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| 1 | The First Imported Case of Monkeypox in the Mainland of China—Chongqing Municipality,China,September 16,2022显示文摘Monkeypox is a zoonotic viral disease caused by the monkeypox virus(MPXV),and historically,all outbreaks have been linked to Africa;however,monkeypox has been posing an alarming challenge to the world in 2022(1)as approximately 60,000 cases have been reported in more than 100 nations and regions worldwide(2).Currently,many cases of monkeypox were identified in many nonendemic countries outside of Central and West Africa,and human-to-human transmission has occurred frequently,especially among men who have sex with men(MSM)presenting new clinical symptoms similar to syphilis and other sexually transmitted infections(3). | Hua Zhao Wenling Wang Li Zhao Sheng Ye Jingdong Song Roujian Lu Hua Zong Changcheng Wu Wei Huang Baoying Huang Yao Deng Ruhan A Wujuan Xie Li Qi Wenbo Xu Hua Ling Wenjie Tan | 2022 | China CDC weekly2022，4，38： | 35 |
| 2 | The structural and accessory proteins M,ORF 4a,ORF 4b,and ORF 5 of Middle East respiratory syndrome coronavirus(MERS-CoV)are potent interferon antagonists显示文摘The newly emerged Middle East respiratory syndrome coronavirus(MERS-CoV)is a highly pathogenic respira-tory virus with pathogenic mechanisms that may be driven by innate immune pathways.The goal of this study is to characterize the expression of the structural(S,E,M,N)and accessory(ORF 3,ORF 4a,ORF 4b,ORF 5)proteins of MERS-CoV and to determine whether any of these pro-teins acts as an interferon antagonist.Individual structural and accessory protein-coding plasmids with an N-terminal HA tag were constructed and transiently transfected into cells,and their native expression and subcellular localiza-tion were assessed using Wes tern blotting and indirect immunofl uorescence.While ORF 4b demonstrated majorly nuclear localization,all of the other proteins demonstrated cytoplasmic localization.In addition,for the fi rst time,our experiments revealed that the M,ORF 4a,ORF 4b,and ORF 5 proteins are potent interferon antagonists.Further exami-nation revealed that the ORF 4a protein of MERS-CoV has the most potential to counteract the antiviral effects of IFN via the inhibition of both the interferon production(IFN-βpromoter activity,IRF-3/7 and NF-κB activation)and ISRE promoter element signaling pathways.Together,our re-sults provide new insights into the function and pathogenic role of the structural and accessory proteins of MERS-CoV. | Yang Yang Ling Zhang Heyuan Geng Yao Deng Baoying Huang Yin Guo Zhengdong Zhao Wenjie Tan | 2013 | Protein & Cell2013，4，12： | 25 |
| 3 | Age-related rhesus macaque models of COVID-19显示文摘Background:Since December 2019,an outbreak of the Corona Virus disease 2019(COVID-19)caused by severe acute respiratory syndrome coronavirus(SARS-CoV-2)in Wuhan,China,has become a public health emergency of international concern.The high fatality of aged cases caused by SARS-CoV-2 was a need to explore the possible age-related phenomena with non-human primate models.Methods:Three 3-5 years old and two 15 years old rhesus macaques were intratracheally infected with SARS-CoV-2,and then analyzed by clinical signs,viral replication,chest X-ray,histopathological changes and immune response.Results:Viral replication of nasopharyngeal swabs,anal swabs and lung in old monkeys was more active than that in young monkeys for 14 days after SARS-CoV-2 challenge.Monkeys developed typical interstitial pneumonia characterized by thickened alveolar septum accompanied with inflammation and edema,notably,old monkeys exhibited diffuse severe interstitial pneumonia.Viral antigens were detected mainly in alveolar epithelial