|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Research advances in the detection of miRNA显示文摘MicroRNAs (miRNAs) are a family of endogenous, small (approximately 22 nucleotides in length), noncoding, functional RNAs. With the development of molecular biology, the research of miRNA biological function has attracted significant interest, as abnormal miRNA expression is identified to contribute to serious human diseases such as cancers. Traditional methods for miRNA detection do not meet current demands. In particular, nanomaterial-based methods, nucleic acid amplification-based methods such as rolling circle amplification (RCA), loop-mediated isothermal amplification (LAMP), strand-displacement amplification (SDA) and some enzyme-free amplifications have been employed widely for the highly sensitive detection of miRNA. MiRNA functional research and clinical diagnostics have been accelerated by these new techniques. Herein, we summarize and discuss the recent progress in the development of miRNA detection methods and new applications. This review will provide guidelines for the development of follow-up miRNA detection methods with high sensitivity and specificity, and applicability to disease diagnosis and therapy. | Jiawei Ye Mingcheng Xu Xueke Tian Sheng Cai Su Zeng | 2019 | Journal of Pharmaceutical Analysis2019，9，4： | 15 |
| 2 | Adaptive Walking of a Quadrupedal Robot Based on Layered Biological Reflexes显示文摘A multiple-legged robot is traditionally controlled by using its dynamic model.But the dynamic-model-based approach fails to acquire satisfactory performances when the robot faces rough terrains and unknown environments.Referring animals' neural control mechanisms,a control model is built for a quadruped robot walking adaptively.The basic rhythmic motion of the robot is controlled by a well-designed rhythmic motion controller(RMC) comprising a central pattern generator(CPG) for hip joints and a rhythmic coupler(RC) for knee joints.CPG and RC have relationships of motion-mapping and rhythmic couple.Multiple sensory-motor models,abstracted from the neural reflexes of a cat,are employed.These reflex models are organized and thus interact with the CPG in three layers,to meet different requirements of complexity and response time to the tasks.On the basis of the RMC and layered biological reflexes,a quadruped robot is constructed,which can clear obstacles and walk uphill and downhill autonomously,and make a turn voluntarily in uncertain environments,interacting with the environment in a way similar to that of an animal.The paper provides a biologically inspired architecture,with which a robot can walk adaptively in uncertain environments in a simple and effective way,and achieve better performances. | ZHANG Xiuli E Mingcheng ZENG Xiangyu ZHENG Haojun | 2012 | Chinese Journal of Mechanical Engineering2012，25，4： | 10 |
| 3 | Allele-defined genome reveals biallelic differentiation during cassava evolution显示文摘Dear Editor,Generation of heterozygous genomes by hybridization between or within species can help maintain plant diversity and serve as a potential source of new species(Baek et al.,2018).Moreover,genomic heterozygosity is associated with genomic coadaptation,developmental stability,and heterosis.Accurate definition of alleles in haplotypes is necessary to precisely characterize allelic variation controlling agriculturally important traits(Shi et al.,2019).Currently,most released genomes have mosaic assembly of haplotypes due to random selection or collapse of alleles during genome assembly(Shi et al.,2019),which masked allelic variation and functional differentiation of divergent alleles in heterozygous species. | Wei Hu Changmian Ji Haitao shi Zhe Liang Zehong Ding Jianqiu Ye Wenjun Ou Gang Zhou Weiwei Tie Yan Yan Jinghao Yang Xiaoying Yang Yunxie Wei Zhiqiang Jin Jianghui Xie Ming Peng Wenquan Wang Anping Guo Biyu Xu Jianchun Guo Songbi Chen Liming Ma Mingcheng Wang Yang Zhou Xiaolong Li Ruoxi Li Siyi Guo Xinhui Xiao Zhongqing Wan Feifei An Jie Zhang Qingyun Leng Yin Li Ray Ming Kaimian Li | 2021 | Molecular Plant2021，14，6： | 8 |
