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| 1 | Generation of haploid embryonic stem cells from Macaca fascicularis monkey parthenotes显示文摘在从经由单性生殖和男性单性生殖的老鼠的胚胎的干细胞(haESCs ) 启用了在修改基因的动物的哺乳动物的房间和代的基因屏蔽的 haploid 的推导的最近的成功。然而, haESCs 是否能从首领被导出,仍然保持未知。这里,我们从 Macaca fascicularis 猴子的单性生殖的胚囊报导 haESCs 的推导。这些房间,作为 PG-haESCs 称为,是 pluripotent 并且能在 vitro 或在 vivo 区分到三胚胎的细菌层的房间。有趣地,一根猴子 PG-haESC 线(MPH1 ) 的 haploidy 与另一个(MPH2 ) 的相比是更稳定的,作为没有排序的激活荧光的房间(FACS ) ,由 haploid 房间的存在出现超过 140 天 haploid 房间的丰富。重要地,转基因的猴子 PG-haESC 线能被 lentivirus- 和 piggyBac 产生调停 transposon 的基因转移。而且,基因屏蔽在猴子 PG-haESCs 是可行的。我们的结果证明 PG-haESCs 能从猴子被产生,提供为在首领的基因分析的一个理想的工具。 | Hui Yang Zhen Liu Yu Ma Cuiqing Zhong Qi Yin Chikai Zhou Linyu Shi Yijun Cai Hanzhi Zhao Hui Wang Fan Tang Yan Wang Chenchen Zhang Xin-yuan Liu Dongmei Lai Ying Jin Qiang Sun Jinsong Li | 2013 | Cell Research2013，23，10： | 22 |
| 2 | Quantitative analysis of Pb adsorption on sulfhydryl-modified biochar显示文摘Biochar is an effective absorbent for remediating heavy metal contaminated soil,but functional optimization is still needed to improve its performance in field application.Here,we characterized the physical structures and surface chemical properties of raw wood biochar and palm biochar(WB and PB)and the corresponding sulfhydryl-modified biochar(SWB and SPB).Their adsorption capacity for Pb was evaluated by combining thermodynamic and kinetic adsorption at 0.01 mol/L KCl and corresponding model simulation.The results demonstrated successful grafting of sulfhydryl groups onto the biochar,which dramatically reduced the specific surface area(SSA)and pore volume of biochar.The pKa in the surface complexation model(SCM)indicated similar proton affinity between sulfhydryl groups and original functional groups on the biochar.SCM could satisfactorily fit the Pb adsorption behaviors,and model analysis revealed that Pb tended to be adsorbed on low-proton affin-ity sites at low pH,but high-proton affinity sites became dominant in Pb adsorption with increasing pH and adsorbed almost all Pb ions at pH>7.0.Besides,the Pb adsorption density of SWB and SPB was improved by 8.86 and 3.64 folds relative to that of WB and PB,respectively.Over 90% of initially added Pb ions were removed in 1440 and 720 min by raw and sulfhydryl-modified biochar,respectively,indicating that sulfhydryl modification accelerated the Pb adsorption of biochar.These results suggest that site density,SSA and pore structure of biochar play crucial roles in heavy metal adsorption,and sulfhydryl modification may improve the performance of biochar in remediating heavy metal contaminated soil. | Juan Xiong Mengge Zhou Chenchen Qu Daohai Yu Chang Chen Mingxia Wang Wenfeng Tan | 2021 | Biochar2021，3，1： | 5 |
| 3 | Cu3(PO4)2: Novel Anion Convertor for Aqueous Dual‑Ion Battery显示文摘Electrode materials which can reversibly react with anions are of interest for aqueous dual-ion batteries.Herein,we propose a novel anion electrode,Cu3(PO4)2,for constructing an aqueous dual-ion cell.The Cu3(PO4)2 electrode can operate in a quasi-neutral condition and deliver a reversible capacity of 115.6 mAh g^−1 with a well-defined plateau at−0.17 V versus Ag/AgCl.Its reaction mechanism shows that Cu3(PO4)2 decomposes into Cu2O and subsequently is converted into Cu during the initial discharge process.In the following charge process,Cu is oxidized into Cu2O.It suggests Cu3(PO4)2 reacts with OH−ions instead of PO43−ions after the initial discharge process and its potential thereby depends upon the OH−ions concentration in electrolyte.Additionally,an aqueous dual-ion cell is built by using pretreated Cu3(PO4)2 and Na0.44MnO2 as anode and cathode,respectively.During cycling,OH−ions and Na^+ions in electrolyte can be stored and released.Such a cell can provide a discharge capacity of 52.6 mAh g^−1 with plateaus at 0.70 and 0.45 V,exhibiting the potential of application.This work presents an available aqueous dual-ion cell and provides new insights into renewable energy storage and adjustment of the OH−ions concentration in aqueous buffer solution. | Haoxiang Yu Chenchen Deng Huihui Yan Maoting Xia Xikun Zhang Zhen‑Bo Wang Jie Shu | 2021 | Nano-Micro Letters2021，13，3： | 3 |
