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| 1 | Highly Efficient A. T to G. C Base Editing by Cas9n- 3uided tRNA Adenosine Deaminase in Rice显示文摘 | Fang Yan Yongjie Kuang Bin Ren Jingwen Wang Dawei Zhang Honghui Lin Bing Yang Xueping Zhou Huanbin Zhou | 2018 | Molecular Plant2018，11，4： | 26 |
| 2 | Cas9-NG Greatly Expands the Targeting Scope of the Genome-Editing Toolkit by Recognizing NG and Other Atypical PAMs in Rice显示文摘CRISPR technologies enabling precise genome manipulation are valuable for gene function studies and molecular crop breeding. However, the requirement of a protospacer adjacent motif (PAM)y such as NGG and TTN, for Cas protein recognition restricts the selection of targetable genomic loci in practical applications of CRISPR technologies. Recently Cas9-NG, which recognizes a minimal NG PAM, was reported to expand the targeting space of genome editing in human cells, but it remains unclear whether this Cas9 variant can be used in plants. In this study, we evaluated the nuclease activity of Cas9-NG toward various NGN PAMs by targeting endogenous genes in transgenic rice. We found that Cas9-NG edits all NGG, NGA, NGT, and NGC sites with impaired activity, while the gene-edited plants were dominated by monoallelic mutations. Cas9-NG-engineered base editors were then developed and used to generate O s B Z R I gainof- function plants that can not be created by other available Cas9-engineered base editors. Moreover, we showed that a Cas9-NG-based transcriptional activator efficiently upregulated the expression of endogenous target genes in rice. In addition, we discovered that Cas9-NG recognizes NAC, NTG, NTT, and NCG apart from NG PAM. Together, these findings demonstrate that Cas9-NG can greatly expand the targeting scope of genome-editing tools, showing great potential for targeted genome editing, base editing, and genome regulation in plants. | Bin Ren Lang Liu Shaofang Li Yongjie Kuang Jingwen Wang Dawei Zhang Xueping Zhou Honghui Lin Huanbin Zhou | 2019 | Molecular Plant2019，12，7： | 18 |
| 3 | Synergistic immunoreaction of acupuncture-like dissolving microneedles containing thymopentin at acupoints in immune-suppressed rats显示文摘Dissolving microneedles carried drug molecules can effectively penetrate the stratum corneum of skin to improve the transdermal drug delivery. The traditional Chinese medicine acupuncture is based on the needle stimulation at a specific location(acupoint) to generate and transmit biochemical and physiological signals which alter the pathophysiological state of patients. However, the pain associated with conventional acupuncture needles and the requirement of highly trained professionals limit the development of acupuncture in non-Asian countries. The purpose of this study is to investigate whether the dissolving microneedles can be utilized as a self-administered painless replacement for acupuncture and locally released drug molecules can achieve expected therapeutic outcomes. Immunosuppressive rats were treated with acupuncture at Zusanli(ST36) acupoint using microneedles containing thymopentin.The immune functions and psychological mood of the immunosuppressed animals were examined. The proliferation of splenocytes was examined by CCK-8 assay. CD4 and CD8 expression patterns in spleen cells were detected by flow cytometry. The current study showed that use of either microneedles containing thymopentin or conventional acupuncture both resulted in immune cell proliferation, which was confirmed by flow cytometry. Furthermore, either conventional acupuncture or microneedles were able to effectively mitigate the anxiety caused by immune-suppression when applied on the ST36. | Qian Zhang Chuncao Xu Shiqi Lin Huanbin Zhou Gangtao Yao Hu Liu Lili Wang Xin Pan Guilan Quan Chuanbin Wu | 2018 | Acta Pharmaceutica Sinica B2018，8，3： | 9 |
| 4 | High-efficiency and multiplex adenine base editing in plants using new TadA variants显示文摘Recently reported adenine base editors(ABEs)exhibit powerful potential for targeted gene correction as well as developing gain-of-function mutants and novel germplasms for both gene function studies and crop breeding.However,editing efficiency varies significantly among different target sites.Here,we investigated the activities of three evolved E.coli adenosine deaminase TadA variants(TadA8e,TadA8.17,and TadA8.20)side-by-side in transgenic rice.We found that TadA8e outperforms TadA8.17 and TadA8.20,and induces efficient A-to-G conversion at all tested sites in the rice genome,including those that were un-editable by ABE7.10 in our previous experiments.Furthermore,V82S/Q154R mutations were incorporated into TadA8e,resulting in a new variant that we named TadA9.Our