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| 1 | Lower Bifidobacteria counts in both duodenal mucosa-associated and fecal microbiota in irritable bowel syndrome patients显示文摘AIM: To determine the composition of both fecal and duodenal mucosa-associated microbiota in irritable bowel syndrome (IBS) patients and healthy subjects using molecular-based techniques. METHODS: Fecal and duodenal mucosa brush samples were obtained from 41 IBS patients and 26 healthy subjects. Fecal samples were analyzed for the composition of the total microbiota using fluorescent in situ hybridization (FISH) and both fecal and duodenal brush samples were analyzed for the composition of bif idobacteria using real-time polymerase chain reaction. RESULTS: The FISH analysis of fecal samples revealed a 2-fold decrease in the level of bifidobacteria (4.2 ± 1.3 vs 8.3 ± 1.9, P < 0.01) in IBS patients compared to healthy subjects, whereas no major differences in other bacterial groups were observed. At the species level, Bifidobacterium catenulatum levels were significantly lower (6 ± 0.6 vs 19 ± 2.5, P < 0.001) in the IBS patients in both fecal and duodenal brush samples than in healthy subjects.CONCLUSION: Decreased bifidobacteria levels in both fecal and duodenal brush samples of IBS patients compared to healthy subjects indicate a role for microbiotic composition in IBS pathophysiology. | Angèle PM Kerckhoffs Melvin Samsom Michel E van der Rest Joris de Vogel Jan Knol Kaouther Ben-Amor Louis MA Akkermans | 2009 | World Journal of Gastroenterology2009，15，23： | 33 |
| 2 | Different antibiotic susceptibility between antrum and corpus of the stomach,a possible reason for treatment failure of Helicobacter pylori infection显示文摘AIM:To assess whether antibiotic resistance varies between the antrum and corpus of the stomach of patients that are either Helicobacter pylori(H.pylori)therapy-naive or pre-treated.METHODS:H.pylori strains were isolated from antrum and corpus biopsies from 66 patients that received a diagnostic gastroduodenoscopy for variant clinical indications.Antimicrobial susceptibility to amoxicillin,clarithromycin,tetracycline,metronidazole,levofloxacin and rifabutin was tested with the E-test method on IsoSensitest agar with 10 vol%defibrinated horse blood.In patients with a different antibiotic susceptibility pattern between the isolates from the antrum and corpus,DNA fingerprinting via random amplified polymorphic DNA analysis was performed to detect differences among DNA patterns of H.pylori isolates.RESULTS:Primary,secondary and tertiary resistance to clarithromycin was 6.9%,53.8%and 83.3%,retrospectively.Metronidazole and levofloxacin resistance also increased according to the number of previous treatments(17.2%,69.2%,83.3%;13.8%,23.1%,33.3%).Tertiary resistance to rifabutin was detected in12.5%of patients.In none of the 66 patients a resistance against amoxicillin or tetracycline was detectable.Discordant antibiotic susceptibility between antrum and corpus isolates for different antibiotics was seen in 15.2%(10/66)of the patients.Two out of those ten patients were naive to any H.pylori antibiotic treatment.The remaining eight patients previously received at least one eradication therapy.DNA fingerprinting analysis revealed no substantial differences among DNA patterns between antrum and corpus isolates in the majority of patients suggesting an infection with a single H.pylori strain.CONCLUSION:Different antibiotic susceptibility between antrum and corpus biopsies is a common phenomenon and a possible explanation for treatment failure.Resistant H.pylori strains may be missed if just one biopsy from one anatomic site of the stomach is taken for H.pylori susceptibility testing. | Michael Selgrad Ina Tammer Cosima Langner Jan Bornschein Julia Mei?le Arne Kandulski Mariya Varbanova Thomas Wex Dirk Schlüter Peter Malfertheiner | 2014 | World Journal of Gastroenterology2014，20，43： | 10 |
