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| 1 | Cardiomyocyte overexpression of miR-27b induces cardiac hypertrophy and dysfunction in mice显示文摘最近的研究开始在心脏的肥大和机能障碍的致病揭示了 microRNAs (miRNAs ) 的关键角色。在这研究,我们测试了是否转变生长 factor-β(TGF-β) 调整 miRNA 在心脏的肥大和心失败(HF ) 的发展起了一个枢轴的作用。我们观察到 miR-27b 是在 cardiomyocyte 特定的 Smad4 猛烈老鼠的心的 upregulated,它开发了心脏的肥大。在 vitro,实验证明 miR-27b 表示能被 TGF-β 禁止; 1 并且它的 overexpression 支持了 hypertrophic 房间生长,当 miR-27b 抑制导致了 hypertrophic 房间的抑制时,生长由 phenylephrine (PE ) 引起了处理。而且,有 miR-27b 的 cardiomyocyte 特定的 overexpression 的转基因的老鼠的分析表明 miR-27b overexpression 是足够的导致心脏的肥大和机能障碍。我们验证了 peroxisome 激活 proliferator 的 receptor-γ(PPAR-γ) 作为在 cardiomyocyte 的 miR-27b 的一个直接目标。一致地, miR-27b 转基因的老鼠显著地显示了 PPAR-γ 的底层;比控制鼠标。而且,在用在一只 pressure-overload-induced 老鼠的特定的 antagomir 的 miR-27b 的 vivo silencing, HF 的模型增加了心脏的 PPAR-γ表示,稀释心脏的肥大和机能障碍。我们的学习的结果表明那 TGF-β 1-regulated miR-27b 涉及心脏的肥大的规定,并且为心脏病作为一个有效治疗学的目标验证 miR-27b。 | Jian Wang Yao Song Yan Zhang Han Xiao Qiang Sun Ning Hou Shuilong Guo Youliang Wang Kaiji Fan Dawei Zhan Lagabaiyila Zha Yang Cao Zhenhua Li Xuan Cheng Youyi Zhang Xiao Yang | 2012 | Cell Research2012，22，3： | 36 |
| 2 | Natural Variation in CCD4 Promoter Underpins Species-Specific Evolution of Red Coloration in Citrus Peel显示文摘Carotenoids and apocarotenoids act as phytohormones and volatile precursors that influence plant development and confer aesthetic and nutritional value critical to consumer preference.Citrus fruits display considerable natural variation in carotenoid and apocarotenoid pigments.In this study,using an integrated genetic approach we revealed that a 5;c/s-regulatory change at CCD4b encoding CAROTENOID CLEAVAGE DIOXYGENASE 4b is a major genetic determinant of natural variation in C3 0 apocarotenoids responsible for red coloration of citrus peel.Functional analyses demonstrated that in addition the known role in synthesizing 3-citraurin,CCD4b is also responsible for the production of another important C3 0 apocarotenoid pigment,p-citraurinene.Furthermore,analyses of the CCD4b promoter and transcripts from various citrus germplasm accessions established a tight correlation between the presence of a putative 5'c/s-regulatory enhancer within an MITE transposon and the enhanced allelic expression of CCD4b in C3 0 apocarotenoid-rich red-peeled accessions.Phylogenetic analysis provided further evidence that functional diversification of CCD4b and naturally occurring variation of the CCD4b promoter resulted in the stepwise evolution of red peels in mandarins and their hybrids.Taken together,our findings provide new insights into the genetic and evolutionary basis of apocarotenoid diversity in plants,and would facilitate breeding efforts that aim to improve the nutritional and aesthetic value of citrus and perhaps other fruit crops. | Xiongjie Zheng Kaijie Zhu Quan Sun Weiyi Zhang Xia Wang Hongbo Cao Meilian Tan Zongzhou Xie Yunliu Zeng Junli Ye Lijun Chai Qiang Xu Zhiyong Pan Shunyuan Xiao Paul D.Fraser Xiuxin Deng | 2019 | Molecular Plant2019，12，9： | 18 |
| 3 | Genome-wide Characterization of cis-acting Elements in the Promoters of Key Carotenoid Pathway Genes from the Main Species of Genus Citrus显示文摘Carotenoids are indispensable for both human health and plant survival.Citrus,is one of the fruit crops richest in carotenoid compounds,with approximately 115 kinds of carotenoids;tremendous diversity in carotenoids composition and concentration exists among various species,showing different colors from nearly white to crimson.The carotenoid biosynthetic pathway and the key carotenogenic genes have been identified in citrus;however,the underlying regulatory mechanisms remain unclear.In this study,among the main species of genus Citrus(primitive,wild,and cultivated),we detected carotenoids in flavedo using High-Performance Liquid Chromatography,and analyzed variations in cis-acting elements in the promoters of key carotenoid pathway genes.Intriguingly,both carotenoid composition and content were generally increased during the evolution of citrus,and the corresponding variations in the promoters were identified,including the gain or loss of critical environmental stress-responsive elements and hormone-responsive elements,which are closely associated with carotenoid enhancement.In