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| 1 | Exogenous plant MIR168a specifically targets mammalian LDLRAPI: evidence of cross-kingdom regulation by microRNA显示文摘 | Lin Zhang Dongxia Hou Xi Chen Donghai Li Lingyun Zhu Yuj ing Zhang Jing Li Zhen Bian Xiangying Liang Xing Cai Yuan Yin Cheng Wang Tianfu Zhang Dihan Zhu Dianmu Zhang Jie Xu Qun Chen Yi Ba Jing Liu Qiang Wang Jianqun Chen Jin Wang Meng Wang Qipeng Zhang Junfeng Zhang Ke Zen Chen-Yu Zhang | 2012 | Cell Research2012，22，1： | 155 |
| 2 | Homology-mediated end joining-based targeted integration using CRISPR/Cas9显示文摘 | Yao, Xuan Wang, Xing Hu, Xinde Liu, Zhen Liu, Junlai Zhou, Haibo Shen, Xiaowen Wei, Yu Huang, Zijian Ying, Wenqin Wang, Yan Nie, Yan-Hong Zhang, Chen-Chen Li, Sanlan Cheng, Leping Wang, Qifang Wu, Yan Huang, Pengyu Sun, Qiang Shi, Linyu Yang, Hui | 2017 | Cell Research2017，27，6： | 28 |
| 3 | Taxus chinensis ameliorates diabetic nephropathy through down-regulating TGF-β1/Smad pathway显示文摘Diabetic nephropathy(DN) is one of the common microvascular complications of diabetes mellitus. Renal fibrosis is closely related to the deterioration of renal function. The present study aimed to investigate protective effect of Taxus chinensis on high-fat diet/streptozotocin-induced DN in rats and explore the underlying mechanism of action. The rat DN model was established via feeding high fat diet for 4 weeks and subsequently injecting streptozotocin(30 mg·kg^(-1) body weight) intraperitoneally. The rats with blood glucose levels higher than 16.8 mmol·L^(-1) were selected for experiments. The DN rats were treated with Taxus chinensis orally(0.32, 0.64, and 1.28 g·kg^(-1)) once a day for 8 weeks. Taxus chinensis significantly improved the renal damage, which was indicated by the decreases in 24-h urinary albumin excretion rate, blood serum creatinine, and blood urea nitrogen. Histopathological examination confirmed the protective effect of Taxus chinensis. The thickness of glomerular basement membrane was reduced, and proliferation of mesangial cells and podocytes cells and increase in mesangial matrix were attenuated. Further experiments showed that Taxus chinensis treatment down-regulated the expression of TGF-β1 and α-SMA, inhibited phosphorylation of Smad2 and Smad3. These results demonstrated that Taxus chinensis alleviated renal injuries in DN rats, which may be associated with suppressing TGF-β1/Smad signaling pathway. | WENG Hong-Bo HAN Wen-Ke XIONG Yan-Wen JIN Zhou-Hui LAN Zhen LIU Cheng ZHANG Xue-Mei PENG Wen | 2018 | Chinese Journal of Natural Medicines2018，16，2： | 28 |
| 4 | BMAL1 knockout macaque monkeys display reduced sleep and psychiatric disorders显示文摘Circadian disruption is a risk factor for metabolic, psychiatric and age-related disorders, and non-human primate models could help to develop therapeutic treatments. Here, we report the generation of BMAL1 knockout cynomolgus monkeys for circadian-related disorders by CRISPR/Cas9 editing of monkey embryos. These monkeys showed higher nocturnal locomotion and reduced sleep, which was further exacerbated by a constant light regimen. Physiological circadian disruption was reflected by the markedly dampened and arrhythmic blood hormonal levels. Furthermore, BMAL1-deficient monkeys exhibited anxiety and depression, consistent with their stably elevated blood cortisol, and defective sensory processing in auditory oddball tests found in schizophrenia patients. Ablation of BMAL1 up-regulated transcriptional programs toward inflammatory and stress responses, with transcription networks associated with human sleep deprivation, major depressive disorders, and aging. Thus, BMAL1 knockout monkeys are potentially useful for studying the physiological consequences of circadian disturbance, and for developing therapies for circadian and psychiatric disorders. | Peiyuan Qiu Jian Jiang Zhen Liu Yijun Cai Tao Huang Yan Wang Qiming Liu Yanhong Nie Fang Liu Jiumu Cheng Qing Li Yun-Chi Tang Mu-ming Poo Qiang Sun Hung-Chun Chang | 2019 | National Science Review2019，6，1： | 21 |
