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| 1 | Pediatric reference intervals in China(PRINCE):design and rationale for a large,multicenter collaborative cross-sectional study显示文摘There is a lack of accurate pediatric reference intervals(RIs) in China, with most commonly used RIs established without consideration of the effect of age and gender. The Pediatric Reference Intervals in China(PRINCE) project aims to establish and verify pediatric RIs for 31 common laboratory measurands.The project will be a large, multicenter cross-sectional study:14,490 healthy children and adolescents aged up to 19 years will be surveyed by 10 children's hospitals and one pediatric department of a university hospital. To evaluate the feasibility and efficiency of the study methods, 602 children were surveyed in the pilot phase of the PRINCE study in April 2017: it found that some measurands were distinctly age dependent and that there were differences between values for males and females. The results of the pilot study affirmed the necessity of the PRINCE project for Chinese pediatrics. The pilot also indicated potential difficulties in the full survey, e.g., difficulties in recruiting children aged under 3 years and insufficient collection of blood samples from infants. The operation of the PRINCE project has been modified based on the findings in the pilot study toward improving the validity of the PRINCE project and promoting its openness and transparency. | Xin Ni Wenqi Song Xiaoxia Peng Ying Shen Yaguang Peng Qiliang Li Yan Wang Lixin Hu Yanying Cai Hong Shang Min Zhao Hong Jiang Yaoguo Huang Runqing Mu Wenxiang Chen Mingting Peng Chuanbao Zhang Jie Zeng Chenbin Li Hongling Yang Yongmei Jiang Jin Xu Guixia Li Hongbing Chen Yun Xiang Sancheng Cao Zhenxin Guo Dapeng Chen | 2018 | Science Bulletin2018，63，24： | 12 |
| 2 | Prognostic value of programmed death.1, programmed death-ligand 1, programmed death-ligand 2 expression, and CD8(+) T cell density in primary tumors and metastatic lymph nodes from patients with stage T1.4N+M0 gastric adenocarcinoma显示文摘Background: Anti-programmed death-1/programmed death-ligand 1(PD-1/PD-L1) immunotherapy has been proved to be effective on gastric cancer in ongoing clinical trials. However, the value of PD-L1 in predicting responses of patients with gastric cancer to anti-PD-1/PD-L1 immunotherapy is controversial. Some studies suggested that intra-and inter-tumoral heterogeneity of PD-L1 expression might explain the controversy.This study aimed to analyze the expression of PD-L1, PD-L2, and PD-1 as well as CD8(+) T-cell density in primary tumors and lymph nodes from patients with stage T1-4 N+M0 gastric adenocarcinoma to explore the heterogeneity of PD-1 signaling pathway molecules.Methods: In primary tumors and metastatic as well as non-metastatic lymph nodes from patients with stage T1-4 N+M0 gastric adenocarcinoma, we detected PD-L1 and PD-L2 expression with immunohistochemistry. CD8(+)T-cell density in primary tumors and PD-1 expression on CD8(+)T cells were detected with immunofluorescence. Univariate analysis was used to determine the prognostic values of them. Cox proportional hazard regression model was used to identify independent risk factors that affect patients' overall survival and disease-free survival.Results: Among 119 eligible patients who had undergone surgical resection, the positive rate of PD-L1 was higher in metastatic lymph nodes than in primary tumors(45.4% vs. 38.7%, P = 0.005); the positive rate of PD-1 on CD8(+)T cells was significantly higher in primary tumors and metastatic lymph nodes than in tumor-free lymph nodes(both P < 0.001). The intensity of PD-1 expression on CD8(+) T cells in primary tumors and in metastatic lymph nodes were stronger than that in tumor-free lymph nodes from the same patient. Beside, the