addition,pummelo has the most heat-responsive elements,but the Mangshan mandarin does not have this element in the promoters of PSY,which is highly related to their geographical origin and indicate that temperature is a critical environmental signal influencing carotenoid accumulation.Moreover,the abscisic acid-responsive motif was rich in almost all the seven species,but the ethylene-responsive motif was deficient,which demystified the unique phytohormone regulation mechanism of carotenoid accumulation in citrus.Overall,our study provides new insights into the molecular regulatory mechanism of carotenoid enhancement in the evolution of citrus,which can facilitate breeding and cultivation efforts to improve the nutritional quality and esthetic value in citrus and hopefully other fruit crops. | Kaijie Zhu Qingjiang Wu Yue Huang Junli Ye Qiang Xu Xiuxin Deng | 2020 | Horticultural Plant Journal2020，6，6： | 8 |
| 4 | Online condition diagnosis for a two-stage gearbox machinery of an aerospace utilization system using an ensemble multi-fault features indexing approach显示文摘China manned space station is designed to operate for over ten years. Long-term and sustainable research on space science and technology will be conducted during its operation. The application payloads must meet the ‘‘long life and high reliability' mission requirement. Gearbox machinery is one of the essential devices in an aerospace utilization system, failure of which may lead to downtime loss even during some disastrous catastrophes. A fault diagnosis of gearbox has attracted attentions for its significance in preventing catastrophic accidents and guaranteeing sufficient maintenance. A novel fault diagnosis method based on the Ensemble Multi-Fault Features Indexing(EMFFI) approach is proposed for the condition monitoring of gearboxes. Different from traditional methods of signal analysis in the one-dimensional space, this study employs a supervised learning method to determine the faults of a gearbox in a two-dimensional space using the classification model established by training the features extracted automatically from diagnostic vibration signals captured. The proposed method mainly includes the following steps. First, the vibration signals are transformed into a bi-spectrum contour map utilizing bi-spectrum technology,which provides a basis for the following image-based feature extraction. Then, Speeded-Up Robustness Feature(SURF) is applied to automatically extract the image feature points of the bi-spectrum contour map using a multi-fault features indexing theory, and the feature dimension is reduced by Linear Discriminant Analysis(LDA). Finally, Random Forest(RF) is introduced to identify the fault types of the gearbox. The test results verify that the proposed method based on the multi-fault features indexing approach achieves the target of high diagnostic accuracy and can serve as a highly effective technique to discover faults in a gearbox machinery such as a two-stage one. | Min ZHOU Ke WANG Yang WANG Kaiji LUO Hongyong FU Liang SI | 2019 | Chinese Journal of Aeronautics2019，32，5： | 6 |
| 5 | CNV analysis of Meishan pig by nextgeneration sequencing and effects of AHR gene CNV on pig reproductive traits显示文摘Background:Reproductive performance of livestock is an economically important aspect of global food production.The Chinese Meishan pig is a prolific breed,with an average of three to five more piglets per litter than European breeds;however,the genetic basis for this difference is not well understood.Results:In this study,we investigated copy number variations(CNVs)of 32 Meishan pigs and 29 Duroc pigs by nextgeneration sequencing.A genome-wide analysis of 61 pigs revealed 12,668 copy number variable regions(CNVRs)that were further divided into three categories based on copy number(CN)of the whole population,i.e.,gain(n=7,638),and loss(n=5,030)CNVRs.We then compared Meishan and Duroc pigs and identified 17.17Mb of 6,387 CNVRs that only existing in Meishan pigs CNVRs that overlapped the reproduction-related gene encoding the aryl hydrocarbon receptor(AHR)gene.We found that normal AHR CN was more frequent than CN loss in four different pig breeds.An association analysis showed that AHR CN had a positive effect on litter size(P<0.05)and