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| 1 | Birth of fertile bimaternal offspring following intracytoplasmic injection of parthenogenetic haploid embryonic stem cells显示文摘 | Zhikun Li Haifeng Wan Guihai Feng Leyun Wang Zhengquan He YukaiWang Xiu-Jie Wang Wei Li Qi Zhou Baoyang Hu | 2016 | Cell Research2016，26，1： | 15 |
| 2 | Continuous and comprehensive atmospheric observations in Beijing:a station to understand the complex urban atmospheric environment显示文摘Due to profound impact on climate and human health,air quality has attracted attention from all levels of the civil society.The key step in the provision of required tools for the society to tackle the complex air quality problem is to characterize it in a comprehensive manner with a long-term perspective.Here,we describe a continuous and comprehensive observation station and its accompanying state-ofthe-art instrumentation that was established to investigate the complex urban atmospheric environment in a rapidly developing Chinese Megacity.The station,located in downtown Beijing,aims to study air quality by identifying the major atmospheric pollutants and key processes determining their formation and loss mechanisms.A few hundreds of parameters are continuously measured with the state-of-the-art instruments,including trace gas concentrations,aerosol particle size distributions,and mass concentrations,covering aerosol particle chemical composition from molecules to micrometer-sized aerosol particles.This produced long-term,comprehensive big data with around 1�10^(11)bytes per year.In this paper,we provide an overview on the facilities of the station,the instrumentation used,the workflow of continuous observations and examples of results from 2018 to 2019 and a basis for establishing a modern long-term,comprehensive atmospheric urban observation station in other megacities. | Yongchun Liu Chao Yan Zemin Feng Feixue Zheng Xiaolong Fan Yusheng Zhang Chang Li Ying Zhou Zhuohui Lin Yishou Guo Ying Zhang Li Ma Wenshuo Zhou Zhikun Liu Lubna Dada Kaspar Dallenbach Jenni Kontkanen Runlong Cai Tommy Chan Biwu Chu Wei Du Lei Yao Yonghong Wang Jing Cai Juha Kangasluoma Tom Kokkonen Joni Kujansuu Anton Rusanen Chenjuan Deng Yueyun Fu Rujing Yin Xiaoxiao Li Yiqun Lu Yiliang Liu Chaofan Lian Dongsen Yang Weigang Wang Maofa Ge Yuesi Wang Douglas R.Worsnop Heikki Junninen Hong He Veli-Matti Kerminen Jun Zheng Lin Wang Jingkun Jiang Tuukka Petaja Federico Bianchi Markku Kulmala | 2020 | Big Earth Data2020，4，3： | 3 |
| 3 | A new fragment re-allocation strategy for NoSQL database systems显示文摘 | Zhikun CHEN Shuqiang YANG Shuang TAN Li HE Hong YIN Ge ZHANG | 2015 | Frontiers of Computer Science2015，9，1： | 3 |
| 4 | Photophysical and photosensitive properties of Elsinochrome A显示文摘In this paper, the photophysical and photosensitive properties of Elsinochrome A (EA), another member of perylenequinone photosensitizers derived via artificially biological synthesis, were stud- ied by taking hypocrellin A (HA) and hypocrellin B (HB) as the references. It was found that the quantum yield of singlet oxygen produced via the photosensi- tization of EA was 0.98 with HB (0.84) as a reference. Furthermore, the semiquinone anion could be pro- duced via photosensitization of EA in oxygen-free solution. Besides, taking advantage of nanosecond time-resolved spectral technique, the transient triplet absorption spectra and the lifetime of EA in cyclo- hexane were determined by the use of the ground- state depletion method. | LI Cong HE Yanping OU Lingcheng TIAN Maojun YAO Zhikun GUO Mengbi | 2006 | Chinese Science Bulletin2006，51，9： | 1 |
| 5 | A novel square root cubature Kalman filter using model-enhanced Gaussian process state-space models显示文摘 | He Zhikun Liu Guangbin Zhao Xijing | 2012 | Journal of Theoretical and Applied Information Technology2012，45，2： | 1 |
