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| 1 | CRISPR/Cas9-mediated gene editing in human tripronuclear zygotes显示文摘染色体编辑工具例如定期聚类短 palindromic 重复(CRISPR ) 联系了的 interspaced 系统(Cas ) 广泛地被用来包括动物接合子和人的房间在模型系统修改基因,并且为基本研究和临床的应用保持巨大的诺言。迄今为止,严肃的知识差距在人的早胚胎,并且在效率和在人的培植前胚胎使用象 CRISPR/Cas9 那样的技术的潜在的离开目标效果留在我们 DNA 修理机制的理解。在这份报告,我们使用了推进的接合子调查 CRISPR/Cas9-mediated 基因在人的房间编辑的 tripronuclear (3PN ) 。我们发现 CRISPR/Cas9 能有效地劈开内长的 -globin 基因(HBB ) 。然而,相应再结合的效率指导了 HBB 的修理(HDR ) 是低的,编辑胚胎是马赛克。离开目标劈开在由 T7E1 试金并且 whole-exome 定序揭示了的这些 3PN 接合子也是明显的。而且,内长的 delta-globin 基因(HBD ) 对 HBB 相应,与外长的施主 oligos 竞争了充当修理模板,导致倔强的变化。我们的数据也显示在这些胚胎的 HBB 地点的修理通过非转线路 HDR 小径优先地发生了。一起拿,我们的工作加亮紧迫的需要进一步改进 CRISPR/Cas9 平台的忠实和特性,为编辑的 CRSIPR/Cas9-mediated 的任何临床的应用程序的一个前提。 | Puping Liang Yanwen Xu Xiya Zhang Chenhui Ding Rui Huang Zhen Zhang Jie Lv Xiaowei Xie Yuxi Chen Yujing Li Ying Sun Yaofu Bai Zhou Songyang Wenbin Ma Canquan Zhou Junjiu Huang | 2015 | Protein & Cell2015，6，5： | 149 |
| 2 | Tissue-specific transcription reprogramming promotes liver metastasis of colorectal cancer显示文摘Metastasis,the development of secondary malignant growths at a distance from a primary tumor,is the cause of death for 90%of cancer patients,but little is known about how metastatic cancer cells adapt to and colonize new tissue environments.Here,using clinical samples,patient-derived xenograft(PDX)samples,PDX cells,and primary/metastatic cell lines,we discovered that liver metastatic colorectal cancer(CRC)cells lose their colon-specific gene transcription program yet gain a liver-specific gene transcription program.We showed that this transcription reprogramming is driven by a reshaped epigenetic landscape of both typical enhancers and super-enhancers.Further,we identified that the liver-specific transcription factors FOXA2 and HNF1A can bind to the gained enhancers and activate the liver-specific gene transcription,thereby driving CRC liver metastasis.Importantly,similar transcription reprogramming can be observed in multiple cancer types.Our data suggest that reprogrammed tissue-specific transcription promotes metastasis and should be targeted therapeutically. | Shuaishuai Teng Yang Eric Li Ming Yang Rui Qi Yiming Huang Qianyu Wang Yanmei Zhang Shanwen Chen Shasha Li Kequan Lin Yang Cao Qunsheng Ji Qingyang Gu Yujing Cheng Zai Chang Wei Guo Pengyuan Wang Ivan Garcia-Bassets Zhi John Lu Dong Wang | 2020 | Cell Research2020，30，1： | 11 |
| 3 | Alterations in gut microbiota and metabolites associated with altitude-induced cardiac hypertrophy in rats during hypobaric hypoxia challenge显示文摘The gut microbiota is involved in host responses to high altitude.However,the dynamics of intestinal microecology and their association with altitude-related illness are poorly understood.Here,we used a rat model of hypobaric hypoxia challenge to mimic plateau exposure and monitored the gut microbiome,short-chain fatty acids(SCFAs),and bile acids(BAs)over 28 d.We identified weight loss,polycythemia,and pathological cardiac hypertrophy in hypoxic rats,accompanied by a large compositional shift in the gut microbiota,which is mainly driven by the bacterial families of Prevotellaceae,Porphyromonadaceae,and Streptococcaceae.The aberrant gut microbiota was characterized by increased abundance of the Parabacteroides,Alistipes,and Lactococcus genera and a larger Bacteroides to Prevotella ratio.Trans-omics analyses showed that the gut microbiome was significantly correlated with the metabolic abnormalities of SCFAs and BAs in feces,suggesting an interaction network remodeling of the microbiome-metabolome after the hypobaric hypoxia challenge.Interestingly,the transplantation of fecal microbiota significantly increased the diversity of the gut microbiota,partially inhibited the increased abundance of the Bacteroides and Alistipes genera,restored the decrease of plasma propionate,and moderately ameliorated cardiac hypertrophy in hypoxic rats.Our results provide an insight into the longitudinal changes in intestinal microecology during the hypobaric hypoxia challenge.Abnormalities in the gut microbiota and microbial metabolites contribute to the development of high-altitude heart disease in rats. | Zhiyuan Pan Yichen Hu Zongyu Huang Ni Han Yan Li Xiaomei Zhuang Jiye Yin Hui Peng Quansheng Gao Wenpeng Zhang Yong Huang Yujun Cui Yujing Bi Zhenjiang Zech Xu Ruifu Yang | 2022 | Science China(Life Sciences)2022，65，10： | 5 |