that a higher CN was associated with higher total number born(P<0.05),number born alive(P<0.05),number of weaned piglets,and birth weight.Conclusions:The present study provides comprehensive CNVRs for Meishan and Duroc pigs through large-scale population resequencing.Our results provide a supplement for the high-resolution map of copy number variation in the porcine genome and valuable information for the investigation of genomic structural variation underlying traits of interest in pig.In addition,the association results provide evidence for AHR as a candidate gene associated with reproductive traits that can be used as a genetic marker in pig breeding programs. | Xianrui Zheng Pengju Zhao Kaijie Yang Chao Ning Haifei Wang Lei Zhou Jianfeng Liu | 2020 | Journal of Animal Science and Biotechnology2020，11，3： | 6 |
| 6 | In uence of Particle Size on Laser Absorption and Scanning Track Formation Mechanisms of Pure Tungsten Powder During Selective Laser Melting显示文摘A three-dimensional laser absorption model based on ray tracing was established to describe the coupled interaction of a laser beam with particles in the powder layers of pure tungsten(W)material processed by selective laser melting(SLM).The influence of particle size on the powder-to-laser absorptivity and underlying absorption behavior was investigated.An intrinsic relationship between the absorption,distribution of absorbed irradiance within the powder layers,and surface morphology and geometric characteristics(e.g.,contact angle,width and height of tracks,and remelted depth)of the laser scanning tracks is presented here.Simulation conclusions indicate that the absorptivity of the powder layers considerably exceeds the single powder particle value or the dense solid material value.With an increase in particle size,the powder layer absorbs less laser energy.The maximum absorptivity of theWpowder layers reached 0.6030 at the particle size of 5 lm.The distribution of laser irradiance on the particle surface was sensitive to particle size,azimuthal angle,and the position of the powder particles on the substrate.The maximum irradiance in the powder layers decreased from 1.117×10^–3 to 0.85×10^–3W·μm^-2 and the contour of the irradiance distribution in the center of the irradiated area gradually contracted when the particle size increased from 5 to 45 lm.An experimental study on the surface morphologies and cross-sectional geometric characteristics of SLM-fabricated W material was performed,and the experimental results validated the mechanisms of the powder-to-laser-absorption behavior that were obtained in simulations.This work provides a scientific basis for the application of the ray-tracing model to predict the wetting and spreading ability of melted tracks during SLM additive manufacturing in order to yield a sound laser processability. | Jiayao Zhang Dongdong Gu Ying Yang Hongmei Zhang Hongyu Chen Donghuai Dai Kaijie Lin | 2019 | Engineering2019，5，4： | 6 |
| 7 | 参麦注射液防治晚期非小细胞肺癌化疗不良反应的临床观察(英文)显示文摘Objective: To observe the efficacy of Shenmai injection in the treatment for adverse reactions of chemotherapy on advanced non-small cell lung cancer (NSCLC). Methods: 45 NSCLC patients with stages IIIb-IV were randomly divided into two groups: the treatment group (treated by chemotherapy combined with Shenmai injection) and the control group (treated by chemotherapy only). The efficacy of the two groups was evaluated after 3 cycles of treatment. Results: There was no significant difference between the two groups in the recent curative effects (P > 0.05), while there were significant differences between them in Karnofsky score and weight (P < 0.05). The treatment group was better than the control group in preventing leucopenia and decreased hemoglobin, and significant differences were found between them (P < 0.05). The incidence of thrombocytopenia, nausea and vomiting, hepatic and renal dysfunction in the treatment group was lower than that in the control group, but no significant differences were found between them (P > 0.05). Conclusion: Shenmai injection would not influence the efficacy of chemotherapy on advanced NSCLC patients, while it could improve the quality of life, increase the body weight of patients, alleviate adverse reactions of chemotherapy as myelosuppression so as to improve the tolerance of organism to chemotherapy. | Yang Cao Yan Li Yuzhen Bi Peng Li Ruishen Chen Yusheng Wu Kaiji Tan | 2008 | The Chinese-German Journal of Clinical Oncology2008，7，2： | 6 |
