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| 1 | Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system显示文摘允许的技术内长的基因的特定的规定为基因功能的学习是珍贵的并且在治疗学有大潜力。我们创造了在 CRISPR 上系统,由核酸酶死者 Cas9 (dCas9 ) 蛋白质组成的二部件的 transcriptional 使活跃之物与互补顺序与 transcriptional 激活域和单个指南 RNA (sgRNAs ) 熔化了到基因倡导者。我们证明在 CRISPR 上能高效地以一种悦耳的方式在人和老鼠房间激活外长的记者基因。另外,我们证明在 vivo 的柔韧的记者基因激活能被把系统部件注入老鼠接合子完成。而且,我们证明在 CRISPR 上能激活内长的 IL1RN, SOX2,和 OCT4 基因。最有效的基因激活被对近似倡导者有约束力的 3-4 sgRNAs 的簇完成,建议他们在基因正式就职的 synergistic 行动。显著地,当指向多重基因的 sgRNAs 同时被介绍进房间时,柔韧的多路的内长的基因激活被完成。染色体宽的表示介绍表明了系统的高特性。 | Albert W Cheng Haoyi Wang Hui Yang Linyu Shi Yarden Katz Thorold W Theunissen Sudharshan Rangarajan Chikdu S Shivalila Daniel B Dadon Rudolf Jaenisch | 2013 | Cell Research2013，23，10： | 65 |
| 2 | CRISPR-Cas9-mediated multiplex gene editing in CAR-T cells显示文摘 | Xiaojuan Liu Yongping Zhang Chen Cheng Albert W Cheng Xingying Zhang Na Li Changqing Xia Xiaofei Wei Xiang Liu Haoyi Wang | 2017 | Cell Research2017，27，1： | 40 |
| 3 | High-intensity focused ultrasound ablation:An effective bridging therapy for hepatocellular carcinoma patients显示文摘AIM:To analyze whether high-intensity focused ultrasound(HIFU) ablation is an effective bridging therapy for patients with hepatocellular carcinoma(HCC).METHODS:From January 2007 to December 2010,49 consecutive HCC patients were listed for liver transplantation(UCSF criteria).The median waiting time for transplantation was 9.5 mo.Twenty-nine patients received transarterial chemoembolization(TACE) as a bringing therapy and 16 patients received no treatment before transplantation.Five patients received HIFU ablation as a bridging therapy.Another five patients with the same tumor staging(within the UCSF criteria) who received HIFU ablation but not on the transplant list were included for comparison.Patients were comparable in terms of Child-Pugh and model for end-stage liver disease scores,tumor size and number,and cause of cirrhosis.RESULTS:The HIFU group and TACE group showed no difference in terms of tumor size and tumor number.One patient in the HIFU group and no patient in the TACE group had gross ascites.The median hospital stay was 1 d(range,1-21 d) in the TACE group and two days(range,1-9 d) in the HIFU group(P < 0.000).No HIFU-related complication occurred.In the HIFU group,nine patients(90%) had complete response and one patient(10%) had partial response to the treatment.In the TACE group,only one patient(3%) had response to the treatment while 14 patients(48%) had stable disease and 14 patients(48%) had progressive disease(P = 0.00).Seven patients in the TACE group and no patient in the HIFU group dropped out from the transplant waiting list(P = 0.559).CONCLUSION:HIFU ablation is safe and effective in the treatment of HCC for patients with advanced cirrhosis.It may reduce the drop-out rate of liver transplant candidate. | Tan To Cheung Sheung Tat Fan See Ching Chan Kenneth SH Chok Ferdinand SK Chu Caroline R Jenkins Regina CL Lo James YY Fung Albert CY Chan William W Sharr Simon HY Tsang Wing Chiu Dai Ronnie TP Poon Chung Mau Lo | 2013 | World Journal of Gastroenterology2013，19，20： | 23 |
| 4 | Casilio: a versatile CRISPR-Cas9-Pumilio hybrid for gene regulation and genomic labeling显示文摘 | Albert W Cheng Nathaniel Jillette Phoebe Lee Dylan Plaskon Yasuhiro Fuj iwara Wenbo Wang Aziz Taghbalout Haoyi Wang | 2016 | Cell Research2016，26，2： | 19 |
| 5 | Modulation of graft vascular inflow guided by flowmetry and manometry in liver transplantation显示文摘BACKGROUND:Survival of the partial graft after living donor liver transplantation owes much to its tremendous regenerative ability.With excellent venous outflow capacity,a graft within a wide range of graft-to-standard-liver-volume ratios can cope with portal hypertension that is common in liver transplant recipients.However,when the ratio range is exceeded,modulation of graft vascular inflow becomes necessary for graft survival.The interplay between graft-to-standard-liver-volume ratio and portal pressure,in the presence of portosystemic shunt or otherwise,requires individualized modulation of graft portal and arterial