|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | In vivo self-assembled small RNAs as a new generation of RNAi therapeutics显示文摘RNAi therapy has undergone two stages of development,direct injection of synthetic siRNAs and delivery with artificial vehicles or conjugated ligands;both have not solved the problem of efficient in vivo siRNA delivery.Here,we present a proof-of-principle strategy that reprogrammes host liver with genetic circuits to direct the synthesis and self-assembly of siRNAs into secretory exosomes and facilitate the in vivo delivery of siRNAs through circulating exosomes.By combination of different genetic circuit modules,in vivo assembled siRNAs are systematically distributed to multiple tissues or targeted to specific tissues(e.g.,brain),inducing potent target gene silencing in these tissues.The therapeutic value of our strategy is demonstrated by programmed silencing of critical targets associated with various diseases,including EGFR/KRAS in lung cancer,EGFR/TNC in glioblastoma and PTP1B in obesity.Overall,our strategy represents a next generation RNAi therapeutics,which makes RNAi therapy feasible. | Zheng Fu Xiang Zhang Xinyan Zhou Uzair Ur-Rehman Mengchao Yu Hongwei Liang Hongyuan Guo Xu Guo Yan Kong Yuanyuan Su Yangyang Ye Xiuting Hu Wei Cheng Jinrong Wu Yanbo Wang Yayun Gu Sheng-feng Lu Dianqing Wu Ke Zen Jing Li Chao Yan Chen-Yu Zhang Xi Chen | 2021 | Cell Research2021，31，6： | 10 |
| 2 | Antitumor activity of fucoidan against diffuse large B cell lymphoma in vitro and in vivo显示文摘Fucoidan 是从棕色的海草孤立的主要 sulfated 多糖之一。在这研究,我们在弥漫的大 B 房间淋巴瘤(DLBCL ) 上决定了 fucoidan 的反癌症活动在 vitro 并且在 vivo 的房间。Fucoidan 在一个剂量依赖者和时间依赖者举止禁止了 DLBCL 细胞的生长,并且 fucoidan 治疗挑起了 G 0/G1 细胞周期拘捕,它被 p21 伴随起来规定和 cyclin D1, Cdk4,和 Cdk6 下面规定。Fucoidan 也在 DLBCL 房间线和主要 DLBCL 房间导致了 caspase 依赖的房间 apoptosis。另外, fucoidan 治疗从线粒体引起了 mitochondrial 膜潜力的损失和细胞色素 c 和导致 apoptosis 因素的版本进 cytosol。Fucoidan 也加强了在杀死 DLBCL 房间的 carfilzomib 的活动。fucoidan 的口头的管理有效地在异种皮移植老鼠模型禁止了肿瘤生长。我们的调查结果作为一个 anti-DLBCL 代理人揭示 fucoidan 的新奇功能,它能在 DLBCL 的临床的治疗被使用。 | Guang Yang Qianqiao Zhang Yuanyuan Kong Bingqian Xie Minjie Gao Yi Tao Hongwei Xu Fenghuang Zhan Bojie Dai Jumei Shi Xiaosong Wu | 2015 | Acta Biochimica et Biophysica Sinica2015，47，11： | 9 |
| 3 | Integrated metabolo-transcriptomics and functional characterization reveals that the wheat auxin receptor TIR1 negatively regulates defense against Fusarium graminearum显示文摘Fusarium head blight(FHB) caused by Fusarium graminearum Schwabe(teleomorph Gibberella zeae(Schw.) Perch) results in large yield losses in annual global wheat production. Although studies have identified a number of wheat FHB resistance genes,a deeper understanding of the mechanisms underlying host plant resistance to F. graminearum is required for the control of FHB. Here, an integrated metabolomics and transcriptomics analysis of infected wheat plants(Triticum aestivum L.) enabled identification of 789 differentially accumulated metabolites, including flavonoids, phenolamides, tryptamine derivatives, and phytohormones, and revealed altered expression of more than 100 genes that function in the biosynthesis or regulation of these pathways. Our data regarding the effects of F. graminearum infection on flavonoids and auxin signaling led to follow-up experiments that showed that exogenous kaempferide and apigenin application on spikes increased wheat resistance to FHB,while exogenous auxin treatment increased FHB susceptibility. RNAi-mediated knockdown of the gene encoding the auxin receptor, Ta TIR1, increased FHB resistance. Our data supported the use of Ta TIR1 knockdown in controlling FHB. Our study provides insights on the wheat response to F.graminearum infection and its FHB resistance mechanisms while illustrating the potential of Ta TIR1 knockdown in increasing FHB resistance during crop improvement programs. | Peisen Su Lanfei Zhao Wen Li Jinxiao Zhao Jun Yan Xin Ma Anfei Li Hongwei Wang Lingrang Kong | 2021 | Journal of Integrative Plant Biology2021，63，2： | 6 |
