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| 1 | Adventitious root formation in rice requires OsGNOM1 and is mediated by the OsPINs family显示文摘在谷物的含纤维的根系统包括首先偶然根(艺术) ,它在营养素和水举起起重要作用。关于位于 AR 开发下面的分子的机制的当前的知识仍然是有限的。我们这里报导四米饭(Oryza sativa L.) 的隔离异种从不同基因背景,所有哪个在 AR 是有缺点的形成。这些异种展出了侧面的根(LR ) 和 gravitropism 的部分损失的减少的数字。也显示的异种提高了敏感到 N-1-naphthylphthalamic 酸,极的植物生长素运输的一个禁止者(轻拍) ,显示变化影响了植物生长素运输。用四异种之一的位置的克隆表明它被一个 guanine 核苷酸交换因素的 loss-of-function 为 ADP-ribosylation 因素(OsGNOM1 ) 引起。转基因的植物显示出的 promoter::GUS 的 RT-PCR 和分析那 OsGNOM1 在 AR primordia,脉管的纸巾, LR,根尖端,叶子,花药和词根静脉被表示,与类似于植物生长素的一个分发模式。另外, OsPIN2, OsPIN5b 和 OsPIN9 的表情在异种被改变。一起拿,这些调查结果显示 OsGNOM1 通过调整影响艺术的形成轻拍。 | Shiping Liu Jirong Wang Lu Wang Xiaofei Wang Yanhong Xue Ping Wu Huixia Shou | 2009 | Cell Research2009，19，9： | 29 |
| 2 | Overexpression of OsPAP10a, A Root-Associated Acid Phosphatase, Increased Extracellular Organic Phosphorus Utilization in Rice显示文摘Phosphorus (P) deficiency is a major limitation for plant growth and development. Among the wide set of responses to cope with low soil P, plants increase their level of intracellular and secreted acid phosphatases (APases), which helps to catalyze inorganic phosphate (Pi) hydrolysis from organophosphates. In this study we characterized the rice (Oryza sativa) purple acid phosphatase 10a (OsPAP10a). OsPAP10a belongs to group Ia of purple acid phosphatases (PAPs), and clusters with the principal secreted PAPs in a variety of plant species including Arabidopsis. The transcript abundance of OsPAP10a is specifically induced by Pi deficiency and is controlled by OsPHR2, the central transcription factor controlling Pi homeostasis. In gel activity assays of root and shoot protein extracts, it was revealed that OsPAP10a is a major acid phosphatase isoform induced by Pi starvation. Constitutive overexpression of OsPAP10a results in a significant increase of phosphatase activity in both shoot and root protein extracts. In vivo root 5-bromo-4-chloro-3-indolyl-phosphate (BCIP) assays and activity measurements on external media showed that OsPAP10a is a root-associated APase. Furthermore, overexpression of OsPAP10a significantly improved ATP hydrolysis and utilization compared with wild type plants. These results indicate that OsPAP10a can potentially be used for crop breeding to improve the efficiency of P use. | Jingluan Tian Chuang Wang Qian Zhang Xiaowei He James Whelan Huixia Shou | 2012 | Journal of Integrative Plant Biology2012，54，9： | 18 |
| 3 | Simultaneous changes in seed size, oil content and protein content driven by selection of SM/EEIhomologues during soybean domestication显示文摘Soybean accounts for more than half of the global production of oilseed and more than a quarter of the protein used globally for human food and animal feed.Soybean domestication involved parallel increases in seed size and oil content,and a concomitant decrease in protein content.However,science has not yet discovered whether these effects were due to selective pressure on a single gene or multiple genes.Here,re-sequencing data from>800 genotypes revealed a strong selection during soybean domestication on GmSWEETlOa.The selection of GmSWEETlOa conferred simultaneous increases in soybean-seed size and oil content as well as a reduction in the protein content.The result was validated using both near-isogenic lines carrying substitution of haplotype chromosomal segments and transgenic soybeans.Moreover,GmSWEETlOb was found to be functionally redundant with its homologue GmSWEETlOa and to be undergoing selection in current breeding,leading the the elite allele GrnSWEETlOb,a potential target for present-day soybean breeding.Both GmSWEETlOa and GmSWEETlOb were shown to transport sucrose and hexose,contributing to sugar allocation from seed coat to embryo,which consequently determines oil and protein contents and seed size in soybean.We conclude that past selection of optimal GmSWEETlOa alleles drove the initial domestication of multiple soybean-seed traits and that targeted selection of the elite allele GmSWEETlOb may further improve the yield and seed quality of modern soybean cultivars. | Shoudong Wang Shulin Liu Jie Wang Kengo Yokosho Bin Zhou Ya-Chi Yu Zhi Liu Wolf BFrommer Jian Feng Ma Li-Qing Chen Yuefeng Guan Huixia Shou Zhixi Tian | 2020 | National Science Review2020，7，11： | 17 |
