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| 1 | Translocation of mixed lineage kinase domain-like protein to plasma membrane leads to necrotic cell death显示文摘混合的系 kinase 像域的蛋白质(MLKL ) 被识别受体交往下游地工作蛋白质 3 (RIP3 ) 在肿瘤坏死 factor-α(TNF ) 导致了坏死(也叫的 necroptosis ) 。然而, MLKL 怎么工作调停 necroptosis 是未知的。由在 MLKL 大美人房间的 MLKL 功能的宪法,我们证明 MLKL 的 N 终点在 necroptosis 为它的功能被要求。在对待 TNF 的房间的 MLKL 的 oligomerization 为 necroptosis 是必要的,当人工地由使用荷尔蒙绑定领域一起强迫 MLKL (HBD*) 扳机 necroptosis。尤其是,一起强迫 N 终端领域(ND ) 然而并非 MLKL 的 C 终端 kinase 领域引起 necroptosis。进一步的删除分析证明 four-α MLKL (1-130 氨基酸) 的螺旋捆是足够的触发 necroptosis。两 HBD*调停并且 MLKL (ND ) 或 MLKL 的导致 TNF 的建筑群是到血浆膜的类脂化合物椽子的这些建筑群的 tetramers,和 translocation 先于房间死亡。homo-oligomerization 为 MLKL translocation 被要求,为血浆膜地点的信号顺序位于第一和第二 α 的连接; MLKL 的 -helices。MLKL 或 MLKL (ND ) 的血浆膜 translocation 导致钠流入,并且从房间文化媒介的钠的弄空禁止 necroptosis。上述所有现象没在 apoptosis 被看见。因此, MLKL oligomerization 导致 MLKL 的 translocation 到血浆膜,和血浆膜的类脂化合物椽子由自己或经由到增加的另外的蛋白质的 MLKL 复杂行为任何一个钠流入,增加渗透的压力,最后导致膜破裂。 | Xin Chen Wenjuan Li Junming Ren Deli Huang Wan-ting He Yunlong Song Chao Yang Wanyun Li Xinru Zheng PengdaChen Jiahuai Han | 2014 | Cell Research2014，24，1： | 67 |
| 2 | Bioaugmentation of DDT-contaminated soil by dissemination of the catabolic plasmid pDOD显示文摘A plasmid transfer-mediated bioaugmentation method for the enhancement of dichlorodiphenyltrichloroethane(DDT) degradation in soil was developed using the catabolic plasmid p DOD from Sphingobacterium sp. D-6. The p DOD plasmid could be transferred to soil bacteria, such as members of Cellulomonas, to form DDT degraders and thus accelerate DDT degradation. The transfer efficiency of p DOD was affected by the donor, temperature,moisture, and soil type. Approximately 50.7% of the DDT in the contaminated field was removed 210 days after the application of Escherichia coli TG I(p DOD-gfp). The results suggested that seeding p DOD into soil is an effective bioaugmentation method for enhancing the degradation of DDT. | Chunming Gao Xiangxiang Jin Jingbei Ren Hua Fang Yunlong Yu | 2015 | Journal of Environmental Sciences2015，27，1： | 6 |
| 3 | Stretchable supercapacitor based on a hierarchical PPy/CNT electrode and hybrid hydrogel electrolyte with a wide operating temperature显示文摘Hydrogel is frequently used as a solid electrolyte for all solid-state supercapacitors(SCs)because of its liquid-like ion-transport property and high conformability.However,due to the higher water content,the hydrogel electrolyte undergoes inevitable freezing and/or dehydration with climate change.Herein,polypyrrole/carbon all-solid-state SCs(PCSCs)were developed based on a hierarchical polypyrrole/carbon nanotube electrode and a highly stretchable double-network polymer hydrogel electrolyte with LiCl/ethylene glycol as a mixed solvent.The PCSCs showed excellent electrochemical performance and cycle stability with a wide operating temperature.The specific capacitances could reach 202.2 and 112.3 mF cm^(−2) at current densities of 0.5 and 3.0 mA cm^(−2),respectively.Meanwhile,the PCSCs showed outstanding mechanical properties in maintaining a high areal capacitance under deformations of bending and tension.The excellent water retention of the device also ensured the stable electrochemical performance of PCSCs in a wide temperature range(30–80℃),which could potentially represent a reliable application in various harsh environments. | Yanfang Ren Yunlong Liu Siying Wang Qian Wang Shuhong Li Wenjun Wang Xiaochen Dong | 2022 | Carbon Energy2022，4，4： | 2 |
| 4 | Leakage current characteristics and ageing assessment technology of roof arrester under ultra harmonics overvoltage显示文摘The large leakage current of the roof arrester caused by the high‐frequency resonant overvoltage on the high‐speed train occurs intermittently,which will cause the arrester to overheat locally.In this work,the overheating issue of the arrester under Ultra Harmonics Overvoltage is studied.Then,the relationship between the active power and the voltage(including amplitude and frequency)of the arrester is investigated,and the impact mechanism of the harmonic voltage on the overheating fault of the arrester is analysed based on experiments in this work.It is found that the total leakage current of the arrester is proportional to the amplitude and frequency of the applied voltage.The internal resistance of the arrester is independent of the amplitude of the voltage,but it will decrease with the increase of the frequency.The main reason that leads to thermal breakdown of the arrester valve under high‐frequency harmonic voltage is the heating power elevation of the arrester valve.The research study can provide theoretical basis and data support for the design,type selection and monitoring of the high‐speed train roof arrester. | Jixing Sun Fan Ding Yunlong Lv Jiayi Ren Sibo Song Tiance Li Qingyun Zhi Changwang Guo | 2022 | High Voltage2022，7，2： | 1 |