positive rate of PD-L2 did not show any differences between primary tumors and metastatic lymph nodes. In multivariate analysis, PD-L1 expression,PD-L2 expression, a low density of CD8(+) T cells in primary tumors, and PD-1 expression on CD8(+) T cells in primary tumors were associated with poor prognosis.Conclusion: The expression of PD-L1 is heterogeneous in primary tumors and in metastatic lymph nodes from patients with stageT1-4 N+M0 gastric adenocarcinoma, which might explain the inconsistent results in assessing the prognostic value of PD-L1 expression in previous studies. | Yuan Gao Su Li Dazhi Xu Shangxiang Chen Yuchen Cai Wenqi Jiang Xinke Zhang Jin Sun Kefeng Wang Boyang Chang Fenghua Wang Minghuang Hong | 2017 | Chinese Journal of Cancer2017，36，11： | 8 |
| 3 | Trot- ting Gait of a Quadruped Robot Based on the Time- pose Control Method显示文摘 | Cai Runbin Chen Yangzhen Hou Wenqi | 2013 | International Journal of Advanced Robotic Systems2013，10，148： | 1 |
| 4 | Laparoscopic pancreatico - duodenectomy to treat cancer of the Ampulla of Vater显示文摘 | Bangyu Lu Xiaoyong Cai Wenqi Lu | 2006 | JSLS2006，，10： | 1 |
| 5 | Diarylquinoline compounds induce autophagy-associated cell death byinhibiting the Akt pathway and increasing reactive oxygen species in human nasopharyngealcarcinoma cells显示文摘 | Yuchen Cai Zhihui Wan Tiemin Sun Yanxia Shi Yueli Sun Peiyu Huang Su Li Wenqi Jiang | 2013 | Oncology Reports2013，，3： | 1 |
| 6 | Genotyping of IL-8-251 T > A yields prognostic information in patients with gastric carcinoma显示文摘 | Xiuyu Cai Weihan Hu Bei Zhang Ni Dai Ruihua Xu Huijuan Qiu Deshen Wang Zhiming Li Wenqi Jiang | 2013 | Biomarkers2013，，7： | 1 |
| 7 | Application of ERA5 Reanalysis to the Construction of Initial Conditions for WACCM Simulations显示文摘This study uses ECMWF fifth-generation reanalysis, ERA5, which extends to the mesopause, to construct the Initial Conditions(IC) for WACCM(Whole Atmosphere Community Climate Model) simulations. Because the biases between ERA5 and Sounding of the Atmosphere using Broadband Emission Radiometry(SABER) temperature data are within ±5 K below the lower mesosphere,ERA5 reanalysis is used to construct IC in the lower atmosphere. Four experiments are performed to simulate a Stratospheric Sudden Warming(SSW) event from 5 to 15 February 2016. The simulation using the WACCM default climatic IC cannot represent the sharp meteorological variation during SSW. In contrast, the 0~4 d forecast results driven by ERA5-constructed IC is consistent with ERA5 reanalysis below the middle mesosphere. Comparing with WACCM climatology ICs scheme, the ICs constructing method based on ERA5 reanalysis can obtain 67%, 40%, 22%, 4% and 6% reduction of temperature forecast RMSE at 10 hPa, 1 hPa, 0.1 hPa, 0.01 hPa and 0.001 hPa respectively. However,such improvement is not shown in the lower thermosphere. | WANG Yegui JING Wenqi CUI Yuanyuan CAI Qifa LAN Weiren FANG Hanxian WENG Libin NIU Jun | 2018 | 空间科学学报2018，38，4： | 1 |
| 8 | Trotting gait of a quadruped robot based on the time- pose control method 显示文摘 | Cai Runbin Chen Yangzhen Hou Wenqi | 2013 | International Journal of Advanced Robotic Systems2013，10，148： | 1 |
| 9 | A novel method for reconstruction in laparoscopic pancreaticoduodenectomy:an experience of 13 cases显示文摘Laparoscopic pancreaticoduodenectomy(LPD)is a challenging operation to general surgeon.Up to date,only about 135 cases have been reported,16 cases in China,119 cases outside China.The reconstruction of alimentary system is a key procedure to ensure success of the whole surgery.It is worth investigating the methods of reconstruc-tion in LPD.A retrospective study is made to investigate the methods of reconstruction in LPD.We analyze 13 cases of LPD performed in our center.Child’s or modified Child’s method was used to make the reconstruction in our