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| 1 | Akt Inhibitor Perifosine Prevents Epileptogenesis in a Rat Model of Temporal Lobe Epilepsy显示文摘Accumulating data have revealed that abnormal activity of the mTOR(mammalian target of rapamycin)pathway plays an important role in epileptogenesis triggered by various factors. We previously reported that pretreatment with perifosine, an inhibitor of Akt(also called protein kinase B), abolishes the rapamycin-induced paradoxical increase of S6 phosphorylation in a rat model induced by kainic acid(KA). Since Akt is an upstream target in the mTOR signaling pathway, we set out to determine whether perifosine has a preventive effect on epileptogenesis. Here, we explored the effect of perifosine on the model of temporal epilepsy induced by KA in rats and found that pretreatment with perifosine had no effect on the severity or duration of the KA-induced status epilepticus. However, perifosine almost completely inhibited the activation of p-Akt and p-S6 both acutely and chronically following the KA-induced status epilepticus.Perifosine pretreatment suppressed the KA-induced neuronal death and mossy fiber sprouting. The frequency of spontaneous seizures was markedly decreased in rats pretreated with perifosine. Accordingly, rats pretreated with perifosine showed mild impairment in cognitive functions. Collectively, this study provides novel evidence in a KA seizure model that perifosine may be a potential drug for use in anti-epileptogenic therapy. | Feng Zhu Jiejing Kai Linglin Chen Meiling Wu Jingyin Dong Qingmei Wang Ling-Hui Zeng | 2018 | Neuroscience Bulletin2018，34，2： | 8 |
| 2 | MRI结合数字化钼靶诊断乳腺癌的临床研究(英文)显示文摘目的将在乳癌的察觉和诊断比较数字早期胸部肿瘤Ⅹ射线测定法和 MRI 的有效性并且象二的联合一样估计这些形式的价值。有外科和病理的 57 个病人证明了胸损害的方法(恶意, n = 32;良性, n = 46 ) 经历了数字早期胸部肿瘤Ⅹ射线测定法和 MRI,脉搏序列包括了 T 1 WI, T 2 WI,弥漫的加权的成像(DWI ) ,和在外科前的动态提高对比的 MRI。这二种形式的结果,所有损害根据 BI-RADS 分类被分类进 5 个组,并且成像调查结果被相关到组织病理学说。每种形式的敏感以及二的联合是计算的。这 78 乳房损害的结果,敏感为数字早期胸部肿瘤Ⅹ射线测定法是 78.13%%(25/32 ) 并且 93.75%(30/32 ) 为 MRI (P >
0.05 ) 。特性是 73.91%%(34/46 ) 并且 89.13%(41/46 ) 因此(P <
0.05 ) ,他们俩出现了统计差别。当这二种形式在联合被使用,敏感和特性分别地是 98.63% 和 97.16% 。在有 MRI 的联合的结论数字早期胸部肿瘤Ⅹ射线测定法在乳癌,敏感和特性的诊断是有用的当时,被提高与单个形式的相比。 | Fang Ye Mengsu Zeng Fuhua Yan Wei Feng Meiling Zhou Renchen Li Wenfang Chen | 2008 | The Chinese-German Journal of Clinical Oncology2008，7，5： | 3 |
| 3 | 紫杉醇脂质体与紫杉醇联合铂类同步放化疗治疗宫颈癌的随机对照研究(英文)显示文摘Objective:The aim of the study was to compare the efficacy,side effect and influence on the survival rate of two chemotherapy regimens,paclitaxel liposome combined with platinum and paclitaxel combined with platinum,in concurrent chemoradiotherapy for cervical carcinoma.Methods:The 162 cases with primary cervical carcinoma diagnosed between January 2008 and November 2009 in Jiangxi Maternal and Child Health Hospital(China) were enrolled in this randomized controlled trial.Seventy-one cases were included in paclitaxel group and 91 in paclitaxel liposome group.And the chemotherapy doses were as follows:paclitaxel liposome and paclitaxel 135 mg/m2;cisplatin 80 mg/m2 or carboplatin AUC 4-6;then repeated every 21 days for two or three times.Radical radiotherapy was given to both groups at the same time.Efficacy was evaluated according to the tumor regression six months later and follow-up was done consistently.Results:The overall response rates of paclitaxel group and paclitaxel liposome group were 90.1% and 89 % respectively(P > 0.05).The one year cumulative survival was 91.4% for paclitaxel group and 89.2% for paclitaxel liposome group(P > 0.05).The incidence rates of adverse effects such as rash,gastrointestinal toxicity,bone marrow suppression and muscle/joint pain in paclitaxel liposome group were much lower than those in paclitaxel group(P < 0.05),while there was no difference regarding hair loss,hepatic function damage,peripheral neuritis and other aspects(P > 0.05).Conclusion:Paclitaxel liposome plus platinum is a safe and effective method for staging IIa-IV cervical carcinomas.While the long-term efficacy should be further observed. | Siyuan Zeng Ling Li Meiling Zhong Wei Jiang Yun Xiao | 2012 | The Chinese-German Journal of Clinical Oncology2012，11，2： | 3 |
