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| 1 | Homology-mediated end joining-based targeted integration using CRISPR/Cas9显示文摘 | Yao, Xuan Wang, Xing Hu, Xinde Liu, Zhen Liu, Junlai Zhou, Haibo Shen, Xiaowen Wei, Yu Huang, Zijian Ying, Wenqin Wang, Yan Nie, Yan-Hong Zhang, Chen-Chen Li, Sanlan Cheng, Leping Wang, Qifang Wu, Yan Huang, Pengyu Sun, Qiang Shi, Linyu Yang, Hui | 2017 | Cell Research2017，27，6： | 28 |
| 2 | One-step generation of complete gene knockout mice and monkeys by CRISPR/Cas9-mediated gene editing with multiple sgRNAs显示文摘CRISPR/Cas9 系统是一个有效编辑基因的方法,但是编辑基因的动物的多数显示出 mosaicism,与编辑仅仅在房间的部分发生。这里,我们证明那单个基因或多重基因能被 Cas9 mRNA 和多重邻近的单个指南的 RNA 的 zygotic 注射完全在老鼠和猴子胚胎击倒(spaced 10-200 bp 分开) 指向仅仅一把单个钥匙每基因的 exon。个别地在 Y 染色体上跟随八基因的指向的删除的 F0 老鼠的 Phenotypic 分析在产生猛烈老鼠表明了这条途径的坚韧性。重要地,这条途径在高效率交付完全的基因大美人(Arntl 上的 100% 并且 91% 在 Prrt2 上) 在猴子胚胎。最后,我们能在一个单个步骤产生一只完全的 Prrt2 猛烈猴子,表明在很快建立编辑基因的猴子模型的这条途径的实用性。 | Erwei Zuo Yi-Jun Cai Kui Li Yu Wei Bang-An Wang Yidi Sun Zhen Liu Jiwei Liu Xinde Hu Wei Wei Xiaona Huo Linyu Shi Cheng Tang Dan Liang Yan Wang Yan-Hong Nie Chen-Chen Zhang Xuan Yao Xing Wang Changyang Zhou Wenqin Ying Qifang Wang Ren-Chao Chen Qi Shen Guo-Liang Xu Jinsong Li Qiang Sun Zhi-Qi Xiong Hui Yang | 2017 | Cell Research2017，27，7： | 21 |
| 3 | Microstructure and properties of as-cast Cu-Cr-Zr alloys with lanthanum addition显示文摘Cu-0.45 Cr-0.2 Zr-xLa(x = 0-0.48) alloys were prepared by vacuum casting. The effects of La addition and orientation on the microstructure and properties of the as-cast alloy were investigated by an optical microscope, a scanning electron microscope with an energy dispersive X-ray spectrometer, a tensile testing machine and an electrical conductivity tester. The result shows that the addition of La significantly refines the columnar grainsize and decreases the secondary dendrite arm spacing. Trace addition of La improves the room temperature ultimate tensile strength,elongation and electrical conductivity mainly by purifying during melting and casting. The ultimate tensile strength, elongation and electrical conductivity significantly decrease with the increase of La content due to formation of coarse particles and oxides, which severely harm the performance of the Cu-0.45 Cr-0.2 Zr-xLa alloys. The Cu-0.45 Cr-0.2 Zr-0.13 La alloy possesses a good combination of room temperature ultimate tensile strength, elongation and electrical conductivity. In addition, room temperature ultimate tensile strength and electrical conductivity along transverse direction of the ingot are higher than that along longitudinal direction,which is mainly ascribed to different distribution of grain boundary and grain orientation. | Jilin Li Lili Chang Shengli Li Xinde Zhu Zhongxin An | 2018 | Journal of Rare Earths2018，36，4： | 6 |
| 4 | MOF-derived Co-MOF,O-doped carbon as trifunctional electrocatalysts to enable highly efficient Zn-air batteries and water-splitting显示文摘Development of high-efficiency non-noble electrocatalysts for oxygen reduction reaction(ORR),oxygen evolution reaction(OER)and hydrogen evolution reaction(HER)is urgently needed for high-performance Zn-air batteries and overall water splitting.Here,a facile strategy to synthesize novel Co-MOF,O-doped carbon(Co-MOF-T)based on Zn,Co-doped glucosamine and ZIF-8 by pyrolysis at temperature T was demonstrated.The prepared Co-MOF-800 showed a superior oxygen reduction reaction(ORR)activity comparable to that of commercial Pt/C catalyst.In addition,this catalyst shows great potential in the overall water splitting due to the excellent oxygen evolution reaction(OER)and hydrogen evolution reaction(HER)activities.Based on the trifunctional activity,the primary Zn-air batteries using a Co-MOF-800 air electrode achieved a high open-circuit voltage of 1.38 V,a specific capacity of 671.6 mAh g^(-1) Zn,and a prominent peak power density of 144 mW cm^(-2).Also,the rechargeable Zn-air batteries based on CoMOF-800 air electrode could be smoothly run for 510 cycles with a low voltage gap of 0.58 V.Finally,the trifunctional Co-MOF-800 catalyst was applied to boost the electrochemical water splitting,demonstrating its promising potential as a green energy material for practical applications. | Xinde Duan Na Pan Can Sun Kexin Zhang Xukun Zhu Mingdao Zhang Li Song Hegen Zheng | 2021 | Journal of Energy Chemistry2021，30，5： | 5 |
