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| 1 | 5-Hydroxymethylcytosine signatures in circulating cell-free DNA as diagnostic biomarkers for human cancers显示文摘象 5-methylcytosine (5mC ) 和 5-hydroxymethylcytosine (5hmC ) 那样的 DNA 修正是知道在哺乳动物影响全球基因表示的 epigenetic 标记。在人的染色体,与基因表示的靠近的关联和高化学的稳定性给他们的流行,这些 DNA epigenetic 标记能为癌症用作理想的 biomarkers 诊断。利用一种高度敏感、选择的化学标记技术,我们这里报导在传播没有房间的 DNA ( cfDNA )并且在从最近与 colorectal 诊断的 260 个病人的一个队收集的配对的肿瘤和邻近的纸巾的 genomic DNA ( gDNA )的 5hmC 的染色体宽的介绍,胃,胰腺,从 90 个健康个人的肝或甲状腺癌症和正常纸巾。5hmC 主要在与开的染色质和容许的 histone 修正重合的 transcriptionally 活跃的区域被散布。柔韧的联系癌症的 5hmC 签名在 cfDNA 被识别为特定的癌症类型是特征的。传播 cfDNA 的 5hmC 底 biomarkers colorectal 和胃的癌症是高度预兆的并且比常规 biomarkers 优异并且比得上从织物活体检视的 5hmC biomarkers。因此,这新策略能从血样品的分析为癌症的诊断和预后导致有效、最低限度地侵略的方法的发展。 | Wenshuai Li Xu Zhang Xingyu Lu Lei You Yanqun Song Zhongguang Luo Jun Zhang Ji Nie Wanwei Zheng Diannan Xu Yaping Wang Yuanqiang Dong Shulin Yu Jun Hong Jianping Shi Hankun Hao Fen Luo Luchun Hua Peng Wang Xiaoping Qian Fang Yuan Lianhuan Wei Ming Cui Taiping Zhang Quan Liao Menghua Dai Ziwen Liu Ge Chen Katherine Meckel Sarbani Adhikari Guifang Jia Marc B Bissonnette Xinxiang Zhang Yupei Zhao Wei Zhang Chuan He Jie Liu | 2017 | Cell Research2017，27，10： | 30 |
| 2 | Combined detection of serum tumor markers for differential diagnosis of solid lesions located at the pancreatic head显示文摘BACKGROUND: The differential diagnosis of solid lesions located at the pancreatic head is very important for choosing therapies and setting up surgical tactics. This study was designed to evaluate the clinical significance of combined measurement of multiple serum tumor markers and the application of the receiver-operating characteristic (ROC) curves in the differential diagnosis of solid lesions located at the pancreatic head. METHODS: The serum levels of CA19-9, CA242, CA50 and carcinoembryonic antigen (CEA) in 112 patients with carcinoma of the pancreatic head and 38 patients with focal chronic pancreatitis in the pancreatic head were measured with ELISA. The sensitivity, specificity, positive likelihood ratio (PLR) and negative likelihood ratio (NLR) of the four serum tumor markers were calculated. The ROC curves for the four serum tumor markers were constructed and the area under the curve (AUC) was calculated. RESULTS: The AUCs of CA19-9, CA242, CA50 and CEA were 0.805, 0.749, 0.738 and 0.705; the PLRs were 1.91, 3.43, 5.09 and 5.46; and the NLRs were 0.41, 0.56, 0.59 and 0.71, respectively. Combined measurements increased the diagnostic specificity, and parallel combined testing increased the diagnostic sensitivity. CONCLUSIONS: Combined measurement of serum tumor markers CA19-9, CA242, CA50 and CEA is valuable in differential diagnosis of solid lesions located at the pancreatic head, and CA19-9 has the best diagnostic ability. Combined measurements can increase the specificity ofdiagnosis. Evaluation with the ROC curve is better than the sensitivity or specificity alone and the results are more integrated and objective. | Liao, Quan Zhao, Yu-Pei Yang, Ying-Chi Li, Li-Jun Long, Xiao Han, Shao-Mei | 2007 | Hepatobiliary & Pancreatic Diseases International2007，6，6： | 27 |
