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| 1 | UFMylation of RPL26 links translocation-associated quality control to endoplasmic reticulum protein homeostasis显示文摘Protein biogenesis at the endoplasmic reticulum(ER)in eukaryotic cells is monitored by a protein quality control system named ER-associated protein degradation(ERAD).While there has been substantial progress in understanding how ERAD eliminates defective polypeptides generated from erroneous folding,how cells remove nascent chains stalled in the translocon during co-translational protein insertion into the ER is unclear.Here we show that ribosome stalling during protein translocation induces the attachment of UFM1,a ubiquitin-like modifier,to two conserved lysine residues near the COOH-terminus of the 60S ribosomal subunit RPL26(uL24)at the ER.Strikingly,RPL26 UFMylation enables the degradation of stalled nascent chains,but unlike ERAD or previously established cytosolic ribosome-associated quality control(RQC),which uses proteasome to degrade their client proteins,ribosome UFMylation promotes the targeting of a translocation-arrested ER protein to lysosomes for degradation.RPL26 UFMylation is upregulated during erythroid differentiation to cope with increased secretory flow,and compromising UFMylation impairs protein secretion,and ultimately hemoglobin production.We propose that in metazoan,co-translational protein translocation into the ER is safeguarded by a UFMylation-dependent protein quality control mechanism,which when impaired causes anemia in mice and abnormal neuronal development in humans. | Lihui Wang Yue Xu Heather Rogers Layla Saidi Constance Tom Noguchi Honglin Li Jonathan Wilson Yewdell Nicholas Raymond Guydosh Yihong Ye | 2020 | Cell Research2020，30，1： | 5 |
| 2 | Maize ZmVPP5 is a truncated Vacuole H^+-PPase that confers hypersensitivity to salt stress显示文摘In plants, Vacuole H^+-PPases(VPPs) are important Aproton pumps and encoded by multiple genes. In addition to full-length VPPs, several truncated forms are expressed, but their biological functions are unknown. In this study, we functionally characterized maize vacuole H^+-PPase 5(ZmVPP5), a truncated VPP in the maize genome. Although ZmVPP5 shares high sequence similarity with ZmVPP1 ZmVPP5 lacks the complete structure of the conserved proton transport and the inorganic pyrophosphatase-related domain. Phylogenetic analysis suggests that ZmVPP5 might be derived from an incomplete gene duplication event. ZmVPP5 is expressed in multiple tissues, and ZmVPP5 was detected in the plasma membrane, vacuole membrane and nuclei of maize cells. The overexpression of ZmVPP5 in yeast cells caused a hypersensitivity to salt stress. Transgenic maize lines with overexpressed ZmVPP5 also exhibited the salt hypersensitivity phenotype. A yeast two-hybrid analysis identified the ZmBag6 like protein as a putative ZmVPP5-interacting protein. The results of bimolecular luminescence complementation(Bi LC)assay suggest an interaction between ZmBag6-like protein and ZmVPP5 in vivo. Overall, this study suggests that ZmVPP5 might act as a VPP antagonist and participate in the cellular response to salt stress. Our study of ZmVPP5 has expanded the understanding of the origin and functions of truncated forms of plant VPPs. | Xiaoliang Sun Weiwei Qi Yihong Yue Huiling Ling Gang Wang Rentao Song | 2016 | Journal of Integrative Plant Biology2016，58，6： | 2 |
| 3 | The First High-quality Reference Genome of Sika Deer Provides Insights into High-tannin Adaptation显示文摘Sika deer are known to prefer oak leaves,which are rich in tannins and toxic to most mammals;however,the genetic mechanisms underlying their unique ability to adapt to living in the jungle are still unclear.In identifying the mechanism responsible for the tolerance of a highly toxic diet,we have made a major advancement by explaining the genome of sika deer.We generated the first high-quality,chromosome-level genome assembly of sika deer and measured the correlation between tannin intake and RNA expression in 15 tissues through 180 experiments.Comparative genome analyses showed that the UGT and CYP gene families are functionally involved in the adaptation of sika deer to high-tannin food,especially the expansion of the UGT family 2 