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| 1 | 小麦TILLING突变体检测中基因组DNA提取方法的优化显示文摘为了优化小麦高通量TILLING突变体扫描所需基因组DNA提取的方法,对利用SDS法、改良SDS法、CTAB法、改良CTAB法、AxyPrep DNA提取试剂盒、Qiagen DNeasy Plant Mini Kit试剂盒以及再生Qiagen硅胶柱方法提取的小麦基因组DNA进行了系统比较。结果表明,尽管7种方法所提取DNA的纯度及琼脂糖凝胶电泳检测结果均良好,但再生Qiagen硅胶柱方法、CTAB法和改良SDS法的DNA浓度显著高于其他方法。根据小麦细胞分裂素氧化酶基因1(TaCKX1)的DNA序列设计引物,以新鲜DNA样品用于PCR扩增大于1 000bp的片段时,后4种方法扩增效果优于前3种方法;DNA在-20℃下保存100d后重新进行PCR扩增时,则只有Qiagen试剂盒和再生Qiagen硅胶柱2种方法提取的DNA能扩增出大于1 000bp的片段。采用本实验室与新西兰坎特伯雷大学合作建立的再生硅胶柱与自配提取液提取小麦基因组DNA,既保证了DNA的高质量,又降低了使用试剂盒的成本,为小麦TILLING库的构建及其他相关试验提供了一种经济有效的DNA提取方法。 | 徐艳 徐继法 陈磊 赵吉强 FU Zeyu JAMESON Paula E 宋建成 | 2017 | 麦类作物学报2017，37，2： | 8 |
| 2 | On the relay selec- tion for cooperative wireless networks with physical-layer network coding显示文摘 | Zeyu Zhcng Shengli Fu Kejie Lu | 2012 | Wireless Network2012，18，6： | 1 |
| 3 | Numerical simulation and response analysis of microspherical focused logging in inclined micro-fractured formation显示文摘Tight and unconventional reservoirs have become the focus with the progress of petroleum exploration and development.Micro-fractures in these reservoirs can effectively improve reservoir permeability,and well-developed micro-fractures can serve to directly improve productivity.Compared with the centered electrical well logging method,the Micro Spherical Focused Logging(MSFL)is more suitable for microfracture identification due to its high resolution and near borehole wall measuring method.In this study,an anisotropic model is used to depict micro-fractured formation.First,a forward model with microfractured formation,borehole,logging instrument and surrounding rock is established.Subsequently,MSFL responses under different micro-fracture porosity,resistivity,dip angle and borehole radius,are calculated based on the finite element method(FEM).Finally,the MSFL responses under different microfracture parameters are analyzed with the response laws clarified. | Nan Zeyu Fu Weishu Liu Zhiyuan | 2023 | Energy Geoscience2023，4，2： | 0 |
| 4 | Study on Influencing Factors of Mental Health of Mobile Young White-Collar Workers in China显示文摘Mobile young white-collar workers not only have the characteristics of mobile young people,but also have the characteristics of general white-collar workers.Under the influence of both,their mental health may be suffering from“double disadvantage”.So,based on an ecological model of the stress process,this paper tries to use the data of the questionnaire on the mental health of mobile young white-collar workers in Zhejiang Province to explore the influence of some factors in the middle workplace and residence place on the mental health of micro individuals.The results show that:(1)The working environment with high control and low freedom and the workplace discrimination against the mobile status will have a negative impact on the mental health of mobile young white-collar workers;(2)Financial anxiety in daily life will lead to a decline in the mental health level of mobile young white-collar workers;(3)Good organizational support and neighborhood social relations can significantly relieve life pressure,so as to effectively improve the mental health of mobile young white-collar workers.It can be seen that we also need to pay more attention to the mental health of mobile young white-collar workers in order to improve their situation. | Tao Liu Lin Liu Zeyu Chen Rong Fu | 2024 | International Journal of Mental Health Promotion2024，26，2： | 0 |
| 5 | Small-molecule agents for cancer immunotherapy显示文摘Cancer immunotherapy,exemplified by the remarkable clinical benefits of the immune checkpoint blockade and chimeric antigen receptor T-cell therapy,is revolutionizing cancer therapy.They induce long-term tumor regression and overall survival benefit in many types of cancer.With the advances in our knowledge about the tumor immune microenvironment,remarkable progress has been made in the development of small-molecule drugs for immunotherapy.Small molecules targeting PRR-associated pathways,immune checkpoints,oncogenic signaling,metabolic pathways,cytokine/chemokine signaling,and immune-related kinases have been extensively investigated.Monotherapy of smallmolecule immunotherapeutic drugs and their combinations with other antitumor modalities are under active clinical investigations to overcome immune tolerance and circumvent immune checkpoint inhibitor resistance.Here,we review the latest development of small-molecule agents for cancer immunotherapy by targeting defined pathways and highlighting their progress in recent clinical investigations. | Fang Wang Kai Fu Yujue Wang Can Pan Xueping Wang Zeyu Liu Chuan Yang Ying Zheng Xiaopeng Li Yu Lu Kenneth Kin Wah To Chenglai Xia Jianye Zhang Zhi Shi Zeping Hu Min Huang Liwu Fu | 2024 | Acta Pharmaceutica Sinica B2024，14，3： | 0 |
