|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | The Chinese Society of Clinical Oncology(CSCO):Clinical guidelines for the diagnosis and treatment of gastric cancer,2021显示文摘There exist differences in the epidemiological characteristics,clinicopathological features,tumor biological characteristics,treatment patterns,and drug selections between gastric cancer patients from the Eastern and Western countries.The Chinese Society of Clinical Oncology(CSCO)has organized a panel of senior experts specializing in all sub-specialties of gastric cancer to compile a clinical guideline for the diagnosis and treatment of gastric cancer since 2016 and renews it annually.Taking into account regional differences,giving full consideration to the accessibility of diagnosis and treatment resources,these experts have conducted expert consensus judgment on relevant evidence and made various grades of recommendations for the clinical diagnosis and treatment of gastric cancer to reflect the value of cancer treatment and meeting health economic indexes in China.The 2021 CSCO Clinical Practice Guidelines for Gastric Cancer covers the diagnosis,treatment,follow-up,and screening of gastric cancer.Based on the 2020 version of the CSCO Chinese Gastric Cancer guidelines,this updated guideline integrates the results ofmajor clinical studies from China and overseas for the past year,focused on the inclusion of research data from the Chinese population for more personalized and clinically relevant recommendations.For the comprehensive treatment of non-metastatic gastric cancer,attentions were paid to neoadjuvant treatment.The value of perioperative chemotherapy is gradually becoming clearer and its recommendation level has been updated.For the comprehensive treatment of metastatic gastric cancer,recommendations for immunotherapy were included,and immune checkpoint inhibitors fromthird-line to the first-line of treatment for different patient groups with detailed notes are provided. | Feng-Hua Wang Xiao-Tian Zhang Yuan-Fang Li Lei Tang Xiu-Juan Qu Jie-Er Ying Jun Zhang Ling-Yu Sun Rong-Bo Lin Hong Qiu Chang Wang Miao-Zhen Qiu Mu-Yan Cai QiWu Hao Liu Wen-Long Guan Ai-Ping Zhou Yu-Jing Zhang Tian-Shu Liu Feng Bi Xiang-Lin Yuan Sheng-Xiang Rao Yan Xin Wei-Qi Sheng Hui-Mian Xu Guo-Xin Li Jia-Fu Ji Zhi-Wei Zhou Han Liang Yan-Qiao Zhang Jing Jin Lin Shen Jin Li Rui-Hua Xu | 2021 | Cancer Communications2021，41，8： | 97 |
| 2 | A complete sequence and comparative analysis of a SARS-associated virus(Isolate BJ01)显示文摘The genome sequence of the Severe Acute Respiratory Syndrome (SARS)-associated virus provides essential information for the identification of pathogen(s), exploration of etiology and evolution, interpretation of transmission and pathogenesis, development of diagnostics, prevention by future vaccination, and treatment by developing new drugs. We report the complete genome sequence and comparative analysis of an isolate (BJ01) of the coronavirus that has been recognized as a pathogen for SARS. The genome is 29725 nt in size and has 11 ORFs (Open Reading Frames). It is composed of a stable region encoding an RNA-dependent RNA polymerase (composed of 2 ORFs) and a variable region representing 4 CDSs (coding sequences) for viral structural genes (the S, E, M, N proteins) and 5 PUPs (putative uncharacterized proteins). Its gene order is identical to that of other known coronaviruses. The sequence alignment with all known RNA viruses places this virus as a member in the family of Coronaviridae. Thirty putative substitutions have been identified by comparative analysis of the 5 SARS- associated virus genome sequences in GenBank. Fifteen of them lead to possible amino acid changes (non-synonymous mutations) in the proteins. Three amino acid changes, with predicted alteration of physical and chemical features, have been detected in the S protein that is postulated to beinvolved in the immunoreactions between the virus and its