| 8 | Recent Progress of Atmospheric Water Harvesting Using Metal-Organic Frameworks显示文摘Atmospheric water harvesting based on vapor adsorption is a newly emerged and potential technology to supply portable water for arid areas.To efficiently harvest vapor from the air,sorbents are required to have conside-rable adsorption capacity,easy regeneration and high stability.With the advantages of porous structure,tunable pore size and tailorable hydrophilicity,metal-organic frameworks(MOFs)have demonstrated excellent performance in vapor adsorption and water generation.In this review,we first discuss the degradation mechanisms of MOFs exposed to water and summarize the structure-stability relationship;by centering on the adsorption isotherms,the connection between the structure of MOFs and the water adsorption property is illuminated;finally,some prospects are suggested in order to push forward the progress of this technology. | PAN Tingting YANG Kaijie HAN Yu | 2020 | Chemical Research in Chinese Universities2020，36，1： | 4 |
| 9 | Unbiased subgenome evolution following a recent whole-genome duplication in pear(Pyrus bretschneideri Rehd.)显示文摘Genome fractionation(also known as diploidization)frequently occurs following paleopolyploidization events.Biased fractionation between subgenomes has been found in some paleo-allopolyploids,while this phenomenon is absent in paleo-autopolyploids.Pear(Pyrus bretschneideri Rehd.)experienced a recent whole-genome duplication(WGD,~30 million years ago);however,the evolutionary fate of the two subgenomes derived from this WGD event is not clear.In this study,we identified the two paleo-subgenomes in pear using peach(Prunus persica)as an outgroup and investigated differences in the gene loss rate,evolutionary rate,gene expression level,and DNA methylation level between these two subgenomes.Fractionation bias was not found between the two pear subgenomes,which evolved at similar evolutionary rates.The DNA methylation level of the two subgenomes showed little bias,and we found no expression dominance between the subgenomes.However,we found that singleton genes and homeologous genes within each subgenome showed divergent evolutionary patterns of selective constraints,expression and epigenetic modification.These results provide insights into subgenome evolution following paleopolyploidization in pear. | Qionghou Li Xin Qiao Hao Yin Yuhang Zhou Huizhen Dong Kaijie Qi Leiting Li Shaoling Zhang | 2019 | Horticulture Research2019，6，1： | 4 |
| 10 | The Variation of Stone Cell Content in 236 Germplasms of Sand Pear(Pyrus pyrifolia)and Identification of Related Candidate Genes显示文摘Stone cells have been described to substantially influence pear fruit quality,as lignin and cellulose are the main components of stone cells.However,there are limited studies on the relationship between the variation and molecular basis of stone cells,lignin and cellulose content among different pear varieties.Here,to reveal the variation of stone cell content within different cultivated species,we collected 236 germplasms of sand pear(Pyrus pyrifolia)at 50 days after flower blooming(DAFB),the key stage of stone cell formation.In our results,we measured the content of stone cells,lignin and cellulose and found that these contents ranged from2.82%to 29.00%,8.84%to 55.30%and 11.52%to 30.55%,respectively.Further analysis showed that the variation coefficient of stone cell,lignin and cellulose content was 39.10%,28.03%and 16.71%,respectively.Additionally,a significant correlation between stone cell,lignin and cellulose content were detected,and the correlation coefficient between the contents of stone cell and lignin(0.912)was higher than between the contents of stone cell and cellulose(0.796).Moreover,the average lignin content(29.73%)was higher than the average cellulose content(18.03%)in stone cells in pear fruits,indicating that lignin is the main component of stone cell in pears.Finally,on the basic of the transcriptome data,we identified 10 transcription factors belonging to bHLH,ERF,MYB,and NAC transcript families,which might be involved in lignin formation in stone cells.qRT-PCR experiments verified coincident trends between expression of candidate genes and stone cell content.This research laid foundation for future studies on genetic variation of stone cells in pear fruits and provided important gene resources for stone cell regulation. | Jiaying Zhang Jiaming Li Cheng Xue Runze Wang Mingyue Zhang Kaijie Qi Jing Fan Hongju Hu Shaoling Zhang Jun Wu | 2021 | Horticultural Plant Journal2021，7，2： | 3 |