| 5 | One-step generation of complete gene knockout mice and monkeys by CRISPR/Cas9-mediated gene editing with multiple sgRNAs显示文摘CRISPR/Cas9 系统是一个有效编辑基因的方法,但是编辑基因的动物的多数显示出 mosaicism,与编辑仅仅在房间的部分发生。这里,我们证明那单个基因或多重基因能被 Cas9 mRNA 和多重邻近的单个指南的 RNA 的 zygotic 注射完全在老鼠和猴子胚胎击倒(spaced 10-200 bp 分开) 指向仅仅一把单个钥匙每基因的 exon。个别地在 Y 染色体上跟随八基因的指向的删除的 F0 老鼠的 Phenotypic 分析在产生猛烈老鼠表明了这条途径的坚韧性。重要地,这条途径在高效率交付完全的基因大美人(Arntl 上的 100% 并且 91% 在 Prrt2 上) 在猴子胚胎。最后,我们能在一个单个步骤产生一只完全的 Prrt2 猛烈猴子,表明在很快建立编辑基因的猴子模型的这条途径的实用性。 | Erwei Zuo Yi-Jun Cai Kui Li Yu Wei Bang-An Wang Yidi Sun Zhen Liu Jiwei Liu Xinde Hu Wei Wei Xiaona Huo Linyu Shi Cheng Tang Dan Liang Yan Wang Yan-Hong Nie Chen-Chen Zhang Xuan Yao Xing Wang Changyang Zhou Wenqin Ying Qifang Wang Ren-Chao Chen Qi Shen Guo-Liang Xu Jinsong Li Qiang Sun Zhi-Qi Xiong Hui Yang | 2017 | Cell Research2017，27，7： | 21 |
| 6 | Effect of Ce doping of TiO_2 support on NH_3-SCR activity over V_2O_5–WO_3/CeO_2–TiO_2 catalyst显示文摘CeO2–TiO2composite supports with different Ce/Ti molar ratios were prepared by a homogeneous precipitation method, and V2O5–WO3/CeO2–TiO2catalysts for the selective catalytic reduction(SCR) of NOx with NH3 were prepared by an incipient-wetness impregnation method. These catalysts were characterized by means of BET, XRD, UV–Vis,Raman and XPS techniques. The results showed that the catalytic activity of V2O5–WO3/TiO2 was greatly enhanced by Ce doping(molar ratio of Ce/Ti = 1/10) in the TiO2 support.The catalysts that were predominantly anatase TiO2 showed better catalytic performance than the catalysts that were predominantly fluorite CeO2. The Ce additive could enhance the surface adsorbed oxygen and accelerate the SCR reaction. The effects of O2 concentration, ratio of NH3/NO, space velocity and SO2 on the catalytic activity were also investigated. The presence of oxygen played an important role in NO reduction. The optimal ratio of NH3/NO was 1/1 and the catalyst had good resistance to SO2 poisoning. | Kai Cheng Jian Liu Tao Zhang Jianmei Li Zhen Zhao Yuechang Wei Guiyuan Jiang Aijun Duan | 2014 | Journal of Environmental Sciences2014，26，10： | 18 |
| 7 | NH_3-SCR denitration catalyst performance over vanadium–titanium with the addition of Ce and Sb显示文摘Selective catalytic reduction technology using NH3 as a reducing agent(NH3-SCR) is an effective control method to remove nitrogen oxides. TiO2-supported vanadium oxide catalysts with different levels of Ce and Sb modification were prepared by an impregnation method and were characterized by X-ray diffractometer(XRD), Brunauer–Emmett–Teller(BET), Transmission electron microscopy(TEM), Fourier transform infrared spectroscopy(FT-IR), UV–Vis diffuse reflectance spectroscopy(UV–Vis DRS), Raman and Hydrogen temperature-programmed reduction(H2-TPR). The catalytic activities of V5 CexS by/TiO2 catalysts for denitration were investigated in a fixed bed flow microreactor. The results showed that cerium, vanadium and antimony oxide as the active components were well dispersed on TiO2, and the catalysts exhibited a large number of d–d electronic