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| 1 | Perspectives on studies on soil carbon stocks and the carbon sequestration potential of China显示文摘Soil carbon stocks and sequestration have been given a lot of attention recently in the study of terrestrial ecosystems and global climate change.This review focuses on the progress made on the estimation of the soil carbon stocks of China,and the characterization of carbon dynamics of croplands with regard to climate change,and addresses issues on the mineralization of soil organic carbon in relation to greenhouse gas emissions.By integrating existing research data,China's total soil organic carbon(SOC) stock is estimated to be 90 Pg and its inorganic carbon(SIC) stock as 60 Pg,with SOC sequestration rates in the range of 20-25 Tg/a for the last two decades.An estimation of the biophysical potential of SOC sequestration has been generally agreed as being 2 Pg over the long term,of which only 1/3 could be attainable using contemporary agricultural technologies in all of China's croplands.Thus,it is critical to enhance SOC sequestration and mitigate climate change to improve agricultural and land use management in China.There have been many instances where SOC accumulation may not induce an increased amount of decomposition under a warming scenario but instead favor improved cropland productivity and ecosystem functioning.Furthermore,unchanged or even decreased net global warming potential(GWP) from croplands with enhanced SOC has been reported by a number of case studies using life cycle analysis.Future studies on soil carbon stocks and the sequestration potential of China are expected to focus on:(1) Carbon stocks and the sequestration capacity of the earths' surface systems at scales ranging from the plot to the watershed and(2) multiple interface processes and the synergies between carbon sequestration and ecosystem productivity and ecosystem functioning at scales from the molecular level to agro-ecosystems.Soil carbon science in China faces new challenges and opportunities to undertake integrated research applicable to many areas. | ZHENG JuFeng CHENG Kun PAN GenXing Pete SMITH LI LianQing ZHANG XuHui ZHENG JinWei HAN XiaoJun DU YanLing | 2011 | Chinese Science Bulletin2011，56，35： | 24 |
| 2 | Numerical simulation of leading edge cavitation within the whole flow passage of a centrifugal pump显示文摘In this paper, the periodically unsteady pressure field and head-drop phenomenon caused by leading edge cavitation have been investigated numerically by computational fluid dynamics (CFD) in a single stage centrifugal pump. A CFD model for cavitation steady and unsteady simulation has been calculated using the k-ω SST turbulence model combining with a multiphase approach, based on a homogeneous model assumption. A truncated form of Rayleigh-Plesset equation is used as a source term for the inter-phase mass transfer. The CFD computational region includes the suction cone, impeller, side chambers and volute, as well as suction and pressure pipes. The results were compared with experimental data under non-cavitation and cavitation conditions and a good agreement was obtained for the global performance, the experimental data of the head and the efficiency are 34.04 m and 74.42% at BEP, respectively, the predicted head is 34.31 m and the predicted efficiency is 73.75%. The analysis of inner flow pattern shows that the vortex flow generation in the rear of cavity region is the main reason of the head-drop. Obvious increasing can be observed for the amplitude of the pressure fluctuation at the blade passing frequency with different cavitation situations, and subpeak can be found. Besides, the effects of unsteady flow in the side chambers cannot be neglected for accurately predicting the inner flow of the pump. These results imply that this numerical method is suitable for the cavitating flow in the pump. | LI XiaoJun YUAN ShouQi PAN ZhongYong YUAN JianPing FU YanXia | 2013 | Science China(Technological Sciences)2013，56，9： | 14 |
| 3 | Application of next-generation sequencing technology to precision medicine in cancer: joint consensus of the Tumor Biomarker Committee of the Chinese Society of Clinical Oncology显示文摘Next-generation sequencing(NGS) technology is capable of sequencing millions or billions of DNA molecules simultaneously.Therefore, it represents a promising tool for the analysis of molecular targets for the initial diagnosis of disease, monitoring of disease progression, and identifying the mechanism of drug resistance. On behalf of the Tumor Biomarker Committee of the Chinese Society of Clinical Oncology(CSCO) and the China Actionable Genome Consortium(CAGC), the present expert group hereby proposes advisory guidelines on clinical applications of NGS technology for the analysis of cancer driver genes