host. Two amino acid changes have been detected in the Mprotein, which could be related to viral envelope formation. Phylogenetic analysis suggests the possibility of non-human origin of the SARS-associated viruses but provides noevidence that they are man-made. Further efforts should focus on identifying the etiology of the SARS-associated virus and ruling out conclusively the existence of otherpossible SARS-related pathogen(s). | QIN E'de ZHU Qingyu YU Man FAN Baochang CHANG Guohui SI Bingyin YANG Bao PENG Wenming JIANG Tao LIU Bohua DENG Yongqiang LIU Hong ZHANG Yu WANG Cui LI Yuquan GAN Yonghua LI Xiaoyu L Fushuang TAN Gang CAO Wuchun, YANG Ruifu Institute of Microbiology and Epidemiology, Chinese Academy of Military Medical Sciences, Beijing 100071, China WANG Jian, LI Wei, XU Zuyuan, LI Yan, WU Qingfa, LIN Wei, CHEN Weijun, TANG Lin, DENG Yajun, HAN Yujun, LI Changfeng, LEI Meng, LI Guoqing, LI Wenjie, L Hong, SHI Jianping, TONG Zongzhong, ZHANG Feng, LI Songgang, LIU Bin, LIU Siqi, DONG Wei, WANG Jun, Gane K-S Wong, YU Jun & YANG Huanming* Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300 National Center for Genome Information, Beijing 101300, China | 2003 | Chinese Science Bulletin2003，48，10： | 121 |
| 3 | Mesenchymal stem cells derived from human placenta suppress allogeneic umbilical cord blood lymphocyte proliferation显示文摘ABSTRACTHuman placenta-derived mononuclear cells (MNC) were isolated by a Percoll density gradient and cultured in mesen-chymal stem cell (MSC) maintenance medium. The homogenous layer of adherent cells exhibited a typical fibroblast-like morphology, a large expansive potential, and cell cycle characteristics including a subset of quiescent cells. In vitrodifferentiation assays showed the tripotential differentiation capacity of these cells toward adipogenic, osteogenic andchondrogenic lineages. Flow cytometry analyses and immunocytochemistry stain showed that placental MSC was ahomogeneous cell population devoid of hematopoietic cells, which uniformly expressed CD29, CD44, CD73, CD105,CD166, laminin, fibronectin and vimentin while being negative for expression of CD31, CD34, CD45 and α-smoothmuscle actin. Most importantly, immuno-phenotypic analyses demonstrated that these cells expressed class I majorhistocompatibility complex (MHC-I), but they did not express MHC-II molecules. Additionally these cells could sup-press umbilical cord blood (UCB) lymphocytes proliferation induced by cellular or nonspecific mitogenic stimuli. Thisstrongly implies that they may have potential application in allograft transplantation. Since placenta and UCB arehomogeneous, the MSC derived from human placenta can be transplanted combined with hematopoietic stem cells(HSC) from UCB to reduce the potential graft -versus-host disease (GVHD) in recipients. | Chang Dong LI Wei Yuan ZHANG He Lian LI Xiao Xia JIANG Yi ZHANG Pei Hsien TANG Ning MAO | 2005 | Cell Research2005，15，7： | 36 |
| 4 | Comparison of bupivacaine, ropivacaine and levobupivacaine with sufentanil for patient-controlled epidural analgesia during labor: a randomized clinical trial显示文摘背景 Ropivacaine 和 levobupivacaine 与与 bupivacaine 相比引起更少的马达块和毒性的建议优点被介绍了进产科的止痛实践。然而,麻药是否两个都为劳动痛觉缺失相对 bupivacaine 与任何临床的利益被联系,仍然是争论的。这研究试图为控制病人的硬膜上的劳动痛觉缺失在更低的集中比较止痛功效,马达块和 bupivacaine, ropivacaine 和 levobupivacaine 的副作用。450 从未生育过者的 parturients 在这使随机化的临床的试用被注册的方法。0.05% 任何一个 bupivacaine, 0.075% , 0.1% , 0.125% 或 0.15%( 组 B ) 的集中, ropivacaine (组 R ) 或有 sufentanil 的 levobupivacaine (组 L ) 0.5 g/ml 被控制病人的痛觉缺失硬膜上地管理模式。因为一个视觉类似物规模分数是 30 公里,有效痛觉缺失被定义。为每局部麻醉的相对中部的力量用一个概率单位回归模型被计算。Parturients 人口分布,感觉并且马达封锁,产科的数据,母亲的副作用,时时,使用的局部麻醉的体积,和其它也被注意。在那里的结果不是在在有效痛觉缺失的数字的组之中的重要差别,疼痛分数,时时,局部麻醉数量使用了,感觉并且马达封锁,劳动持续时间和交货,副作用和母亲的满足的模式(P > 0.05 ) 。相对中部的力量是 bupivacaine/ropivacaine:0.828 (0.602-1.091 ) , bupivacaine/levobupivacaine:0.845 (0.617-1.12 ) , ropivacaine/levobupivacaine:1.021 (0.774-1.354 ) 分别地。然而,一少些显著地时时有效痛觉缺失并且更高数局部麻醉使用在每个组以内 0.1% 比那些在 0.05% 的集中被观察(P < 0.05 ) 。用有 sufentanil 的控制病人的硬膜上的痛觉缺失, bupivacaine 的更低的集中, ropivacaine 和 levobupivacaine 的结论为劳动痛觉缺失生产类似的痛觉缺失和马达块和安全。止痛功效主要取决于集中而非麻药的类型。 | WANG Li-zhong CHANG Xiang-yang LIU Xia HU Xiao-xia TANG Bei-lei | 2010 | Chinese Medical Journal2010，，2： | 31 |
