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| 1 | Transcriptomics-based identification of WRKY genes and characterization of a salt and hormone-responsive PgWRKY1 gene in Panax ginseng显示文摘WRKY 蛋白质属于一个抄写因素(TF ) 家庭和戏在许多植物过程,对不能生活、关於生命的压力的包括的植物回答,以及第二等的新陈代谢的动态角色。然而,在人参属人参 C ·迈耶的 WRKY 基因都没迄今为止被报导。在这研究,从甲基 jasmonate (MeJA ) 的很多 WRKY unigenes 对待偶然这种的根 transcriptome 用下一代的定序被识别技术。编码 WRKY 蛋白质的 48 有希望的 WRKY unigenes 的一个总数被消除冤屈和不完全的开的读物框架(ORF ) 获得。种系发生的分析揭示 48 WRKY TF,包括 11 组我, 36 组 II,和 1 组 III。而且,指定为 PgWRKY1 的一 MeJA 应答的 unigene 被克隆并且描绘。它包含 1077 bp 的全部 ORF 并且编码 358 氨基酸残余的多肽。PgWRKY1 蛋白质包含由保存氨基酸顺序主题 WRKYGQK 和 C 2 H 2-type 锌手指主题属于 WRKY 亚群 II-d。在洋葱和烟草外皮房间的 PgWRKY1-GFP 熔化蛋白质的 Subcellular 本地化表明 PgWRKY1 在原子核是只在场的。量的即时聚合酶链反应分析证明 PgWRKY1 的表示与叶子,茎,和种子相比在根和侧面的根是相对更高的。重要地, PgWRKY1 表示被水杨酸显著地导致酸, abscisic 酸,和 NaCl,而是由 MeJA 处理的 downregulated。这些结果建议 PgWRKY1 可能是对荷尔蒙和盐压力作出回应的多重压力可诱导的基因。 | Mohammed Nuruzzaman Hongzhe Cao Hao Xiu Tiao Luo Jijia Li Xianghui Chen Junli Luo Zhiyong Luo | 2016 | Acta Biochimica et Biophysica Sinica2016，48，2： | 12 |
| 2 | Nidus vespae protein inhibiting proliferation of HepG2 hepatoma cells through extracellular signal-regulated kinase signaling pathways and inducing G1 cell cycle arrest显示文摘蛋白质说出 NVP (1 ) 从巢 vespae 被孤立。T 他现在的学习的目的是阐明 NVP (1 ) 是否并且怎么调制 HepG2 房间的增长。在 6.6 μg/ml 的集中的 NVP (1 ) 能在阶段 G 1 逮捕房间周期并且禁止 cyclinB, cyclinD1 和 cyclinE 的 mRNA 表示。NVP (1 ) 压制了 cdk2 蛋白质表示,但是增加了 p27 和 p21 蛋白质表示。然而, NVP (1 ) 没改变 p16 蛋白质表示层次。NVP (1 ) 在由原子染色质冷凝作用显示了的 HepG2 房间支持了 apoptosis,并且另外,表明小径的细胞外的调整信号的 kinase (英皇家空军之阶级最低之兵) 被激活。而且,当 HepG2 房间是有英皇家空军之阶级最低之兵禁止者 PD98059 的 pretreated 时,津贴蛋白质表示水平被稀释。这些结果证明 NVP (1 ) 通过表明小径的英皇家空军之阶级最低之兵禁止 HepG2 的增长。NVP (1 ) 能是为肝癌症的潜在的药。 | Changdong Wang Peng Chen Hongjuan Jin Xu Yan Lu Gan Yi Li Shiyi Zhou Junli Chang Yuesheng Wang Guangxiao Yang Guangyuan He | 2008 | Acta Biochimica et Biophysica Sinica2008，40，11： | 11 |
| 3 | The role of RIP3 in cardiomyocyte necrosis induced by mitochondrial damage of myocardial ischemia-reperfusion显示文摘Myocardial damage caused by myocardial ischemia-reperfusion injury (MIRI)is difficult to be alleviated because cardiomyocyte necrosis is an irreversible and unregulated death form.Recently, necroptosis,a necrosis form caused by tumor necrosis factor-α (TNF-α)and Fas ligand (FasL),was found to be regulated by receptor interacting protein 3 (RIP3)and RIP3-receptor interacting protein 1 (RIP1)-mixed lineage kinase domain like protein (MLKL)pathway.But it is unclear whether they also play a regulatory role in MIRI-induced necroptosis.Our previous results showed that in rat MIRI,RIP3 could translocate and express highly in mitochondria.Therefore,it is important to explore proteins that interact with RIP3 which was translocated to mitochondria.The aim of this study was to explore the role of RIP3 in cardiomyocyte necrosis induced by mitochondrial damage of hypoxia/reoxygenation (H/R).Our results showed that H/R could cause RIP3-depended mitochondrial fragmentation and necrosis-based death;and RIP3-promoted H/R-induced necroptosis in H9c2 cells through increasing lactate dehydrogenase release and inhibiting cell viability.This process did not require RIP1 or MLKL but dynamin-related protein 1 (Drp1),which was related to Drpl activation,reactive oxygen species elevation,and △Ψm decline.This study provides novel insights into the role of RIP3 in cardiomyocyte injury during H/R.RIP3 may serve as a potential target for the treatment of MIRI. | Huifang Hou Yongling Wang Qiong Li Zaibing Li Yan Teng Jingyu Li Xiaoying Wang Junli Chen Ning Huang | 2018 | Acta Biochimica et Biophysica Sinica2018，50，11： | 10 |