cells and macrophages.Conclusion:SARS-CoV-2 caused more severe interstitial pneumonia in old monkeys than that in young monkeys.Rhesus macaque models infected with SARS-CoV-2 provided insight into the pathogenic mechanism and facilitated the development of vaccines and therapeutics against SARS-CoV-2 infection. | Pin Yu Feifei Qi Yanfeng Xu Fengdi Li Peipei Liu Jiayi Liu Linlin Bao Wei Deng Hong Gao Zhiguang Xiang Chong Xiao Qi Lv Shuran Gong Jiangning Liu Zhiqi Song Yajin Qu Jing Xue Qiang Wei Mingya Liu Guanpeng Wang Shunyi Wang Haisheng Yu Xing Liu Baoying Huang Wenling Wang Li Zhao Huijuan Wang Fei Ye Weimin Zhou Wei Zhen Jun Han Guizhen Wu Qi Jin Jianwei Wang Wenjie Tan Chuan Qin | 2020 | Animal Models and Experimental Medicine2020，3，1： | 22 |
| 4 | The endoplasmic reticulum-associated degradation is necessary for plant salt tolerance显示文摘真核细胞的有机体有允许错误褶层或 unassembled 蛋白质在 endoplasmic 蜂窝胃被保留的质量控制的机制(嗯) 并且随后由嗯联系降级(ERAD ) 降级了。ERAD 小径很好在酵母和哺乳动物被学习;然而,植物 ERAD 的生物功能没被报导。通过分子、细胞的生物途径,我们发现 ERAD 是必要的让植物克服盐应力。在蛋白质在植物房间增加了的盐处理 ubiquitinated 之上,特别展开了快速在 ER 积累了并且随后导致了的蛋白质嗯压力回答。在 ERAD 小径的 HRD1/HRD3 建筑群的 HRD3A 的缺点导致了展开的蛋白质反应(UPR ) 的改变,腌的增加的植物敏感,和在植物房间的 ERAD 底层的保留。而且,我们证明从 ER 的那个 Ca 2+ 版本涉及 UPR 的举起,反应的氧种类(ROS ) 参与 ERAD 相关的植物盐反应小径。 | Lijing Liu Feng Cui Qingliang Li Bojiao Yin Huawei Zhang Baoying Lin Zaorong Wu Ran Xia Sanyuan Tang Qi Xie | 2011 | Cell Research2011，21，6： | 19 |
| 5 | COVID-19 Outbreak Caused by Contaminated Packaging of Imported Cold-Chain Products—Liaoning Province,China,July 2020显示文摘Summary What is known about this topic?Few major outbreaks of coronavirus disease 2019(COVID-19)have occurred in China after major nonpharmaceutical interventions and vaccines have been deployed and implemented.However,sporadic outbreaks that had high possibility to be linked to cold chain products were reported in several cities of China..What is added by this report?In July 2020,a COVID-19 outbreak occurred in Dalian,China.The investigations of this outbreak strongly suggested that the infection source was from COVID-19 virus-contaminated packaging of frozen seafood during inbound unloading personnel contact.What are the implications for public health practice?Virus contaminated paper surfaces could maintain infectivity for at least 17–24 days at-25℃.Exposure to COVID-19 virus-contaminated surfaces is a potential route for introducing the virus to a susceptible population.Countries with no domestic transmission of COVID-19 should consider introducing prevention strategies for both inbound travellers and imported goods.Several measures to prevent the introduction of the virus via cold-chain goods can be implemented. | Huilai Ma1 Jianqun Zhang Ji Wang Ying Qin Cao Chen Yang Song Liang Wang Jun Meng Lingling Mao Fengqin Li Ning Li Jian Cai Yong Zhang Dayan Wang Yunting Xia Hong Wang Shaofeng Jiang Xiang Zhao Peihua Niu Wenjie Tan Tao Ma Yecheng Yao Naiying Mao Zhen Zhu Tianjiao Ji Qian Yang Baoying Huang Li Zhao Jianxing Yu Li Bai Shuangli Zhu Dongyan Wang Yan Zhang Yingwei Sun Mingchun Luan Yanhai Wang Haibo Sun Shihong Yang Zhijian Bo Xiang Ren Zhongjie Li George Fu Gao Wei Yao Wenqing Yao Zijian Feng Wenbo Xu | 2021 | China CDC weekly2021，3，21： | 16 |
