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| 1 | The Property of Solution of a Nonstationary Forest Evolution System | WANG Dingjiang Department of Mathematics, Pingdingshan Teacher’s College, He’nan 467000ZHANG Yufeng Department of Mathematics, Yanbian Teacher’s College, Jilin 133000 | 1993 | Systems Science and Systems Engineering1993，3，3： | 23 |
| 2 | Synergistic effect of fluorination on both donor and acceptor materials for high performance non-fullerene polymer solar cells with 13.5% efficiency显示文摘A high performance polymer solar cells(PSCs) based on polymer donor PM6 containing fluorinated thienyl benzodithiophene unit and n-type organic semiconductor acceptor IT-4 F containing fluorinated end-groups were developed. In addition to complementary absorption spectra(300–830 nm) with IT-4 F, the PM6 also has a deep HOMO(the highest occupied molecular) level(-5.50 e V), which will lower the open-circuit voltage(V_(oc)) sacrifice and reduce the E_(loss) of the IT-4 F-based PSCs. Moreover, the strong crystallinity of PM6 is beneficial to form favorable blend morphology and hence to suppress recombination. As a result, in comparison with the PSCs based on a non-fluorinated D/A pair of PBDB-T:ITIC with a medium PCE of 11.2%, the PM6:IT-4 Fbased PSCs yielded an impressive PCE of 13.5% due to the synergistic effect of fluorination on both donor and acceptor, which is among the highest values recorded in the literatures for PSCs to date. Furthermore, a PCE of 12.2% was remained with the active layer thickness of up to 285 nm and a high PCE of 11.4% was also obtained with a large device area of 1 cm^2. In addition, the devices also showed good storage, thermal and illumination stabilities with respect to the efficiency. These results indicate that fluorination is an effective strategy to improve the photovoltaic performance of materials, as well as the both fluorinated donor and acceptor pair-PM6:IT-4 F is an ideal candidate for the large scale roll-to-roll production of efficient PSCs in the future. | Qunping Fan Wenyan Su Yan Wang Bing Guo Yufeng Jiang Xia Guo Feng Liu Thomas P.Russell Maojie Zhang Yongfang Li | 2018 | Science China Chemistry2018，61，5： | 18 |
| 3 | Consensus on the application of negative pressure wound therapy of diabetic foot wounds显示文摘Because China is becoming an aging society,the incidence of diabetes and diabetic foot have been increasing.Diabetic foot has become one of the main health-related killers due to its high disability and mortality rates.Negative pressure wound therapy(NPWT)is one of the most effective techniques for the treatment of diabetic foot wounds and great progress,both in terms of research and its clinical application,has been made in the last 20 years of its development.However,due to the complex pathogenesis and management of diabetic foot,irregular application of NPWT often leads to complications,such as infection,bleeding and necrosis,that seriously affect its treatment outcomes.In 2020,under the leadership of Burns,Trauma and Tissue Repair Committee of the Cross-Straits Medicine Exchange Association,the writing group for‘Consensus on the application of negative pressure wound therapy of diabetic foot wounds’was established with the participation of scholars from the specialized areas of burns,endocrinology,vascular surgery,orthopedics and wound repair.Drawing on evidence-based practice suggested by the latest clinical research,this consensus proposes the best clinical practice guidelines for the application and prognostic evaluation of NPWT for diabetic foot.The consensus aims to support the formation of standardized treatment schemes that clinicians can refer to when treating cases of diabetic foot. | Shizhao Ji Xiaobin Liu Jie Huang Junmin Bao Zhaohong Chen Chunmao Han Daifeng Hao Jingsong Hong Dahai Hu Yufeng Jiang Shang Ju Hongye Li Zongyu Li Guangping Liang Yan Liu Gaoxing Luo Guozhong Lv Xingwu Ran Zhongmin Shi Juyu Tang Aiping Wang Guangyi Wang Jiangning Wang Xin Wang Bing Wen Jun Wu Hailin Xu Maojin Xu Xiaofei Ye Liangxi Yuan Yi Zhang Shichu Xiao Zhaofan Xia | 2021 | Burns & Trauma2021，9，1： | 17 |
