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| 1 | Pachytene piRNAs instruct massive mRNA elimination during late spermiogenesis显示文摘在哺乳动物的精子发生被 piRNA 表示的二个波浪描绘:一个人对应于负责的经典 piRNAs 因为 silencing retrotransponsons 和第二个波浪主要在 pachytene spermatocytes 从 nontransposon intergenic 区域被导出,但是这些 pachytene piRNAs 的功能大部分是未知的。这里,我们在在老鼠伸长指示巨大的 mRNA 消除报导 pachytene piRNAs 的参与 spermatids (ES ) 。我们证明包含鼠科的 PIWI (MIWI ) 和 deadenylase CAF1 的导致 piRNA 的 silencing 建筑群(pi-RISC ) 有选择地在 ES 被装配,它负责经由在体的房间类似于 miRNAs 的行动的机制导致 mRNA deadenylation 和腐烂。如此的一个高度安排的节目看起来充分利用 pachytene piRNAs 的能力从 nontransposon intergenic 区域导出的多样化的指向的庞大的全部剧目。这些调查结果建议 pachytene piRNAs 负责为精子生产作准备,从 ES 使广阔细胞的节目失去活性。 | Lan-Tao Gou Peng Dai Jian-Hua Yang Yuanchao Xue Yun-Ping Hu Yu Zhou Jun-Yan Kang Xin Wang Hairi Li Min-Min Hua Shuang Zhao Si-Da Hu Li-Gang Wu Hui-Juan Shi Yong Li Xiang-Dong Fu Liang-Hu Qu En-Duo Wang Mo-Fang Liu | 2014 | Cell Research2014，24，6： | 39 |
| 2 | From concept to reality-A review to the primary test stand and its preliminary application in high energy density physics显示文摘Pulsed power technology,whereas the electrical energy stored in a relative long period is released in much shorter timescale,is an efficient method to create high energy density physics(HEDP)conditions in laboratory.Around the beginning of this century,China Academy of Engineering Physics(CAEP)began to build some experimental facilities for HEDP investigations,among which the Primary Test Stand(PTS),a multi-module pulsed power facility with a nominal current of 10 MA and a current rising time~90 ns,is an important achievement on the roadmap of the electro-magnetically driven inertial confinement fusion(ICF)researches.PTS is the first pulsed power facility beyond 10 TW in China.Therefore,all the technologies have to be demonstrated,and all the engineering issues have to be overcome.In this article,the research outline,key technologies and the preliminary HEDP experiments are reviewed.Prospects on HEDP research on PTS and pulsed power development for the next step are also discussed. | Jianjun Deng Weiping Xie Shuping Feng Meng Wang Hongtao Li Shengyi Song Minghe Xia Ji Ce An He Qing Tian Yuanchao Gu Yongchao Guan Bin Wei Xianbin Huang Xiaodong Ren Jiakun Dan Jing Li Shaotong Zhou Hongchun Cai Siqun Zhang Kunlun Wang Qiang Xu Yujuan Wang Zhaohui Zhang Guilin Wang Shuai Guo Yi He Yiwei Zhou Zhanji Zhang Libing Yang Wenkang Zou | 2016 | Matter and Radiation at Extremes2016，1，1： | 19 |
| 3 | Selective separation of protein and saccharides by ionic liquids aqueous two-phase systems显示文摘In the present work,it was found that aqueous solution of a hydrophilic ionic liquid (IL),1-butyl-3-methylimidazolium dicyanamide ([C4mim][N(CN)2]),could be separated into an aqueous two-phase system (ATPS) by inorganic salts such as K2HPO4 and K3PO4.The top phase is IL-rich,while the bottom phase is phosphate-rich.It was shown that 82.7%-100% bovine serum albumin (BSA) could be enriched into the top phase and almost quantitative saccharides (arabinose,glucose,sucrose,raffinose or dextran) were preferentially extracted into the bottom phase in a single-step extraction by [C4mim][N(CN)2] + K2HPO4 ATPS.The extraction efficiency of BSA from the aqueous saccharide solutions was influenced by the molecular structure of saccharides.The conductivity,dynamic light scattering (DLS) and transmission electron microscopy (TEM) were combined to investigate the microstructure of the IL-rich top phase and the possible mechanism for the selective separation.It is suggested that the formation of the IL aggregate and the IL aggregate-BSA complex plays a significant role in the separation of BSA from aqueous saccharide solutions.This is the first example for the selective separation by ILs-based ATPSs.It is expected that these findings would have potential applications in bio-analysis,separation,and IL recycle. | PEI YuanChao LI ZhiYong LIU Li WANG JianJi WANG HuiYong | 2010 | Science China Chemistry2010，53，7： | 12 |
