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| 1 | Preparation and activity of conjugate of monoclonal antibody HAb18 against hepatoma F( ab′ )_2 fragment and staphylococcal enterotoxin A显示文摘AIM To prepare the conjugate of staphylococcal enterotoxin A (SEA) protein which is a bacterial SAg and the F(ab')2 fragment of mAb HAbl8 against human hepatocellular carcinoma (HCC), and identify its activity in order to use SAg in the targeting therapy of HCC.METHODS MAb HAbl8 was extracted from the abdominal dropsy of Balb/ c mice, and was purified through chromatography column SP-40HR with Fast protein liquid chromatography (FPLC) system. The F(ab')2 fragment of mAb HAb18 was prepared by papainic digestion method. The conjugate of mAb HAb18 F(ab')2fragment and SEA was prepared with chemical conjugating reagent N-succinimidyl-3-( 2-pyridyldithio) propionate (SPDP) and purified through chromatography column Superose 12with FPLC system. The molecular mass and purity of each collected peak were identified with SDS-PAGE assay. The protein content was assayed by Lowry's method. The antibody activity of HAb18 F (ab')2 against HCC in the conjugate was identified by indirect immunocytochemical ABC method, and the activity of SEA in the conjugate to activate peripheral blood mononuclear cells (PBMC) was identified with MTT assay.RESULTS The lgG mAb HAb18 was extracted,and purified successfully. Immunocytochemical staining demonstrated that it reacted with most of HHCC cells of human HCC cell line. There were two peaks in the process of purification of the prepared HAb18 F(ab)2-SEA conjugate. SDS-PAGE assay demonstrated that the molecular mass of the first peak was about 130 ku, and the second peak was the mixture of about 45 ku and a little 100 ku proteins. The immunocytochemical staining was similar in HAb18 F (ab ')2-SEAconjugate and HAb18 F (ab ')2, i.e., thecytoplasm and/or cell membranes of most HHCC cells were positively stained. The MTT assay showed that the optical absorbance (A) value at 490 nm of HAb18 F (ab')2-SEA conjugate was 0.182 ± 0.012, that of negative control was 0.033± 0.009, and there was significant difference between them ( P < 0.05).CONCLUSION SPDP is a good proteinconjugating reagent and can be used in preparing protein conjugate. The conjugate of mAb HAb18F(ab')2 fragment and SEA protein was preparedsuccessfully in present study and can be used in the experimental study of HCC targeting therapy with the conjugate of SAg and anti-HCC mAbs or their fragments. | Lian Jun Yang Yan Fang Sui Zhi Nan Chen Department of Pathology, Fourth Military Medical University. Xi’an 710032, Shaanxi Province, China | 2001 | World Journal of Gastroenterology2001，7，2： | 20 |
| 2 | Plant phenological responses to climate change on the Tibetan Plateau: research status and challenges显示文摘Phenology studies the cycle of events in nature that are initiated and driven by an annually recurring environment. Plant phenology is expected to be one of the most sensitive and easily observable natural indicators of climate change. On the Tibetan Plateau(TP), an accelerated warming since the mid-1980 s has resulted in signiicant environmental changes. hese new conditions are accompanied by phenological changes that are characterized by considerable spatiotemporal heterogeneity. Satellite remote sensing observed widespread advance in the start of the plant growing season across the plateau during the 1980 s and 1990 s but substantial delay over 2000–2011 in the southwest although it continued to advance in the northeast regions of the TP. Both observational studies and controlled experiments have revealed, to some extent, the positive role of higher preseason temperature and even more precipitation in advancing the leaf onset and irst lowering date of the TP. However, a number of rarely visited research issues that are essential for understanding the role of phenology in ecosystem responses and feedback processes to climate change remain to be solved. Our review recommends that addressing the following questions should be a high priority. How did other phenological events change, such as lowering and fruiting phenology? What are the inluences from environmental changes other than temperature and precipitation, including human activities such as grazing? What are the genetic and physiological bases of plants phenological responses?How does phenological change inluence ecosystem structure and function at diferent scales and feedback to the climate system? Investigating these research questions requires, irst of all, new data of the associated environmental variables, and consistent and reliable phenological observation using diferent methodologies(i.e. in situ observations and remote sensing). | Miaogen Shen Shilong Piao Tsechoe Dorji Qiang Liu Nan Cong Xiaoqiu Chen Shuai An Shiping Wang Tao Wang Gengxin Zhang | 2015 | National Science Review2015，2，4： | 13 |
