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| 1 | Factors affecting proprioceptive recovery after anterior cruciate ligament reconstruction显示文摘背景 Proprioception 戏在在那里的膝 movements.Since 的一个重要角色是包围 proprioception 追随者外科的全面恢复时间的争吵,在以后,定义影响 proprioceptive 恢复的因素是必要的前面的十字形的系带( ACL )重建并且有他们的 ACL 的 36 个病人的一个总数与 semitendinosus/gracilis 接枝重建了调查在 proprioception 和肌肉 strength.Methods 之间的关系(重建的 group:6 | ZHOU Mou-wang GU Li CHEN Ya-ping YU Chang-long AO Ying-fang HUANG Hong-shi YANG Yan-yan | 2008 | Chinese Medical Journal2008，，22： | 22 |
| 2 | Anatomy of composition and nature of plate convergence: Insights for alternative thoughts for terminal India-Eurasia collision显示文摘The pattern and timing of collision between India and Eurasia have long been a major concern of the international community. However, no consensus has been reached hitherto. To explore and resolve the disagreements in the Himalayan study,in this paper we begin with the methodology and basic principles for the anatomy of composition and nature of convergent margins,then followed by an effort to conduct a similar anatomy for the India-Eurasia collision. One of the most common patterns of plate convergence involves a passive continental margin, an active continental margin and intra-oceanic basins together with accreted terranes in between. The ultimate configuration and location of the terminal suture zone are controlled by the basal surface of the accretionary wedge, which may show fairly complex morphology with Z-shape and fluctuant geometry. One plausible method to determine the terminal suture zone is to dissect the compositions and structures of active continental margins. It requires a focus on various tectonic elements belonging to the upper plate, such as accretionary wedges, high-pressure(HP)-ultra-high-pressure(UHP) metamorphic rocks, Barrovian-type metamorphic rocks and basement nappes, together with superimposed forearc basins.Such geological records can define the extreme limits and the intervening surface separating active margin from the passive one,thus offering a general sketch for the surface trace of the terminal suture zone often with a cryptic feature. Furthermore, the occurrence of the cryptic suture zone in depth may be constrained by geophysical data, which, in combination with outcrop studies of HP-UHP metamorphic rocks, enables us to outline the terminal suture zone. The southern part of the Himalayan orogen records complicated temporal and spatial features, which are hard to be fully explained by the classic 'two-plate-one-ocean' template,therefore re-anatomy of the compositions and nature for this region is necessitated. Taking advantage of the methodology and basic principles of plate convergence anatomy and synthesizing previous studies together with our recent research, we may gain new insights into the evolution of the Himalayan orogeny.(1) The Yarlung-Zangbo ophiolite is composed of multiple tectonic units rather than a single terminal suture zone, and a group of different tectonic units were juxtaposed against each other in the backstop of the Gangdese forearc.(2) The Tethyan Himalayan Sequence(THS) contains mélanges with typical block-in-matrix structures, uniform southwards paleocurrents and age spectra of detrital zircons typical of Eurasia continent. All of these facts indicate that the THS belonged to Eurasia plate before the terminal collision, emplaced in the forearc of the Gangdese arc.(3) The Greater Himalayan Crystalline Complex(GHC) and Lesser Himalayan Sequence(LHS) comprise complex components including eclogites emplaced into the GHC and the upper part of the LHS. Judging from the fact that HP-UHP metamorphic rocks are exhumed and emplaced in the upper plate, the GHC and the upper part of the LHS where eclogite occur should be assigned to the upper plate, lying above the terminal subduction zone surface. It is the very surface along which the continuous subduction of the India subcontinent occurred, therefore acting as the terminal, cryptic suture. From the suture further southward, the bulk rock associations of the LHS and Sub-Himalayan Sequence(Siwalik) show little affinity of mélange, probably belonging to the foreland system of the India plate. By the anatomy of tectonic features of all the tectonic units in the Himalayan orogen as well as the ages of the subduction-accretion