| 4 | Pm21 from Haynaldia villosa Encodes a CC-NBS- LRR Protein Conferring Powdery Mildew Resistance in Wheat显示文摘 | Liping Xing Ping Hu Jiaqian Liu Kamil Witek Shuang Zhou Jiefei Xu Weihao Zhou Li Gao Zhenpu Huang Ruiqi Zhang Xiue Wang Peidu Chen Haiyan Wang Jonathan D.G. Jones Miroslava Karafiatova Jan Vrana Jan Baros Jaroslav Dolezel Yuanchun Tian Yufeng Wu Aizhong Cao | 2018 | Molecular Plant2018，11，6： | 16 |
| 5 | A simple and efficient method for CRISPR/Cas9-induced mutant screening显示文摘The clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated protein 9(Cas9) system provides a technological breakthrough in mutant generation. Several methods such as the polymerase chain reaction(PCR)/restriction enzyme(RE) assay, T7 endonuclease I(T7EI) assay, Surveyor nuclease assay, PAGE-based genotyping assay, and high-resolution melting(HRM) analysis-based assay have been developed for screening CRISPR/Cas9-induced mutants. However, these methods are timeand labour-intensive and may also be sequence-limited or require very expensive equipment. Here, we described a cost-effective and sensitive screening technique based on conventional PCR, annealing at critical temperature PCR(ACT-PCR), for identifying mutants. ACT-PCR requires only a single PCR step followed by agarose gel electrophoresis. We demonstrated that ACT-PCR accurately distinguished CRISPR/Cas9-induced mutants from wild type in both rice and zebrafish. Moreover, the method can be adapted for accurately determining mutation frequency in cultured cells. The simplicity of ACT-PCR makes it particularly suitable for rapid, large-scale screening of CRISPR/Cas9-induced mutants in both plants and animals. | Yufeng Hua Chun Wang Jian Huang Kejian Wang | 2017 | Journal of Genetics and Genomics2017，44，4： | 16 |
| 6 | Impact of anthropogenic activities on the sediment microbial communities of Baiyangdian shallow lake显示文摘Microorganisms are recognized as sensors of external disturbance in freshwater ecosystems.In this study,sediment samples from regions with strong human activity interference(four sites),moderate human activity interference(five sites)and minor human activity interference(five sites)in Baiyangdian Lake were collected;and their physiological and chemical properties and heavy metals contents were analyzed.The structures of bacterial and fungal communities were tested using 16S ribosomal Ribonucleic Acid(rRNA)and Internal Transcribed Spacer 2(ITS2)high-throughput sequencing technologies.The results indicated that Proteobacteria was the most abundant bacterial phylum.Ascomycota,Basidiomycota and Ciliophora were the dominant phyla of fungi.Obvious differences of microbial genera among the three regions were observed,especially fungal genera.With intensified disturbance by human activities,the Simpson and Shannon indices of bacteria significantly decreased.The fungal communities were clustered into three groups in accordance with the different human disturbance levels.The bacterial communities of sediment samples under moderate and minor human disturbance appeared similar,but different from those under strong human disturbance.Redundancy analysis indicated that zinc,total phosphorus,copper,and lead were the dominant factors that influenced the bacterial community.Nitrifiers and sulfate-reducing bacteria were influenced by pH,nitrate(NO3 ),and total nitrogen.The fungal community was significantly affected by total phosphorus.This study reveals that sediment microbial community structures among different regions of a shallow lake are mainly impacted by human activities. | Wenjun Wang Yujun Yi Yufeng Yang Yang Zhou Shanghong Zhang Xuan Wang Zhifeng Yang | 2020 | International Journal of Sediment Research2020，35，2： | 14 |
| 7 | Nat Microbiol:已结首次构建出小鼠肠道微生物组数据库显示文摘小鼠模型通常被广泛用于药理学和医学研究。我们都知道小鼠肠道中的微生物群落通常会对研究结果产生明显的影响,然而目前研究者们并没有获取到关于小鼠肠道中栖息细菌群落的详细信息。近日刊登在国际杂志Nature Microbiology上的一篇研究报告中。德国慕尼黑理工大学的研究者通过研究首次收集到了小鼠肠道中的多种细菌群落。同时研究者还对其进行了分裂、特性分析。最后得到了100种细菌,其中还包括15种迄今为止未知的细菌群落。 | Lu, Qiulun Yao, Yufeng Hu, Zhenkun Hu, Changqing Song, Qixue Ye, Jian Xu, Chengqi Wang, Annabel Z. Chen, Qiuyun Wang, Qing Kenneth | 2016 | 现代生物医学进展2016，16，32： | 13 |
