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| 1 | Cellular membrane cholesterol is required for porcine reproductive and respiratory syndrome virus entry and release in MARC-145 cells显示文摘Cholesterol represents one of the key constituents of small,dynamic,sterol-and sphingolipid-enriched domains on the plasma membrane.It has been reported that many viruses depend on plasma membrane cholesterol for efficient infection.In this study,the role of the plasma membrane cholesterol in porcine reproductive and respiratory syndrome virus(PRRSV) infection of MARC-145 cells was investigated.Pretreatment of MARC-145 cells with methyl-β-cyclodextrin(MβCD),a drug used to deplete cholesterol from cellular membrane,significantly reduced PRRSV infection in a dose-dependent manner.This inhibition was partially reversed by supplementing exogenous cholesterol following MβCD treatment,suggesting that the inhibition of PRRSV infection was specifically mediated by removal of cellular cholesterol.Further detailed studies showed that depletion of cellular membrane cholesterol significantly inhibited virus entry,especially virus attachment and release.These results indicate that the presence of cholesterol in the cellular membrane is a key component of PRRSV infection. | SUN Ying XIAO ShaoBo WANG Dang LUO Rui LI Bin CHEN HuanChun FANG LiuRong | 2011 | Science China(Life Sciences)2011，54，11： | 3 |
| 2 | Bioinspired synthesis of CVD graphene flakes and graphene-supported molybdenum sulfide catalysts for hydrogen evolution reaction显示文摘化学蒸汽免职是为在平面底层上种大区域的高质量的 graphene 电影的大多数有希望途径。在侧面的生长以外,合成三维(3D ) graphene 最近也在金属泡沫和绝缘的 nanoparticles 上被表明了为在电气化学的电极探索他们的应用。然而,存在途径也需要丰富的开始预制,或为 graphene 生长构造多孔的框架底层。此处,我们报导一直接,为用化学蒸汽免职(CVD ) 在乌贼骨底层上种大数量的 graphene 薄片的 bioinspired 策略方法。从生长底层的分开的 graphene 薄片是高度水晶的,超过并且层厚度可控制传统化学上,有很少表面的 exfoliated graphene 组织。由于他们继承导出 biomineral 的形态学, 3D graphene 微观结构显示出高度暴露并且弯的表面,它能装载更多的瞬间 x (x 2 ) 催化剂比对高度有效的氢产生的另外的平面支持。简短, bioinspired 途径被期望为 graphene 生产完成在质量和数量之间的合理平衡,因此推进它在精力的宽应用存储和变换设备。 | Ke Chen Cong Li Zhaolong Chen Liurong Shi Sathish Reddy Huan Meng Qingqing Ji Yanfeng Zhang Zhongfan Liu | 2016 | Nano Research2016，9，1： | 2 |
| 3 | Porcine reproductive and respiratory syndrome virus (PRRSV) suppresses interferon-β production by interfering with the RIG-I signaling pathway显示文摘 | Rui Luo Shaobo Xiao Yunbo Jiang Hui Jin Dang Wang Manli Liu Huanchun Chen Liurong Fang | 2008 | Molecular Immunology2008，，10： | 2 |
| 4 | CRISPR-based genome editing technology and its applications in oil crops显示文摘Oil crops,mainly comprised of soybean,rapeseed,groundnut,sunflower and etc.,have provided substantial edible oil and other tremendous nutrients for human beings,as well as valuable biofuels for associated industries.The genetic improvement of significant oil crops and/or domesticating novel high-yielding oil crops are in urgent need to cope with the ever-increasing demand for various oil crop products.CRISPR(Clustered Regularly Interspaced Short Palindromic Repeats)-based genome editing technology,born a few years ago,edits stretches of DNA in a targeted and RNA-dependent fashion.The Characteristics of targeted mutagenesis and easy manipulation owned by the technology make it have been applied to many plants and exhibited great potential in the genetic improvement of many important oil crops.In the face of growing need for oil crop products and the rapid developments in CRISPR-based genome editing technology,a critical review regarding the technology and its application in oil crops is badly required to provide references for the better use of this technology to modify the oil crops for higher yield.In this review paper,we briefly described the CRISPR-based genome editing technology and summarized its applications and future prospects in oil crops. | Jianjie He Kai Zhang Mi Tang Weixian Zhou Liurong Chen Zhaobo Chen Maoteng Li | 2021 | Oil Crop Science2021，6，3： | 1 |
| 5 | Antiviral activity of type I and type III interferons against porcine reproductive and respiratory syndrome virus (PRRSV)显示文摘 | Rui Luo Liurong Fang Hui Jin Yunbo Jiang Dang Wang Huanchun Chen Shaobo Xiao | 2011 | Antiviral Research2011，，2： | 1 |
| 6 | Foot-and-mouth disease virus (FMDV) leader proteinase negatively regulates the porcine interferon-λ1 pathway显示文摘 | Dang Wang Liurong Fang Lizhi Liu Huijuan Zhong Quangang Chen Rui Luo Xiangtao Liu Zhongming Zhang Huanchun Chen Shaobo Xiao | 2011 | Molecular Immunology2011，，1： | 1 |
