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| 1 | Dual-specificity histone demethylase KIAA1718 (KDM7A) regulates neural differentiation through FGF4显示文摘histone H3 离氨酸的 Dimethylations 9 和离氨酸 27 是与抄写压抑联系的重要 epigenetic 标记。这里,我们作为为这二个镇压标记特定的新奇 histone demethylase 识别了 KIAA1718 (KDM7A ) 。用老鼠胚胎的干细胞,我们证明那 KIAA1718 表情在神经区别的早阶段增加了。基因击倒堵住的神经区别和效果被野类型的人的基因,并且不由催化地不活跃的异种救。另外, KIAA1718 的 overexpression 加速了神经区别。我们提供 KDM7A 的支持 neural 区别效果通过 FGF4 的直接 transcriptional 激活被调停的证据,一个信号分子在神经区别含有。因此,我们的学习识别了通过 FGF4 调整神经区别的双特性的 histone demethylase。 | Chengyang Huang Yang Xiang Yanru Wang Xia Li Longyong Xu Ziqi Zhu Ting Zhang Qingqing Zhu Kejing Zhang Naihe Jing Charlie Degui Chen | 2010 | Cell Research2010,20,2: | 15 |
| 2 | PHF8 is a histone H3K9me2 demethylase regulating rRNA synthesis显示文摘histone H3 离氨酸 9 的 Dimethylation (H3K9me2 ) 是与抄写压抑联系的一个重要 epigenetic 标记。这里,我们识别了 PHF8, JmjC-domain-containing 蛋白质,为这个镇压标记特定的 histone demethylase。Recombinant 全身的野类型蛋白质能把 methylation 从 H3K9me2 移开,但是到丙氨酸 H247A 的保存 histidine 的变化废除 demethylase 活动。Overexpressed 外长的 PHF8 是 colocalized, B23 染色。内长的 PHF8 也是有 B23 和 fibrillarin 的 colocalized,二生长得很好的核蛋白质,建议那 PHF8 在核是局部性的并且可以调整 rRNA 抄写。确实, PHF8 跳了到 rDNA 基因的倡导者区域。减少的 PHF8 击倒 rRNA 的表示,和基因的 overexpression 导致了 rRNA 的 upregulation 抄本。附随地, H3K9me2 水平在 PHF8 击倒的房间在 rDNA 基因的倡导者区域被提高并且当野类型然而并非催化地不活跃的 H247A 变异的 PHF8 是 overexpressed 时,显著地减少了。因此,我们的学习为调整 rRNA 抄写的 H3K9me2 识别了 histone demethylase。 | Ziqi Zhu Yanru Wang Xia Li Yiqin Wang Longyong Xu Xiang Wang Tianliang Sun Xiaobin Dong Lulu Chen Hailei Mao Yi Yu Jingsong Li Pin Adele Chen Charlie Degui Chen | 2010 | Cell Research2010,20,7: | 7 |
| 3 | A sequence of 28S rRNA-derived small RNAs is enriched in mature sperm and various somatic tissues and possibly associates with inflammation显示文摘 | Chu, Chen Yu, Lu Wu, Bin Ma, Li Gou, Lan-Tao He, Miao Guo, Yunli Li, Zhi-Tong Gao, Wei Shi, Huijuan Liu, Mo-Fang Wang, Hongyan Chen, Charlie Degui Drevet, Joel R. Zhou, Yuchuan Zhang, Yonglian | 2017 | Journal of Molecular Cell Biology2017,9,3: | 6 |
| 4 | The histone H3 lysine-27 demethylase Jmjd3 plays a critical role in specific regulation of Th17 cell differentiation显示文摘Interleukin(IL)17-producing T helper(Th17)cells play critical roles in the clearance of extracellular bacteria and fungi as well as the pathogenesis of various autoimmune diseases,such as multiple sclerosis,psoriasis,and ulcerative colitis.Although a global transcriptional regulatory network of Th17 cell differentiation has been mapped recently,the participation of epigenetic modifications in the differentiation process has yet to be elucidated.We demonstrated here that histone H3 lysine-27(H3K27)demethylation,predominantly mediated by the H3K27 demethylase Jmjd3,crucially regulated Th17 cell differentiation.Activation of naı¨ve CD41 T cells immediately induced high expression of Jmjd3.Genetic depletion of Jmjd3 in CD41 T cells specifically impaired Th17 cell differentiation both in vitro and in vivo.Ectopic expression of Jmjd3 largely rescued the impaired differentiation of Th17 cells in vitro in Jmjd3-deficientCD41 T cells.Importantly,Jmjd3-deficient mice were resistant to the induction of experimental autoimmune encephalomyelitis(EAE).Furthermore,inhibition of the H3K27 demethylase activity with the specific inhibitor GSK-J4 dramatically suppressed Th17 cell differentiation in vitro.At the molecular level,Jmjd3 directly bound to and reduced the level of H3K27 trimethylation(me3)at the genomic sites ofRorc,which encodes the masterTh17 transcription factorRorgt,and Th17 cytokine genes such as Il17,Il17f,and Il22.Therefore,our studies established acritical role of Jmjd3-mediatedH3K27demethylation inTh17 cell differentiation andsuggest that Jmjd3 can be a novel therapeutic target for suppressing autoimmune responses. | Zhi Liu Wei Cao Longxia Xu Xi Chen Yu Zhan Qian Yang Sanhong Liu Pengfei Chen Yuhang Jiang Xiaohua Sun Yu Tao Yiming Hu Cuifeng Li Qi Wang Ying Wang Charlie Degui Chen Yufang Shi Xiaoren Zhang | 2015 | Journal of Molecular Cell Biology2015,7,6: | 6 |
