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| 1 | Adipose-derived mesenchymal stem cell transplantation promotes adult neurogenesis in the brains of Alzheimer's disease mice显示文摘In the present study,we transplanted adipose-derived mesenchymal stem cells into the hippocampi of APP/PS1 transgenic Alzheimer’s disease model mice.Immunofluorescence staining revealed that the number of newly generated(BrdU+)cells in the subgranular zone of the dentate gyrus in the hippocampus was significantly higher in Alzheimer’s disease mice after adipose-derived mesenchymal stem cell transplantation,and there was also a significant increase in the number of BrdU+/DCX+neuroblasts in these animals.Adipose-derived mesenchymal stem cell transplantation enhanced neurogenic activity in the subventricular zone as well.Furthermore,adipose-derived mesenchymal stem cell transplantation reduced oxidative stress and alleviated cognitive impairment in the mice.Based on these findings,we propose that adipose-derived mesenchymal stem cell transplantation enhances endogenous neurogenesis in both the subgranular and subventricular zones in APP/PS1 transgenic Alzheimer’s disease mice,thereby facilitating functional recovery. | Yufang Yan Tuo Ma Kai Gong Qiang Ao Xiufang Zhang Yao Gong | 2014 | Neural Regeneration Research2014，9，8： | 16 |
| 2 | Second-look arthroscopic evaluation of the articular cartilage after primary single-bundle and double-bundle anterior cruciate ligament reconstructions显示文摘在前面的十字形的系带( ACL ) reconstruction.No 报告关于比较变化在以后的软骨被出版了以后,背景几报告显示出关节的软骨退化的前进单个捆( SB )并且这研究的双捆( DB ) ACL reconstructions.The 目的是在 SB 和 DB ACL 以后评估关节的软骨变化由收到了 arthroscopic ACL 重建的 99 个病人回顾地是的秒看 arthroscopy.Methods | WANG Hai-jun AO Ying-fang CHEN Lian-xu GONG Xi WANG Yong-jian MA Yong LEUNG Kevin Kar Ming YU Jia-kuo | 2011 | Chinese Medical Journal2011，，21： | 12 |
| 3 | Effects of multidrug resistance, antisense RNA on the chemosensitivity of hepatocellular carcinoma cells显示文摘BACKGROUND: Multidrug resistance is a major obstacle in cancer chemotherapy. We examined whether the antisense RNA of multidrug resistance gene 1 (mdr1) could reverse multidrug resistance in the human hepatocellular carcinoma (HCC) cell line SMMC7721/ADM. METHODS: The recombinant adenoviruses pAdEasy- GFP-ASmdr1 product was produced by the adenoviral vector AdEasy system, which can express antisense RNA against the mdr1 gene. Following that, the recombinant adenovirus was transfected into the P-glycoprotein- producing multidrug resistance cell line, SMMC7721/ADM human HCC cells resistant to adriamycin (ADM) and daunorubicin (DNR). In order to investigate the reversal of multidrug resistance phenotype, we measured the expression of mdr1 mRNA by RT-PCR and the production of P-glycoprotein by flow cytometry. The sensitivities for ADM and DNR SMMC7721/ADM cells were examined by [3-(4, 5-dimethylthi-azol-2-yl)-2,5 diphenyl-terazolium bromide] (MTT) analysis. RESULTS: The low-level expression of mdr1 mRNA and P-glycoprotein production were observed in parental sensitive cells SMMC/7721 in addition to the overexpressionof mdr1 mRNA and P-glycoprotein in SMMC7721/ADM cells. The transfection of antisense-RNA into SMMC7721/ ADM cells resulted in decreases of mdr1 mRNA and P-glycoprotein, but increase of drug sensitivities. The sensitivities of transfected SMMC7721/ADM cells to ADM and DNR in IC50 reduced by 31.25% and 62.96% respectively. CONCLUSIONS: Mdr1 antisense RNA can increase the sensitivities of SMMC7721/ADM cells to anticancer drug by decreasing the expression of the mdr1 gene and inhibiting P-glycoprotein expression. This strategy may be applicable to cancer patients with P-glycoportein mediated multidrug resistance. | Bo Li, Tian Ye, Lei Zhao, De-Hua Li, Xing-Hua Gou, Lan-Ying Zhao, Lei Han, Lin Chen, Lu-Nan Yan and Jian-Ping Gong Department of Hepatobiliary Surgery, Second Affiliated Hospital, Chongqing University of Medical Sciences, Chongqing 400010, China Department of Traditional Chinese Surgery, Second Affiliated Hospital, Luzhou Medical College, Luzhou 646000, China Genetic Engineering Laboratory, Chengdu Di’ao Group Co., Ltd., Chengdu 610041, China Department of General Surgery, West China Hospital, Sichuan University, Chengdu 610041, China | 2006 | Hepatobiliary & Pancreatic Diseases International2006，5，4： | 10 |
