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| 1 | Mechanisms of activation and inhibition of Zika virus NS2B-NS3 protease显示文摘 | Xia Chen Kailin Yang Chen Wu Cheng Chen Can Hu Olga Buzovetsky Zefang Wang Xiaoyun Ji Yong Xiong Haitao Yang | 2016 | Cell Research2016，26，11： | 10 |
| 2 | Prokaryotic expression, purification, and polyclonal antibody production of a hydrophobin from Grifolafrondosa显示文摘Hydrophobins 是小真菌的蛋白质自我装配自发地在 hydrophilichydrophobic 接口和变化他们属于的表面的极的性质。新 hydrophobin 基因 hgfI 最近从可食的蘑菇 Grifola frondosa 被识别。在这份报纸,克隆,表示,纯化,和 HGFI 的 polyclonal 抗体准备被描述。hgfI 基因在 EcoRI 和 NdeI 限制地点被克隆进 pET-28a 表示 plasmid 然后转变了成 Escherichia coli BL21 紧张。SDS 页分析证明那 recombinant HGFI 蛋白质被优化集中和 IPTG 的正式就职时间令人满意地表示。因为它的包括身体在传统的处理方法是不可溶解的,表示 recombinant HGFI 蛋白质被 electroelution 净化。在有 Sephadex G-25 的一个脱盐的过程以后, recombinant HGFI 蛋白质被用来使跟随标准协议的成年兔子免疫。ELISA 和西方的污点分析显示生产抗血清能检测表示在的两 HGFI 蛋白质原核生物(E。coli ) 并且在真核细胞的房间(G。frondosa ) 。而且,抗血清被用来在 G 决定 HGFI 蛋白质的本地化。用一种 immunofluorescence 技术的 frondosa 房间。结果证明那 HGFI 蛋白质在房间墙中是局部性的,特别在 hypha 的开始发育的位置。对 HGFI 的 polyclonal 抗体将便于进一步的生产和 HGFI 蛋白质的功能的学习。 | Zefang Wang Shuren Feng Yujian Huang Mingqiang Qiao Baohua Zhang Haijin Xu | 2010 | Acta Biochimica et Biophysica Sinica2010，42，6： | 5 |
| 3 | Structural basis of Zika virus helicase in recognizing its substrates显示文摘Zika 的最近的爆炸爆发病毒(ZIKV ) 感染在南方和中美洲和加勒比海被报导了。与 ZIKV 感染联系的新生的头小畸型已经引起了国际担心的一种公共健康紧急情况。没有特定的疫苗或药当前是可得到的治疗 ZIKV 感染。ZIKV helicase,在病毒的 RNA 复制起一个枢轴的作用,是为治疗的一个吸引人的目标。我们决定了 ZIKV helicase-ATP-Mn 2+ 和 ZIKV helicase-RNA 的水晶结构。这是到 ATP 的任何 flavivirus helicase 界限的第一结构。有相关 flavivirus helicases 的比较证明了尽管在活跃地点的批评 P 环在不同种类之中有可变符合构造,认出 ATP/Mn 2+ 采用一个相同模式。ZIKV helicase-RNA 的结构表明了在 RNA 绑定之上,马达领域的旋转能引起重要 conformational 变化。惊人地,尽管 ZIKV 和登革热病毒(DENV ) apo-helicases 份额为 RNA 绑定保存了残余,他们在为 RNA 识别的不同单个模式的马达域旋转结果的不同礼貌。它建议 flavivirus helicases 能发展了一台保存引擎为 RNA 放松,而是不同马达领域旋转从 nucleoside triphosphate 把化学能变换成机械精力在可变 RNA 识别的结果适应单个病毒的复制的模式。 | Hongliang Tian Xiaoyun Ji Xiaoyun Yang Zhongxin Zhang zuoku Lu Kailin Yang Cheng Chen Qi Zhao Heng Chi Zhongyu Mu Wei Xie Zefang Wang Huiqiang Lou Haitao Yang Zihe Rao | 2016 | Protein & Cell2016，7，8： | 3 |
| 4 | Issues and rational design of aqueous electrolyte for Zn-ion batteries显示文摘Aqueous Zn-ion batteries(AZIBs)are regarded as a promising alternative to the widely used lithium-ion batteries in large-scale energy storage systems.The researches on the development of novel aqueous electrolyte to improve battery performance have also attracted great interest since the electrolyte is a key com-ponent for Zn2+migration between cathode and anode.Herein,we briefly sum-marized and illuminated the recent development tendency of aqueous electrolyte for AZIBs,then deeply analyzed its existing issues(water decomposition,cathode dissolution,corrosion and passivation,and dendrite growth)and discussed the corresponding optimization strategies(pH regulation,concentrated salt solution,electrolyte composition design,and functional additives).The internal mecha-nisms of these strategies were further revealed and the relationships between issues and solutions were clarified,which could guide the future development of aqueous electrolytes for AZIBs. | Qi Zhang Zefang Yang Huimin Ji Xiaohui Zeng Yougen Tang Dan Sun Haiyan Wang | 2021 | SusMat2021，1，3： | 3 |
| 5 | Numerical Study on the Leakage and Diffusion Characteristics of Low-Solubility and Low-Volatile Dangerous Chemicals from Ship in Inland Rivers显示文摘Considering the accidents of ships for dangerous chemicals transportation in inland rivers,a numerical method for the simulation of the leakage and diffusion processes of dangerous chemicals in inland rivers is proposed in this paper.Geographic information,such as rivers and buildings in the model,is obtained through Google Earth and structures of rivers and buildings are described by Auto CAD.In addition,the Fluent is adopted to simulate the leakage and diffusion processes of the dangerous chemicals where the standard k-εmodel is used to calculate the turbulent flow.Considering the interaction between