practice.We tried three methods to make the anastomosis of pancre-aticojejunostomy,including end-to-end dunking binding pancreaticojejunostomy in two cases,end-to-end dunking pancreaticojejunostomy using interrupted suture in two cases,and duct-to-jejunal end-to-side embedding pancreaticojeju-nostomy in nine cases.The clinical data was collected and analyzed.Three of four patients,who underwent end-to-end pancreaticojejunostomy,had a little pancreatic leakage,especially in the first case.None of other nine patients,who underwent duct-to-jejunal end-to-side embedding pancreati-cojejunostomy,was detected to have pancreatic leakage,and the operating time of these nine cases was less than other four cases.Duct-to-jejunal end-to-side embedding pancreaticoje-junostomy is a safe and efficient method of reconstruction in LPD. | LU Bangyu HUANG Yubin CAI Xiaoyong HUANG Fei LI Jie LU Wenqi XU Jing LIU Zujun YAN Yihe LI Jianjun | 2007 | Frontiers of Medicine2007，1，4： | 1 |
| 10 | Functional analysis of DNA 4 coding region from a Chinese Zhangzhou isolate of banana bunchy top virus显示文摘The DNA 4 coding region of banana bunchy top virus from a Chinese Zhangzhou isolate (BBTV-ZZ) is cloned by PCR. The sequencing analysis shows that it is 351 nucleotides long and it putatively encodes a protein of 116 amino acids. On the basis of a plant binary vector pBin438, the plant expression vector pBBTV-4B harboring the BBTV-ZZ DNA 4 coding region has been constructed and then transferred to tobacco (Nicotiana tobacum cv. Xanthi nc) by a Agrobacterium-mediated procedure. Under insect-free condition, movement-defective mutant of CMV-Fny strain (CMV-Fny-△MP) is mechanically inoculated on the lower leaves of transgenic plants. Systemic symptoms with different degrees of severity are developed in the upper uninoculated leaves of transgenic plants at 12 days postinoculation (dpi), while no symptoms can be seen in the uninoculated leaves of untransformed plants at any time. Accumulation of CMV-Fny is detected on the upper uninoculated leaves of transgenic plants, but is not on that of untransformed plants by indirect double antibody sandwich enzyme-link immunosorbent assay (DAS-ELISA). The results reveal that transgenic plants have acquired the property of cell-to-cell movement and systemic spread of CMV-Fny-△MP. This suggests that the protein encoded by BBTV-ZZ DNA 4 might have function of viral movement protein. | SUN Dejun, SUN Hui, WEI Hongyan, FAN Xiaoxue, CAI Wenqi and TIAN Yingchuan(Plant Biotechnology Laboratory, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China) | 2002 | Progress in Natural Science:Materials International2002，12，6： | 1 |
| 11 | H3K14me3 genomic distributions and its regulation by KDM4 family demethylases显示文摘 | Bin Zhao Wenqi Xu Bowen Rong Guoyu Chen Xuanjia Ye Ruofei Dai Wenjinq Li Jiajia Chen Jiajun Cai Lei Song Zhao-Qing Luo Rong Zeng Yang Shi Jing-Dong J. Han Fei Lan | 2018 | Cell Research2018，28，11： | 1 |
| 12 | Function of resveratrol derived from transgenic plant expressing resveratrol syn-thase gene显示文摘Two genes from grapevine coding for resvera-trol synthase, named RS1 and RS2, were cloned by RT-PCR. An Escherichia coli expression vector was constructed by insertion of RS1 into pBV221. A specific protein with the same molecular weight (42 ku) as the resveratrol synthase was expressed and used to prepare the rabbit antiserum. A plant expression vector was constructed by inserting the RS1 gene into pBin438 downstream of the doubled CaMV 35S promoter and TMV-Ω fragment. PCR-positive transgenic tobacco plants were obtained after transformation with Agrobacterium tumefaclens LBA4404 harboring the plant expression vector. Southern blot analysis demonstrated that the foreign gene was integrated into the tobacco genome. The results of RT-PCR and Western blot indicated that the RS1 gene was transcribed and expressed. Formation of resveratrol in transgenic tobacco was further determined by thin-layer chromatography of silica gel and HPLC. Increased accumulation of human breast adenocarcinoma cells in G0 and | La Ping Cai Wenqi Zhang Jiuchun Zhang Fengli Fang Rongxiang | 2001 | Chinese Science Bulletin2001，46，13： | 1 |