| 3 | Serum N-glycan markers for diagnosing liver fibrosis induced by hepatitis B virus显示文摘BACKGROUND Hepatitis B virus (HBV) infection is the primary cause of hepatitis with chronic HBV infection,which may develop into liver fibrosis,cirrhosis and hepatocellular carcinoma.Detection of early-stage fibrosis related to HBV infection is of great clinical significance to block the progression of liver lesion.Direct liver biopsy is regarded as the gold standard to detect and assess fibrosis;however,this method is invasive and prone to clinical sampling error.In order to address these issues,we attempted to find more convenient and effective serum markers for detecting HBV-induced early-stage liver fibrosis.AIM To investigate serum N-glycan profiling related to HBV-induced liver fibrosis and verify multiparameter diagnostic models related to serum N-glycan changes.METHODS N-glycan profiles from the sera of 432 HBV-infected patients with liver fibrosis were analyzed.Significant changed N-glycan levels (peaks)(P <0.05) in differentfibrosis stages were selected in the modeling group,and multiparameter diagnostic models were established based on changed N-glycan levels by logistic regression analysis.The receiver operating characteristic (ROC) curve analysis was performed to evaluate diagnostic efficacy of N-glycans models.These models were then compared with the aspartate aminotransferase to platelet ratio index (APRI),fibrosis index based on the four factors (FIB-4),glutamyltranspeptidase platelet albumin index (S index),GlycoCirrho-test,and GlycoFibro-test.Furthermore,we combined multiparameter diagnostic models with alanine aminotransferase (ALT) and platelet (PLT) tests and compared their diagnostic power.In addition,the diagnostic accuracy of N-glycan models was also verified in the validation group of patients.RESULTS Multiparameter diagnostic models constructed based on N-glycan peak 1,3,4and 8 could distinguish between different stages of liver fibrosis.The area under ROC curves (AUROCs) of Model A and Model B were 0.890 and 0.752,respectively differentiating fibrosis F0-F1 from F2-F4,and F0-F2 from F3-F4,and surpassing other serum panels.However,AUROC (0.747) in Model C used for the diagnosis of F4 from F0-F3 was lower than AUROC (0.795) in FIB-4.In combination with ALT and PLT,the multiparameter models showed better diagnostic power (AUROC=0.912,0.829,0.885,respectively) when compared with other models.In the validation group,the AUROCs of the three combined models (0.929,0.858,and 0.867,respectively) were still satisfactory.We also applied the combined models to distinguish adjacent fibrosis stages of 432patients (F0-F1/F2/F3/F4),and the AUROCs were 0.917,0.720 and 0.785.CONCLUSION Multiparameter models based on serum N-glycans are effective supplementary markers to distinguish between adjacent fibrosis stages of patients caused by HBV,especially in combination with ALT and PLT. | Xi Cao Qing-Hua Shang Xiao-Ling Chi Wei Zhang Huan-Ming Xiao Mi-Mi Sun Gang Chen Yong An Chun-Lei Lv Lin Wang Yue-Min Nan Cui-Ying Chen Zong-Nan Tan Xue-En Liu Hui Zhuang | 2020 | World Journal of Gastroenterology2020，26，10： | 12 |
| 4 | An overview of design for CSNS/RCS and beam transport显示文摘The China Spallation Neutron Source (CSNS) is the first accelerator-based pulsed neutron source in China. Its accelerators are made up of an 80 MeV H- linac, a Rapid Cycling Synchrotron (RCS) and two beam transport lines. RCS accumulates and accelerates protons to the design energy of 1.6 GeV, and extracts high energy beam to strike the target. The overview of RCS is presented, and the key problems of the physics design are discussed. The two beam transport lines, from linac to RCS and from RCS to the target, are also introduced. | WANG Sheng AN YuWen FANG ShouXian HUANG Nan LIU WeiBin LIU YuDong QIN Qing QIU Jing WANG Na XU Gang XU ShouYan YU ChengHui | 2011 | Science China(Physics,Mechanics & Astronomy)2011，54，S2： | 11 |