| 3 | Single-cell RNA-seq highlights intra-tumoral heterogeneity and malignant progression in pancreatic ductal adenocarcinoma显示文摘Pancreatic ductal adenocarcinoma (PDAC) is the most common type of pancreatic cancer featured with high intra-tumoral heterogeneity and poor prognosis. To comprehensively delineate the PDAC intra-tumoral heterogeneity and the underlying mechanism for PDAC progression, we employed single-cell RNA-seq (scRNA-seq) to acquire the transcriptomic atlas of 57,530 individual pancreatic cells from primary PDAC tumors and control pancreases, and identified diverse malignant and stromal cell types, including two ductal subtypes with abnormal and malignant gene expression profiles respectively, in PDAC. We found that the heterogenous malignant subtype was composed of several subpopulations with differential proliferative and migratory potentials. Cell trajectory analysis revealed that components of multiple tumor-related pathways and transcription factors (TFs) were differentially expressed along PDAC progression. Furthermore, we found a subset of ductal cells with unique proliferative features were associated with an inactivation state in tumor-infiltrating T cells, providing novel markers for the prediction of antitumor immune response. Together, our findings provide a valuable resource for deciphering the intra-tumoral heterogeneity in PDAC and uncover a connection between tumor intrinsic transcriptional state and T cell activation, suggesting potential biomarkers for anticancer treatment such as targeted therapy and immunotherapy. | Junya Peng Bao-Fa Sun Chuan-Yuan Chen Jia-Yi Zhou Yu-Sheng Chen Hao Chen Lulu Liu Dan Huang Jialin Jiang Guan-Shen Cui Ying Yang Wenze Wang Dan Guo Menghua Dai Junchao Guo Taiping Zhang Quan Liao Yi Liu Yong-Liang Zhao Da-Li Han Yupei Zhao Yun-Gui Yang Wenming Wu | 2019 | Cell Research2019，29，9： | 24 |
| 4 | Pancreatic duct stones in patients with chronic pancreatitis:surgical outcomes显示文摘BACKGROUND: Pancreatic duct stone (PDS) is a common complication of chronic pancreatitis. Surgery is a common therapeutic option for PDS. In this study we assessed the surgical procedures for PDS in patients with chronic pancreatitis at our hospital. METHOD: Between January 2004 and September 2009, medical records from 35 patients diagnosed with PDS associated with chronic pancreatitis were retrospectively reviewed and the patients were followed up for up to 67 months. RESULTS: The 35 patients underwent ultrasonography, computed tomography, or both, with an overall accuracy rate of 85.7%. Of these patients, 31 underwent the modified Puestow procedure, 2 underwent the Whipple procedure, 1 underwent simple stone removal by duct incision, and 1 underwent pancreatic abscess drainage. Of the 35 patients, 28 were followed up for 4-67 months. There was no postoperative death before discharge or during follow-up. After the modified Puestow procedure, abdominal pain was reduced in patients with complete or incomplete stone clearance (P>0.05). Steatorrhea and diabetes mellitus developed in several patients during a long-term follow-up. CONCLUSIONS: Surgery, especially the modified Puestow procedure, is effective and safe for patients with PDS associated with chronic pancreatitis. Decompression of intraductal pressure rather than complete clearance of all stones predicts postoperative outcome. | Liu, Bo-Nan Zhang, Tai-Ping Zhao, Yu-Pei Liao, Quan Dai, Meng-Hua Zhan, Han-Xiang | 2010 | Hepatobiliary & Pancreatic Diseases International2010，9，4： | 14 |
| 5 | Biomaterial-based strategies for maxillofacial tumour therapy and bone defect regeneration显示文摘Issues caused by maxillofacial tumours involve not only dealing with tumours but also repairing jaw bone defects.In traditional tumour therapy,the systemic toxicity of chemotherapeutic drugs,invasive surgical resection,intractable tumour recurrence,and metastasis are major threats to the patients’lives in the clinic.Fortunately,biomaterial-based intervention can improve the efficiency of tumour treatment and decrease the possibility of recurrence and metastasis,suggesting new promising antitumour therapies.In addition,maxillofacial bone tissue defects