related deformation, we conclude that the terminal India-Eurasia collision occurred after 14 Ma, the timing of the metamorphism of the eclogites emplaced into the upper plate. The development of rifts stretching in N-S direction in Tibet and tectonic events with the transition from sinistral to dextral movements in shear zones, such as the Ailaoshan fault in East Tibet, can coordinately reflect the scale and geodynamic influence of the India-Eurasia convergence zone.By conducting a detailed anatomy of the southern Himalayas, we propose a new model for the final collision-accretion of the Himalayan orogeny. Our study indicates that the anatomy of structures, composition, and tectonic nature is the key to a better understanding of orogenic belts, which may apply to all the orogenic belts around the world. We also point out that several important issues regarding the detailed anatomy of the structures, compositions and tectonic nature of the Himalayan orogeny in future. | XIAO WenJiao AO SongJian YANG Lei HAN ChunMing WAN Bo ZHANG Ji'En ZHANG ZhiYong LI Rui CHEN ZhenYu SONG ShuaiHua | 2017 | Science China Earth Sciences2017，60，6： | 20 |
| 3 | Effects of flavonoids from Allium mongolicum Regel on growth performance and growth-related hormones in meat sheep显示文摘This study was conducted to investigate the effects of different doses of flavonoids from Allium mongolicum Regel on the production performance and neuroendocrine hormones in meat sheep and to determine the optimum dosage of Allium mongolicum Regel flavonoids to add to the basal diet of dry lotfeeding meat sheep. Sixty meat sheep(initial body weight = 39.9 ± 3.2 kg; 6-month-old) were randomly assigned to 4 groups(15 sheep per group). The sheep in the control group were fed a basal diet, and the 3 experimental groups were fed the basal diet supplemented with flavonoids at 11.22 and 33 mg/kg. Blood samples were collected via the jugular vein at d 0.15, 30, 45, and 60 to determine the neuroendocrine hormone levels. The fasting weight of the sheep was measured during the experimental period, and feed offered and refusals were recorded daily. The basal diet supplemented with flavonoids from 11 to 33 mg/kg significantly increased the daily weight gain and average daily feed intake(P<0.05) and significantly decreased the feed conversion ratio(P<0.05), but there were no differences among the supplementation groups(P>0.05). Starting on d 30, the growth hormone(GH) and insulin-like growth factor-1(IGF-1)levels in the sera of the sheep in the supplementation groups increased significantly(P<0.05), and the increases occurred in a time-dependent manner. Compared with control group, after d 30, the serum corticosterone(CORT) levels were reduced in the sheep that consumed the basal diet supplemented with22 mg/kg flavonoids(P<0.05), but among the other experimental groups, there was a non-significant effect(P>0.05). The serum adrenocorticotropic hormone(ACTH) levels were increased by the supplementation of flavonoids, but compared with the control group, the effect was not significant. The basal diet supplemented with flavonoids at levels from 11 to 33 mg/kg had a significant effect on the production performance and neuroendocrine hormone levels of meat sheep, and the effect occurred in a time-dependent manner. The effect was especially obvious after 30 d of feeding. | Muqier Sarula Qi Terigele Wang Renwei Chen Cuifang Wang Changjin Ao | 2017 | Animal Nutrition2017，，1： | 18 |
| 4 | A "green pathway" different from simple diffusion in soft matter: Fast molecular transport within micro/nanoscale multiphase porous systems显示文摘 | Jiantao Feng Fang Wang Xinxiao Han Zhuo Ao Quanmei Sun Wenda Hua Peipei Chen Tianwei Jing Hongyi Li Dong Han | 2014 | Nano Research2014，7，3： | 18 |
