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| 1 | Washed microbiota transplantation vs.manual fecal microbiota transplantation:clinical findings,animal studies and in vitro screening显示文摘Fecal microbiota transplantation(FMT)by manual preparation has been applied to treat diseases for thousands of years.However,this method still endures safety risks and challenges the psychological endurance and acceptance of doctors,patients and donors.Population evidence showed the washed microbiota preparation with microfiltration based on an automatic purification system followed by repeated centrifugation plus suspension for three times significantly reduced FMT-related adverse events.This washing preparation makes delivering a precise dose of the enriched microbiota feasible,instead of using the weight of stool.Intraperitoneal injection in mice with the fecal microbiota supernatant obtained after repeated centrifugation plus suspension for three times induced less toxic reaction than that by the first centrifugation following the microfiltration.The toxic reactions that include death,the change in the level of peripheral white blood cells,and the proliferation of germinal center in secondary lymphoid follicles in spleen were noted.The metagenomic next-generation sequencing(NGS)indicated the increasing types and amount of viruses could be washed out during the washing process.Metabolomics analysis indicated metabolites with pro-inflammatory effects in the fecal microbiota supernatant such as leukotriene B4,corticosterone,and prostaglandin G2 could be removed by repeated washing.Near-infrared absorption spectroscopy could be served as a rapid detection method to control the quality of the washingprocess.In conclusion,this study for the first time provides evidence linking clinical findings and animal experiments to support that washed microbiota transplantation(WMT)is safer,more precise and more quality-controllable than the crude FMT by manual. | Ting Zhang Gaochen Lu Zhe Zhao Yafei Liu Quan Shen Pan Li Yaoyao Chen Haoran Yin Huiquan Wang Cicilia Marcella Bota Cui Lei Cheng Guozhong Ji Faming Zhang | 2020 | Protein & Cell2020，11，4： | 44 |
| 2 | Effect of Boschniakia rossica on expression of GSTP,p53 and p21^(ras)proteins in early stage of chemical hepatocarcinogenesis and its anti-inflammatory activities in rats显示文摘AIM To investigate the effect of Boschniakiarossica(BR)extract on expression of GST-P,p53 and p21rasproteins in early stage of chemicalhepatocarcinogenesis in rats and its anti-inflammatory activities.METHODS The expression of tumor marker-placental form glutathione S-transferase(GST-P),p53 and p21rasproteins were investigated byimmunohistochemical techniques and ABCmethod.Anti-inflammatory activities of BR werestudied by xylene and croton oil-induced mouseear edema,carrageenin,histamine and hotscald-induced rat pow edema,adjuvant-inducedrat arthritis and cotton pellet-induced mousegranuloma formation methods.RESULTS The 500 mg/kg of BR-H2O extractfractionated from BR-Methanol extract hadinhibitory effect on the formation of DEN-inducedGST-P-positive foci in rat liver(GST-P stainingwas 78% positive in DEN+AAF group vs 20%positive in DEN+AAF+BR group,P<0.05)andthe expression of mutant p53 and p21rasproteinwas lower than that of hepatic preneoplasticlesions(33% and 22% positive respectively inDEN+AAF group vs negative in DEN+AAF+BRgroup).Both CH2Cl2 and H2O extracts from BRhad anti-inflamatory effect in xylene and crotonoil-induced mouse ear edema(inhibitory