chemicals and water,the VOF method is used to describe the leakage,drift and diffusion process of dangerous chemicals groups on the water surface.Taking a section of the Yangtze River as an example,the leakage and diffusion processes from a ship carrying 3,000 tons of low-solubility and low-volatile dangerous chemicals are studied,and the characteristics of leakage and diffusion are analyzed in detail.During the simulation,the area of the maximum group of leaked dangerous chemicals reaches up to about 1800 m2,and the number reaches up to 45.Furthermore,the influence of density,viscosity,water velocity and leakage velocity on the leakage and diffusion processes is investigated in this paper. | Shuifen Zhan Mingchao Wang Min Wang Qianqian Shao Zefang Zhang Wenxin Jiang Xuemin Chen | 2020 | Computer Modeling in Engineering & Sciences2020，，4： | 2 |
| 6 | Intrinsically zincophobic protective layer for dendrite-free zinc metal anode显示文摘Aqueous zinc anodes have attracted the attention of many researchers owing to their high safety,low cost,and high theoretical specific capacity.However,its practical application is severely limited by the dendrite growth on zinc anode.Herein,we develop an intrinsically zincophobic barium-titanate protective layer with a porous structure to suppress the zinc dendrite formation by homogenizing the ion distribution on the anode surface,increasing the nucleation sites,and limiting the irregular zinc growth.Based on these synergistic effects,the coated zinc anode can exhibit long cycle life(840 h at 0.5 mA/cm^(2) for 0.5 mAh/cm^(2))and low voltage hysteresis(36 mV).This work can provide a feasible direction for the design of intrinsically zincophobic coating materials to uniformize the zinc stripping and plating. | Chunlin Xie Qi Zhang Zefang Yang Huimin Ji Yihu Li Huanhuan Li Liang Fu Dan Huang Yougen Tang Haiyan Wang | 2022 | Chinese Chemical Letters2022，33，5： | 1 |
| 7 | Terahertz Spectroscopy for Accurate Identification of Panax quinquefolium Basing on Nonconjugated 24(R)-Pseudoginsenoside F_(11)显示文摘Panax quinquefolium is a perennial herbaceous plant that contains many beneficial ginsenosides with diverse pharmacological effects.24(R)-pseudoginsenoside F_(11)is specific to P.quinquefolium,a useful biomarker for distinguishing this species from other related plants.However,because of its nonconjugated property and the complexity of existing detection methods,this biomarker cannot be used as the identification standard.We herein present a stable 24(R)-pseudoginsenoside F_(11)fingerprint spectrum in the terahertz band,thereby proving that F_(11)can be detected and quantitatively analyzed via terahertz spectroscopy.We also analyzed the sample by high-performance liquid chromatography-triple quadrupole mass spectrometry.The difference between the normalized data for the two analytical methods was less than 5%.Furthermore,P.quinquefolium from different areas and other substances can be clearly distinguished based on these terahertz spectra with a standard principal component analysis.Our method is a fast,simple,and cost-effective approach for identifying and quantitatively analyzing P.quinquefolium. | Tianyi Kou Ji Ye Jing Wang Yan Peng Zefang Wang Chenjun Shi Xu Wu Xitian Hu Haihong Chen Ling Zhang Xiaohong Chen Yiming Zhu Huiliang Li Songlin Zhuang | 2021 | Plant Phenomics2021，3，1： | 1 |
| 8 | Preparation of monodisperse polystyrene/silica core–shell nano-composite abrasive with controllable size and its chemical mechanical polishing performance on copper显示文摘 | Lei Zhang Haibo Wang Zefang Zhang Fei Qin Weili Liu Zhitang Song | 2011 | Applied Surface Science2011，，3： | 1 |