| 4 | One-step disposal of Cr (Ⅵ)-bearing wastewater by natural pyrrhotite显示文摘Cr(Ⅵ)-bearing wastewater can be treated by natural pyrrhotite which is used for reductant to reduce Cr(Ⅵ) and precipitant to precipitate Cr(Ⅲ) simultaneously. The disposal products can be divided into three parts in the beakers, namely supernatant in the upper part, the yellowish colloidal precipitates in the middle part and the pyrrhotite in the lower part. The content of total Cr=Cr(Ⅵ)+Cr(Ⅲ) in the supernatant liquid is 0.06 mg/L, which is lower than 1.5 mg/L of the discharge standard of China and near to 0.05 mg/L of the standard of potable water. This one-step disposal composing of both reduction and precipitation which is traditionally divided into two independent steps called reducing technology and precipitating technology respectively. The new method is of obvious economic advantage and favourable to decreasing surplus mud derived from adding Ca(OH)2 to precipitate Cr(Ⅲ) traditionally so as to avoid recontamination. In fact, sodium sulfite (Na2SO3) used in disposal of Cr(Ⅵ) was | LU Anhuai, CHEN Jie, SHI Junxian, LU Xiaoying, TANG Junli & GUO Min1. Departmenl of Geology, Peking University, Beijing 100871, China 2. National Laboratory of Mineral and Rock Materials, China University of Geosciences, Beijing 100083, China (e-mail: luanhuai | 2000 | Chinese Science Bulletin2000，45，17： | 9 |
| 5 | Crosstalk Complexities between Auxin, Cytokinin, and Ethylene in Arabidopsis Root Development: From Experiments to Systems Modeling, and Back Again显示文摘理解荷尔蒙和基因怎么交往在变化环境协调植物生长是在植物的主要挑战发展生物学。植物生长素,细胞激动素,和乙烯是调整植物开发的许多方面的三重要荷尔蒙。这评论极其在 Arabidopsis 根开发评估在三荷尔蒙之间的串音。我们把许多试验性的数据集成到一个串音网络,它在植物生长素,细胞激动素,和乙烯串音揭示复杂性的多重层。特别地,数据集成揭示在乙烯和细胞激动素小径之间的一个另外的、大部分俯看的连接,它通过 phosphorelay 机制行动。这个建议连接探讨乙烯申请是否支持或禁止乙烯受体的受体 kinase 活动的突出的问题。阐明在植物生长素,细胞激动素,和乙烯串音的复杂性要求一联合试验性并且系统建模途径。我们为证实在植物生长素,细胞激动素,和乙烯之间的串音怎么在根开发调整 patterning 评估重要当模特儿的努力。我们讨论反复地把实验与为分析建模的系统相结合的新奇方法论怎么为阐明是必要的在在根开发的植物生长素,细胞激动素,和乙烯的串音的复杂性。最后,我们讨论未来挑战从一联合试验性、建模的观点。 | Junli Liu Simon Moore Chunli Chen Keith Lindsey | 2017 | Molecular Plant2017，10，12： | 8 |
| 6 | Spatio-temporal pattern and changes of evapotranspiration in arid Central Asia and Xinjiang of China显示文摘Accurate inversion of land surface evapotranspiration (ET) in arid areas is of great significance for understanding global eco-hydrological process and exploring the spatio-temporal variation and ecological response of water resources.It is also important in the functional evaluation of regional water cycle and water balance,as well as the rational allocation and management of water resources.This study,based on model validation analysis at varied scales in five Central Asian countries and China's Xinjiang,developed an appropriate approach for ET inversion in arid lands.The actual ET during growing seasons of the study area was defined,and the changes in water participating in evaporation in regional water cycle were then educed.The results show the simulation error of SEBS (Surface Energy Balance System) model under cloud amount consideration was 1.34% at 30-m spatial scale,2.75% at 1-km spatial scale and 6.37% at 4-km spatial scale.ET inversion for 1980-2007 applying SEBS model in the study area indicates:(1) the evaporation depth (May-September) by land types descends in the order of waters (660.24 mm) > cultivated land (464.66 mm) > woodland (388.44 mm) > urbanized land (168.16 mm) > grassland (160.48 mm) > unused land (83.08 mm);and (2) ET during the 2005 growing season in Xinjiang and Central Asia was 2,168.68×10 8 m 3 (with an evaporation/precipitation ratio of 1.05) and 9,741.03×10 8 m 3 (with an evaporation/precipitation ratio of 1.4),respectively.The results unveiled the spatio-temporal variation rules of ET process in arid areas,providing a reference for further research on the water cycle and water balance in similar arid regions. | Xi CHEN BaiLian LI Qin LI JunLi LI Saparnov ABDULLA | 2012 | Journal of Arid Land2012，4，1： | 8 |