| 4 | Phytophthora sojae Effector PsAvh240 Inhibits Host Aspartic Protease Secretion to Promote Infection显示文摘Plants secrete defense molecules into the extracellular space (the apoplast) to combat attacking microbes. However, the mechanisms by which successful pathogens subvert plant apoplastic immunity remain poorly understood. In this study, we show that PsAvh240, a membrane-localized effector of the soybean pathogen Phytophthora sojae, promotes P. sojae infection in soybean hairy roots. We found that PsAvh240 interacts with the soybean-resistant aspartic protease GmAP1 in planta and suppresses the secretion of GmAP1 into the apoplast. By solving its crystal structure we revealed that PsAvh240 contain six a helices and two WY motifs. The first two a helices of PsAvh240 are responsible for its plasma membrane-localization and are required for PsAvh240's interaction with GmAP1. The second WY motifs of two PsAvh240 molecules form a handshake arrangement resulting in a handshake-like dimer. This dimerization is required for the effector's repression of GmAP1 secretion. Taken together, these data reveal that PsAvh240 localizes at the plasma membrane to interfere with GmAP1 secretion, which represents an effective mechanism by which effector proteins suppress plant apoplastic immunity. | Baodian Guo Haonan Wang Bo Yang Wenjing Jiang Maofeng Jing Haiyang Li Yeqiang Xia Yuanpeng Xu Qinli Hu Fangfang Wang Feng Yu Yan Wang Wenwu Ye Suomeng Dong Weiman Xing Yuanchao Wang | 2019 | Molecular Plant2019，12，4： | 12 |
| 5 | Design and development of an oral remdesivir derivative W116 against SARS-CoV-2显示文摘Dear Editor,Since the declaration of COVID-19 as a global pandemic on March 11,2020,this pandemic has been circulating for 17 months throughout the world,leading to more than 200 million infections and nearly 4.4 million deaths as of August 15,2021.The pathogen of COVID-19 is a novel coronavirus named SARS-CoV-2,which shares-79%genome sequence identity with SARS-CoV.1 At the early stage of the COVID-19 outbreak,5ARS-CoV-2 caused great panic in the hardest-hit areas due to its high transmissibility and pathogenicity.To fight the COVID-19 crisis,drug repurposing was immediately pursued in order to find potential therapeutics. | Yuanchao Xie Wanchao Yin Yumin Zhang Weijuan Shang Zhen Wang Xiaodong Luan Guanghui Tian Haji A.Aisa Yechun Xu Gengfu Xiao Jia Li Hualiang Jiang Shuyang Zhanq Leike Zhang H.Eric Xu Jingshan Shen | 2021 | Cell Research2021，31，11： | 12 |
| 6 | Performance of ecological restoration in an impaired coral reef in the Wuzhizhou Island, Sanya, China显示文摘Coral restoration is becoming popular to help restoring degraded coral reefs.However,few studies have tried to monitor the long-term recovery of coral reefs,which makes it diffi cult to assess the performance of the restoration.We monitored the growth of three transplanted Acropora corals and naturally-attached Pocillopora damicornis on artifi cial reefs(ARs)from October 2014 to September 2018 during which there were several attacks of typhoons.Results show that two staghorn Acropora species had the highest growth rates(11.0–12.1 cm/a),followed by table coral A.divaricate(5.6 cm/a)and P.damicornis(4.8 cm/a).A linear growth pattern was found for the three Acropora