| 5 | Terrestrial carbon sinks in China and around the world and their contribution to carbon neutrality显示文摘Enhancing the terrestrial ecosystem carbon sink(referred to as terrestrial C sink) is an important way to slow down the continuous increase in atmospheric carbon dioxide(CO_(2)) concentration and to achieve carbon neutrality target.To better understand the characteristics of terrestrial C sinks and their contribution to carbon neutrality,this review summarizes major progress in terrestrial C budget researches during the past decades,clarifies spatial patterns and drivers of terrestrial C sources and sinks in China and around the world,and examines the role of terrestrial C sinks in achieving carbon neutrality target.According to recent studies,the global terrestrial C sink has been increasing from a source of (-0.2±0.9) Pg C yr^(-1)(1 Pg=1015g)in the 1960s to a sink of (1.9±1.1) Pg C yr^(-1) in the 2010s.By synthesizing the published data,we estimate terrestrial C sink of 0.20–0.25 Pg C yr^(-1) in China during the past decades,and predict it to be 0.15–0.52 Pg C yr^(-1) by 2060.The terrestrial C sinks are mainly located in the mid-and high latitudes of the Northern Hemisphere,while tropical regions act as a weak C sink or source.The C balance differs much among ecosystem types:forest is the major C sink;shrubland,wetland and farmland soil act as C sinks;and whether the grassland functions as C sink or source remains unclear.Desert might be a C sink,but the magnitude and the associated mechanisms are still controversial.Elevated atmospheric CO_(2) concentration,nitrogen deposition,climate change,and land cover change are the main drivers of terrestrial C sinks,while other factors such as fires and aerosols would also affect ecosystem C balance.The driving factors of terrestrial C sink differ among regions.Elevated CO_(2) concentration and climate change are major drivers of the C sinks in North America and Europe,while afforestation and ecological restoration are additionally important forcing factors of terrestrial C sinks in China.For future studies,we recommend the necessity for intensive and long-term ecosystem C monitoring over broad geographic scale to improve terrestrial biosphere models for accurately evaluating terrestrial C budget and its dynamics under various climate change and policy scenarios. | Yuanhe Yang Yue Shi Wenjuan Sun Jinfeng Chang Jianxiao Zhu Leiyi Chen Xin Wang Yanpei Guo Hongtu Zhang Lingfei Yu Shuqing Zhao Kang Xu Jiangling Zhu Haihua Shen Yuanyuan Wang Yunfeng Peng Xia Zhao Xiangping Wang Huifeng Hu Shiping Chen Mei Huang Xuefa Wen Shaopeng Wang Biao Zhu Shuli Niu Zhiyao Tang Lingli Liu Jingyun Fang | 2022 | Science China(Life Sciences)2022，65，5： | 32 |
| 6 | Rock burst mechanism analysis in an advanced segment of gob-side entry under different dip angles of the seam and prevention technology显示文摘In order to investigate the frequent occurrences of rock burst in gob-side entry during the mining process of the mining zone No. 7, the mechanical model of main roof of fully-mechanized caving mining before breaking was established by the Winkler foundation beam theory, and the stress evolution law of surrounding rock with different dip angles of the seam during the mining process was analyzed by using FLAC3 D. The results show that: with the dip angle changing from 45° to 0°, the solid-coal side of gobside entry begins to form an L-shaped stress concentration zone at a dip angle of 30°, and the stress concentration degree goes to higher and higher levels. However, the stress concentration degree of the coalpillar side goes to lower and lower levels; the influence range and peak stress of the abutment at the lateral strata