| 5 | Incidence of infectious complications is associated with a high mortality in patients with hepatitis B virus-related acute-on-chronic liver failure显示文摘BACKGROUND In China,hepatitis B virus-related acute-on-chronic liver failure (HBV-ACLF) is the most common liver failure characterized by serious clinical syndromes of liver decompensation with a very high mortality.Bacterial and/or fungal infections are the most common complications that are associated with high short-term mortality.Bacterial translocation from the intestine,impaired hepatic clearance,and immune paralysis of circulating immune cells are thought to contribute to infectious complications in liver failure.The control of bacterial and fungal infections is the key to improving HBV-ACLF outcomes.Active prevention,early diagnosis,and timely treatment of bacterial and fungal infections are essential for treating HBV-ACLF.AIM To investigate the frequency and role of bacterial and fungal infections in patients with HBV-ACLF.METHODS Patients with HBV-ACLF hospitalized at Taihe Hospital,Hubei University of Medicine from January 2014 to December 2017 were retrospectively enrolled.Patient-related information was retrieved from the hospital case database,including general information,blood biochemistry,complications,etc.According to the occurrence of secondary infection or not,the patients were divided into an infection group and a non-infection group.The sites,types,and incidences of bacterial and fungal infections and the influence of infections on the prognosis of HBV-ACLF were statistically analyzed.The risk factors for infections were assessed by unconditional logistic regression.RESULTS There were 174 cases of HBV-ACLF that met the enrollment criteria,of which 114 (65.52%) were diagnosed with infectious complications.Infections occurred in the abdominal cavity (87 cases),respiratory tract (51 cases),urinary tract (18 cases),and biliary tract (10 cases).Patients with infectious complications had a significantly higher 28-d mortality (70.18%,80/114) than those without (40.00%,24/60)(70.18% vs 40.00%,P < 0.05).And patients with infectious complications had a much higher incidence of non-infectious complications (54.39%,62/114)(54.39% vs 15.00%,P < 0.05),leading to an extremely high 28-d mortality of 88.71%(55/62)(P < 0.05).The grade of liver failure,period of hospital stay ≥ 30 d,age ≥ 45 years,and percentage of neutrophils > 70% were identified as risk factors for infectious complications.CONCLUSION The high incidence of infectious complications in patients with HBV-ACLF is associated with severity and deterioration of the disease and may contribute to the extremely high mortality of these patients. | Chen Wang De-Qiang Ma Sen Luo Chuan-Min Wang De-Ping Ding You-You Tian Kang-Jian Ao Yin-Hua Zhang Yue Chen Zhong-Ji Meng | 2019 | World Journal of Clinical Cases2019，7，16： | 18 |
| 6 | Baicalin protects neonatal rat brains against hypoxicischemic injury by upregulating glutamate transporter 1 via the phosphoinositide 3-kinase/protein kinase B signaling pathway显示文摘Baicalin is a flavonoid compound extracted from Scutellaria baicalensis root.Recent evidence indicates that baicalin is neuroprotective in models of ischemic stroke.Here,we investigate the neuroprotective effect of baicalin in a neonatal rat model of hypoxic-ischemic encephalopathy.Seven-day-old pups underwent left common carotid artery ligation followed by hypoxia(8% oxygen at 37°C) for 2 hours,before being injected with baicalin(120 mg/kg intraperitoneally) and examined 24 hours later.Baicalin effectively reduced cerebral infarct volume and neuronal loss,inhibited apoptosis,and upregulated the expression of p-Akt and glutamate transporter 1.Intracerebroventricular injection of the phosphoinositide 3-kinase/protein kinase B(PI3 K/Akt) inhibitor LY294002 30 minutes before injury blocked the effect of baicalin on p-Akt and glutamate transporter 1,and weakened the associated neuroprotective effect.Our findings provide the first evidence,to our knowledge that baicalin can protect neonatal rat brains against hypoxic-ischemic injury by upregulating glutamate transporter 1 via the PI3 K/Akt signaling pathway. | Zhi-qing Zhou Yong-liang Li Zhen-bo Ao Zhi-li Wen Qi-wen Chen Zheng-gang Huang Bing Xiao Xiao-hua Yan | 2017 | Neural Regeneration Research2017，12，10： | 15 |