rateswere 26%-29% and 35%-59%,respectively). BR-H2O extract exhibited inhibitory effect incarrageenin,histamine and hot scald-inducedhind paw edema and adjuvant-induced arthritis inrats and cotton pellet-induced granulomaformation in mice.CONCLUSION BR extract exhibited inhibitory effect on formation of preneoplastic hepatic foci in early stage of rat chemical hepato-carcinogenesis. Both CH2CI2 and H2O extracts from BR exerted anti-inflammatory effect in rats and mice. | Zong Zhu Yin Hai Ling Jin Xue Zhe Yin Tian Zhu Li Ji Shu Quan Zeng Nan Jin Institute for Cancer Research,Yanbian University College of Medicine,Yanji 133000,Jilin Province,China | 2000 | World Journal of Gastroenterology2000，6，6： | 33 |
| 3 | Carbonyl emissions from heavy-duty diesel vehicle exhaust in China and the contribution to ozone formation potential显示文摘Fifteen heavy-duty diesel vehicles were tested on chassis dynamometer by using typical heavy duty driving cycle and fuel economy cycle. The air from the exhaust was sampled by 2,4-dinitrophenyhydrazine cartridge and 23 carbonyl compounds were analyzed by high performance liquid chromatography. The average emission factor of carbonyls was 97.2 mg/km, higher than that of light-duty diesel vehicles and gasoline-powered vehicles. Formaldehyde, acetaldehyde, acetone and propionaldehyde were the species with the highest emission factors. Main influencing factors for carbonyl emissions were vehicle type, average speed and regulated emission standard, and the impact of vehicle loading was not evident in this study. National emission of carbonyls from diesel vehicles exhaust was calculated for China, 2011, based on both vehicle miles traveled and fuel consumption. Carbonyl emission of diesel vehicle was estimated to be 45.8 Gg, and was comparable to gasolinepowered vehicles(58.4 Gg). The emissions of formaldehyde, acetaldehyde and acetone were 12.6, 6.9, 3.8 Gg, respectively. The ozone formation potential of carbonyls from diesel vehicles exhaust was 537 mg O3 /km, higher than 497 mg O3 /km of none-methane hydrocarbons emitted from diesel vehicles. | Dong Dong Min Shao Yue Li Sihua Lu Yanjun Wang Zhe Ji Dagang Tang | 2014 | Journal of Environmental Sciences2014，26，1： | 20 |
| 4 | N6-Methyladenine DNA Methylation in Japonica and Indica Rice Genomes and Its Association with Gene Expression,Plant Development, and Stress Responses显示文摘N6-Methyladenine (6mA)DNA methylation has recently been implicated as a potential new epigenetic marker in eukaryotes,including the dioot modelArabidopsis thaliana.However,the conservation and divergence of 6mA distribution patterns and functions in plants remain elusive.Here we report high-quality 6mA methylomes at single-nucleotide resolution in rice based on substantially improved genome sequences of two rice cultivars,Nipponbare (Nip;Japonica)and 93-11 (Indica).Analysis of 6mA genomic distribution and its association with transcription suggest that 6mA distribution and function is rather conserved between rice and Arabidopsis.We found that 6mA levels are positively correlated with the expression of key stressrelated genes,which may be responsible for the difference in stress tolerance between Nip and 93-11. Moreover,we showed that mutations in DDM1 cause defects in plant growth and decreased 6mA level. Our results reveal that 6mA is a conserved DNA modification that is positively associated with gene expression and contributes to key agronomic traits in plants. | Qian Zhang Zhe Liang Xuean Cui Changmian Ji Yun Li Pingxian Zhang Jingrong Liu Adeel Riaz Pu Yao Min Liu Yunpeng Wang Tiegang Lu Hao Yu Donglei Yang Hongkun Zheng Xiaofeng Gu | 2018 | Molecular Plant2018，11，12： | 18 |