of adjacent gob increase with dip angle decreasing and reach a maximum value at a dip angle of 0°, but the tailgate is not affected; the abutment pressure superposition of two adjacent gobs leads to stress concentration further enhancing in both sides of gob-side entry. With the influence of strong mining disturbance, rock burst is easily induced by dynamic and static combined load in the advanced segment of gob-side entry. To achieve stability control similar to that in the roadway, the key control strategy is to reinforce surrounding rock and unload both sides. Accordingly, the large-diameter drilling and high-pressure water injection combined unloading and reinforced support cooperative control technology was proposed and applied in field test. The results of Electromagnetic Emission(EME) and field observation showed that unloading and surrounding rock control effect was obvious. | Yang Zengqiang Liu Chang Tang Shichuan Dou Linming Cao Jinglong | 2018 | International Journal of Mining Science and Technology2018，28，6： | 24 |
| 7 | BMAL1 knockout macaque monkeys display reduced sleep and psychiatric disorders显示文摘Circadian disruption is a risk factor for metabolic, psychiatric and age-related disorders, and non-human primate models could help to develop therapeutic treatments. Here, we report the generation of BMAL1 knockout cynomolgus monkeys for circadian-related disorders by CRISPR/Cas9 editing of monkey embryos. These monkeys showed higher nocturnal locomotion and reduced sleep, which was further exacerbated by a constant light regimen. Physiological circadian disruption was reflected by the markedly dampened and arrhythmic blood hormonal levels. Furthermore, BMAL1-deficient monkeys exhibited anxiety and depression, consistent with their stably elevated blood cortisol, and defective sensory processing in auditory oddball tests found in schizophrenia patients. Ablation of BMAL1 up-regulated transcriptional programs toward inflammatory and stress responses, with transcription networks associated with human sleep deprivation, major depressive disorders, and aging. Thus, BMAL1 knockout monkeys are potentially useful for studying the physiological consequences of circadian disturbance, and for developing therapies for circadian and psychiatric disorders. | Peiyuan Qiu Jian Jiang Zhen Liu Yijun Cai Tao Huang Yan Wang Qiming Liu Yanhong Nie Fang Liu Jiumu Cheng Qing Li Yun-Chi Tang Mu-ming Poo Qiang Sun Hung-Chun Chang | 2019 | National Science Review2019，6，1： | 21 |
| 8 | Computing Power Network:The Architecture of Convergence of Computing and Networking towards 6G Requirement显示文摘In 6G era,service forms in which computing power acts as the core will be ubiquitous in the network.At the same time,the collaboration among edge computing,cloud computing and network is needed to support edge computing service with strong demand for computing power,so as to realize the optimization of resource utilization.Based on this,the article discusses the research background,key techniques and main application scenarios of computing power network.Through the demonstration,it can be concluded that the technical solution of computing power network can effectively meet the multi-level deployment and flexible scheduling needs of the future 6G business for computing,storage and network,and adapt to the integration needs of computing power and network in various scenarios,such as user oriented,government enterprise oriented,computing power open and so on. | Xiongyan Tang Chang Cao Youxiang Wang Shuai Zhang Ying Liu Mingxuan Li Tao He | 2021 | China Communications2021，18，2： | 20 |