| 5 | Ground simulation of flow and heat transfer under micro-gravity显示文摘 | Xingang Liang Zengyuan Guo Yunlong Liu Jianxun Ren | 1997 | Science in China Series A: Mathematics1997，，11： | 1 |
| 6 | VEGF-B prevents excessive angiogenesis by inhibiting FGF2/FGFR1 pathway显示文摘Although VEGF-B was discovered as a VEGF-A homolog a long time ago,the angiogenic effect of VEGF-B remains poorly understood with limited and diverse findings from different groups.Notwithstanding,drugs that inhibit VEGF-B together with other VEGF family members are being used to treat patients with various neovascular diseases.It is therefore critical to have a better understanding of the angiogenic effect of VEGF-B and the underlying mechanisms.Using comprehensive in vitro and in vivo methods and models,we reveal here for the first time an unexpected and surprising function of VEGF-B as an endogenous inhibitor of angiogenesis by inhibiting the FGF2/FGFR1 pathway when the latter is abundantly expressed.Mechanistically,we unveil that VEGF-B binds to FGFR1,induces FGFR1/VEGFR1 complex formation,and suppresses FGF2-induced Erk activation,and inhibits FGF2-driven angiogenesis and tumor growth.Our work uncovers a previously unrecognized novel function of VEGF-B in tethering the FGF2/FGFR1 pathway.Given the anti-angiogenic nature of VEGF-B under conditions of high FGF2/FGFR1 levels,caution is warranted when modulating VEGF-B activity to treat neovascular diseases. | Chunsik Lee Rongyuan Chen Guangli Sun Xialin Liu Xianchai Lin Chang He Liying Xing Lixian Liu Lasse DJensen Anil Kumar Harald FLanger Xiangrong Ren Jianing Zhang Lijuan Huang Xiangke Yin JongKyong Kim Juanhua Zhu Guanqun Huang Jiani Li Weiwei Lu Wei Chen Juanxi Liu Jiaxin Hu Qihang Sun Weisi Lu Lekun Fang Shasha Wang Haiqing Kuang Yihan Zhang Geng Tian Jia Mi Bi-Ang Kang Masashi Narazaki Aaron Prodeus Luc Schoonjans David MOrnitz Jean Gariepy Guy Eelen Mieke Dewerchin Yunlong Yang Jing-Song Ou Antonio Mora Jin Yao Chen Zhao Yizhi Liu Peter Carmeliet Yihai Cao Xuri Li | 2023 | Signal Transduction and Targeted Therapy2023，8，9： | 0 |
| 7 | Towards growth of pure AB-stacked bilayer graphene single crystals显示文摘Given its intriguing band structure and unique tunable bandgap,AB-stacked bilayer graphene has great potentials in the applications of high-end electronics,optoelectronics and semiconductors.The epitaxial growth of AB-stacked single-crystal bilayer graphene films requires a strict AB-stacked lattice,identical orientations and seamless stitching of bilayer graphene islands.However,the particles inevitably present on the metal surface that produced during high temperature growth would induce random orientations,twisted stacking islands,and uncontrollable multilayers,which is a great challenge to overcome.Here,we propose a heat-resisting-box assisted strategy to produce nearly pure AB-stacked bilayer graphene single-crystal films on Cu/Ni(111)foils.With our technique,the particles on the Cu/Ni(111)surface are effectively eliminated,which greatly minimizes the occurrence of randomly twisted islands and uncontrollable multilayers.The as-grown AB-stacked bilayer graphene films show>99%alignment and>99%AB stacking order.Our work provides a promising method towards the growth of pure AB-stacked bilayer graphene single crystals and would accelerate its device applications. | Xiaowen Zhang Tao Zhou Yunlong Ren Zuo Feng Ruixi Qiao Qinghe Wang Bin Wang Jinxia Bai Muhong Wu Zhilie Tang Xu Zhou Kaihui Liu Xiaozhi Xu | 2024 | Nano Research2024，17，5： | 0 |