transitions, which were helpful to strengthen SCR reactivity. The V5 CexS by/TiO2 catalysts exhibited a good low temperature NH3-SCR catalytic activity. In the temperature range of 210 to 400℃, the V5 CexS by/TiO2 catalysts gave NO conversion rates above 90%. For the best V5Ce35Sb2/TiO2 catalyst, at a reaction temperature of 210℃, the NO conversion rate had already reached 90%. The catalysts had different catalytic activity with different Ce loadings. With the increase of Ce loading, the NO conversion rate also increased. | Chi Xu Jian Liu Zhen Zhao Fei Yu Kai Cheng Yuechang Wei Aijun Duan Guiyuan Jiang | 2015 | Journal of Environmental Sciences2015，27，5： | 17 |
| 8 | Chinese Hα Solar Explorer(CHASE)——a complementary space mission to the ASO-S显示文摘The Chinese HαSolar Explorer(CHASE)is designed to test a newly developed satellite platform and conduct solar observations.The scientific payload of the satellite is an Hαimaging spectrograph(HIS),which can,for the first time,acquire full-disk spectroscopic solar observations in the Hαwaveband.This paper briefly introduces CHASE/HIS including its scientific objectives,technical parameters,scientific application system,etc.The CHASE mission is scheduled to launch in 2021.It will complement the observations by on-orbit solar spacecraft(such as SDO,IRIS,STEREO and PSP),as well as future solar missions of the Solar Orbiter and Advanced Space-based Solar Observatory(ASO-S). | Chuan Li Cheng Fang Zhen Li Ming-De Ding Peng-Fei Chen Zhe Chen Liang-Kui Lin Chang-Zheng Chen Chang-Ya Chen Hong-Jiang Tao Wei You Qi Hao Yu Dai Xin Cheng Yang Guo Jie Hong Min-Jie An Wei-Qiang Cheng Jian-Xin Chen Wei Wang Wei Zhang | 2019 | Research in Astronomy and Astrophysics2019，19，11： | 12 |
| 9 | Discoloration mechanism,structures and recent applications of thermochromic materials via different methods:A review显示文摘Thermochromic material is a kind of smart material whose color will vary as the result of the phase transition caused by the temperature change. The characteristics of thermochromic materials are the memory functions to the temperature, having great potential applications in aerospace, military, anticounterfeiting technology, construction and other fields. In recent years, many kinds of thermochromic materials have been prepared by different methods and their discoloration mechanisms are various according to published literatures. In this paper, the classification, discoloration mechanism, preparation methods, application fields and development trend of thermochromic materials are reviewed. | Youliang Cheng Xiaoqiang Zhang Changqing Fang Jing Chen Zhen Wang | 2018 | Journal of Materials Science & Technology2018，34，12： | 12 |
| 10 | Biological imaging without autofluorescence in the second near-infrared region显示文摘Fluorescence imaging is capable of acquiring anatomical and functional infor- mation with high spatial and temporal resolution. This imaging technique has been indispensable in biological research and disease detection/diagnosis. Imaging in the visible and to a lesser degree, in the near-infrared (NIR) regions below 900 nm, suffers from autofluorescence arising from endogenous fluorescent molecules in biological tissues. This autofluorescence interferes with fluorescent molecules of interest, causing a high background and low detection sensitivity. Here, we report that fluorescence imaging in the 1,500-1,700-nm region (termed "NIR-IIb") under 808-nm excitation results in nearly zero tissue autofluorescence, allowing for background-free imaging of fluorescent species in otherwise notoriously autofluorescent biological tissues, including liver. Imaging of the intrinsic fluorescence of individual fluorophores, such as a single carbon nanotube, can be readily achieved with high sensitivity and without autofluorescence background in mouse liver within the 1,500-1,700-nm wavelength region. | Shuo Diao Guosong Hong Alexander L. Antaris Jeffrey L. Blackbum Kai Cheng Zhen Cheng Hongjie Dai | 2015 | Nano Research2015，8，9： | 12 |