caused by tumours and their treatment can negatively affect the physiological and psychological health of patients,and investment in treatment can result in a multitude of burdens to society.Biomaterials are promising options because they have good biocompatibility and bioactive properties for stimulation of bone regeneration.More interestingly,an integrated material regimen that combines tumour therapy with bone repair is a promising treatment option.Herein,we summarized traditional and biomaterial-mediated maxillofacial tumour treatments and analysed biomaterials for bone defect repair.Furthermore,we proposed a promising and superior design of dual-functional biomaterials for simultaneous tumour therapy and bone regeneration to provide a new strategy for managing maxillofacial tumours and improve the quality of life of patients in the future. | Bowen Tan Quan Tang Yongjin Zhong Yali Wei Linfeng He Yanting Wu Jiabao Wu Jinfeng Liao | 2021 | International Journal of Oral Science2021，13，1： | 9 |
| 6 | Laparoscopic distal pancreatectomy with or without splenectomy: spleen-preservation does not increase morbidity显示文摘BACKGROUND: The indications for laparoscopic spleen-preserving distal pancreatectomy (LSPDP) and its morbidity compared with laparoscopic distal pancreatectomy with splenectomy (LDPS) are ill-defined. This study aimed to share the indications for spleen-preservation and investigate the safety and outcome of LSPDP at our institution. METHODS: A retrospective review of patients who were scheduled to receive laparoscopic surgery for distal pancreatic lesions was conducted. The indications, surgical procedures, intra-operative data, and outcomes of the two procedures were collected and compared by statistical analysis. RESULTS: LDPS and LSPDP were successfully performed in 16 and 21 patients respectively, whereas they were converted to open surgery in 9 patients. There were no significant differences in age, gender, operation time, blood loss, and conversion rate between the LDPS and LSPDP groups. The mean tumor size showed an inter-group difference (5.05 vs 2.53 cm, P<0.001). There were no significant differences in complication and morbidity rates between the two groups. All patients remained alive without recurrence during a follow-up of 9 to 67 months (median 35).CONCLUSION: LSPDP has a morbidity and outcome comparable to LDPS. | Yu-Pei Zhao, Xiao Du, Meng-Hua Dai, Tai-Ping Zhang, Quan Liao, Jun-Chao Guo, Lin Cong ,Ge Chen Department of General Surgery, Peking Union Medical College Hospital,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100730, China | 2012 | Hepatobiliary & Pancreatic Diseases International2012，11，5： | 8 |
| 7 | Simulation of phase transition process using lattice Boltzmann method显示文摘A new lattice Boltzmann model based on SC model, is proposed to describe the liquid-vapor phase transitions. The new model is validated through the simulation of the one-component phase transition process. Compared with the simulation results of van der Waals gas and the Maxwell equal-area construction, the results of the new model have a better agreement with the analytical solutions than those of SC and Zhang models. Since the obtained temperature range and the maximum density ratio in this model are expanded, and the magnitude of maximum spurious current is only between those of SC and Zhang models, it is believed that this new model has better stability than SC and Zhang models. Consequently, the application scope of this new model is expanded compared with the existing phase transition models. According to the principle of the corresponding states in engineering thermodynamics, the simulations