| 9 | The proliferation, differentiation, and mineralization effects of puerarin on osteoblasts in vitro显示文摘AIM: Osteoblasts are key functional cells in the process of bone metabolic balance. Phytoestrogens have an important influence on the proliferation and differentiation of osteoblasts. Puerarin, a plant estrogen, has a wide range concentration in vitro on the function of osteoblasts. The current study investigates the effect of the phytoestrogen puerarin on the proliferation, differentiation, and mineralization of osteoblasts in vitro. METHODS: The calvaria bone of eight-ten Wistar rats which were born within 24 h were obtained in aseptic condition. After enzyme digestion, isolation, purified osteoblasts of rats were cultured for further study. The cells of the first to third generation were divided into a control group and a puerarin-treated group with 10-3–10-10 mol·L-1 puerarin. The cells were exposed to the medium containing a low level of carbohydrates, 10%(V/V) FBS for 24 h. After 1 to 4 days of culture, the OD values on the proliferation of osteoblasts in each group were determined by microplate reader. The cells were cultured in the medium containing 50 μg·mL-1 vitamin C, 10-2 mol·L-1 sodium glycerophosphate, 10% FBS and the medium was changed every 3 to 4 days. After 2 to 8 days of culture, expression of alkaline phosphatase were tested and compared by microplate reader. The mineral nodes of osteoblasts were dyed using alizarin red or improved Von Kossa way after four weeks. RESULTS: Compared with those in the 10-5–10-9 mol·L-1 puerarin, the proliferation of osteoblasts, the expression of alkaline phosphatase, and the number of mineral nodes of osteoblasts were significantly decreased in the control group. The increase was the fastest in the third day, while on the fourth day it was decreased, and arrived at statistical significance compared with the alkaline phosphatase activities and control group. The 10-6 mol·L-1 group was the most distinct, and formed the most mineralized nodule. Compared with the 10-3 mol·L-1 puerarin group, those changes were markedly increased in the control group. CONCLUSIONS: Puerarin has proliferation, differentiation, and mineralization effects on osteoblasts in a dose-dependent manner, and has a double-way effect on the osteoblasts in vitro. A low-dose showed positive effects on the development of osteoblasts, and high-dose puerarin could inhibit the formation of bone. | WANG Chang MENG Mei-Xia TANG Xu-Lei CHEN Ke-Ming ZHANG Li LIU Wei-Ning ZHAO Ying-Yong | 2014 | Chinese Journal of Natural Medicines2014，12，6： | 18 |
| 10 | Advanced Space-based Solar Observatory(ASO-S):an overview显示文摘The Advanced Space-based Solar Observatory(ASO-S)is a mission proposed for the 25 th solar maximum by the Chinese solar community.The scientific objectives are to study the relationships between the solar magnetic field,solar flares and coronal mass ejections(CMEs).Three payloads are deployed:the Full-disk vector Magneto Graph(FMG),the Lyman-αSolar Telescope(LST)and the Hard X-ray Imager(HXI).ASO-S will perform the first simultaneous observations of the photospheric vector magnetic field,non-thermal imaging of solar flares,and the initiation and early propagation of CMEs on a single platform.ASO-S is scheduled to be launched into a 720 km Sun-synchronous orbit in 2022.This paper presents an overview of the mission till the end of Phase-B and the beginning of Phase-C. | Wei-Qun Gan Cheng Zhu Yuan-Yong Deng Hui Li Yang Su Hai-Ying Zhang Bo Chen Zhe Zhang Jian Wu Lei Deng Yu Huang Jian-Feng Yang Ji-Jun Cui Jin Chang Chi Wang Ji Wu Zeng-Shan Yin Wen Chen Cheng Fang Yi-Hua Yan Jun Lin Wei-Ming Xiong Bin Chen Hai-Chao Bao Cai-Xia Cao Yan-Ping Bai Tao Wang Bing-Long Chen Xin-Yu Li Ye Zhang Li Feng Jiang-Tao Su Ying Li Wei Chen You-Ping Li Ying-Na Su Hai-Yan Wu Mei Gu Lei Huang Xue-Jun Tang | 2019 | Research in Astronomy and Astrophysics2019，19，11： | 17 |
| 11 | Reduced Macular Vascular Density in Myopic Eyes显示文摘 | Hua Fan Hao-Yu Chen Hong-Jie Ma Zheng Chang Hai-Quan Yin Danny Siu-Chun Ng Carol Y Cheung Shan Hu Xiang Xiang Shi-Bo Tang Shuang-Nong Li | 2017 | Chinese Medical Journal2017，，4： | 17 |