| 6 | Changes of Temperature and Precipitation Extremes in Hengduan Mountains,Qinghai-Tibet Plateau in 1961-2008显示文摘Variations and trends in extreme climate events are more sensitive to climate change than the mean values,and so have received much attention.In this study,twelve indices of temperature extremes and 11 indices of precipitation extremes at 32 meteorological stations in Hengduan Mountains were examined for the period 1961-2008.The results reveal statistically significant increases in the temperature of the warmest and coldest nights and in the frequencies of extreme warm days and nights.Decreases of the diurnal temperature range and the numbers of frost days and ice days are statistically significant.Regional averages of growing season length also display the trends consistent and significant with warming.At a large proportion of the stations,patterns of temperature extremes are consistent with warming since 1961:warming trends in minimum temperature indices are greater than those relating to maximum temperature.As the center of the Shaluli Mountain,the warming magnitudes decrease from inner to outer.Changes in precipitation extremes is low:trends are difficult to detect against the larger inter-annual and decadal-scale variability of precipitation,and only the wet day precipitation and the regional trend in consecutive dry days are significant at the 0.05 level.It can be concluded that the variation of extreme precipitation events is not obvious in the Hengduan Mountains,however,the regional trends generally decrease from the south to the north.Overall,the spatial distribution of temporal changes of all extreme climate indices in the Hengduan Mountains illustrated here reflects the climatic complexity in mountainous regions. | NING Baoying YANG Xiaomei CHANG Li | 2012 | Chinese Geographical Science2012，22，4： | 14 |
| 7 | Source of major anions and cations of snowpacks in Hailuogou No.1 glacier, Mt. Gongga and Baishui No.1 glacier, Mt. Yulong显示文摘积雪场样品是从二条冰河的 colleted:在 2006 年 6 月的 Baishui No.1 冰河和 Hailuogou No.1 冰河。海盐离子 tracer。 | LI Zongxing HE Yuanqing PANG Hongxi YANG Xiaomei JIA Wenxiong ZHANG Ningning WANG Xufeng NING Baoying YUAN Lingling SONG Bo | 2008 | Journal of Geographical Sciences2008，18，1： | 13 |
| 8 | Small hairpin RNA targeting at Bcl-2 increases cytarabine-induced apoptosis in Raji cells显示文摘Bcl-2, Bcl-2 家庭蛋白质的一个突出的成员,为 apoptosis 和抵抗的 dysregulation 负责到化疗。在这研究,我们调查了在 Bcl-2 mRNA 指向的小发卡 RNA (shRNA ) 是否能提高导致的 cytarabine (Ara-C ) 在 Raji 房间的 apoptosis。Bcl-2 shRNA 是进 Raji 房间和 Bcl-2 mRNA 的表示层次的 transfected,蛋白质是由 RTPCR 和 immunofluorescence 的 assayed。房间增长被 MTT 试金决定。Apoptosis 被词法观察和流动 cytometric 分析决定。我们表示与 Bcl-2 shRNA 从 Raji 房间 transfected Bcl-2 mRNA 和蛋白质铺平的结果表演减少了,与否定控制 shRNA 组或 untransfected 房间组织的任何一个相比(P < 0.05 ) 。有 Bcl-2 shRNA 的房间 transfected 的生存能力是不到有控制 shRNA 和 untransfected Raji 房间的房间 transfected,分别地(P < 0.05 ) 。显著地与 Ara-C 相结合的 Bcl-2 shRNA 禁止了细胞的生长(P < 0.05 ) 。在在独自与 Ara-C 对待的控制 shRNA/Ara-C 联合和房间之间的房间幸存没有差别。用染色的 Giemsa,在 48 h 加 Ara-C 与 Bcl-2 shRNA 对待的房间显示了 apoptosis 的变化。对待, Bcl-2 shRNA 显著地与 Ara-C 结合了的 Raji 房间的 Apoptotic 率增加了(P < 0.05 ) ,与任何一个控制 shRNA/Ara-C 相比联合或 Ara-C-treated 房间独自一个。我们的结果建议对 Bcl-2 mRNA 的 shRNA 能在 Raji 房间增加 Ara-C-induced apoptosis。 | Dongmei He Baoying Fang | 2009 | Acta Biochimica et Biophysica Sinica2009，41，2： | 9 |
| 9 | Synoptic-scale variation of δ^(18)O in summer monsoon rainfall at Lijiang, China显示文摘Based on the daily δ^(18)O data in June―September 2003 at Lijiang and the daily mean NCEP/ NCAR reanalysis data, synoptic-scale variation of δ^(18)O in summer monsoon rainfall was investigated. The 'precipitation amount effect' is obvious for the daily δ^(18)O variation, whereas the 'temperature effect' is insignificant. Alternate occurrences of active phase and break phase of the southwest monsoon probably influence the synoptic-scale δ^(18)O variation prominently. Moreover, the isotopic composition in precipitation during the late monsoon months is presumably influenced significantly by recycling of monsoon precipitation. Both the above factors disturb the 'amount effect' of isotopic variation in the monsoon region. This study also indicates that the synoptic-scale rainfall δ^(18)O variation at Lijiang in summer is domi-nated by the Indian monsoon depression (low pressure) system at large scale. These results are important for further studying the 'amount effect' and reconstructing paleoclimate in the monsoon region. | PANG Hongxi HE Yuanqing LU Aigang ZHAO Jingdong NING Baoying YUAN Lingling SONG Bo | 2006 | Chinese Science Bulletin2006，51，23： | 9 |