| 7 | Studying the mechanical properties of the soil-root interface using the pullout test method显示文摘It is important to quantify the effect of the root diameter, the embedment length of the root and load speed on the soil-root interface mechanical properties for studying the root anchorage. The soilroot interface mechanical properties can be obtained through the pullout force and root slippage curve(F-S curve). About 120 Pinus tabulaeformis single roots whose diameters ranged from 1 mm to 10 mm divided into 6 groups based on different root embedment length(50 mm, 100 mm and 150 mm) and different load velocity(10 mm·min^(-1), 50 mm·min^(-1), 100 mm·min^(-1) and 300 mm·min^(-1)) were investigated using the pullout method. This study aims to explore the mechanical properties of the soil-root interface in the real conditions using the pullout test method. The results showed two kinds of pullout test failure modes during the experimental process: breakage failure and pullout failure. The results showed that the roots were easier to be broken when the root diameter was smaller or the loading speed was larger. The relationship between the maximum anchorage force and root diameter was linear and the linearly dependent coefficient(R^2) was larger than 0.85. The anchorage force increased with the root embedment length. An increase of 10%^(-1)5% for the maximumanchorage force was found when load speed increased from 10 to 300 mm.min^(-1). The mean peak slippage of the root was from 13.81 to 35.79 mm when the load velocity varied from 10 to 300 mm.min^(-1). The study will be helpful for the design of slopes reinforced by vegetation and in predicting risk of uprooting of trees, and will have practical benefits for understanding the mechanism of landslide. | JI Xiao-dong CONG Xu DAI Xian-qing ZHANG Ao CHEN Li-hua | 2018 | Journal of Mountain Science2018，15，4： | 13 |
| 8 | Second-look arthroscopic evaluation of the articular cartilage after primary single-bundle and double-bundle anterior cruciate ligament reconstructions显示文摘在前面的十字形的系带( ACL ) reconstruction.No 报告关于比较变化在以后的软骨被出版了以后,背景几报告显示出关节的软骨退化的前进单个捆( SB )并且这研究的双捆( DB ) ACL reconstructions.The 目的是在 SB 和 DB ACL 以后评估关节的软骨变化由收到了 arthroscopic ACL 重建的 99 个病人回顾地是的秒看 arthroscopy.Methods | WANG Hai-jun AO Ying-fang CHEN Lian-xu GONG Xi WANG Yong-jian MA Yong LEUNG Kevin Kar Ming YU Jia-kuo | 2011 | Chinese Medical Journal2011，，21： | 12 |
| 9 | Effects of multidrug resistance, antisense RNA on the chemosensitivity of hepatocellular carcinoma cells显示文摘BACKGROUND: Multidrug resistance is a major obstacle in cancer chemotherapy. We examined whether the antisense RNA of multidrug resistance gene 1 (mdr1) could reverse multidrug resistance in the human hepatocellular carcinoma (HCC) cell line SMMC7721/ADM. METHODS: The recombinant adenoviruses pAdEasy- GFP-ASmdr1 product was produced by the adenoviral vector AdEasy system, which can express antisense RNA against the mdr1 gene. Following that, the recombinant adenovirus was transfected into the P-glycoprotein- producing multidrug resistance cell line, SMMC7721/ADM human HCC cells resistant to adriamycin (ADM) and daunorubicin (DNR). In order to investigate the reversal of multidrug resistance phenotype, we measured the expression of mdr1 mRNA by RT-PCR and the production of P-glycoprotein by flow cytometry. The sensitivities for ADM and DNR SMMC7721/ADM cells were examined by [3-(4, 5-dimethylthi-azol-2-yl)-2,5 diphenyl-terazolium bromide] (MTT) analysis. RESULTS: The low-level expression of mdr1 mRNA and P-glycoprotein production were observed in parental sensitive cells SMMC/7721 in addition to the overexpressionof mdr1 mRNA and P-glycoprotein in SMMC7721/ADM cells. The transfection of antisense-RNA into SMMC7721/ ADM cells resulted in decreases of mdr1 mRNA and P-glycoprotein, but increase of drug sensitivities. The sensitivities of transfected SMMC7721/ADM cells to ADM and DNR in IC50 reduced by 31.25% and 62.96% respectively. CONCLUSIONS: Mdr1 antisense RNA can increase the sensitivities of SMMC7721/ADM cells to anticancer drug by decreasing the expression of the mdr1 gene and inhibiting P-glycoprotein expression. This strategy may be applicable to cancer patients with P-glycoportein mediated multidrug resistance. | Bo Li, Tian Ye, Lei Zhao, De-Hua Li, Xing-Hua Gou, Lan-Ying Zhao, Lei Han, Lin Chen, Lu-Nan Yan and Jian-Ping Gong Department of Hepatobiliary Surgery, Second Affiliated Hospital, Chongqing University of Medical Sciences, Chongqing 400010, China Department of Traditional Chinese Surgery, Second Affiliated Hospital, Luzhou Medical College, Luzhou 646000, China Genetic Engineering Laboratory, Chengdu Di’ao Group Co., Ltd., Chengdu 610041, China Department of General Surgery, West China Hospital, Sichuan University, Chengdu 610041, China | 2006 | Hepatobiliary & Pancreatic Diseases International2006，5，4： | 10 |
