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| 1 | Cold-chain food contamination as the possible origin of COVID-19 resurgence in Beijing显示文摘COVID-19,caused by SARS-CoV-2[1,2],has been contained in China through stringent non-pharmaceutical interventions.Border control and quarantine have effectively prevented the virus from being spread by infected travellers,but the risk of resurgence caused by other routes of introduction and transmission remains unclear,and current strategies to prevent resurgence could be flawed.Since July,SARS-CoV-2 RNA contaminations in frozen food imported from countries with ongoing epidemics have been reported in nine provinces in China[3,4].However,there is no robust evidence of COVID-19 outbreaks initiated by environmentto-human transmission.Here we add to evidence of such transmission by investigating the recent COVID-19 resurgence in Beijing. | Xinghuo Pang Lili Ren Shuangsheng Wu Wentai Ma Jian Yang Lin Di Jie Li Yan Xiao Lu Kang Shichang Du Jing Du Jing Wang Gang Li Shuguang Zhai Lijuan Chen Wenxiong Zhou Shengjie Lai Lei Gao Yang Pan Quanyi Wang Mingkun Li Jianbin Wang Yanyi Huang Jianwei Wang COVID-19 Field Response Group COVID-19 Laboratory Testing Group | 2020 | National Science Review2020，7，12： | 51 |
| 2 | Relationship between hepatitis B virus DNA levels and liver histology in patients with chronic hepatitis B显示文摘瞄准:与长期的肝炎 B (CHB ) 在病人学习在肝炎 B (HBV ) DNA 层次和肝组织学之间的关系并且决定肝炎 B e 的流行和特征抗原(HBeAg )negative 病人。方法:有 CHB 的 213 个病人的一个总数被学习,并且浆液 HBV DNA 层次被 COBAS Amplicor HBV 监视器测量测试。所有病人根据 HBeAg 地位被划分成二个组。在浆液 HBV DNA 层次和肝损坏(肝组织学和生物化学) 之间的关联被探索。结果:有比 105 copies/mL 高的浆液 HBV DNA 层次的 213 个病人,(83.6%) 178 是 HBeAg 积极,(16.4%) 35 是 HBeAg 否定。浆液 HBV DNA 层次没在否定的 HBeAg 或 HBeAg 被相关到肝疾病的年龄, CHB 的历史,组织学的等级和阶段积极病人。在 HBV DNA 和丙氨酸 aminotransferanse (中高音) 的浆液层次之间没有关联,在 HBeAg 积极病人的 aspartate aminotrans-ferase (著名计算机生产厂商) 。在 HBeAg 否定病人,当浆液 DNA 层次与中高音相关时,在 HBV DNA 和著名计算机生产厂商的浆液层次之间没有关联(r = 0.351, P = 0.042 ) 。肝疾病的等级(G) 与中高音和著名计算机生产厂商相关(P <
0.05, r = 0.205, 0.327 分别地) 在 HBeAg 积极病人。在 HBeAg 否定病人,关联在中高音,著名计算机生产厂商和 G 之间被显示出(P <
0.01,并且 r = 0.862, 0.802 分别地) 。 HBeAg 否定病人更老( 35 +/- 9 年对 30 +/- 9 年, P <
0.05 )并且有 HBV 感染的更长的历史( 8 +/- 4 年对 6 +/- 4 年, P <
0.05 )并且更低的 HBV DNA 比 HBeAg 积极病人铺平( 8.4 +/- 1.7 木头 HBV DNA 对 9.8 +/- 1.3 木头 HBV DNA , P <
0.001 )。在在二个组之间的两性比率,中高音和著名计算机生产厂商层次和肝组织学没有有效差量。结论:浆液 HBV DNA 水平没与 HBV DNA 在 CHB 病人被相关到肝疾病的组织学的等级或舞台超过 105 copies/mL。比作 HBeAg 积极病人, HBeAg 否定病人更老并且有更低的 HBV DNA 水平和更长的 HBV 感染历史。在在二个组之间的两性比率,中高音和著名计算机生产厂商层次和肝组织学没有有效差量。 | Jie Shao Lai Wei Hao Wang Yan Sun Lan-Fang Zhang Jing Li Jian-Qiang Dong | 2007 | World Journal of Gastroenterology2007，13，14： | 35 |
| 3 | Network Pharmacology Evaluation Method Guidance-Draft显示文摘Foreword Note:Some of the document contents may involve certain patents,the identification of which is not the responsibility of the institution that releases the document.Main drafting organizations:Tsinghua University,Specialty Committee of Network Pharmacology of World Federation of Chinese Medicine Societies(WFCMS). | Shao Li Yi-Tao Chen Qing-Yang Ding Jian-Ye Dai Xian-Chun Duan Yuan-Jia Hu Xin-Xing Lai Qing-Fei Liu Ming Niu Rong-Wu Xiang Zheng Xiang Hai-Yu Xu Xin Wang Hong-Jun Yang Kuo Yang Ming Yang Hai-Yang Yu Bo Zhang Peng Zhang Qian-Ru Zhang Hui-Hui Zhao Jing Zhao | 2021 | World Journal of Traditional Chinese Medicine2021，7，1： | 32 |
| 4 | HYDRAULICS OF COMPOUND CHANNEL WITH VEGETATED FLOODPLAINS显示文摘Experimental researches were conducted on flood carrying capacity of compound open channels with wide and narrow floodplains as well as hydrodynamic behavior of overbank flow across vegetated floodplains. Results show that hydrodynamic behavior of compound channels with narrow floodplains is different from that of the wide floodplains. In such compound channels with narrow vegetated floodplains as in the Pearl River delta nework, it has been found that vegetated domains become really impenetra ble when the length of vegetated domain is larger than Lm, the length of f low in floodplain coming into the main channel, and that the influence of vegeta ted floodplains upon flood stages of a channel is not very significant. | Huang Ben-sheng, Lai Guan-wen, Qiu Jing, Lin Shu-zhong (Guangdong Research Institute of Water Conservancy & Hydro-Power, Guangzhou 510610, China | 2002 | Journal of Hydrodynamics2002，14，1： | 21 |