| 10 | Three Novel Real-Time RT-PCR Assays for Detection of COVID-19 Virus显示文摘What is already known on this topic?A novel human coronavirus,known as SARS-CoV-2 or 2019-nCoV,is the causative agent of the coronavirus disease 2019(COVID-19).We have released the primers and probes of real-time reverse transcription polymerase chain reaction(rRT-PCR)assays for the laboratory detection of COVID-19 infection.What is added by this report?Here we provide detailed technical data and evaluate the performance of three novel rRT-PCR assays targeting the ORF1ab,N,and E genes for detection of COVID-19 infection.The application of rRT-PCR assays among four types of specimens(alveolar lavage,sputum,throat swabs,and stool)from patients with COVID-19 indicated that the mean viral loads detected in sputum were higher than other specimens.What are the implications for public health practice?These rRT-PCR assays reported here could be used for laboratory diagnosis of COVID-19 infection with high sensitivity,specificity,and applicability.Sputum rather than throat swabs and stool should be a priority for specimen collection for laboratory detection of COVID-19. | Peihua Niu Roujian Lu Li Zhao Huijuan Wang Baoying Huang Fei Ye Wenling Wang Wenjie Tan | 2020 | China CDC weekly2020，2，25： | 8 |
| 11 | Enhanced dewatering characteristics of waste activated sludge with Fenton pretreatment: effectiveness and statistical optimization显示文摘在这个工作,废物的提高的 dewaterabing 特征用 Fenton 激活污泥预告的处理以有效性和统计优化被调查。反应表面方法( RSM )和中央合成设计(电荷耦合器件)被使用评估并且优化重要运作的参数的有效性,即, H <潜水艇class=“ a-plus-plus ”> 2 O <潜水艇class=“ a-plus-plus ”> 2 集中, Fe <啜class=“ a-plus-plus ”>2+集中和起始的 pH 价值。一个重要二次的多项式模型被获得(R < 啜 class= “ a-plus-plus ” > 2 = 0.9189 ) 与毛状的吸时间(CST ) 减小效率作为反应。数字优化基于愿望功能被执行。为 H 的最佳价值 < 潜水艇 class= “ a-plus-plus ” > 2 O < 潜水艇 class= “ a-plus-plus ” > 2 , Fe < 啜 class= “ a-plus-plus ” >2+, 和起始的 pH 被发现是 178 mg?? | Guangyin ZHEN Xueqin LU Baoying WANG Youcai ZHAO Xiaon CHAI Dongjie NIU Tiantao ZAO | 2014 | Frontiers of Environmental Science & Engineering2014，8，2： | 7 |
| 12 | Research of contour error compensation control for X-C non-circular grinding in polar coordinates显示文摘In the X-C linkage grinding of non-circular parts,the computation and control method of contour error in polar coordinates platform is different with that in the XY coordinates platform.To solve this problem,the analysis of the definition and computation methods for contour error and track error in polar coordinates platform will be made.Through the relative lead-lag relation of the linkage axes in the grinding process,the range of the estimation contour error is narrowed and a contour error calculation model is constructed.Then the contour compensation controllers along contour error direction and trajectory tracking error direction are designed respectively,and the error compensation decoupling matrix of the X-C linkage axes is given as well.In the end of this paper,we take the machining of the cylinder contour in Wankel rotary piston engine as an example.A simulation experiment of contour error compensation control based on relative lead-lag quantity is made.The result shows that the designed contour compensation controller can increase the contour machining accuracy effectively. | LI Qiguang HAN Qiushi PENG Baoying WANG Hongjun | 2014 | Instrumentation2014，1，1： | 6 |