| 9 | Distributed reinforcement learning based framework for energy-efficient UAV relay against jamming显示文摘Unmanned aerial vehicle(UAV)network is vulnerable to jamming attacks,which may cause severe damage like communication outages.Due to the energy constraint,the source UAV cannot blindly enlarge the transmit power,along with the complex network topology with high mobility,which makes the destination UAV unable to evade the jammer by flying at will.To maintain communication with a limited battery capacity in the UAV networks in the presence of a greedy jammer,in this paper,we propose a distributed reinforcement learning(RL)based energy-efficient framework for the UAV networks with constrained energy under jamming attacks to improve the communication quality while minimizing the total energy consumption of the network.This framework enables each relay UAV to independently select its transmit power based on historical state-related information without knowing the moving trajectory of other UAVs as well as the jammer.The location and battery level of each UAV need not be shared with other UAVs.We also propose a deep RL based anti-jamming relay approach for UAVs with portable computation equipment like Raspberry Pi to achieve higher and faster performance.We study the Nash equilibrium(NE)and the performance bounds based on the formulated power control game.Simulation results show that the proposed schemes can reduce the bit error rate(BER)and reduce energy consumption of the UAV network compared with the benchmark method. | Weihang Wang Zefang Lv Xiaozhen Lu Yi Zhang Liang Xiao | 2021 | Intelligent and Converged Networks2021，2，2： | 1 |
| 10 | Role of TGF-β1 and its Receptors in Breast Carcinogenesis:Evaluation of Gene Expression Patterns and Clinical Implications显示文摘OBJECTIVE Transforming growth factor β1 (TGF-β1) is a multifunc-tional cytokine that may play an important role in tumor development and progression. METHODS We evaluated gene expression patterns of TGF-β1 and its receptors [transforming growth factor β type I receptor (TβR-Ⅰ) and transforming growth factor β type II receptor (TβR-Ⅱ)] in tumor tissue from patients with breast cancer or with benign breast diseases (BBD) and adja-cent normal tissue from the patients with breast cancer. Included in the study were 527 breast cancer patients and 213 BBD patients who participated in the Shanghai Breast Cancer Study. RESULTS The expression levels of the TGF-β1, TβR-Ⅰand TβR-Ⅱ genes in breast tissue were quantified using real-time PCR. TβR-Ⅱ expres-sion in cancer tissue was decreased by over 50% as compared to either adjacent normal tissue from the same patients or benign tumor tissue from BBD patients (p<0.001). TGF-β1 expression was lower by approximately 20% in cancer tissue compared to adjacent normal tissue (p=0.14) or to be-nign tumor tissue (p=0.002). Although TβR-Ⅰ expression was also reduced in cancer tissue compared to adjacent normal tissue, or benign tumor tissue, the magnitude of the reduction was less apparent than that for TβR-Ⅱ. Compared to patients with the lowest tertile value for TβR-Ⅱ, patients with median tertile value for TβR-Ⅱ had more favorable overall survival (HR 0.47, 95% CI 0.27-0.85) and disease-free survival (HR 0.65, 95% CI 0.39-1.06). No apparent associations, however, were observed between TGF-β1 or TβR-Ⅰ expression and overall or disease-free survival. CONCLUSION The results from this study support the hypothesis that a decreased level of TβR-Ⅱ gene expression, and thus reduced TGF-β1 sensitivity, is related to breast tumor progression. | Wenjing Wang Aesun Shin Qiuyin Cai Zefang Ren Xiao-Ou Shu Yutang Gao Harold I. Moses Wei Lu Wei Zheng | 2007 | Chinese Journal of Clinical Oncology2007，4，3： | 1 |
| 11 | Structural insight into the Zika virus capsid encapsulating the viral genome显示文摘 | Ting Li Qi Zhao Xiaoyun Yang Cheng Chen Kailin Yang Chen Wu Tianqing Zhang Yinkai Duan Xiaoyu Xue Kaixia Mi Xiaoyun Ji Zefang Wang Haitao Yang | 2018 | Cell Research2018，28，4： | 0 |