| 5 | A LysM Receptor Heteromer Mediates Perception of Arbuscular Mycorrhizal Symbiotic Signal in Rice显示文摘Symbiotic microorganisms improve nutrient uptake by plants.To initiate mutualistic symbiosis with arbus-cular mycorrhizal(AM)fungi,plants perceive Myc factors,including lipochitooligosaccharides(LCOs)and short-chain chitooligosaccharides(CO4/CO5),secreted by AM fungi.However,the molecular mechanism of Myc factor perception remains elusive.In this study,we identified a heteromer of LysM receptor-like kinases consisting of OsMYR1/OsLYK2 and OsCERK1 that mediates the perception of AM fungi in rice.CO4 directly binds to OsMYR1,promoting the dimerization and phosphorylation of this receptor complex.Compared with control plants,Osmyr1 and Oscerk1 mutant rice plants are less sensitive to Myc factors and show decreased AM colonization.We engineered transgenic rice by expressing chimeric receptors that respectively replaced the ectodomains of OsMYR1 and OsCERK1 with those from the homologous Nod factor receptors MtNFP and MtL YK3 of Medicago truncatula.Transgenic plants displayed increased cal-cium oscillations in response to Nod factors compared with control rice.Our study provides significant mechanistic insights into AM symbiotic signal perception in rice.Expression of chimeric Nod/Myc recep-tors achieves a potentially important step toward generating cereals that host nitrogen-fixing bacteria. | Jiangman He Chi Zhang Huiling Dai Huan Liu Xiaowei Zhang Jun Yang Xi Chen Yayun Zhu Dapeng Wang Xiaofeng Qi Weichao Li Zhihui Wang Guoyong An Nan YU Zuhua He Yong-Fei Wang Youli Xiao Peng Zhang Ertao Wang | 2019 | Molecular Plant2019，12，12： | 9 |
| 6 | Impact of CYP3A4*1G Polymorphism on Fentanyl Analgesia Assessed by Analgesia Nociception Index in Chinese Patients Undergoing Hysteroscopy显示文摘Background:The clinical efficacy of fentanyl for pain control differs greatly across individuals.The purpose of this study was to investigate the impact of CYP3A4*1G polymorphism including wild-type homozygote (CYP3A4*1/*1,GG),mutant heterozygote (CYP3A4*1/*1G,GA),and mutant homozygote (CYP3A4*1G/*1G,AA)on fentanyl analgesia in Chinese patients undergoing hysteroscopy by the assessment of analgesia nociception index (ANI). Methods:A total of 200 gynecologic patients scheduled for elective hysteroscopy under general anesthesia at Peking University People's Hospital from May to December in 2017 were enrolled in this study.Venous blood was withdrawn for genotyping of CYP3A4*1G before operation.Fentanyl 1μg/kg was administered preoperatively followed by target-controlled infusion of propofol for induction and maintenance.Intraoperative analgesic efficacy of fentanyl was assessed by ANI monitoring at T0(entering room),T1(cervical dilation), T2(start of cervical aspiration),and T3(end of cervical aspiration)time points.The duration of propofol infusion and total dosage of propofol were recorded as well. Results:The patients were divided into three groups according to CYP3A4*1G polymorphism,including 143 in GG group,47in GA group, and 10 in AA group.There was no significant difference in clinical demographics among three groups.The frequency of CYP3A4*1G variant alleles accounted for 16.8%and the distribution of variant alleles was consistent with Hardy-Weinberg equilibrium.Using a multilevel model,ANI values at T1(63.81±19.61),T2(63.63±17.82),and T3(65.68±17.79)were significantly lower than that at T0 (77.16±12.93)in the study population (F =23.50,P <0.001),suggesting that higher levels of pain at T 1,T2,and T3 than T0.Patients with GG genotype showed significantly lower ANI than those with GA or AA genotypes during hysteroscopy under the same dose of fentanyl. Conclusion:CYP3A4*1G polymorphism associated with the analgesic efficacy of intraoperative fentanyl in the patients undergoing hysteroscopy under general anesthesia. | Qi Yan Yi Su Lan Gao Nan Ding Hong-Ying Zhang Wen E Yue Wang Yi Feng Hai-Yan An | 2018 | Chinese Medical Journal2018，，22： | 8 |
