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| 1 | Effect of arsenic trioxide on rat hepatocarcinoma and its renal cytotoxicity显示文摘AIM: To study the effect of arsenic trioxide (As2O3) on rat experimental hepatocarcinoma and its renal cytotoxicity.METHODS: The hepatocarcinoma model was established by diethaylnitrosamine perfusion in stomach of 120 Wistar rats, and the treatment began at the end of 20 weeks.Before the treatment, the rat models were randomly divided into 5 groups. In the treatment groups, three doses of As2O3 were injected into rat abdominal cavity, the total time of drug administration was 4 weeks. Cisplatin control or the blank group was injected into abdominal cavity with equal amount of cisplatin or saline at the same time,respectively. On the 7th, 14th and 28th day after the treatment, the hepatocarcinoma nodules were obtained and the morphologic changes of hepatocarcinoma cells were observed under light and electron microscopes;Immunohistochemistry (S-P methods) was employed to detect the expression of bcl-2, bax and PCNA in hepatocarcinoma tissues; flow cytometry (TUNEL assay)was used to detect the apoptosis of liver cancer cells and the change of cytokinetics. On the 28th day, the kidneys were obtained and their histologic changes were observed under light microscope, and immunohistochemistry (SP stain) was also employed to detect the expression of bcl-2and PCNA. Cisplatin and saline solution were used as the control.RESULTS: As2O3 could induce the apoptosis of rat liver cancer cells and exhibited typical morphologic changes.The incidence of apoptosis of hapatocarcinoma cells was elevated (P=0.001). The elevation was the most higher in the group of middle-dose of As2O3 (1 mg.kg-1), significantly higher than that of the other arsenic groups and the controls (P=0.001). Large dose of As2O3 (5 mg.kg-1) was able to arise the incidence of apoptosis, but also produced a large amount of necrosis and inflammatory reaction. Middle dose of As2O3 dramatically increased the cell number in G2/M phase (P=0.0001), and apoptosis happened apparently.The expression of bcl-2 and bax was related to the dose of As2O3. With the up-regulation of apoptotic incidence, the ratio of bcl-2/bak decreased. But the incidence of apoptosis was not the highest status and the ratio of bcl-2/bax was at the lowest when the highest-dose of As2O3 was used.There was significant difference among the PCNA indexes (PCNA L1) of the five groups. Of them, three arsenic groups all showed decrease of different degrees, and this downregulation was most obvious in group A. There was significant difference among the three groups (P=0.016).Under the light microscope, the rat kidney in the cisplatin group exhibited tubular epithelium swelling and degeneration, protein casts in collecting tubules; While all arsenic groups didn't show the significant changes (P=0.013).In the arsenic groups, the expression of bcl-2 in the renal tubular epithelium was increased (P=0.005), no obvious changes happened to PCNA L1. But in the group of cisplatin,the PCNA L1 increased significantly (P=0.001).CONCLUSION: AS2O3 can induce apoptosis of rat hepatocellular carcinoma cells. And there is optimum dose;too high dose will induce the cytotoxic effect, while certain dose of As2O3 is able to block the cell cycle at G2/M phase.As2O3 had the most remarkable influence on G2/M cells,and it can also induce apoptosis to cells at other phases.As2O3 can restrain the proliferation of rat hepatocellular carcinoma cells, in a dose-time dependent manner.Compared with cisplatin, As2O3 didn't show obvious renal toxicity, which was related to the increasing expression of bcl-2 in renal tubular epithelium, the inhibition of apoptosis and the anti-oxidation effects. | Shao-Shan Wang Ti Zhang Xi-Lu Wang Li Hong Department of Surgery of Dagang Hospital 300270,Tianjin,China Qing-Hui Qi Department of Chinese and Western Integral Surgery of Master Hospital of Tianjin Medical University 300052,Tianjin,China | 2003 | World Journal of Gastroenterology2003，9，5： | 17 |