| 4 | PtxCuy nanocrystals with hexa-pod morphology and their electrocatalytic performances towards oxygen reduction reaction显示文摘二金属的磅 x 有明确的昆虫形态学的 Cu y nanocrystals (NC ) 经由一条湿化学途径被综合。有约 20 nm 表演的尺寸的同样综合的凸的 NC 暴露了低索引(111 ) 种子上的方面和豆荚上的各种各样的高索引的方面。生长机制沿着 cuboctahedral 上的结晶的方向播种的 <100> 包含了比较喜欢的生长。合成协议能被用于磅 x 有各种各样的 Cu/Pt 比率的 Cu y NC。向氧减小反应(ORR ) 在碳黑色上支持的昆虫 PtxCuy NC 的电镀物品催化的活动被学习。昆虫 PtCu 2/C 催化剂展出最高特定的活动(3.7 妈 / 厘米 磅 2) 和集体活动(2.4 A/mg 磅 ) 报导了为磅 x Cu y 。有磅 x Cu y 显示提高的活动从词法因素发源。豆荚上的高索引的方面以及丰富的边和步的存在能相当解释提高的催化活动。昆虫磅 x Cu y /C 催化剂也在加速的耐久性测试以后显示出高词法的稳定性和活动。同样综合的昆虫磅 x Cu y NC 为质子交换膜燃料房间作为阴极电镀物品催化剂有高潜力。 | Yujing Li Fanxin Quan Enbo Zhu Lin Chen Yu Huang Changfeng Chen | 2015 | Nano Research2015，8，10： | 5 |
| 5 | Differential transcriptome analysis between Populus and its synthesized allotriploids driven by second-division restitution显示文摘In this report, we compared transcriptomic differences between a synthetic Populus section Tacamahaca triploid driven by second-division restitution and its parents using a high-throughput RNA-seq method. A total of 4,080 genes were differentially expressed between the high-growth vigor allotriploids(SDR-H) and their parents, and 719 genes were non-additively expressed in SDR-H. Differences in gene expression between the allotriploid and male parent were more signi ficant than those between the allotriploid and female parent, which may be caused by maternal effects. We observed3,559 differentially expressed genes(DEGs) between the SDR-H and male parent. Notably, the genes were mainly involved in metabolic process, cell proliferation, DNA methylation, cell division, and meristem and developmental growth. Among the1,056 DEGs between SDR-H and female parent, many genes were associated with metabolic process and carbon utilization.In addition, 1,789 DEGs between high- and low-growth vigor allotriploid were mainly associated with metabolic process,auxin poplar transport, and regulation of meristem growth.Our results indicated that the higher poplar ploidy level can generate extensive transcriptomic diversity compared with its parents. Overall, these results increased our understanding of the driving force for phenotypic variation and adaptation in allopolyploids driven by second-division restitution. | Shiping Cheng Zhen Huang Yun Li Ting Liao Yujing Suo Pingdong Zhang Jun Wang Xiangyang Kang | 2015 | Journal of Integrative Plant Biology2015，57，12： | 3 |
| 6 | Enhancement of oxygen reduction reaction activity by grain boundaries in platinum nanostructures显示文摘Systematic control of grain boundary densities in various platinum(Pt)nanostructures was achieved by specific peptide-assisted assembly and coagulation of nanocrystals.A positive quadratic correlation was observed between the oxygen reduction reaction(ORR)specific activities of the Pt nanostructures and the grain boundary densities on their surfaces.Compared to commercial Pt/C,the grain-boundary-rich strain-free Pt ultrathin nanoplates demonstrated a 15.5 times higher specific activity and a 13.7 times higher mass activity.Simulation studies suggested that the specific activity of ORR was proportional to the resident number and the resident time of oxygen on the catalyst surface,both of which correlate positively with grain boundary density,leading to improved ORR activities. | Enbo Zhu Wang Xue Shiyi Wang Xucheng Yan Jingxuan Zhou Yang Liu Jin Cai Ershuai Liu Qingying Jia Xiangfeng Duan Yujing Li Hendrik Heinz Yu Huang | 2020 | Nano Research2020，13，12： | 3 |