| 5 | Advanced Space-based Solar Observatory(ASO-S):an overview显示文摘The Advanced Space-based Solar Observatory(ASO-S)is a mission proposed for the 25 th solar maximum by the Chinese solar community.The scientific objectives are to study the relationships between the solar magnetic field,solar flares and coronal mass ejections(CMEs).Three payloads are deployed:the Full-disk vector Magneto Graph(FMG),the Lyman-αSolar Telescope(LST)and the Hard X-ray Imager(HXI).ASO-S will perform the first simultaneous observations of the photospheric vector magnetic field,non-thermal imaging of solar flares,and the initiation and early propagation of CMEs on a single platform.ASO-S is scheduled to be launched into a 720 km Sun-synchronous orbit in 2022.This paper presents an overview of the mission till the end of Phase-B and the beginning of Phase-C. | Wei-Qun Gan Cheng Zhu Yuan-Yong Deng Hui Li Yang Su Hai-Ying Zhang Bo Chen Zhe Zhang Jian Wu Lei Deng Yu Huang Jian-Feng Yang Ji-Jun Cui Jin Chang Chi Wang Ji Wu Zeng-Shan Yin Wen Chen Cheng Fang Yi-Hua Yan Jun Lin Wei-Ming Xiong Bin Chen Hai-Chao Bao Cai-Xia Cao Yan-Ping Bai Tao Wang Bing-Long Chen Xin-Yu Li Ye Zhang Li Feng Jiang-Tao Su Ying Li Wei Chen You-Ping Li Ying-Na Su Hai-Yan Wu Mei Gu Lei Huang Xue-Jun Tang | 2019 | Research in Astronomy and Astrophysics2019，19，11： | 17 |
| 6 | Breakdown Properties of Nano-SiO2/LDPE Composite显示文摘聚合物纳米复合介质的击穿强度与纳米填充颗粒的质量分数、粒径和表面处理密切相关。以不同表面处理的纳米SiO2颗粒为填料,制备了不同填充质量分数、粒径的纳米SiO2/低密度聚乙烯(low density polyethylene,LDPE)复合介质,测试了其在交流、直流正极性和直流负极性3种不同类型电场下的击穿场强。结果表明:在所研究范围内,填充纳米SiO2颗粒可以提高低密度聚乙烯的击穿场强,并且随着填充质量分数的增加,复合介质的击穿场强有升高的趋势。纳米颗粒填充质量分数相同时,在12~16am粒径下,复合介质的击穿场强有随纳米SiO2粒径的升高而降低的趋势;在7nm小粒径下,可能由于纳米颗粒容易团聚,导致击穿场强较粒径大时要低。另外,纳米SiO2颗粒表面经疏水性处理后,能够有效提高复合介质的击穿场强。 | LAN Li WU Jiandong JI Zheqiang WANG Qiaohua LI Zhe XIAO Dengming YIN Yi | 2012 | 中国电机工程学报2012，32，13： | 14 |
| 7 | A new tumor necrosis factor(TNF)-α regulator,lipopolysaccharides-induced TNF-α factor,is associated with obesity and insulin resistance显示文摘背景肿瘤坏死因素( TNF )-在调停起一个重要作用在肥胖和相关 disorders.Lipopolysaccharides ( LPS )的煽动性的状态导致了TNF-因素( LITAF )最近作为TNF-和另外的煽动性的 cytokines 的一个管理者被验证,并且因为这研究的一个 transcriptional factor.The 目的是证实在 LITAF 和肥胖和胰岛素 resistance.Methods 之间的协会,也许行动有大量身体团索引( BMI )的 47 个题目是 included.Subjects | JI Zhen-zhong DAI Zhe XU Yan-cheng | 2011 | Chinese Medical Journal2011，，2： | 9 |
| 8 | Allele-defined genome reveals biallelic differentiation during cassava evolution显示文摘Dear Editor,Generation of heterozygous genomes by hybridization between or within species can help maintain plant diversity and serve as a potential source of new species(Baek et al.,2018).Moreover,genomic heterozygosity is associated with genomic coadaptation,developmental stability,and heterosis.Accurate definition of alleles in haplotypes is necessary to precisely characterize allelic variation controlling agriculturally important traits(Shi et al.,2019).Currently,most released genomes have mosaic assembly of haplotypes due to random selection or collapse of alleles during genome assembly(Shi et al.,2019),which masked allelic variation and functional differentiation of divergent alleles in heterozygous species. | Wei Hu Changmian Ji Haitao shi Zhe Liang Zehong Ding Jianqiu Ye Wenjun Ou Gang Zhou Weiwei Tie Yan Yan Jinghao Yang Xiaoying Yang Yunxie Wei Zhiqiang Jin Jianghui Xie Ming Peng Wenquan Wang Anping Guo Biyu Xu Jianchun Guo Songbi Chen Liming Ma Mingcheng Wang Yang Zhou Xiaolong Li Ruoxi Li Siyi Guo Xinhui Xiao Zhongqing Wan Feifei An Jie Zhang Qingyun Leng Yin Li Ray Ming Kaimian Li | 2021 | Molecular Plant2021，14，6： | 8 |