| 4 | Two soybean bHLH factors regulate response to iron deficiency显示文摘Iron is an indispensable micronutrient for plant growth and development. Limited bioavailability of Fe in the soil leads to iron deficiency chlorosis in plants and yield loss. In this study, two soybean basic helix-loophelix transcription factors, Gmb HLH57 and Gmb HLH300,were identified in response to Fe-deficiency. Both transcription factors are expressed in roots and nodules,and are induced by Fe deficiency; these patterns were confirmed in transgenic hairy roots expressing constructs of the endogenous promoters fused to a GUS reporter gene. Bimolecular fluorescence complementation, yeast two-hybrid and coimmunoprecipitation(co-IP) assays indicated a physical interaction between Gmb HLH57 and Gmb HLH300. Studies on transgenic soybeans Reseoverexpressing Gmb HLH57 and Gmb HLH300 revealed that overexpression of each transcription factor, alone,results in no change of the responses to Fe deficiency,whereas overexpression of both transcription factors upregulated the downstream Fe uptake genes and increased the Fe content in these transgenic plants.Compared to wild type, these double overexpression transgenic plants were more tolerant to Fe deficiency.Taken together, our findings establish that Gmb HLH57 and Gmb HLH300 are important transcription factors involved in Fe homeostasis in soybean. | Lin Li Wenwen Gao Qi Peng Bin Zhou Qihui Kong Yinghui Ying Huixia Shou | 2018 | Journal of Integrative Plant Biology2018，60，7： | 10 |
| 5 | Assessment of transgenic maize events produced by particle bombardment or Agrobacterium -mediated transformation显示文摘 | Huixia Shou Bronwyn R. Frame Steven A. Whitham Kan Wang | 2004 | Molecular Breeding2004，，2： | 2 |
| 6 | Irreproducibility of the soybean pollen-tube pathway transformation procedure显示文摘 | Huixia Shou Reid G. Palmer Kan Wang | 2002 | Plant Molecular Biology Reporter2002，，4： | 1 |
| 7 | Functional characterization of the rice SPX‐MFS family reveals a key role of OsSPX‐MFS1 in controlling phosphate homeostasis in leaves显示文摘 | Chuang Wang Wei Huang Yinghui Ying Shuai Li David Secco Steve Tyerman James Whelan Huixia Shou | 2012 | New Phytologist2012，，1： | 1 |
| 8 | Assessment of conditions affecting Agrobacterium-mechated soybean transformation using the cotyledonary node explant 显示文摘 | MARGIE M PAZ HUIXIA SHOU ZIBIAO GUO | 2004 | Euphytica2004，136，： | 1 |
| 9 | Agrobacterium tumefaciens- mediated transformation of maize embryos using a standard binary vector system显示文摘 | Bronwyn R Frame Huixia Shou | 2002 | Plant Physiol2002，129，： | 1 |
| 10 | Expression of the Nicotiana protein kinase (NPK1) enhanced droughe tolerance in transgenic maize显示文摘 | Huixia Shou Patricia Bordallo Kan Wang | 2004 | Journal of Experimental Botany2004，55，399： | 1 |
| 11 | Assessment of transgenic maize events produced by particle bombardment or Agrobacterium-mediated transformation显示文摘 | Huixia Shou Bronwyn R. Frame Steven A. Whitham Kan Wang | 2004 | Molecular Breeding2004，，2： | 1 |
| 12 | Agrobacterium tumefaciens-mediated transformation of maize embryos using a standard binary vector system显示文摘 | Bronwyn R Frame Huixia Shou Rachel K | 2002 | Plant Physiol2002，129，： | 1 |