| 11 | Protective Effect of Resveratrol on the Brain in a Rat Model of Epilepsy显示文摘Accumulating evidence has suggested resveratrol as a promising drug candidate for the treatment of epilepsy. To validate this, we tested the protective effect of resveratrol on a kainic acid(KA)-induced epilepsy model in rats and investigated the underlying mechanism. We found that acute resveratrol application partially inhibited evoked epileptiform discharges in the hippocampal CA1 region. During acute, silent and chronic phases of epilepsy,the expression of hippocampal kainate glutamate receptor(GluK2) and the GABAAreceptor alpha1 subunit(GABAAR-alpha1) was up-regulated and down-regulated,respectively. Resveratrol reversed these effects and induced an antiepileptic effect. Furthermore, in the chronic phase, resveratrol treatment inhibited the KA-induced increased glutamate/GABA ratio in the hippocampus. The antiepileptic effects of resveratrol may be partially attributed to the reduction of glutamate-induced excitotoxicity and the enhancement in GABAergic inhibition. | Zhen Li Zhuyan You Min Li Liang Pang Juan Cheng Liecheng Wang | 2017 | Neuroscience Bulletin2017，33，3： | 11 |
| 12 | Pharmacokinetic Studies of Factor VIII in Chinese Boys with Severe Hemophilia A: A Single-Center Study显示文摘 | Zhen-Ping Chen Pei-jing Li Gang Li Ling Tang Ying-Zi Zhen Xin-Yi Wu Xiao-Ling Cheng Koon Hung Luke Victor S Blanchette Man-Chiu Poon Qiu-Lan Ding Run-Hui Wu | 2018 | Chinese Medical Journal2018，，15： | 11 |
| 13 | A de novo Genome of a Chinese Radish Cultivar显示文摘Here, we report a high-quality draft genome of a Chinese radish(Raphanus sativus) cultivar. This draft contains 387.73 Mb of assembled scaffolds, 83.93% of the scaffolds were anchored onto nine pseudochromosomes and 95.09% of 43 240 protein-coding genes were functionally annotated. 184.75 Mb(47.65%) of repeat sequences was identified in the assembled genome. By comparative analyses of the radish genome against 10 other plant genomes, 2 275 genes in 780 gene families were found unique to R. sativus. This genome is a good reference for genomic study and of great value for genetic improvement of radish. | ZHANG Xiaohui YUE Zhen MEI Shiyong QIU Yang YANG Xinhua CHEN Xiaohua CHENG Feng WU Zhangyan SUN Yuyan JING Yi LIU Bo SHEN Di WANG Haiping CUI Na DUAN Yundan WU Jian WANG Jinglei GAN Caixia WANG Jun WANG Xiaowu LI Xixiang | 2015 | Horticultural Plant Journal2015，1，3： | 10 |
| 14 | SIDT1-dependent absorption in the stomach mediates host uptake of dietary and orally administered microRNAs显示文摘Dietary microRNAs have been shown to be absorbed by mammals and regulate host gene expression,but the absorption mechanism remains unknown.Here,we show that SIDT1 expressed on gastric pit cells in the stomach is required for the absorption of dietary microRNAs.SIDT1-deficient mice show reduced basal levels and impaired dynamic absorption of dietary microRNAs.Notably,we identified the stomach as the primary site for dietary microRNA absorption,which is dramatically attenuated in the stomachs of SIDT1-deficient mice.Mechanistic analyses