| 5 | Base editing-mediated splicing correction therapy for spinal muscular atrophy显示文摘Dear Editor,Spinal muscular atrophy(SMA)is a devastating autosomal recessive motor neuron disease.1,2 Infants with more severe forms of type I SMA die before the age of 2 if no intervention is provided.1,2 Spinraza and zolgensma have been approved by the FDA as SMA therapeutics for pediatric patients.1,2 However,as an antisense oligonucleotide(ASO)based therapy,spinraza requires four loading doses,followed by three annual maintenance doses.The patients would be subjected to repeated intrathecal injections in the procedure.Zolgensma is a single-dose gene-replacement therapy for SMA,but unfortunately is unreliable in maintaining a high,stable level of gene expression.These issues have limited the therapeutic effects of the two approved drugs for SMA. | Xiang Lin Haizhu Chen Ying-Qian Lu Shunyan Hong Xinde Hu Yanxia Gao Lu-Lu Lai Jin-Jing Li Zishuai Wang Wenqin Ying Lixiang Ma Ning Wang Erwei Zuo Hui Yang Wan-Jin Chen | 2020 | Cell Research2020，30，6： | 5 |
| 6 | Treatment of autosomal recessive hearing loss via in vivo CRISPR/Cas9-mediated optimized homology-directed repair in mice显示文摘Dear Editor,Hearing loss is the most common sensory disorder in the world.Among cases of non-syndromic hearing loss,which account for 70%of all cases of genetic hearing loss,around 80%of cases arise from autosomal recessive loss-of-function mutations that require repair,rather than disruption,of the mutant allele.1 CRISPR/Cas9-mediated homology-directed repair(HDR)-based therapies have the potential to cure many genetic diseases because this class of therapeutics can achieve arbitrary base changes as well as the insertion or deletion of DNA fragments/However. | Xi Gu Xinde Hu Daqi Wang Zhijiao Xu Fang Wang Di Li Geng-lin Li Hui Yang Huawei Li Erwei Zuo Yilai Shu | 2022 | Cell Research2022，32，7： | 3 |
| 7 | AN APPROXIMATE REASONING METHOD IN DEZERT-SMARANDACHE THEORY显示文摘With the increment of focal elements number in discernment framework,the computation amount in Dezert-Smarandache Theory (DSmT) will exponentially go up. This has been the bottleneck problem to block the wide application and development of DSmT. Aiming at this difficulty,in this paper,a kind of fast approximate reasoning method in hierarchical DSmT is proposed. Presently,this method is only fit for the case that there are only singletons with assignment in hyper-power set. These singletons in hyper-power set are forced to group through bintree or tri-tree technologies. At the same time,the assignments of singletons in those different groups corresponding to each source are added up respectively,in order to realize the mapping from the refined hyper-power set to the coarsened one. And then,two sources with the coarsened hyper-power set are combined together according to classical DSm Combination rule (DSmC) and Proportional Conflict Redistribution rule No. 5 (PCR5). The fused results in coarsened framework will be saved as the connecting weights between father and children nodes. And then,all assignments of singletons in different groups will be normalized respectively. Tree depth is set,in order to decide the iterative times in hierarchical system. Finally,by comparing new method with old one from different views,the superiority of new one over old one is testified well. | Li Xinde Wu Xuejian Sun Jiaming Meng Zhengda | 2009 | Journal of Electronics(China)2009，26，6： | 3 |