| 5 | Allergen micro-array detection of specific IgE-reactivity in Chinese allergy patients显示文摘背景变应原微数组是为浆液 IgE-reactivity.In 屏蔽这学习变应原的强大的工具微数组被用来鉴别在选择中国过敏症 patients.Methods 之中统治IgE有约束力的变应原和跨反应的模式学习从广州的城市用耐心的 sera 被进行,南京,有 Dermatophagoides pteronyssinus ( Der p )的 Chengdu 和 Shenyang.In 总数 100 sera 比 50 kU/L 高的特定的IgE水平为对 103 | ZHENG Yi-wu LI Jing LAI Xu-xin ZHAO De-yu LIU Xiao-fan LIN Xiao-ping Birgitte Gjesing Paola Palazzo Adriano Mari ZHONG Nan-shan Michael D Spangfort | 2011 | Chinese Medical Journal2011，，24： | 16 |
| 6 | Short-term entecavir versus lamivudine therapy for HBeAg-negative patients with acute-on-chronic hepatitis B liver failure显示文摘BACKGROUND: Selection of drugs for antiviral therapy of patients with hepatitis B virus (HBV)-related acute-on-chronic liver failure (ACLF) remains difficult. This study was undertaken to evaluate the short-term efficacy of entecavir versus lamivudine on hepatitis B e antigen (HBeAg)-negative patients with ACLF. METHODS: The data of 182 HBeAg-negative patients with ACLF were retrospectively collected from patient profiles of the hospital. In these patients, 93 HBeAg-negative patients with ACLF were treated orally with 0.5 mg of entecavir and 89 were treated orally with 100 mg of lamivudine every day. The gender and age were matched between the two groups. Biochemical items, the model for end-stage liver disease (MELD) score, and HBV DNA level were matched at baseline between the two groups and monitored during treatment. The 3-month mortalities of the two groups were compared. RESULTS: No significant differences were found in biochemical items, MELD score, and HBV DNA level at baseline (P>0.05). HBV DNA level decreased within 3 months in both groups (P<0.05), regardless of the pretreatment MELD score. In patients with the same range of pretreatment MELD scores, treatment duration, posttreatment HBV DNA levels, percentage of HBV DNA level <2.7 lg copies/mL, biochemical items, MELD scores and 3-month mortality were similar in the two groups (all P>0.05). Pretreatment MELD score was not related to posttreatment HBV DNA levels (P>0.05), but related to a 3-month mortality in both groups (both P<0.001).CONCLUSIONS: In HBeAg-negative patients with ACLF, the short-term efficacy of entecavir versus lamivudine was similar. The degree of pretreatment liver failure significantly affected the outcome of treatment. | Jing Lai Ying Yan Li Mai Yu-Bao Zheng Wei-Qiang Gan Wei-Min Ke | 2013 | Hepatobiliary & Pancreatic Diseases International2013，12，2： | 12 |
| 7 | Piglets cloned from induced pluripotent stem cells显示文摘 | Nana Fan Jijun Chen Zhouchun Shang Hongwei Dou Guangzhen Ji Qingjian Zou Lu Wu Lixiazi He Fang Wang Kai Liu Na Liu Jianyong Han Qi Zhou Dengke Pan Dongshan Yang Bentian Zhao Zhen Ouyang Zhaoming Liu Yu Zhao Lin Lin Chongming Zhong Quanlei Wang Shouqi Wang Ying Xu Jing Luan Yu Liang Zhenzhen Yang Jing Li Chunxia Lu Gabor Vajta Ziyi Li Hongsheng Ouyang Huayan Wang Yong Wang Yang Yang Zhonghua Liu Hong Wei Zhidong Luan Miguel A Esteban Hongkui Deng Huanming Yang Duanqing Pei Ning Li Gang Pei Lin Liu Yutao Du Lei Xiao Liangxue Lai | 2013 | Cell Research2013，23，1： | 12 |
