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| 1 | Generation of male germ cells from induced pluripotent stem cells (iPS cells): an in vitro and in vivo study显示文摘最近的研究报导了从老鼠和人的房间能区分进初发的细菌房间的那导致的 pluripotent 茎(iPS ) 。然而, iPS 房间是否能够生产男细菌房间,不被知道。这研究的目的进 spermatogonial 干细胞和迟了阶段的男细菌房间的老鼠 iPS 房间是调查区别潜在的。我们使用了在 vitro 区别并且在 vivo 移植联合的一条途径。胚胎植物或动物身体(EB ) 用白血球过多症禁止者因素(LIF ) 从 iPS 房间被获得免费媒介。量的 PCR 表明在 Oct4 表示的减少和 Stra8 的增加和在 EB 的 Vasa mRNA 源于 iPS 房间。iPS 导出房间的 EB 被区分进 spermatogonial 干细胞(SSC ) ,,的酸由他们 VASA,以及 CDH1 和 GFRα 的表示证实了的 retinoic 导致; 1,它是 SSC 的标记。而且,从 iPS 房间导出的这些细菌房间被移植进与 busulfan 被预先对待的老鼠的接受者睾丸。尤其是, iPS 导出房间的 SSC 能区分进从 spermatogonia 到圆 spermatids,由 VASA 和 SCP3 表示出现的男细菌房间。这研究证明 iPS 房间有潜力区分进迟了阶段的男细菌房间。从 iPS 房间的男细菌房间的推导在男不孕的处理有潜在的应用程序并且为揭开提供一个模型位于男细菌房间开发下面的分子的机制。 | Yong Zhu Hong-Liang Hu Peng Li Shi Yang Wei Zhang Hui Ding Ru-Hui Tian Ye Ning Ling-Ling Zhang Xi-Zhi Guo Zhan-Ping Shi Zheng Li Zuping He | 2012 | Asian Journal of Andrology2012，14，4： | 13 |
| 2 | Exosomes:key players in cancer and potential therapeutic strategy显示文摘Exosomes are extracellular vesicles secreted by most eukaryotic cells and participate in intercellular communication.The components of exosomes,including proteins,DNA,mRNA,microRNA,long noncoding RNA,circular RNA,etc.,which play a crucial role in regulating tumor growth,metastasis,and angiogenesis in the process of cancer development,and can be used as a prognostic marker and/or grading basis for tumor patients.Hereby,we mainly summarized as followed:the role of exosome contents in cancer,focusing on proteins and noncoding RNA;the interaction between exosomes and tumor microenvironment;the mechanisms that epithelial-mesenchymal transition,invasion and migration of tumor affected by exosomes;and tumor suppression strategies based on exosomes.Finally,the application potential of exosomes in clinical tumor diagnosis and therapy is prospected,which providing theoretical supports for using exosomes to serve precise tumor treatment in the clinic. | Jie Dai Yangzhou Su Suye Zhong Li Cong Bang Liu Junjun Yang Yongguang Tao Zuping He Chao Chen Yiqun Jiang | 2020 | Signal Transduction and Targeted Therapy2020，5，1： | 8 |
| 3 | Derivation of male germ cells from induced pluripotent stem (iPS) cells: a novel and crucial source for generating male gametes显示文摘 | Zuping He | 2012 | Asian Journal of Andrology2012，14，4： | 3 |
| 4 | NODAL secreted by male germ cells regulates the proliferation and function of human Sertoli cells from obstructive azoospermia and nonobstructive azoospermia patients显示文摘 | Ru-Hui Tian Shi Yang Zi-Jue Zhu Jun-Long Wang Yun Liu Chencheng Yao Meng Ma Ying Guo Qingqing Yuan Yanan Hai Yi-Ran Huang Zuping He Zheng Li | 2015 | Asian Journal of Andrology2015，17，6： | 2 |
| 5 | Advances in studies on hepatic stem cells显示文摘The question whether hepatic stem cells exist or not has been debated for several decades. Current researches confirm that there are hepatic stem cells in the liver. Oval cells, putative bipotential hepatic stem cells, are probably located within canals of Hering, portal tracts or branches of biliary trees. Bone marrow is a potential source of oval cells, indicating that there exists a close relationship between liver and hematopoiesis in adulthood. Hepatic stem cells are able to proliferate in vitro and can be induced to differentiate into hepatocytes. This will provide a promising approach of cell transplantation, tissue engineering and gene therapy for liver diseases. In this review, the evidence of their presence, origin, identification, proliferation in vitro, differentiation by induction, application prospects of hepatic stem cells and future directions for the field are discussed. | HE Zuping, TANG Yuefeng, LIU Yabing and FENG Meifu(State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China) | 2003 | Progress in Natural Science:Materials International2003，13，3： | 2 |
| 6 | Differentiation of Induced Pluripotent Stem Cells into Male Germ Cells In Vitro through Embryoid Body Formation and Retinoic Acid or Testosterone Induction显示文摘 | Peng Li Hongliang Hu Shi Yang Ruhui Tian Zhenzhen Zhang Wei Zhang Meng Ma Yong Zhu Xizhi Guo Yiran Huang Zuping He Zheng Li Xuan Jin | 2012 | BioMed Research International2012，，： | 2 |