| 7 | Geographical genetic diversity and divergence of common wild rice (O. rufipogon Griff.) in China显示文摘Using 36 SSR markers and 889 accessions of common wild rice in China, the genetic diversity and the divergence among different geographical populations are investigated. Guangdong Province has the largest number of alleles, which account for 84% of the total alleles detected in the study, followed by Guangxi Province. The Nei's gene diversity indices, from high to low, are in the sequence of Hainan, Guangdong, Guangxi, Fujian, Hunan, Jiangxi, and Yunnan provinces. Two genetic diversity centers of Chinese common wild rice are detected on the basis of geographic analysis, i.e., the region covering Boluo, Zijin, Lufeng, Haifeng, Huidong and Huiyang counties of Guangdong Province and the region covering Yongning, Longan, Laibin and Guigang counties of Guangxi Province. The common wild rice in Yunnan, Hunan, Jiangxi, and Fujian provinces are diverged into respectively independent populations with relatively large genetic distances, whereas, those in Hainan, Guangdong and Guangxi provinces have relatively low genetic divergence. Under the condition of geographic separation, natural selection is considered as one of the primary forces contributing to the divergence of common wild rice in China. | WANG MeiXing ZHANG HongLiang ZHANG DongLing PAN DaJian LI DaoYuan FAN ZhiLan QI YongWen SUN JunLi YANG QingWen LI Chen LI ZiChao | 2008 | Chinese Science Bulletin2008，53，22： | 8 |
| 8 | Screen-imaging guidance using a modified portable video macroscope for middle cerebral artery occlusion显示文摘The use of operating microscopes is limited by the focal length.Surgeons using these instruments cannot simultaneously view and access the surgical field and must choose one or the other.The longer focal length (more than 1 000 mm) of an operating telescope permits a position away from the operating field,above the surgeon and out of the field of view.This gives the telescope an advantage over an operating microscope.We developed a telescopic system using screen-imaging guidance and a modified portable video macroscope constructed from a Computar MLH-10 × macro lens,a DFK-21AU04 USB CCD Camera and a Dell laptop computer as monitor screen.This system was used to establish a middle cerebral artery occlusion model in rats.Results showed that magnification of the modified portable video macroscope was appropriate (5-20 ×) even though the Computar MLH-10 × macro lens was placed 800 mm away from the operating field rather than at the specified working distance of 152.4 mm with a zoom of 1-40 ×.The screen-imaging telescopic technique was clear,life-like,stereoscopic and matched the actual operation.Screen-imaging guidance led to an accurate,smooth,minimally invasive and comparatively easy surgical procedure.Success rate of the model establishment evaluated by neurological function using the modified neurological score system was 74.07%.There was no significant difference in model establishment time,sensorimotor deficit and infarct volume percentage.Our findings indicate that the telescopic lens is effective in the screen surgical operation mode referred to as 'long distance observation and short distance operation' and that screen-imaging guidance using an modified portable video macroscope can be utilized for the establishment of a middle cerebral artery occlusion model and micro-neurosurgery. | Xingbao Zhu Junli Luo Yun Liu Guolong Chen Song Liu Qiangjin Ruan Xunding Deng Dianchun Wang Quanshui Fan Xinghua Pan | 2012 | Neural Regeneration Research2012，7，12： | 6 |