| 8 | Early filiform needle acupuncture for poststroke depression: a meta-analysis of 17 randomized controlled clinical trials显示文摘OBJECTIVE: To evaluate the effectiveness and safety of filiform needle acupuncture for poststroke depression, and to compare acupuncture with the therapeutic efficacy of antidepressant drugs. DATA RETRIEVAL: We retrieved data from the Chinese National Knowledge Infrastructure(1979–2012), Wanfang(1980–2012), VIP(1989–2012), Chinese Biomedical Literature(1975–2012), PubMed(1966–2012), Ovid Lww(–2012), and Cochrane Library(–2012) Database using the internet. SELECTION CRITERIA: Randomized controlled trials on filiform needle acupuncture versus antidepressant drugs for treatment of poststroke depression were included. Moreover, the included articles scored at least 4 points on the Jadad scale. Exclusion criteria: other acupuncture therapies as treatment group, not stroke-induced depression patients, score < 4 points, non-randomized controlled trials, or animal trials. MAIN OUTCOME MEASURES: These were the Hamilton Depression Scale scores, clinical effective rate, Self-Rating Depression Scale scores, Side Effect Rating Scale scores, and incidence of adverse reaction and events. RESULTS: A total of 17 randomized controlled clinical trials were included. Meta-analysis results displayed that after 4 weeks of treatment, clinical effective rate was better in patients treated with filiform needle acupuncture than those treated with simple antidepressant drugs [relative risk = 1.11, 95% confidence interval(CI): 1.03–1.21, P = 0.01]. At 6 weeks, clinical effective rate was similar between filiform needle acupuncture and antidepressant drug groups. At 2 weeks after filiform needle acupuncture, Hamilton Depression Scale(17 items) scores were lower than in the antidepressant drug group(mean difference =-2.34, 95%CI:-3.46 to-1.22, P < 0.000,1). At 4 weeks, Hamilton Depression Scale(24 items) scores were similar between filiform needle acupuncture and antidepressant drug groups. Self-Rating Depression Scale scores were lower in filiform needle acupuncture group than in the antidepressant drug group. Side Effect Rating Scale was used in only two articles, and no meta-analysis was conducted. Safety evaluation of the 17 articles showed that gastrointestinal tract reactions such as nausea and vomiting were very common in the antidepressant drug group. Incidence of adverse reaction and events was very low in the filiform needle acupuncture group. CONCLUSION: Early filiform needle acupuncture for poststroke depression can perfectly control depression. Filiform needle acupuncture is safe and reliable. Therapeutic effects of filiform needle acupuncture were better than those of antidepressant drugs. | Jiping Zhang Jing Chen Junqi Chen Xiaohui Li Xueyan Lai Shaoqun Zhang Shengxu Wang | 2014 | Neural Regeneration Research2014，9，7： | 11 |
| 9 | Science Letters:IGFBP7 plays a potential tumor suppressor role against colorectal carcinogenesis with its expression associated with DNA hypomethylation of exon 1显示文摘Insulin-like growth factor binding-protein-7 (IGFBP7) was obtained from our previous colonic adenocarcinoma (CRC) and normal mucosa suppression subtraction hybridization (SSH) cDNA libraries. By RT-PCR and immunohistochemistry, we found that IGFBP7 was overexpressed in CRC tissue compared to normal tissue. However, our in vitro experiments performed in 10 CRC cell lines showed that IGFBP7 expressed only in SW480 and Caco2 cell lines, which implied an underlying reversible regulatory mechanism. Using methylation-specific PCR (MSP) and bisulfite sodium PCR (BSP), we found that its expression was associated with DNA hypomethylation of exon1. This was further supported by the in vitro study which showed restored IGFBP7 expression after demethylation agent 5-aza-2’-deoxycytidine treatment. Correlation analysis between IGFBP7 expression and prognosis indicated that overexpression of IGFBP7 in CRC tissue correlated with favourable survival. Investigation of the func-tional role of IGFBP7 through transfection studies showed that IGFBP7 protein could inhibit growth rate, decrease colony for-mation activity, and induce apoptosis in RKO and SW620 cells, suggesting it a potential tumor suppressor protein in colorectal carcinogenesis. In conclusion, our study clearly demonstrated that IGFBP7 plays a potential tumor suppressor role against colo-rectal carcinogenesis and its expression is associated with DNA hypomethylation of exon 1. | RUAN Wen-jing LIN Jie XU En-ping XU Fang-ying MA Yu DENG Hong HUANG Qiong LV Bing-jian HU Hu CUI Jing DI Mei-juan DONG Jian-kang LAI Mao-de | 2006 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2006，7，11： | 11 |
