|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Correction of a genetic disease by CRISPR-Cas9-mediated gene editing in mouse spermatogonial stem cells显示文摘Spermatogonial 干细胞(SSC ) 能在移植以后生产众多的男配偶子进接受者睾丸,介绍为基因治疗和修改基因的动物的连续生产的一条珍贵途径。然而, SSC 的成功的基因操作被限制了,部分由于复杂性和当前可得到的基因编辑技术的低效率。这里,我们证明有效基因修正能用 CRISPR-Cas9 系统被介绍进 SSC。我们使用了 CRISPR-Cas9 系统变异在 SCC 的 EGFP transgene 或内长的 Crygc 基因。变异的 SSC 在移植以后经历了精子发生进不肥沃的老鼠睾丸的生精的小管。圆 spermatids 被产生并且在进成熟卵母细胞的注射以后,支持了显示相应变异的显型的异质接合的后代的生产。而且,在 Crygc 的一个引起疾病的变化(在 SSC 先存在的 Crygc −/−) 能乐意地到加入的 CRISPR-Cas9-induced nonhomologous 结束(NHEJ ) 或指导相同的修理(HDR ) 被修理,导致没有是的离开目标修正的证据带改正的基因的 SSC 线由整个染色体的定序出现。用从这些线产生的圆 spermatids 的授精在 100% 的效率与改正的显型产生了后代。我们的结果表明有效基因由 CRISPR-Cas9 系统在老鼠 SSC 编辑,并且提供在 SSC 经由基因修正治好基因疾病的原则的证明。 | Yuxuan Wu Hai Zhou Xiaoying Fan Ying Zhang Man Zhang Yinghua Wang Zhenfei Xie Meizhu Bai Qi Yin Dan Liang Wei Tang Jiaoyang Liao Chikai Zhou Wujuan Liu Ping Zhu Hongshan Guo Hong Pan Chunlian Wu Huijuan Shi Ligang Wu Fuchou Tang Jinsong Li | 2015 | Cell Research2015，25，1： | 37 |
| 2 | Effects of salidroside on glioma formation and growth inhibition together with improvement of tumor microenvironment显示文摘Objective:To test the effects of salidroside on formation and growth of glioma together with tumor microenvironment.Methods:Salidroside extracted from Rhodiola rosea was purified and treated on human glioma cells U251 at the concentration of 20μg/mL.3-(4,5-dimethylthiazol-2-yl)-2,5-dephenyltetrazolium bromide(MTT)assay for cytotoxicity and flow cytometry(FCM)for cell cycle analysis were performed.Then for in vivo study,xenotransplantation tumor model in nude mice was generated and treated with salidroside at the concentration of 50 mg/kg.d for totally 20 d.Body weight and tumor size were detected every 2 d after the treatment.The levels of 8-isoprostane,superoxide dismutase(SOD)and malondialdehyde(MDA),special markers for oxidative stress,were detected while immunofluoresence staining was performed for astrocyte detection.Results:For in vitro study,salidroside could decrease the viability of human glioma cells U251 and the growth of U251 cells at G0/G1checkpoint during the cell cycle.For in vivo study,salidroside could also inhibit the growth of human glioma tissue in nude mice.The body weight of these nude mice treated with salidroside did not decrease as quickly as control group.In the tumor xenotransplantation nude mice model,mice were found of inhibition of oxidative stress by detection of biomarkers.Furthermore,overgrowth of astrocytes due to the stimulation of oxidative stress in the cortex of brain was inhibited after the treatment of salidroside.Conclusions:Salidroside could inhibit the formation and growth of glioma both in vivo and in vitro and improve the tumor microenvironment via inhibition of oxidative stress and astrocytes. | Yanshan Zhang Yunyi Yao Huijuan Wang Yanqin Guo Hui Zhang Liang Chen | 2013 | Chinese Journal of Cancer Research2013，25，5： | 14 |
| 3 | Microstructure,texture evolution and mechanical properties of cold rolled Ti-32.5Nb-6.8Zr-2.7Sn biomedical beta titanium alloy显示文摘Ti-32.5 Nb-6.8 Zr-2.7 Sn(TNZS,wt%) alloy was produced by using vacuum arc melting method,followed by solution treatment and cold rolling with the area reductions of 50% and 90%.The effects of cold rolling on the microstructure,texture evolution and mechanical properties of the experimental alloy were investigated by optical microscopy,X-ray diffraction,transmission electron microscopy and universal material testing machine.The results