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| 1 | Generation and characterization of stable pig pregastrulation epiblast stem cell lines显示文摘Pig epiblast-derived pluripotent stem cells are considered to have great potential and broad prospects for human therapeutic model developme nt and livestock breeding.Despite on going attempts since the 1990s,no stably defined pig epiblast-derived stem cell line has bee n established.Here,guided by in sights from a large-scale sin gle-cell tran scriptome an alysis of pig embryos from embryonic day(E)0 to E14,specifically,the tracing of pluripotency changes during epiblast development,we developed an in vitro culture medium for establishing and maintaining stable pluripotent stem cell lines from pig E10 pregastrulation epiblasts(pgEpiSCs).Enabled by chemical inhibition of WNT-related signaling in combination with growth factors in the FGF/ERK,JAK/STAT3,and Activin/Nodal pathways,pgEpiSCs maintain their pluripotency transcriptome features,similar to those of E10 epiblast cells,and normal karyotypes after more than 240 passages and have the potential to differentiate into three germ layers.Strikingly,ultradeep in situ Hi-C analysis revealed functional impacts of chromatin 3D-spatial associations on the transcriptional regulation of pluripote ncy marker genes in pgEpiSCs.I n practice,we con firmed that pgEpiSCs readily tolerate at least three rounds of successive gene editi ng and gene rated cloned gen e-edited live piglets.Our findings deliver on the long-a nticipated promise of pig pluripote nt stem cells and open new avenues for biological research,animal husbandry,and regenerative biomedicine. | Minglei Zhi Jinying Zhang Qianzi Tang Dawei Yu Shuai Gao Dengfeng Gao Pengliang Liu Jianxiong Guo Tang Hai Jie Gao Suying Cao Zimo Zhao Chongyang Li Xiaogang Weng Mengnan He Tianzhi Chen Yingjie Wang Keren Long Deling Jiao Guanglei Li Jiaman Zhang Yan Liu Yu Lin Daxin Pang Qianqian Zhu Naixin Chen Jingjing Huang Xinze Chen Yixuan Yao Jingcang Yang Zicong Xie Xianya Huang Mengxin Liu Ran Zhang Qiuyan Li Yiliang Miao Jianhui Tian Xingxu Huang Hongsheng Ouyang Bofeng Liu Wei Xie Qi Zhou Zhonghua Liu Caihong Zheng Mingzhou Li Jianyong Han | 2022 | Cell Research2022，32，4： | 3 |
| 2 | Experimental far-field imaging properties of high refractive index microsphere lens显示文摘The far-field imaging properties of a high index microsphere lens spatially separated from the object are experimentally studied. Our experimental results show that, for a Blu-ray disk whose spacing is 300 nm, the high index microsphere lens also can discern the patterns of the object sample when the distance between the lens and the object is up to 5.4 μm. When the distance is increased from 0 to 5.4 μm, for the microsphere lens with a diameter of 24 μm, the lateral magnification increases from 3.5× to 5.5×, while the field of view decreases from 5.1 to 3.0 μm. By varying the distance between the lens and the object, the optical image can be optimized. We also indicate that the far-field imaging capability of a high index microsphere lens is dependent on the electromagnetic field intensityprofile of the photonic nanojet under different positions of the microsphere lens. | Minglei Guo Yong-Hong Ye Jinglei Hou Bintao Du | 2015 | Photonics Research2015，3，6： | 3 |
| 3 | Progress in the beam commissioning of separated function RFQ accelerator显示文摘Separated Function RFQ (SFRFQ) was proposed as a post accelerator of RFQ to accelerate heavy ions at low frequency. It introduces gap accelerating in the quadrupole electrodes, and therefore it has higher accelerating efficiency than the conventional RFQ accelerator. The first SFRFQ prototype cavity has been specially designed and constructed as a post accelerator to accelerate O+ beam from 1.03 MeV to 1.64 MeV. Based on accomplishment of low power measurement and high power test, the beam commissioning was carried out to verify its feasibility. The measured energy gain per cell of SFRFQ is 45 keV, which is about 60% higher than that of Peking University Integral Split Ring (ISR) RFQ. | MingLei Kang (1) YuanRong Lu (1) Zhi Wang (1) Kun Zhu (1) XueQing Yan (1) ZhiYu Guo (1) ShuLi Gao (1) ShiXiang Peng (1) Ao Liu (2) JiaXun Fang (1) JiaEr Chen (1) | 2011 | Science China(Physics,Mechanics & Astronomy)2011，54，S2： | 2 |
| 4 | Study on Preventive Effect of Cecropin and Lysozyme on Skin Fester Disease in Trionyx sinensis显示文摘[Objective] This study was to investigate the preventive effect of cecropin and lysozyme on skin fester disease in Trionyx sinensis.[Method]By employing intramuscular injection method,the preventive effect of mixtures of cecropin( 10 mg/kg) and lysozyme( 10 mg/kg) and of cecropin( 6 mg/kg) and lysozyme( 5 mg/kg) were challenged on Aeromonas encheleia-caused skin fester disease in T.sinensis.The mortality and relative percent survival were used evaluate the preventive effect of different drugs.The serum agglutination titer,cytophagocytosis and bactericidal activity were detected to compare the effect of drugs on the immunity and disease resistance of T.sinensis.[Result]The residue concentrations of gentamicin and lysozyme in serum were compared to evaluate the side effect of drugs used challenged.The results showed that combined use of cecropin and lysozyme gave the best preventive effect and immunity in T.sinensis,with an extremely significant difference from other treatments( P < 0.01).Analysis of drug residue in serum indicated that lysozyme was completely metabolized 6 days after medication,while gentamicin residue was still observed 12 days later.[Conclusion] The combined application of cecropin and lysozyme showed better preventive effect than antibiotics in fester disease of T.sinensis.Moreover,no drug residue and side effect were observed in the combined use treatment.This method avoids the side effect of antibiotics,worthy of extension at commercial scale. | Chen Chunshan Guo Minglei Wei Kai Ma Long Geng Yan | 2017 | Animal Husbandry and Feed Science2017，9，6： | 1 |