| 9 | Spatio-temporal expression of miRNAs in tomato tissues upon Cucumber mosaic virus and Tomato aspermy virus infections显示文摘MicroRNAs (miRNAs ) 玩在调整的重要角色种生长和开发。最近的工作证明了细胞的 mRNA 表示的那条调停 miRNA 的规定涉及 pathogenhost 相互作用。然而,预定的知识和工厂 miRNA 表示的空间规定仍然是有限的。这里,我们使用茎环确定的即时反向的 transcriptionpolymerase 链反应表示在病原的过程期间在不同西红柿纸巾七 miRNAs 和他们的目标 mRNAs 变化。与接种嘲笑的工厂相比,调查 miRNAs 和 mRNAs 的表示层次被不同的度在黄瓜马赛克之上提高病毒(CMV )-Fny 和西红柿 aspermy virus-Bj 感染,但是在感染的 CMV-Fny2b (CMV-Fny 2b 删除异种) 是几乎未改变的西红柿幼苗。另外,几 miRNAs 的明显的起来规定和在一些西红柿纸巾的目标 mRNAs 在这些纸巾 organogenesis 和发展建议了他们的特殊角色。时间的分析也表明这些 miRNAs 和 mRNAs 的表情被不同病毒的感染高度调整。一起拿,空间地并且时间地观察到在 miRNAs 的变化和目标 mRNAs 表达式层次显示不同病毒的能力防碍 miRNA 小径,并且在不同西红柿纸巾在显型决心与他们的各自的函数被相关。 | Junli Feng Xin Liu Leiyu Lai Jishuang Chen | 2011 | Acta Biochimica et Biophysica Sinica2011，43，4： | 5 |
| 10 | Clinical value of Tongguanteng(Radix seu Herba Marsdeniae Tenacissimae) extract combined with chemotherapy in the treatment of advanced non-small cell lung cancer: a Meta-analysis显示文摘OBJECTIVE: To investigate the clinical efficacy and safety of Tongguanteng(Radix seu Herba Marsdeniae Tenacissimae) extract combined with chemotherapy in the treatment of advanced non-small celllung cancer(NCSLC) compared with chemotherapy alone.METHODS: Databases including Chinese National Knowledge Infrastructure, China Biology Medicine Disc, Wanfang, and MEDLINE were searched until April 1, 2014. Two assessors independently reviewed each trial. The primary outcome was the effective rate(ER) of Tongguanteng(Radix seu Herba Marsdeniae Tenacissimae) extract combined with chemotherapy. The secondary outcomes included quality of life improvement rate(QOLIR) and adverse reactions. Statistical calculations were performed by using Cochrane Collaboration Review Manager 5.2.RESULTS: A total of 888 patients from 15 studies,13 randomized controlled trials(RCT) and two controlled clinical trials, were included. Compared with chemotherapy alone, Tongguanteng(Radix seu Herba Marsdeniae Tenacissimae) extract plus chemotherapy significantly improved ER [Risk ratio(RR) =1.32, 95% CI,(1.14, 1.54)](based on 15 studies) and QOLIR [RR = 2.04, 95% CI,(1.69, 2.47)](based on 13studies). Compared with chemotherapy alone,Tongguanteng(Radix seu Herba Marsdeniae Tenacissimae) extract plus chemotherapy significantly inhibited chemotherapy-induced white blood cell decline [RR = 0.79, 95% CI,(0.70, 0.90)(based on 10 studies), chemotherapy-induced platelet decline[RR = 0.77, 95% CI,(0.60, 0.98)](based on 8 studies),and significantly alleviated nausea and vomiting(NV) [RR = 0.83, 95% CI,(0.71, 0.97)](based on 7studies). There was no significant difference in hemoglobin decline between the two therapies [RR =0.88, 95% CI,(0.70, 1.09)](based on 6 studies).CONCLUSION: This Meta-analysis suggests that Tongguanteng(Radix seu Herba Marsdeniae Tenacissimae) extract combined with chemotherapy may be more efficacious in the treatment of advanced NSCLC than chemotherapy alone. This effect includes enhancing ER and QOLIR, and weakening chemotherapy toxicity. However, large-scale RCTs are required to further investigate the short- and long-term effects of Tongguanteng(Radix seu Herba Marsdeniae Tenacissimae) extract. | Zhang Haiming Zhang Junli Ding Hao Chen Rui Liang Fengxia | 2016 | Journal of Traditional Chinese Medicine2016，36，3： | 4 |
| 11 | Effect of Nitrogen Fertilizer on the Accumulation and Distribution of Dry Matter in Broomcorn Millet显示文摘[Objective] To understand the effect of nitrogen application on dry matter accumulation and allocation dynamics in broomcorn millet. [Method] The accumulation and distribution of dry matter were studied using cultivars Jin Shu 7 and Huang Mizi at different levels of nitrogen fertilizer at the jointing stage. [Result] The results showed that increasing N application led to the increase of green leaf area and the delay of leaf senescence, which was beneficial to the accumulation of dry matter.Appropriate nitrogen application(90 kg/hm2) could coordinate the translocation rate of dry matter among different plant