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| 1 | BE-PLUS: a new base editing tool with broadened editing window and enhanced fidelity显示文摘基础编辑() ,包含 cytidine 脱氨基酶和催化地有缺点的 Cas9,广泛地被用来执行基础编辑。然而,狭窄的编辑窗口是限制它的实用程序。这里,我们开发了依照到由熔化 10 的 U (T) 范围(加) 的更大的 C 为招募 GCN4 肽拷贝到 nCas9 (D10A ) 的编程作为基础编辑命名的一种新编辑技术 scFv-APOBEC-UGI-GB1 到目标地点。新系统完成与一扇拓宽的窗户的基础编辑,导致一个增加的指向染色体的范围。有趣地,新系统很产出更少讨厌的 indels 和 non-C-to-T 变换。我们也通过站 codons (iSTOP ) 的正式就职越过整个染色体在基因混乱表明了它的潜在的使用。一起拿,加系统与编辑窗口和提高的忠实增加提供一个新编辑工具。 | Wen Jiang Songjie Feng Shisheng Huang Wenxia Yu Guanglei Li Guang Yang Yajing Liu Yu Zhang Lei Zhang Yu Hou Jia Chen Jieping Chen Xingxu Huang | 2018 | Cell Research2018，28，8： | 20 |
| 2 | PTEN基因甲基化及其表达异常与胃癌的关系(英文)显示文摘Objective:To probe into the relationships between PTEN gene expression,the promoter methylation and gastric cancer and its clinical pathological specific features.Methods:We analyzed the PTEN gene promoter methylation and mRNA expression status in gastric cancer tissues and its adjacent normal tissues by methylation specific PCR(MSP) and reverse transcription-polymerase chain reaction(RT-PCR) techniques.Results:PTEN promoters in 48.2%(27/56) gastric cancer tissues and 3.6(2/56) adjacent normal tissues were methylated and the PTEN promoter methylation rate in carcinoma tissues was obviously higher(P < 0.05).Of the 2 cases where the adjacent gastric tissues were methylated,the gastric cancer tissues were both methylated.Of the 29 gastric cancers with lymph node metastasis,19 had their PTEN gene promoters methylated and the PTEN gene promoter methylation in cases with lymph node metastasis was obviously higher than that without lymph node metastasis(P < 0.05).RT-PCR result showed that no expression of PTEN mRNA existed in any of the methylated gastric cancer tissues.Conclusion:The expression loss of PTEN gene mRNA in gastric cancers is related to their promoter methylation and might be one of the reasons for the generation,development and metastasis of gastric cancers. | Song Liu Jieping Yu Ping Fu Honggang Yu | 2008 | The Chinese-German Journal of Clinical Oncology2008，7，10： | 3 |
| 3 | Extra-copy nifA enhances the nodulation efficiency of Sinorhizobium fredii显示文摘Previous investigations have shown that nifA gene is involved in nodulation and symbiotic nitrogen fixation regulation of Rhizobium. We study the role of nifA on nodulation of leguminous plants. We found that Sinorhizo-bium fredii harboring multi-copy plasmid carrying the con-stitutively expressed Klebsiella pneumoniae nifA exhibited an increase of nodulation activity and nodulation competitiveness on soybean plants. The Nod-factor secreted by the rhizobia cells containing the multi-copied nifA was assayed, and preliminary results showed that S. fredii containing the multi-copy plasmid carrying nifA produced higher strength of Nod-factor than the rhizobia containing the same plasmid carrying the vector did. | Zhao Jieping Dai Xiaomi Xu Ling Zhu Jiabi Shen Shanjiong Yu Guanqiao | 2002 | Chinese Science Bulletin2002，47，7： | 2 |
| 4 | Protecting-Group-Free Total Synthesis and Biological Investigation of Cabucine Oxindole A显示文摘Owing to their challenging structures and promising biological profiles,spirooxindole alkaloids have long attracted much attention from the synthetic community.Herein,we wish to describe a concise,protecting-group-free total synthesis of cabucine oxindole A,a putative natural spirooxindole alkaloid and a possible biosynthetic congener of cabucine and palmirine.Key transformations of our approach include a one-step,organocatalytic and enantioselective construction of the spiro[pyrrolidine-3,3'-oxindole]moiety and a Korte rearrangement to furnish the final dihydropyran motif.Biological investigation of 1 and its synthetic intermediates revealed lactone 2 as a mild MOLT-4 and MCF7 cell line inhibitor. | Shengling Xie Chengqing Ning Qingzhen Yu Jieping Hou Jing Xu | 2021 | Chinese Journal of Chemistry2021，39，1： | 2 |
| 5 | Effects ofstearates on the thermal stability of chlorinated natural rubber from latex, Journal of Applied Polymer Science显示文摘 | Yu Heping Li Sidong Zhong Jieping | 2006 | 99(4): 1981-19852006，99，4： | 1 |
| 6 | A flexible method for image noise removal显示文摘 | Liu Jieping Yu Yinglin | 2000 | Joural of South China University Technology2000，28，2： | 1 |