| 12 | Distribution of C_4 plants along the Northeast China transect and its correlation to the environmental factors显示文摘The distribution of C4 plants and the C4/C3 ratio along Northeast China transect (NECT) have been studied. A model to quantify their relation with environmental factors has been set up. The ratio of the number of C4 plants to that of C3 plants along NECT shows two low and two high trends from east to west, and their distribution is mainly decided by annual average temperature and precipitation. | TANG HaipingOpen Research Laboratory of Environmental Changes and Natural Disaster of the Ministry of National Education, Beijing Normal University , Beijing 100875, Laboratory of Quantitative Vegetation Ecology , Institute of Botany , Chinese Academy of Sciences , Beijing 100093, China | 1999 | Chinese Science Bulletin1999，44，14： | 16 |
| 13 | Discovery of biclonal origin and a novel oncogene SLC12A5 in colon cancer by single-cell sequencing显示文摘单个房间的定序是为描出的一个强大的工具为个性化的癌症管理的同种细胞的关系和识别关键司机基因。这里,我们执行了结肠癌的一个盒子的单个房间的定序的分析。人口遗传分析在肿瘤房间人口识别了二独立克隆。主要肿瘤克隆作为早 oncogenic 事件怀有 APC 和 TP53 变化,而次要的克隆包含了优势的 CDC27 和 PABPC1 变化。在这二克隆的次要的克隆支持的 APC 和 TP53 变化的缺席从二细胞的起源被导出。体的变化等位基因频率系列的检查另外 21 个整个织物的定序 exome 的盒子在结肠癌揭示了同种细胞的起源的异质。下次,我们识别了在单个房间的水平显示出变化的高频率,但是在人口水平展出了低流行的变异的基因 SLC12A5。变异的 SLC12A5 的功能的描述在结肠癌揭示了它的潜在的 oncogenic 效果。我们的学习在单个房间的水平支持提供第一条 exome 宽的证据那结肠癌能具有 biclonal 起源,并且建议在一个队的低流行的变化可以也在单个水平起重要 protumorigenic 作用。 | Chang Yu Jun Yu Xlaotlan Yao Wllham KK Wu Youyong Lu Senwel Tang Xiangchun Li Li Bao Xiaoxing Li Yong Hou Renhua Wu Min Jian Ruoyan Chen Fan Zhang Lixia Xu Fan Fan Jun He Qiaoyi Liang Hongyi Wang Xueda Hu Minghui He Xiang Zhang Hancheng Zheng Qibin Li Hanjie Wu Yan Chen Xu Yang Shida Zhu Xun Xu Huanming Yang Jian Wang Xiuqing Zhang Joseph JY Sung Yingrui Li Jun Wang | 2014 | Cell Research2014，，6： | 16 |
| 14 | Debris Flow Susceptibility Assessment Using a Probabilistic Approach:A Case Study in the Longchi Area, Sichuan Province, China显示文摘The Longchi area with the city of Dujiangyan, in the Sichuan province of China, is composed of Permian stone and diorites and Triassic sandstones and mudstones intercalated with slates. An abundance of loose co-seismic materials were present on the slopes after the May 12, 2008 Wenchuan earthquake, which in later years served as source material for rainfall-induced debris flows or shallow landslides. A total of 48 debris flows, all triggered by heavy rainfall on 13 th August 2010, are described in this paper. Field investigation, supported by remote sensing image interpretation, was conducted to interpret the co-seismic landslides in the debris flow gullies. Specific characteristics of the study area such as slope, aspect, elevation, channel gradient, lithology, and gully density were selected for the evaluation of debris flow susceptibility. A score was given to all the debris flow gullies based on the probability of debris flow occurrence for the selected factors. In order to get the contribution of the different factors, principal component analyses were applied. A comprehensive score was obtained for the 48 debris flow gullies which enabled us to make a susceptibility map for debris flows with three classes. Twenty-two gullies have a high susceptibility, twenty gullies show a moderate susceptibility and six gullies have a low susceptibility for debris flows. | CHANG Ming TANG Chuan ZHANG Dan-dan MA Guo-chao | 2014 | Journal of Mountain Science2014，11，4： | 16 |
| 15 | Antioxidation Activities of Low-Molecular-Weight Gelatin Hydrolysate Isolated from the Sea Cucumber Stichopus japonicus显示文摘Gelatin extracted from the body wall of the sea cucumber (Stichopus japonicus) was hydrolyzed with flavourzyme.Low-molecular-weight gelatin hydrolysate (LMW-GH) of 700-1700 Da was produced using an ultrafiltration membrane bioreactor system.Chemiluminescence analysis revealed that LMW-GH scavenges high free radicals in a concentration-dependent manner;IC50 value for superoxide and hydroxyl radicals was 442 and 285 μg mL-1,respectively.LMW-GH exhibited excellent inhibitory characteristics against melanin synthesis and tyrosinase activity in B16 cells.Furthermore,LMW-GH notably increased intracellular glutathione (GSH),which in turn suppressed melanogenesis.LMW-GH performs antioxidation activity,holding the potential of being used as a valuable ingredient in function foods,cosmetics and pharmaceuticals or nutriceuticals. | WANG Jingfeng WANG Yuming TANG Qingjuan WANG Yi CHANG Yaoguang ZHAO Qin XUE Changhu | 2010 | Journal of Ocean University of China2010，9，1： | 13 |