| 2 | The temporal and spatial distribution of ancient rice in China and its implications显示文摘The relics of ancient rice have been regarded as the most important objective evidence of the origina- tion and spread of rice cultivation.Based on the records of 280 rice relics sites and the rice cropping regionalization as well as the distribution map of paddy soils,the current study compiled the temporal and spatial distribution map of ancient rice distribution in China.The map shows that the distribution of ancient rice is spatially extensive and meantime comparatively concentrated,temporarily covering a long and relatively continuous time-span.The rice relics in the Central China double and single rice cropping regions are among the earliest and the most abundant ones,possessing continuity in time sequence.Combined with the discovery of ancient rice and paddy filed relics,soil micromorphology, pollen combination and element geochemistry,it is suggested that Central China was the origin center of rice cultivation in China.Rice had been spread to the rest part of China in three major waves,also to the East Asian part like Korea and Japan.The temporal and spatial distribution of ancient rice reflects the past environmental change,which is also meaningful to the current rice regionalization and plan- ning as well as food security in China. | GONG ZiTong CHEN HongZhao YUAN DaGang ZHAO YuGuo WU YunJin ZHANG GanLin | 2007 | Chinese Science Bulletin2007，52，8： | 11 |
| 3 | Effect of Preparation Methods on Mechanical Properties of PVA/HA Composite Hydrogel显示文摘Poly(vinyl alcohol) (PVA)/hydroxyapatite (HA) composite hydrogel specimens were prepared with 15% PVA and 1%,2%, 3%, 4% and 5% HA by repeated freezing-thawing. The tests of static and dynamic mechanical properties were carried out todiscuss the influence of different contents of HA and freezing-thawing cycles on the mechanical properties of PVA/HA compositehydrogel. The results of static mechanical tests showed that the PVA/HA composite hydrogel with 3% HA and ninefreezing-thawing cycles had excellent stress relaxation properties, higher relaxation ratio, lower stress equilibrium value andpresented better properties of creep and recovery. The results of dynamic mechanical test showed that the PVA/HA compositehydrogel with nine freezing-thawing cycles had higher storage modulus and loss modulus, so was the PVA/HA compositehydrogel with 3% HA. | Dekun Zhang~1, Junjie Duan~1, Dagang Wang~2, Shirong Ge~2 1. School of Materials Science and Engineering, China University of Mining and Technology Xuzhou 221116, P. R. China 2. School of Mechanical and Electrical Engineering, China University of Mining and Technology, Xuzhou 221116, P. R. China | 2010 | Journal of Bionic Engineering2010，7，3： | 8 |
| 4 | Synthesis and Characterization of PVA-HA-Silk Composite Hydrogel by Orthogonal Experiment显示文摘 | Dekun Zhang Kai Chen Lin Wu Dagang Wang Shirong Ge | 2012 | Journal of Bionic Engineering2012，9，2： | 6 |
| 5 | Tension leg platform project execution显示文摘The first floating platform concept design work for South China Sea is undergoing in DMAR's office now. This tension leg platform has potential to become the first advanced floating production platform project. Project execution is always a challenge for floating system. This paper focuses on the critical elements of project execution for tension leg platform,and studies potential implications to future oil and gas exploration in South China Sea. There are many factors affecting successful execution of floating system project,including technical issues, engineering management,interface management,etc. There are also failure examples of project execution in the industry. The author has participated 28 large detailed projects and has gained extensive experience on floating projects,with ample hands-on project experiences. A detailed tension leg platform project study example and discussions in depth are presented for future project execution in China deepwater development. | Zhang Dagang Wang Chunsheng Zhang Tianyu | 2013 | Engineering Sciences2013，11，4： | 5 |