| 8 | A novel network composite cathode of LiFePO4/multiwalled carbon nanotubes with high rate capability for lithium ion batteries 显示文摘 | Li Xinlu Kang Feiyu Bai Xinde | 2007 | Electrochemistry Communications2007，，9： | 1 |
| 9 | Unified fusion approach with application to SLAM显示文摘 | LI Xinde HUANG Xin-han WANG Min | 2006 | International Journal of Intelligent Technology2006，1，1： | 1 |
| 10 | Evidence Supporting Mmeasure of Similarity for Reducing the Complexity in Information Funsion显示文摘 | Xinde Li Jean Dezert Florentin Smarandache | 2011 | Information Sciences2011，，： | 1 |
| 11 | Evidence supporting measure of similarity for reducing the complexity in informa- tion fusion显示文摘 | Li Xinde Dezert J Smarandache F | 2011 | Information Sciences2011，181，10： | 1 |
| 12 | Fusion of imprecise qualitative information显示文摘 | Xinde Li Xianzhong Dai Jean Dezert Florentin Smarandache | 2010 | Applied Intelligence2010，，3： | 1 |
| 13 | Mirror writing in elderly patients with Alzheimer disease and vascular dementia显示文摘BACKGROUND:The results showed that mirror writing(MW)was correlated with the development of written language,so that MW examination may be one of methods to examine the intelligence of elderly people.OBJECTIVE:To study the MW in elderly patients with Alzheimer disease(AD)and vascular dementia(VaD)and take appropriate scale for their evaluation.DESIGN:Taking the written portion of the Chinese Aphasia Examination Scale(1994)for assessment.SETTING:Department of Neurology,Neuropsychological Laboratory,Beijing Hospital.PARTICIPANTS:From March 1998 to January 2001,33 patients with AD,30 patients with VaD admitted into Department of Neurology,Beijing Hospital was enrolled into study.Criteria according to the Diagnostic and Statistical Manual of Mental Disorder,4th edition(DSM-IV),published by the American Psychiatric Association was used to diagnose AD,while criteria according to the National Institute of Neurological Disorders and Stroke-Association Internationale pour la Recherche et l' Enseignement en Neurosciences(NINDS-AIREN)and Alzheimer Disease Diagnostic and Treatment Center(ADDCT)were used for diagnosis of VaD.AD group contained 19 males and 14 females aged 60-83 years.Twenty-eight males and 2 females,aged 60-87 years made up the VaD group.The 63 healthy elderly subjects matched on age and education as controls were enrolled into study.The matched controls were categorized AD control(n =33)and VaD control(n =30).All patients and controls were understanding and agree with all items of assessment.METHODS:MW examination,Mini Mental State Examination(MMSE),Hachinski Ischemic Scale,the Global Deterioration Scale(GDS)were examined in all subjects.① Use the written potion of the Chinese Aphasia examination Scale(1994),patient using MW for 91%-100% of dictation had complete MW,those using MW for 51%-90% of dictation had severe MW,those using MW for 11%-50% had moderate MW,those using MW for 1%-10% had mild MW.② According to the MMSE,the patients were considered to have dementia if they were illiterate and had an MMSE score ≤ 17 score or educated time ≤ 6 years and MMSE ≤ 20 score or educated time > 6 years and MMSE ≤ 24 score.③ Using the Hachinski Ischemic Scale to differentiate the AD and VaD,it included 6 items and total 9 scores.> 7 score was VaD,< 4 score was AD and 4-7 score was blended dementia.④ Using the GDS to assess cognitive function:Standard criteria were divided in 7 degrees:1 degree:no impairment of cognition and 7 degree:very severe impairment of cognition.MAIN OUTCOME MEASURES:The data of MW examination,evaluation of MMSE,Hachinski Ischemic Scale and the GDS of all assessed subjects.RESULTS:All 63 cases of AD and VaD and 63 healthy controls were entered for analysis.