showed that the grains of the alloy were elongated along rolling direction and stress-induced α'' martensite was not detected in the deformed samples.The plastic deformation mechanisms of the alloy were related to {112} 111 type deformation twinning and dislocation slipping.Meanwhile,the transition from γ-fiber texture to α-fiber texture took place during cold rolling and a dominant {001} 110_(α-fiber) texture was obtained after 90% cold deformation.With the increase of cold deformation degree,the strength increased owing to the increase of microstrain,dislocation density and grain refinement,and the elastic modulus decreased owing to the increase of dislocation density as well as an enhanced intensity of {001} 110_(α-fiber)texture and a weakened intensity of {111} 112_(γ-fiber)texture.The 90% cold rolled alloy exhibited a great potential to become a new candidate for biomedical applications,since it possesses low elastic modulus(47.1 GPa),moderate strength(883 MPa) and high elastic admissible strain(1.87%),which are superior than those of Ti-6 Al-4 V alloy. | Chunbo Lan Yu Wu Lili Guo Huijuan Chen Feng Chen | 2018 | Journal of Materials Science & Technology2018，34，5： | 10 |
| 4 | Characterization and fine mapping of an early senescence mutant (es-t) in Oryza sativa L.显示文摘An es-t (early senescence-temporary) mutant, produced by ethylene methylsulfonate treatment of strain Nipponbare, was identified in rice. The leaves of es-t appeared yellow at the seedling stage, and had decreased chlorophyll content. Rust spots were found during growth in es-t, especially at the leaf margin and tip. The plants showed a typical early-senescence phenotype at the milky stage. The leaf surface of es-t appeared smoother than wild-type leaves under a scanning electron microscope, because the leaves lack siliceous protuberances around the stoma. Chloroplasts grow abnormally and are filled with many starch grains in es-t. Paraffin section analysis showed that the development of the sclerenchyma cells and vascular bundles were also abnormal in es-t. Genetic analysis indicated that es-t was controlled by a recessive gene, which was finely mapped to a 42-kb interval on chromosome 5. These results will facilitate the positional cloning and functional studies of the gene. | YANG YaoLong RAO YuChun LIU HuiJuan FANG YunXia DONG GuoJun HUANG LiChao LENG YuJia GUO LonBiao ZHANG GuangHeng HU Jiang GAO ZhenYu QIAN Qian ZENG DaLi | 2011 | Chinese Science Bulletin2011，56，23： | 8 |
| 5 | Expression of non-structural protein NS3 gene of Bombyx mori densovirus (China isolate)显示文摘The invertebrate parvovirus Bombyx mori Densonucleosis Virus type 3 (China isolate), named BmDNV-3, is a kind of bidensovirus. It is a new type of virus with unique replication mechanisms. To investigate the effects of the NS3 gene during viral DNA replication, a pair of primers was designed for amplifying NS3 gene of Bombyx mori densovirus (China isolate). Gene NS3 amplified was cloned into a prokaryotic expression vector pET-30a and the donor plasmid pFastBacHTe, respectively. The NS3 protein was expressed in Escherichia coli BL21. The pFastBacHTe-NS3 was transformed to E. coli DH10Bac. The recombinant bacmid baculoviruses (rBacmid-EGFP-NS3) isolated from the white colonies were transfected into BmN-4 cells using a transfection reagent. BmN-4 cells were infected with recom-binant virus to express fusion proteins. The expression of fusion protein around 30 kDa in E. coli BL21 was identified by SDS-PAGE, Western