| 9 | Whole genome sequencing of 10K patients with acute ischaemic stroke or transient ischaemic attack: design, methods and baseline patient characteristics显示文摘Background and purpose Stroke is the second leading cause of death worldwide and the leading cause of mortality and long-term disability in China,but its underlying risk genes and pathways are far from being comprehensively understood.We here describe the design and methods of whole genome sequencing(WGS)for 10914 patients with acute ischaemic stroke or transient ischaemic attack from the Third China National Stroke Registry(CNSR-III).Methods Baseline clinical characteristics of the included patients in this study were reported.DNA was extracted from white blood cells of participants.Libraries are constructed using qualified DNA,and WGS is conducted on BGISEQ-500 platform.The average depth is intended to be greater than 30×for each subject.Afterwards,Sentieon software is applied to process the sequencing data under the Genome Analysis Toolkit best practice guidance to call genotypes of single nucleotide variants(SNVs)and insertion-deletions.For each included subject,21 fingerprint SNVs are genotyped by MassARRAY assays to verify that DNA sample and sequencing data originate from the same individual.The copy number variations and structural variations are also called for each patient.All of the genetic variants are annotated and predicted by bioinformatics software or by reviewing public databases.Results The average age of the included 10914 patients was 62.2±11.3 years,and 31.4%patients were women.Most of the baseline clinical characteristics of the 10914 and the excluded patients were balanced.Conclusions The WGS data together with abundant clinical and imaging data of CNSR-III could provide opportunity to elucidate the molecular mechanisms and discover novel therapeutic targets for stroke. | Si Cheng Zhe Xu Yang Liu Jinxi Lin Yong Jiang Yilong Wang Xia Meng Anxin Wang Xinying Huang Zhimin Wang Guohua Chen Songdi Wu Zhengchang Jia Yongming Chen Xuerong Qiu Jun Wu Binbin Song Weizhong Ji Zhongping An Wenjun Xue Lili Zhao Yu Geng Hongyan Li Hao Li Yongjun Wang | 2021 | Stroke & Vascular Neurology2021，6，2： | 8 |
| 10 | XELOX doublet regimen versus EOX triplet regimen as first-line treatment for advanced gastric cancer:An open-labeled,multicenter,randomized,prospective phase III trial(EXELOX)显示文摘Background:There is no consensus on whether triplet regimen is better than doublet regimen in the first-line treatment of advanced gastric cancer(AGC).We aimed to compare the efficacy and safety of oxaliplatin plus capecitabine(XELOX)and epirubicin,oxaliplatin,plus capecitabine(EOX)regimens in treating AGC.Methods:This phase III trial enrolled previously untreated patients with AGC whowere randomly assigned to receive the XELOXor EOXregimen.The primary endpoint was non-inferiority in progression-free survival(PFS)for XELOX as compared with EOX on an intention-to-treat basis.Results:Between April 10,2015 andAugust 20,2020,448AGCpatientswere randomized to receive XELOX(n=222)or EOX(n=226).The median PFS(mPFS)was 5.0 months(95%confidence interval[CI]=4.5-6.0 months)in the XELOX arm and 5.5 months(95%CI=5.0-6.0 months)in the EOX arm(hazard ratio[HR]=0.989,95%CI=0.812-1.203;P_(non-inferiority)=0.003).There