| 13 | In situ atomic-scale observation of size-dependent (de) potassiation and reversible phase transformation in tetragonal FeSe anodes显示文摘Potassium-ion batteries(PIBs)are considered promising alternatives to lithium-ion batteries owing to cost-effective potassium resources and a suitable redox potential of-2.93 V(vs.-3.04 V for Li+/Li).However,the exploration of appro-priate electrode materials with the correct size for reversibly accommodating large K+ions presents a significant challenge.In addition,the reaction mecha-nisms and origins of enhanced performance remain elusive.Here,tetragonal FeSe nanoflakes of different sizes are designed to serve as an anode for PIBs,and their live and atomic-scale potassiation/depotassiation mechanisms are revealed for the first time through in situ high-resolution transmission electron micros-copy.We found that FeSe undergoes two distinct structural evolutions,sequen-tially characterized by intercalation and conversion reactions,and the initial intercalation behavior is size-dependent.Apparent expansion induced by the intercalation of K+ions is observed in small-sized FeSe nanoflakes,whereas unexpected cracks are formed along the direction of ionic diffusion in large-sized nanoflakes.The significant stress generation and crack extension originating from the combined effect of mechanical and electrochemical interactions are elucidated by geometric phase analysis and finite-element analysis.Despite the different intercalation behaviors,the formed products of Fe and K_(2)Se after full potassiation can be converted back into the original FeSe phase upon depotassiation.In particular,small-sized nanoflakes exhibit better cycling perfor-mance with well-maintained structural integrity.This article presents the first successful demonstration of atomic-scale visualization that can reveal size-dependent potassiation dynamics.Moreover,it provides valuable guidelines for optimizing the dimensions of electrode materials for advanced PIBs. | Ran Cai Lixia Bao Wenqi Zhang Weiwei Xia Chunhao Sun Weikang Dong Xiaoxue Chang Ze Hua Ruiwen Shao Toshio Fukuda Zhefei Sun Haodong Liu Qiaobao Zhang Feng Xu Lixin Dong | 2023 | InfoMat2023，5，1： | 0 |
| 14 | The application of indocyanine green in guiding prostate cancer treatment显示文摘Objective:Indocyanine green(ICG)with near-infrared fluorescence absorption is approved by the United States Food and Drug Administration for clinical applications in angiography,blood flow evaluation,and liver function assessment.It has strong optical absorption in the near-infrared region,where light can penetrate deepest into biological tissue.We sought to review its value in guiding prostate cancer treatment.Methods:All related literature at PubMed from January 2000 to December 2020 were reviewed.Results:Multiple preclinical studies have demonstrated the usefulness of ICG in identifying prostate cancer by using different engineering techniques.Clinical studies have demonstrated the usefulness of ICG in guiding sentinel node dissection during radical prostatectomy,and possible better preservation of neurovascular bundle by identifying landmark prostatic arteries.New techniques such as adding fluorescein in additional to ICG were tested in a limited number of patients with encouraging result.In addition,the use of the ICG was shown to be safe.Even though there are encouraging results,it does not carry sufficient sensitivity and specificity in replacing extended pelvic lymph node dissection during radical prostatectomy.Conclusion:Multiple preclinical and clinical studies have shown the usefulness of ICG in identifying and guiding treatment for prostate cancer.Larger randomized prospective studies are warranted to further test its usefulness and find new modified approaches. | Donghua Xie Di Gu Ming Lei Cong Cai Wen Zhong Defeng Qi Wenqi Wu Guohua Zeng Yongda Liu | 2023 | Asian Journal of Urology2023，10，1： | 0 |