| 6 | Effects of sediment characteristics on the sediment transport capacity of overland flow显示文摘The transport of sediments is a crucial part of soil erosion.Accurately calculating the sediment transport capacity is key to the construction of soil erosion process models.Research on Tc has focused mainly on the dynamics of a single particle of sediment and hydraulic variables.There have been few studies of the impact of soil aggregates on the Tc.To clarify how sediment characteristics,including those for single particles and aggregates,affect the Tc of overland flow with no raindrop import,flume experiments were implemented at slope gradients varying from 5.24%to 26.80%and flow discharges ranging from 0.68 to 5.41×10^(-3)m^(2)s^(-1).The experimental materials were five typical soils in China.The results indicated that the correlation between the measured Tc and sediment mechanical composition indexes of the five soils was indistinctive in this study.The sediment settling velocity with aggregates has a significant corre-lation with the measured Tc.New equations,including for the sediment settling velocity with aggregatesωud75,were established to calculate the Tc.The empirical equation that includedωud75,slope gradient and unit discharge performed greatly in predicting Tc(R^(2)=0.93,NSE=0.90).ωud75 can effectively improve the calculation accuracy of Tc.The new equation including flow and sediment properties obtained through dimensional analysis performed well in predicting Tc(R^(2)=0.99,NSE=0.91),and the calculation accuracy was better than that of the empirical model derived in this study.These findings indicate that the sediment settling velocity is an important variable in the equation for predicting sediment transport capacity of overland flow. | Chenguang Liu Suhua Fu Zhanbin Li Zeyu Zhang Jianhui Zeng | 2023 | International Soil and Water Conservation Research2023，11，1： | 0 |
| 7 | Luminescence and recombine centre in ZnO/Si films显示文摘The D0h luminescence of ZnO films deposited on p-type Si substrates is produced by metal-organic chemical vapor deposition(MOCVD).After annealing in the air at 700°C for an hour,the photoluminescence(PL)spectra,the I-V characteristics and the deep level transient spectroscopy(DLTS)of the samples are measured.All the samples have a rectification characteristic.DLTS signals show two deep levels of E1 and E2.The Gaussian fit curves of the PL spectra at room temperature show three luminescence lines b,c and d,of which b is attributed to the exciton emission.The donor level E1 measured by DLTS and the location state donor ionization energy Ed of the closely neighboring emission lines c and d are correlated.E1 is judged as neutral donor bound to hole emission(D0h).Moreover,the intensity of the PL spectra decreases while the relative density of E2 increases,showing that E2 has the property of a nonradiative center. | Cihui LIU Ran YAO Jianfeng SU Zeyu MA Zhuxi FU | 2009 | Frontiers of Electrical and Electronic Engineering in China2009，4，1： | 0 |
| 8 | Development and challenges of cells-and materials-based tooth regeneration显示文摘Tooth defect and loss are common clinical diseases in stomatology.With the extension of life expectancy,there is an increasing demand for tooth tissue and whole tooth regeneration.Compared with traditional oral prosthetic treatment,tooth regeneration has unique advantages and has become one of the hotspots towards oral biomedical treatment.In this review,we discussed the development and challenges of tooth regeneration based on cells and materials,including tooth enamel,dentin,dental pulp,cementum,dentin-pulp complex,and the whole tooth regeneration,in order to provide a comprehensive,up-to-date,illustrative overview of tooth regeneration issues.The mechanisms of the regeneration were also summarized and discussed.Moreover,this review hints the future perspective and research direction of tooth regeneration in the challenging field of regenerative dentistry. | Zeyu Fu Yu Zhuang Jinjie Cui Ruilong Sheng Helena Tomás João Rodrigues Bin Zhao Xudong Wang Kaili Lin | 2022 | Engineered Regeneration2022，3，2： | 0 |
| 9 | A survey of emergency blunt urethral injury management in China:Reality versus guidelines显示文摘Background:Although urethral trauma may lead to serious consequences if mismanaged,treatment concepts are inconsistent.We designed a survey to investigate the current diagnosis and management of emergency blunt urethral trauma to aid future dissemination of relevant concepts.Methods:A 15‐item anonymous questionnaire was distributed via an online platform.It addressed items such as the cognition of how to diagnose of urethral trauma,optimal emergency management of a urethral trauma patient,and attitude towards early realignment for pelvic fracture urethral injuries.Results:Of 538 respondents,94.2%and 84.9%had received patients with straddle trauma urethral injuries or pelvic fracture urethral injuries,respectively,within the past year.In the emergency room,attempted urethral catheterization was the most selected examination method by respondents for diagnosis of both straddle injury(500/538)and pelvic fracture urethral injury(469/538).For patients with straddle injury,41.3%of respondents performed endoscopic realignment and 31.6%preferred suprapubic cystostomy.For hemodynamically stable patients with PFUI,42.2%of respondents preferred suprapubic cystostomy and 34.9%preferred endoscopic realignment.Most respondents felt favorably toward early realignment for pelvic fracture urethral injuries.After realignment,61.3%,24.5%,and 13.8%of respondents performed catheterization for 4,8,and 12 weeks,respectively.Further,54.6%of respondents believed catheter traction should be applied after realignment.Conclusion:Although the number of yearly emergency urethral trauma cases was small,the opinions and practices of most urologists were consistent with guidelines.However,the significance of retrograde urethrography was not fully understood,and some respondents had incorrect views on catheter traction after realignment. | Yubo Gu Changhao Hou Jiahao Lin Wei Yuan Zeyu Wang Xianjie Xiu Qiang Fu Lujie Song | 2023 | UroPrecision2023，1，2： | 0 |
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