| 4 | Dahuang Fuzi decoction reduces inflammation levels and alleviates intestinal mucosal barrier damage in septic rats显示文摘Objective:To investigate the protective effect of Dahuang Fuzi Decoction(DHFZD),a traditional Chinese prescription,at alleviating sepsis-induced inflammation and gut barrier damage in rats.Methods:Forty clean-grade male Sprague-Dawley rats were divided randomly into three groups:normal control group(NCG,n?10),model control group(MCG,n?15)and DHFZD-treated group(DHFZDG,n?15).NCG rats were sham operated on and used as the controls,whereas MCG and DHFZDG rats were used to replicate the rat sepsis model using cecal ligation and puncture(CLP).The DHFZDG rats received DHFZD by gavage(4.5 mg/g of body weight)2 h prior to CLP and after its successful induction,while the NCG and MCG rats received equivalent amounts of sterilized water by gavage.All rat groups were starved and had free access to water.At 24 h post-experimental set up,the mortality of rats in each group was recorded,and peritoneal inflammation assessment and pathological changes related to the intestinal mucosal injury index(IMII)in the surviving rats were evaluated.D-lactic acid,tumor necrosis factor(TNF)-a,interleukin(IL)-6 and IL-10 peripheral blood concentrations,along with secretory immunoglobulin A(sIgA)in the intestinal mucosa were evaluated by enzyme-linked immunosorbent assays.Gut microbes were detected using 16S rRNA gene sequencing.Results:DHFZD reduced sepsis-related mortality in the rats.Moreover,it alleviated peritoneal inflammation and pathological changes according to the IMII.DHFZD reduced serum procalcitonin,TNF-a and IL-6 concentrations,but not the IL-10 concentration.It also reduced serum D-lactic acid and increased sIgA concentrations in intestinal mucosa.Notably,DHFZDG restored gut microbiota diversity and regulated the decrease in Bacteroidetes induced by sepsis,compared with the MCG rats.Conclusion:DHFZDG may play a protective role in sepsis by alleviating sepsis-induced inflammation and gut barrier damage in rats. | Fusheng Liu Chunyue Yu Jin Liu Nadia Johnson Yin Zhang Zeqi Su Chenchen Sun Xia Ding | 2020 | Journal of Traditional Chinese Medical Sciences2020，7，1： | 3 |
| 5 | Congestive heart failure in COX2 deficient rats显示文摘Non-steroidal anti-inflammatory drugs(NSAIDs)inhibit prostaglandin(PG)formation by targeting cyclooxygenase(COX)1 and 2.Long-term use of NSAIDs that selectively inhibit COX2 increases the risk for thrombotic events,cardiac failure,and hypertension.However,the underlying mechanisms remain unclear.In this study,COX1-and COX2-deficient rats were created via Cas9/RNA-mediated gene targeting.DNA genotyping and Western blot analysis confirmed successful generation of COX1^(-/-) and COX2^(-/-) rats.Adult COX1^(-/-) rats grew normally,while more than 70%of COX2^(-/-) rats after wean died within 2 months.Echocardiography showed markedly reduced left ventricular ejection fraction and fractional shortening in adult COX2^(-/-) rats compared to those in wildtype(WT)controls.Histological analysis revealed accumulation of inflammatory cells and severe interstitial and perivascular fibrosis in COX2^(-/-)cardiac tissues.Moreover,cardiac ATP and acetyl-Co A production was dramatically decreased in COX2^(-/-) rats.Consistently,the expression of genes related to mitochondrial oxidation,such as those that encode for subunits of pyruvate dehydrogenase complex and acyl Co A dehydrogenases,were downregulated,while glycolytic hexokinase 1(HK1)was upregulated in COX2^(-/-) heart tissues.These observations indicate that COX2-deficient rats developed spontaneously heart failure,likely as a result of dysregulated cardiac energy metabolism. | Qiangyou Wan Deping Kong Qian Liu Shumin Guo Chenchen Wang Yan Zhao Zun-Ji Ke Ying Yu | 2021 | Science China(Life Sciences)2021，64，7： | 3 |
