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| 1 | Chimeric antigen receptor T cell targeting EGFRvIII for metastatic lung cancer therapy显示文摘Lung cancer is the most common incident cancer and the leading cause of cancer death. In recent years, the development of tumor immunotherapy especially chimeric antigen receptor T (CAR-T) cell has shown a promising future. Epidermal growth factor receptor variant III (EGFRvlll) is a tumor-specific mutation expressed in various types of tumors and has been detected in non-small cell lung cancer with a mutation rate of 10%. Thus, EGFRvIII is a potential antigen for targeted lung cancer therapy. In this study, CAR vectors were constructed and transfected into virus-packaging cells. Then, activated T cells were infected with retrovirus harvested from stable virus-producing single clone cell lines. CAR expression on the surfaces of the T cells was detected by flow cytometry and Western blot. The function of CAR-T targeting EGFRvIII was then evaluated. The EGFRvIII-CAR vector was successfully constructed and confirmed by DNA sequencing. A stable virus-producing cell line was produced from a single clone by limited dilution. The culture conditions for the cell line, including cell density, temperature, and culture medium were optimized. After infection with retrovirus, CAR was expressed on more than 90% of the T cells. The proliferation of CAR-T cells were induced by cytokine and specific antigen in vitro. More importantly, EGFRvIII-CART specifically and efficiently recognized and killed A549-EGFRvIII cells with an effector/target ratio of 10:1 by expressing and releasing cytokines, including perforin, granzyme B, IFN-γ, and TNF-α. The in vivo study indicated that the metastasis of A549-EGFRvIII cells in mice were inhibited by EGFRvIII-CART cells, and the survival of the mice was significantly prolonged with no serious side effects. EGFRvIII-CART showed significantly efficient antitumor activity against lung cancer cells expressing EGFRvlll in vivo and in vitro. Therefore, CAR-T targeting EGFRvIII is a potential therapeutic strategy in preventing recurrence and metastasis of lung cancer after surgery. | Zhao Zhang Jun Jiang Xiaodong Wu Mengyao Zhang Dan Luo Renyu Zhang Shiyou Li Youwen He Huijie Bian Zhinan Chen | 2019 | Frontiers of Medicine2019，13，1： | 11 |
| 2 | miR-27 inhibits adipocyte differentiation via suppressing CREB expression显示文摘miR-27 在 adipogenesis 的规定起一个否定作用。然而,分子的机制尚待是 clarified.In 现在的学习,我们发现 miR-27 由镇压部分禁止 adipogenesis 由直接指向它的 3 untranslated 区域的早 adipogenic transcriptionfactor 营地反应元素绑定蛋白质。另外,我们 demonstratedthat 肿瘤坏死因素 --(TNF-) 通过 NF-B 小径的处理起来调整 miR-27。而且, anti-miR-27 reducesthe TNF -- adipogenesis 的导致的抑制。同时, miR-27 表示的层次在成熟 adipocytesof 被减少肥胖的老鼠什么时候与瘦老鼠相比。我们的数据在 adipogenesis 的规定揭示了 miR-27 的新奇机制。 | Yingjie Zhu Xiaodong Zhang Xingpo Ding Huichao Wang Xiantao Chen Hanzheng Zhao Yudong Jia Sanhong Liu Youwen Liu | 2014 | Acta Biochimica et Biophysica Sinica2014，46，7： | 5 |