| 7 | Reliable grading robust stabilization for uncertain time-varying systems via dynamic compensator显示文摘A new general model for uncertain time-varying parameters and a new measure sensor failure model are presented, and the problems of both grading robust stabilization and reliable grading robust stabilization for such systems are studied. By the Lyapunov stability theory and matrix algebra method, some sufficient criteria for the above two control problems are established in quasi-linear matrix inequalities (Q-LMIS) forms. In view of linear matrix inequality (LMI) approach, a solving procedure for the Q-LMIS problem is proposed. The solvability of the Q-LMIS problem can be improved obviously by adding some LMI constraints to the Q-LMIS. Based on the two Q-LMIS criteria, a grading robust stable control strategy, namely, the controller with different energy is acted on the system with different uncertain parameter range, is presented. The numerical simulating results show that the grading robust stable control strategy for the robust stabilization of uncertain systems has important theoretical and practical significance. | JIA Xinchun and ZHENG Nanning(Institute of Artificial Intelligence and Robotics, Xi’an Jiaotong University, Xi’an 710049, China) | 2002 | Progress in Natural Science:Materials International2002，12，10： | 7 |
| 8 | Mesozoic basins and associated palaeogeographic evolution in North China显示文摘In North China, the Mesozoic terrestrial basins, sedimentary palaeogeography and tectonic settings involved five evolutionary stages:(1) the Early-Middle Triassic,(2) the Late Triassic to Early-Middle Jurassic,(3) the Late Jurassic to early Early Cretaceous,(4) the middle-late Early Cretaceous and(5) the Late Cretaceous. The regional punctuated tectonic events occurred during these evolutionary stages. During the Early-Middle Triassic(stage 1), the Xingmeng Orogenic Belt(XMOB, i.e., eastern part of Central Asia Orogenic Belt, CAOB) of the northern North China was settled in the transition of tectonic environment from syn-orogenic compression to post-orogenic extension with intensive uplifting. It is a main provenance in the unified Ordos-North China Basin.The united continental plate of China and the Qinling-Dabie-Sulu Orogenic Belt formed due to convergence and collision between the North China Plate and the Yangtze Plate along two suture zones of the Mianlue and the Shangdan in the terminal Middle Triassic. During the Late Triassic to the Early-Middle Jurassic(stage 2), the Late Triassic mafic or alkaline rocks and intrusions occurred on the northern and southern margins of North China Craton(NCC) and XMOB, implying that intensified extension happened all over the North China(early phase of stage 2). Additionally, in the late phase of stage 2, the basic volcanicfilling faulted basins were widely distributed in the northeastern North China during the EarlyMiddle Jurassic, including a series of small- to medium-sized basins with coal-bearing strata and some volcanic rocks in other areas of North China, which was the result of subduction of the Palaeo-Pacific Plate during the Early-Middle Jurassic. An active continental margin with accretionary complex developed in the eastern Heilongjiang of China, Japan and the Far East of Russia at that time. However, in the end of the Early-Middle Jurassic, because of the Yanshanian orogeny characterized by complicated thrust and fold, the previous unified OrdosNorth China Basin was separated by the northeast-oriented Great Xing'an Range and Taihang Mountain uplifted linearment. The differential evolution of basins and sedimentary palaeogeography between eastern and western North China was initiated, and was interpreted to result in the closure of Okhotsk Ocean and the subduction of Palaeo-Pacific Plate(late stage 2). During the Late Jurassic(the early phase of stage 3), a variety of faulted basins occurred in the Yanshan and Yinshan areas in the northeastern North China. In Yanshan area, basins were filled with thickened intermediate volcanic rocks and purple-red coarse-grained clastic rocks. In contrast, only thick layered sedimentary rocks with rare volcanic rocks developed in the Yinshan faulted basins, the Ordos Basin and basins in sourthern North China. XMOB wasthe main provenance of the Early Mesozoic basins in the North China, while the Ordos Basin and the Hefei Basin were partly supplied by the northern Qinling Orogenic Belt. During the Late Jurassic-early Early Cretaceous(the late phase of stage 3), the northern and northeastern North China experienced extensional movement after the subduction of the Palaeo-Pacific Plate, the closure of the Mongolia-Okhotsk Ocean and the subsequent Yanshanian orogeny. At the same time, a NE-oriented, giant rift basin system(NE Asia Rift) extended from