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| 1 | Developmentally Regulated Glucosylation of Bitter Triterpenoid in Cucumber by the UDP-Glucosyltransferase UGT73AM3显示文摘 | Yang Zhong Xiaofeng Xue Zhiqiang Liu Yongshuo Ma Kewu Zeng Lida Han Jingjing Qi Dae-Kyun Ro Soren Bak Sanwen Huang Yuan Zhou Yi Shang | 2017 | Molecular Plant2017，10，7： | 7 |
| 2 | Schisandrol A protects AGEs-induced neuronal cells death by allosterically targeting ATP6V0d1 subunit of V-ATPase显示文摘Diabetes have been shown to cause progressive neuronal injury with pain and numbness via advanced glycation end-products(AGEs)-induced neuronal cell apoptosis;however, the valuable drug targets for diabetic neuropathy have been poorly reported so far. In this study, we discovered a natural small-molecule schisandrol A(SolA) with significant protective effect against AGEs-induced neuronal cell apoptosis. ATP6V0D1, a major subunit of vacuolar-type ATPase(V-ATPase) in lysosome was identified as a crucial cellular target of SolA. Moreover, SolA allosterically mediated ATP6V0D1 conformation via targeting a unique cysteine 335 residue to activate V-ATPase-dependent lysosomal acidification.Interestingly, SolA-induced lysosome pH downregulation resulted in a mitochondrial-lysosomal crosstalk by selectively promoting mitochondrial BH3-only protein BIM degradation, thereby preserving mitochondrial homeostasis and neuronal cells survival. Collectively, our findings reveal ATP6V0D1 is a valuable pharmacological target for diabetes-associated neuronal injury via controlling lysosomal acidification, and also provide the first small-molecule template allosterically activating V-ATPase for preventing diabetic neuropathy. | Xiaoqing Zhou Shaoyang Zhao Tingting Liu Lu Yao Meimei Zhao Xiaoming Ye Xiaowen Zhang Qiang Guo Pengfei Tu Kewu Zeng | 2022 | Acta Pharmaceutica Sinica B2022，12，10： | 3 |
| 3 | Antioxidant,Hypoglycemic and Hypolipidemic Activities of Sambucus williamsii Seed Oil显示文摘[Objectives] To explore the antioxidant activity of Sambucus williamsii seed oil. [Methods]DPPH scavenging method and Prussian blue method( total reducing power) were used. S. williamsii seed oil has antioxidant activity. In the DPPH scavenging free radical experiment,S. williamsii oil showed the ability to scavenge free radicals,and its scavenging ability had linear relationship with oil concentration( R =0. 999 6). Besides,the IC_(50) value of S. williamsii seed oil scavenging DPPH free radicals was 61. 30 ± 0. 88 mg/mL. In the total reducing power measurement experiment,the S. williamsii seed oil has a reducing ability,and its reducing ability is proportional to the oil concentration,and has the concentration dependence. Through studying the inhibitory effect of S. williamsii seed oil on α-glucosidase,the hypoglycemic activity of S. williamsii seed oil was determined. The ability of samples to inhibit α-glucosidase was judged through measuring the absorbance at 400 nm of the reaction system. [Results]In the concentration range of 1. 56-25. 00 mg/mL,the S. williamsii seed oil can effectively inhibit α-glucosidase,and the inhibition rate was 62. 66%-85. 22%. The animal in vivo experiment was used to determine the hypolipidemic activity of the S. williamsii seed oil. First,a high fat modelwas established,S. williamsii seed oil was given through intragastric administration. Then,total cholesterol( TC),triglyceride( TG),high-density lipoprotein cholesterol( HDL-C),and low density lipoprotein cholesterol( LDL-C) were measured. Through comparison of high,medium and low dose groups of S. williamsii seed oil with the high fat modelcontrol group,it was found that the TC,TG,LDL-C levels were significantly decreased( P < 0. 01) and the HDL-C levels were significantly increased( P < 0. 05),indicating that S. williamsii seed oil can effectively reduce the levels of TC,TG,LDL-C in the serum of hyperlipidemic mice,and effectively inhibit the decrease of HDL-C level,thus S. williamsii seed oil can reduce blood lipids in mice,namely,has hypolipidemic effects. In addition,the greater the dose of S. williamsii seed oil,the more obvious the effect of blood lipid levels in mice,indicating that the hypolipidemic effect of S. williamsii seed oil was dose dependent. [Conclusions] S. williamsii seed oil has reducing ability,and there is a significant dose-effect relationship in the concentration range of 2-10 mg/mL. As natural plant oil,S. williamsii seed oil has the advantages of good stability,small toxic and side effects,and strong effect,and has high value of developing new antioxidants. | Wei HU Hui LI Kewu LIU | 2018 | Medicinal Plant2018，9，5： | 2 |
