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| 1 | Adoptive cell transfer therapy for hepatocellular carcinoma显示文摘Hepatocellular carcinoma (HCC) is one of the most common cancers worldwide. This malignancy is associated with poor prognosis and high mortality. Novel approaches for prolonging the overall survival of patients with advanced HCC are urgently needed. The antitumor activities of adoptive cell transfer therapy (ACT), such as strategies based on tumor-infiltrating lymphocytes and cytokine-induced killer cells, are more effective than those of traditional strategies. Currently, chimeric antigen receptor T-cell (CAR-T) immunotherapy has achieved numerous breakthroughs in the treatment of hematological malignancies, including relapsed or refractory lymphoblastic leukemia and refractory large B-cell lymphoma? Nevertheless, this approach only provides a modest benefit in the treatment of solid tumors. The clinical results of CAR-T immunotherapy for HCC that could be obtained at present are limited. Some published studies have demonstrated that CAR-T could inhibit tumor growth and cause severe side effects. In this review, we summarized the current application of ACT, the challenges encountered by CAR-T technology in HCC treatment, and some possible strategies for the future direction of immunotherapeutic research. | Renyu Zhang Zhao Zhang Zekun Liu Ding Wei Xiaodong Wu Huijie Bian Zhinan Chen | 2019 | Frontiers of Medicine2019，13，1： | 17 |
| 2 | Chimeric antigen receptor T cell targeting EGFRvIII for metastatic lung cancer therapy显示文摘Lung cancer is the most common incident cancer and the leading cause of cancer death. In recent years, the development of tumor immunotherapy especially chimeric antigen receptor T (CAR-T) cell has shown a promising future. Epidermal growth factor receptor variant III (EGFRvlll) is a tumor-specific mutation expressed in various types of tumors and has been detected in non-small cell lung cancer with a mutation rate of 10%. Thus, EGFRvIII is a potential antigen for targeted lung cancer therapy. In this study, CAR vectors were constructed and transfected into virus-packaging cells. Then, activated T cells were infected with retrovirus harvested from stable virus-producing single clone cell lines. CAR expression on the surfaces of the T cells was detected by flow cytometry and Western blot. The function of CAR-T targeting EGFRvIII was then evaluated. The EGFRvIII-CAR vector was successfully constructed and confirmed by DNA sequencing. A stable virus-producing cell line was produced from a single clone by limited dilution. The culture conditions for the cell line, including cell density, temperature, and culture medium were optimized. After infection with retrovirus, CAR was expressed on more than 90% of the T cells. The proliferation of CAR-T cells were induced by cytokine and specific antigen in vitro. More importantly, EGFRvIII-CART specifically and efficiently recognized and killed A549-EGFRvIII cells with an effector/target ratio of 10:1 by expressing and releasing cytokines, including perforin, granzyme B, IFN-γ, and TNF-α. The in vivo study indicated that the metastasis of A549-EGFRvIII cells in mice were inhibited by EGFRvIII-CART cells, and the survival of the mice was significantly prolonged with no serious side effects. EGFRvIII-CART showed significantly efficient antitumor activity against lung cancer cells expressing EGFRvlll in vivo and in vitro. Therefore, CAR-T targeting EGFRvIII is a potential therapeutic strategy in preventing recurrence and metastasis of lung cancer after surgery. | Zhao Zhang Jun Jiang Xiaodong Wu Mengyao Zhang Dan Luo Renyu Zhang Shiyou Li Youwen He Huijie Bian Zhinan Chen | 2019 | Frontiers of Medicine2019，13，1： | 11 |
| 3 | CD147-spike protein is a novel route for SARS-CoV-2 infection to host cells显示文摘In face of the everlasting battle toward COVID-19 and the rapid evolution of SARS-CoV-2,no specific and effective drugs for treating this disease have been reported until today.Angiotensin-converting enzyme 2(ACE2),a receptor of SARS-CoV-2,mediates the virus infection by binding to spike protein.Although ACE2 is expressed in the lung,kidney,and intestine,its expressing levels are rather low,especially in the lung.Considering the great infectivity of COVID-19,we speculate that SARS-CoV-2 may depend on other routes to facilitate its infection.Here,we first discover an interaction between host cell receptor CD147 and SARS-CoV-2 