| 4 | Effects of inorganic arsenic on growth and microcystin production of a Microcystis strain isolated from an algal bloom in Dianchi Lake,China显示文摘Our previous data have shown that inorganic arsenic concentrations were high in Dianchi Lake,China,where Microcystis blooms often occur.To explore the relationship between arsenic and the growth of Microcystis,the effects of arsenite[As(III)]and arsenate[As(V)]on the growth and toxin production of M.aeruginosa strain FACHB 905 were tested.Results showed that M.aeruginosa FACHB 905 was tolerant to inorganic arsenic and its growth was not inhibited when the concentration of As(III)was below 10-5 mol L-1 or that of As(V)below 10-3 mol L-1.Total microcystin production was stimulated in the presence of 10-7 mol L-1 As(III)and the response of this M.aeruginosa strain to As(III)seemed to be a typical inverted U-shaped hormesis.The content increase of microcystin-LR per cell indicated that the toxicity was enhanced as M.aeruginosa FACHB 905 was exposed to As(V).Considering the relatively high concentration of inorganic arsenic in Dianchi Lake(139μg L-1 in epilimnetic water),the origin of the M.aeruginosa strain,inorganic arsenic favors survival of M.aeruginosa FACHB 905 and may stimulate its microcystin production and cellular toxicity. | GONG Yan AO HongYi LIU BiBo WEN Sheng WANG Zhi HU DingJing ZHANG XingZhong SONG LiRong LIU JianTong | 2011 | Chinese Science Bulletin2011，56，22： | 6 |
| 5 | Comparative study on early period of recovery between minimally invasive surgery total knee arthroplasty and minimally invasive surgery-quadriceps sparing total knee arthroplasty in Chinese patients显示文摘最低限度地侵略的外科(MIS ) 的背景不同类型过程现在在全部的膝关节造形术(TKA ) 被使用了。与有一个长皮肤切口的传统的 TKA 过程相比,临床的研究证明 MIS 过程有一些优点。四头肌 sparing (QS ) 过程是最最低限度地侵略的 MIS 过程直到现在。这研究被瞄准为中国病人的 vastus medialis 发现插入类型,如果 QS 过程在病人在2006年2月和2007年5月之间的早 recovery.Methods 有一些优点, 120 个连续病人在一般麻醉, 14 个病人在之中被失去到后续下面经历了单方的主要 TKA ,留下 106 个盒子在这研究被注册。在 106 个盒子之中,有 85 右膝, 21 左膝(15 个男人和 91 个女人,与 65.1 的吝啬的年龄 ???? ￡吗? | YU Jia-kuo YU Chang-long AO Ying-fang GONG Xi WANG Yong-jian WANG Shu XING Xie CHEN Lian-xu JU Xiao-dong | 2008 | Chinese Medical Journal2008，，15： | 5 |
| 6 | Amyloid beta-peptide worsens cognitive impairment following cerebral ischemia-reperfusion injury显示文摘Amyloid β-peptide,a major component of senile plaques in Alzheimer’s disease,has been implicated in neuronal cell death and cognitive impairment.Recently,studies have shown that the pathogenesis of cerebral ischemia is closely linked with Alzheimer’s disease.In this study,a rat model of global cerebral ischemia-reperfusion injury was established via occlusion of four arteries;meanwhile,fibrillar amyloidβ-peptide was injected into the rat lateral ventricle.The Morris water maze test and histological staining revealed that administration of amyloidβ-peptide could further aggravate impairments to learning and memory and neuronal cell death in the hippocampus of rats subjected to cerebral ischemia-reperfusion injury.Western blot showed that phosphorylation of tau protein and the activity of glycogen synthase kinase 3βwere significantly stronger in cerebral ischemia-reperfusion injury rats subjected to amyloid β-peptide administration than those undergoing cerebral ischemia-reperfusion or amyloidβ-peptide administration alone.Conversely,the activity of protein phosphatase 2A was remarkably reduced in rats with cerebral ischemia-reperfusion injury following amyloidβ-peptide administration.These findings suggest that amyloidβ-peptide can potentiate tau phosphorylation induced by cerebral ischemia-reperfusion and thereby aggravate cognitive impairment. | Bo Song Qiang Ao Ying Niu Qin Shen Huancong Zuo Xiufang Zhang Yandao Gong | 2013 | Neural Regeneration Research2013，8，26： | 5 |