| 10 | Higher PD-1 expression concurrent with exhausted CD8+ T cells in patients with de novo acute myeloid leukemia显示文摘Objective: To investigate the association between the T cell inhibitory receptor programmed death 1(PD-1)and T cell exhaustion status in T cells from patients with de novo acute myeloid leukemia(AML) and AML in complete remission(CR).Methods:Surface expression of PD-1 and the exhaustion and immunosenescence markers CD244 and CD57 on CD3+,CD4+ and CD8+ T cells from peripheral blood samples from 20 newly diagnosed,untreated AML patients and 10 cases with AML in CR was analyzed by flow cytometry.Twenty-three healthy individuals served as control.Results:A significantly higher percentage of PD-1+ cells were found for CD3+ T cells in the de novo AML group compared with healthy controls.In addition,an increased level of PD-1+ CD8+ T cells,but not PD-1+ CD4+,was found for CD3+ T cells in the de novo AML and AML-CR samples.A higher percentage of CD244+ CD4+,CD244+ CD8+,CD57+ CD4+ and CD57+ CD8+ T cells was found in CD3+ T cells in samples from those with de novo AML compared with those from healthy controls.Strong increased PD-1+ CD244+ and PD-1+ CD57+ coexpression was found for CD4+ and CD8+ T cells in the de novo AML group compared with healthy controls.Conclusions:We characterized the major T cell defects,including co-expression of PD-1 and CD244,CD57-exhausted T cells in patients with de novo AML,and found a particular influence on CD8+ T cells,suggesting a poor anti-leukemia immune response in these patients. | Jiaxiong Tan Shaohua Chen Yuhong Lu Danlin Yao Ling Xu Yikai Zhang Lijian Yang Jie Chen Jing Lai Zhi Yu Kanger Zhu Yangqiu Li | 2017 | Chinese Journal of Cancer Research2017，29，5： | 10 |
| 11 | Thermodynamic conditions of framework grain dissolution of clastic rocks and its application in Kela 2 gas field显示文摘Feldspar and clastic debris are the most important constituent framework grains of sedimentary clastic rocks and their chemical dissolution plays an essential role in the formation and evolution of the secondary pore in the reservoir rocks. On the basis of thermodynamic phase equilibrium, this study investigates the chemical equilibrium relationships between fluid and various plagioclase and K-feldspar in diagenesis of the sediments, particularly, the impact of temperature and fluid compositions (pH, activity of K+, Na+, Ca2+ and so on) on precipitation and dissolution equilibria of feldspars. Feldspar is extremely easily dissolved in the acid pore water with a low salinity when temperature decreases. The dissolution of anorthite end-member of plagioclase is related to the Ca content of the mineral and the fluid, higher Ca either in the mineral or in the fluid, easier dissolution of the feldspar. Moreover, the dissolution of albite end-member of plagioclase is related to Na of both the mineral and fluid, lower Na out of both the mineral and fluid, easier dissolution of the mineral. Similarly, lower-K fluid is more powerful to dissolve K-feldspar than the higher. The anorthite component of plagioclase is most easily dissolved in ground water-rock system, the albite is the secondary, and K-feldspar is the most stable. Selective dissolution of plagioclase occurs in diagenesis because of the plagioclase solid solution that is mainly composed of albite and anorthite end-members, Ca-rich part of which is preferentially dissolved by the pore water, in contrast to the Na-rich parts. Based on investigation of reservoir quality, secondary pore, dissolution structures of feldspar, clay minerals and ground water chemistry of the Kela 2 gas field of Kuqa Depression in the Tarim basin of Western China, we discovered that the secondary pore is very well developed in the highest quality section of the reservoir, and the plagioclase of which was obviously selectively dissolved, in contrast