| 6 | Asymmetric catalytic nitrooxylation and azidation of β-keto amides/esters with hypervalent iodine reagents显示文摘Chiral Lewis acid-catalyzed enantioselective nitrooxylation of cyclic and acyclicβ-keto amides/esters with hypervalent iodine(III)reagents is reported.A number ofα-nitrooxy-β-keto amides/esters were obtained with good yields and high enantioselectivities by using bench-stable nitratobenziodoxole under mild conditions. | Changqiang He Zhikun Wu Yuqiao Zhou Weidi Cao Xiaoming Feng | 2022 | Organic Chemistry Frontiers2022，9，3： | 1 |
| 7 | Generation ofαGal-enhanced bifunctional tumor vaccine显示文摘Hepatocellular carcinoma(HCC)is a common malignant tumor with poor prognosis and high mortality.In this study,we demonstrated a novel vaccine targeting HCC and tumor neovascular endothelial cells by fusing recombinant MHCC97H cells expressing porcine a-1,3-galactose epitopes(a Gal)and endorphin extracellular domains(END)with dendritic cells(DCs)from healthy volunteers.END^(+)/Gal^(+)-MHCC97H/DC fusion cells induced cytotoxic T lymphocytes(CTLs)and secretion of interferon-gamma(IFN-γ).CTLs targeted cells expressing a Gal and END and tumor angiogenesis.The fused cell vaccine can effectively inhibit tumor growth and prolong the survival time of human hepatoma mice,indicating the high clinical potential of this new cell based vaccine. | Jian He Yu Huo Zhikun Zhang Yiqun Luo Xiuli Liu Qiaoying Chen Pan Wu Wei Shi Tao Wu Chao Tang Huixue Wang Lan Li Xiyu Liu Yong Huang Yongxiang Zhao Lu Gan Bing Wang Liping Zhong | 2022 | Acta Pharmaceutica Sinica B2022，12，7： | 0 |
| 8 | Generation of in situ CRISPR-mediated primary and metastatic cancer from monkey liver显示文摘Non-human primates(NHPs)represent the most valuable animals for drug discovery.However,the current main challenge remains that the NHP has not yet been used to develop an efficient translational medicine platform simulating human diseases,such as cancer.This study gen erated an in situ gen e-editi ng approach to in duce efficie nt loss-of-function mutations of Pten and p53 genes for rapid modeling primary and metastatic liver tumors using the CRISPR/Cas9 in the adult cyno molgus mon key.Un der ultraso und guida nee,the CRISPR/Cas9 was injected into the cyno molgus mon key liver through the in trahepatic portal vein.The results showed that the ultraso un d-guided CRISPR/Cas9 resulted in in dels of the Pten and p53 genes in seve n out of eight monkeys.The best mutation efficiencies for Pten and p53 were up to 74.71%and 74.68%,respectively.Furthermore,the morbidity of primary and exte nsively metastatic(lung,splee n,lymph nodes)hepatoma in CRISPR-treated mon keys was 87.5%.The ultraso un d-guided CRISPR system could have great potential to successfully pursue the desired target genes,thereby reducing possible side effects associated with hitting no n-specific off-target genes,and sign ifica ntly in creasing more efficie ncy as well as higher specificity of in situ gene editi ng in vivo,which holds promise as a powerful,yet feasible tool,to edit disease genes to build corresp on ding huma n disease models in adult NHPs and to greatly accelerate the discovery of new drugs and save economic costs. | Liping Zhong Yong Huang Jian He Nuo Yang Banghao Xu Yun Ma Junjie Liu Chao Tang Chengpiao Luo Pan Wu Zongqiang Lai Yu Huo Tao Lu Dongni Huang Wenlin Gong Lu Gan Yiqun Luo Zhikun Zhang Xiyu Liu Yongxiang Zhao | 2022 | Signal Transduction and Targeted Therapy2022，7，1： | 0 |