was no significant difference inmedian overall survival(mOS)(12.0 vs.12.0months,P=0.384)or objective response rate(37.4%vs.45.1%,P=0.291)between the two groups.In patients with poorly differentiated adenocarcinoma and liver metastasis,the EOX arm had a significantly longer mOS(P=0.021)and a trend of longer mPFS(P=0.073)than the XELOX arm.The rate of grade 3/4 adverse events(AEs)was 42.2%(90/213)in the XELOX arm and 72.5%(156/215)in the EOX arm(P=0.001).The global health-related quality of life(QoL)score was significantly higher in the XELOX arm than in the EOX arm during chemotherapy.Conclusions:This non-inferiority trial demonstrated that the doublet regimen was as effective as the triplet regimen and had a better safety profile and QoL as a first-line treatment for AGC patients.However,the triplet regimen might have a survival advantage in patients with poorly differentiated adenocarcinoma and liver metastasis. | Xiao-Dong Zhu Ming-Zhu Huang Yu-Sheng Wang Wan-Jing Feng Zhi-Yu Chen Yi-Fu He Xiao-Wei Zhang Xin Liu Chen-Chen Wang Wen Zhang Jie-Er Ying Jun Wu Lei Yang Yan-Ru Qin Jian-Feng Luo Xiao-Ying Zhao Wen-Hua Li Zhe Zhang Li-Xin Qiu Qi-Rong Geng Jian-Ling Zou Jie-Yun Zhang Hong Zheng Xue-Feng Song Shu-Sheng Wu Cheng-Yan Zhang Zhe Gong Qin-Qin Liu Xiao-Feng Wang Qi Xu Qi Wang Jian-Mei Ji Jian Zhao Wei-Jian Guo | 2022 | Cancer Communications2022，42，4： | 6 |
| 11 | Epigallocatechin-3-gallate induces apoptosis, inhibits proliferation and decreases invasion of glioma cell显示文摘Epigallocatechin-3-gallate (EGCG), a major polyphenol in green tea, has been considered a potential therapeutic and chemopreventive agent for cancer. Glioma is a malignant tumor with high mortality but effective therapy has not yet been developed. In this study, we found that EGCG induced apoptosis in U251 glioma cells via the laminin receptor (molecular weight 67kDa) in a time- and dose-dependent manner, decreased their invasiveness and inhibited their proliferation. The mitogen-activated protein kinase pathway was shown to be involved in glioma cell apoptosis and proliferation. Furthermore, the mRNA levels of matrix metalloproteinase (MMP)-2 and MMP-9 were reduced after EGCG treatment. These results suggest that EGCG has important therapeutic effects with low toxicity and side-effects, and could be used in cancer chemoprevention. | Hong Li Zhe Li Ya-Ming Xu Yue Wu Kang-Kang Yu Can Zhang Yong-Hua Ji Gang Ding Fu-Xue Chen | 2014 | Neuroscience Bulletin2014，30，1： | 6 |
| 12 | Emission characteristics of offshore fishing ships in the Yellow Bo Sea, China显示文摘Maritime transport has been playing a decisive role in global trade. Its contribution to the air pollution of the sea and coastal areas has been widely recognized. The air pollutant emission inventories of several harbors in China have already been established. However,the emission factors of local ships have not been addressed comprehensively, and thus are lacking from the emission inventories. In this study, on-board emission tests of eight diesel-powered offshore fishing ships were conducted near the coastal region of the northern Yellow Bo Sea fishing ground of Dalian, China. Results show that large amounts of fine particles(< 0.5 μm, 90%) were found in maneuvering mode, which were about five times higher than those during cruise mode. Emission rates as well as emission factors based on both distance and fuel were determined during the cruise and maneuvering modes(including departure and arrival). Average