species;the pattern gradually slowed in P.damicornis.There was a strong interspecifi c competition for space among the corals on ARs,and it led to the sharply declined occurrence of slow-growing P.darmicornis colonies in 2017.Coral recovery was successful at the Wuzhizhou Island and quickly increased AR complexity.However,the ARs made of metal frames fail to resist the direct attack from a catastrophic typhoon.Therefore,concrete and environmentalfriendly materials should be used in future restoration.This study is the fi rst report on long-term monitoring and assessment of coral reef restoration in China.The results off er future guide of reef restoration for impaired coral reefs in regions easily aff ected by typhoons. | Xinqing ZHENG Yuanchao LI Jilin LIANG Rongcheng LIN Daoru WANG | 2021 | Journal of Oceanology and Limnology2021，39，1： | 6 |
| 7 | A new distinct geminivirus causes soybean stay-green disease显示文摘Dear Editor,Plant viruses make up almost half of the plant disease-causing pathogens,affecting crop yields and the global economy(Savary et al.,2019).Soybean(Glycine max)is one of the most valuable legume crops in the world,supplying 25%of the global edible oil and two-thirds of the global concentrated protein for livestock feeding.Recently,the outbreak of soybean stay-green syndrome with delayed leaf senescence(stay-green),flat pods,and increased number of abnormal seeds has swept the soybean production in the Huang-Huai-Hai region of China,resulting in huge yield losses(Xu et al.,2019).This disease has become an epidemic and prominent problem in soybean production and is still expanding its geography,including North America,posing a serious threat to soybean production(Harbach et al.,2016;Zhang et al.,2016;Li et al.,2019).However,the cause of soybean stay-green syndrome remains obscure. | Ruixiang Cheng Ruoxin Mei Rong Yan Hongyu Chen Dan Miao Lina Cai Jiayi Fan Gairu Li Ran Xu Weiguo Lu Yu Gao Wenwu Ye Shuo Su Tianfu Han Junyi Gai Yuanchao Wang Xiaorong Tao Yi Xu | 2022 | Molecular Plant2022，15，6： | 6 |
| 8 | Thermal stability of LiFePO_4/C-LiMn_2O_4 blended cathode materials显示文摘Safety is important to lithium ion battery materials. The thermal stability of LiFePO_4/C-LiMn_2O_4 blended cathode materials is characterized by using TG, XRD, and SEM etc. The results show that LiFePO_4/C-LiMn_2O_4 possesses a worse thermal stability than pure spinel LiMn_2O_4 and pure olivine LiFePO_4/C. When LiFePO_4/C-LiMn_2O_4 blended cathode materials are sintered at 500°C under Ar atmosphere, the sintered cathode materials emit O_2, and appear impurity phases(Li_3PO_4, Fe_2O_3, Mn_3O_4). It is deduced that some chemical reactions take place between different materials, which leads to a worse discharge specific capacity. LiFePO_4/C-LiMn_2O_4 blended cathode materials, therefore, need to be managed and controlled strictly for the sake of thermal stability and safety. | DU YuanChao HUANG XiaoPeng ZHANG KeYu LIANG Feng LI QiuXia YAO YaoChun DAI YongNian | 2017 | Science China(Technological Sciences)2017，60，1： | 5 |
| 9 | The RNA-binding protein ROD1/PTBP3 cotranscriptionally defines AID-loading sites to mediate antibody class switch in mammalian genomes显示文摘 | Juan Chen Zhaokui Cai Meizhu Bai Xiaohua Yu Chao Zhang Changchang Cao Xihao Hu Lei Wang Ruibao Su Di Wang Yingpeng Yao Rong Ye Baidong Hou Yang Yu Shuyang Yu Jinsong Li Yuanchao xue | 2018 | Cell Research2018，28，10： | 4 |