| 4 | Synthesis and properties of p(NIPA-co-NVP)/clay hydrogels by radiation polymerization显示文摘In this study,hydrogels of P(NIPA-co-NVP)/clay were synthesized byγ-ray radiation.Different thermo-sensitive hydrogels were made under different experimental conditions such as dose,monomer ratio,and con- tent of clay.X-ray diffraction shows that the layer distance between Na-clays is changed from 1.6 to 2.7 nm because Na-clay pieces can be intercalated or exfoliated by HTMAB,and that between P(NIPA-co-NVP)/clay pieces is 3.4 nm. The swelling property tests show that the LCST of P(NIPA-co-NVP) is higher than PNIPA.With the increase of NVP content,LCST is higher.As the ratio of NIPA/NVP is 95/5,hydrogel shows the best swelling property and LCST is 37℃.LCST of P(NIPA-co-NVP)/clay hydrogel is not changed,but the strength and swelling properties are better. | SONG Hongyan YANG Mingcheng ZHU Chengshen HE Suqin GAO Ya | 2007 | Nuclear Science and Techniques2007，18，1： | 4 |
| 5 | Epicardial adipose tissue: the accomplice implicated in the genesis and maintenance of atrial fibrillation显示文摘 | Fang Mingcheng Chen Yangxin Wang Jingfeng | 2014 | Chinese Medical Journal2014，，14： | 4 |
| 6 | A study on water absorption in freestanding polyurethane films filled with nano-TiO_2 pigments by capacitance measurements显示文摘The electrochemical impedance spectroscopy (EIS) was used to evaluate the water transport and dielectric properties of polyurethane films filled with nano-TiO2 at different pigment/base (P/B) values in 0.5 mol/L NaCl solutions. EIS results were compared with gravimetric measurements on the freestanding films. The amount of water absorption showed great discrepancy between the two methods. The diffusion coeffcient in the polyurethane film with P/B=30% was the smallest among those filled with nano-TiO2 pigments. The dielectric constant ε of the polyurethane varnish film obtained from the initial capacitance was in the range of typical values of polymers. SEM was used to measure the distribution of nano-TiO2 particles in the polyurethane films. | Mingcheng SUN Fuchun LIU Hongwei SHI Enhou HAN | 2009 | Acta Metallurgica Sinica(English Letters)2009，22，1： | 3 |
| 7 | Swelling and thermal properties of porous PNIPAM/PEG hydrogels prepared by radiation polymerization显示文摘In this study,a series of porous intelligent hydrogels were synthesized by radiation exhibiting the lower critical solution temperature(LCST) and fast response involving a combination of A’-isopropyl acrylamideas monomer, polyethylene glycol(PEG) as pore-forming agent and N,N-methylene-bis-acrylamide as crosslinking agent.The hydrogels were analyzed by Fourier transform infrared spectroscopy,and the influence of radiation doses on their swelling and thermal behaviors were studied.Their surface morphologies were examined by scanning electron microscopy.The results showed that PEG molecules only acted as pore-forming agent in the cross-linked polymerization.Their swelling ratios reduced with increasing radiation doses.The LCST was around 37℃,and varied little with the radiation doses.The frozen water content of PNIPAM/PEG6000 hydrogel reduced with increasing the radiation dose,and was greater than that of PN1PAM hydrogel at 15 kGy.Hydrogel macropores were prepared by PEG agent,and the hydrogels without PEG had a dense surface.The porous hydrogels are expected to be applied in the field of artificial intelligence material. | LI Zhihui LIU Wentao LI Zhongyuan YANG Mingcheng GAO Xujing CUI Haitao HE Suqin ZHU Chengshen | 2013 | Nuclear Science and Techniques2013，24，2： | 3 |
| 8 | ADSORPTION DYNAMICS OF MACROPOROUS POLYMERIC ADSORBENT Ⅱ. The Studies on the Film Diffusion Mass-Transfer Process显示文摘The film diffusion mass-transfer process of adsorption of phenol on macroporous polystyrene resin was investigated in detail In order to revise the Boyd film diffusion kinetics equation, the out-surface structure of the macroporous resin and that of gel-type ion-exchange resin was compared and the new film diffusion equation was also suggested. These results showed that the film diffusion was influenced by porosity of the macroporous resin greatly, which differed from the film diffusion behavior of ion-exchange resin obviously. | WANG Chunhong, XU Mingcheng, SHI Zuoqing, FAN Yunge, FI Chengiie (Institute of Polymer Chemistry, Nankai University, Tianjin, 300071) | 2000 | Chinese Journal of Reactive Polymers2000，9，1： | 3 |