| 6 | Overexpression of programmed cell death-1 (PD-1) affects circulatory Th1 and Th2 cells in patients with cardiac arrest in the early period after the return of spontaneous circulation显示文摘To the Editor:Cardiac arrest(CA)is the leading cause of morbidity and mortality among hospitalized patients globally and has low rates of overall survival.After the return of spontaneous circulation(ROSC),patients with CA and systemic ischemia/reperfusion injury can manifest as sepsis-like syndromes and immune disorders that increase the risk of infections.CD4^(+)T lymphocytes play an important role in organ ischemia-reperfusion injury.T-helper(Th)types 1 and 2 cells are classical subsets of CD4^(+)T cells.Extensive research on their functions in infectious and immune-related inflammatory diseases indicates that both subsets participate in host defense and direct immune responses.Data from CA/cardiopulmonary resuscitation porcine models and patients have revealed imbalances in Th1/Th2 cell ratio.[1]Therefore,Th1 and Th2 cells are important for understanding post-ROSC immune dysfunction in patients with CA. | Yanan Yu Miaorong Xie Jiabao Li Chenchen Hang Fei Shao Chunsheng Li | 2022 | Chinese Medical Journal2022，，1： | 2 |
| 7 | Extreme Temperature Change of the Last 110 Years in Changchun,Northeast China显示文摘In China and East Asia,the long-term continuous observational data at daily resolution are insufficient,and thus there is a lack of good understanding of the extreme climate variation over the last 100 years plus.In this study,the extreme temperature indices defined by ETCCDI(Expert Team on Climate Change Detection and Indices)and local meteorological administrations were analyzed for Changchun City,Northeast China,by using the daily maximum temperature(Tmax)and daily minimum temperature(Tmin)over 1909?2018.The results showed that extreme cold events,such as cold days,cold nights,frost days,icing days,and low temperature days,decreased significantly at rates of?0.41 d(10 yr)^?1,?1.45 d(10 yr)^?1,?2.28 d(10 yr)^?1,?1.16 d(10 yr)?1 and?1.90 d(10 yr)^?1,respectively.Warm nights increased significantly at a rate of 1.71 d(10 yr)^?1,but warm days decreased slightly and the number of high temperature days decreased at a rate of?0.20 d(10 yr)?1.The frequency of cold surge events increased significantly at a rate of 0.25 d(10 yr)^?1,occurring mainly from the mid-1950s to late-1980s.The average Tmax,average Tmin and extreme Tmin increased at rates of 0.09℃(10 yr)^?1,0.36℃(10 yr)^?1 and 0.54℃(10 yr)^?1,respectively;and extreme Tmax decreased significantly at a rate of?0.17℃(10 yr)^?1.In 1909?2018,1951?2018 and 1979?2018,the indices related to cold events decreased,while the trends of the indices related to warm events were different for different periods. | Xiujing YU Guoyu REN Panfeng ZHANG Jingbiao HU Ning LIU Jianping LI Chenchen ZHANG | 2020 | Advances in Atmospheric Sciences2020，37，4： | 2 |
| 8 | Recent progress in small molecule fluorescent probes for nitroreductase显示文摘Nitroreductase(NTR) is a member of flavin-containing enzymes that exists widely in bacteria. Hypoxia,which is a characteristic of locally advanced solid tumors, resulting from an imbalance between oxygen consumption and supply, can result in NTR overexpression. Using either nicotinamide adenine dinucleotide(NADH) or nicotinamide adenine dinucleotide phosphate(NADPH) as a source of reducing equivalents, NTR can catalyze the reduction of nitroaromatic compounds to the corresponding amines.Based on this reduction mechanism, NTR can be applied not only in the bioremediation and degradation of organic nitrogen compounds, but also in the development of NTR-targeted fluorescent probes to detect the hypoxic status of cancer cells. This review aims to provide a summary of the progress in fluorescent probes for NTR in recent years and elucidate the main fluorescent mechanisms that have been applied to design probes. | Wenjing Qin Chenchen Xu Yanfei Zhao Changmin Yu Sheng Shen Lin Li Wei Huang | 2018 | Chinese Chemical Letters2018，29，10： | 2 |