revealed that the uptake of exogenous microRNAs by gastric pit cells is SIDT1 and low-pH dependent.Furthermore,oral administration of plant-derived miR2911 retards liver fibrosis,and this protective effect was abolished in SIDT1-deficient mice.Our findings reveal a major mechanism underlying the absorption of dietary microRNAs,uncover an unexpected role of the stomach and shed light on developing small RNA therapeutics by oral delivery. | Qun Chen Fan Zhang Lei Dong Huimin Wu Jie Xu Hanqin Li Jin Wang Zhen Zhou Chunyan Liu Yanbo Wang Yuyan Liu Liangsheng Lu Chen Wang Minghui Liu Xi Chen Cheng Wang Chunni Zhang Dangsheng Li Ke Zen Fangyu Wang Qipeng Zhang Chen-Yu Zhang | 2021 | Cell Research2021，31，3： | 10 |
| 15 | Generation of macaques with sperm derived from juvenile monkey testicular xenografts显示文摘 | Zhen Liu Yan-Hong Nie Chen-Chen Zhang Yi-Jun Cai Yan Wang Hui-Ping Lu Yu-zhuo Li Cheng Cheng Zi-Long Qiu Qiang Sun | 2016 | Cell Research2016，26，1： | 10 |
| 16 | MicroRNA-449a suppresses hepatocellular carcinoma cell growth via G1 phase arrest and the HGF/MET c-Met pathway显示文摘Background: Accumulating evidence demonstrates that microRNAs(miRNAs) play essential roles in tumorigenesis and cancer progression of hepatocellular carcinoma(HCC). Average targets of a miRNA were more than 100. And one miRNA may act in tumor via regulating several targets. The present study aimed to explore more potential targets of miR-449a by proteomics technology and further uncover the role of miR-449a in HCC tumorigenesis.Methods: Technologies such as i TRAQ-based quantitative proteomic were used to investigate the effect of miR-449a on HCC. The expression of c-Met and miR-449a was detected by q RT-PCR in HCC samples.Gain-and loss-of-function experiments were performed to identify the function and potential target of miR-449a in HCC cells.Results: In HCC, miR-449a was significantly downregulated, while c-Met was upregulated concurrently.Quantitative proteomics and luciferase reporter assay identified c-Met as a direct target of miR-449a.Moreover, miR-449a inhibited HCC growth not only by targeting CDK6 but also by suppressing cMet/Ras/Raf/ERK signaling pathway. Furthermore, the inhibition of c-Met expression with a specific siRNA significantly inhibited cells growth and deregulated the ERK pathway in HCC.Conclusion: The tumor suppressor miR-449a suppresses HCC tumorigenesis by repressing the c-Met/ERK pathway. | Jun Cheng Li-Ming Wu Xue-Song Deng Jian Wu Zhen Lv Hang-Fen Zhao Zhang Yang Yong Ni | 2018 | Hepatobiliary & Pancreatic Diseases International2018，17，4： | 10 |
| 17 | The ultratrace detection of crystal violet using surface enhanced Raman scattering on colloidal Ag nanoparticles prepared by electrolysis显示文摘高度活跃、稳定、买得起的表面提高了散布的拉曼(重量的单位)底层被 electrolyzing 获得 AgNO 3的混合物( 4 × ; 10 −4 mol/L )并且 Na 3 C 6 H 5 O 7·H2 O ( 6 × ; 10 −5 mol/L )为 1 ,在 7 V 的 2 , 3 和 4 h 。与水晶紫(CV ) 作为一个测试分子,有 785 nm 激光刺激的一个便携式的拉曼分光计被采用执行 SERS 察觉。胶体的 Ag nanoparticles 与粒子尺寸为 3 h 由 electrolyzing 准备了(65 ± 17 ) nm 是为 CV 的极端踪迹察觉的完美的重量的单位底层,它显示了 ca 的一个改进因素。1.3 × 10 8 和 CV 的察觉限制对 ca 感到不满。10 −15 mol/L (ca。10 −4 ppb ) 与是的 10 −1 mol/L KBr 聚集代理人。因此,这重量的单位底层将在极端踪迹察觉提供有希望的前景。同时,从实验室带给拉曼分析在 situ,即时察觉和鉴定处理将提供可能性。 | Ren Ming Liu Yi Pu Kang Xing Fa Zi Ming Jun Feng Man Cheng Min Zhen Si | 2009 | Chinese Chemical Letters2009，20，6： | 10 |