| 3 | Active terahertz beam steering based on mechanical deformation of liquid crystal elastomer metasurface显示文摘Active metasurfaces are emerging as the core of next-generation optical devices with their tunable optical responses and flat-compact topography.Especially for the terahertz band,active metasurfaces have been developed as fascinating devices for optical chopping and compressive sensing imaging.However,performance regulation by changing the dielectric parameters of the integrated functional materials exhibits severe limitations and parasitic losses.Here,we introduce a C-shape-split-ring-based phase discontinuity metasurface with liquid crystal elastomer as the substrate for infrared modulation of terahertz wavefront.Line-focused infrared light is applied to manipulate the deflection of the liquid crystal elastomer substrate,enabling controllable and broadband wavefront steering with a maximum output angle change of 22°at 0.68THz.Heating as another control method is also investigated and compared with infrared control.We further demonstrate the performance of liquid crystal elastomer metasurface as a beam steerer,frequency modulator,and tunable beam splitter,which are highly desired in terahertz wireless communication and imaging systems.The proposed scheme demonstrates the promising prospects of mechanically deformable metasurfaces,thereby paving the path for the development of reconfigurable metasurfaces. | Xiaolin Zhuang Wei Zhang Kemeng Wang Yangfan Gu Youwen An Xueqian Zhang Jianqiang Gu Dan Luo Jiaguang Han Weili Zhang | 2023 | Light(Science & Applications)2023，12，1： | 4 |
| 4 | 2D CoOOH Sheet-Encapsulated Ni2P into Tubular Arrays Realizing 1000 mA cm^-2-Level-Current-Density Hydrogen Evolution Over 100 h in Neutral Water显示文摘Water electrolysis at high current density(1000 mA cm-2 level)with excellent durability especially in neutral electrolyte is the pivotal issue for green hydrogen from experiment to industrialization.In addition to the high intrinsic activity determined by the electronic structure,electrocatalysts are also required to be capable of fast mass transfer(electrolyte recharge and bubble overflow)and high mechanical stability.Herein,the 2D CoOOH sheet-encapsulated Ni2P into tubular arrays electrocatalytic system was proposed and realized 1000 mA cm-2-levelcurrent-density hydrogen evolution over 100 h in neutral water.In designed catalysts,2D stack structure as an adaptive material can buffer the shock of electrolyte convection,hydrogen bubble rupture,and evolution through the release of stress,which insure the long cycle stability.Meanwhile,the rich porosity between stacked units contributed the good infiltration of electrolyte and slippage of hydrogen bubbles,guaranteeing electrolyte fast recharge and bubble evolution at the high-current catalysis.Beyond that,the electron structure modulation induced by interfacial charge transfer is also beneficial to enhance the intrinsic activity.Profoundly,the multiscale coordinated regulation will provide a guide to design high-efficiency industrial electrocatalysts. | Shucong Zhang Wenbin Wang Feilong Hu Yan Mi Shuzhe Wang Youwen Liu Xiaomeng Ai Jiakun Fang Huiqiao Li Tianyou Zhai | 2020 | Nano-Micro Letters2020，12，10： | 3 |
| 5 | Palladium-Catalyzed Formal [4+2] Cycloaddition of Benzoic and Acrylic Acids with 1,3-Dienes via C-H Bond Activation: Efficient Access to 3,4-Dihydroisocoumarin and 5,6-Dihydrocoumalins显示文摘 | Youwen Sun Guozhu Zhang | 2018 | Chinese Journal of Chemistry2018，36，8： | 3 |