inflows.Boosting of portal inflow by shunt ligation can be guided by transonic flowmetry,whereas muting of portal inflow by splenic artery ligation can be monitored by portal electronic manometry.METHOD:We describe four cases to illustrate the above.RESULTS:One patient had hepatic artery thrombosis resulting from splenic artery steal syndrome which was the sequela of small-for-size syndrome.Emergency splenic artery ligation and re-anastomosis of the hepatic artery successfully muted the portal inflow and boosted the hepatic arterial inflow.Another patient with portal vein thrombosis underwent thrombendvenectomy.Portal inflow was boosted with ligation of portosystemic shunt,which is often present in these patients with portal hypertension.The coexistence of splenic aneurysm and splenorenal shunt required ligation of both in the third patient.The fourth patient,with portal pressure and flow monitoring,avoided ligation of a coronary vein which became a main portal inflow after portal thrombendvenectomy.CONCLUSION:Management of graft inflow modulation guided selectively by transonic flowmetry or portal manometry was described. | See Ching Chan Chung Mau Lo Kenneth SH Chok William W Sharr Tan To Cheung Simon HY Tsang Albert CY Chan Sheung Tat Fan | 2011 | Hepatobiliary & Pancreatic Diseases International2011，10，6： | 5 |
| 6 | General competencies of problem‐based learning (PBL) and non‐PBL graduates显示文摘 | Katinka J A HPrince Patrick W L JVan Eijs Henny P ABoshuizen Cees P MVan Der Vleuten Albert J J AScherpbier | 2005 | Medical Education2005，，4： | 5 |
| 7 | 早产儿视网膜病变研究近况显示文摘早产儿视网膜病变的主要表现为其初生视网膜毛细血管网发育异常,重者可引起视网膜脱离而导致失明。本文对早产儿视网膜病变的历史、流行病学、危险因素、分类方法、检查方法、预防、治疗和预后进行了总结性的全面介绍。其中早产儿视网膜病变国际分类法简明实用,并有图解说明,一目了然。治疗主要是冷冻,简便易行,而且对防止后部视网膜脱离、视网膜皱襞及晶体后纤维组织增生等有效。对于视力减退、斜视、近视、角膜病变、青光眼和视网膜并发症等有关问题也进行了较详细的分析。作者认为要减少出生前后发生早产几视网膜病变,必须做到以下数点:(1) 澄清各种危险因素;(2) 查出累及正常和异常视网膜血管形成的血管原性物质;(3) 降低早产的发生率;(4) 弄清诸如维生素E之类的预防性医疗韵价值。 | Albert W Biglan 刘轶凡 | 1990 | 国外医学(眼科学分册)1990，14，1： | 4 |
| 8 | Deceleration capacity of heart rate as a predictor of mortality after myocardial infarction: cohort study显示文摘 | Axel Bauer Jan W Kantelhardt Petra Barthel Raphael Schneider Timo M?kikallio Kurt Ulm Katerina Hnatkova Albert Sch?mig Heikki Huikuri Armin Bunde Marek Malik Georg Schmidt | 2006 | The Lancet2006，，9523： | 3 |
| 9 | Pyruvate-fortified resuscitation stabilizes cardiac electrical activity and energy metabolism during hypovolemia显示文摘AIM: To test the hypothesis that fluid resuscitation with Ringer's solution enriched with pyruvate(PR), a physiological antioxidant and energy substrate, affords protection of myocardial metabolism and electrophysiological performance superior to lactated Ringer's(LR) during hypovolemia and hindlimb ischemia-reperfusion.METHODS: Male domestic goats(25-30 kg) were exsanguinated to a mean arterial pressure of 48 ± 1 mm Hg. Right hindlimb ischemia was imposed for 90 min by applying a tourniquet and femoral crossclamp. LR or PR, infused iv, delivered 0.05 mmol/kg per minute L-lactate or pyruvate, respectively, from 30 min hindlimb ischemia until 30 min post-ischemia. Time controls(TC) underwent neither hemorrhage, hindlimb ischemia nor resuscitation. Goats were sacrificed and left ventricular myocardium biopsied at 90 min fluid resuscitation(n = 6 per group) or 3.5 h later(n = 9 LR, 10 PR, 8 TC).RESULTS: Myocardial 8-isoprostane content, phosphocreatine phosphorylation potential, creatine kinase activity, and heart rate-adjusted QT interval(QTc) vari- ability were evaluated at 90 min resuscitation and 3.5 h post-resuscitation. PR sharply lowered pro-arrhythmic QTc variability vs LR(P < 0.05); this effect persisted 3.5 h post-resuscitation. PR lowered myocardial 8-isoprostane content, a product of oxidative stress, by 39 and 37% during and 3.5 h after resuscitation, respectively, vs LR. Creatine kinase activity fell 42% post-LR vs TC(P < 0.05), but was stable post-PR(P < 0.02 vs post-LR). PR doubled phosphocreatine phosphorylation potential, a measure of ATP free energy state, vs TC and LR(P < 0.05); this energetic enhancement persisted 3.5 h post-resuscitation.CONCLUSION: By augmenting myocardial energy state and protecting creatine kinase activity, pyruvateenriched resuscitation stabilized cardiac electrical function during central hypovolemia and hindlimb ischemiareperfusion. | Hunaid A Gurji Daniel W White Besim Hoxha Jie Sun Albert H Olivencia-Yurvati Robert T Mallet | 2013 | World Journal of Critical Care Medicine2013，2，4： | 3 |