| 15 | Lynch syndrome pre-screening and comprehensive characterization in a multi-center large cohort of Chinese patients with colorectal cancer显示文摘Objective:Lynch syndrome(LS)pre-screening methods remain under-investigated in colorectal cancers(CRCs)in Asia.Here,we aimed to systematically investigate LS pre-screening and comprehensively characterize LS CRCs.Methods:Microsatellite instability(MSI)and germline variants of DNA mismatch repair(MMR)genes were examined in 406 deficient MMR(dMMR)and 250 proficient MMR CRCs.The genetic differences between LS and sporadic CRCs were studied with whole exome sequencing analysis.Results:The incidence of dMMR in Chinese patients with CRCs was 13.8%.Consistency analysis between MMR immunohistochemistry(IHC)and MSI testing showed the kappa value was 0.758.With next-generation sequencing(NGS),germline variants were detected in 154 CRCs.Finally,88 patients with CRC were identified as having LS by Sanger sequencing.Among them,we discovered 21 previously unreported pathogenic germline variants of MMR genes.Chinese patients with LS,compared with sporadic CRCs,tended to be early-onset,right-sided,early-stage and mucinous.Overall,the performance of MMR IHC and MSI testing for LS pre-screening was comparable:the area under the ROC curve for dMMR,MSI-H,and MSI-H/L was 0.725,0.750,and 0.745,respectively.dMMR_MSI-H LS and sporadic CRCs showed substantial differences in somatic genetic characteristics,including different variant frequencies of APC,CREBBP,and KRAS,as well as different enriched pathways of VEGF,Notch,TGFβR,mTOR,ErbB,and Rac protein signal transduction.Conclusions:MMR IHC and MSI testing were effective methods for LS pre-screening.The revealed clinical and somatic genetic characteristics in LS CRCs may have the potential to improve the performance of LS pre-screening in combination with dMMR/MSI. | Yan Li Lihong Fan Jianming Zheng Xiu Nie Yu Sun Qin Feng Shenyi Lian Wenqi Bai Weijing Cai Yanan Yang Bo Su Yanfeng Xi Dongmei Lin | 2022 | Cancer Biology & Medicine2022，19，8： | 0 |
| 16 | COMPENSATION FOR THE MUTUAL COUPLING EFFECT FOR THE ESPRIT ALGORITHM IN SINGLE SNAPSHOT ARRAY PROCESSING显示文摘An effective method is introduced to compensate the effects of mutual coupling for the Estimation of Signal Parameter via Rotational Invariance Techniques (ESPRIT) direction finding algorithm in application of signal snapshot array processing.Changing the covariance matrix into a Teoplitz matrix can achieve high resolution in the Direction Of Arrive (DOA) estimation.How the mutual coupling affects the array antennas has been discussed and a new definition of mutual im- pedance has been used to characterize the mutual coupling effects between the array elements.Based on the new mutual impedance matrix,a practical method is presented to eliminate the effects of mutual coupling for ESPRIT in the single snapshot data processing.The simulation results show that, this new method not only properly reduces the effects of mutual coupling,but also maintains its steady performance even for weak signals. | Lian Xiaohua Zhou Jianjiang Li Hailin Cai Wenqi | 2007 | Journal of Electronics(China)2007，24，5： | 0 |