| 9 | Generation and Simulation of Basic Maneuver Action Library for 6-DOF Aircraft by Reinforcement Learning显示文摘The development of modern air combat requires aircraft to have certain intelligent decision-making ability.In some of the existing solutions,the automatic control of aircraft is mostly composed of the upper mission decision and the lower control system.Although the underlying PID(Proportional Integral Derivative)based controller has a good performance in stable conditions,it lacks stability in complex environments.So,we need to design a new system for the problem of aircraft decision making.Studies have shown that the behavior of an aircraft can be viewed as a combination of several basic maneuvers.The establishment of aircraft basic motion library will effectively reduce the difficulty of upper aircraft control.Given the good performance of reinforcement learning to solve the problem with continuous action space,in this paper,reinforcement learning is used to control the aircraft’s rod and rudder to generate a basic maneuver action library,and the flight of the aircraft under the 6 degrees of freedom(6-DOF)simulation engine is effectively controlled.The simulation results verify the feasibility of the method on a visual simulation platform. | Jinlin Wang Jitao Teng Yang He Hongyu Yang Yulong Ji Zhikun Tang Ningwei Bai | 2022 | Journal on Internet of Things2022，4，2： | 0 |
| 10 | miR-126 mitigates the osteogenic differentiation of human bone marrow-derived mesenchymal stem cells by targeting the ERK1/2 and Bcl-2 pathways显示文摘Human bone marrow mesenchymal stem cells(hBMMSCs)are a promising cell source for bone engineering owing to their high potential to differentiate into osteoblasts.The objective of the present study is to assess microRNA-126(miR-126)and examine its effects on the osteogenic differentiation of hBMMSCs.In this study,we investigate the role of miR-126 in the progression of osteogenic differentiation(OD)as well as the apoptosis and inflammation of hBMMSCs during OD induction.OD is induced in hBMMSCs,and matrix mineralization along with other ODassociated markers are evaluated by Alizarin Red S(AR)staining and quantitative PCR(qPCR).Gain-and loss-offunction studies are performed to demonstrate the role of miR-126 in the OD of hBMMSCs.Flow cytometry and qPCR-based cytokine expression studies are performed to investigate the effect of miR-126 on the apoptosis and inflammation of hBMMSCs.The results indicate that miR-126 expression is downregulated during the OD of hBMMSCs.Gain-and loss-of function assays reveal that miR-126 upregulation inhibits the differentiation of hBMMSCs into osteoblasts,whereas the downregulation of miR-126 promotes hBMMSC differentiation,as assessed by the determination of osteogenic genes and alkaline phosphatase activity.Furthermore,the miR-126 level is positively correlated with the production of inflammatory cytokines and apoptotic cell death.Additionally,our results suggest that miR-126 negatively regulates not only B-cell lymphoma 2(Bcl-2)expression but also the phosphorylation of extracellular signal-regulated protein kinase(ERK)1/2.Moreover,restoring ERK1/2 activity and upregulating Bcl-2 expression counteract the miR-126-mediated suppression of OD in hBMMSCs by promoting inflammation and apoptosis,respectively.Overall,our findings suggest a novel molecular mechanism relevant to the differentiation of hBMMSCs into osteoblasts,which can potentially facilitate bone formation by counteracting miR-126-mediated suppression of ERK1/2 activity and Bcl-2 expression. | Ying Zhang Yiping Dong Qiushi Wei Zhikun Zhuang Youwen Liu Qiang Yuan Wei He Zhenhao Jing Jitian Li Peifeng Li Leilei Zhang Zhinan Hong Ning Zhang Haibin Wang Wuyin Li | 2023 | Acta Biochimica et Biophysica Sinica2023，55，3： | 0 |