| 5 | Preparation and electrochemical lithium storage features of TiO 2 hollow spheres显示文摘 | Liang Xiao Minglei Cao Daidi Mei Yonglin Guo Lifeng Yao Deyu Qu Bohua Deng | 2013 | Journal of Power Sources2013，，： | 1 |
| 6 | Risk Assessment and Management of Electricity Markets: A Review with Suggestions显示文摘Power industry deregulation has changed the determination of electricity prices from administration approval to market clearing.Affected by multiple random factors,the market prices may fluctuate sharply or significantly increase under extreme conditions,jeopardizing the normal operation of markets.In order to effectively deal with market risks,it is essential to develop the corresponding risk-control mechanisms,especially for spot market pilots.Therefore,this paper presents an overview on the risk assessment and management schemes which are widely used in the electricity markets.First,the key issues for evaluating market risks through inherent uncertainties and market power are summarized,including analysis models and quantitative metrics.Moreover,a comprehensive review of risk management and market supervision in typical electricity markets is presented.Specifically,a multi-time risk-control framework utilized by regulators is introduced to demonstrate the available schemes to reduce market risks.In addition,the various derivative contracts and portfolio optimizations are reviewed to help market participants hedge against market risks.Finally,suggestions for the development of risk management measures in spot market pilots are proposed.The summarized experience in this paper can provide useful references and guidelines for developing risk-control mechanisms in electricity markets. | Minglei Bao Yi Ding Xiaoxin Zhou Chao Guo Changzheng Shao | 2021 | CSEE Journal of Power and Energy Systems2021，7，6： | 1 |
| 7 | Preparation and characterization of multiresponsive polymer composite microspheres with core–shell structure显示文摘 | Jie Cai Jia Guo Minglei Ji Wuli Yang Changchun Wang Shoukuan Fu | 2007 | Colloid and Polymer Science2007，，14： | 1 |
| 8 | Three-dimensional localization algorithm of wireless sensor networks base on particle swarm optimization显示文摘 | WEI Nuo GUO Qiang SHU Minglei | 2012 | Journal of China Universities of Posts and Telecommunications2012，19，2： | 1 |
| 9 | Tuner design and RF test of a four-rod RFQ显示文摘A mini-vane four-rod radio frequency quadruple (RFQ) accelerator has been built for neutron imaging. The RFQ will operate at 201.5 MHz, and its length is 2.7 m. The original electric field distribution along the electrodes is not flat. The resonant frequency needs to be tuned to the operating value. And the frequency needs to be compensated for temperature change during high power RF test and beam test. As tuning such a RFQ is difficult, plate tuners and stick tuners are designed. This paper will present the tuners design, the tuning procedure, and the RF properties of the RFQ. | QuanFeng Zhou (1) Kun Zhu (1) ZhiYu Guo (1) MingLei Kang (1) ShuLi Gao (1) YuanRong Lu (1) JiaEr Chen (1) | 2011 | Science China(Physics,Mechanics & Astronomy)2011，54，S2： | 0 |
| 10 | GroupⅢmetabotropic glutamate receptors and drug addiction显示文摘Neuroadaptations of glutamatergic transmission in the limbic reward circuitry are linked to persistent drug addiction.Accumulating data have demonstrated roles of ionotropic glutamate receptors and groupⅠandⅡmetabotropic glutamate receptors(mGluRs)in this event.Emerging evidence also identifies Gαi/o-coupled groupⅢmGluRs(mGluR4/7/8 subtypes enriched in the limbic system)as direct substrates of drugs of abuse and active regulators of drug action.Auto-and heteroreceptors of mGluR4/7/8 reside predominantly on nerve terminals of glutamatergic corticostriatal and GABAergic striatopallidal pathways,respectively.These presynaptic receptors regulate basal and/or phasic release of respective transmitters to maintain basal ganglia homeostasis.In response to operant administration of common addictive drugs,such as psychostimulants(cocaine and amphetamine),alcohol and opiates,limbic groupⅢmGluRs undergo drastic adaptations to contribute to the enduring remodeling of excitatory synapses and to usually suppress drug seeking behavior.As a result,a loss-of-function mutation(knockout)of individual groupⅢreceptor subtypes often promotes drug seeking.This review summarizes the data from recent studies on three groupⅢreceptor subtypes(mGluR4/7/8)expressed in the basal ganglia and analyzes their roles in the regulation of dopamine and glutamate signaling in the striatum and their participation in the addictive properties of three major classes of drugs(psychostimulants,alcohol,and opiates). | Limin Mao Minglei Guo Daozhong Jin Bing Xue John Q.Wang | 2013 | Frontiers of Medicine2013，7，4： | 0 |
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