|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | B-box proteins:Pivotal players in light-mediated development in plants^FA显示文摘Light signals mediate a number of physio-logical and developmental processes in plants, such as flowering, photomorphogenesis, and pigment accu-mulation. Emerging evidence has revealed that a group of B-box proteins (BBXs) function as central players in these light-mediated developmental processes. B-box proteins are a class of zinc-coordinated transcription factors or regulators that not only directly mediate the transcription of target genes but also interact with various other factors to create a complex regulatory network involved in the precise control of plant growth and development. This review summarizes and highlights the recent findings concerning the critical regulatory functions of BBXs in photoperiodic flow-ering, light signal transduction and light-induced pigment accumulation and their molecular modes of action at the transcriptional and post-translational levels in plants. | Zhaoqing Song Yeting Bian Jiujie Liu Yuting Sun Dongqing Xu | 2020 | Journal of Integrative Plant Biology2020，62，9： | 5 |
| 2 | Defect engineering on carbon black for accelerated Li-S chemistry显示文摘Rationally designing sulfur hosts with the functions of confining lithium polysulfides(LiPSs)and promoting sulfur reaction kinetics is critically important to the real implementation of lithium-sulfur(Li-S)batteries.Herein,the defect-rich carbon black(CB)as sulfur host was successfully constructed through a rationally regulated defect engineering.Thus-obtained defect-rich CB can act as an active electrocatalyst to enable the sulfur redox reaction kinetics,which could be regarded as effective inhibitor to alleviate the LiPS shuttle.As expected,the cathode consisting of sulfur and defect-rich CB presents a high rate capacity of 783.8 mA·h·g^−1 at 4 C and a low capacity decay of only 0.07% per cycle at 2 C over 500 cycles,showing favorable electrochemical performances.The strategy in this investigation paves a promising way to the design of active electrocatalysts for realizing commercially viable Li-S batteries. | Wenlong Cai Yingze Song Yuting Fang Weiwei Wang Songlin Yu Huaisheng Ao Yongchun Zhu Yitai Qian | 2020 | Nano Research2020，13，12： | 5 |
| 3 | High-temperature phase transition behavior and magnetocaloric effect in a sub-rapidly solidified La–Fe–Si plate produced by centrifugal casting显示文摘A sub-rapidly solidified La Fe_(11.6)Si_(1.4)plate was fabricated directly from liquid by centrifugal casting method.The phase constitution,microstructure and magnetocaloric effect were investigated using backscatter scanning electron microscopy,X-ray diffraction,differential scanning calorimetry and physical property measurement system.When the plate was annealed at 1373 K,1 phase was formed by a solid-state peritectoid reaction.A first-order magnetic phase transition occurred in the vicinity of188 K,and the effective refrigeration capacities reached 203.5 J/kg and 209.7 J/kg in plates annealed for1 h and 3 h,respectively,under a magnetic field change of 3 T.It is suggested that centrifugal casting may become a new approach to prepare high-performance La–Fe–Si magnetocaloric plates for practical applications,which could largely accelerate the formation of 1 phase during high-temperature heat-treatment process due to refined and homogeneous honeycombed microstructure. | Zhishuai Xu Yuting Dai Yue Fang Zhiping Luo Ke Han Changjiang Song Qijie Zhai Hongxing Zheng | 2018 | Journal of Materials Science & Technology2018，34，8： | 4 |