of ammonia and water phase transition process were implemented using this new model with different equations of state. Compared with the experimental data of ammonia and water, the results show that the Peng-Robinson is the best equation of state to describe the phase transition process of ammonia and water. Especially, the simulation results of ammonia with Peng-Robinson equation of state have an excellent agreement with its experimental data. Therefore these simulation results have a significant influence on the real engineering applications. | ZENG JianBang LI LongJian LIAO Quan CUI WenZhi CHEN QingHua PAN LiangMing | 2009 | Chinese Science Bulletin2009，54，24： | 8 |
| 8 | Dermal fibroblast expression of stromal cellderived factor-1 (SDF-1) promotes epidermal keratinocyte proliferation in normal and diseased skin显示文摘Stromal 房间为 overlying 上皮提供关键微型环境。这里,我们调查了 stromal 的表示和功能房间特定的蛋白质, stromal 导出房间的 factor-1 (SDF-1 ) ,在正常人的皮肤并且在 diseased 的纸巾皮肤。Immunohistology 和激光俘获 microdissection (最小公倍数) 联合了揭示的量的即时 RT-PCR 那 SDF-1 组成地并且主要在 vivo 在正常人的皮肤在真皮的 stromal 房间被表示。使我们吃惊的是,一极其, SDF-1 抄写的高水平在 vivo 在正常人的皮肤的真皮被观察,比打字由许多更高的 mRNA 表示水平证实了我骨胶原,在人的皮肤的最丰富、高度表示的蛋白质。SDF-1 也是在包括干癣,基础房间癌(电子消息传输方式) ,和有鳞的房间癌(SCC ) 的许多人的皮混乱的纸巾的 upregulated。为 SDF-1 和 HSP47 (热吃惊蛋白质 47 ) 的两倍 immunostaining,成纤维细胞的一个标记,表明成纤维细胞是在正常和 diseased 皮肤的 stroma-cell-derived SDF-1 的主要来源。机能上地, SDF-1 激活 ERK (extracellular-signal-regulated kinases ) 是的小径和功能刺激表皮的 keratinocyte 增长的 mitogen。在有到皮肤等价物文化的 rhSDF-1 的真皮的成纤维细胞和处理的 SDF-1 的两 overexpression 显著地增加了 keratinocyte 层和表皮的厚度的数字。相反地, keratinocyte 增长上的 SDF-1 的刺激功能被防碍 CXCR4 将近完全消除, SDF-1 的特定的受体,或由在成纤维细胞的 SDF-1 击倒。我们的数据揭示那 SDF-1 极其高级处于生理、病理学的皮肤条件为表皮的 keratinocyte 增长提供关键微型环境。 | Chunji Quan Moon Kvun Cho Yuan Shao Laurel E. Mianecki Eric Liao Daniel Perry Taihao Quan | 2015 | Protein & Cell2015，6，12： | 8 |
| 9 | CuO/zeolite catalyzed oxidation of gaseous toluene under microwave heating显示文摘为甲苯浪费气体的处理加热的催化氧化和微波的联合进程的开发在这个工作被描述。甲苯,典型有毒的不稳定的有机化合物,通过包含催化剂与硅碳化物混合了的支持沸石的铜氧化物(CuO/zeolite ) 的一个固定的床反应房间被氧化(原文如此) ,优秀吸收微波的材料。目标化合物高效地在温度完成了由的高反应在催化剂的表面上被降级加热微波原文如此。一套试验性的参数例如微波力量,让流动和 CuO 等等的装载尺寸通风,分别地被调查。学习证明这些参数在甲苯降级上有批评影响。在最佳的状况下面, 92% 甲苯被这个联合过程移开,相应于 80%90% TOC 移动率。而且,催化剂甚至在八连续 6-h 跑以后是高度稳定的。最后,甲苯的一条假想降级小径基于从红外线的光谱学分析的煤气的层析团光谱和 Fourier 变换获得的试验性的数据被建议。 | Longli BO Jianbo LIAO Yucai ZHANG Xiaohui WAN Quan YANG | 2013 | Frontiers of Environmental Science & Engineering2013，7，3： | 7 |
| 10 | Relationship between single nucleotide polymorphisms in the deoxycytidine kinase gene and chemosensitivity of gemcitabine in six pancreatic cancer cell lines显示文摘在 deoxycytidine kinase (dCK ) 的背景单个核苷酸多型性(SNP ) 基因与 chemosensitivity 被联系到 nucleoside 类似物。2',2'-Difluoro 2'-deoxycytidine (gemcitabine ) 是在胰腺的癌症的治疗的首要的 nucleoside 类似物药。然而,在在到 gemcitabine 的 dCK 基因和 chemosensitivity 的 SNP 之间的协会充分没被建立。因此,现在的学习试图在人的胰腺的癌症房间 lines.Methods 在 dCKgene 和 chemosensitivity 调查在 SNP 之间的关系到 gemcitabine 在 dCK 基因的七 SNP 在六根人的胰腺的癌症房间线被定序。到 gemcitabine 的这六根房间线的 chemosensitivity 与数 Kit-8 ( CCK-8 ) test.Inhibition 率的一个房间在 vitro 被评估被用来表示胰腺的癌症房间线的 chemosensitivity 到 gemcitabine.Results 在 dCKgene 的 A9846G SNP 的遗传型在六个人的胰腺的癌症房间被决定 lines.The 房间线而房间线 AsPC-1 , Mia PaCa2 , SW1990 和 SU86.86 带了 GG 遗传型, BxPC-3 和 T3M4 带了 A9846G SNP 遗传型 AG 。有 AG 遗传型( BxPC-3 和 T3M4 )的房间线与有 GG 遗传型的房间线相比对 gemcitabine 更敏感( AsPC-1 , Mia PaCa2 , SW1990 和 SU86.86 )并且显著地不同的抑制率在带 AG 和 GG 遗传型( P 0.01 ) .Conclusions 的房间线之间被观察在 dCK 基因的 A9846G SNP 的变体在胰腺的癌症房间线与敏感被联系到 gemcitabine 。dCK A9846G SNP 可以充当一个基因标记在胰腺的癌症预言 gemcitabine 的化疗功效。 | SI Shuang LIAO Quan ZHAO Yu-pei HU Ya ZHANG Qiang YOU Li-li | 2011 | Chinese Medical Journal2011，，3： | 5 |