data show that TadA9 is broadly compatible with CRISPR/SpCas9,CRISPR/SpCas9-NG,and CRISPR/SpRY,as well as CRISPR/ScCas9 nickase systems,achieving comparable or enhanced editing in a larger editing window at diverse PAM sites as compared with TadA8e.Finally,TadA9 was used to simultaneously install novel SNPs in four endogenous herbicide target genes in the commercial rice cultivar Nangeng 46 for potential field application in.weed control.Collectively,we successfully generated a series of novel ABEs that can efficiently edit adenosines in the rice genome.Our findings suggest that TadA9 and TadA8e have great potentials in the development of plant base editors and crop molecular breeding. | Daqi Yan Bin Ren Lang Liu Fang Yan Shaofang Li Guirong Wang Wenxian Sun Xueping Zhou Huanbin Zhou | 2021 | Molecular Plant2021，14，5： | 8 |
| 5 | An investigation into the three-dimensional cloud structure over East Asia from the CALIPSO-GOCCP Data显示文摘The spatial distribution of clouds and their seasonal variations, and the three-dimensional(3D) cloud structures over East Asia have been analyzed with the CALIPSO-GOCCP data during the period from 2007 to 2012. The results show that there is a large cloud fraction greater than 0.7 over southern China, and the largest cloud fraction appears in southwest China. Besides, a large cloud fraction occurs over the southeast of the Tibetan Plateau. The total and high cloud fractions show notable variations with seasons, while the middle and low cloud fractions vary a little. As for cloud vertical structure, significant differences of the cloud vertical distributions are observed between over land and ocean. Cloud fractions and the height of the maximum cloud fractions decline gradually with the increasing latitude, except for the vertical-latitude profiles over the Tibetan Plateau regions. The longitude-vertical cross sections show similar patterns from the longitude 70° E to 140° E, except the profiles with large cloud fractions over the Tibetan Plateau. From the horizontal distribution patterns and vertical structures of the clouds over East Asia, it is concluded that the huge terrain of the Tibetan Plateau has significant impacts on the cloud formation over the Tibetan Plateau region and the areas to the east. At last, the clouds from the CALIPSO-GOCCP observations are compared to those from the ERA-Interim reanalysis data. The results indicate that the ERA-Interim reanalysis data provide reasonable spatial distribution patterns and the vertical structures in terms of the total cloud fraction over East Asia. However, the total cloud fraction was underestimated about 20% by the ERA-Interim reanalysis data over most parts of East Asia, especially over the neighboring areas east of the Tibetan Plateau. Additionally, the ERA-interim reanalysis data overestimate the cloud fractions at each level in the vertical direction. | YIN JinFang WANG DongHai XU HuanBin ZHAI GuoQing | 2015 | Science China Earth Sciences2015，58，12： | 4 |
| 6 | Bone marrow and the control of immunity显示文摘Bone marrow is thought to be a primary hematopoietic organ. However, accumulated evidences demonstrate that active function and trafficking of immune cells, including regulatory T cells, conventional T cells, B cells, dendritic cells, natural killer T (NKT) cells, neutrophils, myeloid-derived suppressor cells and mesenchymal stem cells, are observed in the bone marrow. Furthermore, bone marrow is a predetermined metastatic location for multiple human tumors. In this review, we discuss the immune network in the bone marrow. We suggest that bone marrow is an immune regulatory organ capable of fine tuning immunity and may be a potential therapeutic target for immunotherapy and immune vaccination. | Ende Zhao Huanbin Xu Lin Wang Ilona Kryczek Ke Wu Yu Hu Guobin Wang Weiping Zou | 2012 | Cellular & Molecular Immunology2012，9，1： | 3 |
| 7 | The rice codebook: From reading to editing显示文摘Rice(Oryza sativa)is the main staple food crop that feeds more than half of the world's population.Given its relatively small genome size,abundant genetic resources,and highly efficient transformation technology,rice also remains as an excellent model system for monocot crops.In 2002,the International Rice Genome Sequencing Project(IRGSP)released the draft genome sequence of rice variety Nipponbare,the first crop genetic codebook deciphered right after the human genome(Yu et al.,2002).Since then,rice genome annotation,large-scale genome resequencing of diverse rice germplasm accessions,a comprehensive map of rice genome variations,and high-quality reference genome sequence,especially the gap-free reference genome,were completed successively in the next two decades along with the development of high-throughput DNA sequencing techniques(Wang et al.,2018;Li et al.,2021;Song et al.,2021).Consequently,rice functional genomics research has boomed rapidly,greatly reshaping our knowledge of plant molecular biology and designed crop breeding. | Kejian Wang Huanbin Zhou Qian Qian | 2022 | Molecular Plant2022，15，4： | 1 |