| 4 | Cold Stress-induced Glucosyltransferase CsUGT78A15 is Involved in the Formation of Eugenol Glucoside in Camellia sinensis显示文摘Eugenol is a natural phenolic compound known for its health-promoting properties and its ability to add a floral scent to tea plants.Plant eugenol glycosides have been identified and shown to make important contributions to fruit floral quality.However,the details of their biosynthesis and metabolism in tea plants are still unknown.Here,eugenol glucoside was unambiguously identified as a native metabolite in the tea plant,and its biosynthesis was shown to be induced by low temperature treatment.Through the analysis of UGTs induced by low temperature,the glycosyltransferase CsUGT78A15 was identified in tea,and its encoded protein was shown to catalyze the glucosylation of eugenol.Vmax/Km ratios showed that eugenol was the most suitable substrate for CsUGT78A15.Sugar donor preference analysis showed that CsUGT78A15 had a higher selectivity for glucose,followed by galactose and glucuronic acid.The expression of CsUGT78A15was correlatedwith the accumulation of eugenol glucoside in different tissues and genotypes of tea.Down-regulation of CsUGT78A15 led to a decreased eugenol glucoside content under cold stress,indicating that CsUGT78A15 plays an important role in the biosynthesis of eugenol glucoside under cold stress.The identification of eugenol glucoside in the tea plant and the discovery of a cold stress-induced eugenol glucosyltransferase in tea provide the foundation for the improvement of tea flavor under cold stress and the biotechnological production of eugenol glucoside. | Mingyue Zhao Binbin Cai Jieyang Jin Na Zhang Tingting Jing Jingming Wang Yuting Pan Zixiang Zhou Yifan Zhao Yingying Feng Feng Yu Mengting Zhang Yating Li Zhonghua Liu Chuankui Song | 2020 | Horticultural Plant Journal2020，6，6： | 4 |
| 5 | Enhanced digestion inhibition and mucus penetration of F127-modified self-nanoemulsions for improved oral delivery显示文摘Self-nanoemulsifying systems(SNEs) have excellent ability to improve the solubility ofpoorly water-soluble drugs(PWSD). However, SNEs are likely to be degraded in gastroin-testinal(GIT) when their surface is recognized by lipase/co-lipase enzyme complex, result-ing in rapid release and precipitation of encapsulated drugs. The precipitates are then cap-tured and removed by intestinal mucus, reducing the delivery efficacy of SNEs. Herein, theamphiphilic polymer Pluronic? F127 was incorporated into long and short-chain triglyc-erides(LCT, SCT) based SNEs to diminish the recognition and therefore minimized theirdegradation by enzymes and clearance by mucus. The SNEs were characterized in termsof particle size, zeta potential and stability. Ex vivo multiple particles tracking studies wereperformed by adding particle solution into fresh rat mucus. Cellular uptake of SNEs wereconducted by using E12 cells, the absorption and distribution in small intestine were alsostudied after oral administration in male Sprague-Dawley(SD) rats. The in vitro digestionrate of SNEs were found to be in following order SCT-SNE > SCT-F127-SNE > LCT-SNE > LCT-F127-SNE. Moreover, the LCT-F127-SNE was found to be most effective in enhancing cellularuptake, resulting in 3.5-fold, 2.1-fold and 1.7-fold higher than that of SCT-SNE, LCT-SNE andSCT-F127-SNE, respectively. After incubating the SNE with E12 cells, the LCT-F127-SNE ex-hibited the highest amount regarding both mucus penetration and cellular uptake, with anuptake amount number(via bicinchoninic acid(BCA) analysis) of 3.5-fold, 2.1-fold and 1.7-fold higher than that of SCT-SNE, LCT-SNE and SCT-F127-SNE, respectively. The in vivo results revealed that orally administered LCT-F127-SNE could significantly increase the bioavailability of Cyclosporine A(CsA), which was approximately 2.43-fold, 1.33-fold and 1.80-fold higher than that of SCT-SNE, SCT-F127-SNE and LCT-SNE, respectively. We address in this work that F127-modified SNEs have potentials to improve oral drug absorption by significantly reducing gastrointestinal enzymatic degradation and simultaneously enhancing mucus penetration. | Wenyi Song Yuting Yang Miaorong Yu Quanlei Zhu Mohammadali Soleimani Damaneh Haijun Zhong Yong Gan | 2018 | Asian Journal of Pharmaceutical Sciences2018，13，4： | 3 |