spike protein.The loss of CD147 or blocking CD147 in Vero E6 and BEAS-2B cell lines by anti-CD147 antibody,Meplazumab,inhibits SARSCoV-2 amplification.Expression of human CD147 allows virus entry into non-susceptible BHK-21 cells,which can be neutralized by CD147 extracellular fragment.Viral loads are detectable in the lungs of human CD147(hCD147)mice infected with SARS-CoV-2,but not in those of virus-infected wild type mice.Interestingly,virions are observed in lymphocytes of lung tissue from a COVID-19 patient.Human T cells with a property of ACE2 natural deficiency can be infected with SARS-CoV-2 pseudovirus in a dosedependent manner,which is specifically inhibited by Meplazumab.Furthermore,CD147 mediates virus entering host cells by endocytosis.Together,our study reveals a novel virus entry route,CD147-spike protein,which provides an important target for developing specific and effective drug against COVID-19. | Ke Wang Wei Chen Zheng Zhang Yongqiang Deng Jian-Qi Lian Peng Du Ding Wei Yang Zhang Xiu-Xuan Sun Li Gong Xu Yang Lei He Lei Zhang Zhiwei Yang Jie-Jie Geng Ruo Chen Hai Zhang Bin Wang Yu-Meng Zhu Gang Nan Jian-Li Jiang Ling Li Jiao Wu Peng Lin Wan Huang Liangzhi Xie Zhao-Hui Zheng Kui Zhang Jin-Lin Miao Hong-Yong Cui Min Huang Jun Zhang Ling Fu Xiang-Min Yang Zhongpeng Zhao Shihui Sun Hongjing Gu Zhe Wang Chun-Fu Wang Yacheng Lu Ying-Ying Liu Qing-Yi Wang Huijie Bian Ping Zhu Zhi-Nan Chen | 2020 | Signal Transduction and Targeted Therapy2020，5，1： | 9 |
| 4 | A drainage-enhancing device for foam fractionation of proteins显示文摘The foam fractionation of nisin from its fermentation broth was studied.Two types of devices consisting of a rubber piston and a foam riser were developed to enhance foam drainage.The separation performance of these two devices was investigated.Experimental results indicated that the second device could significantly reduce the liquid fraction of the foam leaving the column,εout,leading to a higher enrichment of the out-flow stream.As its mounting height increased from 0 to 15 cm,εout declined from 7.07‰ to 6.13 ‰ and the maximum nisin activity in the foamate could reach 39.6 IU/μL.The slight increase in nisin inactivation rate indicated the applicability of this method in the recovery and concentration of proteins.Finally,the mechanism of the process was primarily explained by invoking recent work on pneumatic foams.This research provides a basis for the design of multistage draining foam fractionator which could potentially be an effective separation equipment. | WU ZhaoLiang QIAN ShaoYu ZHENG HuiJie ZHAO YanLi | 2010 | Chinese Science Bulletin2010，55，12： | 7 |
| 5 | A Positive Mutual Impact between Inclusive Finance and Poverty Alleviation显示文摘The contradiction between profitability requirement of commercial finance and non-profitability nature of inclusive finance inhibits bankers' motivation in providing financial services to farmers,further restricting the already narrow financing channels for low-income rural households and preventing them from jumping out of the poverty trap.There is a mutual promotion relationship between inclusive finance and poverty reduction.On one hand,providing affordable and subsidized financial services to farmers can help them increase income steadily; On the other hand,the institutions reap a good payoff by business expansion,a thick base of loyal clients,as well as public recognition,which in turn stimulates the institutes to provide more financial products,improve their service quality and scale up the investment amount for lowincome farmers.Nevertheless,to set off this positive feedback loop process,a forcible intervention by government is needed.On the base of theoretical analysis and practical research,this article brought related advice for financial institutions in order to promote the development of inclusive finance and alleviate the financing difficulties of farmers households to realize poverty alleviation. | Huijie ZHAO Luochuan CHEN | 2019 | Asian Agricultural Research2019，11，1： | 7 |
| 6 | miR-375 is highly expressed and possibly transactivated by achaete-scute complex homolog 1 in small-cell lung cancer cells显示文摘在这研究,我们鉴别五 miRNAs 高度在小房间的肺癌症(SCLC ) 表示了房间线 NCI-H209。在他们之中, miR-375 的表示层次戏剧性地在检验的所有 SCLC 房间线被提高,与抄写因素 achaete 鳞甲建筑群相当或相同的事物的表示重合 1 (ASCL1 ) 。而且, miR-375 是 upregulated 并且在也从老鼠象 SCLC 一样肿瘤产生的房间线与 ASCL1 相关。双酶的试金进一步证明 ASCL1 在 miR-375 倡导者由绑定激活 miR-375 的表达式到三个电子盒子元素。这些结果经由 miR-375 的 upregulation 在 SCLC 暗示 ASCL1 的一个角色。 | Huijie Zhao Lei Zhu Yujuan Jin Hongbin Ji Xiumin Yan Xueliang Zhu | 2012 | Acta Biochimica et Biophysica Sinica2012，44，2： | 6 |