| 7 | Chemoresistance to doxorubicin induces epithelial-mesenchymal transitionvia upregulation of transforming growth factor β signaling in HCT116 colon cancercells显示文摘 | Jinpeng Li Hao Liu Jieping Yu Honggang Yu | 2015 | Molecular Medicine Reports2015，，： | 1 |
| 8 | Research Progress of MOFs Materials in Separation Technology显示文摘Metal-organic frameworks(MOFs)are a class of porous crystalline materials,consisting of metal ions or clusters coordinated to organic ligands.It has the advantages of ultra-high specific surface area,diverse structure,good thermal stability,adjustable pore size and properties,and shows important application value in the field of separation.In this paper,the research progress of metal-organic framework materials in gas separation and chromatographic separation is reviewed,and the existing problems and development direction of metal-organic framework materials in adsorption and separation are discussed. | Yu LIN Jieping QIN Wen LIU Zhenzhen PAN Jian ZHANG | 2020 | Medicinal Plant2020，11，4： | 0 |
| 9 | Spatial-temporal characteristics of epidemic spread in-out flow——Using SARS epidemic in Beijing as a case study显示文摘For better detecting the spatial-temporal change mode of individual susceptible-infected-symptomatic-treated-recovered epidemic progress and the characteristics of information/material flow in the epidemic spread network between regions,the epidemic spread mechanism of virus input and output was explored based on individuals and spatial regions.Three typical spatial information parameters including working unit/address,onset location and reporting unit were selected and SARS epidemic spread in-out flow in Beijing was defined based on the SARS epidemiological investigation data in China from 2002 to 2003 while its epidemiological characteristics were discussed.Furthermore,by the methods of spatial-temporal statistical analysis and network characteristic analysis,spatial-temporal high-risk hotspots and network structure characteristics of Beijing outer in-out flow were explored,and spatial autocorrelation/heterogeneity,spatial-temporal evolutive rules and structure characteristics of the spread network of Beijing inner in-out flow were comprehensively analyzed.The results show that(1)The outer input flow of SARS epidemic in Beijing concentrated on Shanxi and Guangdong provinces,but the outer output flow was disperse and mainly includes several north provinces such as Guangdong and Shandong.And the control measurement should focus on the early and interim progress of SARS breakout.(2)The inner output cases had significant positive autocorrelative characteristics in the whole studied region,and the high-risk population was young and middle-aged people with ages from 20 to 60 and occupations of medicine and civilian labourer.(3)The downtown districts were main high-risk hotspots of SARS epidemic in Beijing,the northwest suburban districts/counties were secondary high-risk hotspots,and northeast suburban areas were relatively safe.(4)The district/county nodes in inner spread network showed small-world characteristics and information/material flow had notable heterogeneity.The suburban Tongzhou and Changping districts were the underlying high-risk regions,and several suburban districts such as Shunyi and Huairou were the relatively low-risk safe regions as they carried out minority information/material flow.The exploration and analysis based on epidemic spread in-out flow help better detect and discover the potential spatial-temporal evolutive rules and characteristics of SARS epidemic,and provide a more effective theoretical basis for emergency/control measurements and decision-making. | HU BiSong GONG JianHua ZHOU JiePing SUN Jun YANG LiYang XIA Yu Abdoul Nasser IBRAHIM | 2013 | Science China Earth Sciences2013，56，8： | 0 |