| 6 | The integration of transcriptomic and transgenic analyses reveals the involvement of the SA response pathway in the defense of chrysanthemum against the necrotrophic fungus Alternaria sp.显示文摘Chrysanthemum morifolium cv.‘Huaihuang’has ornamental,edible,medicinal,and tea product uses.However,its field growth,yield,and quality are negatively affected by black spot disease caused by Alternaria sp.(Strain:HQJH10092301;GenBank accession number:KF688111).In this study,we transcriptionally and transgenically characterized a new cultivar,‘Huaiju 2#’(Henan Traditional Chinese Medicine Plant Cultivar identification number:2016002),which was bred from‘Huaihuang’and shows resistance to Alternaria sp.Numerous‘Huaiju 2#’plants were inoculated with Alternaria sp.for three or five days.Metabolic analysis showed increases in both salicylic acid(SA)and jasmonic acid(JA)in infected plants compared to the control.Protein activity analysis also revealed a significant increase in defense enzyme activities in infected plants.RNA-Seq of plants infected for 3 or 5 days produced a total of 58.6 GB of clean reads.Among these reads,16,550 and 13,559 differentially expressed genes(DEGs)were identified in Cm_3 dpi(sample from 3 days post-inoculation labeled as Cm_3 dpi)and Cm_5 dpi(sample from 5 days post-inoculation labeled as Cm_5 dpi),respectively,compared with their controls(Cm_0 d:a mixture samples from 0 d(before inoculation)and those treated with sterile distilled water at 3 dpi and 5 dpi).Gene annotation and cluster analysis of the DEGs revealed a variety of defense responses to Alternaria sp.infection,which were characterized by increases in resistance(R)proteins and the reactive oxygen species(ROS),Ca2+,mitogen-activated protein kinase(MAPK),and JA signaling pathways.In particular,SA signaling was highly responsive to Alternaria sp.infection.The qPCR analysis of 12 DEG candidates supported their differential expression characterized by using the RNA-Seq data.One candidate was CmNPR1(nonexpressor of pathogenesis-related gene 1),an important positive regulator of SA in systemic acquired resistance(SAR).Overexpression of CmNPR1 in‘Huaiju 2#’increased the resistance of transgenic plants to black spot.These findings indicate that the SA response pathway is likely involved in the defense of‘Huaiju 2#’against Alternaria sp.pathogens. | Xiting Zhao Lingyu Song Liwei Jiang Yuting Zhu Qinghui Gao Dandan Wang Jing Xie Meng Lv Ping Liu Mingjun Li | 2020 | Horticulture Research2020，7，1： | 3 |
| 7 | Staff characteristics and care in Chinese nursing homes: A systematic literature review显示文摘The need for institutional long-term care(LTC)services,including nursing homes(NHs),is increasing in China's Mainland in part due to the aging population and changing family structures.In developing NH staff training programs,a review is needed to synthesize knowledge about staff and resident characteristics,and care provided in NHs.This systematic review aims to describe,in Chinese NHs,(1)NH staff characteristics and the care they provide,(2)resident characteristics and care needs,and (3)the role of family members.The 45 articles included in this review covered both urban and rural areas of China's Mainland.We found that staff in Chinese NHs were older and had lower education levels in comparison with those in western countries.Most direct caregivers in urban areas were migratory workers from rural area.Chinese NHs had few qualification standards for staff preparation for their roles in NHs.Also,functional levels of residents in Chinese NHs were higher compared to NH residents in western countries.In addition,family members played important roles in caring for NH residents.These findings suggest a need for staff development programs that tailor for lower educated NH staff and care needs of residents with widely varying levels in function from independent to bedbound in China's Mainland.The findings also suggest that staff qualification standards require critically policy development to improve the capacity of Chinese NHs to provide competent and safe care. | Yuting Song Ruth AAnderson Kirsten NCorazzini Bei Wu | 2014 | International Journal of Nursing Sciences2014，1，4： | 2 |