| 13 | Cytokinin-Mediated Cell Cycling Arrest of Pericycle Founder Cells in Lateral Root Initiation of Arabidopsis显示文摘 | Xiang Li Xiaorong Mo Huixia Shou and Ping Wu | 2006 | Plant Cell Physiol2006，47，8： | 1 |
| 14 | Agrobacterium tumefaciens-mediated transformation of maize embryos using a standard binary vector system显示文摘 | Bronwyn R Frame Huixia Shou Rachel K | 2002 | Plant Physiol2002，129，： | 1 |
| 15 | Expression of an active tobacco mitogen-activated protein kinase kinase kinase enhances freezing tolerance in transgenic maize显示文摘 | Shou Huixia Bordallo P Fan Jianbing | 2004 | Proc Natl Acad Sci U S A2004，101，9： | 1 |
| 16 | Vertical Transmission of Severe Acute Respiratory Syndrome Coronavirus 2:Current Evidence and Perspectives显示文摘With the widespread transmission of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),the medical community has organized efforts to determine a series of clinical questions about the virus.Critical questions have also arisen in the field of maternal-fetal medicine and have received increasing attention,as physiological and immunologic changes in pregnancy might potentially increase the susceptibility to SARS-CoV-2 infection and complications of the virus.1 Many early reports indicated that pregnant women with SARS-CoV-2 infection were not prone to experiencing severe disease. | Chong Shou Chen Wang Huixia Yang | 2023 | Maternal-Fetal Medicine2023，5，2： | 1 |
| 17 | Expression of a synthetic E. coli heat-labile enterotoxin B sub-unit (LT-B) in maize显示文摘 | Rachel Chikwamba Jennifer McMurray Huixia Shou Bronwyn Frame Sue Ellen Pegg Paul Scott Hugh Mason Kan Wang | 2002 | Molecular Breeding2002，，4： | 1 |
| 18 | Cytokinin- mediated cell cycling arrest of pericycle founder cells in lateral root initiation of Arabidopsis 显示文摘 | LI Xiang MO Xiaorong SHOU Huixia | 2006 | Plant Cell Physi- ol2006，47，8： | 1 |
| 19 | Expression of the Nicotiana protein kinase ( NPK1 ) enhanced drought tolerance in transgenic maize 显示文摘 | Huixia Shou Patricia Bordallo Kan Wang | 2004 | Journal of Experimental Botany2004，55，： | 1 |
| 20 | Evaluation and application of an efficient plant DNA extraction protocol for laboratory and field testing显示文摘Nucleic acids in plant tissue lysates can be captured quickly by a cellulose filter paper and prepared for amplification after a quick purification.In this study,a published filter paper strip method was modified by sticking the filter paper on a polyvinyl chloride resin(PVC)sheet.This modified method is named EZ-D,for EASY DNA extraction.Compared with the original cetyl trimethylammonium bromide(CTAB)method,DNA extracted by EZ-D is more efficient in polymerase chain reaction(PCR)amplification due to the more stable performance of the EZ-D stick.The EZ-D method is also faster,easier,and cheaper.PCR analyses showed that DNA extracted from several types of plant tissues by EZ-D was appropriate for specific identification of biological samples.A regular PCR reaction can detect the EZ-D-extracted DNA template at concentration as low as 0.1 ng/μL.Evaluation of the EZ-D showed that DNA extracts could be successfully amplified by PCR reaction for DNA fragments up to 3000 bp in length and up to 80%in GC content.EZ-D was successfully used for DNA extraction from a variety of plant species and plant tissues.Moreover,when EZ-D was combined with the loop-mediated isothermal amplification(LAMP)method,DNA identification of biological samples could be achieved without the need for specialized equipment.As an optimized DNA purification method,EZ-D shows great advantages in application and can be used widely in laboratories where equipment is limited and rapid results are required. | Qi WANG Xiaoxia SHEN Tian QIU Wei WU Lin LI Zhi'an WANG Huixia SHOU | 2021 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2021，22，2： | 1 |
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