| 8 | Targeted combination therapy for glioblastoma by co-delivery of doxorubicin,YAP-siRNA and gold nanorods显示文摘The combination of brain targeting drug delivery systems and multi-modal intervention pose a promising therapeutic approach for glioblastoma therapy.In this study,we developed an angiopep-2 peptide modified cationic liposome loaded with doxorubicin,YAP-siRNA and gold nanorods(D/R@Ang2-Lip+Au)simultaneously,which has high encapsulating efficiency for doxorubicin(95.4%)and effective binding of siRNA at N/P ratio of 20:1.The fluorescence imaging and flow cytometry analysis revealed high cellular uptake of D/R@Ang2-Lip+Au.Real-time quantitative polymerase chain reaction and western blot analysis indicated that D/R@Ang2-Lip+Au could effectively inhibit the expression of YAP protein.In vitro and in vivo studies showed that D/R@Ang2-Lip+Au had the ability to target glioblastoma cells,and achieved better anti-proliferative effects compared with non-targeted D/R@Lip+Au.Moreover,in vivo experiment demonstrated that D/R@Ang2-Lip+Au was able to cross the blood-brain barrier,and combination therapy could significantly inhibit tumor growth.Therefore,the multifunctional D/R@Ang2-Lip+Au might provide a novel approach for effectively delivery of DOX,YAP-siRNA and AuNRs into the glioblastoma cells simultaneously and exerting synergistic therapeutic effects. | Lihuang Li Qiuyan Guo Yanxiu Liu Mindan Lu Jun Yang Yunlong Ge Qiang Zhang Benqiang Sun Xiumin Wang Li Liang-cheng Lei Ren | 2021 | Journal of Materials Science & Technology2021，，4： | 0 |
| 9 | 氮添加对内蒙古草原植物地上和地下碳氮磷化学计量学特征的影响显示文摘氮沉降对生态系统功能和过程具有显著影响。已有研究表明氮添加会影响植物叶片C:N:P化学计量特征。然而,氮添加对植物根系C:N:P化学计量特征影响的研究罕有报道。本论文的主要目的是对比氮添加对草原植物群落地上和地下C:N:P化学计量特征的影响。我们在内蒙古锡林郭勒草原开展了一个7年的氮添加实验,该实验包括6个处理,分别是对照:control;N1:0.4 mol·m-2 N;N2:0.8 mol·m^(-2) N;N3:1.6 mol·m^(-2) N;N4:2.8 mol·m^(-2) N;N5:4 mol·m^(-2) N,每个处理6次重复。我们测量并分析了植物地上、地下生物量和C:N:P化学计量特征。研究结果表明,氮添加会导致植物群落地上部分碳浓度降低,氮浓度和磷浓度升高,C:N和C:P比值降低,N:P升高,而且地上碳、氮、磷库均因施氮而增加。然而,氮添加对0-10,10-30,30-50和50-100 cm 4个土层的地下部分碳、氮、磷浓度、比例、库和化学计量特征没有显著影响。综上所述,氮添加会显著改变植物群落地上部分C:N:P化学计量特征,进而影响草地生态系统的功能和过程,但对地下C:N:P化学计量特征几乎没有影响。 | Ziqi Wang Jie Wang Honghui Wu Tian Yang Yixin An Yunlong Zhang Jianlin Bian Ying Li Haiyan Ren Ariuntsetseg Lkhagva Xu Wang Qiang Yu | 2024 | Journal of Plant Ecology2024，17，1： | 0 |
| 10 | The Nonlinear Coupling of Oscillating Bubble and Floating Body with Circular Hole显示文摘The fluid-structure interaction of the oscillating bubble and floating body with circular hole is essentially the nonlinear coupling problem among the incomplete movable boundary,free surface and bubble.This problem is particularly complicated in bubble dynamics.Combined with the volume of fluid method,the Eulerian finite element method is employed to deal with the fluid movement.Based on the improved penalty immersed boundary method,the transient axisymmetric numerical model is established in this paper,considering the fluid-structure interaction effect.The results of simulation are consistent with those of the electric discharge bubble experiment and explosion experiment.Subsequently,considering the influence of the hole size,floating body density,explosive location,and buoyancy,this complex fluid-structure interaction problem is analyzed systematically.Through numerical simulation,we get some new conclusions.When the radius of the hole Rh less than the maximum radius of the oscillating bubble,the changes in the whole system are incredibly intense,and the free surface crushing will emerge.The energy of the bubble acts more on the radial direction of the floating body,when the explosive location parameter is small.When the floating body has the same density as the water,the multiple spike skirt is displayed vividly.And the buoyancy of fluid can produce a lifting effect on the floating body. | Ming He Yunlong Liu Shaofei Ren Wentao Liu | 2021 | Computer Modeling in Engineering & Sciences2021，，6： | 0 |