emission rates and distance-based emission factors of CO, HC and PM were much higher during the maneuvering mode as compared with the cruise mode. However, the average emission rate of Nitrous Oxide(NOx) was higher during the cruise mode as compared with the maneuvering modes. On the contrary, the average distance-based emission factors of NOxwere lower during the cruise mode relative to the maneuvering mode due to the low sailing speed of the latter. | Yingshuai Liu Yunshan Ge Jianwei Tan Mingliang Fu Asad Naeem Shah Luqiang Li Zhe Ji Yan Ding | 2018 | Journal of Environmental Sciences2018，30，3： | 5 |
| 13 | Research progress of photocatalysis based on highly dispersed titanium in mesoporous SiO_2显示文摘With the development of the human economy and green chemistry, people pay much more attention to environmental safety. Correspondingly, mesoporous TiO_2 and its correlated photocatalysts are able to help people seek for better life. In this review, first of all, we briefly introduce the preparations and applications of mesoporous TiO_2-SiO_2 materials, which exhibit excellent performance in pollutants decomposition and H_2 evolution in photocatalysis. Then, we review the mesoporous composites of TiSiO_2 materials, which are ideal materials used in the photoreduction of air pollutants such as CO_2, NO and NO_2. It is powerfully evident from the literature surveys that these TiO_2 based mesoporous photocatalysts possess a large potential in environment and energy development. | Chencheng Dong Jiahui Ji Zhe Yang Yifei Xiao Mingyang Xing Jinlong Zhang | 2019 | Chinese Chemical Letters2019，30，4： | 5 |
| 14 | HEMOPERFUSION IN THE MANAGEMENT OF PATIENTS SEVERELY POISONED BY ORGANOPHOSPHORUS显示文摘 | Mao Yuying XuZhougyi(Zhu ji people’s Hosptal,Zhe jiang province,Chine) | 1997 | Chinese Journal of Biomedical Engineering(English Edition)1997，6，4： | 5 |
| 15 | Genetic divergence of weedy rice populations associated with their geographic location and coexisting conspecific crop: Implications on adaptive evolution of agricultural weeds显示文摘蔓延的米饭,栽培米饭的一样的生物种类,是大批出没于世界范围的稻田的一棵有害杂草。在中国决定蔓延的米饭的基因差异,结构,和关系,我们使用了插入 / 删除(InDel ) 分析蔓延的米饭人口从的分子的指纹对作为参考书种区域,使用的装饰用的梨树和 indica 米饭栽培变种的南部的米饭东北。InDel 指纹显示了相对高的全面基因差异(H 为从 14 张蔓延的米饭人口的 240 件样品的 e = 0.42) 。然而,许多更低的在内人口差异被检测,特别地为从东北、南部的中国的人口,与 H e 价值从 0.006 ~ 0.06 。江苏人口显示出高得多的在内人口基因差异(H e = 0.12-0.31) 比那些从另外的区域。分子的变化和 F st 显示出 ∼88% 在蔓延的米饭人口之中的基因变化。主要部件和结构分析显示了实质的 japonica-indica 蔓延的米饭人口的基因区别。从江苏省的蔓延的米饭甚至在人口以内正在经历 indica 装饰用的梨树区别,它由于由在这个区域的装饰用的梨树米饭变化的 indica 的代替是可能的。在结论,重要基因分叉在中国发生在蔓延的米饭人口之中,它与他们的地理地点和共存的米饭变化被联系。从栽培米饭的基因渗入在在人影响的 agro 生态系统塑造蔓延的米饭人口的基因差异和结构有一个关键角色。 | Zhuo-Jun songI Zhe Wang Yang Feng Nan Yao Ji Yang Bao-Rong Lu | 2015 | Journal of Systematics and Evolution2015，53，4： | 5 |
| 16 | Using side-dressing technique to reduce nitrogen leaching and improve nitrogen recovery efficiency under an irrigated rice system in the upper reaches of Yellow River Basin, Northwest China显示文摘The excessive nitrogen(N) fertilizer input coupled with flood irrigation might result in higher N leaching and lower nitrogen recovery efficiency(NRE).Under an intensive rice system in the Ningxia irrigation region,China,environmental friendly N management practices are heavily needed to balance the amount of N input for optimum crop production while minimize the nitrogen loss.The objective of this study was to determine the influences of side-dressing(SD) technique in mechanical transplanting systems on the NRE,N leaching losses and rice yield in anthropogenic-alluvial