| 9 | Effect of Different Sowing Dates in South Henan 's Rice-growing Areas on the Growth and Yield of Ratoon Rice显示文摘In order to determine the optimal sowing date of ratoon rice in South Henan's rice-growing areas,this paper performs a comparative analysis of rice growth process,seedling quality and yield of first season rice and ratoon rice under different sowing date treatments. The results show that under climatic conditions( 2014),by using dry seedling cultivation in a small plastic shed,the growth of seedling sown on February21 was affected,while the sowing treatments from March 1 to April 11 can breed normal seedlings,and in this period,the maturity period of first season rice was delayed with prolonged sowing date,and ratoon rice yield declined with prolonged sowing date( total production of rice sown on 11 March reaching a peak). Thus,it is considered that the optimal sowing date of ratoon rice in South Henan's rice-growing areas is mid-March. | Xiangchen LIU Daqing FENG Guilong YU Haiying ZHAO Li QIAO Yanting LI Xuejun FAN Mingcheng LIU Qiuju ZHANG | 2016 | Asian Agricultural Research2016，8，2： | 3 |
| 10 | Light-driven single-cell rotational adhesion frequency assay显示文摘The interaction between cell surface receptors and extracellular ligands is highly related to many physiological processes in living systems.Many techniques have been developed to measure the ligand-receptor binding kinetics at the single-cell level.However,few techniques can measure the physiologically relevant shear binding affinity over a single cell in the clinical environment.Here,we develop a new optical technique,termed single-cell rotational adhesion frequency assay(scRAFA),that mimics in vivo cell adhesion to achieve label-free determination of both homogeneous and heterogeneous binding kinetics of targeted cells at the subcellular level.Moreover,the scRAFA is also applicable to analyze the binding affinities on a single cell in native human biofluids.With its superior performance and general applicability,scRAFA is expected to find applications in study of the spatial organization of cell surface receptors and diagnosis of infectious diseases. | Yaoran Liu Hongru Ding Jingang Li Xin Lou Mingcheng Yang Yuebing Zheng | 2022 | eLight2022，2，1： | 2 |
| 11 | ADSORPTION DYNAMICS OF MACROPOROUS POLYMERIC ADSORBENT I.The Studies on the Particle Diffusion Mass-Transfer Process显示文摘The adsorption dynamics for phenol in aqueous solution of the adsorbent based on polystyrene was studied. In order to distinguish with the Boyd quasi-homogeneous model of the inner structure of ion-exchanger, the particle diffusion model including surface diffusion model and pore diffusion model was suggested which is suitable to the macroporous adsorbent. The diffusiondetermination step of the adsorption process was established and the effective diffusion coefficient was also determined. The influence of surface diffusion and pore diffusion on the particle diffusion rate was investigated qualitatively. All of these were very important to improve the structure of the macroporous adsorbent in order to improve the mass-transfer rate. | WANG Chunhong ,XU Mingcheng, SHI Zuoqing, FAN Yunge ,JI Chengjie (Institute of Polymer Chemistry, Nankai University ,Tianjin, 300071) | 2000 | Chinese Journal of Reactive Polymers2000，9，1： | 2 |
| 12 | A New Method for Quantitative Simulating Hydrocarbo显示文摘We should understand that the content of chlo-roform bitumen“A”from source rocks at any depth(or period)is not the content of residuary oil,but it is the oil content of source rocks in a condition of dynamic equilibrium of generation and expulsion in an 1nstant.Sc can be measured by laboratory experiment and determined from the curve of S-A'versus depth for the same source rock.(Fig.l) | Mingcheng Li(China University of Geosciences,10083,Beijing,China) | 1994 | China Oil & Gas1994，1，4： | 2 |