the Yanshan to the western Great Xing'an Range, where rift basins were filled with the regional, NE-oriented, thick coarse-grained clastic rocks and a belt of volcanic rocks. In the meantime, the eastern and northeastern China and most areas of NCC were presented as highland terrains. During the middle-late Early Cretaceous(stage 4), rift basins developed and accumulated alluvial sediments and interbedded alkaline volcanic rocks in the western and northern North China, including Yingen, Ejinaqi and Erlian regions. Basins were formed on both sides of the Tan-Lu Fault Zone under a striking-slipping force. Furthermore, faulted basins developed in the Yishu Fault Zone of Shandong(central Tan-Lu Fault Zone) as well, where dinosaur fauna flourished. Basic volcanic rocks and fluvial-lacustrine sediments were deposited in small- or medium-sized rift basins in the northeastern China. The Songliao Basin was a typical giant basin that was mainly filled with late Early Cretaceous lacustrine sediments. A group of rift basins occurred in the Sanjiang area, central Heilongjiang Province, northeastern China. From the middle-late Early Cretaceous to the Late Cretaceous(stage 5), depositional and subsiding center of the basins constantly shifted southeastwards in Heilongjiang Province. The tectonic setting changed into the Palaeo-Pacific continental margin in north and northeastern China. Besides, during the Late Mesozoic, a huge terrestrial biota, mainly dinosaur fauna, dominated in North China. The Yanliao biota of the Middle-Late Jurassic and the Jehol biota of the Early Cretaceous are characterized by feathered dinosaurs, primitive birds, mammals, pterosaur, insects and plants(angiosperms). In northeastern Asia, this Late Mesozoic tectonic background, palaeogeoraphy and palaeoecology were shared by East China, Korean Peninsula, Japan and the Far East of Russia. | Yong-Qing Liu Hong-Wei Kuang Nan Peng Huan Xu Peng Zhang Neng-Sheng Wang Wei An | 2015 | Journal of Palaeogeography2015，4，2： | 7 |
| 9 | Emission inventory evaluation using observations of regional atmospheric background stations of China显示文摘Any accurate simulation of regional air quality by numerical models entails accurate and up-to-date emissions data for that region.The INTEX-B2006 (I06),one of the newest emission inventories recently popularly used in China and East Asia,has been assessed using the Community Multiscale Air Quality model and observations from regional atmospheric background stations of China.Comparisons of the model results with the observations for the species SO2,NO 2,O 3 and CO from the three regional atmospheric background stations of Shangdianzi,Longfengshan and Linan show that the model can basically capture the temporal characteristics of observations such as the monthly,seasonal and diurnal variance trends.Compared to the other three species,the simulated CO values were grossly underestimated by about two-third or one-half of the observed values,related to the uncertainty in CO emissions.Compared to the other two stations,Shangdianzi had poorer simulations,especially for SO2 and CO,which partly resulted from the site location close to local emission sources from the Beijing area;and the regional inventory used was not capable of capturing the influencing factors of strong regional sources on stations.Generally,the fact that summer gave poor simulation,especially for SO2 and O 3,might partly relate to poor simulations of meteorological fields such as temperature and wind. | Xingqin An Zhaobin Sun Weili Lin Min Jin Nan Li | 2013 | Journal of Environmental Sciences2013，25，3： | 7 |
| 10 | N-doped carbon nanocages: Bifunctional electrocatalysts for the oxygen reduction and evolution reactions显示文摘为氧进化反应(OER ) 和氧减小反应(ORR ) 的高度有效的没有金属的、基于碳的、双性人功能的 electrocatalysts 在电气化学的精力变换系统为使用吸引了增加的注意,由于他们的低费用和高活动。在这个工作,有多孔的 self-supported 体系结构和高特定的表面区域的做 N 的碳 nanocages (N-CCs ) 被一个灵巧的界面的集会综合合成线路。材料被扫描电子显微镜学,传播电子显微镜学,氮吸附解吸附作用实验, X 光检查衍射,和 X 光检查光电子光谱学包括地描绘。周期的 voltammetry, chronoamperometry,并且线性打扫 voltammetry 证明同样准备的 N-CC 能在碱的媒介为 ORR 与优秀催化活动,长期的操作耐久性,和优秀甲醇忍耐用作有效没有金属的 electrocatalyst。面对 3 公里甲醇,一半为 ORR 的 N-CCs 的波浪潜力是 190 mV;这比商业 Pt/C electrocatalyst 的更积极。同时, N-CCs 也显示出比得上商业 Ru/C electrocatalyst 的一项 OER 活动,揭示他们的 bifunctional 性质。 | Nan Jia Qiang Weng Yaru Shi Xinyan Shi Xinbing Chen Pei Chen Zhongwei An Yu Chen | 2018 | Nano Research2018，11，4： | 6 |