to the overgrowth of K-feldspar. Chemistry of the ground water out of the highest quality section of the reservoir is consistent with the complete dissolution field of anorthite, partial dissolution field of albite component of plagioclase, and the precipitation of K-feldspar on the temperature-logK phase equilibrium diagram drawn from this study. The thermodynamic modeling calculation gave us an insight into formation of the secondary pore within the reservoir, and the dissolution of plagioclase an important cause of the secondary pore. It is assumed that the solvent to dissolve the plagioclase frame grains originated from the origin-enriched compacted fluid acids out of the mudstone buried beneath the top of geopressured zones in the Kuqa Depression. | LAI Xingyun, YU Bingsong, CHEN Junyuan, CHEN Xiaolin, LIU Jianqing, MEI Mingxiang, JING Weiguang & CHENG Suhua School of Earth Sciences and Mineral Resources, China University of Geosciences, Beijing 100083, China Key Laboratory of Lithospheric Tectonics, Deep-level Process and Exploration of Ministry of Education, Beijing 100083, China | 2005 | Science China Earth Sciences2005，48，1： | 9 |
| 12 | Melatonin ameliorates microvessel abnormalities in the cerebral cortex and hippocampus in a rat model of Alzheimer’s disease显示文摘Melatonin can attenuate cardiac microvascular ischemia/reperfusion injury,but it remains unclear whether melatonin can also ameliorate cerebral microvascular abnormalities.Rat models of Alzheimer’s disease were established by six intracerebroventricular injections of amyloidbeta 1–42,administered once every other day.Melatonin(30 mg/kg)was intraperitoneally administered for 13 successive days,with the first dose given 24 hours prior to the first administration of amyloid-beta 1–42.Melatonin ameliorated learning and memory impairments in the Morris water maze test,improved the morphology of microvessels in the cerebral cortex and hippocampus,increased microvessel density,alleviated pathological injuries of cerebral neurons,and decreased the expression of vascular endothelial growth factor and vascular endothelial growth factor receptors 1 and 2.These findings suggest that melatonin can improve microvessel abnormalities in the cerebral cortex and hippocampus by lowering the expression of vascular endothelial growth factor and its receptors,thereby improving the cognitive function of patients with Alzheimer’s disease.This study was approved by the Animal Care and Use Committee of Jinzhou Medical University,China(approval No.2019015)on December 6,2018. | Pan Wang Hai-Juan Sui Xiao-Jia Li Li-Na Bai Jing Bi Hong Lai | 2021 | Neural Regeneration Research2021，16，4： | 8 |
| 13 | Safety evaluation for railway vehicles using an improved indirect measurement method of wheel–rail forces显示文摘The wheel–rail force measurement is of great importance to the condition monitoring and safety evaluation of railway vehicles. In this paper, an improved indirect method for wheel–rail force measurement is proposed to evaluate the running safety of railway vehicles. In this method, the equilibrium equations of a suspended wheelset are derived and the wheel–rail forces are then be obtained from measured suspension and inertia forces. This indirect method avoids structural modifications to the wheelset and is applicable to the long-term operation of railway vehicles.As the wheel–rail lateral forces at two sides of the wheelset are difficult to separate, a new derailment criterion by combined use of wheelset derailment coefficient and wheel unloading ratio is proposed. To illustrate its effectiveness,the indirect method is applied to safety evaluation of railway vehicles in different scenarios, such as the cross wind safety of a high-speed train and the safety of a metro vehicle with hunting motions. Then, the feasibility of using this method to identify wheel–rail forces for low-floor light rail vehicles with resilient wheels is discussed. The values identified by this method is compared with that by Simpack simulation for the same low-floor vehicle, which shows a good coincidence between them in the time domain of the wheelset lateral force and the wheel–rail vertical force. In addition, use of the method to determine the high-frequency wheel–rail interaction forces reveals that it is possible to identify the high-frequency wheel–rail forces through the accelerations on the axle box. | Jing Zeng Lai Wei Pingbo Wu | 2016 | Journal of Modern Transportation2016，24，2： | 6 |