| 6 | Mechanism of dopamine binding and allosteric modulation of the human D1 dopamine receptor显示文摘Dear Editor,Dopamine acts as an essential neurotransmitter whose signaling is conducted through five G protein-coupled receptors(GPCRs),dopamine D1 to D5 receptors(DRD1–DRD5).1 The D1-like receptors,comprising DRD1 and DRD5,primarily couple to the Gs family of G proteins to activate adenylyl cyclase and induce cAMP production.DRD1 is the most abundantly expressed dopamine receptor in the CNS.1 It is the central receptor mediating excitatory dopamine signaling in multiple dopaminergic pathways.Dysregulation of DRD1 signaling has been directly linked to Parkinson’s disease(PD),schizophrenia,and drug abuse. | Youwen Zhuang Brian Krumm Huibing Zhang X.Edward Zhou Yue Wang Xi-Ping Huang Yongfeng Liu Xi Cheng Yi Jiang Hualiang Jiang Cheng Zhang Wei Yi Bryan L.Roth Yan Zhang H.Eric Xu | 2021 | Cell Research2021，31，5： | 2 |
| 7 | Polymyxin resistance caused by large-scale genomic inversion due to IS26 intramolecular translocation in Klebsiella pneumoniae显示文摘Polymyxin B and polymyxin E(colistin)are presently considered the last line of defense against human infections caused by multidrug-resistant Gram-negative organisms such as carbapenemase-producer Enterobacterales,Acinetobacter baumannii,and Klebsiella pneumoniae.Yet resistance to this last-line drugs is a major public health threat and is rapidly increasing.Polymyxin S2(S2)is a polymyxin B analogue previously synthesized in our institute with obviously high antibacterial activity and lower toxicity than polymyxin B and colistin.To predict the possible resistant mechanism of S2for wide clinical application,we experimentally induced bacterial resistant mutants and studied the preliminary resistance mechanisms.Mut-S,a resistant mutant of K.pneumoniae ATCC BAA-2146(Kpn2146)induced by S2,was analyzed by whole genome sequencing,transcriptomics,mass spectrometry and complementation experiment.Surprisingly,large-scale genomic inversion(LSGI)of approximately 1.1 Mbp in the chromosome caused by IS26mediated intramolecular transposition was found in Mut-S,which led to mgrB truncation,lipid A modification and hence S2resistance.The resistance can be complemented by plasmid carrying intact mgrB.The same mechanism was also found in polymyxin B and colistin induced drug-resistant mutants of Kpn2146(Mut-B and Mut-E,respectively).This is the first report of polymyxin resistance caused by IS26 intramolecular transposition mediated mgrB truncation in chromosome in K.pneumoniae.The findings broaden our scope of knowledge for polymyxin resistance and enriched our understanding of how bacteria can manage to survive in the presence of antibiotics. | Haibin Li Lang Sun Han Qiao Zongti Sun Penghe Wang Chunyang Xie Xinxin Hu Tongying Nie Xinyi Yang Guoqing Li Youwen Zhang Xiukun Wang Zhuorong Li Jiandong Jiang Congran Li Xuefu You | 2023 | Acta Pharmaceutica Sinica B2023，13，9： | 1 |
| 8 | Opti-mization of permanent magnet guideway for HTS maglevvehicle with numerical methods 显示文摘 | Zhang Jianghua Zeng Youwen Cheng Jun | 2008 | IEEE Transactionson Applied Superconductivity2008，18，3： | 1 |