| 11 | Tetrapyrrole biosynthesis pathway regulates plastid-to-nucleus signaling by controlling plastid gene expression in plants显示文摘Plastid-to-nucleus retrograde signaling coordinates nuclear gene expression with chloroplast developmental status and is essential for the photoautotrophic lifestyle of plants.Previous studies have established that tetrapyrrole biosynthesis(TPB)and plastid gene expression(PGE)play essential roles in plastid retrograde signaling during early chloroplast biogenesis;however,their functional relationship remains unknown.In this study,we generated a series of rice TPB-related gun(genome uncoupled)mutants and systematically analyzed their effects on nuclear and plastid gene expression under normal conditions or when subjected to treatments with norflurazon(NF;a noncompetitive inhibitor of carotenoid biosynthesis)and/or lincomycin(Lin;a specific inhibitor of plastid translation).We show that under NF treatment,expression of plastid-encoded polymerase(PEP)-transcribed genes is significantly reduced in the wild type but is derepressed in the TPB-related gun mutants.We further demonstrate that the derepressed expression of PEPtranscribed genes may be caused by increased expression of the PEP core subunit and nuclear-encoded sigma factors and by elevated copy numbers of plastid genome per haploid genome.In addition,we show that expression of photosynthesis-associated nuclear genes(PhANGs)and PEP-transcribed genes is correlated in the rice TPB-related gun mutants,with or without NF or Lin treatment.A similar correlation between PhANGs and PGE is also observed in the Arabidopsis gun4 and gun5 mutants.Moreover,we show that increased expression of PEP-transcribed plastid genes is necessary for the gun phenotype in NF-treated TPB-related gun mutants.Further,we provide evidence that these TPB-related GUN genes act upstream of GUN1 in the regulation of retrograde signaling.Taken together,our results suggest that the TPB-related GUN genes control retrograde plastid signaling by regulating the PGE-dependent retrograde signaling pathway. | Yunlong Wang Yihua Wang Xiaopin Zhu Yulong Ren Hui Dong Erchao Duan Xuan Teng Huanhuan Zhao Rongbo Chen Xiaoli Chen Jie Lei Hang Yang Yunlu Tian Liangming Chen Xi Liu Shijia Liu Ling Jiang Haiyang Wang Jianmin Wan | 2023 | Plant Communications2023，4，1： | 0 |
| 12 | Expression and Purification of Zinc Finger Domain and Central Domain of MDMD2显示文摘[Objective] This study aimed to construct the His-tagged prokaryotic expression vectors harboring zinc finger domain(ZF) and central domain(acidic domain & zinc finger domain,CT) of MDM2,respectively,and preliminarily identified biologic activity of the purified fusion proteins.[Method]ZF and CT coding regions were amplified from MDM2 c DNA library by PCR and separately inserted into prokaryotic expression vector p ET28 b to construct the recombinant plasmids.After verification by enzyme digestion,the recombinant plasmids were separately transformed into E.coli DH5α competent cells.The expressed recombinant plasmids were purified using Ni-NTA magnetic beads and identified by SDS-PAGE and Western blotting.[Result]The molecular weight of His-ZF and His-CT fusion proteins was 21 and 31 k D,respectively.[Conclusion] Recombinant fusion proteins containing ZF and CT of MDM2 were obtained successfully,which laid the foundation for subsequent protein crystallization and three-dimensional structure analysis. | Xiaoling REN Ting CHEN Yunlong ZHANG Changrui LU Minmin ZHANG Jun ZHENG Xiaoqi QI | 2016 | Agricultural Biotechnology2016，5，2： | 0 |
| 13 | Single-cell technologies:From research to application显示文摘In recent years,more and more single-cell technologies have been developed.A vast amount of single-cell omics data has been generated by large projects,such as the Human Cell Atlas,the Mouse Cell Atlas,the Mouse RNA Atlas,the Mouse ATAC Atlas,and the Plant Cell Atlas.Based on these single-cell big data,thousands of bioinformatics algorithms for quality control,clustering,cell-type annotation,developmental inference,cell-cell transition,cell-cell interaction,and spatial analysis are developed.With powerful experimental single-cell technology and state-of-the-art big data analysis methods based on artificial intelligence,the molecular landscape at the single-cell level can be revealed. | Lu Wen Guoqiang Li Tao Huang Wei Geng Hao Pei Jialiang Yang Miao Zhu Pengfei Zhang Rui Hou Geng Tian Wentao Su Jian Chen Dake Zhang Pingan Zhu Wei Zhang Xiuxin Zhang Ning Zhang Yunlong Zhao Xin Cao Guangdun Peng Xianwen Ren Nan Jiang Caihuan Tian Zi-Jiang Chen | 2022 | The Innovation2022，3，6： | 0 |
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