| 14 | Clinical analysis of 23 patients pathologically diagnosed with primary and secondary pulmonary enteric adenocarcinoma显示文摘To the Editor:Primary pulmonary enteric adenocarcinoma (P-PEAC), classified as a variant of lung adenocarcinoma with invasive mucinous adenocarcinoma, colloid adenocarcinoma, and fetal adenocarcinoma by the International Association for the Study of Lung Cancer, American Thoracic Society, and European Respiratory Society, was a rare type of lung adenocarcinoma.In clinical practices, P-PEAC is easily confused with secondary pulmonary enteric adenocarcinoma (S-PEAC) that is manifested as pulmonary infiltration onset. Therefore, a retrospective study was carried out to investigate the difference of clinical manifestation, imaging presentation, immunohistochemical staining, and molecular pathological characteristics of P-PEAC and S-PEAC. | Lei Gu Xu-Zhou Wang Wen Wen Jing Lin Xi-Fang Chen Guo-Xiang Lai Lu Chen Xiao-Juan Ouyang Lei Zhang Jia Ye Yue-Bin Liu De-Hua Zeng De-Ling Liu Wei Liu | 2019 | Chinese Medical Journal2019，，11： | 6 |
| 15 | 15-PGDH is reduced and induces apoptosis and cell cycle arrest in gastric carcinoma显示文摘AIM: To investigate the expression of 15-hydroxyprostaglandin dehydrogenase (15-PGDH) in human gastric cancer and it's mechanism in apoptosis and cell cycle arrest. METHODS: Expression of 15-PGDH mRNA and protein was examined by immunohistochemistry, immunocytochemistry, reverse transcriptase polymerase chain reaction (RT-PCR) and Western blotting in tissue from human gastric cancer, gastric precancerous state (gastric polyps and atrophic gastritis), normal stomach, and gastric cancer cell lines. The relationship between gastric cancer, gastric precancerous state and 15-PGDH expression was determined. The association between expression of 15-PGDH and various clinicopathological parameters in gastric cancer was evaluated. Human gastric cancer cell line SGC-7901 was transfected with 15-PGDH expression plasmids. The effect of 15-PGDH on the cell cycle was examined by flow cytometry. The effect of 15-PGDH on apoptosis was examined by transmission electron microscopy, flow cytometry and transferasemediated nick end labeling (TUNEL) assay. Expression of cell cycle (p21, p27, p16 and p53) and apoptosis (Survivin, BCL-2, BCL-XL, BAK and BAX) genes was analyzed by RT-PCR. RESULTS: Expression of 15-PGDH mRNA and protein in human gastric cancer tissues was significantly lower than in normal gastric tissues (P < 0.01). Expression in human gastric cancer cell lines MKN-28 and MKN-45 was reduced, and absent in SGC-7901 cells (P < 0.05). Reduction of 15-PGDH expression was also found in precancerous tissues, such as gastric polyps and atrophic gastritis (P < 0.01). There was a significant difference in expression of 15-PGDH among various gastric cancer pathological types (P < 0.05), with or without distant metastasis (P < 0.05) and different TNM stage (P < 0.01). Flow cytometry demonstrated a significant increase in apoptotic cells in SGC-7901 cells transfected with pcDNA3/15-PGDH plasmid for 24 and 48 h (P < 0.01), and an increased fraction of sub-G1 phase after transfection (P < 0.05). TUNEL assay showed an increased Apoptotic Index in cells overexpressing 15-PGDH (P < 0.01). After transfection, expression of proapoptotic genes, such as BAK (P < 0.05), BAX and p53 (P < 0.01), was increased. Expression of antiapoptotic genes was decreased, such as Survivin, BCL-2 and BCL-XL (P < 0.01). Expression of cyclin-dependent kinase inhibitors p21 and p16 (P < 0.01) was significantly upregulated in cells overexpressing 15-PGDH. CONCLUSION: Reduction of 15-PGDH is associated with carcinogenesis and development of gastric carcinoma. 15-PGDH induces apoptosis and cell cycle arrest in SGC-7901 cells. | Li-Hong Lou Da-Dao Jing Yue-Xing Lai Ying-Ying Lu Ji-Kun Li Kai Wu | 2012 | World Journal of Gastroenterology2012，18，10： | 6 |