| 3 | Value of a newly sequenced bacterial genome显示文摘Next-generation sequencing(NGS) technologies have made high-throughput sequencing available to medium- and small-size laboratories, culminating in a tidal wave of genomic information. The quantity of sequenced bacterial genomes has not only brought excitement to the field of genomics but also heightened expectations that NGS would boost antibacterial discovery and vaccine development. Although many possible drug and vaccine targets have been discovered, the success rate of genome-based analysis has remained below expectations. Furthermore, NGS has had consequences for genome quality, resulting in an exponential increase in draft(partial data) genome deposits in public databases. If no further interests are expressed for a particular bacterial genome, it is more likely that the sequencing of its genome will be limited to a draft stage, and the painstaking tasks of completing the sequencing of its genome and annotation will not be undertaken. It is important to know what is lost when we settle for a draft genome and to determine the 'scientific value' of a newly sequenced genome. This review addresses the expected impact of newly sequenced genomes on antibacterial discovery and vaccinology. Also, it discusses the factors that could be leading to the increase in the number of draft deposits and the consequent loss of relevant biological information. | Eudes GV Barbosa Flavia F Aburjaile Rommel TJ Ramos Adriana R Carneiro Yves Le Loir Jan Baumbach Anderson Miyoshi Artur Silva Vasco Azevedo | 2014 | World Journal of Biological Chemistry2014，5，2： | 7 |
| 4 | Sequencing a Juglans regia×J.microcarpa hybrid yields high-quality genome assemblies of parental species显示文摘Members of the genus Juglans are monecious wind-pollinated trees in the family Juglandaceae with highly heterozygous genomes,which greatly complicates genome sequence assembly.The genomes of interspecific hybrids are usually comprised of haploid genomes of parental species.We exploited this attribute of interspecific hybrids to avoid heterozygosity and sequenced an interspecific hybrid Juglans microcarpa×J.regia using a novel combination of single-molecule sequencing and optical genome mapping technologies.The resulting assemblies of both genomes were remarkably complete including chromosome termini and centromere regions.Chromosome termini consisted of arrays of telomeric repeats about 8 kb long and heterochromatic subtelomeric regions about 10 kb long.The centromeres consisted of arrays of a centromere-specific Gypsy retrotransposon and most contained genes,many of them transcribed.Juglans genomes evolved by a whole-genome-duplication dating back to the Cretaceous-Paleogene boundary and consist of two subgenomes,which were fractionated by numerous short gene deletions evenly distributed along the length of the chromosomes.Fractionation was shown to be asymmetric with one subgenome exhibiting greater gene loss than the other.The asymmetry of the process is ongoing and mirrors an asymmetry in gene expression between the subgenomes.Given the importance of J.microcarpa×J.regia hybrids as potential walnut rootstocks,we catalogued disease resistance genes in the parental genomes and studied their chromosomal distribution.We also estimated the molecular clock rates for woody perennials and deployed them in estimating divergence times of Juglans genomes and those of other woody perennials. | Tingting Zhu Le Wang Frank M.You Juan C.Rodriguez Karin R.Deal Limin Chen Jie Li Sandeep Chakraborty Bipin Balan Cai-Zhong Jiang Patrick J.Brown Charles ALeslie Mallikarjuna KAradhya Abhaya M.Dandekar Patrick E.McGuire Daniel Kluepfel Jan Dvorak Ming-Cheng Luo | 2019 | Horticulture Research2019，6，1： | 7 |
| 5 | Liver Transplantation for Neuroendocrine Tumors in Europe—Results and Trends in Patient Selection: A 213-Case European Liver Transplant Registry Study显示文摘 | Yves Patrice Le Treut Emilie Grégoire Jürgen Klempnauer Jacques Belghiti Elisabeth Jouve Jan Lerut Denis Castaing Olivier Soubrane Olivier Boillot Georges Mantion Kia Homayounfar Manuel Bustamante Daniel Azoulay Philippe Wolf Marek Krawczyk Andreas Pasche | 2013 | Annals of Surgery2013，，5： | 3 |