blotting, and mass spectrometry. The expressed NS3 protein by B. mori nucleopolyhedrovirus bacmid system was confirmed by Western blotting using an anti-NS3 polyclonal antibody. And about 45 kDa protein was found. The expressed fusion protein was smaller than the expected size of EGFP-NS3, 55 kDa. Western blotting analysis indicated that EGFP-NS3 protein was expressed in infected lar-vae with smaller molecular size. | Huijuan Yin Qin Yao Zhongjian Guo Fang Bao Wei Yu Jun Li Keping Chen | 2008 | Journal of Genetics and Genomics2008，35，4： | 7 |
| 6 | Prognosis and weaning of elderly multiple organ dysfunction syndrome patients with invasive mechanical ventilation显示文摘 | Xiao Kun Su Longxiang Han Bingchao Guo Chao Feng Lin Jiang Zhaoxu Wang Huijuan Lin Yong Jia Yanhong She Danyang Xie Lixin | 2014 | Chinese Medical Journal2014，，1： | 7 |
| 7 | Would integrated Western and traditional Chinese medicine have more benefits for stroke rehabilitation? A systematic review and meta-analysis显示文摘Background Stroke is a major cause of death or long-term disability worldwide.Many patients with stroke receive integrative therapy consisting of Western medicine(WM)and routine rehabilitation in conjunction with Chinese medicine(CM),such as acupuncture and Chinese herbal medicine.However,there is no available evidence on the effectiveness of the combined use of WM and CM interventions in stroke rehabilitation.Aims The purpose of this meta-analysis is to evaluate the results of all individual studies to assess the combined use of CM and WM in stroke rehabilitation compared with WM only.Methods The Preferred Reporting Items for Systematic Reviews and Meta-Analysis guidelines were followed.MEDLINE,EMBASE,Cochrane and China National Knowledge Infrastructure(CNKI)were searched.The included outcomes were dependency,motor function,depression and swallowing function.Subgroup analysis was performed,and publication bias was assessed using funnel plots.Summary of review 58 studies and 6339 patients were included in the meta-analysis.Subgroup analysis revealed that combined therapy comprising both acupuncture and WM had a superior effect on improving dependency and swallowing function compared with standard WM therapy alone.Potential superiority of combined therapy comprising CM and WM in improving depression compared with standard WM therapy was also found.Conclusions Our results indicate that the combined use of CM and WM could be more efficacious in stroke rehabilitation compared with the use of WM therapy alone.However,most studies were short in duration(2 to 4 weeks)and prone to different types of biases,which prevents making any conclusion regarding the long-term effects and raises concerns regarding true efficacy in context of high likelihood of Hawthorn bias.So,more randomised controlled trials with more rigorous design and longer duration of treatment and follow-up need to be conducted to compare WM alone versus WM and CM combined. | Linda LD Zhong Ya Zheng Alexander Y Lau Norman Wong Liang Yao Xingyao Wu Tengteng Shao Zhenxing Lu Huijuan Li Chun Sum Yuen Jianwen Guo Suzanne Lo Janita Chau Kam Wa Chan Bacon Fung Leung Ng Zhaoxiang Bian Edwin Chau-leung Yu | 2022 | Stroke & Vascular Neurology2022，7，1： | 7 |
| 8 | A sequence of 28S rRNA-derived small RNAs is enriched in mature sperm and various somatic tissues and possibly associates with inflammation显示文摘 | Chu, Chen Yu, Lu Wu, Bin Ma, Li Gou, Lan-Tao He, Miao Guo, Yunli Li, Zhi-Tong Gao, Wei Shi, Huijuan Liu, Mo-Fang Wang, Hongyan Chen, Charlie Degui Drevet, Joel R. Zhou, Yuchuan Zhang, Yonglian | 2017 | Journal of Molecular Cell Biology2017，9，3： | 6 |