| 9 | Targeted metabolic analysis of nucleotides and identification of biomarkers associated with cancer in cultured cell models显示文摘Cancer,like other diseases accompanied by metabolic changes,shows characteristic DNA/RNA modifications and activities of modifying enzymes,resulting in fluctuations in nucleoside levels.In this study,we undertook targeted metabolomic analyses of nucleotides in different cancer cell culture models using a sensitive and reproducible ion-pair HPLC method.The experimental data were analyzed by principal component analysis(PCA)to identify potential biomarkers in cancer cells,and statistical significance was determined by one-way analysis of variance.As a result,a clear differentiation of normal and tumor cells into two clusters was shown,indicating abnormal metabolism of nucleotides in tumor cells.Six variables(AMP,UDP,CTP levels with a significance of Po0.05;ATP,UTP and GMP levels with a significance of Po0.01)were considered as potential biomarkers;the content of AMP,UTP,GMP and ATP was significantly higher in cancer cells.The receiver operating characteristic(ROC)curve analysis allowed us to discriminate normal cells from tumor cells based on area under the curve(AUC).The sequence of their AUC values were:ATP(0.979)4UTP(0.938)4CTP¼GMP(0.896)4AMP(0.812)4UDP(0.792),so we conclude that ATP and UTP are the best potential biomarkers in tumor cells.This study may provide a valuable tool for studying minute alterations of intracellular nucleotide pools induced by anticancer/antiviral drugs,diseases or environmental factors. | Chenchen Zhang Zheng Liu Xi Liu Lan Wei Yanjie Liu Jing Yu Lixin Sun | 2013 | Acta Pharmaceutica Sinica B2013，3，4： | 2 |
| 10 | A model for gas transport in microfractures of shale and tight gas reservoirs显示文摘 | Keliu Wu Xiangfang Li Chenchen Wang Zhangxin Chen Wei Yu | 2015 | AIChE J2015，，6： | 1 |
| 11 | Abnormal meiosis in fertile and sterile triploid cyprinid fish显示文摘Meiosis is the key process for producing mature gametes. A natural fertile triploid Carassius auratus population(3nDTCC) and an artificially derived sterile triploid crucian carp(3nCC) have been previously observed, providing suitable model organisms for investigating meiosis characteristics in triploid fish. In the present study, the microstructures and ultrastructures of spermatogenesis were studied in these fishes. Td T-mediated d UTP nick end labeling detection was performed to investigate the apoptosis of spermatocytes. Fluorescence in situ hybridization was employed to trace chromatin pairing. In addition, the m RNA expressions of cell cycle-related genes(i.e., cell division control 2 and cell cycle protein B) were determined by quantitative realtime polymerase chain reaction to illustrate the molecular mechanism of abnormal meiosis in the 3nCC. The results showed that the 3nCC undergoes an irregular prophase I, with the chromosomes distributed in a unipolar radial manner and exhibiting partial pairing, hindered metaphase I, and degenerated cells in the subsequent stages. Meanwhile, the 3nDTCC presented a relatively regular meiotic prophase I with complete conjugate chromosome pairs and chromosomes distributed along the karyotheca,which were presented as a ring structure by slicing. Only the spreads with 130–150 irregular chromosomes can be easily detected in the 3nDTCC, suggesting that it may undergo an abnormal metaphase I. This study provides new insights into the meiosis of fertile and sterile triploid cyprinid fish. | Chun Zhang Qi Li La Zhu Wangchao He Conghui Yang Hui Zhang Yu Sun Luojing Zhou Yuandong Sun Shurun Zhu Chang Wu Min Tao Yi Zhou Rurong Zhao Chenchen Tang Shaojun Liu | 2021 | Science China(Life Sciences)2021，64，11： | 1 |