subfamily B of UGT genes.The first chromosome-level assembly and genetic characterization of the tolerance to a highly toxic diet suggest that the sika deer genome may serve as an essential resource for understanding evolutionary events and tannin adaptation.Our study provides a paradigm of comparative expressive genomics that can be applied to the study of unique biological features in non-model animals. | Xiumei Xing Cheng Ai Tianjiao Wang Yang Li Huitao Liu Pengfei Hu Guiwu Wang Huamiao Liu Hongliang Wang Ranran Zhang Junjun Zheng Xiaobo Wang Lei Wang Yuxiao Chang Qian Qian Jinghua Yu Lixin Tang Shigang Wu Xiujuan Shao Alun Li Peng Cui Wei Zhan Sheng Zhao Zhichao Wu Xiqun Shao Yimeng Dong Min Rong Yihong Tan Xuezhe Cui Shuzhuo Chang Xingchao Song Tongao Yang Limin Sun Yan Ju Pei Zhao Huanhuan Fan Ying Liu Xinhui Wang Wanyun Yang Min Yang Tao Wei Shanshan Song Jiaping Xu Zhigang Yue Qiqi Liang Chunyi Li Jue Ruan Fuhe Yang | 2023 | Genomics, Proteomics & Bioinformatics2023，21，1： | 1 |
| 4 | Light-switchable catalytic activity of Cu for oxygen reduction reaction显示文摘The surface reactivity of metals is fundamentally dependent on the local electronic structure generally tailored by atomic compositions and configurations during the synthesis.Herein,we demonstrate that Cu,which is inert for oxygen reduction reaction(ORR)due to the fully occupied d-orbital,could be activated by applying a visible-light irradiation at ambient temperature.The ORR current is increased to 3.3 times higher in the potential range between-0.1 and 0.4 V under the light of 400 mW·cm^-2,and the activity enhancement is proportional to the light intensity.Together with the help of the first-principle calculation,the remarkably enhanced electrocatalytic activity is expected to stem mainly from the decreased metal-adsorbate binding by photoexcita-tion.This finding provides an additional degree of freedom for controlling and manipulating the surface reactivity of metal catalysts besides materials strategy. | Yue ZHANG Yihong YU Xiankai FU Zhisen LIU Yinglei LIU Song LI | 2020 | Frontiers of Materials Science2020，14，4： | 0 |
| 5 | Antibacterial Activity of Novel 18β-Glycyrrhetinic Hydrazide or Amide Derivatives显示文摘Through a facile structural modification on the natural bioactive ingredient 18β-glycyrrhetinic acid(GA),a series of novel GA hydrazide or amide derivatives was obtained,and their final molecular frameworks were characterized by NMR and HRMS analysis.Antibacterial bioassays revealed that some of the GA hydrazide or amide derivatives were able to suppress the growth of three tested plant pathogens.Particularly,compound 3c exhibited excellent in vitro activity against Xanthomonas oryzae pv.Oryzae(Xoo),Pseudomonas syringae pv.actinidiae(Psa),and Xanthomonas axonopodis pv.citri(Xac),providing the EC_(50) values of 5.89,16.1,and 3.64µg/mL,respectively.The data were better than those of the positive controls thiodiazole copper(92.7,77.8,and 89.9µg/mL,respectively)and bismerthiazol(31.1,125.6,and 77.4µg/mL,respectively).In addition,in vivo experiments suggested that,compared with thiodiazole copper(41.93%and 39.73%,respectively),compound 3c exerted prominently curative and protective activities against rice bacterial leaf blight at 200µg/mL with the control effects of 52.36%and 51.40%,respectively.Given these obtained results,GA hydrazide or amide derivatives could serve as the feasible leads for exploring highly bioactive substrates. | ZHANG Ling FU Yihong DING Yue MENG Jiao WANG Zhenchao WANG Peiyi | 2021 | Chemical Research in Chinese Universities2021，37，3： | 0 |
| 6 | Influence of cyclic dynamic disturbance on damage evolution and zoning effect of coal-rock under local static load constraint显示文摘In order to obtain the characteristics of the effects of cyclic impact loading on the damage of coal-rock in the presence of a local static load constraint,the evolution of the damage factor and the fracture rate during the process and incremental cyclic impact on raw coal and briquettes has been studied.Experimental results show that the presence of local static load restraint improves the impact resistance of the coal-rock,and the damage factor of the coal-rock shows obvious zoning characteristics.When the coal-rock is in an elastic state,the