| 7 | Plasticity of male germline stem cells and their applications in reproductive and regenerative medicine显示文摘Spermatogonial 干细胞(SSC ) ,也已知的同样男的 germline 干细胞,是小 subpopulation 与自强的潜力打 spermatogonia 在哺乳动物的睾丸维持干细胞水池和区别进 spermatids。自从他们能仅仅在生精的小管以内产生精子, SSC 以前被认为是 unipotent 干细胞。然而,因为众多的研究证明了与生长因素有教养的 SSC 能获得 pluripotency 成为胚胎的像茎的细胞,这个概念最近被质问了。在里面 vivo 并且在 vitro,从同伴和我们的研究清楚地表明了那 SSC 直接装 transdifferentiate 进词法,另外的系的 phenotypic,和功能的房间。到另外的纸巾的房间的直接变换为再生药有重要意义。从精子缺乏病人的 SSC 能被导致与授精和发展潜力区分进 spermatids。因此, SSC 能由于他们的唯一、大的潜力在繁殖、再生的药有重要应用。在这评论,我们探讨 SSC 的重要粘性,与他们的自强,区别, dedifferentiation, transdifferentiation,和翻译的药研究的焦点。 | Zheng Chen Zheng Li Zuping He | 2015 | Asian Journal of Andrology2015，17，3： | 2 |
| 8 | Cyclic di-adenosine monophosphate regulates the osteogenic and adipogenic differentiation of hPDLSCs via MAPK and NF-κB signaling显示文摘Cyclic di-adenosine monophosphate(c-di-AMP)is a bacterial second messenger that can be recognized by infected host cells and activate the immunoinflammatory response.The purpose of this study is to demonstrate the effect of c-di-AMP on the differentiation of human periodontal ligament stem cells(hPDLSCs)and its underlying mechanisms.In the present study,we find that the gingival crevicular fluid(GCF)of patients with chronic periodontitis has a higher expression level of c-di-AMP than that of healthy people.In vitro,c-di-AMP influences the differentiation of hPDLSCs by upregulating Toll-like receptors(TLRs);specifically,it inhibits osteogenic differentiation by activating NF-κB and ERK/MAPK and promotes adipogenic differentiation through the NF-κB and p38/MAPK signaling pathways.Inhibitors of TLRs or activated pathways reduce the changes induced by c-di-AMP.Our results establish the potential correlation among bacterial c-di-AMP,periodontal tissue homeostasis and chronic periodontitis pathogenesis. | Sirui Chen Zuping Wu Yuying He Li Zhu Jiahe Wang Hengyi Lin Jing Xie Chenchen Zhou Shujuan Zou | 2023 | Acta Biochimica et Biophysica Sinica2023，55，3： | 1 |
| 9 | Differentiation of Induced Pluripotent Stem Cells into Male Germ Cells In Vitro through Embryoid Body Formation and Retinoic Acid or Testosterone Induction显示文摘 | Peng Li Hongliang Hu Shi Yang Ruhui Tian Zhenzhen Zhang Wei Zhang Meng Ma Yong Zhu Xizhi Guo Yiran Huang Zuping He Zheng Li Xuan Jin | 2012 | BioMed Research International2012，，： | 1 |
| 10 | Gfralsilencing in mouse spermatogonial stem cells results in their differentiation via the inactivation of RET tyrosine kinase显示文摘 | HE Zuping JIANG Jiji HOFMANN M C | 2007 | Biol Reprod2007，77，4： | 1 |
| 11 | The roles and regulation of Yes-associated protein 1 in stem cells显示文摘Yes-associated protein 1(YAP1)is a downstream effector of the Hippo signaling pathway,and it is involved in tumorigenesis,tissue repair,growth,and development.In this review,the biological roles and the mechanisms of YAP1 in mediating stem cell fate decisions are discussed,including cell proliferation,differentiation,and apoptosis.In general,YAP1 promotes the proliferation and differentiation of stem cells,including embryonic stem cells and adult stem cells.It inhibits apoptosis by binding to the transcription factors,e.g.,transcriptional enhanced associate domain(TEAD),Smad,runt-related transcription factor 1/2,p73,p63,and Erb84,to maintain tissue homeostasis.The translocalization of YAP1 in cellular nuclei and the phosphorylation in the cytoplasm work as important and unusual events for the activation of YAP1.Moreover,YAP1 serves as the crosstalk for the Hippo pathway and other signaling pathways,including the Wnt and Notch pathways.It is highlighted in this review that YAP1 is an essential regulator for stem cells that have significant applications in regenerative medicine and reproductive medicine. | QING YIN CAIHONG LIU WENYING JIANG HAIHUI GONG CHUNYUN LI ZUPING HE | 2023 | BIOCELL2023，47，1： | 0 |
| 12 | Generation of male germ cells in vitro from the stem cells显示文摘Infertility has become a serious disease since it affects 10%–15%of couples worldwide,and male infertility contributes to about 50%of the cases.Notably,a significant decrease occurs in the newborn population by 7.82 million in 2020 compared to 2016 in China.As such,it is essential to explore the effective methods of obtaining functional male gametes for restoring male fertility.Stem cells,including embryonic stem cells(ESCs),induced pluripotent stem cells(iPSCs),spermatogonial stem cells(SSCs),and mesenchymal stem cells(MSCs),possess the abilities of both self-renewal and differentiation into germ cells.Significantly,much progress has recently been achieved in the generation of male germ cells in vitro from various kinds of stem cells under the specified conditions,e.g.,the coculturing with Sertoli cells,three-dimensional culture system,the addition of growth factors and cytokines,and/or the overexpression of germ cell-related genes.In this review,we address the current advance in the derivation of male germ cells in vitro from stem cells based on the studies of the peers and us,and we highlight the perspectives and potential application of stem cell-derived male gametes in reproductive medicine. | Ying-Hong Cui Wei Chen Si Wu Cai-Lin Wan Zuping He | 2023 | Asian Journal of Andrology2023，25，1： | 0 |