| 11 | Effect of Blumgart anastomosis in reducing the incidence rate of pancreatic fistula after pancreatoduodenectomy显示文摘BACKGROUND Pancreatic fistula is one of the most serious complications after pancreatoduodenectomy for treating any lesions at the pancreatic head. For years, surgeons have tried various methods to reduce its incidence. AIM To investigate and emphasize the clinical outcomes of Blumgart anastomosis compared with traditional anastomosis in reducing postoperative pancreatic fistula. METHODS In this observational study, a retrospective analysis of 291 patients who underwent pancreatoduodenectomy, including Blumgart anastomosis (201 patients) and traditional embedded pancreaticojejunostomy (90 patients), was performed in our hospital. The preoperative and perioperative courses and longterm follow-up status were analyzed to compare the advantages and disadvantages of the two methods. Moreover, 291 patients were then separated by the severity of postoperative pancreatic fistula, and two methods of pancreaticojejunostomy were compared to detect the features of different anastomosis. Six experienced surgeons were involved and all of them were proficient in both surgical techniques.RESULTS The characteristics of the patients in the two groups showed no significant differences, nor the preoperative information and pathological diagnoses. The operative time was significantly shorter in the Blumgart group (343.5 ± 23.0 vs 450.0 ± 40.1 min, P = 0.028), as well as the duration of pancreaticojejunostomy drainage tube placement and postoperative hospital stay (12.7 ± 0.9 d vs 17.4 ± 1.8 d, P = 0.031;and 21.9 ± 1.3 d vs 28.9 ± 1.3 d, P = 0.020, respectively). The overall complications after surgery were much less in the Blumgart group than in the embedded group (11.9% vs 26.7%, P = 0.002). Patients who underwent Blumgart anastomosis would suffer less from severe pancreatic fistula (71.9% vs 50.0%, P = 0.006), and this pancreaticojejunostomy procedure did not have worse influences on long-term complications and life quality. Thus, Blumgart anastomosis is a feasible pancreaticojejunostomy procedure in pancreatoduodenectomy surgery. It is safe in causing less postoperative complications, especially pancreatic fistula, and thus shortens the hospitalization duration. CONCLUSION Surgical method should be a key factor in reducing pancreatic fistula, and Blumgart anastomosis needs further promotion. | Ya-Tong Li Han-Yu Zhang Cheng Xing Cheng Ding Wen-Ming Wu Quan Liao Tai-Ping Zhang Yu-Pei Zhao Meng-Hua Dai | 2019 | World Journal of Gastroenterology2019，25，20： | 5 |
| 12 | Metformin inhibits pancreatic cancer metastasis caused by SMAD4 deficiency and consequent HNF4G upregulation显示文摘Pancreatic ductal adenocarcinoma(PDAC)has poor prognosis due to limited therapeutic options.This study examines the roles of genome-wide association study identified PDAC-associated genes as therapeutic targets.We have identified HNF4G gene whose silencing most effectively repressed PDAC cell invasiveness.HNF4G overexpression is induced by the deficiency of transcriptional factor and tumor suppressor SMAD4.Increased HNF4G are correlated with SMAD4 deficiency in PDAC tumor samples and associated with metastasis and poor survival time in xenograft animal model and in patients with PDAC(log-rank P=0.036;HR=1.60,95%CI=1.03–2.47).We have found that Metformin suppresses HNF4G activity via AMPK-mediated phosphorylation-coupled ubiquitination degradation and inhibits in vitro invasion and in vivo metastasis of PDAC cells with SMAD4 deficiency.Furthermore,Metformin treatment significantly improve clinical outcomes and survival in patients with SMAD4-deficient PDAC(log-rank P=0.022;HR=0.31,95%CI=0.14–0.68)but not in patients with SMAD4-normal PDAC.Pathway analysis shows that HNF4G may act in PDAC through the cell-cell junction pathway.These results indicate that SMAD4 deficiency-induced overexpression of HNF4G plays a critical oncogenic role in PDAC progression and metastasis but may form a druggable target for Metformin treatment. | Chengcheng Wang Taiping Zhang Quan Liao Menghua Dai Junchao Guo Xinyu Yang Wen Tan Dongxin Lin Chen Wu Yupei Zhao | 2021 | Protein & Cell2021，12，2： | 5 |