| 16 | Toxic effect of acrylamide on the development of hippocampal neurons of weaning rats显示文摘Although numerous studies have examined the neurotoxicity of acrylamide in adult animals,the effects on neuronal development in the embryonic and lactational periods are largely unknown.Thus,we examined the toxicity of acrylamide on neuronal development in the hippocampus of fetal rats during pregnancy.Sprague-Dawley rats were mated with male rats at a 1:1 ratio.Rats were administered 0,5,10 or 20 mg/kg acrylamide intragastrically from embryonic days 6–21.The gait scores were examined in pregnant rats in each group to analyze maternal toxicity.Eight weaning rats from each group were also euthanized on postnatal day 21 for follow-up studies.Nissl staining was used to observe histological change in the hippocampus.Immunohistochemistry was conducted to observe the condition of neurites,including dendrites and axons.Western blot assay was used to measure the expression levels of the specific nerve axon membrane protein,growth associated protein 43,and the presynaptic vesicle membrane specific protein,synaptophysin.The gait scores of gravid rats significantly increased,suggesting that acrylamide induced maternal motor dysfunction.The number of neurons,as well as expression of growth associated protein 43 and synaptophysin,was reduced with increasing acrylamide dose in postnatal day 21 weaning rats.These data suggest that acrylamide exerts dose-dependent toxic effects on the growth and development of hippocampal neurons of weaning rats. | Sheng-min Lai Zi-ting Gu Meng-meng Zhao Xi-xia Li Yu-xin Ma Li Luo Jing Liu | 2017 | Neural Regeneration Research2017，12，10： | 6 |
| 17 | Defective Kernel 39 encodes a PPR protein required for seed development in maize显示文摘RNA editing is a posttranscriptional process that is important in mitochondria and plastids of higher plants. All RNA editing-specific trans-factors reported so far belong to PLS-class of pentatricopeptide repeat(PPR)proteins. Here, we report the map-based cloning and molecular characterization of a defective kernel mutant dek39 in maize. Loss of Dek39 function leads to delayed embryogenesis and endosperm development, reduced kernel size, and seedling lethality. Dek39 encodes an E subclass PPR protein that targets to both mitochondria and chloroplasts, and is involved in RNA editing in mitochondrial NADH dehydrogenase3(nad3) at nad3-247 and nad3-275. C-to-U editing of nad3-275 is not conserved and even lost in Arabidopsis, consistent with the idea that no close DEK39 homologs are present in Arabidopsis. However, the amino acids generated by editing nad3-247 and nad3-275 are highly conserved in many other plant species, and the reductions of editing at these two sites decrease the activity of mitochondria NADH dehydrogenase complex I,indicating that the alteration of amino acid sequence is necessary for Nad3 function. Our results indicate that Dek39 encodes an E sub-class PPR protein that is involved in RNA editing of multiple sites and is necessary for seed development of maize. | Xiaojie Li Wei Gu Silong Sun Zongliang Chen Jing Chen Weibin Song Haiming Zhao Jinsheng Lai | 2018 | Journal of Integrative Plant Biology2018，60，1： | 6 |