| 10 | HOXC10 up-regulation promotes gastric cancer cell proliferation and metastasis through MAPK pathway显示文摘Objective: As an important regulator of embryonic morphogenesis,homeodomain-containing gene 10(HOXC10) has been found to promote progression of human cancers and its expression indicates poor survival outcome.However,very few studies are available on the role of HOXC10 in gastric carcinoma.Therefore,the aim of this study was to determine the role of HOXC10 in gastric cancer and the potential mechanism underlying its function for cancer biology.Methods: A primary gastric cancer mouse model was obtained via intra-gastric wall injection of gastric cancer cells and was used to evaluate the function of HOXC10 during gastric cancer progression in vivo.Immunohistochemistry was performed to visualize and measure HOXC10 protein expression in gastric cancer tissue.Cells were transfected with plasmids to increase the expression of HOXC10,and si RNA transfection was performed to suppress HOXC10 expression.Reverse transcription polymerase chain reaction(RT-PCR) and western blotting were utilized to measure m RNA and protein expression,respectively.Proliferation,migration,and invasion were investigated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT) assay,wound healing assay,and matrigel invasion assay in vitro,respectively.Results: HOXC10 expression was significantly increased in gastric cancer tissues compared to matched normal tissues.HOXC10 up-regulation significantly increased tumor volumes in nude mice.Plasmid transfection significantly increased HOXC10 protein and m RNA expressions and effectively promoted cell proliferation.Moreover,HOXC10 up-regulation significantly promoted migration and invasion of gastric cancer cells.Mechanistic investigation showed that HOXC10 up-regulation significantly increased m RNA and protein expression of mitogen-activated protein kinase(MAPK) signaling related genes,including c-myc,c-jun and p53,while also modulating the phosphorylation of c-Jun N-terminal kinase(JNK),extracellular signal-regulated kinase(ERK) and P38 but not their total protein levels.Conclusions: This study demonstrated the tight link between HOXC10 and gastric cancer cell proliferation and metastasis via involvement of the MAPK pathway. | Chen Guo Jianing Hou Sheng Ao Xingming Deng Guoqing Lyu | 2017 | Chinese Journal of Cancer Research2017，29，6： | 10 |
| 11 | Joint contractures in severe burn patients with early rehabilitation intervention in one of the largest burn intensive care unit in China:a descriptive analysis显示文摘Background:Joint contracture is the major clinical complication in burn patients,especially,the severe burn patients.This study aimed to investigate the number and severity of joint contractures in patients with burns affecting greater than or equal to 50%of the total body surface area(TBSA)undergoing early rehabilitation in a burn intensive care unit(BICU).Methods:We analyzed burn patients with burns affecting greater than or equal to 50%of the TBSA admitted to a BICU who received early rehabilitation within 7 days post-injury from January 2011 to December 2015.Demographic and medical information was collected.The range of motion(ROM)of different joints was measured 1 month post-admission.Spearman’s correlation coefficient and logistic regression analysis was used to determine predictors of the presence and severity of contractures.Result:The average affected TBSA of the included burn patients was 67.4%,and the average length of stay in the BICU was 46.2±28.8 days.One hundred and one of 108 burn patients(93.5%)developed at least one joint contracture.The ROM in 67.9%of the affected joints was mildly limited.The majority of contractures in severe burn patients were mild(37.7%)or moderate(33.2%).The wrist was the most commonly affected joint(18.2%),followed by the shoulder,ankle,hip,knee,and elbow.A predictor of the presence of contractures was the length of hospital stay(p=0.049).The severe contracture was related to the area of full-thickness burns,the strict bed rest time,and the duration of rehabilitation in BICU.The length of rehabilitation stay(days)in patients with moderate contracture is 54.5%longer than that in severe contracture(p=0.024)Conclusion:During the long stay in BICU,the length of rehabilitation stay in a BICU could decrease the severity of contractures from severe to moderate in the patients with equal to 50%of the TBSA.Hence,this research reveals the important role of early rehabilitation interventions in severe burn patients. | Jianglin Tan Jian Chen Junyi Zhou Huapei Song Huan Deng Ming Ao Gaoxing Luo Jun Wu | 2019 | Burns & Trauma2019，7，1： | 9 |