partition with a larger static load restraint area has stronger impact resistance,when the coal-rock is in a plastic state,the partition with a larger static load restraint area has a weaker impact resistance.Increasing impulsive cyclic impacts have a higher damage efficiency to coal-rock than constant impulsive cyclic impacts.The difference in rock breaking efficiency between the two cyclic impact methods is mainly reflected in the partition with the largest constrained area.The crack propagation on the coal-rock surface is more consistent with the partition characteristics of the damage factor.When the static load constrained zone is in an elastic state,the static load has an inhibitory effect on the crack growth.When the static load confinement zone is in a plastic state,the cracks mainly propagate in the static load confinement zone,and the constrained zone mainly consists of tensile cracks that grow in the vertical direction,while the cracks in the non-constrained zone mainly grow in an oblique direction.Finally,fracture mechanics was applied to analyze the failure type of the sample. | Yihong Liu Hongbao Zhao Aiwen Wang Lianpeng Dai Yue Li Hongwei Zhang | 2023 | Geohazard Mechanics2023，1，4： | 0 |
| 7 | Quantification of TOC and TN in reservoir sediments using Fourier transform infrared spectroscopy显示文摘This study aims to quantitatively assess the total organic carbon(TOC)and total nitrogen(TN)content of reservoir sediments in southwest China using Fourier transform infrared spectroscopy(FTIRS).FTIRS measurements were performed on 187 sediment samples from four reservoirs to develop calibration models that relate FTIR spectral information with conventional property concentrations using partial least squares regression(PLSR).Robust calibration models were established for TOC and TN content.The external validation of these models yielded a significant correlation between FTIR-inferred and conventionally measured concentrations of R^(2)=0.88 for TOC,R^(2)=0.90 for TN.This method can be performed with a small sample size and is non-destructive throughout the simple measurement process.The TOC and TN content in the sediment can be determined with high effectiveness without being overly expensive,making it an advantageous method when measuring a large number of samples. | Lin Mei Yihong Yue Yong Qin Xueping Chen Fushun Wang | 2022 | Soil Ecology Letters2022，4，4： | 0 |
| 8 | Coupling effects of hydrological characteristics and nutrient load in sediments on the trophic state of reservoirs显示文摘Currently,lakes and artificial reservoirs are increasingly threatened by eutrophication,which is the result of the combined action of many natural and anthropogenic factors.In the past,the effect of nutrient load on the trophic state of water bodies has attracted much attention,while few studies have addressed the effect of hydrological characteristics.Therefore,to reveal the coupling effects of hydrological characteristics and nutrient load in sediments on the trophic state of water bodies,this study collected relevant data from 36 lakes and reservoirs across China.Pearson correlation analysis showed that trophic level index was positively and significantly correlated with nutrient load in sediments and hydraulic retention time,while it was negatively correlated with mean depth and hydraulic load.The principal component analysis showed that the nutrient load was the first major component that influenced the trophic state of water bodies,followed by the mean depth and hydraulic retention time.Eutrophication was prone to occur in water bodies with mean depth less than 7 m and hydraulic retention time greater than 14 d,and the trophic level index regression equation with hydrological characteristics and nutrient load in sediments was derived by multiple regression analysis.This study revealed that the trophic state of water bodies influenced by both nutrient load and hydrological characteristics.It provides a new idea to reduce the occurrence of eutrophication in reservoirs by using the artificial hydrological regulation capacity of reservoirs. | Fan Xinyi Yue Yihong Mei Lin Li Xiaoying Hu Zhehui Luo Jiajie Liu Liu Wang Fushun | 2021 | Acta Geochimica2021，40，4： | 0 |
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