| 11 | Higher-Ordered Actin Structures Remodeled by Arabidopsis ACTIN-DEPOLYMERIZING FACTOR5 Are Important for Pollen Germination and Pollen Tube Growth显示文摘肌动朊细胞骨架的动力学为花粉萌芽和花粉试管生长是必要的。使解聚肌动朊的因素(ADF ) 典型地由切断 / 使解聚肌动朊细丝贡献肌动朊周转。最近,我们证明 Arabidopsis 亚纲 III ADF (ADF5 和 ADF9 ) 从保存 F-actin-depolymerizing 功能发展了 F-actin-bundling 功能。然而,很少对生理的功能,进化意义,和这些 neofunctionalized ADF 的肌动朊捆绑机制被知道。这里,我们报导 ? 引起的 ADF5 功能的那损失推迟了花粉萌芽,延迟的花粉试管生长,并且增加对由影响肌动朊捆的产生和维护的 latrunculin B (LatB ) 处理敏感。在生活房间的肌动朊细丝动力学的检查表明捆绑频率显著地在 adf5 花粉试管被减少,与它的生物化学的功能一致。进一步生物化学、基因的互补分析 ? 两个都表明了那 N-? 并且 ADF5 的 C 终端肌动朊绑定域为它的生理、生物化学的函数被要求。有趣地,当两个是不正常的肌动朊捆绑 ADF, ADF5,然而并非 ADF9 时,在成熟花粉起一个重要作用生理的活动。一起拿,我们的结果建议 ADF5 发展了捆绑肌动朊细丝的功能并且在花粉萌芽和花粉试管生长期间在肌动朊捆的形成,组织,和维护起一个重要作用。 | Jingen Zhu Qiong Nan Tao Qin Dong Qian Tonglin Mao Shunjie Yuan Xiaorong Wu Yue Niu Qifeng Bai Lizhe An Yun Xiang | 2017 | Molecular Plant2017，10，8： | 6 |
| 12 | Quantitative method to determine the regional drinking water odorant regulation goals based on odor sensitivity distribution:Illustrated using 2-MIB显示文摘Taste and odor(T/O) in drinking water often cause consumer complaints and are thus regulated in many countries. However, people in different regions may exhibit different sensitivities toward T/O. This study proposed a method to determine the regional drinking water odorant regulation goals(ORGs) based on the odor sensitivity distribution of the local population. The distribution of odor sensitivity to 2-methylisoborneol(2-MIB) by the local population in Beijing, China was revealed by using a normal distribution function/model to describe the odor complaint response to a 2-MIB episode in 2005, and a 2-MIB concentration of12.9 ng/L and FPA(flavor profile analysis) intensity of 2.5 was found to be the critical point to cause odor complaints. Thus the Beijing ORG for 2-MIB was determined to be 12.9 ng/L. Based on the assumption that the local FPA panel can represent the local population in terms of sensitivity to odor, and that the critical FPA intensity causing odor complaints was 2.5, this study tried to determine the ORGs for seven other cities of China by performing FPA tests using an FPA panel from the corresponding city. ORG values between 12.9 and 31.6 ng/L were determined, showing that a unified ORG may not be suitable for drinking water odor regulations. This study presents a novel approach for setting drinking water odor regulations. | Jianwei Yu Wei An Nan Cao Min Yang Junong Gu Dong Zhang Ning Lu | 2014 | Journal of Environmental Sciences2014，26，7： | 5 |
| 13 | An Effective Over-sampling Method for Imbalanced Data Sets Classification显示文摘 | ZHAI Yun MA Nan RUAN Da AN Bing | 2011 | Chinese Journal of Electronics2011，20，3： | 5 |
| 14 | Application of UPLC-MS/MS Method for Analyzing B-vitamins in Human Milk显示文摘Objective To determine ten B-vitamins in human milk by ultra-high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS). Methods The pretreated human milk samples were adequately separated and quantified within 11 min by UPLC-MS/MS with an Acquity UPLC HSS T3 column(2.1×100 mm, 1.8 μm). The mobile phase was a gradient of 2.5 mmol/L ammonium formate aqueous solution and acetonitrile at a flow rate of 0.35 m L/min. Stable isotope internal standards were used in the analysis, to correct for the method variability, including matrix and ionization effects. The homogenized human milk samples were deproteinzed using methanol, unknown contaminants were extracted with diethyl ether and hydrophobic phase was discarded. The analytes were monitored via ESI+ionization and detected in multiple reaction monitoring(MRM) with three acquisition functions. Results Calibration curves ranged from 0.5-160 ng/m L(thiamin, riboflavin, biotin, nicotinic acid, pyridoxine, pyridoxamine, pyridoxal), and 2.5-800 ng/m L(pantothenic acid, FAD and nicotinamide)(R2=0.990-0.999). The relative recovery ranged from 80.1% to 120.2%; accuracy was determined to be 98.3% to 108.0%. Intra-day and inter-day variation were 3.4%-19.9% and 5.9%-18.1%, respectively. The limit of quantification(LOQ) for all vitamins was between 0.25 and 3 μg/L. Conclusion This method was successfully applied for simultaneous analysis of ten B-vitamins in human milk. | REN Xiang Nan YIN Shi An YANG Zhen Yu YANG Xiao Guang SHAO Bing REN Yi Ping ZHANG Jing | 2015 | Biomedical and Environmental Sciences2015，28，10： | 4 |