| 12 | Carbazole and tetrahydro-carboline derivatives as dopamine D_(3) receptor antagonists with the multiple antipsychotic-like properties显示文摘Dopamine D_(3) receptor(D_(3)R)is implicated in multiple psychotic symptoms.Increasing the D_(3)R selectivity over dopamine D_2 receptor(D_2R)would facilitate the antipsychotic treatments.Herein,novel carbazole and tetrahydro-carboline derivatives were reported as D_(3)R selective ligands.Through a structure-based virtual screen,ZLG-25(D_(3)R K_i=685 nmol/L;D_2R K_i>10,000 nmol/L)was identified as a novel D_(3)R selective bitopic ligand with a carbazole scaffold.Scaffolds hopping led to the discovery of novel D_(3)R-selective analogs with tetrahydro-β-carboline or tetrahydro-γ-carboline core.Further functional studies showed that most derivatives acted as h D_(3)R-selective antagonists.Several lead compounds could dose-dependently inhibit the MK-801-induced hyperactivity.Additional investigation revealed that 23j and 36b could decrease the apomorphine-induced climbing without cataleptic reaction.Furthermore,36b demonstrated unusual antidepressant-like activity in the forced swimming tests and the tail suspension tests,and alleviated the MK-801-induced disruption of novel object recognition in mice.Additionally,preliminary studies confirmed the favorable PK/PD profiles,no weight gain and limited serum prolactin levels in mice.These results revealed that 36b provided potential opportunities to new antipsychotic drugs with the multiple antipsychotic-like properties. | Zhongtang Li Fan Fang Yiyan Li Xuehui Lv Ruqiu Zheng Peili Jiao Yuxi Wang Guiwang Zhu Zefang Jin Xiangqing Xu Yinli Qiu Guisen Zhang Zhongjun Li Zhenming Liu Liangren Zhang | 2023 | Acta Pharmaceutica Sinica B2023，13，11： | 0 |
| 13 | Functionalizing the interfacial double layer to enable uniform zinc deposition显示文摘Due to the unsatisfactory electrode/electrolyte interface,the metallic Zn dendrites and corrosion are easily induced,severely hindering the applications of zinc-ion batteries(ZIBs).Herein,a strategy that engineers the interfacial double layer by an extremely low concentration of sulfolane is proposed to tune the Zn stripping/plating behavior.It is revealed that the highly-polar sulfolane can predominately occupy the inner Helmholtz layer over water,and then regulate the upcoming Zn2+to directly deposit downward.Simultaneously,the widened Helmholtz layer can weaken the electric field intensity,which will generate more nucleation sites and reduce the nuclei radius,thereby promoting uniform zinc deposition as well.Moreover,corrosion byproducts can be inhibited since fewer water molecules can contact the Zn electrodes.Consequently,the battery performance can be naturally optimized.With an optimum amount of sulfolane,the Zn||Zn battery can operate for more than 1,100 h under1 m A cm^(-2)and 1 m Ah cm^(-2).And the as-constructed Zn||NaV_(3)O_(8)·1.5H_(2)O battery demonstrates considerably higher cycling stability than that without sulfolane.Overall,this work has provided a deep insight into constructing a functional interfacial double layer to regulate zinc deposition,which can also act as a reference for other metal-based batteries. | Yihu Li Hao Wang Tingqing Wu Chunlin Xie Zefang Yang Qi Zhang Dan Sun Yougen Tang Liang Fu Haiyan Wang | 2023 | Science China Chemistry2023，66，6： | 0 |
| 14 | Designing Zinc Deposition Substrate with Fully Preferred Orientation to Elude the Interfacial Inhomogeneous Dendrite Growth显示文摘The development of zinc-ion batteries with high energy density remains a great challenge due to the uncontrollable dendrite growth on their zinc metal anodes.Film anodes plated on the substrate have attracted increasing attention to alleviate these dendrite issues.Herein,we first point out that both the random crystal orientation and the low metal affinity of the substrate are important factors of zinc dendrite formation.Accordingly,the(1 O 1)fully preferred tin interface layer with high zinc affinity was fabricated by chemical tin plating on(1 O o)oriented copper.This tin decorated copper substrate can realize high reversible zinc plating/stripping behavior,and full cell using this zinc plated substrate can be operated for more than 1000 cycles with high capacity retention(85.3%)and low electrochemical impedance.The proposed strategy can be also applied to lithium metal batteries,which demonstrates that the substrate orientation regulation and metal affinity design are the promising approaches to achieve dendrite-free metal anode and overcome the challenges of highly reactive metal anodes. | Chunlin Xie Zefang Yang Qi Zhang Huimin Ji Yihu Li Tingqing Wu Wenbin Li Pengfei Wu Haiyan Wang | 2023 | Research2023，，1： | 0 |