| 4 | Suppression of PFKFB3-driven glycolysis restrains endothelial-to-mesenchymal transition and fibrotic response显示文摘Endothelial-to-mesenchymal transition(EndoMT),the process wherein endothelial cells lose endothelial identity and adopt mesenchymal-like phenotypes,constitutes a critical contributor to cardiac fibrosis.The phenotypic plasticity of endothelial cells can be intricately shaped by alteration of metabolic pathways,but how endothelial cells adjust cellular metabolism to drive EndoMT is incompletely understood.Here,we identified 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3(PFKFB3)as a critical driver of EndoMT via triggering abnormal glycolysis and compromising mitochondrial respiration.Pharmacological suppression of PFKFB3 with salvianolic acid C(SAC),a phenolic compound derived from Salvia miltiorrhiza,attenuates EndoMT and fibrotic response.PFKFB3-haplodeficiency recapitulates the anti-EndoMT effect of SAC while PFKFB3-overexpression augments the magnitude of EndoMT and exacerbates cardiac fibrosis.Mechanistically,PFKFB3-driven glycolysis compromises cytoplasmic nicotinamide adenine dinucleotide phosphate(reduced form,NADPH)production via hijacking glucose flux from pentose phosphate pathway.Efflux of mitochondrial NADPH through isocitrate/α-ketoglutarate shuttle replenishes cytoplasmic NADPH pool but meanwhile impairs mitochondrial respiration by hampering mitochondrial iron-sulfur cluster biosynthesis.SAC disrupts PFKFB3 stability by accelerating its degradation and thus maintains metabolic homeostasis in endothelial cells,underlying its anti-EndoMT effects.These findings for the first time identify the critical role of PFKFB3 in triggering EndoMT by driving abnormal glycolysis in endothelial cells,and also highlight the therapeutic potential for pharmacological intervention of PFKFB3(with SAC or other PFKFB3 inhibitors)to combat EndoMT-associated fibrotic responses via metabolic regulation. | Hao Zeng Ting Pan Meiling Zhan Renaguli Hailiwu Baolin Liu Hua Yang Ping Li | 2022 | Signal Transduction and Targeted Therapy2022，7，10： | 1 |
| 5 | Greater Bay Area Gaining Traction显示文摘ON July 1,2017,Chinese President Xi Jinping attended the signing ceremony of the framework agreement on the development of the Guangdong-Hong Kong-Macao Greater Bay Area.Since then,Guangdong Province has pooled its resources and pulled out all the stops to advance this initiative. | WU ZHE CHEN XIAO ZENG MEILING | 2020 | China Today2020，69，12： | 0 |
| 6 | Targeting cancer cell plasticity by HDAC inhibition to reverse EBV-induced dedifferentiation in nasopharyngeal carcinoma显示文摘Application of differentiation therapy targeting cellular plasticity for the treatment of solid malignancies has been lagging.Nasopharyngeal carci noma(NPC)is a distinctive cancer with poor differe ntiatio n and high prevalenee of Epstein-Barr virus(EBV)infection.Here,we show that the expressi on of EBV latent protein LMP1 in duces dediffere ntiated and stem-like status with high plasticity through the transcriptional inhibition of CEBPA.Mechanistically,LMP1 upregulates STAT5A and recruits HDAC 1/2 to the CEBPA locus to reduce its histone acetylation.HDAC inhibition restored CEBPA expression,reversing