| 9 | Preferential binding between intracellular organic matters and Al_(13) polymer to enhance coagulation performance显示文摘Coagulation is the best available method for removing intracellular organic matter(IOM),which is released from algae cells and is an important precursor to disinfection by-products in drinking water treatment. To gain insight into the best strategy to optimize IOM removal, the coagulation performance of two Al salts, i.e., aluminum chloride(AlCl_3) and polyaluminum chloride(PACl, containing 81.2% Al_(13)), was investigated to illuminate the effect of Al species distribution on IOM removal. PACl showed better removal efficiency than AlCl_3 with regard to the removal of turbidity and dissolved organic carbon(DOC), owing to the higher charge neutralization effect and greater stability of pre-formed Al_(13) species. High pressure size exclusion chromatography analysis indicated that the superiority of PACl in DOC removal could be ascribed to the higher binding affinity between Al_(13) polymer and the low and medium molecular weight(MW) fractions of IOM. The results of differential log-transformed absorbance at 254 and 350 nm indicated more significant formation of complexes between AlCl_3 and IOM, which benefits the removal of tryptophan-like proteins thereafter. Additionally,PACl showed more significant superiority compared to AlCl_3 in the removal of <5 kD a and hydrophilic fractions, which are widely viewed as the most difficult to remove by coagulation.This study provides insight into the interactions between Al species and IOM, and advances the optimization of coagulation for the removal of IOM in eutrophic water. | Ruiping Liu Tingting Guo Min Ma Mingquan Yan Jing Qi Chengzhi Hu Gang Liu Huijuan Liu Jiuhui Qu Walter van der Meer | 2019 | Journal of Environmental Sciences2019，31，2： | 5 |
| 10 | Epidemiological Characteristics of Human Anthrax-China,2018-2021显示文摘Introduction:The epidemic of human anthrax is at a low level in China in recent years,but the reported incidence increased in 2021.In order to understand the current landscape of research and knowledge about anthrax in China,the epidemiological characteristics of anthrax in humans from 2018 to 2021 were analyzed and the prevention and control suggestions were proposed.Methods:Surveillance data of anthrax in humans and livestock,together with human outbreaks data during 2018-2021,were collected and analyzed by descriptive statistics methods.The number and proportion of outbreaks,cases and deaths by provincial-level administrative divisions(PLADs),clinical types,and contributing factors were calculated.Results:A total of 1,244 cases of human anthrax and 53 outbreaks were reported from 2018 to 2021 in China.While the incidence of anthrax declined from 2018 to 2020,it increased in 2021.The regions of anthrax were mainly located in the west and the northeast PLADs of China,though cases were reported in some central and eastern PLADs in 2021.Young and middle-aged men involved in animal husbandry were found to be at a higher risk of anthrax.All the reported outbreaks were associated with the exposure of infected livestock.A total of 296 livestock anthrax cases were reported.Conclusions:The increased incidence and wider geographical distribution of human anthrax in 2021 were found to be the result of inadequate supervision of diseased animals as well as updated diagnostic criteria.As such,the monitoring of risk factors and emergency preparation procedures should be strengthened at the national level.In addition,it is also critical to strengthen health education for high-risk occupational groups and strengthen professional training for local clinicians.Finally,more measures should be carried out to strengthen anthrax surveillance in livestock husbandry. | Huijuan Zhang Enmin Zhang Mu Guo Jinrong He Wei Li Jianchun Wei | 2022 | China CDC weekly2022，4，35： | 4 |