| 16 | Molecular Typing of Brucella Suis Collected from 1960s to 2010s in China by MLVA and PFGE显示文摘Brucellosis is a bacterial anthropozoonosis usually caused by Brucella abortus, Brucella melitensis, Brucella suis and Brucella canis. Brucella suis, the causative agent of swine brucellosis, is classified into five biovars and preferentially infects different animal hosts [1] . | LI Zhen Jun CUI Bu Yun CHEN Hai CHEN Jing Diao ZHAO Hong Yan PIAO Dong Ri JIANG Hai ZHANG Li TANG Xu KE Chang Wen YAO zhen TIAN Guo Zhong | 2013 | Biomedical and Environmental Sciences2013，26，6： | 13 |
| 17 | Effect of human epididymis protein 4 gene silencing on the malignant phenotype in ovarian cancer显示文摘背景人的 epididymis 能分泌的蛋白质(HE4 ) 4 被证明了是为上皮的卵巢的癌的察觉的有希望的新奇 biomarker。与 CA125 相比, HE4 试金表明了一个改进能力与恶意、良性的疾病在骨盆的质量之间区别。尽管然而, HE4 是在卵巢的癌症的 overexpressed,是众所周知的,在卵巢的癌症的 carcinogenesis 和前进的 HE4 的角色在这研究仍然是 unkown.Methods,我们在卵巢的癌症的 carcinogenesis 和前进探索了 HE4 的角色。我们为 HE4 表示,和使用的 RNA 干扰(RNAi ) 屏蔽了九根卵巢的癌症房间线,我们在 CaoV3 和 SKOV3.ip1 的 silenced HE4 基因表示卵巢的癌症房间线。我们由在 vitro.Results HE4 基因 silencing 检验房间周期, apoptosis,增长和 transwell 迁居 / 侵略在转变显型上估计了 HE4 基因 silencing 的效果导致 G0/G1 拘捕并且在 CaoV3 和 SKOV3.ip1 房间阻止 G1 接近前进到 S 阶段。HE4 在在 vitro.Conclusion HE4 的 SKOV3.ip1 房间的击倒的也禁止的房间增长,移植和侵略可以涉及房间周期的规定并且支持卵巢的癌症移植和侵略。 | ZOU Shu-li CHANG Xiao-hong YE Xue CHENG Hong-yan CHENG Ye-xia TANG Zhi-jian ZHANG Zu-juan GAO Li CHEN Xin-hua CUI Heng | 2011 | Chinese Medical Journal2011，，19： | 12 |
| 18 | The OsAMT1.1 gene functions in ammonium uptake and ammoniumepotassium homeostasis over low and high ammonium concentration ranges显示文摘Rice(Oryza sativa) grown in paddy fields is an ammonium(NH^+_4)-preferring crop;however,its AMT-type NH^+_4transporters that mediate root N acquisition have not been well characterized yet.In this study,we analyzed the expression pattern and physiological function of the OsAMT1.1 gene of the AMT1 subfamily in rice.Os AMT1.1 is located in the plasma membrane and is mainly expressed in the root epidermis,stele and mesophyll cells.Disruption of the Os AMT1.1 gene decreased the uptake of NH^+_4,and the growth of roots and shoots under both low NH^+_4and high NH^+_4conditions.Os AMT1.1 contributed to the short-term(5 min)^(15)NH^+_4influx rate by approximately one-quarter,irrespective of the NH^+_4concentration.Knockout of Os AMT1.1 significantly decreased the total N transport from roots to shoots under low NH^+_4conditions.Moreover,compared with the wild type,the osamt1.1 mutant showed an increase in the potassium(K)absorption rate under high NH^+_4conditions and a decrease under low NH^+_4conditions.The mutants contained a significantly high concentration of K in both the roots and shoots at a limited K(0.1 mmol/L)supply when NH^+_4was replete.Taken together,the results indicated that OsAMT1.1 significantly contributes to the NH^+_4uptake under both low and high NH^+_4conditions and plays an important role in Ne K homeostasis in rice. | Chang Li Zhong Tang Jia Wei Hongye Qu Yanjie Xie Guohua Xu | 2016 | Journal of Genetics and Genomics2016，43，11： | 12 |