| 5 | PhieCBEs:Plant High-Efficiency Cytidine Base Editors with Expanded Target Range显示文摘Clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated protein 9(Cas9)-mediated genome editing can efficiently produce gene-knockout mutants.On the other hand,CRISPR/Cas-derived base editors offer the ability to induce precise nucleotide substitutions(Komor et al.,2016).Cytidine base editors(CBEs)consist of a cytidine deaminase fused with a Cas9-nickase variant(Cas9n,with a D10A substitu-tion)and can achieve site-specific C-to-T substitution.Similarly,adenine base editors use an adenine deaminase forA-to-G substi-tution.These systems have been used in various organisms(Mishra et al.,2019).However,the Cas9 complex requires target sites containing NGG protospacer adjacent motifs(PAMs),thus restricting selection of potential targets.A number of CBEs have been developed using Cas9 variants(mostly Cas9n),cytidine deaminases(such as rAPOBEC1 and PmCDA1),and uracil glycosylase inhibitor(UGI)domains.These CBEs of the first generation(BE1,rAPOBEC1-dCas9),second generation(BE2,rAPOBEC1-dCas9-UGI),and third generation(BE3,rAPOBEC1-Cas9n-UGI)have moderate editing efficiencies in mammalians(Komor etal.,2016). | Dongchang Zeng Taoli Liu Jiantao Tan Yaling Zhang Zhiye Zheng Bin Wang Degui Zhou Xianrong Xie Menghui Guo Yao-Guang Liu Qinlong Zhu | 2020 | Molecular Plant2020,13,12: | 5 |
| 6 | Structural insights into a dual-specificity histone demethylase ceKDM7A from Caenorhabditis elegans显示文摘Histone 离氨酸 methylation 能被 JmjC 包含域的蛋白质在 sequence-state-specific 和 methylation-state-specific 举止移开。然而,底层特性怎么样,决定,酶怎么被调整,大部分是未知的。我们最近发现了那 ceKDM7A,一个哲学博士 -- 并且 JmjC 包含域的蛋白质,是为 H3K9me2 特定的 histone demethylase, H3K27me2,和 PHD 摸绑定到 H3K4me3 指南在 vivo 的 demethylation 活动。为了为酶的活动和 PHD 的功能提供结构的卓见进分子的机制,摸,我们与包含 H3K4me3, H3K9me2,和 H3K27me2 修正的各种各样的联合的单身者或二肽一起在 apo 形式并且在建筑群解决了酶的六水晶结构。结构显示 H3K9me2 和 H3K27me2 以一种类似的方式与 ceKDM7A 交往,并且肽绑定特性被特定的相互作用的一个网络决定。结构的几何测量也揭示了与 PHD 手指和 H3K9me2 界限联系到 JmjC 领域的那 H3K4me3 从二个分开的分子,建议 trans-histone 肽绑定机制。因此,我们的全身的结构的研究重要地由催化领域而且更多揭示底层识别不仅,为 H3K9me2 和 H3K27me2 的 ceDKM7A 的双特性的分子的机制。 | Ying Yang Lulu Hu Ping Wang Haifeng Hou Yan Lin Yi Liu Ze Li Rui Gong Xiang Feng Lu Zhou Wen Zhang Yuhui Dong Huirong Yang Hanqing Lin Yiqin Wang Charlie Degui Chen Yanhui Xu | 2010 | Cell Research2010,20,8: | 3 |
| 7 | Coordinated regulation of active and repressive histone methylations by a dual-specificity histone demethylase ceKDM7A from Caenorhabditis elegans显示文摘H3K9me2 和 H3K27me2 是与抄写压抑联系的重要 epigenetic 标记,当 H3K4me3 与抄写激活被联系时。活跃、压抑的 histone methylations 以一种互相独占的方式散布,这被显示出,但是内在的机制糟糕被理解。这里,我们识别了 ceKDM7A,一个哲学博士(植物 homeodomain )- 并且 JmjC 包含域的蛋白质,为 H3K9me2 和 H3K27me2 特定的 histone demethylase。我们进一步证明 ceKDM7A 的 PHD 领域在染色体宽的水平绑了 H3K4me3 和与 ceKDM7A co 局部性的 H3K4me3。到 H3K4me3 的 PHD 域绑定的混乱在 vivo 减少了 demethylase 活动,并且 ceKDM7A 的损失减少了它的联系目标基因的表达式。这些结果显示 ceKDM7A 被招募到倡导者到 demethylate H3K9me2 和 H3K27me2 并且通过 PHD 领域的绑定激活基因表示到 H3K4me3。因此,我们的学习识别双特性的 histone demethylase 并且提供新奇卓见进 histone methylation 的规定。 | Hanqing Lin Yiqin Wang Yanru Wang Feng Tian Pu Pu Yi Yu Hailei Mao Ying Yang Ping Wang Lulu Hu Yan Lin Yi Liu Yanhui Xu Charlie Degui Chen | 2010 | Cell Research2010,20,8: | 2 |