parts, thereby enhancing the yield of broomcorn millet; among different organs, the contribution rate of stem to kernel was greater than that of leaf to kernel; there was obvious correlation between dry matter and yield. For Jin Shu 7, leaf area and dry weight of spike showed significant negative correlation with yield. [Conclusion] The formation of grain yield of broomcorn millet involved the accumulation and allocation of dry matter, the appropriate amount of nitrogen application(90 kg/hm2) could improve the rates of translocation and contribution of dry matter, thereby promoting the yield of broomcorn millet. | Ling CHEN Zhijun QIAO Junjie WANG Haigang WANG Xiaoning CAO Junli DONG | 2015 | Agricultural Science & Technology2015，16，7： | 4 |
| 12 | Severity of early allograft dysfunction following donation after circulatory death liver transplantation:a multicentre study显示文摘Background:Early allograft dysfunction(EAD)is associated with decreased graft and patient survival rates.This study aimed to identify the severity of EAD and develop a predictive model for EAD after donation after circulatory death(DCD)liver transplantation(LT).Furthermore,the influence of operative time on EAD incidence was also evaluated.Methods:In this retrospective,multicentre cohort study,nomograms were established based on a single-centre training cohort(n=321)and validated in a 3-center validation cohort(n=501).Results:The incidence rate of EAD was 46.4%(149/321)in the training cohort and 40.5%(203/501)in the validation cohort.Of the 149 EAD patients in the training cohort,77 patients with either elevated alanine aminotransferase(ALT)or aspartate aminotransferase(AST)were classified as having EAD type A,and the rest of the EAD patients were classified as having EAD type B.Recipients with EAD type B had lower graft and patient survival rates than recipients with EAD type A(P=0.043 and 0.044,respectively).We further developed a nomogram to predict EAD(graft weight,cold ischemia time,donor age,model for end-stage liver disease(MELD)score)and another nomogram to predict EAD type B(graft weight,cold ischemia time,MELD score).The nomograms for the prediction of EAD and EAD type B had good discrimination[concordance index(C-index)=0.712(0.666-0.758),0.707(0.641-0.773)]and calibration[Hosmer-Lemeshow(HL)P=0.384,P=0.425]in the validation cohort.An increased operative time(>6 h)was associated with increased EAD and EAD type B incidence in the high-risk group(P=0.005,P=0.020,respectively).Conclusions:EAD type B was associated with decreased graft and patient survival rates.The novel nomograms effectively predicted the incidence of EAD and EAD type B in DCD LT patients. | Kun Wang Di Lu Yuhui Liu Wangyao Li Li Zhuang Zhenyu Ma Qinfen Xie Binhua Pan Yichao Wu Junli Chen Lidan Lin Xiaowen Feng Qiang Wei Xuyong Wei Haiyang Xie Zhengxin Wang Shusen Zheng Xiao Xu | 2021 | Hepatobiliary Surgery and Nutrition2021，10，1： | 4 |
| 13 | Top-k probabilistic prevalent co-location mining in spatially uncertain data sets显示文摘 | Lizhen WANG Jun HAN Hongmei CHEN Junli LU | 2016 | Frontiers of Computer Science2016，10，3： | 4 |
| 14 | Activation of anterior thalamic reticular nucleus GABAergic neurons promotes arousal from propofol anesthesia in mice显示文摘Propofol is widely used for the induction and maintenance of anesthesia,which causes a rapid loss of consciousness.However,the mechanisms underlying the hypnosis effect of propofol are still not fully understood.The thalamic reticular nucleus(TRN)is crucial for regulating wakefulness,sleep rhythm generation,and sleep stability,while the role of TRN in the process of propofol-induced anesthesia is still unknown.Here,we investigated the function of the anterior TRN in propofol general anesthesia.Our results demonstrated that the neural activity of