| 8 | Antibacterial effects of platelet-rich fibrin produced by horizontal centrifugation显示文摘Platelet-rich fibrin(PRF)has been widely used owing to its ability to stimulate tissue regeneration.To date,few studies have described the antibacterial properties of PRF.Previously,PRF prepared by horizontal centrifugation(H-PRF)was shown to contain more immune cells than leukocyte-and platelet-rich fibrin(L-PRF).This study aimed to compare the antimicrobial effects of PRFs against Staphylococcus aureus and Escherichia coli in vitro and to determine whether the antibacterial effects correlated with the number of immune cells.Blood samples were obtained from eight healthy donors to prepare L-PRF and H-PRF.The sizes and weights of L-PRF and H-PRF were first evaluated,and their antibacterial effects against S.aureus and E.coli were then tested in vitro using the inhibition ring and plate-counting test methods.Flow-cytometric analysis of the cell components of L-PRF and H-PRF was also performed.No significant differences in size or weight were observed between the L-PRF and H-PRF groups.The H-PRF group contained more leukocytes than the L-PRF group.While both PRFs had notable antimicrobial activity against S.aureus and E.coli,H-PRF demonstrated a significantly better antibacterial effect than L-PRF.Furthermore,the antimicrobial ability of the PRF solid was less efficient than that of wet PRF.In conclusion,H-PRF exhibited better antibacterial activity than L-PRF,which might have been attributed to having more immune cells. | Mengge Feng Yulan Wang Peng Zhang Qin Zhao Shimin Yu Kailun Shen Richard J.Miron Yufeng Zhang | 2020 | International Journal of Oral Science2020，12，4： | 12 |
| 9 | Three pairs of alkaloid enantiomers from the root of Isatis indigotica显示文摘Three pairs of enantiomerically pure alkaloids with diverse structure features, named isatindigoticoic acid A and epiisatindigoticoic acid A [(—)-1 and(+)-1], phaitanthrin A and epiphaitanthrin A [(—)-2 and(+)-2], and isatindopyrromizol A and epiisatindopyrromizol A [(—)-3and(+)-3], respectively, were isolated from an aqueous extract of the roots of Isatis indigotica. Racemic and scalemic mixtures of these enantiomers were separated by HPLC on a chiral semi-preparative column.Their structures including absolute configurations were determined by extensive spectroscopic analysis in conjunction with the calculation of electronic circular dichroism(ECD) spectra. The enantiomer pairs possess parent structures of 2-oxo-1,2,3,4-tetrahydroquinoline-4-carboxylic acid, indolo[2,1-b]quinazolinone, and 3-thioxohexahydro-1H-pyrrolo[1,2-c]imidazol-1-one, respectively. Except for phaitanthrin A[(—)-2] which the configuration was previously undetermined, these compounds are new enantiomeric natural products. | Yufeng Liu Xiaoliang Wang Minghua Chen Sheng Lin Li Li Jiangong Shi | 2016 | Acta Pharmaceutica Sinica B2016，6，2： | 12 |
| 10 | Clinical characteristics and effectiveness of antiviral agents in hospitalized children with infectious mononucleosis in China:A multicenter retrospective study显示文摘Importance:The clinical characteristics of infectious mononucleosis(IM)in Chinese children have not been evaluated in multicenter studies,and the effectiveness of antiviral treatment are controversial.Objective:To investigate the clinical characteristics of Chinese children with IM and current status of antiviral therapy for affected patients.Methods:Hospitalized patients with IM were enrolled between 2018 and 2020 in five children’s hospitals in China.The clinical characteristics were compared among four age groups:<3 years,3-<6 years,6-<10 years,and≥10 years.The clinical characteristics of IM and effectiveness of antiviral therapy were compared among patients receiving acyclovir(ACV),ganciclovir(GCV),and no antiviral therapy(i.e.,non-antiviral group).Results:In total,499 patients were analyzed;most patients were 3-<6 years of age.The most common symptoms and signs included fever(100%),lymphadenopathy(98.6%),pharyngitis(86.4%),eyelid edema(76.8%),and snoring(72.9%).There were significant differences in rash,hepatomegaly,and liver dysfunction among the four age groups.Patients aged<3 years had a lower incidence of liver dysfunction