| 13 | Associations between gene polymorphisms of thymidylate synthase with its protein expression and chemosensitivity to 5-fluorouracil in pancreatic carcinoma cells显示文摘背景 Thymidylate synthase ( TS )是关键规章的酶因为 de novo DNA synthesis.TS 活动也是对有 fluoropyrimidine prodrugs 的化疗的反应的一个重要决定因素,并且它的表示可以被基因 polymorphisms.In 影响这研究,我们调查了在 TS 基因和它的蛋白质的多型性之间的协会在胰腺的癌症 cells.Methods 遗传型的5氟尿嘧啶( 5-FU )的功效上的表示,和含意基于 28-bp TS 双人脚踏车重复 | ZHANG Qiang ZHAO Yu-pei LIAO Quan HU Ya XU Qiang ZHOU Li SHU Hong | 2011 | Chinese Medical Journal2011，，2： | 4 |
| 14 | Recent studies of 5-fluorouracil resistance in pancreatic cancer显示文摘Resistance to 5-fluorouracil(5-FU), an important anticancer drug, is a serious challenge in the treatment of pancreatic cancer. Equilibrative nucleoside transporter 1 and multidrug-resistance protein(MRP) 5 and MRP8, rather than P-glycoprotein, play important roles in 5-FU transport. Thymidylate synthase, dihydropyrimidine dehydrogenase, methylenetetrahydrofolate reductase and thymidine phosphorylase are four key enzymes involved in 5-FU metabolism. Other metabolic enzymes, including uridine monophosphate synthetase, also contribute to chemoresistance. Intracellular signaling pathways are an integrated network, and nuclear factor kappa-light-chain-enhancer of activated B cells, AKT and extracellular signal-regulated kinases are signaling pathways that are particularly relevant to 5-FU resistance. In addition, recent reports indicate that STAT-3 is a crucial survival protein. Proteomic assays provide a powerful tool for identifying target proteins and understanding the role of micro RNAs and stromal factors to facilitate the development of strategies to combat 5-FU resistance. | Wei-Bin Wang Yu Yang Yu-Pei Zhao Tai-Ping Zhang Quan Liao Hong Shu | 2014 | World Journal of Gastroenterology2014，20，42： | 3 |
| 15 | Heat Transfer Effectiveness of Three-Fluid Separated Heat Pipe Exchanger显示文摘A heat transfer model for three-fluid separated heat pipe exchanger was analyzed,and the temperature transfer matrix for general three-fluid separated heat exchanger working in parallel-flow or counter-flow mode was obtained.It was found that the forms of temperature transfer matrix are similar for heat pipe rows with equal or different heat transfer surface area.Furthermore,by using the temperature transfer matrix of the heat pipe exchanger,the relationship between heat transfer effectiveness θ 1,θ 2 and M,NTU,U,Δt i were derived for the exchanger operating in parallel-flow or counter-flow mode,and a simple special example was adopted to demonstrate the correctness of these relationships. | Chengming Shi Yang Wang Ying Yang Quan Liao | 2011 | Journal of Thermal Science2011，20，1： | 3 |