| 8 | Nitric oxide contributes to the regulation of iron metabolism in skeletal muscle in vivo and in vitro显示文摘 | Haitao Wang Xianglin Duan Jianguo Liu Huanbin Zhao Yuqian Liu Yanzhong Chang | 2010 | Molecular and Cellular Biochemistry (-)2010，，1： | 1 |
| 9 | The E3 ubiquitin ligase OsRGLG5 targeted by the Magnaporthe oryzae effector AvrPi9 confers basal resistance against rice blast显示文摘Rice blast,caused by Magnaporthe oryzae,is one of the most devastating diseases of rice.During infection,M.oryzae secretes effectors to facilitate blast development.Among these effectors,the avirulence factor AvrPi9 is recognized by Pi9,a broad-spectrum blast resistance protein that triggers Pi9-mediated resistance in rice.However,little is known about the interaction between AvrPi9 and Pi9 and how AvrPi9 exerts virulence to promote infection.In this study,we found that ectopic expression of AvrPi9 in the Pi9-lacking cultivar TP309 suppressed basal resistance against M.oryzae.Furthermore,we identified an AvrPi9-interacting protein in rice,which we named OsRGLG5,encoding a functional RING-type E3 ubiquitin ligase.During infection,AvrPi9 was ubiquitinated and degraded by OsRGLG5.Meanwhile,AvrPi9 affected the stability of OsRGLG5.Infection assays revealed that OsRGLG5 is a positive regulator of basal resistance against M.oryzae,but it is not essential for Pi9-mediated blast resistance in rice.In conclusion,our results revealed that OsRGLG5 is targeted by the M.oryzae effector AvrPi9 and positively regulates basal resistance against rice blast. | Zhiquan Liu Jiehua Qiu Zhenan Shen Congcong Wang Nan Jiang Huanbin Shi Yanjun Kou | 2023 | Plant Communications2023，4，5： | 1 |
| 10 | Co-evolved plant and blast fungus ascorbate oxidases orchestrate the redox state of host apoplast to modulate rice immunity显示文摘Apoplastic ascorbate oxidases(AOs)play a critical role in reactive oxygen species(RoS)-mediated innate host immunity by regulating the apoplast redox state.To date,little is known about how apoplastic effectors of the riceblast fungus Magnaportheoryzaemodulate the apoplast redox state of rice to subvert plant immunity.In this study,we demonstrated that M.oryzae MoAo1 is an Ao that plays a role in virulence by modulating the apoplast redox status of rice cells.We showed that MoAo1 inhibits the activity of rice OsAO3and OsAO4,which also regulate the apoplast redox status and plant immunity.In addition,we found that MoAo1,OsAO3,andOsAO4 allexhibit polymorphic variations whosevaried interactions orchestrate pathogen virulence and rice immunity.Taken together,our results reveal a critical role for extracellular redox enzymes during rice blast infection and shed light on the importance of the apoplast redox state anditsregulation inplant-pathogeninteractions. | Jiexiong Hu Muxing Liu Ao Zhang Ying Dai Weizhong Chen Fang Chen Wenya Wang Danyu Shen Mary Jeanie Telebanco-Yanoria Bin Ren Haifeng Zhang Huanbin Zhou Bo Zhou Ping Wang Zhengguang Zhang | 2022 | Molecular Plant2022，15，8： | 0 |
| 11 | A Modified Double-Moment Bulk Microphysics Scheme Geared toward the East Asian Monsoon Region显示文摘Representation of cloud microphysical processes is one of the key aspects of numerical models.An improved double-moment bulk cloud microphysics scheme(named IMY)was created based on the standard Milbrandt-Yau(MY)scheme in the Weather Research and Forecasting(WRF)model for the East Asian monsoon region(EAMR).In the IMY scheme,the shape parameters of raindrops,snow particles,and cloud droplet size distributions are variables instead of fixed constants.Specifically,the shape parameters of raindrop and snow size distributions are diagnosed from their respective shape-slope relationships.The shape parameter for the cloud droplet size distribution depends on the total cloud droplet number concentration.In addition,a series of minor improvements involving detailed cloud processes have also been incorporated.The improved scheme was coupled into the WRF model and tested on two heavy rainfall cases over the EAMR.The IMY scheme is shown to reproduce the overall spatial distribution of rainfall and its temporal evolution,evidenced by comparing the modeled results with surface gauge observations.The simulations also successfully capture the cloud features by using satellite and ground-based radar observations as a reference.The IMY has yielded simulation results on the case studies that were comparable,and in ways superior to MY,indicating that the improved scheme shows promise.Although the simulations demonstrated a positive performance evaluation for the IMY scheme,continued experiments are required to further validate the scheme with different weather events. | Jinfang YIN Donghai WANG Guoqing ZHAI Hong WANG Huanbin XU Chongjian LIU | 2022 | Advances in Atmospheric Sciences2022，39，9： | 0 |