anterior TRN is suppressed during propofol anesthesia,whereas it is robustly activated from anesthesia by recording the calcium signals using fiber photometry technology.The results showed that the activation of anterior TRN neurons by chemogenetic and optogenetic methods shortens the emergency time without changing the induction time.Conversely,chemogenetic or optogenetic inhibition of the TRN neurons leads to a delay in the recovery time.Our study showed that anterior TRN is crucial for behavioral arousal without affecting the induction time of propofol anesthesia. | Yanjun Liu Bing Chen Yirong Cai Yuan Han Ying Xia Nanqi Li Bingqian Fan Tianjie Yuan Junli Jiang Po Gao Weifeng Yu Yingfu Jiao Wenxian Li | 2021 | Acta Biochimica et Biophysica Sinica2021，53，7： | 3 |
| 15 | Trp_(548)Met mutation of acetolactate synthase in rice confers resistance to a broad spectrum of ALS-inhibiting herbicides显示文摘Herbicide resistance in crop plants is valuable for integrated weed management in agriculture. Herbicide resistant rice, in particular, is important to management of weedy rice, a close relative of cultivated rice and a noxious weed prevalent in rice fields that remains challenging to farmers worldwide. Herbicide resistant plants can be obtained through transgenic approach or by mutagenesis of regular plant and screening of mutants with elevated resistance to herbicide. In this study, we conducted ethyl methyl sulfonate mutagenesis(EMS) to elite indica cultivar Huanghuazhan(HHZ) and screened for mutants resistant to imazapic, a herbicide that can inhibit the acetolactate synthase(ALS) in plants. We obtained three mutants of Os ALS gene that have not been reported previously in rice. One of the mutants, with Trp_(548) changed to Met(W_(548)M), was analyzed in more details in this study. This mutation had no negative effect on the plant physiology and morphology as well as rice yield. Compared with the imidazolinone-resistant mutant S_(627)N(Ser_(627) changed to Asn) that has been deployed for Clearfield rice development, W_(548)M mutant showed high levels of resistance to a broad spectrum of five families of ALSinhibiting herbicides, in addition to a higher level of resistance to herbicides of the imidazolinone family.The herbicide-resistance was stably inherited by crossing into other rice lines. Thus, the W_(548)M mutation provides a valuable resource for breeding of herbicide resistant rice and weed management. | Lei Chen Gang Gu Chengxu Wang Zhufeng Chen Wei Yan Man Jin Gang Xie Junli Zhou Xing Wang Deng Xiaoyan Tang | 2021 | The Crop Journal2021，9，4： | 3 |
| 16 | Time Sequence of Himalayan Endogenetic Mineralization on the East Side of the Qinghai-Tibet Plateau显示文摘Among the endogenetic deposits in the Sanjiang area and at the west margin of the Yangtze platform, Himalayan deposits are the most important and contribute a large proportion of the resources of superlarge deposits. Among the controlled resources of this region, 84% of copper resources, 67% of Pb-Zn, 31% of Ag, 77% of gold and 24% of tin come from Himalayan deposits on the east side of the Qinghai-Tibet plateau. Himalayan endogenetic mineralization shows a relatively complete sequence evolution in the Sanjiang area and on the west margin of the Yangtze platform. Mineralization is manifested by gold deposits related to K-rich lamprophyre, REE deposits related to alkalic complexes and Cu-Au-polymetallic deposits related to alkaline porphyry. Six sequences of mineralization evolution since 65 Ma B.P. in the Sanjiang area and on the west side of the Yangtze platform can be recognized. Himalayan endogenetic mineralization on the east side of the Qinghai-Tibet plateau reached its peak before the Oligo-cene, | LUO Junlie CHEN Yuchuan WANG Denghong | 2000 | Acta Geologica Sinica(English Edition)2000，74，3： | 2 |