| 7 | Facile one-step synthesis of Cu_2O@Cu sub-microspheres composites as anode materials for lithium ion batteries显示文摘Cu_2O@Cu sub-microspheres composites with a narrow particle size distribution from 300 to 500 nm was successfully fabricated by one-step synthesis through the direct thermal decomposition of copper nitrate(Cu(NO_3)_2) in octadecylamine(ODA) solvent. As anode materials for lithium ion batteries, the Cu_2 O@Cu composites obviously possess high specific capacity, excellent cyclic stability and rate capability. The coulombic efficiency is about 84% in the 1 st cycle and increases significantly up to 97.8% during successive cycles at various current densities. Even under a high current density of 500 m A g^(-1), the discharge capacity of Cu_2 O@Cu composites remains up to 200 m Ah g^(-1). The excellent electrochemical properties are ascribed to the synergistic effect between high electronic conductivity and volume-buffering capacity of metallic copper composited with Cu_2 O. | Huijie Zhou Hongbin Zhao Xuan Zhang Hongwei Cheng Xionggang Lu Qian Xu | 2018 | Journal of Materials Science & Technology2018，34，7： | 3 |
| 8 | Improved Multi-objective Ant Colony Optimization Algorithm and Its Application in Complex Reasoning显示文摘The problem of fault reasoning has aroused great concern in scientific and engineering fields.However,fault investigation and reasoning of complex system is not a simple reasoning decision-making problem.It has become a typical multi-constraint and multi-objective reticulate optimization decision-making problem under many influencing factors and constraints.So far,little research has been carried out in this field.This paper transforms the fault reasoning problem of complex system into a paths-searching problem starting from known symptoms to fault causes.Three optimization objectives are considered simultaneously: maximum probability of average fault,maximum average importance,and minimum average complexity of test.Under the constraints of both known symptoms and the causal relationship among different components,a multi-objective optimization mathematical model is set up,taking minimizing cost of fault reasoning as the target function.Since the problem is non-deterministic polynomial-hard(NP-hard),a modified multi-objective ant colony algorithm is proposed,in which a reachability matrix is set up to constrain the feasible search nodes of the ants and a new pseudo-random-proportional rule and a pheromone adjustment mechinism are constructed to balance conflicts between the optimization objectives.At last,a Pareto optimal set is acquired.Evaluation functions based on validity and tendency of reasoning paths are defined to optimize noninferior set,through which the final fault causes can be identified according to decision-making demands,thus realize fault reasoning of the multi-constraint and multi-objective complex system.Reasoning results demonstrate that the improved multi-objective ant colony optimization(IMACO) can realize reasoning and locating fault positions precisely by solving the multi-objective fault diagnosis model,which provides a new method to solve the problem of multi-constraint and multi-objective fault diagnosis and reasoning of complex system. | WANG Xinqing ZHAO Yang WANG Dong ZHU Huijie ZHANG Qing | 2013 | Chinese Journal of Mechanical Engineering2013，26，5： | 3 |
| 9 | A NOVEL COMPACT HIS-EBG STRUCTURE AND ITS APPLICATION IN REDUCTION OF PRINTED ANTENNA MUTUAL COUPLING显示文摘Anovel compact E shape High Impedance Surface(HIS) Electromagnetic Band-Gap(EBG) comprising Amended Minkowski Fractal Boundary(AMFB),Added Metal Strips(AMS),and metal plated via printed on a Rogers TMM 10i substrate is presented.The first order AMFB and ABS are introduced for equivalent capacitance enhancement and miniaturization with effective space-filling characteristics.The dimension of lattice size is only The proposed structure exhibits a most compact characteristic about 35% size reduction as compared with conven-tional mushroom-like HIS-EBG,and better surface-wave decoupling of around 9 dB at the operating frequency band.Details of design parameters which affect the whole performance of certain band-gap are intensively investigated.A constructed prototype is potential candidate for antenna system per-formance enhancement. | Lv Yuan Zhao Xingwei Liu Huijie Shen Xuemin | 2011 | Journal of Electronics(China)2011，28，2： | 2 |