| 12 | JYPB2.0—A new composition adjust-ment processing system of metal melting显示文摘The new system contains all the independent variables, setting the ultimate objective function groups,installing the complete operating program chain and establishing the accurate mathematical model. The new systemproduces the ultimate objective function group- Optimum Proportion Scheme through the processing of the elevenoperating main programs such as Optimization Program, etc. and revising of three operating auxiliary programssuch as Elimination Program, etc. which is locked by the intermediary objective function group. The setting of thecomplete database system provides the full-service for the customers' smelting production. It is generally applicablefor all the varieties of alloys and raw materials. Its customized features can fulfill the actual needs of users. These arethe advantages of the new composition adjustment processing system of metal melting- JYPB2.0. | Wei Wenchao Wang Zhongce Wang Huanbin | 2008 | China Foundry2008，5，1： | 0 |
| 13 | ASAP3 regulates microvilli structure in parietal cells and presents intervention target for gastric acidity显示文摘Gastric acidity-associated disorders such as peptic ulcer and reflux diseases are widespread,and the reported resistance and side effects of currently used medicines suggest an urgent requirement for alternative therapeutic approaches.Here we demonstrate a critical role of ASAP3 in regulating the microvilli structure of parietal cells in vivo,and reveal the feasibility of controlling gastric acidity by targeting ASAP3.Conditional knockout of ASAP3 in mice caused elongation and stacking of microvilli in parietal cells,and substantially decreased gastric acid secretion.These were associated with active assembly of F-actin caused by a higher level of GTP-bound Arf6 GTPase.Consistently,a small molecular compound QS11 inhibited ASAP3 function and significantly reduced gastric acidity in vivo.Of note,the expression of ASAP3 was positively correlated with gastric acid secretion in 90 human cases,and high expression of ASAP3 was associated with reflux disease and peptic ulcer.These results reveal for the first time that ASAP3 regulates the microvilli structures in parietal cells.Our data also suggest ASAP3 as a feasible and drugable therapeutic target for gastric acidity-associated diseases. | Jin Qian Yueyuan Li Han Yao Haiying Tian Huanbin Wang Luoyan Ai Yuanhong Xie Yujie Bao Lunxi Liang Ye Hu Yao Zhang Jilin Wang Chushu Li Jiayin Tang Yingxuan Chen Jie Xu Jing-Yuan Fang | 2017 | Signal Transduction and Targeted Therapy2017，2，1： | 0 |
| 14 | Three novel alleles of OsGS1 developed by base-editing-mediated artificial evolution confer glufosinate tolerance in rice显示文摘Only few glufosinate-tolerant genes,such as phosphinothricin acetyltransferase(PAT)and bialaphos resistance(bar)identified from Streptomyces,are currently available for developing genetically modified rice in agricultural application.Following the rapid development of genome editing technology,generation of novel glufosinate-tolerant gene resources through artificial evolution of endogenous genes is more promising and highly desirable in rice molecular breeding program.In this study,the endogenous Glutamine synthetase1(OsGS1)was artificially evolved by base-editing-mediated gene evolution(BEMGE)in rice cells to create novel alleles conferring glufosinate tolerance in rice germplasms.Two novel glufosinate-tolerant OsGS1 alleles(OsGS1-AVPS and OsGS1-+AF)and one reported tolerant allele(OsGS1-SGTA)were successfully identified from approximately 4200 independent hygromycin-tolerant calli.Germination assays and spray tests revealed that these three OsGS1 alleles confer glufosinate tolerance in rice.Furthermore,OsGS1-AVPS and OsGS1-SGTA were quickly deployed into the elite rice cultivar Nangeng 46 through precise base editing.Overall,our results demonstrate the feasibility of developing glufosinate-tolerant rice by editing an endogenous rice gene in molecular breeding programs. | Bin Ren Yongjie Kuang Ziyan Xu Xuemei Wu Dawei Zhang Fang Yan Xiangju Li Xueping Zhou Guirong Wang Huanbin Zhou | 2023 | The Crop Journal2023，11，2： | 0 |
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