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| 1 | Development of cell-based pseudovirus entry assay to identify potential viral entry inhibitors and neutralizing antibodies against SARS-CoV-2显示文摘Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)is the causative virus of the coronavirus disease 2019(COVID-19)pandemic.To establish a safe and convenient assay system for studying entry inhibitors and neutralizing antibodies against SARS-CoV-2,we constructed a codon-optimized,full-length C-terminal mutant spike(S)gene of SARS-CoV-2.We generated a luciferase(Luc)-expressing pseudovirus containing the wild-type or mutant S protein of SARS-CoV-2 in the envelope-defective HIV-1 backbone.The key parameters for this pseudovirus-based assay,including the S mutants and virus incubation time,were optimized.This pseudovirus contains a Luc reporter gene that enabled us to easily quantify virus entry into angiotensin-converting enzyme 2(ACE2)-expressing 293T cells.Cathepsin(Cat)B/L inhibitor E64d could significantly block SARS-CoV-2 pseudovirus infection in 293T-ACE2 cells.Furthermore,the SARS-CoV-2 spike pseudotyped virus could be neutralized by sera from convalescent COVID-19 patients or recombinant ACE2 with the fused Fc region of human IgG1.Thus,we developed a pseudovirus-based assay for SARS-CoV-2,which will be valuable for evaluating viral entry inhibitors and neutralizing antibodies against this highly pathogenic virus. | Jie Hu Qingzhu Gao Changlong He Ailong Huang Ni Tang Kai Wang | 2020 | Genes & Diseases2020，7，4： | 15 |
| 2 | Electrochemical Intramolecular C-H/O-H Cross-Coupling of 2-Arylbenzoic Acidst显示文摘 | Ailong Shao Na Li Yong Gao Jirui Zhan Chien-Wei Chiang Aiwen Lei | 2018 | Chinese Journal of Chemistry2018，36，7： | 7 |
| 3 | Patients with SARS-CoV-2 and HBV coinfection are at risk of greater liver injury显示文摘To date,it remains unclear if severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)co-infection exacerbates liver injury in patients with chronic hepatitis B virus(HBV)infection.In this study,we present a retrospective study of 133 hospitalized confirmed mild coronavirus disease 2019(COVID-19)cases,including 116 patients with COVID-19 with negative serum hepatitis B antigen and 17 HBV inactive carriers with COVID-19.We found that there were no significant differences for the discharge rate or duration of hospitalization be-tween the two groups.However,inactive HBV carriers with SARS-CoV-2 co-infection are at a higher risk of abnormal liver function tests.The enhanced liver injury induced by SARS-CoV-2 and HBV co-infection was identified as the hepatocyte type rather than the cholangiocyte type.Moreover,the inflammatory response,including abnormal lactate dehydrogenase,D-dimer and interleukin-6 production,may contribute to this injury following SARS-CoV-2 co-infection.Collectively,SARS-CoV-2 and HBV co-infection exacerbates liver function of the pa-tients with COVID-19. | Yong Lin Jun Yuan Quanxin Long Jieli Hu Haijun Deng Zhenyu Zhao Juan Chen Mengji Lu Ailong Huang | 2021 | Genes & Diseases2021，8，4： | 5 |
| 4 | A multi-epitope vaccine based on Chlamydia trachomatis major outer membrane protein induces specific immunity in mice显示文摘我们评估了包括主要的外部膜蛋白质(MOMP ) 的一支候选人疫苗的 immunogenicity 和功效 multi-epitopeof Chlamydia trachomatis。从包含多重 T 房间和 B 房间 epitopes 的 MOMP 导出的 multi-epitope 的短基因是人工地,包含 codon 的 synthesized.The recombinant plasmid pET32a (+) 优化了 MOMP multi-epitope 基因被构造。在 Escherichia coli 的 fusionprotein Trx-His-MOMP multi-epitope 的表示被钠 dodecyl sulfatepolyacrylamide 胶化电气泳动和西方的污点分析证实。Balb/c 老鼠是 inoculatedwith 皮下地,三与 2 星期的间隔预定的净化的熔化蛋白质。结果出现了 MOMP multi-epitopeelicited 不仅对 C 的强壮的体液的有免疫力的回答。由显著地产生特定的抗体(IgG1 和 IgG2a ) 的高水平的 trachomatis,而且由 inducingrobust 的细胞的有免疫力的回答在老鼠的细胞毒素的 T 淋巴细胞回答。而且, MOMP multi-epitope 实质地告知 IFN- 的分泌物,表明这支疫苗能导致强壮的 Th1 回答。最后,老鼠与 C 的 MOMP multi-epitope displayeda 减小种牛痘。在 chlamydial 挑战和感染的 C 的一个加速的清理之上流的 trachomatis。trachomatis。在结论, MOMP multi-epitope 疫苗可以对 C 为有效预防 andtherapeutic 疫苗的发展有潜力。trachomatis 感染。 | Jianxin Tu B ailong Hou Bingbing Wang Xiaoyun Lin Wenci Gong Haiyan Dong Shanli Zhu Shao Chen Xiangyang Xue Kong-Nan Zhao Lifang Zhang | 2014 | Acta Biochimica et Biophysica Sinica2014，46，5： | 1 |