| 11 | Designed synthesis of ordered mesoporous graphene spheres from colloidal nanocrystals and their application as a platform for high-performance lithium-ion battery composite electrodes显示文摘三维(3D ) graphene 最近为电气化学的精力存储应用吸引了庞大的注意。然而,当前的方法受不了无能同时控制并且设计 graphene 框架的孔和形态学。这里,我们由自我装配的 Fe 3 O 4 nanocrystal 超点阵。结果的 OMGS 有一个 ultrathin 框架包括很少分层的 graphene,与高度订并且相连的 mesoporosity 和一个高表面区域。这些有益结构并且在有 graphitic 框架的优秀电的传导性的联合,组织上,特征显示为创造好的混合材料的一个理想、一般的平台在锂离子电池(解放) 用作合成电极适合的 OMGS。作为 proof-of-concept 示范, SnO 2 和 GeO 2 nanoparticles 被合并到 OMGS 分别地负担得起 SnO 2@OMGSs 和 GeO 2@OMGSs, ,哪个展览突出的锂存储性质当用作解放阳极时。 | Huijuan Yu Guannan Guo Li Ji Hanwen Li Dong Yang Jianhua Hu Angang Dong | 2016 | Nano Research2016，9，12： | 4 |
| 12 | Laboratory-based Surveillance and Clinical Profile of Sporadic HEV Infection in Shanghai,China显示文摘The study aimed to describe the epidemiological,virological and clinical features of sporadic HEV infection in eastern China.A total of 6112 patient sera were tested for anti-HEV IgG or anti-HEV IgM during one consecutive year(between August 2018 and July 2019).HEV RNA presence was evaluated by RT-PCR and HEV sequences were phylogenetically analyzed.Clinical features of confirmed HEV-infected patients were delineated.The sero-positivity rate of anti-HEV IgG maintained stable around 40%,while an obvious winter spike of anti-HEV IgM prevalence was observed.A total of 111 patients were confirmed of HEV viremia by molecular diagnosis.Subtype 4d was predominant.Phylogenetic analyses suggest that certain strains circulate across species and around the country.Subjects with confirmed current HEV infection had a high median age(58 years)and males were predominant(62.2%).Most patients presented with jaundice(75.7%)and anorexia(68.0%).Significantly elevated levels of liver enzymes and bilirubin were observed.Remarkably,the baseline bilirubin level was positively correlated with illness severity.Pre-existing HBV carriage may deteriorate illness.The clinical burden caused by locally acquired HEV infection is increasing.Surveillance should be enforced especially during the transition period from winter to spring.Patients with higher level of bilirubin at disease onset had slower recovery from HEV infection. | Jie Lu Qing Li Jiayuan Jiang Ziqiang Li Peiyun Wang Zike Sheng Rongtao Lai Huijuan Zhou Wei Cai Hui Wang Qing Guo Honglian Gui Qing Xie | 2021 | Virologica Sinica2021，36，4： | 4 |
| 13 | Equivalent efficacy study of QL1101 and bevacizumab on untreated advanced non-squamous non-small cell lung cancer patients: a phase 3 randomized, double-blind clinical trial显示文摘Objective:This phase 3 study aimed to test equivalence in efficacy and safety for QL1101,a bevacizumab analogue in Chinese patients with untreated locally advanced non-squamous non-small cell lung cancer(NSCLC).Methods:Eligible patients were randomly assigned 1:1 to receive carboplatin and paclitaxel in combination with either QL1101 or bevacizumab,15 mg/kg every 3-week for 6 cycles.This was followed by maintenance treatment