| 10 | Anionic structural effect in liquid–liquid separation of phenol from model oil by choline carboxylate ionic liquids显示文摘Phenolic compounds exist in crude oil as pollutants, and their removal is vital important for the refining and further application of oils. In traditional separation approaches, strong acid and strong base have to be used to remove these compounds, which may cause serious environmental problems. In this work, 19 kinds of cholinium ionic liquids have been developed to separate phenol from model oil by liquid–liquid extraction. Structural effect of anions of the ionic liquids in the separation is systematically investigated. It is found that depending on the chemical structure of ionic liquids, phenol can be removed from toluene with single-step removal efficiency from 86 to 99% under optimal conditions. The type of substituent groups and the-CH_2 number between two carboxylates have obvious effect on the removal efficiency, and more hydrophilic ionic liquids have a stronger extraction performance for phenol. Furthermore, thermodynamic,^(13) C NMR,~1 H NMR and density functional theory calculations have been performed to characterize the extraction process and to understand the extraction mechanism. It is shown that the extraction of phenol from oil to ionic liquid is a favorable process, and this process is mainly driven by enthalpy change. The formation of the hydrogen bond between anion of the ionic liquid and-OH of phenol is the main driving force for the extraction of phenol from oil to the ionic liquids. | Zhiyong Li Ruipeng Li Xiaoqing Yuan Yuanchao Pei Yuling Zhao Huiyong Wang Jianji Wang | 2019 | Green Energy & Environment2019，4，2： | 4 |
| 11 | Geochemistry of ore-forming fluids and geological significance of the Kuoerzhenkuola gold field in Xinjiang, China显示文摘The Kuoerzhenkuola gold field (including the Kuoerzhenkuola and the Buerkesidai gold deposits) is the most important one in the Sawuer gold belt, northern Xinjiang, China. Iso-topic studies including D, O, He, C, S, Pb and Sr reveal that the ore-forming fluids of the Kuo-erzhenkuola and the Buerkesidai deposits shared the same source: the water of ore fluids was magmatic water and minor meteoric water; the mineralizers and ore materials derived mainly from mantle beneath the island arc, and partially from crust. The ore-forming fluids of two depos-its are a mixture of mantle-derived fluids incorporated by crust-derived fluid, and meteoric water. Based on these results, combined with the consideration of the tectonic setting and geological features, we suggest that the two gold deposits in the Kuoerzhenkuola gold field, Sawur gold belt share the same genesis, and are volcanogenic hydrothermal gold deposits occurring in the same caldera. | SHEN Ping SHEN Yuanchao LIU Tiebing LI Guangming ZENG Qingdong | 2005 | Science China Earth Sciences2005，48，11： | 3 |
| 12 | SRSF1 plays a critical role in invariant natural killer T cell development and function显示文摘Invariant natural killer T(iNKT)cells are highly conserved innate-like T lymphocytes that originate from CD4^(+)CD8^(+)double-positive(DP)thymocytes.Here,we report that serine/arginine splicing factor 1(SRSF1)intrinsically regulates iNKT cell development by directly targeting Myb and balancing the abundance of short and long isoforms.Conditional ablation of SRSF1 in DP cells led to a substantially diminished iNKT cell pool due to defects in proliferation,survival,and TCRαrearrangement.The transition from stage 0 to stage 1 of iNKT cells was substantially blocked,and the iNKT2 subset was notably diminished in SRSF1-deficient mice.SRSF1 deficiency resulted in aberrant expression of a series of regulators that are tightly correlated with iNKT cell development and iNKT2 differentiation,including Myb,PLZF,Gata3,ICOS,and CD5.In