| 11 | A recombinant Newcastle disease virus expressing MMP8 promotes oncolytic efficacy显示文摘Oncolytic virus is an emerging anti-cancer strategy. However, extracellular matrix(ECM), as a physical barrier, limits virus spread within the tumor. To overcome the obstacle, we constructed a recombinant Newcastle disease virus(NDV) expressing matrix metalloproteinase(MMP8)(NDV-MMP8) using with reverse genetic technology. In vitro, NDV-MMP8 was identified and verified by WB and ELISA. Cell viability was detected by CCK-8 assay. In vivo, we established two liver cancer xenograft models. NDV-MMP8 was injected into the tumor to observe the tumor volume and survival of mice. The changes in extracellular matrix were observed by Masson’s trichrome staining. Virus expression in tumor tissues was detected by immunofluorescence assay. The virus titer in tumor tissues was detected by TCID50. Histopathological changes were detected by hematoxylin and eosin(HE) and terminal deoxynucleotidyl transferase d UTP nick end labeling(TUNEL) staining. Intratumoral administration of NDV-MMP8 can effectively degrade ECM, promote the spread of the virus within the tumor, and reduce tumor growth rate. Therefore, the method of increasing intratumoral virus accumulation by degradation of the ECM to enhance the oncolytic effect has great potential for clinical application. | Tong Guo Xiuli Liu Zhikun Zhang Yiqun Luo Tong Li Lan Li Huixue Wang Yong Huang Jian He Qiaoying Chen Yongxiang Zhao Lu Gan Liping Zhong | 2021 | Chinese Chemical Letters2021，32，12： | 0 |
| 12 | Atomically Precise Metal Nanoclusters as Single Electron Transferers for Hydroborylation显示文摘The emergence of metal nanoclusters with atomically precise compositions and structures provides an opportunity for in-depth investigation of catalysis mechanisms and structure−property correlations at the nanoscale.However,a serious problem for metal nanocluster catalysts is that the ligands inhibit the catalytic activity through deactivating the surface of the nanoclusters.Here,we introduce a novel catalytic mode for metal nanoclusters,in which the nanoclusters initiate the catalysis via single electron transfer(SET)without destroying the integrity of nanoclusters,providing a solution for the contradiction between activity and stability of metal nanoclusters.We illustrated that the novel activation mode featured low catalyst loading(0.01 mol%),high TOF,mild reaction conditions,and easy recycling of catalyst in alkyne hydroborylation,which often suffered from poor selectivity,low functional group tolerance,etc.Furthermore,the catalyst[Au_(1)Cu_(14)(TBBT)_(12)(PPh_(3))_(6)]^(+)(TBBTH:p-tert-butylthiophenol)can be applied in highly efficient tandem processes such as hydroborylation−deuteration and hydroborylation−isomerization,demonstrating the utility of the introduced activation mode for metal nanoclusters. | Wanli Zhu Sheng Zhang Weigang Fan Ying Yang Hongliang Zhao Wenwen Fei Hong Bi Jian He Man-Bo Li Zhikun Wu | 2023 | Precision Chemistry2023，1，3： | 0 |
| 13 | Combined synthesis of interconvertible Au11Cd and Au26Cd5 for photocatalytic oxidations involving singlet oxygen显示文摘A novel Au11Cd nanocluster was synthesized by developing a combined method and controlling the kinetics, and another Au26Cd5nanocluster was also obtained after the conditions were changed in the same reaction, which could transfer to Au11Cd in a two-way style. Both alloy nanoclusters can photocatalyze the production of singlet oxygen(1O_(2)) and exhibit enhanced efficiencies in photocatalyzing two kinds of organic oxidations involving singlet oxygen compared with their non-alloyed mother nanoclusters, indicating that the Cd-doping might be an efficient way to enhance the photocatalysis performance of gold nanoclusters and metal nanoclusters are promising photocatalysts for organic oxidation involving singlet oxygen. | Jiafeng Zou Wenwen Fei Yao Qiao Ying Yang Zongbing He Lei Feng Man-Bo Li Zhikun Wu | 2023 | Chinese Chemical Letters2023，34，4： | 0 |
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