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| 1 | Enhancement of cytotoxic T lymphocyte activity by dendritic cells loaded with Tat-protein transduction domain-fused hepatitis B virus core antigen显示文摘人的免疫不全 virus-1-Tat 蛋白质的蛋白质 transduction 领域(PTD ) 有唯一的力量渗透细胞的膜。综合 Tat-PTD 47 的顺序 57 和肝炎 B 病毒核心抗原(HBcAg ) ,我们拼接这些序列并且连接了熔化基因进 pMAL-c2x 向量。熔化蛋白质被亲密关系层析净化并且与骨头搏动了导出髓的树枝状的房间(DC ) ,和 recombinant 蛋白质的 transduction 被免疫检测荧光抗体试金。当 recombinant HBcAg 在房间的表面上被检测时,结果证明 recombinant PTD-HBcAg 能渗透细胞质进 DC。DC 表面分子的百分比 CD86 和主要 histocompatibility 建筑群 II,和 recombinant PTD-HBcAg 导致的 cytokine (IL-12p70 ) 的生产例如 CD80,比 recombinant HBcAg 或肿瘤坏死 factor-α 导致的那些显著地高。与 PTD-HBcAg 对待的 DC 导致了 T 房间区分进特定的细胞毒素的 T 淋巴细胞(CTL ) 并且提高了杀死反应的 CTL。在结论,表示并且净化的 PTD-HBcAg 熔化蛋白质能通过血浆膜渗透进房间,支持 DC 成熟,并且提高 T 房间反应高效地产生 HBcAg 特定的 CTL。 | Xiaohua Chen Yongsheng Yu Qingchun Pan Zhenghao Tang Jinchao Han Guoqing Zang | 2008 | Acta Biochimica et Biophysica Sinica2008，40，12： | 4 |
| 2 | Removal of NO from flue gas by wet scrubbing with NaClO2/( NH2 )2CO solutions显示文摘 | WEI Jinchao LUO Yunbai Yu Ping | 2009 | Journal of Industrial and Engineering Chemistry2009，15，1： | 1 |
| 3 | Absorption of NO in aqueous NaCIO2/Na2CO3 solutions显示文摘 | Wei Jinchao Yu Ping Cai Bo | 2009 | Chemical Engineering and Technology2009，32，1： | 1 |
| 4 | An algebraic multigrid method for finite element systems on criss-cross grids显示文摘 | Shu Shi Xu Jinchao Yang Ying and Yu Haiyuan | 2006 | Advances in Computational Mathematics2006，25，： | 1 |
| 5 | Machine learning powered ellipsometry显示文摘Ellipsometry is a powerful method for determining both the optical constants and thickness of thin films.For decades,solutions to ill-posed inverse ellipsometric problems require substantial human-expert intervention and have become essentially human-in-the-loop trial-and-error processes that are not only tedious and time-consuming but also limit the applicability of ellipsometry.Here,we demonstrate a machine learning based approach for solving ellipsometric problems in an unambiguous and fully automatic manner while showing superior performance.The proposed approach is experimentally validated by using a broad range of films covering categories of metals,semiconductors,and dielectrics.This method is compatible with existing ellipsometers and paves the way for realizing the automatic,rapid,high-throughput optical characterization of films. | Jinchao Liu Di Zhang Dianqiang Yu Mengxin Ren Jingjun Xu | 2021 | Light(Science & Applications)2021，10，4： | 1 |
| 6 | Design and fabrication of r-hirudin loaded dissolving microneedle patch for minimally invasive and long-term treatment of thromboembolic disease显示文摘Cardiovascular disease is the leading cause of global mortality,with anticoagulant therapy being the main prevention and treatment strategy.Recombinant hirudin(r-hirudin)is a direct thrombin inhibitor that can potentially prevent thrombosis via subcutaneous(SC)and intravenous(IV)administration,but there is a risk of haemorrhage via SC and IV.Thus,microneedle(MN)provides painless and sanitary alternatives to syringes and oral administration.However,the current technological process for the micro mould is complicated and expensive.The micro mould obtained via three-dimensional(3D)printing is expected to save time and cost,as well as provide a diverse range of MNs.Therefore,we explored a method for MNs array model production based on 3D printing and translate it to micro mould that can be used for fabrication of dissolving MNs patch.The results show that r-hirudin-loaded and hyaluronic acid(HA)-based MNs can achieve transdermal drug delivery and exhibit significant potential in the prevention of thromboembolic disease without bleeding in animal models.These results indicate that based on 3D printing technology,MNs combined with r-hirudin are expected to achieve diverse customizableMNs and thus realize personalized transdermal anticoagulant delivery for minimally invasive and long-term treatment of thrombotic disease. | Mengfang Wu Tian Xia Yaran Li Tianfa Wang Shijia Yang Jinchao Yu Qiaoyan Liang Teng Shen Min Yu Bing Zhao | 2022 | Asian Journal of Pharmaceutical Sciences2022，17，2： | 1 |
| 7 | Key Issues for AR-Based Digital Reconstruction of Yuanmingyuan Garden显示文摘 | Wang Yongtian Yue Lin Yu Li Jinchao Lei | | 0，，： | 1 |
| 8 | Path planning of mobile robot based on improved potential field显示文摘 | Guo Jinchao Gao Yu Cui Guangzhao | 2013 | Information Technology Journal2013，12，11： | 1 |
| 9 | A compact filter with sharp-transition and wideband-rejection using the novel defected ground structure显示文摘 | YU Weihua MOU Jinchao LI Xiang | 2009 | Journal of Electromagnetic Waves and Applications2009，23，： | 1 |