with single agent QL1101 every 3-week.The primary end-point was objective response rate(ORR),with secondary end-points being progression-free survival(PFS),overall survival(OS),disease control rate(DCR),and adverse events(AEs).Results:Of 675 patients,535 eligible patients were randomized to the QL1101 group(n=269)and bevacizumab group(n=266).ORRs were 52.8%and 56.8%,respectively,for the QL1101 and bevacizumab groups,with an ORR hazard ratio 0.93(95%confidence interval:0.8-0131.1).The PFS,OS,DCR,and AEs were comparable between the 2 groups,which remained the same after stratification according to epidermal growth factor receptor mutation or smoking history.Conclusions:QL1101 showed similar efficacy and safety profiles as compared to bevacizumab among Chinese patients with untreated locally advanced non-squamous NSCLC. | Tianqing Chu Jun Lu Minghong Bi Helong Zhang Wu Zhuang Yan Yu Jianhua Shi Zhendong Chen Xiaochun Zhang Qisen Guo Quan Liu Huijuan Wu Jian Fang Yi Hu Xiuwen Wang Cuicui Han Kai Li Baohui Han | 2021 | Cancer Biology & Medicine2021，18，3： | 4 |
| 14 | Implantable CMOS neuro-stimulus chip for visual prosthesis显示文摘A prototype chip with 2×2 pixels for implanting in blind patients affected by outer retinal degeneration is presented in this paper.This visual prosthesis chip imitates the degenerated photoreceptor cells,senses the incident light and stimulates the remaining healthy layers of retina or optic nerve.Each pixel integrates photodiode and stimulus pulse generator,converting the illumination on the eyes into 3-bit resolution bi-phasic current pulses.On-chip charge cancellation modules are used to discharge each electrode site for tissue safety.The prototype chip is designed and fabricated in 0.18-μm N-well CMOS (complementary metal oxide semiconductor) 1P6M Mix-signal process,with a ±2.5 V dual voltage supply.The functionality of the fabricated chip is demonstrated on anesthetized rabbits.Neural responses in visual cortex are successfully evoked by the neuro-stimulus chip through an on-board trigger interface and flexible electrode. | ZHANG Xu PEI WeiHua HUANG BeiJu WANG ShuJing GUAN Ning GUO Kai WANG Yu GUI Qiang CHEN Jin WANG Kai WU HuiJuan LI XiaoXin LI Kai CHEN HongDa | 2011 | Science China(Information Sciences)2011，54，4： | 3 |
| 15 | Interaction Between Variations in Dopamine D2 and Serotonin 2A Receptor is Associated with Short-Term Response to Antipsychotics in Schizophrenia显示文摘Dear Editor,Schizophrenia is a chronic and debilitating brain disorder,which has a strong genetic component with heritability ranging from 66%to 85%[1,2].Currently,antipsychotic drugs remain the most effective treatment for the psychotic symptoms of schizophrenia[3].Because of the severe sideeffects of first-generation antipsychotics(FGAs),secondgeneration antipsychotics(SGAs)have become more widely used in the treatment of schizophrenia. | Liansheng Zhao Huijuan Wang Yamin Zhang Jinxue Wei Peiyan Ni Hongyan Ren Gang Li Qiang Wang Gavin P Reynolds Weihua Yue Wei Deng Hao Yan Liwen Tan Qi Chen Guigang Yang Tianlan Lu Lifang Wang Fuquan Zhang Jianli Yang Keqing Li Luxian Lv Qingrong Tan Yinfei Li Hua Yu Hongyan Zhang Xin Ma Fude Yang Lingjiang Li Chuanyue Wang Huiyao Wang Xiaojing Li Wanjun Guo Xun Hu Yang Tian Xiaohong Ma Jeremy Coid Dai Zhang Chao Chen Tao Li Chinese Antipsychotics Pharmacogenomics Consortium | 2019 | Neuroscience Bulletin2019，35，6： | 3 |