| 13 | Preparation of ZrB_(2)-MoSi_(2) high oxygen resistant coating using nonequilibrium state powders by self-propagating high-temperature synthesis显示文摘To achieve high oxygen blocking structure of the ZrB_(2)-MoSi_(2) coating applied on carbon structural material,ZrB_(2)-MoSi_(2) coating was prepared by spark plasma sintering(SPS)method utilizing ZrB_(2)-MoSi_(2) composite powders synthesized by self-propagating high-temperature synthesis(SHS)technique as raw materials.The oxygen blocking mechanism of the ZrB_(2)-MoSi_(2) coatings at 1973 K was investigated.Compared with commercial powders,the coatings prepared by SHS powders exhibited superior density and inferior oxidation activity,which significantly heightened the structural oxygen blocking ability of the coatings in the active oxidation stage,thus characterizing higher oxidation protection efficiency.The rise of MoSi_(2) content facilitated the dispersion of transition metal oxide nanocrystals(5-20 nm)in the SiO_(2) glass layer and conduced to the increasing viscosity,thus strengthening the inerting impact of the compound glass layer in the inert oxidation stage.Nevertheless,the ZrB_(2)-40 vol% MoSi_(2) coating sample prepared by SHS powders presented the lowest oxygen permeability of 0.3% and carbon loss rate of 0.29×10^(6)g·cm^(-2)·s^(-1).Owing to the gradient oxygen partial pressure inside the coatings,the Si-depleted layer was developed under the compound glass layer,which brought about acute oxygen erosion. | Menglin ZHANG Xuanru REN Mingcheng ZHANG Songsong WANG Li WANG Qingqing YANG Hongao CHU Peizhong FENG | 2021 | Journal of Advanced Ceramics2021，10，5： | 2 |
| 14 | Transferability of Microsatellite Markers from Brachypodium distachyon to Miscanthus sinensis,a Potential Biomass Crop显示文摘Miscanthus sinensis has high biomass yield and contributed two of the three genomes in M.x giganteus,a bioenergy crop widely studied in Europe and North America,and thus is a potential biomass crop and an important germplasm for Miscanthus breeding.Molecular markers are essential for germplasm evaluation,genetic analyses and new cultivar development in M.sinensis.In the present study,we reported transferability of simple sequence repeat (SSR) markers from Brachypodium distachyon to M.sinensis.A set of 57 SSR markers evenly distributed across the B.distachyon genome were deliberately designed.Out of these B.distachyon SSR markers,86.0% are transferable to M.sinensis.The SSR loci amplified in M.sinensis were validated by re-sequencing the amplicons.The polymorphism information content (PIC) of the transferable SSR markers varied from 0.073 to 0.375 with a mean of 0.263,assessed based on 21 M.sinensis genotypes.Phylogenetic tree based on 162 alleles detected by 49 SSR markers could unambiguously distinguish B.distachyon from M.sinensis,and cluster 21 M.sinensis genotypes into three groups that are basically in coincidence with their geographical distribution and ecotype classifications.The markers developed by the comparative genomic approach could be useful for germplasm evaluation,genetic analysis,and marker-assisted breeding in Miscanthus. | Hua Zhao Jiangyan Yu Frank M. You Mingcheng Luo Junhua Peng | 2011 | Journal of Integrative Plant Biology2011，53，3： | 2 |
| 15 | Preparation and Characterization of Fe3O4 Magnetic Nano-particles by ^(60)Co γ-ray Irradiation显示文摘由使用一个新方法, 60Co gamma光线照耀, Fe3O4 magneticnano粒子成功地在这些nano粒子的周围的 pressure.Thestructure ,形态学和磁性下面在房间温度被综合被 X-raydiffraction ( XRD )描绘,传播电子显微镜( TEM )和颤动的 samplemagnetometer ( VSM ), respectively.The 放射形成机制也是吸收剂量能极大地影响的 discussed.The 结果 showthat 结构。 | Mingcheng YANG Hongyan SONG Chengshen ZHU Suqin HE Ya GAO | 2007 | Journal of Materials Science & Technology2007，23，2： | 2 |
| 16 | Radiation pre-vulcanization effect on properties of the truck tyre's transition layer and the truck tyre显示文摘In this paper,the natural rubber is chosen as the main constituents for the transition layer of all-steel load radial tyre,which is pre-vulcanized by 500-keV E-beam irradiation of up to 60 kGy.The results show that the green strength of transitional layer increases with the dose,reaching four times as much as the control(without irradiation) at 60 kGy.The final mechanical properties do not differ significantly from those of the control except that the aging and fatigue performance increased.However,thickness of the natural rubber transitional layer for an average single tyre can be reduced by 1 mm(or 1.5 kg) without obvious adverse effect on tyre performance. | YANG Mingcheng ZHANG Benshang ZHU Jun LI Zhaopeng LI Kunhao GUO Dongquan ZHANG Hongna | 2013 | Nuclear Science and Techniques2013，24，2： | 2 |