| 13 | Development and effectiveness of pseudotyped SARS-CoV-2 system as determined by neutralizing efficiency and entry inhibition test in vitro显示文摘With the development of the COVID-19 epidemic,there is an urgent need to establish a system for determining the effectiveness and neutralizing activity of vaccine candidates in biosafety level 2(BSL-2)facilities.Previously,researchers had developed a pseudotyped virus systemfor SARS-CoV andMERS-CoV,based onHIV-1 core,bearing virus spike protein.During the development of a pseudotyped SARS-CoV-2 system,a eukaryotic expression plasmid expressing SARSCoV-2 spike(S)protein was constructed and then co-transfectedwith HIV-1 based plasmid which containing the firefly luciferase reporter gene,into HEK293T cells to prepare the pseudotyped SARS-CoV-2 virus(ppSARS-2).We have successfully established the pseudotyped SARS-CoV-2 system for neutralization and entry inhibition assays.Huh7.5 cell line was found to be the most susceptible to our pseudotyped virus model.Different levels of neutralizing antibodies were detected in convalescent serum samples of COVID-19 patients using ppSARS-2.The recombinant,soluble,angiotensin-converting enzyme 2 protein was found to inhibit the entry of ppSARS-2 in Huh7.5 cells effectively.Furthermore,the neutralization results for ppSARS-2 were consistent with those of live SARS-CoV-2 and determined using the serum samples fromconvalescent patients.In conclusion,we have developed an easily accessible and reliable tool for studying the neutralizing efficiency of antibodies against SARS-CoV-2 and the entry process of the virus in a BSL-2 laboratory. | Ren Yang Baoying Huang Ruhan A Wenhui Li Wenling Wang Yao Deng Wenjie Tan | 2020 | Biosafety and Health2020，2，4： | 5 |
| 14 | DNA sensing and associated type 1 interferon signaling contributes to progression of radiation-induced liver injury显示文摘Liver damage upon exposure to ionizing radiation(IR),whether accidental or therapeutic,can contribute to liver dysfunction.Currently,radiotherapy(RT)is used for various cancers including hepatocellular carcinoma(HCC);however,the treatment dose is limited by radiation-induced liver disease(RILD)with a high mortality rate.Furthermore,the precise molecular mechanisms of RILD remain poorly understood.Here,we investigated RILD pathogenesis using various knockout mouse strains subjected to whole-liver irradiation.We found that hepatocytes released a large quantity of double-stranded DNA(dsDNA)after irradiation.The cGAS-STING pathway in non-parenchymal cells(NPCs)was promptly activated by this dsDNA,causing interferon(IFN)-I production and release and concomitant hepatocyte damage.Genetic and pharmacological ablation of the IFN-I signaling pathway protected against RILD.Moreover,clinically irradiated human peri-HCC liver tissues exhibited substantially higher STING and IFNβexpression than non-irradiated tissues.Increased serum IFNβconcentrations post-radiation were associated with RILD development in patients.These results delineate cGAS-STING induced type 1 interferon release in NPCs as a key mediator of IR-induced liver damage and described a mechanism of innate-immunity-driven pathology,linking cGAS-STING activation with amplification of initial radiation-induced liver injury. | Shisuo Du Genwen Chen Baoying Yuan Yong Hu Ping Yang Yixing Chen Qianqian Zhao Jian Zhou Jia Fan Zhaochong Zeng | 2021 | Cellular & Molecular Immunology2021，18，7： | 4 |