| 8 | Genetic and Phenotypic Investigation of a Chinese Pedigree with Lattice Corneal Dystrophy ⅢB Subtype显示文摘Purpose:.To investigate phenotypes and disease-causing mutation in the transforming growth factor b-induced gene(TGFBI) in a Southern Chinese pedigree with lattice corneal dystrophy(LCD) IIIB with complicated cataract.Methods:.A Southern Chinese pedigree with lattice corneal dystrophy IIIB with complicated cataract was recruited. Comprehensive ophthalmic investigations were performed before and after cataract surgery of phacoemulsification and intraocular lens implantation in the proband's both eyes..Peripheral blood was collected from the proband,.and genomic DNA was extracted..All exons of the TGFBI gene were sequenced to screen possible mutations.Results:.A bilateral LCD IIIB subtype was observed in the proband..Optical coherence tomography further revealed superreflective changes in the subepithelial and stroma layers of the cornea,.with reduced central corneal thickness..Notably,bilateral cataract was found in the proband..Direct sequencing detected a recurrent heterozygous missense c.1877A>G mutation in exon 14 of the TGFBI gene,.resulting in substitution of histidine with arginine(p.H626R).Conclusion:.The current study was the first report of the TGFBI p.H626R mutation in Southern Chinese,.suggesting that it could be a mutation hotspot across populations..Moreover,.the mutation was associated with LCD IIIB subtype with complicated cataract,.which had not been reported before,pointing to clinical heterogeneity of the mutation. | Degui Wang Yong Yao Mingzhi Zhang Jianhuan Chen | 2013 | Eye Science2013,28,3: | 1 |
| 9 | Effects of curcumin on bladder cancer cells and development of urothelial tumors in a rat bladder carcinogenesis model显示文摘 | Binqiang Tian Zhiping Wang Yingmei Zhao Degui Wang Yonggang Li Li Ma Xiaoming Li Jing Li Nan Xiao Junqiang Tian Ronald Rodriguez | 2008 | Cancer Letters2008,,2: | 1 |
| 10 | Two hour exposure to sodium butyrate sensitizes bladder cancer to antieaneer drugs 显示文摘 | Degui Wang Zhiping Wang Binqiang Tian | 2008 | Int J Urol2008,15,5: | 1 |
| 11 | A new panel containing specific spermatogenesis markers to identify spermatogenic cells in nonobstructive azoospermia patients显示文摘Infertility is a severe problem that affects 10%-15% of couples worldwide, among which male infertility is a factor in ~50% of the cases [1]. Azoospermia, which is defined as the absence of sperm in at least two ejaculates, can be divided into two types: obstructive azoospermia (OA) and non-OA (NOA)[1]. OA patients have normal spermatogenesis and mature sperm in testes and can father a biological child through testicular sperm retrieval and intracytoplasmic sperm injection (ICSI)[2]. By contrast, NOA patients usually have various degrees of spermatogenesis impairment and can be faced with complicated assisted reproductive technology (ART) strategies to father a biological child [2]. The precise diagnosis of spermatogenesis in NOA patients is crucial for clinical decision making regarding ART. | Xinxi Zhao Yiqin Wang Meng Ma Wei Zhang Jiqiang Si Wei Wang Zhen Lin Gang Han Charlie Degui Chen Minghan Tong Yanping Kuang Qifeng Lyu | 2019 | Acta Biochimica et Biophysica Sinica2019,51,6: | 1 |
| 12 | Expression and significance of PTEN in canine mammary gland tumours显示文摘 | Qiu Changwei Lin Degui Wang Jinqiu | 2008 | Res Vet Sci2008,,: | 1 |