| 12 | Original article: Arthroscopic treatment for osteochondral lesions of the talus: analysis of outcome predictors显示文摘背景与传统的关节切开术过程相比,为距骨的 osteochondral 损害的 arthroscopic 治疗有一些优点。然而,关于为这种外科的技术的结果预言者有可观的争论。这研究试图为距骨的 osteochondral 损害调查 arthroscopic 治疗的结果,并且分析它的结果 predictors.Methods 经历了脚关节 arthroscopy 的有距骨的 osteochondral 损害的 48 个病人的临床的数据被学习。Arthroscopic 清创术在所有病人上被执行,并且 microfracture 也在 36 种情况中被执行。一张主观满足问询表,为疼痛的视觉模拟规模(管) ,和美国矫形的脚与脚关节社会(AOFAS ) 脚关节上的分数和 hindfoot 分数在五个病人失去了列在后面起来的 surgery.Results 前后被获得。另外的 43 个病人,其中 8 个是运动员,被跟随在上面为 23.9 个月的一般水准。平均 AOFAS 手术后的分数是 90.16 ??????? 贼 ? 贼吗?? | GUO Qin-wei HU Yue-lin JIAO Chen YU Chang-long AO Ying-fang | 2010 | Chinese Medical Journal2010，，3： | 9 |
| 13 | ANALYSIS OF COLUMN-INTEGRATED AEROSOL OPTICAL THICKNESS IN BEIJING FROM AERONET OBSERVATIONS显示文摘Aerosol Optical Thickness (AOT), water vapor content and derived Angstrom exponent acquired by a CIMEL sun photometer in Beijing are analyzed. Monthly means computed from quality-assured daily means, seasonal trends and inter-annual variations are presented and discussed. Summer has the highest seasonal average AOT at 440 nm (τa440), Angstrom exponent (α440–870) and water vapor content with the values 0.93, 1.34 and 3.0 cm, respectively. The second highest seasonal average τa440 appears in spring with the largest variation of α440–870 and minimum α440–870 0.99 due to the impact of coarse particles. The minimum seasonal average τa440 (0.44) and water vapor content (0.4 cm) appear in winter. The annual average τa440, α440–870 and water vapor content for about 4-year observation period are 0.70, 1.19 and 1.4 cm, respectively. All monthly average Angstrom exponents are within 0.8-1.4, which indicates aerosol in Beijing is a very complex mixture of both fine- and coarse-mode particles (from anthropogenic influence and natural mineral dust). | Xuehua Fan Hongbin Chen Philippe Goloub Xiang'ao Xia Wenxing Zhang Bernadette Chatenet | 2006 | China Particuology2006，4，6： | 9 |
| 14 | Clinical evaluation of double-bundle anterior cruciate ligament reconstruction procedure using hamstring tendon grafts: a prospective, randomized and controlled study显示文摘在在那里的临床的研究的背景仍然是很多争吵关于增加了在双捆(DB ) 之间的前面、旋转的稳定性并且单个捆(SB ) 前面的十字形的系带(ACL ) 重建。这研究的目的是评估有到 2006 年 5 月的从 2005 年 5 月的 ACL 破裂的 64 个连续病人随机被分配进二个组的四隧道的 DB ACL reconstruction.Methods 的临床的结果:为 SB ACL 重建的 32 个盒子和为 DB ACL 重建的 32 个盒子。临床的数据,包括的 KT 2000, Biodex 测试, Lysholm 分数, Tegner 分数和 IKDC 得分,有希望地被收集直到一般水准 KT 2000 珍视的至少 10 月 post-operative.Results 是(1. | WANG Jian-quan AO Ying-fang YU Chang-long LIU Ping XU Yan CHEN Lin-xin | 2009 | Chinese Medical Journal2009，，6： | 8 |
| 15 | High glucose causes apoptosis of rabbit corneal epithelial cells involving activation of PERK-eIF2α-CHOP-caspase-12 signaling pathway显示文摘AIM: To investigate the effect of high concentration of glucose(HCG) on double stranded RNA-activated protein kinase-like ER kinase(PERK)-eukaryotic initiation factor-2α(eIF2α)-transcription factor C/EBP homologous protein(CHOP)-cysteine aspartate specific proteinase(caspase-12) signaling pathway activation and apoptosis in rabbit corneal epithelial cells(RCECs). METHODS: RCECs were treated by different concentrations of glucose for 0-48 h. The expressions of PERK, p-PERK, eIF2α, p-eIF2α, 78 k Da glucose-regulated protein 78(GRP78), CHOP, B-cell lymphoma 2(Bcl-2), B-cell lymphoma-2-associated X protein(Bax) and caspase-12 were determined by Western blot. Apoptosis was detected by TUNEL assay. Meanwhile, the function of PERK-eI F2α-CHOP-caspase-12 signaling pathway activation in high glucose-induced apoptosis was evaluated using PERK inhibitor, GSK2606414. RESULTS: HCG significantly promoted the expression of p-PERK, p-eIF2α, GRP78, CHOP, Bax and cleaved caspase-12 in RCECs(P<0.05), while remarkably decreased the expression of Bcl-2 and caspase-12(P<0.05), and the alterations caused by glucose were in concentration-and time-dependent manners. Meanwhile, PERK and eIF2α expressions were not affected in all groups(P>0.05). TUNEL assay showed that the apoptosis rate of RCECs in the HCG group increased