| 8 | Observation of coupling between zero- and two- dimensional semiconductor systems based on anomalous diamagnetic effects显示文摘我们报导在一个单个自我装配的 InAs 量点和弄湿的层之间联合的直接观察,基于用磁电机光致发光光谱学的许多身体激子状态的强壮的抗磁的移动。当在弄湿的层的一个电子在量点与一个洞结合时,一个极其大的积极抗磁的系数被观察;系数比在量点限制的激子状态的大将近一个数量级。有在联合系统的一个量点的洞的电子的再结合导致不平常的否定抗磁的效果,它在一个纯量点系统是比那强壮五倍的。这效果能被归因于在再结合以后处于量点的激动的状态在弄湿的层或电子的传播仍然是电子到弄湿的层的 wavefunction 的扩大。在这种情况中,因为洞的缺席,在量点飞机的最后的状态的 wavefunction 程度比起始的状态的大得多吸引电子。在弄湿的层取决于大电子 wavefunction 程度的射出的光子的性质显示联合发生在不同维数的系统之间,它也从在不同配置使用一个磁场获得的结果被验证。这研究铺平一条新道路与零维、二维的结构观察混合状态,它能为调查 Kondo 物理并且实现是有用的基于纺纱固态量信息处理。 | Shuo Cao Jing Tang Yue Sun Kai Peng Yunan Gao Yanhui Zhao Chenjiang Qian Sibai Sun Hassan Ali Yuting Shao Shiyao Wu Feilong Song David A. Williams Weidong Sheng Kuijuan Jin Xiulai Xu | 2016 | Nano Research2016，9，2： | 2 |
| 9 | Microbial community and functional genes in the rhizosphere of alfalfa in crude oil-contaminated soil显示文摘与粗略的污染油的土壤构造的一个 rhizobox 系统与苜蓿(Medicago sativa L.) 被生长在土壤上评估根围效果,并且探索工厂在土壤由提高原油的移动的潜在的机制微生物引起的人口和功能的结构。在 80 天的实验期间, 31.6% 油从邻近的根围(AR ) 被移开;这价值是 27% , 53%higher 分别地从远根围(FR ) 并且从非根围(NR ) 比油的百分比搬迁了。不是自养的细菌和降级烃的细菌的人口比在 NR 在 AR 和 FR 是更高的。然而,原油的移动率断然在根围与降级烃的细菌的比例被相关。总共, 796, 731,和 379 功能的基因被 microarray 分别地在 AR, FR,和 NR 检测。与碳降级和器官的补习有关的功能的基因的更高的比例,与 NR 土壤相比在根围土壤被发现,建议根围有选择地增加了许多这些特定的功能的基因。保持水的能力的增加和在土壤的 pH 以及咸度的减少都跟随了 AR 的顺序 > FR > NR。正规部件分析证明那咸度是在根围影响微生物引起的功能的结构的最重要的环境因素,那咸度否定地与许多碳和器官的降级基因被相关。 | YiZHONG Jian WANG Yizhi SONG Yuting LIANG Guanghe LI | 2012 | Frontiers of Environmental Science & Engineering2012，6，6： | 2 |
| 10 | Investigation of hemostatic effect of Spleeninvigorating,Qi-replenishing and Bloodarresting Formula on simvastatin-induced zebrafish hemorrhage model显示文摘Blood-arresting Formula on simvastatin-induced zebrafish hemorrhage model compared with that of the Heat-clearing and Blood-cooling Formula.Methods:AB strain wild type zebrafish were treated with 0.5 mM simvastatin for 24 hours to establish the zebrafish hemorrhage model.Under strictly blind experimental conditions,the zebrafish were then treated with experimental agents in different concentration within the maximum non-lethal dose.The intervention effect of the Spleen-invigorating,Qi-replenishing and Blood-arresting Formula was comprehensively assessed by examining the main observational parameters such as the bleeding reduction rate and hemostatic rate while referring to the additional parameters such as blood flow,improvement rate of blood flow,velocity of movement,improvement rate of motion under spleen-qi deficiency.Results:Hemostatic effect:The bleeding rate and hemostatic rate in the zebrafish were respectively,30%,15%,45%,40%and 60%,80%,40%,47%when equal concentration of 500 mg/mL,1000 mg/mL were used.This showed that the experimental herbs B1 was superior to B2 in decreasing the bleeding rate and improving hemostatic effect in the zebrafish.Improvement of blood flow:With equal concentration,the experimental herb B1 was superior to B2 in increasing the blood flow and improvement rate of blood flow in hemorrhagic zebrafish.Promotion of the improvement of motion:With equal concentration of 500 mg/mL and 1000 mg/mL,the experimental herb B1 was superior to B2 in promoting the velocity of motion in the zebrafish and promoting the improvement rate of motion.Conclusion:The manifestations of simvastatin-induced zebrafish hemorrhage are basically similar to that of failure of the spleen to control blood in human beings.The Spleeninvigorating,Qi-replenishing and Blood-arresting Formula has good hemostatic effect on simvastatin-induced hemorrhage in the zebrafish.It also boasts the functions of improving blood flow and velocity of motion in the hemorrhagic zebrafish.Such a conclusion provides the experimental basis for the treatment of the syndrome of failure of the spleen to control blood by this formula. | Yuting Chu Ling Zhang Xiaoyu Zhu Yayue Zhang Bo Xia Li Hou Rushun Song Tiantian Li Chunqi Li Qing Dong a Xinyi Chen | 2016 | Journal of Traditional Chinese Medical Sciences2016，3，4： | 1 |