| 6 | Suppression of Vortex-Induced Vibration by Fairings on Marine Risers显示文摘Vortex-induced vibration is quite common during the operation of offshore risers or umbilical cables,commonly leading to serious damage to risers and reduced service life.Vortex-induced vibration of the offshore risers could be effectively suppressed by fairing devices.In this paper,a newly developed vortex-induced vibration fairing and large eddy simulation model of the FLUENT software were used for numerical analysis,experimental research and stimulating vortex-induced vibration at 0.1–2 ms^-1.The data of the numerical model with fairing was compared and analyzed to study the vortex shedding frequency at different Reynolds numbers and changes in drag and lift coefficients.The displacement state of 12 in risers with and without fairing was experimentally tested using a five degree-of-freedom balance.The vortex-induced vibration effect of the fairing was tested at different velocities.The result shows the drag reduction effect of the fairing is more obvious when the flow velocity is 0.4–1.2 ms^-1 and the maximum drag reduction reaches 55.6%when the flow velocity is 0.6 ms^-1.Additionally,the drag reduction effect was obvious when the flow velocity was greater than 1.3 ms^-1 and less than 0.3.The result indicates that the developed 12 in fairing,with good potential in engineering applications,has good vortex-induced vibration-suppression effects. | KANG Yongtian XIAO Wensheng WANG Quanbin ZHANG Dagang ZHAO Jun | 2020 | Journal of Ocean University of China2020，19，2： | 4 |
| 7 | Deepwater oil and gas field development in South China Sea显示文摘This paper focuses on potential development models of future oil and gas exploration in South China Sea. A detailed study of current development models worldwide is performed through some examples of industry installed/ongoing projects and major technical issues encountered during these practice. Key technologies are discussed for the success of field development. Some of the technologies and field development experience can be used for South China Sea project. Several models are studied in field development for different scenarios,including marginal field,large oil field and gas field. With the massive investment activities,continued improved technologies,and rapidly growing pool of professionals,the offshore industry in China will soon encounter a golden period. | Zhang Dagang Wybro Pieter Kang Yongtian | 2013 | Engineering Sciences2013，11，4： | 4 |
| 8 | Preparation,Characterization and Hot Storage Stability of Asphalt Modified by Waste Polyethylene Packaging显示文摘Waste polyethylene packaging(WPE) was used to modify asphalt,and hot storage stability of the modified asphalt was studied in this paper.The morphological change and component loss of WPE modified asphalt were characterized by fluorescence microscopy,Fourier transform infrared spectroscopy(FT-IR),differential scanning calorimetry(DSC),thermogravimetry(TG) and isolation testing.In addition,the mechanism of the hot storage stability of WPE modified asphalt was discussed.The results showed that the modification of asphalt with WPE was a physical process.It was found that the filament or partly network-like structure formed in the modified asphalt system was beneficial to improving the hot storage stability.Moreover,the addition of WPE resulted in a decrease in both the light components volatilization and the macromolecules decomposition of asphalt.It was demonstrated that when the content of WPE in matrix asphalt was less than 10 wt%,the service performances of modified asphalt could be better. | Changqing Fang Ying Zhang Qian Yu Xing Zhou Dagang Guo Ruien Yu Min Zhang | 2013 | Journal of Materials Science & Technology2013，29，5： | 3 |