| 18 | Validity assessment and determination of the cutoff value for the Index of Complexity, Outcome and Need among 12-13 year-olds in Southern Chinese显示文摘To validate the use of the Index of Complexity,Outcome and Need (ICON) in assessing orthodontic treatment need among 12-13 year-olds in southern China,we determined the threshold value of ICON based on Chinese orthodontists' judgments. The samples consisted of 335 students in grade 7 from 16 randomly selected middle schools in Chengdu,China. Three associate professors provided ICON scores for each participant and the results were compared with the gold standard judgments from 25 experts on treatment needs. Based on the gold standard,195 casts belonged to the treatment category,while the rest 140 belonged to the no-treatment category. With the international cutoff point of 43,the sensitivity and specificity of the ICON score were 0.29 and 0.98. The best compromise between sensitivity and specificity in Chengdu,compared with the gold standard,was found at a cutoff point of 29,and the sensitivity and specificity were 0.88 and 0.83. When used to evaluate the treatment need of 12-13 year-olds in southern China,the international ICON cutoff value did not correspond well with Chinese orthodontists' judgments; a lower cutoff value of 29 offered a greater sensitivity and specificity with respect to expert orthodontists' perception of treatment need. | Zheng-Yu Liao Fan Jian Hu Long Yun Lu Yan Wang Zhi Yang Yu-Wei He Peter Wamalwa Jing Wang Nian-Song Ye Sheng Wang Wen-Li Lai | 2012 | International Journal of Oral Science2012，4，2： | 5 |
| 19 | Polymyxin for the treatment of intracranial infections of extensively drug-resistant bacteria in children after neurosurgical operation显示文摘Background Increased meningitis caused by extensively drug-resistant bacillary presents a significant challenge in antibiotic selection.The aim of our study was to evaluate the efficacy and safety of polymyxin in the treatment of post-neurosurgical meningitis due to the extensively drug-resistant bacillary in children.Methods We performed a retrospective study on post-neurosurgical meningitis caused by the extensively drug-resistant bacillary in children,who were treated with polymyxin for≥3 days.Results Among five post-neurosurgical meningitis cases that were included,the children were infected by Acinetobacter baumannii(n=3),Klebsiella pneumonia(n=l),and Pseudomonas aeruginosa(n=1).The drug susceptibility test showed that they were extensively drug-resistant bacillary.Two patients received intravenous polymyxin E.Three children received intravenous combined with intraventricular injection of polymyxin B.One patient infected by Klebsiella pneumonia eventually died of septic shock.No serious adverse effects of polymyxin were observed.Conclusions Polymyxin is a safe and effective therapy for post-neurosurgical,multidrug-resistant bacillary meningitis in children. | Jing Ye Lin-Hua Tan Zhi-Peng Shen Yun-Song Yu Deng-Ming Lai Jie Fan Qiang Shu | 2020 | World Journal of Pediatrics2020，16，5： | 5 |
| 20 | The mechanism of side reaction induced capacity fading of Ni-rich cathode materials for lithium ion batteries显示文摘Ni-rich cathode materials show great potential of applying in high-energy lithium ion batteries,but their inferior cycling stability hinders this process.Study on the electrode/electrolyte interfacial reaction is indispensable to understand the capacity failure mechanism of Ni-rich cathode materials and further address this issue.This work demonstrates the domain size effects on interfacial side reactions firstly,and further analyzes the inherent mechanism of side reaction induced capacity decay through comparing the interfacial behaviors before and after MgO coating.It has been determined that LiF deposition caused thicker SEI films may not increase the surface film resistance,while HF erosion induced surface phase transition will increase the charge transfer resistance,and the later plays the dominant factor to declined capacity of Ni-rich cathode materials.This work suggests strategies to suppress the capacity decay of layered cathode materials and provides a guidance for the domain size control to match the various applications under different current rates. | Daozhong Hu Yuefeng Su Lai Chen Ning Li Liying Bao Yun Lu Qiyu Zhang Jing Wang Shi Chen Feng Wu | 2021 | Journal of Energy Chemistry2021，30，7： | 5 |
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