| 5 | MicroRNAs‐372/373 promote the expression of hepatitis B virus through the targeting of nuclear factor I/B显示文摘 | Hongyan Guo Haiying Liu Keith Mitchelson Huiying Rao Mingyong Luo Lan Xie Yimin Sun Liang Zhang Ying Lu Ruyu Liu Aihui Ren Shuai Liu Shaozhen Zhou Jiye Zhu Yuxiang Zhou Ailong Huang Lai Wei Yong Guo Jing Cheng | 2011 | Hepatology2011，，3： | 1 |
| 6 | Towards robust immune responses after heterologous COVID-19 vaccination and its application perspectives显示文摘The coronavirus disease 2019(COVID-19)pandemic,caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)[1],has seriously impacted the global health and economy.Effective vaccination,with homologous or heterologous prime-boost strategies,is the key to controlling the ongoing COVID-19 pandemic[2].Homologous vaccinations,which are the administration of the same type of COVID-19 vaccine,have been shown to be highly efficient in inducing robust immune responses[3–7]. | Yong Lin Ailong Huang | 2022 | National Science Open2022，1，1： | 1 |
| 7 | Hepatitis B virus regulation of Raf1 promoter activity through activation of transcription factor AP-2α显示文摘 | Jialin Qu Jianbo Li Ke Chen Dongdong Qin Kai Li Yanrui Sheng Chengcheng Zou Sen Wang Ailong Huang Hua Tang | 2013 | Archives of Virology2013，，4： | 1 |
| 8 | Design, Synthesis and Biological Evaluation of Sulfenimine Cephalosporin Analogues as β-Lactamase Inhibitors显示文摘 | ZHANG Kai DING Huaiwei SHI Ailong HUANG Qi SONG Hongrui FU Decai | 2015 | Chemical Research in Chinese Universities2015，31，3： | 1 |
| 9 | Effect of hot humid environmental exposure on fatigue crack growth of adhesive-bonded aluminum A356 joints 显示文摘 | Wang Mingxing Liu Ailong Liu Zhongxia ct al | 2013 | International Journal of Adhesion & Adhesives2013，40，： | 1 |
| 10 | Correlation between hepatitis B virus protein and microRNA processor Drosha in cells expressing HBV显示文摘 | Min Ren Dongdong Qin Kai Li Jialin Qu Liying Wang Zengchan Wang Ailong Huang Hua Tang | 2012 | Antiviral Research2012，，3： | 1 |
| 11 | Characterization of SARS-CoV-2-specific humoral immunity and its potential applications and therapeutic prospects显示文摘Coronavirus disease 2019(COVID-19),caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),is an ongoing pandemic that poses a great threat to human health worldwide.As the humoral immune response plays essential roles in disease occurrence and development,understanding the dynamics and characteristics of virus-specific humoral immunity in SARS-CoV-2-infected patients is of great importance for controlling this disease.In this review,we summarize the characteristics of the humoral immune response after SARS-CoV-2 infection and further emphasize the potential applications and therapeutic prospects of SARSCoV-2-specific humoral immunity and the critical role of this immunity in vaccine development.Notably,serological antibody testing based on the humoral immune response can guide public health measures and control strategies;however,it is not recommended for population surveys in areas with very low prevalence.Existing evidence suggests that asymptomatic individuals have a weaker immune response to SARS-CoV-2 infection,whereas SARS-CoV-2-infected children have a more effective humoral immune response than adults.The correlations between antibody(especially neutralizing antibody)titers and protection against SARS-CoV-2 reinfection should be further examined.In addition,the emergence of cross-reactions among different coronavirus antigens in the development of screening technology and the risk of antibody-dependent enhancement related to SARS-CoV-2 vaccination should be given further attention. | Jiaxin Zheng Yingying Deng Zhenyu Zhao Binli Mao Mengji Lu Yong Lin Ailong Huang | 2022 | Cellular & Molecular Immunology2022，19，2： | 1 |
| 12 | Anti-synchronization control of a class of memristive recurrent neural networks显示文摘 | Wu Ailong Zeng Zhigang | 2013 | Communications in Nonlinear Science and Numerical Simulation2013，18，2： | 1 |
| 13 | Enhanced 2,3-butanediol production by Klebsiella pneumoniae SDM显示文摘 | Cuiqing Ma Ailong Wang Jiayang Qin Lixiang Li Xulu Ai Tianyi Jiang Hongzhi Tang Ping Xu | 2009 | Applied Microbiology and Biotechnology2009，，1： | 1 |