| 7 | Do trace metal(loid)s in road soils pose health risks to tourists?A case of a highly-visited national park in China显示文摘Nowadays,more people tend to spend their recreational time in large national parks,and trace metal(loid)s in soils have attracted long-term attention due to their possible harm to human health.To investigate the pollution levels,potential sources and health risks of trace metal(loid)s in road soils,a total of eight trace metal(loid)s(including As,Cd,Cr,Cu,Ni,Pb,Zn and Hg)from 47 soil samples along roads were studied in the Huangshan National Park in Southeast China.The results showed that the concentrations of As,Cd,Pb,Zn and Hg appeared different degrees of pollution compared with their corresponding background values.According to the pollution indices,Hg and Cd were recognized as significant pollutants presenting moderate to high ecological risk.Combining principal component analysis and positive matrix factorization model,the results showed that traffic,industrial,agricultural and natural sources were the potential origins of trace metal(loid)s in this area,with contribution rates of 39.93%,25.92%,10.53%and 23.62%,respectively.Non-carcinogenic risks were all negligible,while the carcinogenic risk of As was higher than the limit(1×10^(-6)).Moreover,children were more susceptible to trace metal(loid)s by ingestion which appeared to be a more important exposure pathway than dermal contact and inhalation.The contribution rates of different sources to non-carcinogenic risks and carcinogenic risks were similar among children and adults,while traffic and industrial sources have a significant impact on health risks.This study will give more insights to control the environmental risks of trace metal(loid)s in national parks. | Jingling Huang Yuying Wu Yanyao Li Jiaxun Sun Yujing Xie Zhengqiu Fan | 2022 | Journal of Environmental Sciences2022，34，1： | 2 |
| 8 | Nitric oxides and nitrous oxide fluxes from typical vegetables cropland in China: Effects of canopy, soil properties and field management显示文摘 | Xiaobing Pang Yujing Mu Xinqing Lee Shuangxi Fang Juan Yuan Daikuan Huang | 2009 | Atmospheric Environment2009，，16： | 2 |
| 9 | Geochemistry, zircon U–Pb ages and Lu–Hf isotopes of early Paleozoic plutons in the northwestern Chinese Tianshan: Petrogenesis and geological implications显示文摘 | Zongying Huang Xiaoping Long Alfred Kr?ner Chao Yuan Qiang Wang Min Sun Guochun Zhao Yujing Wang | 2013 | LITHOS2013，，： | 1 |
| 10 | Human cytomegalovirus miR-US5-1 inhibits viral replication by targeting Geminin mRNA显示文摘Viruses commonly create favorable cellular conditions for their survival through multiple mechanisms. Micro RNAs(mi RNAs), which function as post-transcriptional regulators, are utilized by human cytomegalovirus(HCMV) in its infection and pathogenesis. In the present study, the DNA replication inhibitor Geminin(GMNN) was identified to be a direct target of hcmv-mi R-US5-1. Overexpression of hcmv-mi R-US5-1 could block the accumulation of GMNN during HCMV infection, and the decrease of GMNN expression caused by hcmv-mi R-US5-1 or GMNN specific si RNA reduced HCMV DNA copies in U373 cells. Meanwhile, ectopic expression of hcmv-mi R-US5-1 and consequent lower expression of GMNN influenced host cell cycle and proliferation. These results imply that hcmv-mi R-US5-1 may affect viral replication and host cellular environment by regulating expression kinetics of GMNN during HCMV infection. | Shujuan Jiang Yujing Huang Ying Qi Rong He Zhongyang Liu Yanping Ma Xin Guo Yaozhong Shao Zhengrong Sun Qiang Ruan | 2017 | Virologica Sinica2017，32，5： | 1 |
| 11 | Efficient silencing of the multicopy DUX4 gene by ABE-mediated start codon mutation in human embryos显示文摘The Dux gene plays an important role in mouse embryo development(De Iaco et al.,2017;Hendrickson et al.,2017;Whiddon et al.,2017).It participates in the maternal-to-zygotic transition(MTZ),which mainly includes maternal transcript degradation and zygotic genome activation(ZGA)and is the key process for embryonic development.Approximately 11-100 copies of the DUX4 gene form a tandem cluster in the human genome(Snider et al.,2009,2010). | Yubing Liu Xinmei Lu Min Ye Ling Wang Rongxin Tang Zhiyong Yang Bongkoch Turathum Chenchen Liu Yujing Xue Menghua Wu Yannan Yang Ermeng Gao Di Zhang Feng Yang Keh-Kooi Kee Xingxu Huang Guanglei Li Ri-Cheng Chian | 2022 | Journal of Genetics and Genomics2022，49，10： | 1 |