| 7 | Phosphorylation of tau protein over time in rats subjected to transient brain ischemia显示文摘Transient brain ischemia has been shown to induce hyperphosphorylation of the microtubule-associated protein tau.To further determine the mechanisms underlying these processes,we investigated the interaction between tau,glycogen synthase kinase(GSK)-3βand protein phosphatase 2A.The results confirmed that tau protein was dephosphorylated during brain ischemia;in addition,the activity of GSK-3βwas increased and the activity of protein phosphatase2A was decreased.After reperfusion,tau protein was hyperphosphorylated,the activity of GSK-3βwas decreased and the activity of protein phosphatase 2A remained low.Importantly,the interaction of tau with GSK-3βand protein phosphatase 2A was altered during ischemia and reperfusion Lithium chloride could affect tau phosphorylation by regulating the interaction of tau with GSK-3βand protein phosphatase 2A,and improve learning and memory ability of rats after transient brain ischemia.The present study demonstrated that it was the interaction of tau with GSK-3βand protein phosphatase 2A,rather than their individual activities,that dominates the phosphorylation of tau in transient brain ischemia.Hyperphosphorylated tau protein may play an important role in the evolution of brain injury in ischemic stroke.The neuroprotective effects of lithium chloride partly depend on the inhibition of tau phosphorylation during transient brain ischemia. | Bo Song Qiang Ao Zhen Wang Weiqiang Liu Ying Niu Qin Shen Huancong Zuo Xiufang Zhang Yandao Gong | 2013 | Neural Regeneration Research2013，8，34： | 2 |
| 8 | Zircon U-Pb ages and Hf isotopic characteristics of the Dehe biotite monzonitic gneiss pluton in the North Qinling orogen and their geological significance显示文摘The Dehe granitic pluton intruded the Xiahe Group which is in the core complex of the North Qinling Orogenic Belt(NQOB).It shows gneissic bedding and possesses typical S-type granite minerals such as muscovite and garnet.LA-ICP-MS U-Pb isotopic dating of the Dehe granite yielded a weighted average age of 925±23 Ma which represents the emplacement age of the pluton.Most of the εHf(t) values are negative,and the two-stage model ages are consistent with the age of the Qinling Group.The isotope data show that the Dehe granite was formed in the following geological setting:in the syn-collision setting of the NQOB in the Neoproterozoic,crustal thickening induced partial melting of materials derived from the Qinling complex,and then the maga upwelled and intruded into the Xiahe Group.The formation of the Dehe S-type granite implied the occurrence of a convergent event in the QOB during the Neoproterozoic. | Wang, Fei Zhu, Laimin Li, Jiming Lee, Ben Gong, Hujun Yang, Tao Wang, Wei Xu, Ao | 2011 | Chinese Journal Of Geochemistry2011，30，2： | 1 |
