显示文摘The Rosaceae crop family(including almond,apple,apricot,blackberry,peach,pear,plum,raspberry,rose,strawberry,sweet cherry,and sour cherry)provides vital contributions to human well-being and is economically significant across the U.S.In 2003,industry stakeholder initiatives prioritized the utilization of genomics,genetics,and breeding to develop new cultivars exhibiting both disease resistance and superior horticultural quality.However,rosaceous crop breeders lacked certain knowledge and tools to fully implement DNA-informed breeding—a“chasm”existed between existing genomics and genetic information and the application of this knowledge in breeding.The RosBREED project(“Ros”signifying a Rosaceae genomics,genetics,and breeding community initiative,and“BREED”,indicating the core focus on breeding programs),addressed this challenge through a comprehensive and coordinated 10-year effort funded by the USDA-NIFA Specialty Crop Research Initiative.RosBREED was designed to enable the routine application of modern genomics and genetics technologies in U.S.rosaceous crop breeding programs,thereby enhancing their efficiency and effectiveness in delivering cultivars with producer-required disease resistances and market-essential horticultural quality.This review presents a synopsis of the approach,deliverables,and impacts of RosBREED,highlighting synergistic global collaborations and future needs.Enabling technologies and tools developed are described,including genome-wide scanning platforms and DNA diagnostic tests.Examples of DNA-informed breeding use by project participants are presented for all breeding stages,including pre-breeding for disease resistance,parental and seedling selection,and elite selection advancement.The chasm is now bridged,accelerating rosaceous crop genetic improvement. | Amy F.Iezzoni Jim McFerson James Luby Ksenija Gasic Vance Whitaker Nahla Bassil Chengyan Yue Karina Gallardo Vicki McCracken Michael Coe Craig Hardner Jason D.Zurn Stan Hokanson Eric van de Weg Sook Jung Dorrie Main Cassia da Silva Linge Stijn Vanderzande Thomas M.Davis Lise L.Mahoney Chad Finn Cameron Peace | 2020 | Horticulture Research2020，7，1： | 2 |
| 11 | Environmental and tectonic significance of Late Permian reefs in the Linxi and adjacent areas in Inner Mongolia of China显示文摘Many plateau-edge reefs and reef mounds of the Late Permian have been discovered in Linxi County and adjacent areas of Inner Mongolia, China. These reefs verify that the Hinggan-Inner Mongolia Area was an open and unobstructed sea-trough in Late Permian. Facies and sequences of reef strata in Member 4 and Member 5 of the Linxi Formation were studied for information to reconstruct the closing process of the Linxi-Jiutai chasm in the sea-trough, i.e., to constrain subsidence, uplift, and final closing. Ages of the reef-bearing strata have been determined to be late and end of Late Permian, based on ranges or abundances of diverse fossils. Six reef-building organic assemblages were distinguished, representing different ecologic conditions, according to morphological functions and paleoecological adaptations of organisms including primarily sponges, bryozoans, and calcareous algae. Two types of shelf slopes, the gentle slope and the steep slope, have been interpreted for Member 4 and Member 5 of the Linxi Formation, based on the assemblages. The locations of outcrops, and types of these reefs delineate the north margin of Sino-Korea Platform, and define uplift and final closing of the Linxi-Jiutai chasm at the end of Late Permian. | TIAN Shu Gang ZHANG Yong Sheng GONG Yue Xuan LI Zi Shun GAO Lian Da ZHAI Da Xing ZHU Chang Wei | 2016 | Science China Earth Sciences2016，59，7： | 2 |
| 12 | Integrated Survivability Strategies of IP/GMPLS/Optical Multi-layer Network显示文摘In last decade,due to that the popularity of the internet, data-central traffic kept growing,some emerging networking requirements have been posed on the todays telecommunication networks,especially in the area of network survivability.Obviously,as a key networking problem,network reliability will be more and more important.The integration of different technologies such as ATM,SDH,and WDM in multilayer transport networks raises many questions regarding the coordination of the individual network layers.This problem is referred as multilayer network survivability.The integrated multilayer network survivability is investingated as well as the representation of an interworking strategy between different single layer survivability schemes in IP via generalized multi-protocol label switching over optical network. | ZHAO Ji jun 1,2 , JI Yue feng 1, XU Da xiong 1 (1. Opt.Commun.Center,Beijing University of Posts and telecommunications,Beijing 100876,CHN 2. Hebei Institute of Architectural Science and Technology, Handan 056038, CHN) | 2003 | Semiconductor Photonics and Technology2003，9，3： | 2 |