| 11 | Amplification of terahertz/infrared field at the nodes of Ranvier for myelinated nerve显示文摘The myelination of axons was the last major evolution in the vertebrate nervous system.Myelin promotes the speed of action potential by two orders,and modulates the conduction of neurons,important for learning new skills.However,the intrinsic mechanism for high-speed information propagation in myelin in the nervous systems is still unclear.We propose that myelinated nerve fibres serve as dielectric waveguides for the high-frequency electromagnetic information in a certain mid-infrared to terahertz spectral range.Based on the structure characteristics of myelinated nerve composed of periodic nodes of Ranvier and myelin sheath,the energy for the signal propagation is supplied and amplified when crossing the nodes of Ranvier via a periodic relay.In this work,we exploit the quasi-quantum model of amplification for neural terahertz/infrared information at the nodes of Ranvier,and prove the existence of biomolecular ensemble for three-energy-level amplification,revealing the essential mechanism of high-speed electromagnetic information transmitting in myelinated nerves. | YanSheng Liu KaiJie Wu ChunLiang Liu GangQiang Cui Chao Chang GuoZhi Liu | 2020 | Science China(Physics,Mechanics & Astronomy)2020，63，7： | 3 |
| 12 | PbMC1a/1b regulates lignification during stone cell development in pear(Pyrus bretschneideri)fruit显示文摘Programmed cell death(PCD)and secondary cell wall(SCW)thickening in pear fruit are accompanied by the deposition of cellulose and lignin to form stone cells.Metacaspase is an important protease for development,tissue renewal and PCD.The understanding of the molecular mechanism whereby pear(Pyrus)metacaspase promotes PCD and cell wall lignification is still limited.In this study,the Metacaspases gene family(PbMCs)from P.bretschneideri was identified.PbMC1a/1b was associated with lignin deposition and stone cell formation by physiological data,semiquantitative real-time polymerase chain reaction(RT-PCR)and quantitative RT-PCR(qRT-PCR).Relative to wildtype(WT)Arabidopsis,the overexpression of PbMC1a/1b increased lignin deposition and delayed growth,thickened the cell walls of vessels,xylary fibers and interfascicular fibers,and increased the expression of lignin biosynthetic genes.Yeast two-hybrid(Y2H),bimolecular fluorescence complementation(BiFC)and GST pull-down assays indicated that the PbMC1a/1b protein physically interacted with PbRD21.Simultaneously,the transient expression of PbMC1a/1b and PbRD21 led to significant changes in the expression of genes and lignin contents in pear fruits and flesh calli.These results indicate that PbMC1a/1b plays an important role in cell wall lignification,possibly by interacting with PbRD21 to increase the mRNA levels of some lignin synthesis-associated genes and promote the formation of stone cells in pear fruit. | Xin Gong Zhihua Xie Kaijie Qi Liangyi Zhao Yazhou Yuan Jiahui Xu Weikang Rui Katsuhiro Shiratake Jianping Bao Shahrokh Khanizadeh Shaoling Zhang Shutian Tao | 2020 | Horticulture Research2020，7，1： | 3 |