and a higher incidence of rash.Among the 499 patients,50.1%were treated with GCV,26.3%were treated with ACV,and 23.6%received no antiviral therapy.Compared with the non-antiviral group,patients in the ACV and GCV groups had longer durations of fever(P<0.001).There were no significant differences in the incidences of complications among the three treatment groups.Interpretation:The incidence of IM in Chinese children peaked at 3-<6 years of age.Clinical features of IM varied according to age.Patients receiving antiviral therapy exhibited more serious clinical manifestations than did patients without antiviral therapy.The effectiveness of antiviral therapy for IM requires further analysis. | Huili Hu Huiling Deng Jing Bi Yi Xu Shuangjie Li Yue Xie Xinrong Sun Dongmeng Wang Xufang Li Wenxian Ouyang Bing Hu Yufeng Zhang He Tang Chunxiao Fang Hui Zhang Lingyun Guo Chen Wang Tianyi Wang Fengxia Yang Tao Jiang Zhengde Xie Gang Liu | 2021 | Pediatric Investigation2021，5，3： | 12 |
| 11 | Growth response and toxic effects of three antibiotics on Selenastrum capricornutum evaluated by photosynthetic rate and chlorophyll biosynthesis显示文摘The effects of three types of antibiotics (erythromycin,ciprofloxacin and sulfamethoxazole) on the photosynthesis of freshwater algae,Selenastrum capricornutum Printz,were investigated by determining the growth rate,chloroplast pigments content,seven main precursors (including δ-aminolevulinic acid,porphobilinogen,uroporphyrinogen III,coproporphyrinogen III,protoporphyrin IX,Mg-proporphyrin IX and protochlorophyllide),and photosynthetic rate during chlorophyll biosynthesis.The antibiotics significantly decreased the growth rate,chlorophyll content,and photosynthetic rate.Erythromycin induced a decreasing effect at a concentration of 0.06 mg/L,while ciprofloxacin and sulfamethoxazole achieved the same results at concentrations higher than 1.5 mg/L.Only erythromycin significantly inhibited chlorophyll biosynthesis,which indicated that it was considerably more toxic to S.capricornutum than ciprofloxacin and sulfamethoxazole,and may pose a high potential risk to aquatic ecosystems. | Binyang Liu Weiqiu Liu Xiangping Nie Chao Guan Yufeng Yang Zhaohui Wang Wei Liao | 2011 | Journal of Environmental Sciences2011，23，9： | 11 |
| 12 | Rapid generation of genetic diversity by multiplex CRISPR/Cas9 genome editing in rice显示文摘The clustered regularly interspaced short palindromic repeats(CRISPR)-associated endonuclease 9(CRISPR/Cas9) system has emerged as a promising technology for specific genome editing in many species. Here we constructed one vector targeting eight agronomic genes in rice using the CRISPR/Cas9 multiplex genome editing system. By subsequent genetic transformation and DNA sequencing, we found that the eight target genes have high mutation efficiencies in the T_0 generation. Both heterozygous and homozygous mutations of all editing genes were obtained in T_0 plants. In addition, homozygous sextuple, septuple, and octuple mutants were identified. As the abundant genotypes in T_0 transgenic plants, various phenotypes related to the editing genes were observed. The findings demonstrate the potential of the CRISPR/Cas9 system for rapid introduction of genetic diversity during crop breeding. | Lan Shen Yufeng Hua Yaping Fu Jian Li Qing Liu Xiaozhen Jiao Gaowei Xin Junjie Wang Xingchun Wang Changjie Yan Kejian Wang | 2017 | Science China(Life Sciences)2017，60，5： | 11 |
| 13 | Personalized Real-Time Movie Recommendation System:Practical Prototype and Evaluation显示文摘With the eruption of big data,practical recommendation schemes are now very important in various fields,including e-commerce,social networks,and a number of web-based services.Nowadays,there exist many personalized movie recommendation schemes utilizing publicly available movie datasets(e.g.,MovieLens and Netflix),and returning improved performance metrics(e.g.,Root-Mean-Square Error(RMSE)).However,two fundamental issues faced by movie recommendation systems are still neglected:first,scalability,and second,practical usage feedback and verification based on real implementation.In particular,Collaborative Filtering(CF)is