| 12 | Synthesis of a zeolite mem- brane as a protective layer on a metallic Pd composite membrane for hydrogen purification 显示文摘 | Yu Jiafeng Qi Chenchen Zhang Jixian | 2015 | Journal of Materials Chemistry A2015，3，9： | 1 |
| 13 | A genetic algorithm based entity resolution approach with active learning显示文摘 | Chenchen SUN Derong SHEN Yue KOU Tiezheng NIE Ge YU | 2017 | Frontiers of Computer Science2017，11，1： | 1 |
| 14 | Charac- terization of hepatitis E virus infection in tree shrew ( Tupaia be- langeri chinensis) 显示文摘 | YU WENHAI YANG CHENCHEN BI YANHONG | 2016 | BMC Infect Dis2016，16，1： | 1 |
| 15 | Effects of plant diversity on greenhouse gas emissions in microcosms simulating vertical constructed wetlands with high ammonium loading显示文摘Wastewater with relatively high nitrogen concentrations is a major source of nitrous oxide(N_2O) and methane(CH_4) emissions and exerts multiple stresses on the environment.Studies have shown that plant diversity plays an important role in ecosystem functioning.However, the effects of plant species diversity on CH_4 and N_2O emissions under high ammonium(NH_4^+-N) loading rates remain unclear. In this study, a microcosm experiment simulating vertical constructed wetlands supplied with high NH_4^+-N water levels was established. The treatments included four species richness levels(1, 2, 3, 4) and 15 species compositions. There was no significant relationship between species richness and N_2O emissions. However, N_2O emissions were significantly reduced by specific plant species composition. Notably, the communities with the presence of Rumex japonicus L. reduced N_2O emissions by 62% compared to communities without this species. This reduction in N_2O emissions may have been a result of decreased N concentrations and increased plant biomass. CH_4 emissions did not respond to plant species richness or species identity.Overall, plant species identity surpassed species richness in lowering N_2O emissions from constructed wetlands with high NH_4^+-N water. The results also suggest that communities with R. japonicus could achieve higher N removal and lower greenhouse gas emissions than other wetland species. | Wenjuan Han Guiying Luo Bin Luo Chenchen Yu Hai Wang Jie Chang Ying Ge | 2019 | Journal of Environmental Sciences2019，31，3： | 1 |
| 16 | Modulating the C-terminus of DEP1 synergistically enhances grain quality and yield in rice显示文摘Grain shape is a key breeding target that influences both grain yield and appearance quality in rice. Breeding for long-slender grains is an effective strategy to improve grain quality (Jain et al., 2004;Wang et al., 2012, 2015a). In the past two decades, several quantitative trait loci (QTLs) responsible for grain size and shape have been identified, including GS3 (Fan et al., 2006), GS5 (Li et al., 2011). | Haixiang Huang Yafeng Ye Wenzhen Song Qi Li Ruixi Han Chenchen Wu Shuoxun Wang Jianping Yu Xueying Liu Xiangdong Fu Qian Liu Kun Wu | 2022 | Journal of Genetics and Genomics2022，49，5： | 1 |
| 17 | circ_0003204 regulates the osteogenic differentiation of human adipose-derived stem cells via miR-370-3p/HDAC4 axis显示文摘Human adipose-derived stem cells(hASCs)are a promising cell type for bone tissue regeneration.Circular RNAs(circRNAs)have been shown to play a critical role in regulating various cell differentiation and involve in mesenchymal stem cell osteogenesis.However,how circRNAs regulate hASCs in osteogenesis is still unclear.Herein,we found circ_0003204 was significantly downregulated during osteogenic differentiation of hASCs.Knockdown of circ_0003204 by si RNA or overexpression by lentivirus confirmed circ_0003204 could negatively regulate the osteogenic differentiation of hASCs.We performed dual-luciferase reporting assay and rescue experiments to verify circ_0003204 regulated osteogenic