| 9 | A Block Parallel l_0-Norm Penalized Shrinkage and Widely Linear Affine Projection Algorithm for Adaptive Filter显示文摘To improve the identification capability of AP algorithm in time-varying sparse system, we propose a block parallel l_0-SWL-DCD-AP algorithm in this paper. In the proposed algorithm, we first introduce the l_0-norm constraint to promote its application for sparse system. Second, we use the shrinkage denoising method to improve its track ability. Third, we adopt the widely linear processing to take advantage of the non-circular properties of communication signals. Last, to reduce the high computational complexity and make it easy to implemented, we utilize the dichotomous coordinate descent(DCD) iterations and the parallel processing to deal with the tapweight update in the proposed algorithm. To verify the convergence condition of the proposed algorithm, we also analyze its steadystate behavior. Several simulation are done and results show that the proposed algorithm can achieve a faster convergence speed and a lower steady-state misalignment than similar APA-type algorithm. When apply the proposed algorithm in the decision feedback equalizer(DFE), the bite error rate(BER) decreases obviously. | Youwen Zhang Shuang Xiao Lu Liu Dajun Sun | 2017 | China Communications2017，14，1： | 1 |
| 10 | Molten-salt synthesis of porous La0.6Sr0.4Co0.2Fe0.8O2.9 perovskite as an efficient electrocatalyst for oxygen evolution显示文摘 | Sanzhao Song Jing Zhou Shuo Zhang Linjuan Zhang Jiong Li Yu Wang Ling Han Youwen Long Zhiwei Hu Jian-Oiang Wang | 2018 | Nano Research2018，11，9： | 1 |
| 11 | Applied Surface Science显示文摘 | Xu Junming Zhang Xiaobin Chen Fei Li Ting Li Yu Tao Xinyong Wang Youwen Wu Xiaojuan | 2005 | 2392005，，： | 1 |
| 12 | Effect of As doping on the photoluminescence of nanocrystalline 74 Ge embedded in SiO 2 matrix显示文摘 | Shaobo Dun Tiecheng Lu Youwen Hu Qiang Hu Liuqi Yu Zheng Li Ningkang Huang Songbao Zhang Bin Tang Junlong Dai Lev Resnick Issai Shlimak | 2008 | Journal of Luminescence2008，，8： | 1 |
| 13 | The Raman spectroscopy of neutron transmutation doping isotope 74 Germanium nanocrystals embedded in SiO 2 matrix显示文摘 | Youwen Hu Tiecheng Lu Shaobo Dun Qiang Hu Ningkang Huang Songbao Zhang Bin Tang Junlong Dai Lev Resnick Issai Shlimak Sha Zhu Qiangmin Wei Lumin Wang | 2006 | Solid State Communications2006，，9： | 1 |
| 14 | The extraeellular matrix protein mindin is a pattern-recognition molecule for microbial pathogens 显示文摘 | YouWen He Li H Zhang J | 2004 | Nat Immunol2004，5，1： | 1 |
| 15 | Spatiotemporal variation and source analysis of air pollutants in the Harbin-Changchun (HC) region of China during 2014e2020显示文摘This study analyzed the characteristics of air pollution and specific pollution periods within the HarbinChangchun(HC)metropolitan area during 2014e2020.Regarding annual,seasonal,and monthly variations of the six pollutants,the change trend in 11 cities of HC showed strong consistency in spatial distribution.The western cities(Songyuan,Daqing,and Siping)were vulnerable to dust storms from Inner Mongolia.The concentrations of all air pollutants,except O3-8h,showed downward fluctuation trends from 2014 to 2018 and remained stable from 2018 to 2020 in terms of annual variations.The interannual trend of significant reductions in SO2 and SO2/PM2.5 during the heating period indicated that strict emission reduction measures posed by the government were highly successful.The ratio of PM2.5/SO2 was used to identify open biomass burning(OBB),which showed a double peak(OctobereNovember(OcteNov),MarcheApril(MareApr)).The burning prohibition shifted the OBB from OcteNov to Mar eApr. | Yulong Wang Youwen Sun Zhiqing Zhang Yuan Cheng | 2021 | Environmental Science and Ecotechnology2021，，4： | 1 |