| 13 | Comparisons of Metabolic Profiles for Carbohydrates,Amino Acids,Lipids,Fragrance and Flavones During Grain Development in indica Rice Cultivars显示文摘We used a widely targeted metabolomics approach to examine the metabolic changes in three pedigreed indica cultivars(Meixiangzhan 2,Meisizhan and Qixinzhan),with different eating qualities,at 8,15 and 30 d after flowering(DAF)to explore the formation mechanism of rice eating and nutritional qualities at a global metabolic level.A total of 623 metabolites were identified,and results showed that metabolic variations among rice cultivars decreased with grain developmental stage,suggesting that sufficient carbohydrate and amino acid supply during grain development may contribute to excellent rice eating and nutritional quality formation.Lysophosphatidylcholines 19:0 and 16:1 were beneficial for excellent eating quality formation during grain development.Rice fragrance was attributed mainly to spermidine andγ-aminobutyric acid.Rice cultivars with excellent eating quality at 15-30 DAF had relatively higher flavone content,possibly because they had adequate carbohydrate and amino acid contents during grain development.These results provided insight into the mechanisms for establishing rice eating and nutritional qualities during grain formation at a global metabolic level,and can be applied towards improving rice quality. | CHEN Yibo WANG Zhidong WANG Chongrong LI Hong HUANG Daoqiang ZHOU Degui ZHAO Lei PAN Yangyang GONG Rong ZHOU Shaochuan | 2022 | Rice science2022,29,2: | 0 |
| 14 | RAD6B participates in neuronal DNA damage response to resist neurodegeneration显示文摘Protein ubiquitination plays an important role in DNA damage repair.To clarify the role of RAD6B,identified as an important ubiquitin conjugating enzyme,in neuronal genome integrity and survival,we first silenced RAD6B gene in RPE1 and found that RAD6B deficiency resulted in increased genomic instability.While in brains of RAD6B knockout mouse generated by homologous recom bination,we found that RAD6B deficiency caused a significant reduction of H2B ubiquitination and impaird DNA damage repair compared with wild type mouse brains.The accumulation of unrepaired DNA damage often leads to apoptosis or senescence,even tumor formation.Furthermore,we evaluated the effect of RAD6B deficiency on the survival of neurons,and noticed that RAD6B-deficient mice exhibited marked neuronal senescence,neurodegeneration and reduced neuronal numbers.We also observed learning and memory impairment in neuron-specific RAD6B-deficient mice.In conclusion,RAD6B is involved in DNA damage repair of neurons,and is essential to maintain neuronal genome stability. | Guo Zhao Tian Yingxia Guo Yingli Li Boya Liu Xiangwen Xie Kun Song Yanfeng Wang Degui | 2021 | 解剖学杂志2021,44,S01: | 0 |
| 15 | Exponentially reduced carrier mobility of natural ester via blocking effect of 2D hexagonal boron nitride nanosheets显示文摘In this work,the boron nitride(BN)nanosheets were dispersed in natural esters to fabricate the dielectric nanofluid.Microstructures and chemical compositions of the nanosized BN are determined.The stability,viscosity,and thermal conductivity of the BN nanofluid,were obtained.And the dissipation factor,electrical conductivity,and relative permittivity of the BN nanofluid,were measured.The ion mobilities and migrating times of the nanofluid were measured under different volumetric fractions of BN nanosheets and temperatures.Results show that the electrical conductivity and