| 6 | Cardiac dysfunction and cell damage across clinical stages of severity in growth-restricted fetuses显示文摘 | Fatima Crispi Edgar Hernandez-Andrade Maurice M.A.L. Pelsers Walter Plasencia Jesus Andres Benavides-Serralde Elisenda Eixarch Ferdinand Le Noble Asif Ahmed Jan F.C. Glatz Kypros H. Nicolaides Eduard Gratacos | 2008 | American Journal of Obstetrics and Gynecology2008，，3： | 1 |
| 7 | Transcriptional Activation of Apolipoprotein CIII Expression by Glucose May Contribute to Diabetic Dyslipidemia显示文摘 | Sandrine Caron An Verrijken Ilse Mertens Carolina Huaman Samanez Gisèle Mautino Joel T. Haas Daniel Duran-Sandoval Janne Prawitt Sven Francque Emmanuelle Vallez Anne Muhr-Tailleux Isabelle Berard Folkert Kuipers Jan A. Kuivenhoven Sudha B. Biddinger Marja | 2011 | Arteriosclerosis Thrombosis and Vascular Biology2011，，3： | 1 |
| 8 | Angiopoietin- like 4 is a proangiogenic factor produced during ischemia and in conventional renal cell carcinoma显示文摘 | Se'bastien Le Jan Ce'line Amy Aure'lie Cazes | 2003 | Am J Pathol2003，162，5： | 1 |
| 9 | wastewater particles in laboratory biofilm reactors under anoxic and Water Science & Technology, 1998, Hydrolysis of organic scale and pilot scale aerobic conditions 显示文摘 | Janning KF Le TX Harremos P | 1998 | 38(8/9) : 179-1881998，38，89： | 1 |
| 10 | A complex adenovirus vaccine against chikungunya virus provides complete protection against viraemia and arthritis显示文摘 | Danher Wang Andreas Suhrbier Adam Penn-Nicholson Jan Woraratanadharm Joy Gardner Min Luo Thuy T. Le Itaru Anraku Michael Sakalian David Einfeld John Y. Dong | 2011 | Vaccine2011，，15： | 1 |
| 11 | Angiopoietin-like 4 prevents metastasis through inhibition of vascular permeability and tumor cell motility and invasiveness 显示文摘 | Galaup A Cazes A Le Jan S | 2006 | Proc Natl Acad Sci USA2006，103，18： | 1 |
| 12 | Angiopoietin-like 4 is a proangiogenie factor produced during isehemia and in conventional renal cell carcinoma 显示文摘 | Le Jan S Amy C Cazes A | 2003 | Am J Pathol2003，162，5： | 1 |
| 13 | Characterization of the expression of the hypoxia-indueed genes neuritin, TXNIP and IGFBP3 in cancer显示文摘 | Ire Jan S Le Meur N Cazes A | 2006 | FEBS Lett2006，580，14： | 1 |
| 14 | Characterization of the expression of the hypoxia-induced genes neuritin,TXNIP and IGFBP3 in cancer显示文摘 | Le Jan S Le Meur N Cazes A | | 0，，14： | 1 |
| 15 | Characterization of the expression of the hypoxia-induced genes neuritin, TXNIP and IGFBP3 in cancer显示文摘 | Sébastien Le Jan Nolwenn Le Meur Aurélie Cazes Josette Philippe Martine Le Cunff Jean Léger Pierre Corvol Stéphane Germain | 2006 | FEBS Letters2006，，14： | 1 |
| 16 | Modeling toxicity of binary metal mixtures(Cu2+-Ag+,Cu2+-Zn2+)to lettuce,Lactuca sativa,with the biotic ligand model显示文摘 | Yen Le T T Vijver M G Jan Hendriks A | 2013 | Environmental Toxicology and Chemistry2013，32，1： | 1 |
| 17 | Hypersensitivity to soybean protein in early weaned piglets, preliminary observations 显示文摘 | Dreau D Lalles J P Le Jan | 1992 | Nutr Clin Metabol1992，6，： | 1 |
| 18 | 挪威Storgama小型源头集水区降水量操控实验:对有机碳和氮化物淋溶的影响显示文摘挪威南部气候的预期变化包括夏秋降水量的增加。这会影响土壤溶解有机物(DOM)的淋溶。本文评估了Storgama(59°0′N,海拔550~600m)小型源头集水区3年(2004~2006年)生长季中实验性增加降水(10mm/周)对总有机碳(TOC)和总有机氮(TON)淋溶的影响。额外降水(extra precipitation)对径流中TOC和TON平均浓度没有显著影响。所以,TOC和TON通量的增加几乎与水流量成比例。这说明,集水区土壤储存的吸附性和潜在移动性TOC和TON缓冲了径流中的DOM浓度。TOC和TON库的大小和动态依赖于生产和淋溶速度之间的平衡。罕见的短暂干旱对来自参照流域径流中的TOC和TON通量只有很小的影响。 | Stle Haaland Kari Austnes Φyvind Kaste Jan Mulder Gunnhild Riise Live Semb Vestgarden Arne O.Stuanes 王胜 | 2008 | AMBIO-人类环境杂志2008，37，1： | 1 |
| 19 | The risk of myocardial infarction in patients with reduced activity of cytochrome P450 2C9 显示文摘 | Visser LE van Schaik RH Jan Danser AH | 2007 | Pharmacogenet Genomics2007，17，7： | 1 |
| 20 | Identifying risk factors for eggshell contamination by Bacillus cereus group bacteria in French laying farms显示文摘 | A.Z. Kone S. Jan C. Le Marechal N. Grosset M. Gautier J. Puterflam F. Baron | 2013 | British Poultry Science2013，，3： | 1 |
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