| 9 | Genome-Wide Identification, Characterization, and Expression Analysis of Calmodulin-Like Proteins(CMLs) in Apple显示文摘Calcium(Ca^(2+)), a crucial second messenger in plants, is involved in diverse signaling pathways including biotic and abiotic stress responses. However, the functions of most calcium sensors including calcium-dependent protein kinases(CDPKs), calmodulins(Ca Ms), calmodulinlike proteins(CMLs), and calcineurin B-like proteins(CBLs) remain limited in plants, particularly in woody plants. Currently, a total of 83 CMLs and seven Ca Ms were discovered in the apple genome in this study. Functional domain analysis showed that these CMLs and Ca Ms contain a highly conserved EF-hand motif. q RT-PCR assays indicated that these CMLs were expressed ubiquitously in apple, including in the roots, stems,leaves, flowers, and fruits, and also possessed spatial specificity. Finally, most of these CMLs were induced by hormonal and abiotic stress, suggesting their potential roles in the regulation of growth, development, and the stress response in apple. In summary, our findings provide potential evidence that apple CMLs may be involved in abiotic stress and the regulation of plant growth and development. | ZHANG Quanyan LIU Xiaojuan LIU Xin WANG Jiahui YU Jianqiang HU Dagang HAO Yujin | 2017 | Horticultural Plant Journal2017，3，6： | 3 |
| 10 | Electrochemical corrosion behavior of steel wires in a coalmine with a corrosive medium显示文摘6 脳升起电报的 19 点接触被用作我们的研究目标在一个实验室检验钢电线的腐蚀的进步,在 coalmine 模仿实际工作条件。一个电气化学的方法被用来与腐蚀剂酸溶液的不同表面处理调查钢电线的腐蚀行为。在当,赤裸的钢电线的阳极激活过程最快的地方,结果证明钢电线的阳极激活主要发生在腐蚀前期间是他们的相应腐蚀速度,当涂的钢电线和他们的相应腐蚀加速的油的阳极激活过程最低时。在中间、迟了的沉浸时期期间,一部被动电影在钢电线的表面上被产生,它逐渐地与时间的推移被损坏。本地 pitting 腐蚀与一个高极化的潜力在钢电线的表面上容易发生。合适的相等的电路被选择在各种各样的腐蚀剂时间和不同表面处理适合钢电线的电气化学的阻抗光谱学(EIS ) ,它显示好恰当的结果。双电的层费用转移抵抗在顺序增加:赤裸的钢电线,未经治疗的钢电线和油涂的钢电线和他们的腐蚀抵抗接着减少,它与他们的极化曲线一致。油层在未经治疗的钢电线上提供某个保护的效果,但是它的效果不是完全清楚的。 | Wang Songquan Zhang Dekun Wang Dagang Zhang Zefeng | 2011 | Mining Science and Technology2011，21，1： | 3 |
| 11 | Enhanced bioactivity and interfacial bonding strength of Ti3Zr2Sn3Mo25Nb alloy through graded porosity and surface bioactivation显示文摘The gradient porous Ti3Zr2Sn3Mo25Nb(TLM)alloy rods were fabricated through sintering the alloyed powder to a solid core.The porous sample was then modified by a Micro Arc Oxidation(MAO)treatment in an electrolyte containing calcium and phosphate,a hydrothermal treatment enabled secondary microporous hydroxyapatite(HA)coating,and a further bone morphogenetic protein-2(BMP-2)loading treatment through immersion and freeze-drying.The treatment led to an orderly secondary microporous coating containing HA nano-particles and evenly distributed BMP-2 in the porous coatings.As a result,osteoblasts could adhere and grow well on the coatings with a high cell adhesion rate and cell functional activity.The in-situ shear testing indicated that the interfacial strength had been enhanced significantly.Improvement of the bond formation and osseointegration with the titanium implant is attributed to increased surface area for the cell to attach,creating voids for the cell to grow in,and activating titanium surface by introducing bioactive ingredients such as HA and BMP-2. | Sen Yu Zhe Yu Dagang Guo Hui Zhu Minghua Zhang Jianye Han Zhentao Yu Yemin Cao Gui Wang | 2022 | Journal of Materials Science & Technology2022，，5： | 3 |
| 12 | Methylesterification of cell-wall pectin controls the diurnal flower-opening times in rice显示文摘Flowers are the core reproductive organ of plants, and flowering is essential for cross-pollination. Diurnal flower-opening time is thus a key trait influencing reproductive isolation, hybrid breeding, and thermostability in plants. However, the molecular mechanisms controlling this trait remain unknown. Here, we report that rice Diurnal Flower Opening Time 1 (DFOT1) modulates pectin methylesterase (PME) activity to regulate pectin methylesterification levels of the lodicule cell walls, which affect lodicule swelling to control diurnal flower-opening time. DFOT1 is specifically expressed in the lodicules, and its expression gradually increases with the approach to flowering but decreases with flowering. Importantly, a knockout of DFOT1 showed earlier diurnal flower opening. We demonstrate that DFOT1 interacts directly with multiple PMEs to promote their activity. Knockout of PME40 also resulted in early diurnal flower opening, whereas overexpression of PME42 delayed diurnal flower opening. Lower PME activity was observed to be associated with higher levels of pectin methylesterification