| 4 | Hydrolytic dynamics of pesticide N'-(2,4-dimethylphenyl)-N-methylformamidine in aquatic solution显示文摘HydrolyticdynamicsofpesticideN'-(2,4-dimethylphenyl)-N-methylformamidineinaquaticsolution￥MoHanhong;YangKewu;AnFengchun;LiuYe... | Mo Hanhong Yang Kewu An Fengchun Liu Ye (Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China) | 1996 | Journal of Environmental Sciences1996，8，2： | 1 |
| 5 | Chemical characterization of a new sulfated polysaccharide from Gracilaria chouae and its activation effects on RAW264.7 macrophages显示文摘This study aimed to characterize the chemical composition of a new sulfated polysaccharide from the red alga Gracilaria chouae and evaluate its activation effects on RAW264.7 macrophages.It showed that the obtained G.chouae polysaccharide(GCP-3A)was a sulfated acidic polysaccharide with a molecular weight of 11.87 kDa. | Feifei LI Kehai LIU Kewu LIU | 2022 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2022，23，1： | 1 |
| 6 | Cr(Ⅲ) removal from simulated solution using hydrous magnesium oxide coated fly ash: Optimization by response surface methodology (RSM)显示文摘Hydrous magnesium oxide coated fly ash(MFA) has environmental remediation potential by providing a substrate for the adsorption of aqueous Cr(Ⅲ). Aqueous Cr(Ⅲ) adsorption onto MFA was examined as a function of MFA dosage, p H and initial Cr(Ⅲ) concentration with the Box–Behnken approach used for experimental design and optimization using response surface methodology(RSM). p H and dosage(dosage and concentration) have significant interactive effects on Cr(Ⅲ) adsorption efficiency. Analysis of variance shows that the response surface quadratic model is highly significant and can effectively predict the experimental outcomes. Cr(Ⅲ) removal efficiency of 98% was obtained using optimized conditions of MFA dosage, p H and initial Cr(Ⅲ) concentration of 1.57 g·L^(-1), 4.11 and 126 mg·L^(-1), respectively. Cr(Ⅲ) adsorption onto MFA is mainly attributed to the interaction between Cr(Ⅲ) and the functional group \\OH of the hydrous magnesium oxide, in all probability caused by chemisorptions. The results of this study can conduce to reveal the interactions between Cr(Ⅲ) pollutant and MFA characteristics, posing important implications for the cost-effective alternative adsorption technology in the treatment of heavy metal containing wastewater. | Min Xia Chunsong Ye Kewu Pi Defu Liu Andrea R. Gerson | 2018 | Chinese Journal of Chemical Engineering2018，26，5： | 1 |
| 7 | First-principles study of Sn and Ca doping in CuInO2 显示文摘 | Liu Li Bai Kewu Gong Hao | 2005 | Phys Rev B: Condens Matter Mater Phys2005，72，12： | 1 |
| 8 | Irregular Mapping and its Application in Bit-Inter- leaved LDPC Coded Modulation With Iterative Demapping and Decoding显示文摘 | Zaishuang Liu Kewu Peng Tao Cheng Zhaocheng Wang | 2011 | IEEE Transactions on Broadcasting2011，57，3： | 1 |
| 9 | Speciation analysis of butyltin compounds in Chinese seawater by capillary gas chromatography with flame photometric detection using insitu hydride derivatization followed by headspace solidphase microextraction 显示文摘 | JING Guibin LIU Jiyan YANG Kewu | 2000 | Analytica Chimica Acta2000，421，1： | 1 |