| 12 | Efficient catalytic hydrogen generation by intermetallic platinum-lead nanostructures with highly tunable porous feature显示文摘The water-gas shift(WGS) reaction is an essential industrial reaction for upgrading hydrogen(H2) by removing carbon monoxide(CO), while highly efficient platinum(Pt)-based catalysts for WGS with simultaneously high activity and stability are still yet to be developed due to the poisoning issue during the reaction. Herein, we report on the porous PtPb peanut nanocrystals(porous PtPb PNCs) and porous PtPb octahedron nanocrystals(porous PtPb ONCs) with controllable ratios of Pt/Pb as extremely active and stable catalysts towards WGS reaction. It exhibits the composition-dependent activity with porous PtPb PNCs-40/ZnO being the most active for WGS to H_2, 16.9 times higher than that of the commercial Pt/C. The porous PtPb PNCs-40/ZnO also display outstanding durability with barely activity decay and negligible structure and composition changes after ten successive reaction cycles. X-ray photoelectron spectroscopy(XPS) results reveal that the suitable binding energy of Pt 4f_(7/2) and the high ratio of Pt(0)to Pt(II) in porous PtPb PNCs/ZnO and porous PtPb ONCs/ZnO are crucial for the enhanced WGS activity.The CO stripping results indicate the optimized CO adsorption strength on the Pt surface ensure the excellent WGS activity and the outstanding durability. The present work demonstrates an important advance in tuning the porous metal nanomaterials as highly efficient and durable catalysts for catalysis,energy conversion and beyond. | Bin E Lingzheng Bu Qi Shao Yujing Li Xiaoqing Huang | 2019 | Science Bulletin2019，64，1： | 1 |
| 13 | Red and processed meat intake and risk of esophageal adenocarcinoma: a meta-analysis of observational studies显示文摘 | Wen Huang Yujing Han Jiping Xu Wei Zhu Zhaoshen Li | 2013 | Cancer Causes & Control2013，，1： | 1 |
| 14 | HbA1c and the diagnosis of diabetes and prediabetes in a middle‐aged and elderly H an population from northwest C hina (HbA1c与中国西北部地区中老年汉族人群糖尿病和糖尿病前期的诊断)显示文摘 | Shengli Wu Fengshou Yi Chao Zhou Mintao Zhang Yujing Zhu Yilihamu Tuniyazi Lijuan Huang Xuefang Huang Fugang Wang Yufang Bi Guang Ning | 2013 | Journal of Diabetes2013，，： | 1 |
| 15 | Red and processed meat intake and risk of esophageal adenocarcinoma:a meta-analysis of observational studies显示文摘 | HUANG Wen HAN Yujing XU Jiping | 2013 | Cancer Causes Control2013，24，： | 1 |
| 16 | HbAlc and the diagnosis of diabetes and prediabetes in a middle-aged and elderly Han population from northwest China (I-lbA1 )显示文摘 | Shengli Wu Fengshou i Chao Zhou Mintao Zhang Yujing Zhu Yilihamu Tuniyazi Lijuan Huang Xuefang Huang Fugang Wang Yufang Bi Guang Ning | 2013 | Journal of diabetes2013，5，3： | 1 |
| 17 | Synthesis of Subnanometer-Sized Gold Clusters by a Simple Milling- Mediated Solid Reduction Method显示文摘 | Qinggang Liu Xinkui Wang Yujing Ren Xiaofeng Yang Zhilian Wu Xiaoyan Liu Lin Li Shu Miao Yang Su Yanqin Li Changhai Liang Yanqiang Huang | 2018 | Chinese Journal of Chemistry2018，36，4： | 1 |
| 18 | Functionalizingnano-montmorillonites by modified with intumescent flameretardant:Preparation and application in polyurethane显示文摘 | Huang Guobo Gao Jianrong Li Yujing | 2010 | Polymer Degradationand Stability2010，95，： | 1 |
| 19 | Dao-Tan decoction inhibits tumor necrosis factor-α-induced intercellular adhesion molecule-1 expression by blocking JNK and p38 signaling pathways in human umbilical vein endothelial cells显示文摘 | Xiaobo Huang Fen Wang Wenqiang Chen Zongxin Li Ningqun Wang Yujing Chen Kristine von Maltzan | 2012 | Pharmaceutical Biology2012，，9： | 1 |
| 20 | Func- tionalizing Nano-montmorillonites by Modified with In- tumescent Flame Retardant: Preparation and Applica- tion in Polyurethane 显示文摘 | Huang Guobo Gao Jianrong Li Yujing | 2010 | Polymer Degradation and Stability2010，95，2： | 1 |
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