| 16 | Mitotic motor CENP-E cooperates with PRC1 in temporal control of central spindle assembly显示文摘Error-free cell division depends on the accurate assembly of the spindle midzone from dynamic spindle microtubules to ensure chromatid segregation during metaphase-anaphase transition.However,the mechanism underlying the key transition from the mitotic spindle to central spindle before anaphase onset remains elusive.Given the prevalence of chromosome instability phenotype in gastric tumorigenesis,we developed a strategy to model context-dependent cell division using a combination of light sheet microscope and 3D gastric organoids.Light sheet microscopic image analyses of 3D organoids showed that CENP-E inhibited cells undergoing aberrant metaphase-anaphase transition and exhibiting chromosome segregation errors during mitosis.Highresolution real-time imaging analyses of 2D cell culture revealed that CENP-E inhibited cells undergoing central spindle splitting and chromosome instability phenotype.Using biotinylated syntelin as an affinity matrix,we found that CENP-E forms a complex with PRC1 in mitotic cells.Chemical inhibition of CENP-E in metaphase by syntelin prevented accurate central spindle assembly by perturbing temporal assembly of PRC1 to the midzone.Thus,CENP-E-mediated PRC1 assembly to the central spindle constitutes a temporal switch to organize dynamic kinetochore microtubules into stable midzone arrays.These findings reveal a previously uncharacterized role of CENP-E in temporal control of central spindle assembly.Since CENP-E is absent from yeast,we reasoned that metazoans evolved an elaborate central spindle organization machinery to ensure accurate sister chromatid segregation during anaphase and cytokinesis. | Xu Liu Leilei Xu Junying Li Phil Y.Yao Wanjuan Wang Hazrat Ismail Haowei Wang Bryce Liao Zhihong Yang Tarsha Ward Ke Ruan Jianchun Zhang Quan Wu Ping He Xia Ding Dongmei Wang Chuanhai Fu Zhen Dou Feng Yan Wenwen Wang Xing Liu Xuebiao Yao | 2020 | Journal of Molecular Cell Biology2020，12，8： | 3 |
| 17 | Risk factors for postoperative pancreatic fistula in patients with insulinomas:analysis of 292 consecutive cases显示文摘BACKGROUND:Pancreatic fistula (PF) remains the most challenging complication in pancreatic surgery,yet few published studies have focused on the risk factors for postoperative PF in patients undergoing surgery for insulinomas.METHODS:From January 1990 to February 2010,a total of 292 patients with insulinomas underwent surgery at Peking Union Medical College Hospital.Demographic data,intraoperative procedures,and postoperative data were collected.Particular attention was paid to variables associated with PF as defined by the International Study Group of Pancreatic Fistula (ISGPF).Univariate and multivariate analyses were used to identify possible risk factors for PF.RESULTS:PF was found in 132 (45.2%) patients,of whom 90 were classified into ISGPF grade A,33 grade B,and 9 grade C.Multivariate analysis showed that male patients (OR=2.56;P=0.007) and operative time >180minutes (OR=3.756;P<0.0001) were independent risk factors for clinical PF.Pancreatic resection with stapler was a protective factor for both total PF (OR=0.022;P=0.010) and clinical PF (OR=0.097;P=0.007).CONCLUSIONS:Male gender and operative time >180minutes were independent risk factors for clinical PF,while pancreatic resection with a stapler was a protective factor.Whether body mass index (BMI) and other variables during operation are risk factors of PF needs further study. | Zhao, Yu-Pei Zhan, Han-Xiang Cong, Lin Zhang, Tai-Ping Liao, Quan Dai, Meng-Hua Cai, Li-Xing Zhu, Yu | 2012 | Hepatobiliary & Pancreatic Diseases International2012，11，1： | 3 |