soil during two rice growing seasons(2010-2011).Four fertilizer N treatments were established,including conventional urea rate(CU,300 kg ha^(-1)yr^(-1));higher SD of controlled-release N fertilizer rate(SD1,176 kg ha^(-1)yr^(-1));lower SD of controlled-release N fertilizer rate(SD2,125 kg ha^(-1)yr^(-1));and control(CK,no N fertilizer).Field lysimeters were used to quantify drainage from undisturbed soil during six rice growing stages.Meanwhile,the temporal variations of total nitrigen(TN),NO_3^--N,and NH_4^+-N concentrations in percolation water were examined.The results showed that SD1 substantially improved NRE and reduced N leaching losses while maintaining rice yields.Across two years,the averaged NRE under SD1 treatment increased by 25.5%as relative to CU,but yet the rice yield was similar between two treatments.On average,the nitrogen loss defined as TN,NH_4^+-N,and NO_3^--N underthe SD1 treatment reduced by 27.4,37.2 and 24.1%,respectively,when compared with CU during the study periods.Although the SD2 treatment could further reduce N leaching loss to some extent,this technique would sharply decline rice yield,with the magnitude of as high as 21.0%relative to CU treatment.Additionally,the average NRE under SD2 was 11.2% lower than that under SD1 treatment.Overall,the present study concluded that the SD technique is an effective strategy to reduce N leaching and increase NRE,thus potentially mitigate local environmental threat.We propose SD1 as a novel alternative fertilizer technique under an irrigated rice-based system in Ningxia irrigation region when higher yields are under consideration. | ZHANG Ai-ping GAO Ji LIU Ru-liang ZHANG Qing-wen CHEN Zhe YANG Shi-qi YANG Zheng-li | 2016 | Journal of Integrative Agriculture2016，15，1： | 5 |
| 17 | Activation of CaMKIIδA promotes Ca^2+ leak from the sarcoplasmic reticulum in cardiomyocytes of chronic heart failure rats显示文摘 | Le GUI Xin GUO Zhe ZHANG Hui XU Ya-wei JI Ren-jun WANG Jiang-hua ZHU Qing-hui CHEN | 2018 | Acta Pharmacologica Sinica2018，39，10： | 5 |
| 18 | CircRNA-SORE mediates sorafenib resistance in hepatocellular carcinoma by stabilizing YBX1显示文摘Sorafenib is the first-line chemotherapeutic therapy for advanced hepatocellular carcinoma(HCC).However,sorafenib resistance significantly limits its therapeutic efficacy,and the mechanisms underlying resistance have not been fully clarified.Here we report that a circular RNA,circRNA-SORE(a circular RNA upregulated in sorafenib-resistant HCC cells),plays a significant role in sorafenib resistance in HCC.We found that circRNA-SORE is upregulated in sorafenib-resistant HCC cells and depletion of circRNA-SORE substantially increases the cell-killing ability of sorafenib.Further studies revealed that circRNA-SORE binds the master oncogenic protein YBX1 in the cytoplasm,which prevents YBX1 nuclear interaction with the E3 ubiquitin ligase PRP19 and thus blocks PRP19-mediated YBX1 degradation.Moreover,our in vitro and in vivo results suggest that circRNA-SORE is transported by exosomes to spread sorafenib resistance among HCC cells.Using different HCC mouse models,we demonstrated that silencing circRNA-SORE by injection of siRNA could substantially overcome sorafenib resistance.Our study provides a proof-of-concept demonstration for a potential strategy to overcome sorafenib resistance in HCC patients by targeting circRNA-SORE or YBX1. | Junjie Xu Lin Ji Yuelong Liang Zhe Wan Wei Zheng Xiaomin Song Kirill Gorshkov Qiming Sun Hui Lin Xueyong Zheng Jiang Chen Ren-an Jin Xiao Liang Xiujun Cai | 2020 | Signal Transduction and Targeted Therapy2020，5，1： | 5 |