| 15 | Encapsulation of polyethylene glycol in cellulose-based porous capsules for latent heat storage and light-to-thermal conversion显示文摘Phase change materials are potential candidates for the application of latent heat storage.Herein,we fabricated porous capsules as shape-stable materials from cellulose-based polyelectrolyte complex,which were first prepared using cellulose 6-(N-pyridinium)hexanoyl ester as the cationic polyelectrolyte and carboxymethyl cellulose as the anionic polyelectrolyte to encapsulate polyethylene glycol by the vacuum impregnation method.Furthermore,the multi-walled carbon nanotube or graphene oxide,which were separately composited into the polyelectrolytes complex capsules to enhance thermal conductivity and light-to-thermal conversion efficiency.These capsules owned a typical core–shell structure,with an extremely high polyethylene glycol loading up to 34.33 g∙g^(‒1).After loading of polyethylene glycol,the resulted cellulose-based composite phase change materials exhibited high thermal energy storage ability with the latent heat up to 142.2 J∙g^(‒1),which was 98.5%of pure polyethylene glycol.Further results showed that the composite phase change materials demonstrated good form-stable property and thermal stability.Moreover,studies involving light-to-thermal conversion determined that composite phase change materials exhibited outstanding light-to-thermal conversion performance.Considering their exceptional comprehensive features,innovative composite phase change materials generated from cellulose presented a highly interesting choice for thermal management and renewable thermal energy storage. | Jiangwei Li Lina Meng Jiaxuan Chen Xu Chen Yonggui Wang Zefang Xiao Haigang Wang Daxin Liang Yanjun Xie | 2023 | Frontiers of Chemical Science and Engineering2023，17，8： | 0 |
| 16 | Traffic accidents of autonomous vehicles based on knowledge mapping:A review显示文摘As a characteristic representative of the new generation of vehicles,autonomous driving is expected to improve people's driving experience.The study on traffic accident of autonomous vehicles(AVs)helps provide suggestions for autonomous driving safety from multiple disciplines and perspectives,and provide support for formulating traffic accident treatment schemes.Knowledge mapping,as a cutting-edge research method in bibliometrics,scientifically and objectively displays the relevant research status using visual means.This paper uses CiteSpace 6.1.r3 to analyze 5068 related literature on the Web of Science database from 1991 to 2022 and finds out major thematic clusters,important documents and representative journals according to citation frequency.The results show that research on traffic accidents involving AVs focuses on accident preventing technologies,including how to avoid collisions,track lane-position,and enhance vehicle-toeverything(V2X)communication.This paper extracts the mean research topics and key points involved in the field and illustrates journals related to AVs and traffic accidents,which provides guidance for subsequent researchers to carry out in-depth research and contribute their papers.Popular journals are in disciplines of mathematics,systems,computer,economics,and social science.This paper also suggests scholars to consider the aspects of scene reconstruction,cause analysis,and injury of vulnerable road users,so as to investigate traffic accidents and put forward effective treatment schemes to reduce AV accidents,and ultimately improve road safety. | Wei Ji Quan Yuan Gang Cheng Shengnan Yu Min Wang Zefang Shen Tiantong Yang | 2023 | Journal of Traffic and Transportation Engineering(English Edition)2023，10，6： | 0 |
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