dissipation factor of the nanofluid decreased by 54%and 48%with the addition of only 0.1%of BN nano-sheets under 110°C.Both the half-reduced carrier mobility of the nanofluid and the declined ion concentration by blocking of BN nanosheet contributed to the exponentially reduced electrical conductivity and enhanced dielectric performances of the BN nano-fluid.The suppression coefficient k is proposed to quantitatively describe the hinder effect of ion migration in nanofluids by 2D BN nanosheet.Results provide a strategy to design and develop advanced nanofluids with low-dielectric loss by aids of surface adsorption of ion and steric hindrance of 2D nanosheets. | Zhengyong Huang Gang He Jian Li Feipeng Wang Ruiqi Zhang Degui Yao | 2021 | High Voltage2021,6,2: | 0 |
| 16 | R&D of BEPCII 500 MHz superconducting cavity显示文摘Beijing Electron-Positron Collider Upgrade (BEPCII) adopts two 500 MHz superconducting cavities (SCCs) in each ring for higher accelerated gradient, higher Q and lower impedance (Wang et al. The proceedings of SRF'07). There's no spare cavity due to the limited time and funding during BEPCII construction. If any serious trouble happened on either one of the two cavities and could not be recovered in a short time, the operation of BEPCII facility will be affected. Therefore, since 2009 three spare cavities have been fabricated in China to ensure reliable operation, and two of them have been successfully vertically tested in January and July 2011. This paper will briefly present the manufacture, post-process and vertical test performance of the 500 MHz spare cavities. | LIU YaPing WANG GuangWei PAN WeiMin LI JiZhen LIU DeGui SUN Yi LI ZhongQuan DAI JianPing LI ShaoPeng HE Kun WANG GuoPing ZHAO GuangYuan MA Qiang LIN HaiYing SHA Peng WANG QunYao QIU Feng MENG FanBo LI Han | 2011 | Science China(Physics,Mechanics & Astronomy)2011,54,S2: | 0 |
| 17 | δ13C-δ18O Covariance:An Effective Indicator of Hydrological Closure for Lakes?显示文摘在 13 C 之间的关联并且 18 0 被象水文学平衡,全部的 CO2 集中,气候的条件和湖生产率那样的几个因素在主要碳酸盐影响。13 C-18 0 关联上的这些因素的影响可能在不同时间规模上是不同的。在这份报纸,在西南的中国的二个不同类型的湖,厄海湖和琴海湖,被选择在 13 C-18 0 关联上调查气候的模式的影响并且作为水文学闭合的指示物评估 13 C-18 0 协变性的可靠性。结果证明那在那里存在在 13 C 之间的好关联并且 18 0 在厄海湖(溢出的开的湖) 并且在琴海湖(关上的湖) 。这建议 13 C-18 0 协变性可以不是为湖的水文学闭合的有效指示物,特别在短时间规模上。在一方面,一个 hydrologically 开的湖可以显示 covariant 13 C 并且 18 0 由于气候的影响。在西南的中国的特别交替的温暖干燥、寒冷湿的气候的模式可以是在厄海湖和琴海湖的 13 C-18 0 协变性的主要原因。在另一方面,因为高 CO2 集中的缓冲效果,一个 hydrologically 关上的湖不必要地在超湖在 13 C 移动上显示在 13 C 和 18 O 之间的 covariant 趋势。当我们使用在 13 C 和 18 O 之间的协变性判定湖的水文学闭合时,我们应该是谨慎的。 | CHEN Jing'an WANG Fushun WAN Guojiang TANG Degui David Dian ZHANG HUNAG Ronggui LI Jian XIAO Tangfu | 2008 | Acta Geologica Sinica(English Edition)2008,82,5: | 0 |
| 18 | Calculation of the Gas Injection Rate and Pipe String Erosion in Nitrogen Drilling Systems显示文摘Detailed information is provided for the design and construction of nitrogen drilling in a coal seam.Two prototype wells are considered.The Guo model is used to calculate the required minimum gas injection rate,while the Finnie,Sommerfeld,and Tulsa models are exploited to estimate the ensuing erosion occurring in pipe strings.The calculated minimum gas injection rates are 67.4 m^(3)/min(with water)and 49.4 m^(3)/min(without water),and the actual field of use is 90–120 m^(3)/min.The difference between the calculated injection pressure and the field value is 6.5%–15.2%(formation with water)and 0.65%–7.32%(formation without water).The results show that the Guo model can more precisely represent the situation of the no water formation in the nitrogen drilling of a coal seam.The Finnie,Sommerfeld,and Tulsa models have different sensitivities to cutting densities,particle size,impact velocity and angle,and pipe string hardness. | Mingren Shao Chunpeng Wang Degui Wang Wenbo Mei Mingjie Li Hao Yang | 2022 | Fluid Dynamics & Materials Processing2022,18,2: | 0 |