cellular dedifferentiation and stem-like status in mouse xeno graft models.These fin dings provide a novel mecha nistic epigenetic-based in sight into virus-induced cellular plasticity and propose a promising concept of differentiation therapy in solid tumor by using HDAC inhibitors to target cellular plasticity. | Jiajun Xie Zifeng Wang Wenjun Fan Youping Liu Fang Liu Xiangbo Wan Meiling Liu Xuan Wang Deshun Zeng Van Wang Bin He Min Yan Zijian Zhang Mengjuan Zhang Zhijie Hou Chunli Wang Zhijie Kang Wenfeng Fang Li Zhang Eric W-F Lam Xiang Guo Jinsong Yan Yixin Zeng Mingyuan Chen Quentin Liu | 2021 | Signal Transduction and Targeted Therapy2021，6，10： | 0 |
| 7 | Decision Difficulty, Work-Family Conflict, and Social Support for Chinese Nurses in Guangzhou after the Two-Child Policy显示文摘Background:AlthoughChina's Mainland implemented a two-child policy (every legally married may have two children) on January 1, 2016, women in China are becoming increasingly cautious in their decision-making about family planning, due to work-family conflicts and a lack of social support. Objective:The aim of this study was to investigate Chinese nurses' intents to have a second child, and to explore the relationship between decision making difficulties in terms of childbearing intents, work-family conflict, and social support for Chinese nurses. Methods:Data were collected at three different hospital levels in four districts of Guangzhou, from 711 nurses of childbearing age (18-50 years) who had had at least one child. A structured questionnaire was used, composed of the Decisional Conflict Scale (DCS), Work-Family Conflict Scale (WFCS), and Scale of Perceived Social Support (SPSS). Results:Participants’ mean age was 33.6 years (18-50). The mean scores of decisional conflict by DCS, work-family conflict by WFCS and perceived social support by SPSS were 40.9 (SD = 14.8), 55.83 (SD = 15.18) and 57.85 (SD = 15.77), respectively. Work-family conflict was positively correlated with decisional conflict (P < 0.01), and social support was negatively related to decisional conflict (P < 0.01). By logistic regression analysis, family interference with work (FIW), especially time-based FIW, was a significant predictor of nurses having decisional conflicts in their childbearing intents (OR=1.083, 95% CI: 1.045 to 1.123). Perceived social support was another significant predictor of decisional difficulty (OR=0.981, 95% CI: 0.970 to 0.992). Discussion:This study found that female nurses, working night shifts and with temporary job contract had higher decisional conflicts in intents of having a second child. The results showed that for Chinese nurses, work-family conflict influences decisional conflicts about having a second child. More importance should be attached to fulfill nurse’s needs of bearing children. | Xiayin Jian Meiling Huang Thomas KS Wong Jing Hu Yingchun Zeng | 2018 | Journal of Nursing2018，7，1： | 0 |
| 8 | Self-sufficient nanoparticles with dual-enzyme activity trigger radical storms and activate cascade-amplified antitumor immunologic responses显示文摘Radiotherapy(RT) can potentially induce systemic immune responses by initiating immunogenic cell death(ICD) of tumor cells.However,RT-induced antitumor immunologic responses are sporadic and insufficient against cancer metastases.Herein,we construct multifunctional self-sufficient nanoparticles(MARS) with dual-enzyme activity(GOx and peroxidase-like) to trigger radical storms and activate the cascade-amplified systemic immune responses to suppress both local tumors and metastatic relapse.In addition to limiting the Warburg effect to actualize starvation therapy,MARS catalyzes glucose to produce hydrogen