| 17 | Involvement of the ammonium assimilation mediated by glutamate dehydrogenase in response to heat stress in the scleractinian coral Pocillopora damicornis显示文摘Glutamate dehydrogenase(GDH)plays an important role in the ammonium assimilation and nitrogen metabolism by catalyzing the reversible oxidative deamination of L-glutamate toα-ketoglutarate.In the present study,the potential functions of GDH in response to heat stre ss were explored in the scleractinian coral Pocillopora damicornis(designated as PdGDH).The cDNA of PdGDH contained an open reading frame of 1611 bp encoding a polypeptide of 536 amino acids,which exhibited the highest sequence identity to GDH of Stylophora pistillata(96%identity),and the deduced PdGDH protein was predicted to contain one GdhA domain(from Val95 to Tyr525).The recombinant protein of PdGDH(rPdGDH)was expressed in Escherichia coli BL21(DE3)-Transetta,and its catalytic activity was measured under different temperatures,pH conditions and epigallocatechin-3-gallate(EGCG,a GDH inhibitor)concentrations.The purified rPdGDH only used reduced coenzyme nicotinamide adenine dinucleotide(NADH)as coenzyme,and its highe st activity was observed at 35℃and pH 7.5,re spectively.The rPdGDH activity was negatively correlated with the concentration of EGCG,and was inhibited by more than half(65%,P<0.05)at 10mol/L EGCG.No significant alteration of PdGDH mRNA expression was detected at 12 h after exposure to heat and ammonium(P>0.05).Furthermore,the activities of NADH-GDH in the scleractinian coral P.damicornis increased significantly at 12 h after the heat and ammonium stress,and the NADH-GDH activity in the heat stress group(32.66 U/mg,P<0.05)was significantly higher than that in the heat and ammonium stress group(11.26 U/mg).These results collectively suggested that PdGDH,as a homologue of glutamate dehydrogenase in the scleractinian coral P.damicornis,could respond to heat stress at the protein level,which would have ability to further promote ammonium assimilation to increase the heat acclimatization of the coral-Symbiodiniaceae symbiotic association. | Jia TANG Wenqi CAI Zhicong YAN Zhongjie WU Qianxi YANG Zhi ZHOU | 2022 | Journal of Oceanology and Limnology2022，40，5： | 0 |
| 18 | VAMP726 from maize and Arabidopsis confers pollen resistance to heat and UV radiation by influencing lignin content of sporopollenin显示文摘Sporopollenin in the pollen cell wall protects male gametophytes from stresses.Phenylpropanoid derivatives,including guaiacyl(G)lignin units,are known to be structural components of sporopollenin,but the exact composition of sporopollenin remains to be fully resolved.We analyzed the phenylpropanoid derivatives in sporopollenin from maize and Arabidopsis by thioacidolysis coupled with nuclear magnetic resonance(NMR)and gas chromatography–mass spectrometry(GC–MS).The NMR and GC–MS results confirmed the presence of p-hydroxyphenyl(H),G,and syringyl(S)lignin units in sporopollenin from maize and Arabidopsis.Strikingly,H units account for the majority of lignin monomers in sporopollenin from these species.We next performed a genome-wide association study to explore the genetic basis of maize sporopollenin composition and identified a vesicle-associated membrane protein(ZmVAMP726)that is strongly associated with lignin monomer composition of maize sporopollenin.Genetic manipulation of VAMP726 affected not only lignin monomer composition in sporopollenin but also pollen resistance to heat and UV radiation in maize and Arabidopsis,indicating that VAMP726 is functionally conserved in monocot and dicot plants.Our work provides new insight into the lignin monomers that serve as structural components of sporopollenin and characterizes VAMP726,which affects sporopollenin composition and stress resistance in pollen. | Wenqi Yang Dongdong Yao Haiyang Duan Junli Zhang Yaling Cai Chen Lan Bing Zhao Yong Mei Yan Zheng Erbing Yang Xiaoduo Lu Xuehai Zhang Jihua Tang Ke Yu Xuebin Zhang | 2023 | Plant Communications2023，4，6： | 0 |
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