significantly in contrast with that in the normal concentration of glucose or osmotic pressure control group(P<0.05), and the apoptosis rate increased with the increase of glucose concentration within limits(P<0.05). GSK2606414 down-regulated the expression of p-PERK and p-eI F2α in the HCG group(P<0.05), while still did not affect the expression of PERK and eIF2α among groups(P>0.05). Correspondingly, GSK2606414 also significantly reduced the apoptosis rate induced by high glucose(P<0.05). CONCLUSION: HCG activates PERK-eIF2α-CHOPcaspase-12 signaling pathway and promotes apoptosis of RCECs. | Pan-Pan Yao Min-Jie Sheng Wen-Hao Weng Yin Long Hao Liu Li Chen Jia-Jun Lu Ao Rong Bing Li | 2019 | International Journal of Ophthalmology(English edition)2019，12，12： | 7 |
| 16 | Dynamic and Thermodynamic Features of Low and Middle Clouds Derived from Atmospheric Radiation Measurement Program Mobile Facility Radiosonde Data at Shouxian, China显示文摘By using the radiosonde measurements collected at Shouxian,China,we examined the dynamics and thermodynamics of single- and two-layer clouds formed at low and middle levels.The analyses indicated that the horizontal wind speed above the cloud layers was higher than those within and below cloud layers.The maximum balloon ascent speed(5.3 m s^(-1)) was located in the vicinity of the layer with the maximum cloud occurrence frequency(24.4%),indicating an upward motion(0.1-0.16 ms^(-1)).The average thickness,magnitude and gradient of the temperature inversion layer above single-layer clouds were117±94 m,1.3±1.3℃ and 1.4±1.5℃(100 m)^(-1),respectively.The average temperature inversion magnitude was the same(1.3℃) for single-low and single-middle clouds;however,a larger gradient[1.7±1.8℃(100 m)^(-1)]and smaller thickness(94±67 m) were detected above single-low clouds relative to those above single-middle clouds[0.9±0.7℃(100 m)^(-1) and157±120 m].For the two-layer cloud,the temperature inversion parameters were 106±59 m,1.0±0.9℃ and 1.0±1.0℃(100 m)^(-1) above the upper-layer cloud and 82 ± 60 m,0.6±0.9℃ and 0.7±0.6℃(100 m)^(-1) above the low-layer cloud.Absolute differences between the cloud-base height(cloud-top height) and the lifting condensation level(equilibrium level)were less than 0.5 km for 66.4%(36.8%) of the cases analyzed in summer. | Jinqiang ZHANG Hongbin CHEN Xiang'ao XIA Wei-Chyung WANG | 2016 | Advances in Atmospheric Sciences2016，33，1： | 7 |
| 17 | Diagnosis of nervous necrosis virus in orange-spotted grouper,Epinephelus coioides, by a rapid and convenient RT-PCR method显示文摘Viral nervous necrosis(VNN)causes high mortality in marine fish,especially in the grouper,worldwide and in China.Since there is no effective vaccine or drug to deal with VNN,early detection and prevention is important to block its outbreak.In this study,a reverse transcription-polymerase chain reaction(RT-PCR)was developed for the rapid,convenient,and sensitive detection of the VNN pathogen,nervous necrosis virus(NNV),in the grouper.The whole process was completed within 3.5 h from the RNA extraction to PCR product visualization.The detection limit of this method was 200 copies of NNV RNA standard,which corresponded to 200 copies of virus particles.This RT-PCR method was specific to the NNV detection with no cross-reactivity to other fish viral disease pathogens,such as infectious pancreatic necrosis virus(IPNV),infectious hematopoietic necrosis virus(IHNV),spring viraemia of carp virus(SVCV),epizootic haematopoietic necrosis virus(EHNV),and large yellow croaker iridovirus(LYCIV).With this method,the orange-spotted grouper(Epinephelus coioides)fry from hatcheries with or without incidence of the VNN epidemic in Fujian Province were detected.The results showed that all or 93%of the fry from the two hatcheries with incidence of the epidemic were diagnosed as positive,while 40%or 25%of fry from the two hatcheries without the VNN epidemic were also detected as NNV positive,indicating that this RT-PCR method can be used for rapid,sensitive detection of NNV infection and applied in the VNN epidemic alert. | MU Yinnan LIN Kebing CHEN Xinhua AO Jingqun | 2013 | Acta Oceanologica Sinica2013，32，10： | 6 |