| 17 | Satellite RNA-mediated Reduction of Cucumber Mosaic Virus Genomic RNAs Accumulation in Nicotiana tabacum显示文摘卫星 RNA (satRNAs ) 是防碍助手病毒的致病的分子的寄生虫。在这研究,黄瓜马赛克的相对累积病毒(CMV )-Fny genomic RNA 与或没有 satRNAs 是 real-timeRT-PCR 分析的份量上。结果证明 satRs 显然由压抑 CMV-Fny genomic RNA 的累积在 Nicotianatabacum 上稀释了 CMV-Fny 的症状,测试同样开的读物框架。CMV-Fny 1a, 2a, 2b, 3a,和 CP 基因的 Theaccumulation 比有 satRsadded (CMV-Fsat ) 的 CMV-Fny 的高得多,在不同接种时间。 CMV-Fny Δ2 b ,在 2a 基因的C终端的完全的 2b 基因和 41amino 酸在被删除,在 N.tabacum 幼苗上引起了仅仅细微马赛克效果,类似于 CMV-Fsat 的,但是到 CMV-Fny Δ2 b 的 satRs 的增加在 CMV-Fny Δ2 b genomic RNA 的累积显示出进一步的减少。我们的结果显示由增加 satRs 或删除 2b 基因, CMV 的变细由于 CMVgenomic RNA,和在 N 的 CMV genomic RNA 累积的那调停 satRNA 的减小的低累积。tabacum 是可能与 2b 基因有关。 | Qiansheng LIAO Liping ZHU Zhiyou DU Rong ZENG Junli FENG Jishuang CHEN | 2007 | Acta Biochimica et Biophysica Sinica2007，39，3： | 2 |
| 18 | AP-2α expression and cell apoptosis of the lung tissue of rats with COPD and ECV304 cells stimulated by cigarette smoke extract显示文摘An increasing body of evidence suggests that apoptosis of structural cells in the lung might be an important upstream event in the pathogenesis of chronic obstructive pulmonary disease (COPD).AP-2α is one of the important transcription factors involved in the modulation of apoptosis in carcinogenesis and idiopathic-dilated cardiomyopathy.The relationship between AP-2α and apoptosis in COPD remains to be elucidated.The aim of the present study was to investigate the expression of AP-2α in the lung tissues of rats with COPD induced by smoking and its possible protective effect on cigarette smoke extract (CSE) induced endothelial cell apoptosis.Sprague-Dawley rats (n=24) were randomly assigned to normal and COPD groups.The COPD group was exposed to smoke from 20 commercial unfiltered cigarettes for 80 d before morphological assessment of the lung tissue was performed.The expression of AP-2α in lung tissues was measured by Western blotting.To demonstrate the relationship between apoptosis and AP-2α,in vitro cell experiments were carried out.Cells were treated with different concentrations of CSE before proliferation was measured by MTT.Apoptosis was then determined by Hoechst staining and the expression of cleaved caspase-3 and AP-2α by Western blotting over time following treatment with 5% CSE.Cells were then infected with an AP-2α adenovirus vector and the expression of cleaved caspase-3 and AP-2α was compared to the control groups by Western blotting.The COPD group showed larger air spaces and significant decrease of FEV 0.3/FVC compared with the rats in the control group (P<0.05).The expression of AP-2α was significantly higher in the lung tissue of rats with COPD compared with those of controls (P<0.05).In the ECV304 cells,CSE induced apoptosis (P<0.01) and caspase-3 activation in a time-dependent manner and reduced the cell proliferation rate in a dose-dependent manner (P<0.005).Moreover,5% CSE treatment increased endogenous AP-2α protein expression.AP-2α overexpression inhibited 5% CSE-induced cell apoptosis and activated caspase-3 expression (P<0.05) AP-2α protects ECV304 cells against CSE-induced apoptosis and may play an important role in smoking induced-apoptosis in COPD. | LI JunLi CHEN Yan CHEN Ping CAI Shan PENG Hong ZHOU Rui XIANG XuDong LONG Hong LIU ShaoKun | 2011 | Chinese Science Bulletin2011，56，15： | 2 |