one of the major prevailing techniques for implementing recommendation systems.However,traditional CF schemes suffer from a time complexity problem,which makes them bad candidates for real-world recommendation systems.In this paper,we address these two issues.Firstly,a simple but high-efficient recommendation algorithm is proposed,which exploits users1 profile attributes to partition them into several clusters.For each cluster,a virtual opinion leader is conceived to represent the whole cluster,such that the dimension of the original useritem matrix can be significantly reduced,then a Weighted Slope One-VU method is designed and applied to the virtual opinion leader-item matrix to obtain the recommendation results.Compared to traditional clusteringbased CF recommendation schemes,our method can significantly reduce the time complexity,while achieving comparable recommendation performance.Furthermore,we have constructed a real personalized web-based movie recommendation system,MovieWatch,opened it to the public,collected user feedback on recommendations,and evaluated the feasibility and accuracy of our system based on this real-world data. | Jiang Zhang Yufeng Wang Zhiyuan Yuan Qun Jin | 2020 | Tsinghua Science and Technology2020，25，2： | 10 |
| 14 | DZNep inhibits the proliferation of colon cancer HCT116 cells by inducing senescence and apoptosis显示文摘EZH2 is over-expressed in human colon cancer and is closely associated with tumor proliferation,metastasis and poor prognosis.Targeting and inhibiting EZH2 may be an effective therapeutic strategy for colon cancer.3-Deazaneplanocin A(DZNep),as an EZH2 inhibitor,can suppress cancer cell growth.However,the anti-cancer role of DZNep in colon cancer cells has been rarely studied.In this study,we demonstrate that DZNep can inhibit the growth and survival of colon cancer HCT116 cells by inducing cellular senescence and apoptosis.The study provides a novel view of anti-cancer mechanisms of DZNep in human colon cancer cells. | Mingquan Sha Genxiang Mao Guofu Wang Yufeng Chen Xiaojian Wu Zhen Wang | 2015 | Acta Pharmaceutica Sinica B2015，5，3： | 10 |
| 15 | Regulatory effects of antitumor agent matrine on FOXO and PI3K-AKT pathway in castration-resistant prostate cancer cells显示文摘We previously demonstrated that matrine could inhibit the proliferating, migrating, as well as invading processes of both PC-3 and DU145 cells. However, the underlying molecular mechanisms have not yet been clearly defined. In this study, using various techniques such as high throughput sequencing technology, bioinformatics, quantitative real-time PCR, and immunoblot analysis,we aimed to understand whether matrine serves as a novel regulator of FOXO and PI3K-AKT signaling pathway. DU145 and PC-3 cell lines were cultured for 24 h in vitro. Cells were treated with either matrine or control serum for 48 h, followed by extraction of total RNA. The RNA was sequenced using HiSeq 2500 high-throughput sequencing platform (Illumina). A gene library was established and quality analysis of read data carried out. Integrated database from the website DAVID was used to analyze Gene Ontology (GO), and Kyoto encyclopedia of genes and genomes (KEGG) pathway of differential genes was used for pathway analysis, screening for fold differences of more than two times. The FOXO and PI3K-AKT signaling pathways were screened, and expression levels of mRNA and core protein detected by real-time PCR and immunoblotting, respectively. High throughput sequencing and GO analysis revealed that differentially expressed genes before and after treatment played an important role in cell metabolic process, growth process, anatomical structure formation, cellular component organization, and biological regulation. KEGG signal pathway analysis revealed that FOXO and PI3K-AKT signal pathways had a significant difference between before and after matrine-treated androgen-independent prostate cancer cells PC-3 and DU145. Real-time PCR showed that matrine treatment led to a significant increase in the expression levels of FOXO1A, FOXO3A, FOXO4, and FOXO6 in DU145 and PC-3 cells (P<0.01 or P<0.05), whereas the PI3K expression levels