| 15 | Prognostic value of dynamic electroencephalogram in comatose patients with different diseased regions显示文摘BACKGROUND: It has proved that dynamic electroencephalogram (EEG) is definite in judging the outcome of ischemic hypoxic comatose patients, EEG is more sensitive to the cortical affection, but not sensitive to the subcortical and brainstem affections, thus it is necessary to clarify the indications of this technique in the clinical application. OBJECTIVE: To observe and compare the prognostic value of dynamic EEG and Glasgow coma score in comatose patients with different diseased region. DESIGN: A clinical case-controlled observation. SETTING: Union Hospital of Fujian Medical University. PARTICIPANTS: Sixty-eight comatose patients were selected from the Union Hospital affiliated to Fujian Medical University from June 1998 to January 2005. The diseased regions were identified using cranial CT (n =43) or MR (n =25). According to different primarily diseased regions, the comatose patients were divided into two groups: ① brainstem affection group (n =23): 13 males and 10 females, 14–62 years of age; ② diffuse cortical affection group (n =45): 28 males and 17 females, 23–75 years of age. METHODS: The dynamic EEG and Glasgow coma score were examined in the 45 comatose patients with primarily cortical affection and 22 comatose patients with primarily brainstem affection at acute phase. The patients were followed-up for 3 months to observe the outcome, The termination of outcome judgment was 3 months after attack or the death. The clinical outcome was classified as complete rehabilitation, survived with disability, death or vegetative state. Correlations of dynamic EEG and Glasgow coma score with the outcome of patients were analyzed. The correlations of dynamic EEG grades and Glasgow coma scores with the outcome were analyzed, and the prognostic value of dynamic EEG grades was compared between the two groups. MAIN OUTCOME MEASURES: ① Correlations of dynamic EEG and Glasgow coma score with the outcome of patients; ② Comparison of the prognostic value of dynamic EEG grades between the two groups. RESULTS: All the 68 patients were involved in the analysis of results. ① Correlations of dynamic EEG grades and Glasgow scores and their correlation analysis: EEG grades had significant negative correlation with Glasgow coma scores in both the cortical affection group and brainstem affection group (r =–0.743, –0.564, P < 0.01, 0.05). In the cortical affection group, the Glasgow coma scores and dynamic EEG grades in the patients with the outcome of death or vegetative state were significantly different from those with the outcome of rehabilitation (P < 0.05–0.01). In the brainstem affection group, the Glasgow coma scores were only significantly different between the patients with outcome of rehabilitation and death (P < 0.05), and there was no significant difference in dynamic EEG grades among the three prognostic states (P > 0.05). ② Comparison of the prognostic value of dynamic EEG grades between comatose patients with cortical affection and brainstem affection: The sensitivity, specificity and accuracy were all higher (P < 0.05), while the error rate was lower (P < 0.05) in the cortical affection group than in the brainstem affection group. CONCLUSION: Dynamic EEG was valuable in predicting the outcome of comatose patients with primarily cortical affection, but it was not certainly valuable in those with primarily brainstem affection. | Huapin Huang Chunhui Che An Zheng Xiaoyun Zhuang Fang Jiang Nan Liu | 2007 | Neural Regeneration Research2007，2，7： | 4 |
| 16 | A preliminary study of genes related to concomitant chemoradiotherapy resistance in advanced uterine cervical squamous cell carcinoma显示文摘 | An Ju-sheng Huang Man-ni Song Yong-mei Li Nan Wu Ling-ying Zhan Qi-min | 2013 | Chinese Medical Journal2013，，21： | 4 |
| 17 | Overlying strata movement of recovering standing pillars with solid backfilling by physical simulation显示文摘To analyze the overlying strata movement law of recovering room mining standing pillars with solid backfilling.Physical simulation experiments with sponge and wood as the backfilling simulation material were tested.The results show that:(i) The covering-rock mechanics of the overly strata comes from 'two-arch structures + hinged girder + bend beam' to 'backfilling material + hinged girder + bent beam' by increasing the fill ratio from 0%to 85%,the beginning of overlying strata movement appears later and the total duration of subsidence velocity increased from zero to the highest value increases.The trend of 'single polarization' of the subsidence velocity curves becomes noticeable and the velocity variation trend becomes stable,(ii) The equiponderate aeolian sand was added to improve the anti-pressure ability of the loess,and the corresponding ground processing & transportation system was designed. | An Baifu Miao Xiexing Zhang Jixiong Ju Feng Zhou Nan | 2016 | International Journal of Mining Science and Technology2016，26，2： | 3 |