| 4 | Deformation analysis of transversely isotropic coal-rock mass with porous and cracks显示文摘Coal-rock as a typical sedimentary rock has obvious stratification,namely it has transversely isotropic feature.Meanwhile,deformation leads to coal-rock mass having the characteristics of different porous and crack structures as well as local anisotropy.Equivalent axial and circumferential strain' formulas of the pure coal-rock mass specimen with a single crack were derived through the establishment of equivalent mechanical model of standard cylindrical coal-rock specimen,and have been widely used to a variety of media combined different structures containing multiple cracks.The complete stress strain curve of a real coal-rock specimen was obtained by the CTC test.Additionally,according to the comparison with the theoretical value,the theoretical mechanical model could well explain the deformation characteristics of coal-rock mass and verify its validity.Further,following features were analyzed:strain normalized coefficient and elastic modulus(Poisson's ratio) in vertical and parallel direction to the stratification,stratification angle,porosity,pore radius,normal and tangential stiffness of crack,and the relationship of different crack width with different tangential stiffness of crack.Through the analysis above,it substantiate this claim that the theoretical model with better reliability reflects the transversely isotropic nature of the coal-rock and the local anisotropy caused by the porous and cracks. | Xue Dongjie Zhou Hongwei Kong Lin Tang Xianli Zhao Tian Yi Haiyang Zhao Yufeng | 2012 | International Journal of Mining Science and Technology2012，22，6： | 3 |
| 5 | Performance evaluation of deep learning-based post-processing and diagnostic reporting system for coronary CT angiography: a clinical comparative study显示文摘To the Editor:Coronary computed tomography angiography(CCTA)has been increasingly,widely performed for diagnosing coronary artery,disease,lAnatomical diagnosis,that is,stenosis grading,is stillthe main diagnostic index provided'by most CCTA tests.Post-processing and interpretation of stenosis are 2 essential'steps that need to be performed bycardiovascular imaging professionals from scan completion to diagnosis conclusion,which is repetitive and time-consuming,taking an average of 30 minutes each case in China and becoming the bottleneck and gradually creating an imbalance between supply and demand.In ine with the rapid development of artificial intelligence(Al)technology in recent years,it has been expected to solve these specific problems.We developed an AI system for automating post-processing and diagnostic reporting of CCTA data using deep learning algorithms to establishanew1-clickworkflowforeverydayuse,namely,CCTA-AI(Figure 1).To further assess its capabilities,this study intends to answer 2 following questions:To what extent can it improve the efficiency of post-processing?To what extent can CCTA-AI detect and calculate coronary artery stenosis due to each atherosclerotic plaque? | Nan Luo Yi He Jitao Fan Ning Guo Guang Yang Yuanyuan Kong Jianyong Wei Tao Bi Jie Zhou Jiaxin Cao Xianjun Han Fang Li Shiyu Zhang Rujing Sun Zhaozhao Wang Tian Ma Lixue Xu Hui Chen Hongwei Li Zhenchang Wang Zhenghan Yang | 2022 | Chinese Medical Journal2022，135，19： | 2 |