| 18 | Gastroesophageal Varices (Bleeding) and Splenomegaly: The Initial Manifestations of Some Pancreatic Body and Tail Carcinoma显示文摘INTRODUCTION The most common symptoms of the pancreatic body and tail carcinoma are epigastric pain,asthenia and back pain.However,these symptoms are nonspecific,which as a consequence leads to late diagnosis as well as a low resection rate between 10% and 30%,with a 5 years overall survival rate below 10%.[1] That how to increase early diagnosis of the pancreatic body and tail carcinoma has been a difficult problem since then.Sometimes the pancreatic body and tail carcinoma can induce splenic vein occlusion,leading to pancreatic sinistral portal hypertension (PSPH).PSPH is defined as a pathological condition of splanchnic venous hypertension localized within the left-sided gastrosplenic region in pancreatic diseases. | Xiao-Bin Li Luo Zhao Quan Liao Qiang Xu Tai-Ping Zhang Lin Cong Bing Mu | 2015 | Chinese Medical Journal2015，，4： | 3 |
| 19 | “Pincer movement”:Reversing cisplatin resistance based on simultaneous glutathione depletion and glutathione S-transferases inhibition by redox-responsive degradable organosilica hybrid nanoparticles显示文摘The therapeutic efficacy of cisplatin has been restricted by drug resistance of cancers.Intracellular glutathione(GSH)detoxification of cisplatin under the catalysis of glutathione S-transferases(GST)plays important roles in the development of cisplatin resistance.Herein,a strategy of“pincer movement”based on simultaneous GSH depletion and GST inhibition is proposed to enhance cisplatin-based chemotherapy.Specifically,a redox-responsive nanomedicine based on disulfide-bridged degradable organosilica hybrid nanoparticles is developed and loaded with cisplatin and ethacrynic acid(EA),a GST inhibitor.Responding to high level of intracellular GSH,the hybrid nanoparticles can be gradually degraded due to the break of disulfide bonds,which further promotes drug release.Meanwhile,the disulfide-mediated GSH depletion and EA-induced GST inhibition cooperatively prevent cellular detoxification of cisplatin and reverse drug resistance.Moreover,the nanomedicine is integrated into microneedles for intralesional drug delivery against cisplatin-resistant melanoma.The in vivo results show that the nanomedicine-loaded microneedles can achieve significant GSH depletion,GST inhibition,and consequent tumor growth suppression.Overall,this research provides a promising strategy for the construction of new-type nanomedicines to overcome cisplatin resistance,which extends the biomedical application of organosilica hybrid nanomaterials and enables more efficient chemotherapy against drug-resistant cancers. | Boyi Niu Yixian Zhou Kaixin Liao Ting Wen Sixian Lao Guilan Quan Xin Pan Chuanbin Wu | 2022 | Acta Pharmaceutica Sinica B2022，12，4： | 3 |
| 20 | Research on Propagation Characteristics of XLPE Cable Electrical Trees in different Electrode System显示文摘The electrical tree discharge channel will be formed at concentrate spot of electric field in solid insulation dielectric,in order to study the difference of electrical tree under different electrical field,the short-cable electrode system with actual XLPE cable was designed,experiments were performed under 12 kV,15 kV,18 kV,21 kV compare to the needle-plate electrode system.Experiment results show that the electrical tree of short-cable electrode system have the same growth trend with the needle-plate electrode system in the growing characteristic,the dense of electrical tree increase with the increase of voltage level,electrical tree of short-cable electrode system growth is slower than the needle-plate electrode system at the same voltage;To get the same shape of electrical tree,the voltage of short-cable electrode system must be higher than needle-plate electrode system,the results show that the semiconductor layer and the copper shield layer outside of XLPE cable have very important affection on the electrical trees degradation. | ZHOU Quan YE Di LIAO Rui-jin LIU Jia-bin REN Hai-jun CHEN Shi-jun | 2008 | 高电压技术2008，34，12： | 3 |
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