| 18 | First experimental constraints on WIMP couplings in the effective field theory framework from CDEX显示文摘We present weakly interacting massive particles(WIMPs) search results performed using two approaches of effective field theory from the China Dark Matter Experiment(CDEX), based on the data from both CDEX-1B and CDEX-10 stages. In the nonrelativistic effective field theory approach, both time-integrated and annual modulation analyses were used to set new limits for the coupling of WIMP-nucleon effective operators at 90% confidence level(C.L.) and improve over the current bounds in the low mχregion. In the chiral effective field theory approach, data from CDEX-10 were used to set an upper limit on WIMP-pion coupling at 90% C.L. We for the first time extended the limit to the m_(χ)<6 GeV/c^(2) region. | Yi Wang Zhi Zeng Qian Yue LiTao Yang KeJun Kang YuanJing Li Mehment Agartioglu HaiPeng An JianPing Chang JingHan Chen YunHua Chen JianPing Cheng Cheng Yi Chiang WenHan Dai Zhi Deng ChangHao Fang XinPing Geng Hui Gong QiuJu Guo XuYuan Guo HongJian He Li He ShengMing He JinWei Hu TuChen Huang HanXiong Huang HaiTao Jia LiPing Jia Xi Jiang HauBin Li JianMin Li Jin Li MingXuan Li RenMingJie Li Xia Li YuLan Li Bin Liao FongKay Lin ShinTed Lin ShuKui Liu YanDong Liu Yu Liu YuanYuan Liu ZhongZhi Liu Hao Ma YuCai Mao QiYuan Nie JinHua Ning Hui Pan NingChun Qi Jie Ren XiChao Ruan ChangSong Shang Vivek Sharma Ze She Lakhwinder Singh Monoj Kumar Singh TianXi Sun ChangJian Tang WeiYou Tang Yang Tian GuangFu Wang Li Wang Qing Wang Yu Chen Wang YunXiang Wang Zhen Wang Henry Tsz-King Wong ShiYong Wu YuCheng Wu HaoYang Xing Yin Xu Tao Xue YuLu Yan Nan Yi ChunXu Yu HaiJun Yu JianFeng Yue Ming Zeng BingTao Zhang Lei Zhang FengShou Zhang ZhenYu Zhang KangKang Zhao MingGang Zhao JiFang Zhou ZuYing Zhou JingJun Zhu | 2021 | Science China(Physics,Mechanics & Astronomy)2021，64，8： | 3 |
| 19 | Detection and Initial Response to a Type 2 Vaccine-Derived Poliovirus—Sichuan Province,China,2019显示文摘What is already known about this topic?After the type 2 strain of the live,attenuated poliovirus vaccine was withdrawn globally in 2016,any identification of a type 2 poliovirus is a Public Health Emergency of International Concern.A vaccinederived type 2 poliovirus(VDPV2)was identified in Sichuan,prompting an urgent,comprehensive investigation and response.What is added by this report?Type 2 monovalent,live,attenuated oral poliovirus vaccine(mOPV2)is being used to respond to the numerous VDPV2 outbreaks seen around the world.In contrast,the response in Sichuan used Sabin strain inactivated poliovirus(sIPV)to stop circulation of the VDPV2.In the 6 months following the vaccination response,there have been no VDPV2s detected in Sichuan,despite extensive search.What are the implications for public health practices?Further search for the VDPV2 must continue in order to determine whether transmission has been stopped.The ongoing investigation and response to the Sichuan VDPV2 is providing evidence to the Global Polio Eradication Initiative on managing VDPV2 outbreaks. | Jiushun Zhou Ning Wen Yong Zhang Qi Qi Chunxiang Fan Dongmei Yan Xiaoping Zhu Lixin Hao Shuangli Zhu Yu Liu Xiaozhen Ma Chao Ma Lei Nan Yong Chen Qianli Ma Cheng Wang Kun Deng Lei Cao Ge Shao Xianxiang Ding Hong Yang Zhijie An Lance ERodewald Aiqiang Xu Huaqing Wang Zijian Feng Zundong Yin Xianping Wu Wenbo Xu | 2020 | China CDC weekly2020，2，11： | 3 |
| 20 | Ten‐Day Quadruple Therapy Comprising Proton‐Pump Inhibitor, Bismuth, Tetracycline, and Levofloxacin Achieves a High Eradication Rate for H elicobacter pylori Infection after Failure of Sequential Therapy显示文摘 | Ping‐I Hsu Wen‐Chi Chen Feng‐Woei Tsay Chih‐An Shih Sung‐Shuo Kao Huay‐Min Wang Hsien‐Chung Yu Kwok‐Hung Lai Hui‐Hwa Tseng Nan‐Jing Peng Angela Chen Chao‐Hung Kuo Deng‐Chyang Wu | 2014 | Helicobacter2014，，1： | 3 |
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