| 13 | Cinnamicaldehyde regulates the expression of tight junction proteins and amino acid transporters in intestinal porcine epithelial cells显示文摘Background: Cinnamicaldehyde(CA) is a key flavor compound in cinnamon essential oil possessing various bioactivities. Tight junction(TJ) proteins are vital for the maintenance of intestinal epithelial barrier function,transport, absorption and utilization of dietary amino acids and other nutrients. In this study, we tested the hypothesis that CA may regulate the expression of TJ proteins and amino acid transporters in intestinal porcine epithelial cells(IPEC-1) isolated from neonatal pigs.Results: Compared with the control, cells incubated with 25 μmol/L CA had increased transepithelial electrical resistance(TEER) and decreased paracellular intestinal permeability. The beneficial effect of CA on mucosal barrier function was associated with enhanced protein abundance for claudin-4, zonula occludens(ZO)-1, ZO-2, and ZO-3. Immunofluorescence staining showed that 25 μmol/L CA promoted the localization of claudin-1 and claudin-3 to the plasma membrane without affecting the localization of other TJ proteins, including claudin-4, occludin,ZO-1, ZO-2, and ZO-3, compared with the control cells. Moreover, protein abundances for rBAT, xCT and LAT2 in IPEC-1 cells were enhanced by 25 μmol/L CA, while that for EAAT3 was not affected.Conclusions: CA improves intestinal mucosal barrier function by regulating the distribution of claudin-1 and claudin-3 in enterocytes, as well as enhancing protein abundance for amino acid transporters rBAT, xCT and LAT2 in enterocytes. Supplementation with CA may provide an effective nutritional strategy to improve intestinal integrity and amino acid transport and absorption in piglets. | Kaiji Sun Yan Lei Renjie Wang Zhenlong Wu Guoyao Wu | 2018 | Journal of Animal Science and Biotechnology2018，9，1： | 3 |
| 14 | A ratiometric benzothiazole-based fluorescence probe for selectively recognizing HClO and its practical applications显示文摘A ratiometric probe(HBT-HBZ)bearing 2-hydrazino benzothiazole and 3-(benzo[d]thiazol-2-yl)-2-hydroxy-5-methylbenzaldehyde for sensing hypochlorous acid(HClO)with high selectivity and sensitivity is reported in this article.The fluorescence intensity ratios(I470nm/I572nm)of the probe with different concentrations of analyte showed excellent selectivity and a linear response to minor changes in HClO.The detection limit of 24 nmol/L suggests that the sensor is very sensitive to HClO.According to the series of performed experiments,HBT-HBZ has practical applications,such as the detection of HClO residues in tap water,which has been rarely reported.In addition,confocal laser microscopy experiments confirmed that HBT-HBZ can selectively recognize HClO in HeLa cells.A ratiometric probe(HBT-HBZ)for sensing HClO with high selectivity and sensitivity is reported in this article.The probe exhibited high selectivity for HClO among other ROS,RNS and anions.In addition,HBTHBZ has some practical applications such as the analysis of the HClO content in tap water.Furthermore,confocal fluorescence microscopy imaging showed that HBT-HBZ can be applied for detecting HClO in living cells. | Kaijie Wang Dazhou Xi Chuantao Liu Yahui Chen Hao Gu Long Jiang Xiaoqiang Chen Fang Wang | 2020 | Chinese Chemical Letters2020，31，11： | 2 |
| 15 | Radiological Characteristics and Anatomical Risk Factors in the Evaluation of Hallux Valgus in Chinese Adults显示文摘 | Hailin Xu Kaiji Jin Zhongguo Fu Minglai Ma Zhongdi Liu Shuai An Baoguo Jiang | 2015 | Chinese Medical Journal2015，，1： | 2 |
| 16 | A novel 3-dimensional micro-ultrasound approach to automated measurement of carotid arterial plaque volume as a biomarker for experimental atherosclerosis显示文摘 | Matthew Walker Barry R. Campbell Karim Azer Christopher Tong Kaijie Fang Jacquelynn J. Cook Michael J. Forrest Kimberly Kempadoo Samuel D. Wright Jeffrey S. Saltzman Euan MacIntyre Richard Hargreaves | 2009 | Atherosclerosis2009，，1： | 1 |
| 17 | Research on the effect of numerical aperture on probing depth of OCT system with new Monte Carlo simulation model显示文摘 | Wu Kaijie Li Gang Lin Ling | 2005 | Progr Biomed Opt Imaging Proc SPIE2005，5630，： | 1 |
| 18 | Characteristic switching of a multilayer th in electrostatic actuator by a driving signal for an ultra-precision motion stage显示文摘 | Mariam Md G Kaiji S | 2013 | Precision Engineer ing2013，37，1： | 1 |
| 19 | Disulfiram, a drug widely used to control alcoholism, suppresses self-renewal of glio- blastoma and overrides resistance to temozolomide 显示文摘 | TRISCOTT J CATHY L KAIJI H | 2012 | Oncotar- get2012，3，10： | 1 |
| 20 | Long-term benefits for the quality of life after video-assisted thoracoscopic lobecto- my in patients with lung cancer显示文摘 | Sugiura H Morikawa T Kaiji M | 1999 | Surg Lap Endo1999，9，6： | 1 |
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