| 10 | The Baffled Flask Test for Dispersant Effectiveness: A Round Robin Evaluation of Reproducibility and Repeatability显示文摘 | Albert D Venosa Dennis W King George A Sorial | 2002 | Spill Science and Technology Bulletin2002，，5： | 2 |
| 11 | Kontrastmittelsonografie bei B-Bild-morphologisch unklaren Leberraumforderungen – Diagnostische Treffsicherheit im klinischen Alltag (DEGUM-Multicenter – Studie)显示文摘 | D Strobel K Seitz W Blank A Schuler C Dietrich A Herbay M Friedrich-Rust G Kunze D Becker U Will W Kratzer F Albert C Pachmann K Dirks H Strunk C Greis T Bernatik | 2008 | Ultraschall in Med2008，，05： | 2 |
| 12 | Retrohepatic vena cava deroofing in living donor liver transplantation for caudate hepatocellular carcinoma显示文摘The removal of tumor together with the native liver in living donor liver transplantation for hepatocellular carcinoma is challenged by a very close resection margin if the tumor abuts the inferior vena cava.This is in contrast to typical deceased donor liver transplantation where the entire retrohepatic inferior vena cava is included in total hepatectomy.Here we report a case of deroofing the retrohepatic vena cava in living donor liver transplantation for caudate hepatocellular carcinoma.In order to ensure clear resection margins,the anterior portion of the inferior vena cava was included.The right liver graft was inset into a Dacron vascular graft on the back table and the composite graft was then implanted to the recipient inferior vena cava.Using this technique,we observed the no-touch technique in tumor removal,hence minimizing the chance of positive resection margin as well as the chance of shedding of tumor cells during manipulation in operation. | See Ching Chan William W Sharr Tan To Cheung Albert CY Chan Simon HY Tsang Kenneth SH Chok Kin Chung Leung Chung Mau Lo | 2013 | Hepatobiliary & Pancreatic Diseases International2013，12，5： | 2 |
| 13 | Sediment ammonium availability and eelgrass (Zostera marina ) growth显示文摘 | Dennison W C Aller R C Alberte R S | 1987 | Marine Biology1987，94，3： | 1 |
| 14 | Isolation, chromosomal localization and differential expression of mitochondrial manganese superoxide dismutase and chloroplastic copper/zine superoxide dismutase genes in wheat显示文摘 | WU Guo-hai Ronald W W Albert J R | 1999 | Plant Pgysiology1999，120，： | 1 |
| 15 | Downey显示文摘 | Albert W Small P D Elizabeth A | 2001 | Managing Change: Some Important Aspects2001，，10： | 1 |
| 16 | Directed evolution of ionizing radiation resistance in Escherichia coli 显示文摘 | Harris D R Pollock S V Wood E A Goiffon R J Klingele A J Cabot E L Schackwitz W Martin J Eggington J Durfee T J Middle C M Norton J E Popelars M C Li H Klugman S A Hamilton L L Bane L B Pennacchio L A Albert T J Perna N T Cox M M Battista J R | 2009 | Journal of Bacteriology2009，191，16： | 1 |
| 17 | Intraspecific functional variability: extent, structure and sources of variation显示文摘 | Albert C H Thuiller W Yoccoz N G | 2010 | Journal of Ecology2010，98，3： | 1 |
| 18 | Intestinal farnesoid X receptor signaling promotes nonalcoholic fatty liver disease显示文摘 | Jiang Changtao Xie Cen Li Fei Zhang Limin Nichols Robert G Krausz Kristopher W Cai Jingwei Qi Yunpeng Fang Zhong-Ze Takahashi Shogo Tanaka Naoki Desai Dhimant Amin Shantu G Albert Istvan Patterson Andrew D Gonzalez Frank J | 2015 | Journal of Clinical Investigation2015，，1： | 1 |
| 19 | Dietary α- linolenic acid intake and risk of sudden cardiac death and coronary heart disease 显示文摘 | Albert CM Oh K Wbang W | 2005 | Am Heart J2005，112，21： | 1 |
| 20 | Nitrogen and Phosphorted by Eroded Soil Aggregates显示文摘 | Alberts E E Moldenhauer W C | 1981 | Soil Sci Soc A m J1981，45，： | 1 |
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