| 10 | Thrombin is a therapeutic target for non-small-cell lung cancer to inhibit vasculogenic mimicry formation显示文摘Tumor cells transform into endothelial cells by epithelial-to-mesenchymal transition,which is characterized by vasculogenic mimicry(VM).VM not only accelerates tumor progression but also increases drug-induced resistance.However,very little is currently known about the molecular determinants that enable VM.Targeting VM might bring a new breakthrough in cancer treatment.Thrombin is the key enzyme of the blood coagulation system and could contribute to tumor progression.Nevertheless,the association between thrombin and VM formation remains largely unknown.We found that VM was associated with the overall survival of non-small-cell lung cancer(NSCLC)patients,and that thrombin expression was closely related to VM formation.This research revealed that thrombin induced VM formation via PAR-1-mediated NF-κB signaling cascades.The novel thrombin inhibitors r-hirudin and DTIP inhibited VM formation and spontaneous metastases in subcutaneous tumors.Clinical pathological analysis confirmed that NSCLC patients with thrombin-positive/PAR-1-high expression had the poorest prognosis and were the most likely to form VM.The promotional activity of thrombin in VM formation and tumor metastasis was abolished in PAR-1-deficient NSCLC cells.The EGFR inhibitor gefitinib had no effect on VM and increased VEGF expression in tumors.The combination therapy of DTIP and gefitinib achieved a better therapeutic effect than either agent alone.This study is the first to illustrate that thrombin substantially contributes,together with PAR-1,to VM formation and to illustrate that VM might be a target of r-hirudin and DTIP to suppress tumor progression.The anticoagulants r-hirudin and DTIP could be employed for antitumor therapy.Combination therapy with DTIP with an EGFR inhibitor might achieve superior therapeutic effects. | Bing Zhao Mengfang Wu Zhihuang Hu Yixin Ma Wang Qi Yanling Zhang Yaran Li Min Yu Huijie Wang Wei Mo | 2020 | Signal Transduction and Targeted Therapy2020，5，1： | 2 |
| 11 | A spatio-velocity model based semantic event detection algorithm for traffic surveillance video显示文摘Detection of vehicle events is a research hotspot in digital traffic.In this paper,an approach is proposed to detect vehicle events with semantic analysis of traffic surveillance video using spatio-velocity statistic models.The approach includes two successive phases:trajectory clustering and semantic events detection.For trajectory clustering,a statistic model of vehicle trajectories are presented,for which a spatio-velocity model is trained by analyzing the trajectories of moving vehicles in the scene.Based on the trajectory,which represents both the position of the vehicle and its instantaneous velocity,a trajectory similarity measure is proposed.Then,an improved hierarchical clustering algorithm is adopted to cluster the trajectories according to different spatial and velocity distributions.In each cluster,trajectories that are spatially close have similar velocities of motion and represent one type of activity pattern.For the semantic events detection phase,statistic models of semantic regions in the scene are generated by estimating the probability density and velocity distributions of each type of activity pattern.Finally,semantic events are detected by the proposed spatio-velocity statistic models.The paper also presents experiments using real video sequence to verify the effectiveness of the proposed method. | SHENG Hao 1,ZHAO HuiJie 1,HUANG Jian 2 & LI Na 1 1 School of Instrument Science and Opto-Electro Engineering,Beihang University,Beijing 100191,China 2 School of Software,Beihang University,Beijing 100191,China | 2010 | Science China(Technological Sciences)2010，53，S1： | 2 |
| 12 | Laser Scan- ning Based Three Dimensional Measurement of Vegeta- tion Canopy Structure 显示文摘 | LI Xudong ZHAO Huijie LIU Yang | 2014 | Optics and Lasers in Engi- neering2014，54，1： | 1 |
| 13 | Modeling SARS Spreading on Complex Networks显示文摘 | Yang Huijie Zhao Fangcui Li Zhongnan Hu Beilai | 2004 | International Journal of Modern Physics B2004，，18： | 1 |
| 14 | Synthesis,characterization,and application of hydotalcites in hydrodesulfurization of FCC gasoline 显示文摘 | Zhao Ruiyu Yin Changlong Zhao Huiji | 2003 | Fuel Processing Technology2003，81，3： | 1 |