| 14 | Coarse-to-fine waterlogging probability assessment based on remote sensing image and social media data显示文摘Urban waterlogging probability assessment is critical to emergency response and policymaking.Remote Sensing(RS)is a rich and reliable data source for waterlogging monitoring and evaluation through water body extraction derived from the pre-and post-disaster RS images.However,RS images are usually limited to the revisit cycle and cloud cover.To solve this issue,social media data have been considered as another data source which are immune to the weather such as clouds and can reflect the real-time public response for disaster,which leads itself a compensation for RS images.In this paper,we propose a coarse-to-fine waterlogging probability assessment framework based on multisource data including real-time social media data,near real-time RS image and historical geographic information,in which a coarse waterlogging probability map is refined by using the real-time information extracted from social media data to acquire a more accurate waterlogging probability.Firstly,to generate a coarse waterlogging probability map,the historical inundated areas are derived from Digital Elevation Model(DEM)and historical waterlogging points,then the geographic features are extracted from DEM and RS image,which will be input to a Random Forest(RF)classifier to estimate the likelihood of hazards.Secondly,the real-time waterlogging-related information is extracted from social media data,where the Convolutional Neural Network(CNN)model is applied to exploit the semantic information of sentences by capturing the local and position-invariant features using convolution kernel.Finally,fine waterlogging probability map scan be generated based on morphological method,in which real-time waterlogging-related social media data are taken as isolated highlight point and used to refine the coarse waterlogging probability map by a gray dilation pattern considering the distance-decay effect.The 2016 Wuhan waterlogging and 2018 Chengdu water-logging are taken as case studies to demonstrate the effectiveness of the proposed framework.It can be concluded from the results that by integrating RS image and social media data,more accurate waterlogging probability maps can be generated,which can be further applied for inundated areas identification and disaster monitoring. | Lei Xu Ailong Ma | 2021 | Geo-Spatial Information Science2021，24，2： | 1 |
| 15 | Heparin sulphate d -glucosaminyl 3-O-sulfotransferase 3B1 plays a role in HBV replication显示文摘 | Zhenzhen Zhang Xiang Liu Juan Chen Huaibin Su Qiang Luo Jia Ye Ni Tang Wenlu Zhang WeiXian Chen Ben C.B. Ko Ailong Huang | 2010 | Virology2010，，2： | 1 |
| 16 | A potent neutralizing antibody provides protection against SARS-CoV-2 Omicron and Delta variants via nasal delivery显示文摘Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)is still rapidly spreading worldwide.Many drugs and vaccines have been approved for clinical use show efficacy in the treatment and prevention of SARS-CoV-2 infections.However,the emergence of SARS-CoV-2 variants of concern(VOCs),such as Delta(B.1.617.2)and the recently emerged Omicron(B.1.1.529),has seriously challenged the application of current therapeutics.Therefore,there is still a pressing need for identification of new broad-spectrum antivirals.Here,we further characterized a human antibody(58G6),which we previously isolated from a patient,with a broadly authentic virus-neutralizing activity that inhibits the Delta and Omicron variants with half-maximal inhibitory concentrations(ICso)of 1.69 ng/ml and 54.31 ng/ml,respectively.58G6 shows prophylactic and therapeutic effcacy in hamsters challenged with the Delta and Omicron variants through nasal delivery.Notably,a very low dosage(2 mg/kg daily)of 58G6 efficiently prevented Omicron variant replication in the lungs.These advantages may overcome the efficacy limitation of currently approved neutralizing antibodies that can be administered only by intravenous injection.In general,58G6 is a promising prophylactic and therapeutic candidate against current circulating VOCs and even future emerging mutants.To the best of our knowledge,58G6 is one of the most potent neutralizing antibodies against Omicron,with a broader spectrum than those approved for clinical use.58G6 could be developed as a nebulized therapy,which would be more cost effective and user friendly and enhance the clinical outcome comparedto thatobtainedwithdirect nasaldelivery. | Xinghai Zhang Huajun Zhang Tingting Li Shaohong Chen Feiyang Luo Junhui Zhou Peiyi Zheng Shuyi Song Yan Wu Tengchuan Jin Ni Tang Aishun Jin Chengyong Yang Guofeng Cheng Rui Gong Sandra Chiu Ailong Huang | 2022 | Signal Transduction and Targeted Therapy2022，7，9： | 1 |