| 19 | Detection for disease tipping points by landscape dynamic network biomarkers显示文摘A new model-free method has been developed and termed the landscape dynamic network biomarker(l-DNB) methodology. The method is based on bifurcation theory, which can identify tipping points prior to serious disease deterioration using only single-sample omics data. Here, we show that l-DNB provides early-warning signals of disease deterioration on a single-sample basis and also detects critical genes or network biomarkers(i.e. DNB members) that promote the transition from normal to disease states. As a case study, l-DNB was used to predict severe influenza symptoms prior to the actual symptomatic appearance in influenza virus infections. The l-DNB approach was then also applied to three tumor disease datasets from the TCGA and was used to detect critical stages prior to tumor deterioration using an individual DNB for each patient. The individual DNBs were further used as individual biomarkers in the analysis of physiological data, which led to the identification of two biomarker types that were surprisingly effective in predicting the prognosis of tumors. The biomarkers can be considered as common biomarkers for cancer, wherein one indicates a poor prognosis and the other indicates a good prognosis. | Xiaoping Liu Xiao Chang Siyang Leng Hui Tang Kazuyuki Aihara Luonan Chen | 2019 | National Science Review2019，6，4： | 12 |
| 20 | Covalently closed-circular hepatitis B virus DNA reduction with entecavir or lamivudine显示文摘AIM: To investigate the reduction in hepatitis B virus(HBV) covalently closed-circular DNA(ccc DNA) with entecavir(ETV) or lamivudine(LAM). METHODS: This analysis included patients who had participated in the randomized Phase Ⅲ study ETV-022 comparing ETV vs LAM in nucleos(t)ide-naive, HBe Agpositive patients. Patients received ETV(0.5 mg daily) or LAM(100 mg daily) for a minimum of 52 wk. Patients were eligible to participate in this sub-study if they had paired biopsies at baseline and week 48 with evaluable measurements for hepatic HBV ccc DNA and total hepatic HBV DNA. The main objective was to compare changes in hepatic HBV ccc DNA and total hepatic HBV DNA at week 48 of ETV or LAM treatment, which was a secondary endpoint of study ETV-022. Additional post hoc analyses included linear regression analyses to assess associations of baseline levels and on-treatment changes of ccc DNA with other baseline factors [sex,age, serum HBV DNA, alanine aminotransferase(ALT), Knodell necroinflammatory score, Ishak fibrosis score, total hepatic HBV DNA, and HBV genotype], or ontreatment factors(changes from baseline at week 48 in serum HBV DNA, ALT, Knodell necroinflammatory score, Ishak fibrosis score, total hepatic HBV DNA, and HBe Ag loss at week 48).RESULTS: Overall, 305 patients(ETV = 159; LAM = 146) of ETV-022 had paired baseline and week 48 liver biopsies with evaluable measurements for hepatic HBV ccc DNA and total hepatic HBV DNA, and were included in this analysis. Baseline demographics and disease characteristics were comparable between the two arms. After 48 wk, ETV resulted in significantly greater reductions in hepatic HBV ccc DNA [-0.9 log10 copies/human genome equivalent(HGEq) vs-0.7 log10 copies/HGEq; P = 0.0033] and total hepatic DNA levels(-2.1 log10 copies/HGEq vs-1.6 log10 copies/HGEq; P < 0.0001) than LAM. Virologic, biochemical, and histologic response rates at week 48 were also greater with ETV than with LAM. Baseline HBV ccc DNA levels were positively associated with baseline levels of serum HBV DNA and total hepatic HBV DNA, and negatively associated with HBV genotype F. On-treatment changes in HBV ccc DNA levels were negatively associated with baseline levels of serum HBV DNA and baseline ALT, and were positively associated with on-treatment changes in the levels of serum HBV DNA, total hepatic HBV DNA levels, and ALT, change in Knodell necroinflammatory score, and HBe Ag loss.CONCLUSION: Forty-eight weeks of ETV resulted in greater reductions in ccc DNA and total hepatic HBV DNA than LAM, but long-term therapy may be needed for ccc DNA elimination. | Scott Bowden Stephen Locarnini Ting-Tsung Chang You-Chen Chao Kwang-Hyub Han Robert G Gish Robert A de Man Miao Yu Cyril Llamoso Hong Tang | 2015 | World Journal of Gastroenterology2015，21，15： | 11 |
您所在的机构暂未开通此部分的功能，请联系管理员