decreased (P<0.01). Similarly, immunoblotting revealed a significant increase (P<0.05) in the expression levels of FOXO1A FOXO3A, FOXO4, and FOXO6 in both PC-3 and DU145 cells, whereas PI3K expression levels decreased (P<0.05). Matrine had a broad regulating effect on the mRNA expression profiles of both PC-3 and DU145 cells. Matrine may inhibit cell proliferation, migration, as well as invasion, and induce apoptosis in both PC-3 and DU145 cells through FOXO and PI3K-AKT signaling pathways. Matrine could therefore be used as a complementary drug to present chemotherapeutic agents, for treating androgen-independent prostate cancer. | Qi Li HaiH uang Zheng He Yi Sun Yufeng Tang Xiaohong Shang Chengbin Wang | 2018 | Science China(Life Sciences)2018，61，5： | 9 |
| 16 | Characterization and Fine Mapping of a Novel Rice Albino Mutant low temperature albino 1显示文摘白化体异种是为学习叶绿素生合成和叶绿体开发并且克隆在植物涉及这些过程的基因的有用基因资源。这里,我们报导新奇米饭异种低温度白化体 1 (lta1 ) 当在比 20 楢潮低的温度下面成长时,那显示出的白化体在 4 | Yu Peng Yi Zhang Jun Lv Jianhui Zhang Ping Li Xiaoliang Shi Yufeng Wang Honglei Zhang Zuhua He Sheng Teng | 2012 | Journal of Genetics and Genomics2012，39，8： | 9 |
| 17 | Three-dimensional-printed individualized porous implants:A new“implant-bone”interface fusion concept for large bone defect treatment显示文摘Bone defect repairs are based on bone graft fusion or replacement.Current large bone defect treatments are inadequate and lack of reliable technology.Therefore,we aimed to investigate a simple technique using three-dimensional(3D)-printed individualized porous implants without any bone grafts,osteoinductive agents,or surface biofunctionalization to treat large bone defects,and systematically study its long-term therapeutic effects and osseointegration characteristics.Twenty-six patients with large bone defects caused by tumor,infection,or trauma received treatment with individualized porous implants;among them,three typical cases underwent a detailed study.Additionally,a large segmental femur defect sheep model was used to study the osseointegration characteristics.Immediate and long-term biomechanical stability was achieved,and the animal study revealed that the bone grew into the pores with gradual remodeling,resulting in a long-term mechanically stable implant-bone complex.Advantages of 3D-printed microporous implants for the repair of bone defects included 1)that the stabilization devices were immediately designed and constructed to achieve early postoperative mobility,and 2)that osseointegration between the host bone and implants was achieved without bone grafting.Our osseointegration method,in which the“implant-bone”interface fusion concept was used instead of“bone-bone”fusion,subverts the traditional idea of osseointegration. | Teng Zhang Qingguang Wei Hua Zhou Zehao Jing Xiaoguang Liu Yufeng Zheng Hong Cai Feng Wei Liang Jiang Miao Yu Yan Cheng Daoyang Fan Wenhao Zhou Xinhong Lin Huijie Leng Jian Li Xinyu Li Caimei Wang Yun Tian Zhongjun Liu | 2021 | Bioactive Materials2021，6，11： | 9 |
| 18 | Optimizing mechanical property and cytocompatibility of the biodegradable Mg-Zn-Y-Nd alloy by hot extrusion and heat treatment显示文摘The Mg-Zn-Y-Nd alloy is a new type of degradable material for biomedical application. In the present study, Mg-6Zn-1.2Y-0.8Nd alloy was fabricated, and then extrusion and heat treatment were conducted to optimize its mechanical properties and cytocompatibility. The microstructure observation, mechanical property, degradation behavior and cytocompatibility tests were conducted on the Mg-Zn-Y-Nd alloy with three different states: as-cast(alloy C), as-extruded(alloy E) and extruded + heat treated(alloy EH).The results show that alloy C consists of coarse grains and continuous secondary phases. The extrusion process has caused incomplete recrystallization, and results in a mixed grain structure of elongated grains and small equiaxed grains(alloy E). The heat treatment process has promoted the recrystallization and homogenized the grain structure(alloy EH). Both the strength and ductility of the alloy has been improved by extrusion, but the following heat treatment has decreased the strength and increased the ductility.The