| 15 | Lactic Acid Assisted Solvothermal Synthesis of BiOClxI1-x Solid Solutions as Excellent Visible Light Photocatalysts显示文摘A series of BiOClxI1-x(x=0,0.1,0.3,0.5,0.7,0.9,1.0)photocatalysts was firstly prepared by means of a facile solvothermal route with the help of lactic acid.The measured results show that the morphologies of the as-prepared samples are similar sheets with different thickness and diameters.Thinner nanosheets assembled flower-like BiOCI0.5I0.5 solid solution exhibited the highest photocatalytic activity and stability among the prepared samples for the degradation of methylene blue(MB)and methyl orange(MO)under the illumination of visible light.The excellent photocatalytic properties of BiOCI0.5I0.5 could be attributed to the high specific surface area,the suita-ble band gap energy and the lower recombination rate of the electrons and holes.In addition,catalyst BiOCI0.5I0.5 was further used to degradate a more complicated mixed dye(MCH-RhB+MB)system under visible light,displaying an excellent photocatalytic activity.Finally,the photocatalytic mechanism of catalyst BiOCI0.5I0.5 to degradate colorful dyes was proposed.The trapping experiments of active species indicated that the holes are the main active species for the degradation of the mixed dyes. | DUAN Chenglin SONG Jinling WANG Baoying LI Lun WANG Ruifen ZHANG Bangwen | 2019 | Chemical Research in Chinese Universities2019，35，2： | 4 |
| 16 | First Human Infection Case of Monkey B Virus Identified in China,2021显示文摘Monkey B virus(BV),initially isolated in 1932,is currently designated as Macacine alphaherpesvirus 1 by the International Committee on Taxonomy of Viruses(1).BV is an alphaherpesvirus enzootic in macaques of the genus Macaca,normally transmitted horizontally via direct contact and exchange of bodily secretions,just like herpes simplex virus(HSV)in humans. | Wenling Wang Wenjie Qi Jingyuan Liu Haijun Du Li Zhao Yang Zheng Guoxing Wang Yang Pan Baoying Huang Zhaomin Feng Daitao Zhang Peng Yang Jun Han Quanyi Wang Wenjie Tan | 2021 | China CDC weekly2021，3，29： | 4 |
| 17 | Autophagy occurs within an hour of adenosine triphosphate treatment after nerve cell damage:the neuroprotective effects of adenosine triphosphate against apoptosis显示文摘After hypoxia, ischemia, or inflammatory injuries to the central nervous system, the damaged cells release a large amount of adenosine triphosphate, which may cause secondary neuronal death. Autophagy is a form of cell death that also has neuroprotective effects. Cell Counting Kit assay, monodansylcadaverine staining, flow cytometry, western blotting, and real-time PCR were used to determine the effects of exogenous adenosine triphosphate treatment at different concentrations(2, 4, 6, 8, 10 mmol/L) over time(1, 2, 3, and 6 hours) on the apoptosis and autophagy of SH-SY5 Y cells. High concentrations of extracellular adenosine triphosphate induced autophagy and apoptosis of SH-SY5 Y cells. The enhanced autophagy first appeared, and peaked at 1 hour after treatment with adenosine triphosphate. Cell apoptosis peaked at 3 hours, and persisted through 6 hours. With prolonged exposure to the adenosine triphosphate treatment, the fraction of apoptotic cells increased. These data suggest that the SH-SY5 Y neural cells initiated autophagy against apoptosis within an hour of adenosine triphosphate treatment to protect themselves against injury. | Na Lu Baoying Wang Xiaohui Deng Honggang Zhao Yong Wang Dongliang Li | 2014 | Neural Regeneration Research2014，9，17： | 3 |
| 18 | Impacts of Yulong Mountain Glacier on Tourism in Lijiang显示文摘Yulong 山上的冰河是在丽江的最重要的吸引力之一,云南,中国。但是它不停地由于全球温暖撤退这些年,它肯定在丽江显著地影响地区性的旅游。这研究在在那里的旅游上估计 Yulong 山的冰河撤退的效果。原始资料包括人口分布通过来宾调查被收集,动机,停留时间,;关于到丽江的从旅游者的每个旅游资源的意见,以及关于舞台的点,他们访问了或将访问的问题,他们在他们的访问期间每天花费了多少;他们的访问行为怎么将在冰河将消失的假设之上被改变。这些数据被用来在丽江分析对旅游的冰河的贡献的比例;在地区性的旅游份量上上估计冰河的影响。根据调查,这能被结束四分之三的到丽江的旅游者对 Yulong 山冰河感兴趣,显示冰河为观光旅游者拥有著名呼吁。我们的分析的结果显示出那大约 689,013 ~1 , 508,247 个旅游者,为 19.63 ~4 的财务总数的 2.97 % 3,510,000 个国内旅游者到在 2004 的丽江,不当冰河不在时来到丽江,导致 84,382,508 ~ 的可能的直接经济损失 184,713,011 美金( viz 。700,374,824 ~ 1,533,117,993 RMB ) ;一显著地在吸引力半径减少。 | YUAN Lingling LU Aigang NING Baoying HE Yuanqing | 2006 | Journal of Mountain Science2006，3，1： | 3 |