and the softening of cell walls in lodicules, which contribute to the absorption of water by lodicules and cause them to swell, thus promoting early diurnal flower opening. Higher PME activity had the opposite effect. Collectively, our work uncovers a molecular mechanism underlying the regulation of diurnal flower-opening time in rice, which would help reduce the costs of hybrid breeding and improve the heat tolerance of flowering plants by avoiding higher temperatures at anthesis. | Mumei Wang Xiaopei Zhu Guoqing Peng Minglong Liu Shuqing Zhang Minghao Chen Shitang Liao Xiaoying Wei Peng Xu Xiyu Tan Fangping Li Zhichuan Li Li Deng Ziliang Luo Liya Zhu Shuai Zhao Dagang Jiang Jing Li Zhenlan Liu Xianrong Xie Shaokui Wang Aimin Wu Chuxiong Zhuang Hai Zhou | 2022 | Molecular Plant2022，15，6： | 2 |
| 13 | A cell wall-localized NLR confers resistance to Soybean mosaic virus by recognizing viral-encoded cylindrical inclusion protein.显示文摘Soybean mosaic virus (SMV) causes severe yield losses and seed quality reduction in soybean (Glycine max) production worldwide. Rsc4 from cultivar Dabaima is a dominant genetic locus for SMV resistance, and its mapping interval contains three Nucleotide-binding domain Leucine-rich Repeat containing (NLR) candidates (Rsc4-1, Rsc4-2, and Rsc4-3). The NLR-type resistant proteins were considered as important intracellular pathogen sensors in the previous studies. In this research, based on transient expression assay in Nicotiana benthamiana leaves, we found that the longest transcript of Rsc4-3 is sufficient to induce resistance response to SMV;and CRISPR/Cas9-mediated Rsc4-3 knockout in resistant cultivar Dabaima compromised the resistance. These indicate that Rsc4-3 confers resistance to SMV. Interestingly, Rsc4-3 encodes a cell wall localized NLR-type resistant protein (Rsc4-3). The internal polypeptide region responsible for apoplastic targeting of Rsc4-3 and the putative palmitoylation sites on the N-terminus are essential for the resistance response. Furthermore, we showed that viral-encoded cylindrical inclusion (CI) protein partially localizes to the cell wall and can interact with Rsc4-3. Virus-driven or transient expression of CI protein of avirulent SMV strains is enough to induce resistance response in the presence of Rsc4-3, suggesting that CI is the avirulent gene for Rsc4-3 mediated resistance. Our work exhibited a case of NLR recognizing virus in the apoplast and provided a simple and effective method for identifying resistant genes against SMV infection. | Jinlong Yin Liqun Wang Tongtong Jin Yang Nie Hui Liu Yanglin Qiu Yunhua Yang Bowen Li Jiaojiao Zhang Dagang Wang Kai Li Kai Xu Haijian Zhi | 2021 | Molecular Plant2021，14，11： | 2 |
| 14 | Effect of various kinematic parameters of mine hoist on fretting parameters of hoisting rope and a new fretting fatigue test apparatus of steel wires显示文摘 | Dagang Wang Dekun Zhang Zefeng Zhang Shirong Ge | 2012 | Engineering Failure Analysis2012，，： | 2 |
| 15 | Floating Production Platforms and their Applications in the Development of Oil and Gas Fields in the South China Sea显示文摘This paper studies the current available options for floating production platforms in developing deepwater oil fields and the potential development models of future oil and gas exploration in the South China Sea. A detailed review of current deepwater platforms worldwide was performed through the examples of industry projects, and the pros and cons of each platform are discussed. Four types of platforms are currently used for the deepwater development: tension leg platform, Spar, semi-submersible platform, and the floating production system offloading. Among these, the TLP and Spar can be used for dry tree applications, and have gained popularity in recent years. The dry tree application enables the extension of the drilling application for fixed platforms into floating systems, and greatly reduces the cost and complexity of the subsea operation. Newly built wet tree semi-submersible production platforms for ultra deepwater are also getting their application, mainly due to the much needed payload for