| 17 | Oxidation of 316 stainless steel in supercritical water显示文摘 | Mingcheng Sun Xinqiang Wu Zhaoen Zhang En-Hou Han | 2009 | Corrosion Science2009，，5： | 2 |
| 18 | SLC1A1-mediated cellular and mitochondrial influx of R-2-hydroxyglutarate in vascular endothelial cells promotes tumor angiogenesis in IDH1-mutant solid tumors显示文摘Muta nt isocitrate dehydrog en ase 1(mlDH1)drives tumorigenesis via produci ng on cometabolite R-2-hydroxyglutarate(R-2-HG)across various tumor types.However,mlDHl in hibitors appear only effective in hematological tumors.The therapeutic ben efit in solid tumors remains elusive,likely due to the complex tumor microenvironment.In this study,we discover that R-2-HG produced by IDH1-mutant tumor cells is preferentially imported into vascular endothelial cells and remodels mitochondrial respiration to promote tumor angiogenesis,conferring a therapeutic vulnerability in IDH1-mutant solid tumors.Mechanistically,SLC1 Alz a Na'-depe ndent glutamate tran sporter that is prefere ntially expressed in en dothelial cells,facilitates the in flux of R-2-HG from the tumor microenvironment into the endothelial cells as well as the intracellular trafficking of R-2-HG from cytoplasm to mitochondria.R-2-HG hijacks SLC1A1 to promote mitochondrial Na^(+)/Ca^(2+)exchange,which activates the mitochondrial respiratory chain and fuels vascular en dothelial cell migratio n in tumor an giogenesis.SLC1A1 deficiency in mice abolishes mlDHl-promoted tumor an giogenesis as well as the therapeutic benefit of mlDHl in hibitor in solid tumors.Moreover,we report that HH2301,a newly discovered mlDHl inhibitor,shows promising efficacy in treating IDH1-mutant cholangiocarcinoma in preclinical models.Together,we identify a new role of SLC1A1 as a gatekeeper of R-2-HG-mediated crosstalk between IDH1-mutant tumor cells and vascular en dothelial cells,and dem on strate the therapeutic potential of mlDHl in hibitors in treating IDH1-muta nt solid tumors via disrupting R-2-HG-promoted tumor angiogenesis. | Xiaomin Wang Ziqi Chen Jun Xu Shuai Tang Nan An Lei Jiang Yixiang Zhang Shaoying Zhang Qingli Zhang Yanyan Shen Shijie Chen Xiaojing Lan Ting Wang Linhui Zhai Siyuwei Cao Siqi Guo Yingluo Liu Aiwei Bi Yuehong Chen Xiameng Gai Yichen Duan Ying Zheng Yixian Fu Yize Li Liang Yuan Linjiang Tong Kun Mo Mingcheng Wang Shu-Hai Lin Minjia Tan Cheng Luo Yi Chen Jia Liu Qiansen Zhang Leping Li Min Huang | 2022 | Cell Research2022，32，7： | 2 |
| 19 | Alizarin-TiO2 LMCT Complex with Oxygen Vacancies:An Efficient Visible Light Photocatalyst for Cr(Ⅵ)Reduction显示文摘of main observation and conclusion The AL-P25 composites(AL=alizarin,P25=nano titanium dioxide)with oxygen vacancies were synthesized by a simple hydrothermal method and applied for the photoreduction of Cr(VI)under visible light.The AL-P25 composites were characterized by scanning electron microscopy,X-ray diffraction,X-ray photoelectron spectroscopy analysis,Fourier transform infrared spectrometer,thermo-gravimetric analysis,UV-vis diffuse reflectance spectroscopy,photoluminescence spectroscopy and electron paramagnetic resonance spectroscopy.The results suggested that the AL-P25 composites were successfully prepared,and alizarin was linked to P25 via Ti—O—C bonds.AL-P25 composites represented excellent photoreduction efficiency on Cr(VI)under visible light,which was 99.4%after 60 min of illumination.Finally,a photocatalytic mechanism of AL-P25 composites was proposed that ligand-metal charge transfer(LMCT)process between alizarin and P25 effectively enhances separation efficiency of photogenerated electrons and holes. | Meichen Li Yarn Li Jingyu Zhao Mingcheng Li Yongchuan Wu Ping Na | 2020 | Chinese Journal of Chemistry2020，38，11： | 2 |
| 20 | Joint estimation of Doppler centroid and rate for SAR with large range migration 显示文摘 | YU Mingcheng XU Jia PENG Yingning | 2007 | Radar Sonar g~ Navigation IET2007，1，3： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员