| 13 | Generation of functional organs from stem cells显示文摘We are now well entering the exciting era of stem cells.Potential stem cell therapy holds great promise for the treatment of many diseases such as stroke,traumatic brain injury,Alzheimer’s disease,Parkinson’s disease,amyotrophic lateral-sclerosis,myocardial infarction,muscular dystrophy,diabetes,and etc..It is generally believed that transplantation of specific stem cells into the injured tissue to replace the lost cells is an effective way to repair the tissue.In fact,organ transplantation has been successfully practiced in clinics for liver or kidney failure.However,the severe shortage of donor organs has been a major obstacle for the expansion of organ transplantation programs.Toward that direction,generation of transplantable organs using stem cells is a desirable approach for organ replacement and would be of great interest for both basic and clinical scientists.Here we review recent progress in the field of organ generation using various methods including single adult tissue stem cells,a blastocyst complementation system,tissue decellularization/recellularization and a combination of stem cells and tissue engineering. | Yunying Liu Ru Yang Zuping He Wei-Qiang Gao | 2013 | Cell Regeneration2013，2，1： | 0 |
| 14 | The role of homozygous LOF variant of the PNLDC1 gene in oligo-astheno-teratozoospermia(OAT)and male infertility显示文摘Dear Editor,An interesting article from Zhao et al.recently published in Asian Journal of Andrology,has shown the association of a novel loss-of function(LOF)variant in PARN-like ribonuclease domain-containing exonuclease 1(PNLDCI)and male infertility,and we would like to contribute a commentary on this article. | Zi-Xin Cheng Li Du Zuping He | 2023 | Asian Journal of Andrology2023，25，6： | 0 |
| 15 | Characterization, isolation, and culture of spermatogonial stem cells in Macaca fascicularis显示文摘Spermatogonial stem cells(SSCs)have great applications in both reproductive and regenerative medicine.Primates including monkeys are very similar to humans with regard to physiology and pathology.Nevertheless,little is known about the isolation,the characteristics,and the culture of primate SSCs.This study was designed to identify,isolate,and culture monkey SSCs.Immunocytochemistry was used to identify markers for monkey SSCs.Glial cell line-derived neurotrophic factor family receptor alpha-1(GFRAl)-enriched spermatogonia were isolated from monkeys,namely Macaca fascicularis(M.fascicularis),by two-step enzymatic digestion and magnetic-activated cell sorting,and they were cultured on precoated plates in the conditioned medium.Reverse transcription-polymerase chain reaction(RT-PCR),immunocytochemistry,and RNA sequencing were used to compare phenotype and transcriptomes in GFRAl-enriched spermatogonia between 0 day and 14 days of culture,and xenotransplantation was performed to evaluate the function of GFRAl-enriched spermatogonia.SSCs shared some phenotypes with rodent and human SSCs.GFRAl-enriched spermatogonia with high purity and viability were isolated from M.fascicularis testes.The freshly isolated cells expressed numerous markers for rodent SSCs,and they were cultured for 14 days.The expression of numerous SSC markers was maintained during the cultivation of GFRAl-enriched spermatogonia.RNA sequencing reflected a 97.3%similarity in global gene profiles between 0 day and 14 days of culture.The xenotransplantation assay indicated that the GFRAl-enriched spermatogonia formed colonies and proliferated in vivo in the recipient c-Kitw/w(W)mutant mice.Collectively,GFRAl-enriched spermatogonia are monkey SSCs phenotypically both in vitro and in vivo.This study suggests that monkey might provide an alternative to human SSCs for basic research and application in human diseases. | Guo-Ping Mao Ming-Hui Niu Ying-Hong Cui Rui-Ling Tang Wei Chen Bang Liu Zuping He | 2021 | Asian Journal of Andrology2021，23，3： | 0 |
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