particular,we found that SRSF1 directly binds and regulates pre-mRNA alternative splicing of Myb and that the expression of the short isoform of Myb is substantially reduced in SRSF1-deficient DP and iNKT cells.Strikingly,ectopic expression of the Myb short isoform partially rectified the defects caused by ablation of SRSF1.Furthermore,we confirmed that the SRSF1-deficient mice exhibited resistance to acute liver injury uponα-GalCer and Con A induction.Our findings thus uncovered a previously unknown role of SRSF1 as an essential post-transcriptional regulator in iNKT cell development and functional differentiation,providing new clinical insights into iNKT-correlated disease. | Jingjing Liu Menghao You Yingpeng Yao Ce Ji Zhao Wang Fang Wang Di Wang Zhihong Qi Guotao Yu Zhen Sun Wenhui Guo Juanjuan Liu Shumin Li Yipeng Jin Tianyan Zhao Hai-Hui Xue Yuanchao Xue Shuyang Yu | 2021 | Cellular & Molecular Immunology2021，18，11： | 3 |
| 13 | The Effect of Anti-Human CD134 Monoclonal Antibody on Phytohemagglutinin-Induced mRNA Expression of Perforin in Peripheral Blood Mononuclear Cells显示文摘CD134, a member of the tumor necrosis factor receptor superfamily, plays a crucial role in T cell survival. In this study, peripheral blood mononuclear cells (PBMCs) stimulated with phytohemagglutinin (PHA, 50 μg/ml) were treated by CD134 mAb (1 μg/ml, 5 μg/ml, 10 μg/ml) for 6 h, 12 h, 24 h and 48 h. The level of perforin mRNA was measured by reverse transcription-polymerase chain reaction (RT-PCR) technique. Our data showed that the expression of perforin mRNA in PBMCs was down-regulated by CD134 mAb in a dose-dependent manner in range of 1 μg/ml to 5 μg/ml and dropped down to its minimum on 24 h (p < 0.05). The level of perforin mRNA reached a plateau when the concentration of CD134 mAb exceeded 5 μg/ml. In conclusion, CD134 mAb can inhibit perforin expression, which may enhance the ability of T cells for survival. | Ming Li~1 Yuanchao Zhang~(1,2) ~1Department of Rheumatology and Immunology,Shandong Provincial Hospital,Shandong University,Jinan 250021,China ~2Department of Rheumatology and Immunology,Shandong Provincial Hospital,Shandong University,Jinan 250021,Shandong,China. | 2005 | Cellular & Molecular Immunology2005，2，6： | 2 |
| 14 | Effects of Different Extraction and Purification Methods on the Acquisition of Phycoerythrin from Porphyridium purpureum显示文摘Phycoerythrin, as the main light-harvesting antenna in Porphyridium purpureum, exists at the outermost end of the phycobilisome. It has advantages of good fluorescence intensity, anti-oxidation, scavenging free radicals, and high chroma, so it has been widely used in food, cosmetics, pharmaceuticals, and other industries. In this study, the effects of different extraction(ultrasonic breaking method, bead grinding method, liquid nitrogen grinding method, and freezing-thawing method) and purification methods(salting out method, ultrafiltration method, and combination of salting out and ultrafiltration method) on the acquisition of phycoerythrin from P. purpureum were studied, and the characteristics of phycoerythrin in the P. purpureum were identified. The results showed that the freezing-thawing method could extract phycoerythrin from the powder of P. purpureum to the utmost extent, and the concentration of the extracted phycoerythrin was up to 0.036 g/L. The salting out method could most effectively purify phycoerythrin, and the purity index was 2.216. The identification of phycoerythrin by ultraviolet absorption spectroscopy and fluorescence spectroscopy indicated that the phycoerythrin had the maximum absorption peak at 545 nm, and the maximum Stokes shift was up to 79 nm. Due to its high fluorescence characteristics, it can be used as a fluorescent marker in the fields of molecular biology and clinical medicine, and can also be used as a good photosensitizer in tumor therapy. | Yuanchao XU Shengxin NIE Zhen LIU Jinglong LI | 2019 | Agricultural Biotechnology2019，8，2： | 2 |