| 15 | Tri- pod - like Compounds : Syntheses of tris ( p - OR o - Amino Phe- noxymethyl) ,tris( p - OR o - Amino Phcnoxyethyl) Amine, and their Schiff Base or Salicyl Derivatives 显示文摘 | Huijie Lu Yaoting Fan Qinghuan Zhao and Yangjie Wu | 2001 | Synthetic Communica- tions2001，31，10： | 1 |
| 16 | Acceleration-Dependent Analysis of Vertical Ball Screw Feed System without Counterweight显示文摘The distinguishing feature of a vertical ball screw feed system without counterweight is that the spindle system weight directly acts on the kinematic joints.Research into the dynamic characteristics under acceleration and deceleration is an important step in improving the structural performance of vertical milling machines.The magnitude and direction of the inertial force change significantly when the spindle system accelerates and decelerates.Therefore,the kinematic joint contact stiffness changes under the action of the inertial force and the spindle system weight.Thus,the system transmission stiffness also varies and affects the dynamics.In this study,a variable-coefficient lumped parameter dynamic model that considers the changes in the spindle system weight and the magnitude and direction of the inertial force is established for a ball screw feed system without counterweight.In addition,a calculation method for the system stiffness is provided.Experiments on a vertical ball screw feed system under acceleration and deceleration with different accelerations are also performed to verify the proposed dynamic model.Finally,the influence of the spindle system position,the rated dynamic load of the screw-nut joint,and the screw tension force on the natural frequency of the vertical ball screw feed system under acceleration and deceleration are studied.The results show that the vertical ball screw feed system has obviously different variable dynamics under acceleration and deceleration.The influence of the rated dynamic load and the spindle system position on the natural frequency under acceleration and deceleration is much greater than that of the screw tension force. | Cunfan Zou Huijie Zhang Jun Zhang Dongdong Song Hui Liu Wanhua Zhao | 2021 | Chinese Journal of Mechanical Engineering2021，34，3： | 1 |
| 17 | Modeling SARS Spreading on Complex Networks 显示文摘 | Yang Huijie Zhao Fangcui Li Zhongnan | 2004 | International journal of Modem Pysics B (S0217-9792)2004，18，17： | 1 |
| 18 | Cable-Strengthened C2 Pedicle Screw Fixation in the Treatment of Congenital C2-3 Fusion, Atlas Occipitalization, and Atlantoaxial Dislocation显示文摘 | Shenglin Wang Chao Wang Huijie Leng Weidong Zhao Ming Yan Haitao Zhou | 2012 | Neurosurgery2012，，5： | 1 |
| 19 | The quantitative evaluation of application of hyperspectral data based on multi-parameters joint optimization显示文摘In order to evaluate the mineral identification of the hyperspectral data and make a trade-off of the imaging system parameters,a quantitative evaluation approach based on the multi-parameters joint optimization is proposed for the hyperspectral remote sensing.In the proposed approach,the mineral identification is defined as the number of the minerals identified and the key imaging parameters employed include ground sample distance(GSD)and spectral resolution(SR).Certain limitations are found among parameters that are used for analyzing the imaging processes.The constraints include the industrial manufacturing level,application requirements and the quantitative relationship among the GSD,the SR and the signal-to-noise ratio(SNR).Regression analysis is used to investigate the quantitative relationship between the mineral identification and the key imaging system parameters.Then,an optimization model for the trade-off study is established by combining the regression equation with the constraints.The airborne hyperspectral image collected by Hymap is applied to evaluate the performance of the proposed approach.The experimental results reveal that the approach can achieve the evaluation of the mineral identification and the trade-off of key imaging system parameters.The error of the prediction is within one kind of mineral. | LI Na HUANG Ping ZHAO HuiJie JIA GuoRui | 2014 | Science China(Technological Sciences)2014，57，11： | 1 |
| 20 | Synthesis, characterization, and application of hydotalcites in hydro- desulfurization of FCC gasoline 显示文摘 | Ruiyu Zhao Changlong Yin Huiji Zhao etal | 2003 | Fuel Processing Technology2003，81，： | 1 |
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