| 19 | Identification of Histoplasma causing an unexplained disease cluster in Matthews Ridge, Guyana显示文摘Here,we report the identification of Histoplasma causing an unexplained disease cluster in Matthews Ridge,Guyana.In March 2019,14 employees of Chongqing Bosai Mining Company,China,working in a manganese mining of Guyana,had unexplained fever,and two of them died.We obtained lung and brain tissues as well as the blood samples from the two deceased cases(patient No.1 and 2),and bronchoscopy lavages and cerebrospinal fluid samples from one severe case(patient No.3),respectively.All samples were tested by pathological examination,high-throughput sequencing,and real-time PCR.Pathological detection showed the presence of spore-like structures in the lung tissue of patient No.1,indicating a fungal infection in this patient.Nanopore sequencing identified the existing of H.capsulatum in the lung tissue sample within 13 h.Next-generation sequencing identified specific fragments of H.capsulatum in all of the samples tested(lung,brain and blood serum from the deceased cases,and plasma from the severe case).Real-time PCR assays did not reveal any viral infection related to transmission from bat feces.We conclude that H.capsulatum was the causative pathogen of this disease cluster based on epidemiologic,clinical,pathological and nucleic acid evidence. | Ji Wang Weimin Zhou Hua Ling Xiaoping Dong Yi Zhang Jiandong Li Yong Zhang Jingdong Song William J.Liu Yang Li Ruiqing Zhang Wei Zhen Kun Cai Shuangli Zhu Dongyan Wang Jinbo Xiao Yigang Tong Wenli Liu Lihua Song Wei Wu Yang Liu Xiang Zhao Ruihuan Wang Sheng Ye Jing Wang Roujian Lu Baoying Huang Fei Ye Wenwen Lei Rongbao Gao Qi Shi Cao Chen Jun Han Wenbo Xu George F.Gao Xuejun Ma Guizhen Wu | 2019 | Biosafety and Health2019，1，3： | 3 |
| 20 | Isolation and Characterization of Monkeypox Virus from the First Case of Monkeypox—Chongqing Municipality,China,2022显示文摘Introduction:The first imported case of monkeypox(MPX)from the mainland of China was reported in September 2022.Herein,the study reports the isolation and characterization of MPX virus(MPXV)in this case.Methods:Clinical specimens including skin blister fluid,oropharyngeal and nasopharyngeal swabs,and blood were collected and inoculated onto Vero cells.The isolated virus was identified as MPXV using quantitative polymerase chain reaction(qPCR),cytopathic effects(CPEs),immunofluorescence assay(IFA)and transmission electron microscopy(TEM).Plaque assays were employed to quantify infectious plaque-forming units(PFUs).The plaque reduction neutralization test(PRNT)was developed to determine the neutralizing antibody(nAb)against MPXV.Results:MPXV replication was confirmed with qPCR.Typical CPEs were observed 48 h postincubation.The isolated virus was named MPXV-B.1-China-C-Tan-CQ01.IFA showed that MPXV reacted with serum of MPX case.Orthopoxvirus morphology was observed using TEM.The virus titer increased to>106 PFU/mL after three passages.The serum PRNT 50%neutralization titer(NT50)was 35 for the MPX patient 6 days after symptom onset.Discussion:The study successfully isolated the first MPXV strain in the mainland of China,MPXV-B.1-China-C-Tan-CQ01.Infectious titration and PRNT methods have been developed.The study provides key resources and technical platforms for further research as well as anti-viral drug and vaccine development against MPX. | Baoying Huang Hua Zhao Jingdong Song Li Zhao Yao Deng Wen Wang Roujian Lu Wenling Wang Jiao Ren Fei Ye Houwen Tian Guizhen Wu Hua Ling Wenjie Tan | 2022 | China CDC weekly2022，4，46： | 3 |
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