| 13 | Trace Nb-doped Na_(0.7)Ni_(0.3)Co_(0.1)Mn_(0.6)O_(2) with suppressed voltage decay and enhanced low temperature performance显示文摘The P2-type manganese-based Na_(0.7)MnO_(2) cathode materials attract great interest due to their high theoretical capacity.However,these materials suffer from rapid capacity fading,poor rate performance and severe voltage decay resulting from phase transition and sluggish reaction kinetics.In this work we report a novel Nb-doped Na_(0.7)[Ni_(0.3)Co_(0.1)Mn_(0.6)]_(1-x)Nb_(x)O_(2) with significantly suppre ssed voltage decay and enhanced cycling stability.The strong Nb-O bond can efficiently stabilize the TMO fra mework,and the as prepared material demonstrates much lower discharge midpoint voltage decay(0.132 V) than that of pristine one(0.319 V) after 200 cycles.Consequently,a remarkably improved cycling perfo rmance with a capacity retention of 87.9% after 200 cycle at 0.5 C is achieved,showing a 2.4 fold improvement as compared to the control sample Na_(0.7)Ni_(0.3)Co_(0.1)Mn_(0.6)O_(2)(~37% rotation).Even at 2 C,a capacity retention of 68.4% is retained after 500 cycles.Remarkably,the as prepared material can be applied at low temperature of-20℃,showing a capacity retention of 81% as compared to that at room temperature. | Ruyun Yue Fang Xia Ruijuan Qi Da Tie Shanshan Shi Zhiping Li Yufeng Zhao Jiujun Zhang | 2021 | Chinese Chemical Letters2021，32，2： | 2 |
| 14 | Deciphering the chromatin spatial organization landscapes during BMMSC differentiation显示文摘The differentiation imbalance in bone marrow mesenchymal stem cells(BMMSCs)is critical for the development of bone density diseases as the population ages.BMMSCs are precursor cells for osteoblasts and adipocytes;however,the chromatin organization landscapes during BMMSC differentiation remain elusive.In this study,we systematically delineate the four-dimensional genome and dynamic epigenetic atlas of BMMSCs by RNA sequencing,assay for transposase-accessible chromatin sequencing,and highthroughput chromosome conformation capture.The structure analyses reveal 17.5%common and28.5%-30%specific loops among BMMSCs,osteoblasts,and adipocytes.The subsequent correlation of genome-wide association studies and expression quantitative trait locus(e QTL)data with multi-omics analysis reveal 274 genes and 3634 single nucleotide polymorphisms(SNPs)associated with bone degeneration and osteoporosis(OP).We hypothesize that SNP mutations affect transcription factor(TF)binding sites,thereby affecting changes in gene expression.Furthermore,26 motifs,260 TFs,and 291SNPs are identified to affect the e QTL.Among these genes,DAAM2,TIMP2,and TMEM241 are found to be essential for diseases such as bone degeneration and OP and may serve as potential drug targets. | Zhaowei Teng Yun Zhu Da Lin Qinggang Hao Qiaoning Yue Xiaochao Yu Shuo Sun Lihong Jiang Sheng Lu | 2023 | Journal of Genetics and Genomics2023，50，4： | 1 |
| 15 | Tracing type 1 diabetic T ibet miniature pig’s bone marrow mesenchymal stem cells in vitro by magnetic resonance imaging (1型糖尿病西藏小型猪骨髓间充质干细胞体外磁共振成像示踪)显示文摘 | Kuan‐Xiao Tang Jin‐Hua Yan Yun‐Feng Shen Bao‐Ying Li Ying‐Ming Chen Da‐Yue Liu De‐Dong Ma Jie Li Hua Liang Jian‐Ping Weng | 2014 | Journal of Diabetes2014，，： | 1 |
| 16 | On the Monotonicity of the Speed of Random Walks on a Percolation Cluster of Trees显示文摘作者在树的 Bernoulli 契约过滤的无限的簇上考虑简单随机的散步,并且由学习树的三个班调查在简单随机的散步和保留的概率 p 的速度之间的关系。一个足够的条件为 Galton-Watson 树被建立。 | Da Yue CHEN Fu Xi ZHANG | 2007 | Acta Mathematica Sinica,English Series2007，23，11： | 1 |
| 17 | Influence of aortic branch arteries on the hemodynamics of patient-specific type B aortic dissection following TEVAR显示文摘Objective:Because of the difficulty in obtaining patient specific flow data,branch arteries are often neglected in the flow simulations of a patient-specific type B aortic dissection(AD)following thoracic endovascular repair(TEVAR).This study aims to investigate the influence of the aortic branch arteries on the hemodynamics and physical flow phenomena commonly associated with aortic modeling of an AD after TEVAR.Methods:The three-dimensional geometry of a type B AD following TEVAR was generated from a high-quality CT dataset.Simulations were performed with all branch arteries,without the four visceral arteries of the abdominal aorta(FVAoAA),and without the upper three branches of the aortic arch(UTBoAA).Results:The removal of the UTBoAA has marginal effect on the flow patterns and hemodynamic parameters in the false lumen(FL);however,regardless of whether there are aorta branches supplied to the FL or not,the omission of FVAoAA will result in a wrong prediction of the flow direction at tears and flow volume to the FL.Conclusions:The omission of aorta branch arteries has great impact on the hemodynamic prediction including flow field and wall shear stress related parameters in a type B AD after TEVAR,which may induce misleading estimates of the potential thrombus in the FL.It is strongly recommended to keep aorta branches in the computational model to predict the post-operative hemodynamic performance in a type B AD following TEVAR. | Yi Jiang Yue Qiu Da Li Ding Yuan Tinghui Zheng Liqing Peng | 2019 | Medicine in Novel Technology and Devices2019，，4： | 1 |
| 18 | Functional somatotopy revealed across multiple cortical regions using a model of complex motor task显示文摘 | Cunningham DA Machado A Yue GH | 2013 | Brain Res2013，19，9： | 1 |
| 19 | Two New Triterpene Esters from Daphniphyllum oldhami显示文摘Two new triterpene esters, 2-O-caffeoylalphitolic acid (1) and methyl 2-O-caffeoyl- alphitolate (2), together with two known triterpenes were isolated from the shrub Daphniphyllum oldhami. The structures of new compounds were elucidated on the basis of detailed spectroscopic analysis and compared with related compound. | Zhi Yu SHAO, Da Yuan ZHU, Yue Wei GUO* State Key Laboratory of Drug Research, Institute of Materia Medica, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031 | 2002 | Chinese Chemical Letters2002，13，12： | 1 |
| 20 | Axonal protection in experimental autoimmune neuritis by the sodium channel blocking agent flecainide显示文摘 | Bechtold DA Yue X Evans RM | 2005 | Brain2005，128，： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员