| 11 | Affinity adsorption of bromelain on Reactive Red 120 immobilized magnetic composite particles显示文摘 | SONG Mingmin NIE Huali ZHOU Yuting | 2011 | Separation Science and Technology2011，46，： | 1 |
| 12 | Responses of the methanogenic pathway and fraction of CH4 oxidization in a flooded paddy soil to rice planting显示文摘Rice planting(RP)is significant to methane(CH4_(4))emissions from paddy fields,but its effect on the relative contribution of the acetoclastic methanogenesis to total CH4_(4) production(F_(ac))and the fraction of CH4_(4) oxidized(Fox)is poorly understood.To quantify the responses of the F_(ac) and Fox to RP,we investigated CH4_(4) fluxes,CH4_(4) production and oxidation potentials,dissolved CH4_(4) concentrations,and their stable carbon isotopes in a flooded paddy soil.The mcrA and pmoA gene copies were also determined by quantitative polymerase chain reaction(qPCR).Compared with the unplanted soil(control,CK),the seasonal CH4_(4) emissions from the planted soil were significantly enhanced,13.6 times,resulting in large decreases in the CH4_(4) concentrations in the soil solution.This indicated that much more CH4_(4) was released into the atmosphere by the RP than was stored in the soils.Acetoclastic methanogenesis became more important from the tillering stage(TS)to the ripening stage(RS)for the CK,with F_(ac) values increased from 17%-20% to 46%-55%.With RP,the F_(ac) values were enhanced by 10%-20%,and it significantly increased the copy numbers of the mcrA gene at the four rice stages(TS,booting stage(BS),grain-filling stage(GS),and RS).Furthermore,the effect of the RP on the abundance of the mcrA gene was highly concurrent with the effect on the F_(ac) values.At the TS,the Fox values at the soil-water interface were around 50%-75% for the CK,being 15%-20% lower than those of the RP in the rhizosphere.It increased to 65%-100% at the GS,but was reduced by 20%-30% after the RP.These differences might be because the copy numbers of the pmoA gene were significantly raised at the TS while lowered at the GS by the RP.This was further demonstrated by the strong correlations between the effect of the RP on the abundance of the pmoA gene and the effect on the Fox values.These findings suggest that RP markedly impacts on the abundances of the mcrA and pmoA genes,affecting the pathway of CH4_(4) production and the fraction of CH4_(4) oxidization,respectively. | Yuting YANG Guangbin ZHANG Jing MA Qiong HUANG Haiyang YU Kaifu SONG Xiaoli ZHU Xi MIAO Hua XU | 2021 | Pedosphere2021，31，6： | 1 |
| 13 | Selective oxidation of pyridine to pyridine-N-oxide with hydrogen peroxide over Ti-MWW catalyst显示文摘 | Xie Wei Zheng Yuting Zhao Song | 2010 | Catalysis Today2010，157，： | 1 |
| 14 | Anthocyanin biosynthesis in pears is regulated by a R2R3-MYB transcription factor PyMYB10显示文摘 | Shouqian Feng Yanling Wang Song Yang Yuting Xu Xuesen Chen | 2010 | Planta2010，，1： | 1 |
| 15 | Mutant SOD1 G93A triggers mitochondrial fragmentation in spinal cord motor neurons: Neuroprotection by SIRT3 and PGC-1α显示文摘 | Wenjun Song Yuting Song Brad Kincaid Blaise Bossy Ella Bossy-Wetzel | 2012 | Neurobiology of Disease2012，，： | 1 |
| 16 | Promoter and coding sequence diversity of CsCCD1 may contribute to the differential accumulation of floralβ-ionone in fresh tea leaves显示文摘The carotenoid-derived volatileβ-ionone makes an important contribution to tea fragrance.Here,we qualitatively and quantitatively analysed 15 carotenoids in tea leaves of 13 cultivars by UHPLC-APCI-MS/MS.The 13 cultivars were divided into two groups by PCA(Principal Component Analysis)clustering analysis of their carotenoid content,and OPLS-DA(Orthogonal projections to latent structures)indicated that the levels ofβ-carotene(VIP=2.89)and lutein(VIP=2.30)were responsible for much of the variation between the two groups.Interestingly,theβ-carotene toβ-ionone conversion rates in Group 1 were higher than in Group 2,while theβ-carotene content was significantly lower in Group 1 than in Group 2.Theβ-ionone content was significantly higher in Group 1.Pearson Correlation Coefficient