| 19 | Histone post-translational modification and the DNA damage response显示文摘DNA is highly vulnerable to spontaneous and environmental timely damage in living cells.DNA damage may cause genetic instability and increase cancer risk if the damages are not repaired timely and efficiently.Human cells possess several DNA damage response(DDR)mechanisms to protect the integrity of their genome.Clarification of the mechanisms under-lying the DNA damage response following lethal damage will facilitate the identification of therapeutic targets for cancers.Histone post-translational modifications(PTMs)have been indicated to play different roles in the repair of DNA damage.In this context,histone PTMs regulate recruitment of downstream effectors,and facilitate appropriate repair response.This review outlines the current understanding of different histone PTMs in response to DNA dam-age repair,besides,enumerates the role of new type PTMs such as histone succinylation and crotonylation in regulating DNA damage repair processes. | Haoyun Song Rong Shen Xiangwen Liu Xuguang Yang Kun Xie Zhao Guo Degui Wang | 2023 | Genes & Diseases2023,10,4: | 0 |
| 20 | Genomic prediction of yield performance among single-cross maize hybrids using a partial diallel cross design显示文摘Genomic prediction(GP)in plant breeding has the potential to predict and identify the best-performing hybrids based on the genotypes of their parental lines.In a GP experiment,34 elite inbred lines were selected to make 285 single-cross hybrids in a partial-diallel cross design.These lines represented a mini-core collection of Chinese maize germplasm and comprised 18 inbred lines from the Stiff Stalk heterotic group and 16 inbred lines from the Non-Stiff Stalk heterotic group.The parents were genotyped by sequencing and the 285 hybrids were phenotyped for nine yield and yield-related traits at two locations in the summer sowing area(SUS)and three locations in the spring sowing area(SPS)in the main maizeproducing regions of China.Multiple GP models were employed to assess the accuracy of trait prediction in the hybrids.By ten-fold cross-validation,the prediction accuracies of yield performance of the hybrids estimated by the genomic best linear unbiased prediction(GBLUP)model in SUS and SPS were 0.51 and 0.46,respectively.The prediction accuracies of the remaining yield-related traits estimated with GBLUP ranged from 0.49 to 0.86 and from 0.53 to 0.89 in SUS and SPS,respectively.When additive,dominance,epistasis effects,genotype-by-environment interaction,and multi-trait effects were incorporated into the prediction model,the prediction accuracy of hybrid yield performance was improved.The ratio of training to testing population and size of training population optimal for yield prediction were determined.Multiple prediction models can improve prediction accuracy in hybrid breeding. | Ping Luo Houwen Wang Zhiyong Ni Ruisi Yang Fei Wang Hongjun Yong Lin Zhang Zhiqiang Zhou Wei Song Mingshun Li Jie Yang Jianfeng Weng Zhaodong Meng Degui Zhang Jienan Han Yong Chen Runze Zhang Liwei Wang Meng Zhao Wenwei Gao Xiaoyu Chen Wenjie Li Zhuanfang Hao Junjie Fu Xuecai Zhang Xinhai Li | 2023 | The Crop Journal2023,11,6: | 0 |