| 6 | Two-Stage Over-the-Air (OTA) Test Method for LTE MIMO Device Performance Evaluation显示文摘 | Ya Jing Xu Zhao Hongwei Kong Steve Duffy Moray Rumney Ryan J. Pirkl | 2012 | International Journal of Antennas and Propagation2012，，： | 1 |
| 7 | Plasma phospholipid metabolic profiling and biomarkers of mouse IgA nephropathy显示文摘 | LEWEN JIA CHANG WANG HONGWEI KONG | 2006 | Metabolomics2006，2，2： | 1 |
| 8 | Suppressing Al2O3 nanoparticle coarsening and Cu nanograin growth of milled nanostructured Cu-5vol.%Al2O3 composite powder particles by doping with Ti显示文摘Both the coarsening of Al_2O_3 nanoparticles and the growth of Cu nanograins of mechanically milled nanostructured Cu-5 vol.%Al_2O_3 composites with, and without, trace amounts of Ti during annealing at973 K for 1 h were investigated. It was found that doping with a small amount of Ti(e.g. 0.2 wt%) in a nanostructured Cu-5 vol.%Al_2O_3 composite effectively suppressed the coarsening of Al_2O_3 nanoparticles during exposure at this temperature. Further, the Ti addition also prevented the concomitant abnormal growth of the copper grains normally caused by the coarsening of the Al_2O_3 nanoparticles. Energy dispersive X-ray spectroscopy analysis of the Al_2O_3 nanoparticles in the annealed Cu-5 vol.%Al2 O3-0.2 wt%Ti sample suggested that the Ti atoms either diffused into the Al_2O_3 nanoparticles or segregated to the Cu/Al_2O_3 interfaces to form Ti-doped Al_2O_3 nanoparticles, which was more stable than Ti-free Al_2O_3 nanoparticles during annealing at high homologous temperatures. | Dengshan Zhou Hongwei Geng Wei Zeng Deliang Zhang Charlie Kong Paul Munroe | 2017 | Journal of Materials Science & Technology2017，33，11： | 1 |
| 9 | et al ,Congestion Control Algorithm for Resilient Packet Ring 显示文摘 | KONG Hongwei Ge Ning RUAN Fang | 2003 | TSINGHUA SCIENCE AND TECHNOLOGY2003，8，： | 1 |
| 10 | Analysis of cigarette smoke condensates by comprehensive two-dimensional gas chromatography/time-of-flight mass spectrometry (GC X GC/TOFMS) 显示文摘 | LU Xin CAI Junlan KONG Hongwei | 2003 | Anal Chem2003，75，17： | 1 |
| 11 | Effect of PA-MSHA vaccine on plasma phospholipids metabolic profi- ling and the ratio of Th2/Thl cells within immune organ oI mouse IgA nephropathy显示文摘 | Lewen Jia Chang Wang Hongwei Kong | 2007 | Journal of Pharmaceutical and Biomedical Analysis2007，，43： | 1 |
| 12 | Differences between the metabolic profiles of decompensated and compensated cirrhosis patients with Hepatitis B virus infections under high-performance liquid chromatography-mass spectrometry显示文摘 | Yu Chen Zhiliang Xu Hongwei Kong Nan Chen Jing Chen Lina Zhou Feiling Wang Yuejiao Dong Shufa Zheng Zhenjing Chen Guowang Xu Lanjuan Li | 2012 | Metabolomics2012，，5： | 1 |
| 13 | Characterization of complex hydrocarbons in cigarette smoke condensate by gas chromatography-mass spectrometry and comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry显示文摘 | LU XIN ZHAO MINGYUE KONG HONGWEI | 2004 | J Chromat A2004，1043，： | 1 |