| 16 | Neck dissection for recurrent and persistent lymph nodes of nasopharyngeal carcinoma after radiotherapy: effect and choice显示文摘目的将在放射疗法以后与周期性、坚持的淋巴节点为鼻咽的癌的病人调查最好的外科的模式。有在放射疗法以后的周期性、坚持的淋巴节点的鼻咽的癌的 88 个病人的临床的数据回顾地被分析的方法。在层次之中的深奥淋巴节点和关系的层次被分析;幸存率和包括激进的颈解剖(RND ) 的外科的模式的周期性的率,修改的激进的颈解剖(MRND ) ,选择的颈解剖(SND ) ,和淋巴节点切除术(LNR ) 被分析;手术后的放射疗法的角色被评估。周期性、坚持的淋巴节点主要在水平 II 定位了的结果( 1 )(55.6%和58.6%,分别地),下次是水平 III 并且很少在水平 IV , V ,并且我,而是层次 IV 的数字, V ,并且我与忍受癌症的淋巴结是相对多于临床的测量的。(2 ) 通常,有涉及水平 III 和 IV 的淋巴节点的病人把另外的层次同时深奥;百分比分别地是 63.6% 和 88.9% 。然而,在水平 II 和 V 的淋巴节点主要被孤立。(3 )5 年的幸存率和整个组的周期性的率分别地是 42.77% 和 22.7% 。(4 ) RND, MRND, SND,和 LMR 组的 5 年的幸存率分别地是 39.75% , 60.00% , 37.87% ,和 44.10% ;差别是不足道的(木头等级 = 1.0, P = 0.8011 ) ;在广泛、本地的外科组之间的周期性的率是不足道的(X 2= 0.470, P = 0.493 ) 。(5 ) 有或没有手术后的放射疗法的病人的 5 年的幸存率分别地是 39.06% 和 45.26% ;差别是不足道的(木头等级 = 0.06, P = 0.8138 ) 。当周期性、坚持的淋巴结是超过一水平时,广泛的外科被推荐的结论包含了或很大或不可移动不那样, SND 应该被执行,手术后的放射疗法如果必要是重要赔偿。 | Liangping Xia Zongyuan Zeng Zhuming Guo Guifang Guo Bei Zhang Huijuan Qiu Feifei Zhou | 2009 | The Chinese-German Journal of Clinical Oncology2009，8，2： | 3 |
| 17 | Initial neutrophil lymphocyte ratio is superior to platelet lymphocyte ratio as an adverse prognostic and predictive factor in metastatic colorectal cancer显示文摘 | Wenzhuo He Chenxi Yin Guifang Guo Chang Jiang Fang Wang Huijuan Qiu Xuxian Chen Ruming Rong Bei Zhang Liangping Xia | 2013 | Medical Oncology2013，，1： | 2 |
| 18 | Positional cloning of PmCH1357 reveals the origin and allelic variation of the Pm2 gene for powdery mildew resistance in wheat显示文摘Powdery mildew, caused by the biotrophic fungus Blumeria graminis f. sp. tritici(Bgt), is a prevalent disease in common wheat(Triticum aestivum L.) and causes serious yield losses worldwide. We used a map-based approach to clone the major broad-spectrum powdery mildew resistance gene Pm CH1357 from wheat breeding line CH1357. Pm CH1357 was mapped to a 526 kb region containing only Traes CS5 D01 G044600. The Traes CS5 D01 G044600 sequence of the susceptibility allele in Taichung 29(TC29) was identical to that in Chinese Spring, whereas the sequence of the resistance allele in CH1357 was identical to Pm2a previously cloned from the germplasm Ulka/*8Cc. The susceptibility allele in TC29 contained a 7 bp deletion in exon 1, resulting in loss of 856 of the 1277 amino acids in the predicted nucleotide-binding domain leucine-rich repeat containing Pm2a protein.Pm CH1357/Pm2a sequence was also isolated from the Chinese wheat landraces and cultivars that were previously reported to possess the resistance gene Pm2b, Pm2c,PmLX66, or PmND399. The Pm CH1357/Pm2a resistance allele was present in 10 of 495 accessions in core germplasm and contemporary cultivars from China and the USA. A newly developed diagnostic marker for the 7 bp In Del in the resistance gene can be used to eliminate the susceptibility allele in wheat breeding programs. | Fang Chen Haiyan Jia Xiaojun Zhang Linyi Qiao Xin Li Jun Zheng Huijuan Guo Carol Powers Liuling Yan Zhijian Chang | 2019 | The Crop Journal2019，7，6： | 2 |
| 19 | Friction and wear behavior of the hybrid PTFE/cotton fabric composites filled with TiO2 nanoparticles and modified TiO2 nanoparticles显示文摘 | ZHANG Huijuan ZHANG Zhaozhu GUO Fang | 2009 | Polymer Engineering & Science2009，49，1： | 1 |
| 20 | Chemical characteristic and anticoagulant activity of the sulfated polysaccharide isolated from Monostroma latissimum (Chlorophyta)显示文摘 | Wenjun Mao Hongyan Li Yi Li Huijuan Zhang Xiaohui Qi Haihong Sun Yin Chen Shoudong Guo | 2008 | International Journal of Biological Macromolecules2008，，1： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员