①Results of MW examination:A total of 17 patients with AD were characterized as using MW,3 with moderate MW and 14 patients with mild MW.In the corresponding control group,only 2 subjects were characterized as being mild MW.The VaD group has 23 patients with MW,2 with moderate and 21 patients with mild MW.② MMSE score:MMSE score of AD group was much lower than that of individuals in control group(20.15± 3.40),(29.73±0.40)score,P < 0.01,MMSE score of VaD group was much lower than that of individuals in control group(19.33±2.75),(29.12±0.63)score,P < 0.01.③ Hachinski Ischemic Scale and GDS score:Rating according to the Hachinski Ischemic Scale was higher in VaD patients compared to AD patients(9.61 ±1.69),(1.09±0.60)score,P < 0.01.The GDS score did not significantly differ between the AD group and the VaD group.CONCLUSION:① MW examination could be used as an indicator of intelligence in healthy elderly people and also could be used as one of methods to assess the intelligence in AD and VaD patients.② Grade of severity of MW may indirectly reflect the degree of dementia. | Xinde Wang Dantao Peng Xiaojie Cai Shuhua Li | 2007 | Neural Regeneration Research2007，2，4： | 1 |
| 14 | Short-term effects of raw rice straw and its derived biochar on greenhouse gas emission in five typical soils in China显示文摘 | Feiyue Li Xinde Cao Ling Zhao Fan Yang Jianfei Wang Shuwei Wang | 2013 | Soil Science and Plant Nutrition2013，，5： | 1 |
| 15 | Disruption of splicing-regulatory elements using CRISPR/Cas9 to rescue spinal muscular atrophy in human iPSCs and mice显示文摘We here report a genome-editing strategy to correct spinal muscular atrophy(SMA).Rather than directly targeting the pathogenic exonic mutations,our strategy employed Cas9 and guide-sg RNA for the targeted disruption of intronic splicing-regulatory elements.We disrupted intronic splicing silencers(ISSs,including ISS-N1 and ISS+100)of survival motor neuron(SMN)2,a key modifier gene of SMA,to enhance exon 7 inclusion and full-length SMN expression in SMA iPSCs.Survival of splicing-corrected iPSC-derived motor neurons was rescued with SMN restoration.Furthermore,co-injection of Cas9 mRNA from Streptococcus pyogenes(SpCas9)or Cas9 from Staphylococcus aureus(SaCas9)alongside their corresponding sgRNAs targeting ISS-N1 into zygotes rescued 56%and 100%of severe SMA transgenic mice(Smn-/-,SMN2tg/-).The median survival of the resulting mice was extended to>400 days.Collectively,our study provides proof-of-principle for a new strategy to therapeutically intervene in SMA and other RNA-splicing-related diseases. | Jin-Jing Li Xiang Lin Cheng Tang Ying-Qian Lu Xinde Hu Erwei Zuo He Li Wenqin Ying Yidi Sun Lu-Lu Lai Hai-Zhu Chen Xin-Xin Guo Qi-Jie Zhang Shuang Wu Changyang Zhou Xiaowen Shen Qifang Wang Min-Ting Lin Li-Xiang Ma Ning Wang Adrian R.Krainer Linyu Shi Hui Yang Wan-Jin Chen | 2020 | National Science Review2020，7，1： | 1 |
| 16 | Synthesis of liquid-crystalline polyacrylates with laterally substituted mesogens显示文摘 | Zhou Qifeng Li Huimin Feng Xinde | 1987 | Macromolecules1987，20，1： | 1 |
| 17 | Immobilization of Zn, Cu, and Pb in contaminated soils using phosphate rock and phosphoric acid显示文摘 | Xinde Cao Ammar Wahbi Lena Ma Bing Li Yongliang Yang | 2008 | Journal of Hazardous Materials2008，，2： | 1 |
| 18 | Solubilities of N--acrylamide in meth- anol-ethanol solution显示文摘 | Yao Xinding Li Yanxun Luo Tingliang | 2009 | Fluid Phase Equilibria2009，288，12： | 1 |
| 19 | 显示文摘 | Tang Xinde Zhang Qizhen Li Aixiang | 2004 | Chin J Chem2004，22，： | 1 |
| 20 | Solubilities of N - - acrylamide in methanol - ethanol solution显示文摘 | Yao Xinding Li Yanxun Luo Tingliang | 2010 | Fluid Phase Equilibria2010，288，12： | 1 |
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