| 17 | Exponential Passivity of Memristive Neural Netw orks with Time Delays显示文摘 | Wu Ailong Zeng Zhigang | 2014 | Neural Netw orks2014，49，1： | 1 |
| 18 | Opinion on the Policy of Lifting Restrictions to Entry Under the Circumstance of the COVID-19 Pandemic显示文摘One of the serious consequences of the coronavirus disease 2019(COVID-19)pandemic is the extreme restriction on international contacts.Communication between some countries has almost been frozen for a period of time,which is a manifestation of“social distancing”,a strategy for epidemic prevention and control,among countries.In the context of economic globalization,“social distancing”has caused serious adverse effects,including poor communication in society,the economy and personnel,reduced efficiency of resource allocation,and increased costs.Therefore,people should take necessary measures to restore international contact and eliminate these adverse effects as soon as possible. | Ailong Huang Jieli Hu Hong Zhang | 2021 | Infectious Diseases & Immunity2021，1，3： | 0 |
| 19 | Characterization of Specific Humoral Immunity in Asymptomatic SARS-CoV-2 Infection显示文摘The outbreak of the severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)infection in December 2019 caused a huge blow to both global public health and global economy.At the early stage of the coronavirus disease 2019(COVID-19)epidemic,asymptomatic individuals with SARS-CoV-2 infection were ignored,without appropriate identification and isolation.However,asymptomatic individuals proved to comprise a high proportion of all SARS-CoV-2-infected individuals,which greatly contributed to the rapid and wide spread of this disease.In this review,we summarize the latest advances in epidemiological characteristics,diagnostic assessment methods,factors related to the establishment of SARS-CoV-2 asymptomatic infection,as well as humoral immune features after SARS-CoV-2 infection or vaccination in asymptomatic individuals,which would contribute to effective control of ongoing COVID-19 epidemic. | Yingying Deng Jiaxin Zheng Yong Lin Ailong Huang | 2021 | Infectious Diseases & Immunity2021，1，3： | 0 |
| 20 | Real-world effectiveness of an intranasal spray A8G6 antibody cocktail in the post-exposure prophylaxis of COVID-19显示文摘Previously,we identified an antibody combination A8G6 that showed promising efficacy in COVID-19 animal models and favorable safety profile in preclinical models as well as in a first-in-human trial.To evaluate the real-word efficacy of A8G6 neutralizing antibody nasal spray in post-exposure prophylaxis of COVID-19,an open-label,non-randomized,two-arm,blank-controlled,investigator-initiated trial was conducted in Chongqing,China(the register number:ChiCTR2200066416).High-risk healthy participants(18–65 years)within 72 h after close contact to COVID-19 patients were recruited and received a three-dose(1.4 mg/dose)A8G6 treatment daily or no treatment(blank control)for 7 consecutive days.SARS-CoV-2 infection occurred in 151/340(44.4%)subjects in the blank control group and 12/173(6.9%)subjects in the A8G6 treatment group.The prevention efficacy of the A8G6 treatment within 72 h exposure was calculated to be 84.4%(95%CI:74.4–90.4%).Moreover,compared to the blank-control group,the time from the SARS-CoV-2 negative to the positive COVID-19 conversion was significantly longer in the AG86 treatment group(mean time:3.4 days vs 2.6 days,p=0.019).In the secondary end-point analysis,the A8G6 nasal treatment had no effects on the viral load at baseline SARS-CoV-2 RT-PCR positivity and the time of the negative COVID-19 conversion.Finally,except for 5 participants(3.1%)with general adverse effects,we did not observe any severe adverse effects related to the A8G6 treatment.In this study,the intranasal spray AG86 antibody cocktail showed potent efficacy for prevention of SARS-CoV-2 infection in close contacts of COVID-19 patients. | Xiaosong Li Pai Peng Haijun Deng Qian Yang Shi Chen Benhua Li Miao He Aishun Jin Zhu Yang Ni Tang Ailong Huang | 2023 | Signal Transduction and Targeted Therapy2023，8，11： | 0 |
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