| 18 | A new multi-wavelength solar telescope: Optical and Near-infrared Solar Eruption Tracer (ONSET)显示文摘A new multi-wavelength solar telescope,the Optical and Near-infrared Solar Eruption Tracer(ONSET)of Nanjing University,has been constructed.It was fabricated at the Nanjing Institute of Astronomical Optics&Technology,and the operation is jointly administered with Yunnan Astronomical Observatory.ONSET is able to observe the Sun in three wavelength windows:He I 10830?A,Hαand white-light at3600?A and 4250?A,which are selected in order to simultaneously record the dynamics of the corona,chromosphere and photosphere respectively.Full-disk or partial-disk solar images with a field of 10 at three wavelengths can be obtained nearly simultaneously.It is designed to trace solar eruptions with high spatial and temporal resolutions.This telescope was installed at a new solar observing site near Fuxian Lake in Yunnan Province,southwest China.The site is located at E102N24,with an altitude of 1722 m.The seeing is stable and has high quality.We give a brief description of the scientific objectives and the basic structure of ONSET.Some preliminary results are also presented. | Cheng Fang Peng-Fei Chen Zhen Li Ming-De Ding Yu Dai Xiao-Yu Zhang Wei-Jun Mao Jun-Ping Zhang Ting Li Yong-Jun Liang Hai-Tian Lu | 2013 | Research in Astronomy and Astrophysics2013，13，12： | 9 |
| 19 | Flexible self-charging power units for portable electronics based on folded carbon paper显示文摘对便携式的电子学的迫切要求支持了高效率、持续、甚至可拉长的自我控告的力量来源的发展。在这个工作,我们建议为从人的运动和力量便携式计算机电子学收获机械精力基于合拢的碳(FC ) 统一纸的灵活自我控告的力量。现在的单位主要由摩擦电 nanogenerator (FC-TENG ) 和 supercapacitor (FC-SC ) 组成,基于合拢的复写纸精力 harvester 和存储设备分别地。这个有利几何图案向高幼仔模量复写纸提供优秀 stretchability 并且使力量单位能甚至在严重变丑下面工作,扭动,并且滚动,例如弯曲。另外,张力的紧张能被调节合拢三角的复写纸的合拢的角度最大化。而且,包装设备的防水性质使它可洗,保护它免受人的汗的伤害,并且使它能在严厉环境工作。最后,同样准备的自我控告的力量单位被把它放在人的身体上从手拍,踩的脚,并且手臂接触成功地驱动一块电子手表收获机械精力测试。这个工作表明可拉长的自我控告的力量单位的出色的潜力,它将进一步为便携 / 便携式的电子学支持高幼仔模量材料的发展。 | Changjie Zhou Yanqin Yang Na Sun Zhen Wen Ping Cheng Xinkai Xie Huiyun Shao Qingqing Shen Xiaoping Chen Yina Liu Zhong Lin Wang Xuhui Sun | 2018 | Nano Research2018，11，8： | 9 |
| 20 | Triboelectric–Electromagnetic Hybrid Generator for Harvesting Blue Energy显示文摘Progress has been developed in harvesting lowfrequency and irregular blue energy using a triboelectric–electromagnetic hybrid generator in recent years. However,the design of the high-efficiency, mechanically durable hybrid structure is still challenging. In this study, we report a fully packaged triboelectric–electromagnetic hybrid generator(TEHG), in which magnets were utilized as the trigger to drive contact–separation-mode triboelectric nanogenerators(CS-TENGs) and coupled with copper coils to operate rotary freestanding-mode electromagnetic generators(RF-EMGs). The magnet pairs that produce attraction were used to transfer the external mechanical energy to the CS-TENGs, and packaging of the CS-TENGpart was achieved to protect it from the ambient environment. Under a rotatory speed of 100 rpm, the CS-TENGs enabled the TEHG to deliver an output voltage, current,and average power of 315.8 V, 44.6 μA, and ~ 90.7 μW,and the output of the RF-EMGs was 0.59 V, 1.78 m A, and 79.6 μW, respectively. The cylinder-like structure made the TEHG more easily driven by water flow and demonstrated to work as a practical power source to charge commercial capacitors. It can charge a 33μF capacitor from 0 to 2.1 V in 84 s, and the stored energy in the capacitor can drive an electronic thermometer and form a self-powered water-temperature sensing system. | Huiyun Shao Ping Cheng Ruixuan Chen Lingjie Xie Na Sun Qingqing Shen Xiaoping Chen Qianqian Zhu Yi Zhang Yina Liu Zhen Wen Xuhui Sun | 2018 | Nano-Micro Letters2018，10，3： | 9 |
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