deepwater making the conversion of the old drilling semi-submersible platforms impossible. These platforms have been used in different fields around the world for different environments; each has its own advantages and disadvantages. There are many challenges with the successful use of these floating platforms. A lot of lessons have been learned and extensive experience accumulated through the many project applications. Key technologies are being reviewed for the successful use of floating platforms for field development, and potential future development needs are being discussed. Some of the technologies and experience of platform applications can be well used for the development of the South China Sea oil and gas field. | Dagang Zhang Yongjun Chen Tianyu Zhang | 2014 | Journal of Marine Science and Application2014，13，1： | 2 |
| 16 | Temporal and spatial variations of net primary productivity and its response to groundwater of a typical oasis in the Tarim Basin, China显示文摘Net primary productivity (NPP) of the vegetation in an oasis can reflect the productivity capacity of a plant community under natural environmental conditions. Owing to the extreme arid climate conditions and scarce precipitation in the arid oasis regions, groundwater plays a key role in restricting the development of the vegetation. The Qira Oasis is located on the southern margin of the Taklimakan Desert (Tarim Basin, China) that is one of the most vulnerable regions regarding vegetation growth and water scarcity in the world. Based on remote sensing images of the Qira Oasis and daily meteorological data measured by the ground stations during the period 2006-2019, this study analyzed the temporal and spatial patterns of NPP in the oasis as well as its relation with the variation of groundwater depth using a modified Carnegie Ames Stanford Approach (CASA) model. At the spatial scale, NPP of the vegetation decreased from the interior of the Qira Oasis to the margin;at the temporal scale, NPP of the vegetation in the oasis fluctuated significantly (ranging from 29.80 to 50.07 g C/(m2•month)) but generally showed an increasing trend, with the average increase rate of 0.07 g C/(m2•month). The regions with decreasing NPP occupied 64% of the total area of the oasis. During the study period, NPP of both farmland and grassland showed an increasing trend, while that of forest showed a decreasing trend. The depth of groundwater was deep in the south of the oasis and shallow in the north, showing a gradual increasing trend from south to north. Groundwater, as one of the key factors in the surface change and evolution of the arid oasis, determines the succession direction of the vegetation in the Qira Oasis. With the increase of groundwater depth, grassland coverage and vegetation NPP decreased. During the period 2008-2015, with the recovery of groundwater level, NPP values of all types of vegetation with different coverages increased. This study will provide a scientific basis for the rational utilization and sustainable management of groundwater resources in the oasis. | SUN Lingxiao YU Yang GAO Yuting ZHANG Haiyan YU Xiang HE Jing WANG Dagang Ireneusz MALIK Malgorzata WISTUBA YU Ruide | 2021 | Journal of Arid Land2021，13，11： | 2 |
| 17 | Structure and properties of soy protein films plasticized with hydroxylamine显示文摘 | Huafeng Tian Dagang Liu Lina Zhang | 2009 | Journal of Applied Polymer Science2009，111，3： | 1 |
| 18 | An analytical solution to steady-state temperature distribution of N-layer viscoelastic suspensions used in crawler vehicles显示文摘 | Song Yong Sun Dagang Zhang Xin | 2012 | International Journal of Heavy Vehicle Systems2012，19，3： | 1 |
| 19 | Reliability analysis of voting engineering systems with intermediate state before failure显示文摘 | Zhang Peng Lu Dagang Wang Guangyuan | 2000 | Advances in Systems Science and Applications2000，1，2： | 1 |
| 20 | An introduction to hull design practices for deepwater floating structures 显示文摘 | Zhang Dagang Deng Zhangchao Yan Fasuo | 2009 | Journal of Marine Science and Application2009，，： | 1 |
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