| 16 | Locally differentially private distributed algorithms for set intersection and union显示文摘Dear editor,Privacy-preserving distributed set intersection and union(PPSI, PPSU) have received much attention in recent years because of their wide applications. Most of existing solutions [1, 2] utilize secure multiparty computation protocols(SMCP) [3, 4] to settle the problem, but the SMCP methods are expensive in computation and communication. Even worse, most SMCP methods hardly continue to work if some participants disconnect. | Qiao XUE Youwen ZHU Jian WANG Xingxin LI Ji ZHANG | 2021 | Science China(Information Sciences)2021，64，11： | 1 |
| 17 | Imprinted functionalized silica sol-gel for solid-phase extraction of tri- azolamin显示文摘 | Guoyou Jin Baofei Zhang Youwen Tang | 2011 | Talanta2011，，84： | 1 |
| 18 | Structural Relief System Construction in China Based on the Experience of the USA显示文摘The USA has already established the perfect structural relief system framework with perfect related research and legislative and judicial practice. Compared with the USA,the structural relief system is far from perfection on the legislative level and the judicial level.With the implementing of Centralized Examination Methods for Operators and Temporary Provisions of Concentrated Assets and Business Stripping of Operators,the structural relief system in China has been preliminarily established. However,from the technical level,microcosmic element pillar system related to stripping system has not been established yet. According to the experience in law enforcement in the USA,China shall also formulate Handbook on Implementing Relief System in the Antitrust Scrutiny of Operators to provide helpful guide for the structural relief system in China. | Liu Fuhua Zhang Youwen | 2014 | 学术界2014，，5： | 1 |
| 19 | 2021皮肤红斑狼疮的诊断、治疗和长期管理指南显示文摘皮肤红斑狼疮(CLE)是一种炎症性自身免疫性疾病,其分型较多,包括急性、亚急性、慢性和间歇性CLE。其中,慢性CLE可以进一步分为红斑狼疮(LE)的几个亚型,如盘状LE、疣状LE、深在性LE、冻疮样LE和Blaschko线状LE等。为了给皮肤科医生和风湿科医生提供CLE的诊断、治疗和长期管理的实用指导建议,本证据和共识指南根据卫生保健实践指南的报告条目(国际RIGHT工作组)目录编制,并在国际实践指南注册平台注册。在亚洲皮肤科学会(ADA)、亚洲皮肤病性病学会(AADV)以及中华医学会皮肤性病学分会(CSD)红斑狼疮研究中心的共同努力下,来自亚洲、美洲、欧洲等16个国家或地区的25名皮肤科医生、7名风湿科医生、1名红斑狼疮领域专家和2名方法学家参与了本指南的制定。所有建议均取得了32名医生中至少80%的同意。作为共识,CLE的诊断主要基于临床和组织病理学表现的评估,并且通过对全身多系统损伤的评估,排除系统性红斑狼疮(SLE)。外用糖皮质激素和钙调磷酸酶抑制剂是局部CLE的一线治疗。对于泛发或严重的CLE皮损和/或局部耐药的病例,可以合并全身治疗,包括抗疟药和/或短期全身使用糖皮质激素。值得注意的是,抗疟药是所有类型CLE的一线全身治疗药物,同样可用于妊娠期患者和儿童。二线治疗用药包括沙利度胺、类维生素A类药物、氨苯砜和甲氨蝶呤,而霉酚酸酯是三线治疗用药。最后,脉冲染料激光或手术可作为四线治疗,用于局部以及发生在不可修饰部位的慢性、难治性CLE皮损。贝利尤单抗亦可作为四线治疗用药,用于活动性SLE患者的泛发性CLE皮损或糖皮质激素逐渐减量期间复发的急性CLE。对于疾病的管理,患者教育和长期随访是必要的。建议在每次随访时适当评估疾病活动度、皮肤和其他器官损伤、生活质量、合并症及可能发生的不良事件。 | Qianjin Lu Hai Long Steven Chow Syarief Hidayat Retno Danarti Yulianto Listiawan Danqi Deng Qing Guo Hong Fang Juan Tao Ming Zhao Leihong Xiang Nan Che Fen Li Hongjun Zhao Chak Sing Lau Fong Cheng Ip King Man Ho Arnelfa C.Paliza Chan Vicheth Kiran Godse Soyun Cho Chew Swee Seow Yoshiki Miyachi Tran Hau Khang Rataporn Ungpakorn Hassan Galadari Rashmikant Shah Kehu Yang Youwen Zhou Carlo Selmi Amr HSawalha Xuan Zhang Yaolong Chen Chrang-Shi Lin 姚文鑫 | 2022 | 中国合理用药探索2022，19，10： | 1 |
| 20 | Fabrication and optical property of single-crystalline InSb nanowire arrays显示文摘 | Youwen Yang Liang Li Xiaohu Huang Guanghai Li Lide Zhang | 2007 | Journal of Materials Science2007，，8： | 1 |
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