| 14 | Impacts of hydrogen dilution on growth and optical properties of a-SiC:H films显示文摘Hydrogenated amorphous silicon-carbon (a-SiC:H) films were deposited by plasma enhanced chemical vapor deposition (PECVD) with a fixed methane to silane ratio ([CH4]/[SiH4]) of 1.2 and a wide range of hydrogen dilution (RH=[H2]/[SiH4 + CH4]) values of 12, 22, 33, 102 and 135. The impacts of RH on the structural and optical properties of the films were investigated by using UV-VIS transmission, Fourier transform infrared (FTIR) absorption, Raman scattering and photoluminescence (PL) measur- ements. The effects of high temperature annealing on the films were also probed. It is found that with increasing hydrogen dilution, the optical band gap increases, and the PL peak blueshifts from ~1.43 to 1.62 Ev. In annealed state, the room temperature PL peak for the low RH samples disappears, while the PL peak for the high RH samples appears at ~2.08 Ev, which is attributed to nanocrystalline Si particles confined by Si-C and Si-O bonds. | HU Zhihua LIAO Xianbo DIAO Hongwei KONG Guanglin ZENG Xiangbo XU Yanyue | 2004 | Science China(Technological Sciences)2004，47，3： | 1 |
| 15 | In situ Carbon Modification of g-C3N4 from Urea co-Crystal with Enhanced Photocatalytic Activity Towards Degradation of Organic Dyes Under Visible Light显示文摘An in sitt strategy was introduced for synthesizing carbon modified graphitic carbon nitride(g-C3N4)by using urea/4-aminobenzoic acid(PABA)co-crystal(PABA@Urea)as precursor materials.Via co-calcination of the PABA co-former and the urea in PABA@a Urea co-crystals,C guest species were generated and compounded into g-C3Na matrix in situ by replacing the lattice N of the carbon nitride and forming carbon dots onto its layer surface.The carbon modification dramatically enhanced visible-light harvesting and charge carrier separation.Therefore.visible light photo-catalytic oxidation of methylene blue(MB)pollution in water over the carbon modified g-C3Na(C/g-C3Na)was notably improved.Up to 99%of methylene blue(MB)was eliminated within 60 min by the optima sample prepared from the PABA@Urea co-crystal with a PABA content of 0.1%(mass ratio),faster than the degrada-tion rate over bare g-C3N4.The present study demonstrates a new way to boost up the photocatalysis performance of g-C3Na,which holds great potential concerning the degradation of organic dyes from water. | ZHAO Weifeng HAO Ning ZHANG Gai MA Aijie CHEN Weixing ZHOU Hongwei YANG Dong XU Ben Bin KONG Jie | 2020 | Chemical Research in Chinese Universities2020，36，6： | 1 |
| 16 | A novel strategy to evaluate the quality of traditional Chinese medicine based on the correlation analysis of chemical finge print and biological effect显示文摘 | Wang Jing Kong Hongwei Yuan Zimin | 2013 | J Pharm Biomed Anal2013，，83： | 1 |
| 17 | Characterization of cigarette smoke condensates by comprehensive two-dimensional gas chromatography/Time-of-Flight mass spectrometry (GC × GC-TOFMS): basic fraction显示文摘 | Lu Xin Zhao Mingyue Kong Hongwei | 2004 | J Sep Sci2004，27，： | 1 |
| 18 | Characterization of complex hydrocarbons in cigarette smoke condensate by gas chromatography-mass spectrometry and comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry 显示文摘 | Lu Xin Zhao Mingyue Kong Hongwei | 2004 | J Chromatogr A2004，1043，： | 1 |
| 19 | Two-Stage Over-the-Air (OTA) Test Method for LTE MIMO Device Performance Evaluation显示文摘 | Ya Jing Xu Zhao Hongwei Kong Steve Duffy Moray Rumney Ryan J. Pirkl | 2012 | International Journal of Antennas and Propagation2012，，： | 1 |
| 20 | A GC-based metabonomics investigation of type 2 diabetes by organic acids metabolic profile显示文摘 | YUAN Kailong KONG Hongwei GUAN Yufeng | 2007 | J Chromatogr B Analyt Teehnol Biomed Life Sei2007，850，12： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员