| 18 | A review:Synthesis,modification and photocatalytic applications of ZnIn_(2)S_(4)显示文摘Solar energy is an ideal energy source for solving energy shortages and serious environmental problems.In the past few decades,photocatalytic technology which uses solar energy to deal with the above problems has caused great interest.ZnIn_(2)S_(4),as a layered ternary metal chalcogenide compound,has a series of advantages such as the wide light absorption range and adjustable bandgap.It has been applied in the different fields of photocatalysis in recent years.This review introduced the crystal structures and growth mechanism of ZnIn_(2)S_(4)and summarized the preparation methods of ZnIn_(2)S_(4).Also,the promoted strategies of ZnIn_(2)S_(4)based photocatalytic system and their applications in the pollutant removal,hydrogen evolution,reduction of CO_(2),nitrogen fixation,and chemical synthesis was summarized.Furthermore,the challenges and development directions of the current ZnIn_(2)S_(4)based photocatalytic system were proposed.It is hoped that this review will help researchers design a better ZnIn_(2)S_(4)based photocatalytic system. | Jie Wang Sijia Sun Run Zhou Yangzi Li Zetian He Hao Ding Daimei Chen Weihua Ao | 2021 | Journal of Materials Science & Technology2021，，19： | 6 |
| 19 | Anchorage properties at the interface between soil and roots with branches显示文摘Roots play an important role in stabilizing and strengthening soil. This article aims to study the mechanical properties of the interface between soil and roots with branches, using the pullout test method in the laboratory.The mechanical properties of the soil–root with branches interface is determined through the pullout-force and rootslippage curve(F–S curve). The results of investigating 24 Pinus tabulaeformis single roots and 55 P. tabulaeformis roots with branches demonstrated three kinds of pullout test failures: breakage failure on branching root, breakage failure on branching node, and pullout failure. The branch angle had a remarkable effect on the failure mode of the roots with branches: the maximum pullout force increased with the sum of the branch diameters and the branch angle.The peak slippage and the initial force had a positive correlation with the sum of the branch diameter. The significance test of correlation between branch angle and the initial force, however, showed they had no correlation.Branch angle and branch root diameter affect theanchorage properties between root system and soil.Therefore, it is important to investigate the anchorage mechanics of the roots with branches to understand the mechanism of root reinforcement and anchorage. | Xiaodong Ji Lihua Chen Ao Zhang | 2017 | Journal of Forestry Research2017，28，1： | 6 |
| 20 | Pathological changes in the lungs and lymphatic organs of 12 COVID-19 autopsy cases显示文摘Systematic autopsy and comprehensive pathological analyses of COVID-19 decedents should provide insights into the disease characteristics and facilitate the development of novel therapeutics.In this study,we report the autopsy findings from the lungs and lymphatic organs of 12 COVID-19 decedents—findings that evaluated histopathological changes;immune cell signature and inflammatory factor expression in the lungs,spleen and lymph nodes.Here we show that the major pulmonary alterations included diffuse alveolar damage,interstitial fibrosis and exudative inflammation featured with extensive serous and fibrin exudates,macrophage infiltration and abundant production of inflammatory factors(IL-6,IP-10,TNFo?and IL-I f).The spleen and hilar lymph nodes contained lesions with tissue structure disruption and immune cell dysregulation,including lymphopenia and macrophage accumulation.These findings provide pathological evidence that links injuries of the lungs and lymphatic organs with the fatal systematic respiratory and immune malfunction in critically ill COVID-19 patients. | Qian Liu Yu Shi Jun Cai Yaqi Duan Rongshuai Wang Hongyan Zhang Qiurong Ruan Jiansha Li Lei Zhao Yifang Ping Rong Chen Liang Ren Xiaochun Fei Heng Zhang Rui Tang Xi Wang Tao Luo Xindong Liu Xuequan Huang Zhenhua Liu Qilin Ao Yong Ren Jing Xiong Zhicheng He Haibo Wu Wenjuan Fu Pengnan Zhao Xinwei Chen Guoqiang Qu Yunyun Wang Xi Wang Jia Liu Dongfang Xiang Sanpeng Xu Xiaowei Zhou Qingrui Li Jinghong Ma Heng Li Jie Zhang Sizhe Huang Xiaohong Yao Yiwu Zhou Chaofu Wang Dingyu Zhang Guoping Wang Liang Liu Xiu-Wu Bian | 2020 | National Science Review2020，7，12： | 6 |
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