| 19 | Genome-wide identification of agronomically important genes in outcrossing crops using OutcrossSeq显示文摘Many important crops(e.g.,tuber,root,and tree crops)are cross-pollinating.For these crops,no inbred lines are available for genetic study and breeding because they are self-incompatible,clonally propagated,or have a long generation time,making the identification of agronomically important genes difficult,particularly in crops with a complex autopolyploid genome.In this study,we developed a method,OutcrossSeq,for mapping agronomically important loci in outcrossing crops based on whole-genome low-coverage resequencing of a large genetic population,and designed three computation algorithms in OutcrossSeq for different types of outcrossing populations.We applied OutcrossSeq to a tuberous root crop(sweet potato,autopolyploid),a tree crop(walnut tree,highly heterozygous diploid),and hybrid crops(double-cross populations)to generate high-density genotype maps for the outcrossing populations,which enable precise identification of genomic loci underlying important agronomic traits.Candidate causative genes at these loci were detected based on functional clues.Taken together,our results indicate that OutcrossSeq is a robust and powerful method for identifying agronomically important genes in heterozygous species,including polyploids,in a cost-efficient way.The OutcrossSeq software and its instruction manual are available for downloading at www.xhhuanglab.cn/tool/OutcrossSeq.html. | Mengjiao Chen Weijuan Fan Feiyang Ji Hua Hua Jie Liu Mengxiao Yan Qingguo Ma Jiongjiong Fan Qin Wang Shufeng Zhang Guiling Liu Zhe Sun Changgeng Tian Fengling Zhao Jianli Zheng Qi Zhang Jiaxin Chen Jie Qiu Xin Wei Ziru Chen Peng Zhang Dong Pei Jun Yang Xuehui Huang | 2021 | Molecular Plant2021，14，4： | 4 |
| 20 | Seasonal variations of soil bacterial communities in Suaeda wetland of Shuangtaizi River estuary, Northeast China显示文摘Estuarine wetland is the transitional interface linking terrestrial with marine ecosystems,and wetland microbes are crucial to the biogeochemical cycles of nutrients.The soil samples were collected in four seasons(spring,S1;summer,S2;autumn,S3;and winter,S4)from Suaeda wetland of Shuangtaizi River estuary,Northeast China,and the variations of bacterial community were evaluated by high-throughput sequencing.Soil properties presented a significant seasonal change,including p H,carbon(C)and total nitrogen(TN),and the microbial diversity,richness and structure also differed with seasons.Canonical correspondence analysis(CCA)and Mantel tests implied that soil p H,C and TN were the key factors structuring the microbial community.Gillisia(belonging to Bacteroidetes)and Woeseia(affiliating with Gammaproteobacteria)were the two primary components in the rhizosphere soils,displaying opposite variations with seasons.Based on PICRUSt(Phylogenetic Investigation of Communities by Reconstruction of Unobserved States)prediction,the xenobiotics biodegradation related genes exhibited a seasonal decline,while the majority of biomarker genes involved in nitrogen cycle showed an ascending trend.These findings could advance the understanding of rhizosphere microbiota of Suaeda in estuarine wetland. | Xuwang Zhang Zhe Ji Yating Shao Chaochen Guo Hao Zhou Lifen Liu Yuanyuan Qu | 2020 | Journal of Environmental Sciences2020，32，11： | 4 |
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