calculation between the transcription level of candidate genes(CsCCD1 and CsCCD4)and the accumulation ofβ-ionone indicated that CsCCD1 may involve in the formation ofβ-ionone in 13 cultivars.Prokaryotic expression and in vitro enzyme activity assays showed that‘Chuanhuang 1’had an amino acid mutation in carotenoid cleavage dioxygenases 1(CsCCD1)compared with‘Shuchazao’,resulting in a significantly higherβ-ionone content in‘Chuanhuang 1’.Sequence analysis showed that‘Chuanhuang 1’and‘Huangdan’had different CsCCD1 promoter sequences,leading to significantly higher CsCCD1 expression andβ-ionone accumulation in‘Chuanhuang 1’.These results indicated that the promoter and coding sequence diversity of CsCCD1 might contribute to the differential accumulation ofβ-ionone in different tea cultivars. | Jingming Wang Mingyue Zhao Ting Gao Yingying Feng Feiquan Wang Yuting Pan Jieyang Jin Tingting Jing Mengqian Lu Mengting Zhang Danyang Guo Xiaochun Wan Wilfried Schwab Chuankui Song | 2023 | Horticultural Plant Journal2023，9，4： | 1 |
| 17 | Understanding the geometric and electronic factors of PtNi bimetallic surfaces for efficient and selective catalytic hydrogenation of biomass-derived oxygenates显示文摘Ni-base catalysts are promising candidate for the hydrogenation of furfural(FAL) to high-value chemicals.However,slow intermediate desorption and low selectivity limit its implementation.Identifying the catalytic performance of each active sites is vital to design hydrogenation catalyst,and tuning the geometrical sites at molecule level in PtNi could lead to the modification of electronic structure,and thus the selectity for the hydrogenation of FAL was modulated.Herein,PtNi hollow nanoframes(PtNi HNFs) with three dimensional(3 D) molecular accessibility were synthesized,EDX results suggested that Ni was evenly distributed inside of the hollow nanoframes,whereas Pt was relatively concentrated at the edges.DFT calculation demonstrated that PtNi significant decrease the desorption energy of the intermediates.This strategy could not only enhance the desorption of intermediates to improve the catalytic performance,but also transfer the adsorption mode of FAL on catalyst surface to selective hydrogenation of FAL to FOL or THFA.The PtNi HNFs catalyst afforded excellent catalytic performance for selective hydrogenation of a broad range of biomass-derived platform chemicals under mild conditions,especially of FAL to furfuryl alcohol(FOL),in quantitative FOL yields(99%) with a high TOF of 2.56 h^(-1).It is found that the superior performance of PtNi HNFs is attributed to its 3 D hierarchical structure and synergistic electronic effects between Pt and Ni.Besides,the kinetic study demonstrated that the activation energy for hydrogenation of FAL was as low as 54.95 kJ mol^(-1). | Jingcheng Wu Chuangwei Liu Yuting Zhu Xiangbo Song Chengyan Wen Xinghua Zhang Chenguang Wang Longlong Ma | 2021 | Journal of Energy Chemistry2021，30，9： | 1 |
| 18 | Unveiling E2F4,TEAD1 and AP-1 as regulatory transcription factors of the replicative senescence program by multi-omics analysis显示文摘Senescence,a stable state of growth arrest,affects many physiological and pathophysiological processes,especially aging.Previous work has indicated that transcription factors(TFs)play a role in regulating senescence.However,a systematic study of regulatory TFs during replicative senescence(RS)using multiomics analysis is still lacking.Here,we generated timeresolved RNA-seq,reduced representation bisulfite sequencing(RRBS)and ATAC-seq datasets during RS of mouse skin fibroblasts,which demonstrated that an enhanced inflammatory response and reduced proliferative capacity were the main characteristics of RS in both the transcriptome and epigenome.Through integrative analysis and genetic manipulations,we found that transcription factors E2F4,TEAD1 and AP-1 are key regulators of RS.Overexpression of E2f4 improved cellular proliferative capacity,attenuated SA-β-Gal activity and changed RS-associated differentially methylated sites(DMSs).Moreover,knockdown of Tead1 attenuated SA-β-Gal activity and partially altered the RS-associated transcriptome.In addition,knockdown of Atf3,one member of AP-1 superfamily TFs,reduced Cdkn2a(p16)expression in pre-senescent fibroblasts.Taken together,the results of this study identified transcription factors regulating the senescence program through multi-omics analysis,providing potential therapeutic targets for anti-aging. | Yuting Wang Liping Liu Yifan Song Xiaojie Yu Hongkui Deng | 2022 | Protein & Cell2022，13，10： | 1 |