| 10 | Structure and transport mechanism of the human calcium pump SPCA1显示文摘Secretory-pathway Ca^(2+)-ATPases(SPCAs)play critical roles in maintaining Ca^(2+)homeostasis,but the exact mechanism of SPCAsmediated Ca^(2+)transport remains unclear.Here,we determined six cryo-electron microscopy(cryo-EM)structures of human SPCA1(hSPCA1)in a series of intermediate states,revealing a near-complete conformational cycle.With the aid of molecular dynamics simulations,these structures offer a clear structural basis for Ca^(2+)entry and release in hSPCA1.We found that hSPCA1 undergoes unique conformational changes during ATP binding and phosphorylation compared to other well-studied P-type II ATPases.In addition,we observed a conformational distortion of the Ca^(2+)-binding site induced by the separation of transmembrane helices 4L and 6,unveiling a distinct Ca^(2+)release mechanism.Particularly,we determined a structure of the long-sought CaE2P state of P-type IIA ATPases,providing valuable insights into the Ca^(2+)transport cycle.Together,these findings enhance our understanding of Ca^(2+)transport by hSPCA1 and broaden our knowledge of P-type ATPases. | Mengqi Wu Cang Wu Tiefeng Song Kewu Pan Yong Wang Zhongmin Liu | 2023 | Cell Research2023，33，7： | 0 |
| 11 | MAPK介导的YAP激活促进机械力诱导的肺泡再生显示文摘肺泡是肺器官进行血氧交换的基本单位,由两种肺泡上皮细胞组成:进行气体交换的一型肺泡上皮AT1细胞和分泌肺表面活性物质的二型肺泡AT2细胞。 | Zhe Liu Huijuan Wu Kewu Jiang Yanjie Wang Wenjing Zhang Qiqi Chu Juan Li Huanwei Huang Tao Cai Hongbin Ji Chun Yang 汤楠 | 2017 | 科学新闻2017，0，4： | 0 |
| 12 | Economic Value Evaluation of Fine Individual Plants of Ribes rubrum Linn. Based on AHP显示文摘In this experiment,the biological characteristics,the number of spikes per plant,the average number of panicles,the average spike length,and the average yield per plant of the wild Ribes rubrum Linn. were observed and determined and the analytic hierarchy process( AHP)was applied to evaluate the economic value of 8 different sources of excellent plants of R. rubrum Linn. The results indicated that ' RXF02', ' RXF03' and ' RXM05' performed well in the number of spikes per plant,average number of panicles,average yield per plant,and cold resistance,and the comprehensive scores were 9. 68,9. 44 and 9. 41,respectively. | Yu CHEN Yulan GUO Kewu LIU | 2019 | Asian Agricultural Research2019，11，2： | 0 |
| 13 | Anti-psoriasis molecular targets and active components discovery of Optimized Yinxieling Formula via affinity-purified strategy显示文摘Psoriasis,a prevalent inherited skin condition,involves an inflammatory response as a key pathogenic mechanism.The Optimized Yinxieling Formula(OYF),rooted in traditional Chinese medicine,is extensively utilized in clinical settings to treat psoriasis.Although previous studies have demonstrated OYF’s significant anti-inflammatory effects in psoriasis,its potential molecular targets and active components remain unexplored.This study aimed to unveil the anti-psoriasis molecular targets and active components of OYF.Our findings indicated that OYF extract markedly reduced the production of several inflammatory mediators,including IL-23,nitric oxide,TNF-α,and IL-1β,in LPS-induced RAW264.7 cells.We synthesized OYF extract-crosslinked beads to isolate pharmacological targets from RAW264.7 lysates using an affinity purification strategy,known as Target Fishing.The enriched target proteins were subsequently identified via LC-MS/MS,followed by bioinformatics analysis to map the psoriasis-associated pathway-gene network.We identified a total of 76 potential target proteins,which were highly associated with mRNA transcription mechanisms.In particular,pathway-gene network analysis revealed that the IL-23 inflammatory pathway was involved in the anti-psoriasis effect of OYF extract.We further utilized a target protein-based affinity capture strategy,combined with LC-MS and SPR analysis,to globally screen OYF’s active components,focusing on the mRNA transcription regulator,fused in sarcoma(FUS).This process led to the identification of umbelliferone,vanillic acid,protocatechuic acid,gentisic acid,and echinacoside as key compounds targeting FUS to inhibit IL-23 expression.Additionally,we formulated a compound cocktail(CpdC),which significantly reduced psoriasis area and severity index(PASI)scores and the expressions of IL-23 and Ki67 in an imiquimod(IMQ)-induced psoriasis mouse model.Collectively,our study elucidates the primary molecular targets and active components of OYF,offering novel insights for psoriasis treatment. | WANG Wei LIU Lijuan YANG Zhuo LU Chuanjian TU Pengfei ZHAO Ruizhi ZENG Kewu | 2024 | Chinese Journal of Natural Medicines2024，22，2： | 0 |
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