| 9 | Engineering microparticles based on solidified stem cell secretome with an augmented pro-angiogenic factor portfolio for therapeutic angiogenesis显示文摘Tissue (re)vascularization strategies face various challenges, as therapeutic cells do not survive long enough in situ, while the administration of pro-angiogenic factors is hampered by fast clearance and insufficient ability to emulate complex spatiotemporal signaling. Here, we propose to address these limitations by engineering a functional biomaterial capable of capturing and concentrating the pro-angiogenic activities of mesenchymal stem cells (MSCs). In particular, dextran sulfate, a high molecular weight sulfated glucose polymer, supplemented to MSC cul-tures, interacts with MSC-derived extracellular matrix (ECM) components and facilitates their co-assembly and accumulation in the pericellular space. Upon decellularization, the resulting dextran sulfate-ECM hybrid material can be processed into MIcroparticles of SOlidified Secretome (MIPSOS). The insoluble format of MIPSOS protects protein components from degradation, while facilitating their sustained release. Proteomic analysis demonstrates that MIPSOS are highly enriched in pro-angiogenic factors, resulting in an enhanced pro-angiogenic bioactivity when compared to naïve MSC-derived ECM (cECM). Consequently, intravital microscopy of full-thickness skin wounds treated with MIPSOS demonstrates accelerated revascularization and healing, far superior to the ther-apeutic potential of cECM. Hence, the microparticle-based solidified stem cell secretome provides a promising platform to address major limitations of current therapeutic angiogenesis approaches. | Thomas Spater Marisa Assunçao Kwok Keung Lit Guidong Gong Xiaoling Wang Yi-Yun Chen Ying Rao Yucong Li Chi Him Kendrick Yiu Matthias W.Laschke Michael D.Menger Dan Wang Rocky S.Tuan Kay-Hooi Khoo Michael Raghunath Junling Guo Anna Blocki | 2022 | Bioactive Materials2022，7，11： | 1 |
| 10 | Synergistic effect of neural stem cells and olfactory ensheathing cellss on repair of adult rat spinal Cord Injury显示文摘 | Wang G Ao Q Gong K | | 0，，10： | 1 |
| 11 | Characterization of gene rear- rangements resulted from genomic structural aberrations in human esophageal squamous cell carcinoma KYSE150 cells显示文摘 | H ao J J Gong T Zhang Y | 2013 | Gene2013，513，1： | 1 |
| 12 | Double-bundle anterior eru- ciate ligament reconstruction using bone-patellar tendon-bo- neallograft :technique and 2- to 5-year follow-up 显示文摘 | Jiang D Ao YF Gong X | 2012 | Am J Sports Med2012，40，5： | 1 |
| 13 | Supported Lal_xCexMn03 perovskite catalysts: preparation, characterization and catalytic performance in methane combustion显示文摘 | Ying SHAO Xiao-Fei WANG Min AO Cai-Rong GONG Guo-Liang FAN Hai-Feng CHEN | 2012 | Frontiers of Materials Science2012，6，4： | 1 |
| 14 | Remnant-preserving posterior cruciate ligament reconstruction with graft tension-relieving: a comparative study with conventional technique显示文摘 | GONG Xi YU Jia-kuo AO Ying-fang | 2013 | Chinese Medical Journal2013，，6： | 1 |
| 15 | Highly oriented perpendicular Co-alloy media on Si ( 111 ) suhstrates 显示文摘 | Gong H P ao M Laughlin D E | 1999 | J Appl Phys1999，85，8： | 1 |
| 16 | The effect of topology of chitosan biomaterials on the differentiation and proliferation of neural stem cells显示文摘 | WANG G AO Q GONG K | 2010 | Acta Biomater2010，6，9： | 1 |
| 17 | Preparation and characterization of chitosan-heparin composite matrices for blood contacting tissue engineering显示文摘 | He Q Ao Q Gong K | | 0，，05： | 1 |
| 18 | Synergistic effect of neural stem cells and olfactory ensheathing cells on repair of adult rat spinal cord injury显示文摘 | Wang G Ao Q Gong K | | 0，，10： | 1 |
| 19 | Physical, mechanical and degradation properties, and Schwann cell affinity of cross-linked chitosan films显示文摘 | Wenling Cao Mingyu Cheng Qiang Ao Yandao Gong Nanming Zhao Xiufang Zhang | 2005 | Journal of Biomaterials Science Polymer Edition2005，，6： | 1 |
| 20 | Positive charge of chitosan retards blood coagulation on chitosan films显示文摘 | He Q Gong K Ao Q | | 0，，： | 1 |
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