| 10 | Cell-autonomous effect of cardiomyocyte branched-chain amino acid catabolism in heart failure in mice显示文摘Parallel to major changes in fatty acid and glucose metabolism,defect in branched-chain amino acid(BCAA)catabolism has also been recognized as a metabolic hallmark and potential therapeutic target for heart failure.However,BCAA catabolic enzymes are ubiquitously expressed in all cell types and a systemic BCAA catabolic defect is also manifested in metabolic disorder associated with obesity and diabetes.Therefore,it remains to be determined the cell-autonomous impact of BCAA catabolic defect in cardiomyocytes in intact hearts independent from its potential global effects.In this study,we developed two mouse models.One is cardiomyocyte and temporal-specific inactivation of the E1αsubunit(BCKDHA-cKO)of the branched-chainα-ketoacid dehydrogenase(BCKDH)complex,which blocks BCAA catabolism.Another model is cardiomyocyte specific inactivation of the BCKDH kinase(BCKDK-cKO),which promotes BCAA catabolism by constitutively activating BCKDH activity in adult cardiomyocytes.Functional and molecular characterizations showed E1αinactivation in cardiomyocytes was sufficient to induce loss of cardiac function,systolic chamber dilation and pathological transcriptome reprogramming.On the other hand,inactivation of BCKDK in intact heart does not have an impact on baseline cardiac function or cardiac dysfunction under pressure overload.Our results for the first time established the cardiomyocyte cell autonomous role of BCAA catabolism in cardiac physiology.These mouse lines will serve as valuable model systems to investigate the underlying mechanisms of BCAA catabolic defect induced heart failure and to provide potential insights for BCAA targeted therapy. | Jia-yu Yu Nancy Cao Christoph DRau Ro-Po Lee Jieping Yang Rachel JRoth Flach Lauren Petersen Cansheng Zhu Yea-Lyn Pak Russell AMiller Yunxia Liu Yibin Wang Zhaoping Li Haipeng Sun Chen Gao | 2023 | Acta Pharmacologica Sinica2023，44，7： | 0 |
| 11 | Overcoming adaptive resistance in AML by synergistically targeting FOXO3A-GNG7-mTOR axis with FOXO3A inhibitor Gardenoside and rapamycin显示文摘Therapeutic targeting FOxO3A(a forkhead transcription factor)represents a prom-ising strategy to suppress acute myeloid leukemia(AML).However,the effective inhibitors that target FOXO3A are lacking and the adaptive response signaling weakens the cytotoxic effect of FOxO3A depletion on AML cells.Here,we show that FOxO3A deficiency induces a compensa-tory response involved in the reactive activation of mTOR that leads to signaling rebound and adaptive resistance.Mitochondrial metabolism acts downstream of mTOR to provoke activa-tion of JNK/c-JUN via reactive oxygen species(ROS).At the molecular level,FOXO3A directly binds to the promoter of G protein gamma subunit 7(GNG7)and preserves its expression,while GNG7 interacts with mTOR and restricts phosphorylated activation of mTOR.Consequently,combinatorial inhibition of FOXO3A and mTOR show a synergistic cytotoxic effect on AML cells and prolongs survival in a mouse model of AML.Through a structure-based virtual screening,we report one potent small-molecule FOxO3A inhibitor(Gardenoside)that exhibits a strong effect of anti-FOXO3A DNA binding.Gardenoside synergizes with rapamycin to substantially reduce tumor burden and extend survival in AML patient-derived xenograft model.These results demonstrate that mTOR can mediate adaptive resistance to FOxO3A inhibition and validate a combinatorial approach for treating AML. | Zhe Chen Qian Guo Shichen Huang Lei Li Feng Wu Zhilong Liu Zhigang Li Tao Chen Guanbin Song Shuangnian Xu Jieping Chen Yu Hou | 2024 | Genes & Diseases2024，11，1： | 0 |
| 12 | Are the SNPs of NKX2-1 associated with papillary thyroid carcinoma in the Han population of Northern China?显示文摘乳突的甲状腺癌(PTC ) 是甲状腺腺的最普通的肿瘤之一。PTC 的普通风险因素包括电离放射,积极家庭历史,和甲状腺榴状的疾病。PTC 被进行染色体宽的协会研究在欧洲人识别,并且有 PTC 的一个强壮的协会信号在 rs944289 和 NKX2-1 被观察(在 14q13.3 地点定位了) ,它可能是 PTC 的基因风险因素。这研究试图用汉语与 PTC 检验这基因的协会。有从北中国的汉人口的 PTC 和 360 个健康控制题目的 354 个病人的一个总数在学习被招募。这些个人是 genotyped 决定 rs12589672, rs12894724, rs2076751,和 rs944289。有 PTC 的 rs944289 的协会被获得(C 对 T, P = 0.027,或 = 1.264, 95% CI = 1.026 - 1.557;并且 C/C - C/T 对 T/T, P = 0.034,或 = 1.474, 95% CI = 1.028 - 2.112 ) 。进行亚群分析,我们在 rs944289 的等位基因频率分发发现了边缘的差别(调整 P = 0.062 ) 在有 PTC 和多榴状的甲状腺肿和控制题目的病人之间。我们也观察了一个相互作用(P = 0.029;或 = 2.578, 95% CI = 1.104 - 6.023 ) 在在有 PTC 的病人的 rs944289 和糖尿病之间。在结论, rs944289 在北中国的汉人口与 PTC 的增加的风险被联系,但是没有清楚的协会 NKX2-1 的标签单身者核苷酸多型性在也被观察。 | Lizhe Ai Yaqin Yu Xiaoli Liu Chong Wang Jieping Shi Hui Sun Qiong Yu | 2014 | Frontiers of Medicine2014，8，1： | 0 |
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