| 7 | Porcine Reproductive and Respiratory Syndrome Virus Induces IL-1 β Production Depending on TLR4/MyD88 Pathway and NLRP3 Inflammasome in Primary Porcine Alveolar Macrophages显示文摘 | Jing Bi Shuang Song Liurong Fang Dang Wang Huiyuan Jing Li Gao Yidong Cai Rui Luo Huanchun Chen Shaobo Xiao Vera L. Petricevich | 2014 | Mediators of Inflammation2014，，： | 1 |
| 8 | Molecular cloning, expression and antiviral activity of porcine interleukin-29 (poIL-29)显示文摘 | Dang Wang Liurong Fang Fuwei Zhao Rui Luo Huanchun Chen Shaobo Xiao | 2010 | Developmental and Comparative Immunology2010，，3： | 1 |
| 9 | Induction of autophagy enhances porcine reproductive and respiratory syndrome virus replication显示文摘 | Quangang Chen Liurong Fang Dang Wang Shaohua Wang Ping Li Mao Li Rui Luo Huanchun Chen Shaobo Xiao | 2011 | Virus Research2011，，2： | 1 |
| 10 | Hypodermin A, a potential agent for prevention of allogeneic acute rejection显示文摘 | Quangang Chen Renjin Chen Jing Liu Honghua Yuan Peng Liu Ankang Hu Lianlian Wu Liurong Fang Shaobo Xiao Xiaorong Zhu | 2015 | Transplant Immunology2015，，3： | 1 |
| 11 | Porcine reproductive and respiratory syndrome virus (PRRSV) infection activates chemokine RANTES in MARC-145 cells显示文摘 | Yanwei Wang Rui Luo Liurong Fang Dang Wang Jing Bi Huanchun Chen Shaobo Xiao | 2010 | Molecular Immunology2010，，4： | 1 |
| 12 | Porcine reproductive and respiratory syndrome virus infection activates NOD2–RIP2 signal pathway in MARC-145 cells显示文摘 | Huiyuan Jing Liurong Fang Dang Wang Zhen Ding Rui Luo Huanchun Chen Shaobo Xiao | 2014 | Virology2014，，： | 1 |
| 13 | Construction and characterization of a live, attenuated apxIICA inactivation mutant of Actinobacillus pleuropneumoniae lacking a drug resistance marker显示文摘 | Weicheng Bei Qigai He Lin Yan Liurong Fang Yadi Tan Shaobo Xiao Rui Zhou Meilin Jin Aizhen Guo Jianqiang Lv Hongliang Huang Huanchun Chen | 2004 | FEMS Microbiology Letters2004，，1： | 1 |
| 14 | Induction of autophagy enhances porcine reproductive and respiratory syndrome virus replication显示文摘 | Quangang Chen Liurong Fang Dang Wang Shaohua Wang Ping Li Mao Li Rui Luo Huanchun Chen Shaobo Xiao | 2011 | Virus Research2011，，2： | 1 |
| 15 | Immunogenicity of porcine circovirus type 2 capsid protein targeting to different subcellular compartments显示文摘 | Huiying Fan Shaobo Xiao Tiezhu Tong Shengpin Wang Lilan Xie Yunbo Jiang Huanchun Chen Liurong Fang | 2007 | Molecular Immunology2007，，： | 1 |
| 16 | Construction and immunogenicity of recombinant pseudorabies virus expressing the modified GP5m protein of porcine reproduction and respiratory syndrome virus显示文摘Pseudorabies virus(PRV),an alpha-herpesvirus,has been developed as a live viral vector for animal vaccines.However,the PRV recombinant virus TK^(-)/gE^(-)/GP5^(+)expressing GP5 of porcine reproductive and respiratory syn-drome virus(PRRSV),based on the PRV genetically depleted vaccine strain TK^(-)/gE^(-)/LacZ^(+),scarcely stimulated the vaccinated animals to produce neutralizing antibodies against PRRSV.To develop a booster-specific immune response of such PRV recombinants,the ORF5m gene(the modified ORF5 gene having better immune responses)was substituted for the ORF5 gene and introduced into PRV TK^(-)/gE^(-)/LacZ^(+),resulting in a PRV recombinant named TK^(-)/gE^(-)/GP5m^(+),which expressed the modified GP5m protein.The recombinant virus was confirmed using PCR,Southern blotting and Western blotting.TK^(-)/gE^(-)/GP5m^(+)and TK^(-)/gE^(-)/GP5^(+)expressing the authentic GP5 protein were inoculated into Balb/c mice to evaluate their immune responses.The results indicated that the protecting neutralization antibodies(the 3/6 vaccinated mice obtained 1:16)and cell immune responses induced by TK^(-)/gE^(-)/GP5m^(+)against PRRSV were higher than that induced by TK^(-)/gE^(-)/GP5^(+).Thus,the development of the new PRV recombinant expressing the modified GP5m protein as a candidate vaccine established the basis for the study of bivalent genetic engineering vaccines against PRRSV and PRV. | JIANG Yunbo FANG Liurong XIAO Shaobo ZHANG Hui CHEN Huanchun | 2007 | Frontiers in Biology2007，2，1： | 0 |
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