differentiation via sponging miR-370-3p.We predicted and confirmed that miR-370-3p had targets in the 3′-UTR of HDAC4 m RNA.The following rescue experiments indicated that circ_0003204 regulated the osteogenic differentiation of hASCs via miR-370-3p/HDAC4 axis.Subsequent in vivo experiments showed the silencing of circ_0003204 increased the bone formation and promoted the expression of osteogenic-related proteins in a mouse bone defect model,while overexpression of circ_0003204 inhibited bone defect repair.Our findings indicated that circ_0003204 might be a promising target to promote the efficacy of hASCs in repairing bone defects. | Liyuan Yu Kai Xia Jing Zhou Zhiai Hu Xing Yin Chenchen Zhou Shujuan Zou Jun Liu | 2022 | International Journal of Oral Science2022，14，3： | 1 |
| 18 | Hypoxia inducible factor‐1α gene polymorphism G1790A and its interaction with tobacco and alcohol consumptions increase susceptibility to hepatocellular carcinoma显示文摘 | Pei‐ChingHsiao Mu‐KuanChen Shih‐ChiSu Kwo‐ChangUeng Yi‐ChenChen Yi‐HsienHsieh Yu‐FanLiu Hsiu‐TingTsai Shun‐FaYang | 2010 | Oncol2010，，2： | 1 |
| 19 | Distributed Secure Storage Scheme Based on Sharding Blockchain显示文摘Distributed storage can store data in multiple devices or servers to improve data security.However,in today’s explosive growth of network data,traditional distributed storage scheme is faced with some severe challenges such as insufficient performance,data tampering,and data lose.A distributed storage scheme based on blockchain has been proposed to improve security and efficiency of traditional distributed storage.Under this scheme,the following improvements have been made in this paper.This paper first analyzes the problems faced by distributed storage.Then proposed to build a new distributed storage blockchain scheme with sharding blockchain.The proposed scheme realizes the partitioning of the network and nodes by means of blockchain sharding technology,which can improve the efficiency of data verification between nodes.In addition,this paper uses polynomial commitment to construct a new verifiable secret share scheme called PolyVSS.This new scheme is one of the foundations for building our improved distributed storage blockchain scheme.Compared with the previous scheme,our new scheme does not require a trusted third party and has some new features such as homomorphic and batch opening.The security of VSS can be further improved.Experimental comparisons show that the proposed scheme significantly reduces storage and communication costs. | Jin Wang Chenchen Han Xiaofeng Yu Yongjun Ren R.Simon Sherratt | 2022 | Computers, Materials & Continua2022，，3： | 1 |
| 20 | Dynamic evaluation of land productivity in China显示文摘For making rational and scientific land-use planning,an inventory indicator of land productivity subject to biophysical limitations and human disturbance is required.This study introduces agricultural land productivity(ALP)as the dynamic indicator to measure land productivity,involving factors that affect agricultural production,such as characteristics of climate,soil,and terrain.As a case study of this dynamic indicator,ALP was estimated at county level and aggregated into provincial level in the whole of China.The result shows that ALP increased during 2000-2010 in China,and the ratios of ALP to the actual grain yield were 65.40%and 66.38%in the years 2000 and 2005,respectively.The ALP is an effective dynamic indicator to estimate the land productivity and to represent the basic conditions about the supply of water,energy,nutrients,and the physical basis for plant growth,as well as the local input.ALP estimation could provide supporting information for policy-making about land production planning and land-use management. | Rui Yu Xiangzheng Deng Zhe Yan Chenchen Shi | 2013 | Chinese Journal of Population,Resources and Environment2013，11，3： | 0 |
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