| 19 | Down regulated expression of S_(2)-RNase attenuates self-incompatibility in “Guiyou No.1”pummelo显示文摘Self-incompatibility(SI)substantially restricts the yield and quality of citrus.Therefore,breeding and analyzing selfcompatible germplasm is of great theoretical and practical signi ficance for citrus.Here,we focus on the mechanism of a self-compatibility mutation in‘Guiyou No.1'pummelo(Citrus maxima),which is a spontaneous mutant of‘Shatian’pummelo(Citrus maxima,self-incompatibility).The rate of fruit set and the growth of pollen tubes in the pistil con firmed that a spontaneous mutation in the pistil is responsible for the self-compatibility of‘Guiyou No.1'.Segregation ratios of the S genotype in progeny,expression analysis,and western blotting validated that the reduced levels of S_(2)-RNase mRNA contribute to the loss of SI in‘Guiyou No.1'.Furthermore,we report a phased assembly of the‘Guiyou No.1'pummelo genome and obtained two complete and well-annotated S haplotypes.Coupled with an analysis of SV variations,methylation levels,and gene expression,we identi fied a candidate gene(CgHB40),that may in fluence the regulation of the S/^RNase promoter.Our data provide evidence that a mutation that affects the pistilled to the loss of SI in‘Guiyou No.1'by in fluencing a poorly understood mechanism that affects transcriptional regulation.This work signi ficantly advances our understanding of the genetic basis of the SI system in citrus and provides information on the regulation of S-RNase genes. | ianbing Hu Qiang Xu Chenchen Liu Binghao Liu Chongling Deng Chuanwu Chen Zhuangmin Wei Muhammad Husnain Ahmad Kang Peng Hao Wen Xiangling Chen Peng Chen Robert MLarkin Junli Ye Xiuxin Deng Lijun Chai | 2021 | Horticulture Research2021，8，1： | 2 |
| 20 | Controlling Factors on Organic Matter Accumulation of Marine Shale across the Ordovician–Silurian Transition in South China: Constraints from Trace-Element Geochemistry显示文摘In recent years,significant progress in shale gas exploration has been achieved in the Upper Ordovician(Wufeng Formation)–Lower Silurian(Longmaxi Formation)shales in the Upper Yangtze area,South China.Although many studies have been carried out on the Upper Ordovician–Lower Silurian shales,the controlling factors causing organic matter accumulation of these shales remain controversial.This study uses trace-element geochemistry and sedimentological methods to evaluate terrigenous input,redox conditions and primary productivity to explore the mechanisms of organic matter accumulation.The variation of terrigenous fraction elements(Al,Th and Sc)concentrations reflect a mixed influence of sea-level change and weathering.The sea-level of the Upper Yangtze Sea went through two cycles of transgression to regression during the Ordovician–Silurian transition.The Linxiang Formation,Kuanyinchiao Bed and the upper part of Longmaxi Formation developed during the periods of regression,whereas the Wufeng Formation and the lower part of the Longmaxi Formation developed during the periods of transgression.The paleo-productivity indexes of TOC content,ratios of Ba/Al and P/Al,and redox conditions proxies of Mo concentration,ratios of U/Th and V/Cr generally display similar variation patterns with respect to the sea-level changes.High TOC contents and Ba/Al and P/Al ratios indicate the paleo-productivity was high on the sea surface,as shown by relatively good positive correlations between Th vs.TOC,and Sc vs.TOC.This indicates that the paleo-productivity was controlled by the nutrients input through weathering.The good positive correlations between redox conditions indexes(U/Th and V/Cr ratios)with TOC content reflects reductive preservation conditions(anoxic to euxinic),thus implying they were an important controlling factor for organic matter accumulation.Nevertheless,redox conditions were closely associated with sea level change and organic matter decomposition.Therefore,the sea-level change and weathering were the primary controlling factors for organic matter enrichment across the Ordovician to Silurian transition. | Zhen Qiu Huifei Tao Bin Lu Zhenhong Chen Songtao Wu Hanlin Liu Junli Qiu | 2021 | Journal of Earth Science2021，32，4： | 2 |
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