peroxide(H_(2)O_(2)),which is then used in the Cu^(+)-mediated Fenton-like reaction and RT sensitization.RT and chemodynamic therapy produce reactive oxygen species in the form of radical storms,which have a robust ICD impact on mobilizing the immune system.Thus,when MARS is combined with RT,potent systemic antitumor immunity can be generated by activating antigen-presenting cells,promoting dendritic cells maturation,increasing the infiltration of cytotoxic T lymphocytes,and reprogramming the immuno suppre ssive tumor microenvironment.Furthermore,the synergistic therapy of RT and MARS effectively suppresses local tumor growth,increases mouse longevity,and results in a 90% reduction in lung metastasis and postoperative recurrence.Overall,we provide a viable approach to treating cancer by inducing radical storms and activating cascade-amplified systemic immunity. | Liping Bai Jin Yang Siting Yu Zhongzheng Xiang Yuanyuan Zeng Meiling Shen Xiaorong Kou Qinjie Wu Changyang Gong | 2024 | Acta Pharmaceutica Sinica B2024，14，2： | 0 |
| 9 | Dynamic changes in transposable element and gene methylation in mulberry (Morus notabilis) in response to Botrytis cinerea显示文摘DNA methylation has been proposed to regulate plant stress resistance.However,the dynamic changes in DNA methylation in woody plants and their correlations with pathogenic responses are not fully understood.Here,we present single-base maps of the DNA methylomes of mulberry(Morus notabilis)leaves that were subjected to a mock treatment or inoculation with Botrytis cinerea.Compared with the former,the latter showed decreased mCG and mCHG levels and increased mCHH levels.DNA methylation inhibitors reduced resistance gene methylation levels and enhanced mulberry resistance,suggesting that the hypomethylation of resistance genes affects mulberry resistance to B.cinerea.Virus-induced gene silencing of MnMET1 enhanced the expression of mulberry-resistance genes,thereby increasing the plant’s resistance to B.cinerea.We also found that MITEs play a dominant role in controlling DNA methylation levels.MITEs appear to be the main sources of 24-nt siRNAs that regulate gene expression through the RNA-directed DNA methylation pathway. | Youchao Xin Bi Ma Qiwei Zeng Wenmin He Meiling Qin Ningjia He | 2021 | Horticulture Research2021，8，1： | 0 |
| 10 | An ultra low-input method for global RNA structure probing uncovers Regnase-1-mediated regulation in macrophages显示文摘To enable diverse functions and precise regulation,an RNA sequence often folds into complex yet distinct structures in different cellular states.Probing RNA in its native environment is essential to uncovering RNA structures of biological contexts.However,current methods generally require large amounts of input RNA and are challenging for physiologically relevant use.Here,we report smartSHAPE,a new RNA structure probing method that requires very low amounts of RNA input due to the largely reduced artefact of probing signals and increased efficiency of library construction.Using smartSHAPE,we showcased the profiling of the RNA structure landscape of mouse intestinal macrophages upon inflammation,and provided evidence that RNA conformational changes regulate immune responses.These results demonstrate that smartSHAPE can greatly expand the scope of RNA structure-based investigations in practical biological systems,and also provide a research paradigm for the study of post-transcriptional regulation. | Meiling Piao Pan Li Xiaomin Zeng Xi-Wen Wang Lan Kang Jinsong Zhang Yifan Wei Shaojun Zhangas Lei Tang Jianghui Zhu Chun Kit Kwok Xiaoyu Hu Qiangfeng Cliff Zhang | 2022 | Fundamental Research2022，2，1： | 0 |
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