| 15 | Oral remdesivir derivative VV116 is a potent inhibitor of respiratory syncytial virus with efficacy in mouse model显示文摘Dear Editor,Respiratory syncytial virus(RSV)is the leading cause of serious lower respiratory tract disease in children under 5 years of age worldwide,causing an estimated 3.2 million hospitalizations and 120,000 deaths in children globally per year.Furthermore,nearly all children can be infected with RSV by 2 years of age,and individuals can be repeatedly re-infected with RSV throughout life,which poses great threats to infants,the elderly. | Ruxue Zhang Yumin Zhang Wei Zheng Weijuan Shang Yan Wu Ning Li Jun Xiong Hualiang Jiang Jingshan Shen Gengfu Xiao Yuanchao Xie Leike Zhang | 2022 | Signal Transduction and Targeted Therapy2022，7，5： | 2 |
| 16 | Northwestern Junggar Basin, Xiemisitai Mountains, China: A geochemical and geochronological approach显示文摘 | Ping Shen Yuanchao Shen Xian-Hua Li Hongdi Pan Heping Zhu Lei Meng Huawu Dai | 2012 | LITHOS2012，，： | 1 |
| 17 | Sturgeon aquaculture in China:progress,strategies and prospects assessed on the basis of nationwide surveys(2007-2009)显示文摘 | WEI Qiwei ZOU Yuanchao LI P | | 0，，02： | 1 |
| 18 | MicroRNA Directly Enhances Mitochondrial Translation during Muscle Differentiation显示文摘 | Xiaorong Zhang Xinxin Zuo Bo Yang Zongran Li Yuanchao Xue Yu Zhou Jie Huang Xiaolu Zhao Jie Zhou Yun Yan Huiqiong Zhang Peipei Guo Hui Sun Lin Guo Yi Zhang Xiang-Dong Fu | 2014 | Cell2014，，： | 1 |
| 19 | Recent advances in RNA structurome显示文摘RNA structures are essential to support RNA functions and regulation in various biological processes. Recently, a range of novel technologies have been developed to decode genome-wide RNA structures and novel modes of functionality across a wide range of species. In this review, we summarize key strategies for probing the RNA structurome and discuss the pros and cons of representative technologies. In particular, these new technologies have been applied to dissect the structural landscape of the SARS-CoV-2 RNA genome. We also summarize the functionalities of RNA structures discovered in different regulatory layers-including RNA processing, transport, localization, and mRNA translation-across viruses, bacteria, animals, and plants. We review many versatile RNA structural elements in the context of different physiological and pathological processes(e.g., cell differentiation, stress response, and viral replication). Finally, we discuss future prospects for RNA structural studies to map the RNA structurome at higher resolution and at the single-molecule and single-cell level, and to decipher novel modes of RNA structures and functions for innovative applications. | Bingbing Xu Yanda Zhu Changchang Cao Hao Chen Qiongli Jin Guangnan Li Junfeng Ma Siwy Ling Yang Jieyu Zhao Jianghui Zhu Yiliang Ding Xianyang Fang Yongfeng Jin Chun Kit Kwok Aiming Ren Yue Wan Zhiye Wang Yuanchao Xue Huakun Zhang Qiangfeng Cliff Zhang Yu Zhou | 2022 | Science China(Life Sciences)2022，65，7： | 1 |
| 20 | Design of environmentally friendly ionic liquid aqueous two-phase systems for the efficient and high activity extraction of proteins显示文摘 | Zhiyong Li Xinxin Liu Yuanchao Pei | 2012 | Green Chem2012，14，： | 1 |
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