degradation behavior of the alloy C and E alloys does not show much difference, but improves slightly in alloy EH, because the heat treatment has homogenized the microstructure and released the residual stress in the alloy. The directly and indirectly cell viability tests indicate that alloy EH exhibits the best cytocompatibility, which should be ascribed to its relative uniform degradation and low ion releasing rate. In summary, the combination of hot extrusion and heat treatment could optimize the mechanical property and cytocompatibility of the Mg-Zn-Y-Nd alloy together, which is beneficial for the future application of the alloy. | Yiyuan Kang Beining Du Yueming Li Baojie Wang Liyuan Sheng Longquan Shao Yufeng Zheng Tingfei Xi | 2019 | Journal of Materials Science & Technology2019，35，1： | 8 |
| 19 | CRISPR-Cas13a mediated nanosystem for attomolar detection of canine parvovirus type 2显示文摘Canine parvovirus type 2(CPV-2) infection is the most lethal disease of dogs with higher mortality in puppies worldwide.In today’s world,dogs are an integral part of our communities as well as dogs breeding and rearing has become a lucrative business.Therefore,a fast,accurate,portable,and costeffective CPV-2 detection method with the ability for on-site detection is highly desired.In this study,we for the first time proposed a nanosystem for CPV-2 DNA detection with RNA-guided RNA endonuclease Cas13 a,which upon activation results in collateral RNA degradation.We expressed LwCasl3 a in prokaryotic expression system and purified it through nickel column.Activity of Cas13 a was verified by RNA-bound fluorescent group while using a quenched fluorescent probe as signals.Further Cas13 a was combined with Recombinase polymerase amplification(RPA) and T7 transcription to establish molecular detection system termed specific high-sensitivity enzymatic reporter un-locking(SHERLOCK) for sensitive detection of CPV-2 DNA.This nanosystem can detect 100 amol/L CPV-2 DNA within 30 min.The proposed nanosystem exhibited high specificity when tested for CPV-2 and other dog viruses.This CRISPR-Cas13 a mediated sensitive detection approach can be of formidable advantage during CPV-2 outbreaks because it is time-efficient,less laborious and does not involve the use of sophisticated instruments. | Haroon Khan Adeel Khan Yufeng Liu Su Wang Sumaira Bibi Hongpan Xu Yuan Liu Samran Durrani Lian Jin Nongyue He Tao Xiong | 2019 | Chinese Chemical Letters2019，30，12： | 8 |
| 20 | In vitro degradation and biocompatibility evaluation of typical biodegradable metals (Mg/Zn/Fe) for the application of tracheobronchial stenosis显示文摘Tracheobronchial obstruction in children due to benign stenosis or tracheobronchomalacia still remains a challenging matter of concern.Currently,there is 10%–20%complication rate in clinical treatment.The nonbiodegradable property of silicone stents and nickel-titanium memory alloy stents take the primary responsibility for drawbacks including stimulating local granulation tissue proliferation,displacement,and stent-related infections.Permanent tracheobronchial stent will be a persistent foreign object for a long time,causing excessive secretion of tracheal mucosa,ulceration and even perforation,which is particularly unsuitable for young children with persistent tracheal growth.In this study,the degradation and biocompatibility performance of three typical biodegradable metals were investigated as potential tracheobronchial stent materials.The results exhibited that these materials showed different degradation behaviors in the simulating respiratory fluid environment compared with SBF.Except for pure iron group,high purity magnesium and zinc showed favorable cell adhesion and proliferation in three culture methodologies(direct culture,indirect culture and extraction culture).The proper corrosion rate and good biocompatibility indicated that high purity magnesium and zinc may be good candidates as tracheobronchial stent materials. | Yangyang Li Jianglong Yan Wenhao Zhou Pan Xiong Pei Wang Wei Yuan Yufeng Zheng Yan Cheng | 2019 | Bioactive Materials2019，4，1： | 8 |
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