| 19 | Hierarchical Ti_(3)C_(2)T_(x)@MoS_(2) heterostructures:A first principles calculation and application in corrosion/wear protection显示文摘Surface and interface engineering plays a crucial role in modulating the properties of materials,especially two-dimensional(2D)materials.Hence,a strategy,forming heterostructures with MoS_(2),is proposed to overcome the natural agglomeration of Ti_(3)C_(2)T_(x) MXene nanosheets.Most importantly,the interactions between Ti_(3)C_(2)Tx and MoS_(2) were elaborately investigated by first-principles calculations based on density functional theory(DFT)for the first time.The calculations demonstrate that van der Waals forces dominate the interface interactions of Ti_(3)C_(2)T_(x) and MoS_(2),rendering Ti_(3)C_(2)T_(x)@MoS_(2) heterostructures favorable stability.The Ti_(3)C_(2)T_(x)@MoS_(2) heterostructure composites were synthesized through a facile one-step hydrothermal method and exhibit a 2D hierarchical structure.Furthermore,the corrosion and tribological properties of epoxy composite coatings with varying proportions of Ti_(3)C_(2)T_(x)@MoS_(2) composites were studied in detail.As a result,the epoxy composite coating with 0.1 wt.%Ti_(3)C_(2)T_(x)@MoS_(2) composites(Ti_(3)C_(2)T_(x)@MoS_(2)-0.1)exhibits excellent corrosion protection and antiwear performances.The Ti_(3)C_(2)T_(x)@MoS_(2)-0.1 keeps the largest low-frequency impedance modulus(|Z|_(0.)01 Hz)and coating resistance(R_(c))during the whole immersion period.Its wear rate is 0.09μm^(3)/(Nμm)under the load of 10 N,one half of that of pure epoxy coating(EP).This work further broadens the application of MXene-based heterostructure composites. | Meng Cai Peng Feng Han Yan Yuting Li Shijie Song Wen Li Hao Li Xiaoqiang Fan Minhao Zhu | 2022 | Journal of Materials Science & Technology2022，，21： | 1 |
| 20 | USP7 deubiquitinates and stabilizes NOTCH1 in T-cell acute lymphoblastic leukemia显示文摘T-cell acute lymphoblastic leukemia(T-ALL)is a highly aggressive leukemia that is primarily caused by aberrant activation of the NOTCH1 signaling pathway.Recent studies have revealed that posttranslational modifications,such as ubiquitination,regulate NOTCH1 stability,activity,and localization.However,the specific deubiquitinase that affects NOTCH1 protein stability remains unestablished.Here,we report that ubiquitin-specific protease 7(USP7)can stabilize NOTCH1.USP7 deubiquitinated NOTCH1 in vivo and in vitro,whereas knockdown of USP7 increased the ubiquitination of NOTCH1.USP7 interacted with NOTCH1 protein in T-ALL cells,and the MATH and UBL domains of USP7 were responsible for this interaction.Depletion of USP7 significantly suppressed the proliferation of T-ALL cells in vitro and in vivo,accompanied by downregulation of the NOTCH1 protein level.Similarly,pharmacologic inhibition of USP7 led to apoptosis of T-ALL cells.More importantly,we found that USP7 was significantly upregulated in human T-ALL cell lines and patient samples,and a USP7 inhibitor exhibited cell cytotoxicity toward primary T-ALL cells,indicating the clinical relevance of these findings.Overall,our results demonstrate that USP7 is a novel deubiquitinase that stabilizes NOTCH1.Therefore,USP7 may be a promising therapeutic target in the currently incurable T-ALL. | Huizhuang Shan Xiangyun Li Xinhua Xiao Yuting Dai Jinyan Huang Junjun Song Meng Liu Li Yang Hu Lei Yin Tong Li Zhou Hanzhang Xu Yingli Wu | 2018 | Signal Transduction and Targeted Therapy2018，3，1： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员