| 10 | Design of a Compact Ka-band Balanced Mixer Based on a Novel Wide-band Equivalent Circuit of the Schottky Diode显示文摘 | MOU Jinchao YU Weihua Yuan Yong | | 0，，04： | 1 |
| 11 | Absorption of NO in aqueous NaClO2/Na2CO3 solutions 显示文摘 | Wei Jinchao Yu Ping Cai Bo | 2009 | Chemical Engineering and Technology2009，32，1： | 1 |
| 12 | Removal of NO from flue gas by wet scrubbing with NaClO2/ ( NH2 ) 2CO solutions 显示文摘 | Wei Jinchao Luo Yunbai Yu Ping | 2009 | Journal of Industrial and Engineering Chemistry2009，15，1： | 1 |
| 13 | The effect of drug loading and multiple administration on the protein corona formation and brain delivery property of PEG-PLA nanoparticles显示文摘The presence of protein corona on the surface of nanoparticles modulates their physiological interactions such as cellular association and targeting property.It has been shown that α-mangostin(αM)-loaded poly(ethylene glycol)-poly(l-lactide)(PEG-PLA)nanoparticles(NP-αM)specifically increased low density lipoprotein receptor(LDLR)expression in microglia and improved clearance of amyloid beta(Aβ)after multiple administration.However,how do the nanoparticles cross the blood‒brain barrier and access microglia remain unknown.Here,we studied the brain delivery property of PEG-PLA nanoparticles under different conditions,finding that the nanoparticles exhibited higher brain transport efficiency and microglia uptake efficiency afterαM loading and multiple administration.To reveal the mechanism,we performed proteomic analysis to characterize the composition of protein corona formed under various conditions,finding that both drug loading and multiple dosing affect the composition of protein corona and subsequently influence the cellular uptake of nanoparticles in b.End3 and BV-2 cells.Complement proteins,immunoglobulins,RAB5A and CD36 were found to be enriched in the corona and associated with the process of nanoparticles uptake.Collectively,we bring a mechanistic understanding about the modulator role of protein corona on targeted drug delivery,and provide theoretical basis for engineering brain or microglia-specific targeted delivery system. | Yuyun Tang Jinchao Gao Tao Wang Qian Zhang Antian Wang Meng Huang Renhe Yu Hongzhuan Chen Xiaoling Gao | 2022 | Acta Pharmaceutica Sinica B2022，12，4： | 0 |
| 14 | Bacteria-based nanosystems for enhanced antitumor therapy显示文摘As early as the 19th century,people began attempting to treat cancer with bacteria,inspired by the phenomenon wherein tumor growth was slowed or even eradicated after severe bacterial infections in some patients with cancer(Nauts et al.,1946).The earliest documented case is from the German doctor Busch who applied Streptococcus pyogenes to pa-tients with malignant sarcoma and saw significantly reduced tumor volume after treatment(Pawelek et al.,2003).Fol-lowing this,German doctor Fehleisen and American doctor Coley each reported cases wherein patients with malignant sarcoma were cured with Streptococcus pyogenes infection(Fehleisen,1883;Coley,1891).To further improve the safety of bacteria-based cancer treatment,Coley inactivated the bacteria and made bacterial preparations,after which he confirmed that these bacterial preparations still retained an-titumor activity(Coley,1910;Balkwill,2009). | Yu Han Miao Fan Dandan Han Kun Ge Jin Chang Jinchao Zhang | 2022 | Science China(Life Sciences)2022，65，2： | 0 |
| 15 | A highly selective and sensitive upconversion nanoprobe for monitoring hydroxyl radicals in living cells and the liver显示文摘Excessive reactive oxygen species(ROS)would attack living cells and cause a series of oxidative stress related diseases,such as liver damage.Hydroxyl radicals(·OH)are currently known as one of the most toxic and harmful free radicals to organisms.Therefore,studies involving hydroxyl radicals have become important research topics in the fields of biology,biochemistry,and biomedicine.In addition,imaging of analytes using upconversion nanoparticles(UCNPs)possesses significant advantages over that using general fluorescent dyes or nanoparticles due to its high spatial resolution,reduced photodamage,and deep tissue penetration properties.Herein,we designed a highly selective and sensitive hydroxyl radical nanoprobe based on the luminescence resonance energy transfer between upconversion nanoparticles and methylene blue(MB).The concentration of·OH could be determined by the fluorescence recovery of the UCNPs due to the oxidative damage of MB.Using this nanoprobe,the·OH in living cells or in liver tissues could be monitored with high sensitivity and selectivity. | Guangshun Yu Na Feng Dan Zhao Hao Wang Yi Jin Dandan Liu Zhenhua Li Xinjian Yang Kun Ge Jinchao Zhang | 2021 | Science China(Life Sciences)2021，64，3： | 0 |
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