for precision cancer therapy. This group comprises an assembly of laboratory cancer geneticists, clinical oncologists, bioinformaticians,pathologists, and other professionals. After multiple rounds of discussions and revisions, the expert group has reached a preliminary consensus on the need of NGS in clinical diagnosis, its regulation, and compliance standards in clinical sample collection. Moreover, it has prepared NGS criteria, the sequencing standard operation procedure(SOP), data analysis, report, and NGS platform certification and validation. | Xuchao Zhang Zhiyong Liang Shengyue Wang Shun Lu Yong Song Ying Cheng Jianming Ying Weiping Liu Yingyong Hou Yangqiu Li Yi Liu Jun Hou Xiufeng Liu Jianyong Shao Yanhong Tai Zheng Wang Li Fu Hui Li Xiaojun Zhou Hua Bai Mengzhao Wang You Lu Jinji Yang Wenzhao Zhong Qing Zhou Xuening Yang Jie Wang Cheng Huang Xiaoqing Liu Xiaoyan Zhou Shirong Zhang Hongxia Tian Yu Chen Ruibao Ren Ning Liao Chunyan Wu Zhongzheng Zhu Hongming Pan Yanhong Gu Liwei Wang Yunpeng Liu Suzhan Zhang Tianshu Liu Gong Chen Zhimin Shao Binghe Xu Qingyuan Zhang Ruihua Xu Lin Shen Yilong Wu | 2019 | Cancer Biology & Medicine2019，16，1： | 14 |
| 4 | Prognostic value of preoperative prognostic nutritional index and its associations with systemic inflammatory response markers in patients with stage Ⅲ colon cancer显示文摘Background: The prognostic nutritional index(PNI) has been widely applied for predicting survival outcomes of patients with various malignant tumors. Although a low PNI predicts poor prognosis in patients with colorectal cancer after tumor resection, the prognostic value remains unknown in patients with stage Ⅲ colon cancer undergoing curative tumor resection followed by adjuvant chemotherapy. This study aimed to investigate the prognostic value of PNI in patients with stage III colon cancer.Methods: Medical records of 274 consecutive patients with stage Ⅲ colon cancer undergoing curative tumor resection followed by adjuvant chemotherapy with oxaliplatin and capecitabine between December 2007 and December2013 were reviewed. The optimal PNI cutoff value was determined using receiver operating characteristic(ROC) curve analysis. The associations of PNI with systemic inflammatory response markers, including lymphocyte-to-monocyte ratio(LMR), neutrophil-to-lymphocyte ratio(NLR), platelet-to-lymphocyte ratio(PLR), and C-reactive protein(CRP)level, and clinicopathologic characteristics were assessed using the Chi square or Fisher's exact test. Correlation analysis was performed using Spearman's correlation coefficient. Disease-free survival(DFS) and overall survival(OS)stratified by PNI were analyzed using Kaplan-Meier method and log-rank test, and prognostic factors were identified by Cox regression analyses.Results: The preoperative PNI was positively correlated with LMR(r= 0.483, P < 0.001) and negatively correlated with NLR(r =-0.441, P < 0.001), PLR(r =-0.607, P < 0.001), and CRP level(r =-0.333, P < 0.001). A low PNI(≤49.22)was significantly associated with short OS and DFS in patients with stage ⅢC colon cancer but not in patients with stage ⅢA/ⅢB colon cancer.In addition, patients with a low PNI achieved a longer OS and DFS after being treated with6-8 cycles of adjuvant chemotherapy than did those with < 6 cycles. Multivariate analyses revealed that PNI was independently associated with DFS(hazard ratios 2.001; 95% confidence interval 1.157-3.462; P = 0.013).Conclusion: The present study identified preoperative PNI as a valuable predictor for survival outcomes in patients with stage Ⅲ colon cancer receiving curative tumor resection followed by adjuvant chemotherapy. | Jianhong Peng Rongxin Zhang Yixin Zhao Xiaojun Wu Gong Chen Desen Wan Zhenhai Lu Zhizhong Pan | 2017 | Chinese Journal of Cancer2017，36，11： | 11 |
| 5 | Ⅲ期结肠癌患者术前预后营养指数的预后价值及其与全身炎症反应标志物的相关性显示文摘背景与目的预后营养指数(prognostic nutritional index,PNI)已广泛应用于预测各种恶性肿瘤患者的生存结局。虽然低PNI预测肿瘤切除后的结直肠癌患者的预后不良,但对接受根治性肿瘤切除术后辅助化疗的Ⅲ期结肠癌患者的预后价值仍未知。本研究旨在探讨PNI对Ⅲ期结肠癌患者的预后价值。方法本文回顾了2007年12月至2013年12月期间,274例接受根治性肿瘤切除术后接受奥沙利铂和卡培他滨辅助化疗的Ⅲ期结肠癌的连续患者的医疗记录。采用受试者工作特征(receiver operating characteristic,ROC)曲线分析确定最佳PNI临界值。采用卡方检验或Fisher’s精确检验评估PNI与全身炎症反应标志物[包括淋巴细胞与单核细胞比值(lymphocyte-to-monocyte ratio,LMR)、中性粒细胞与淋巴细胞比值(neutrophil-to-lymphocyte ratio,NLR)、血小板与淋巴细胞比值(platelet-to-lymphocyte ratio,PLR)、C反应蛋白(C-reactive protein,CRP)水平]和临床病理特征的相关性。采用Spearman’s相关系数进行相关分析。采用Kaplan-Meier法和log-rank检验分析PNI分层的无病生存期(disease-free survival,DFS)和总生存期(overall survival,OS),并通过Cox回归分析确定预后因子。结果术前PNI与LMR呈正相关(r=0.483,P <0.001),与NLR(r=-0.441,P <0.001)、PLR(r=-0.607,P <0.001)和CRP水平(r=-0.333,P <0.001)呈负相关。在ⅢC期结肠癌患者中,低PNI(≤49.22)与短OS和DFS显著相关,而在ⅢA/ⅢB期结肠癌患者中则不相关。此外,在PNI低的患者中,接受6–8个疗程辅助化疗的患者比接受<6个疗程治疗的患者获得了更长的OS和DFS。多变量分析显示PNI与DFS独立相关(风险比=2.001;95%置信区间:1.157–3.462;P=0.013)。结论术前PNI是接受根治性肿瘤切除术后辅助化疗的Ⅲ期结肠癌患者生存结局的重要预测指标。 | Jianhong Peng Rongxin Zhang Yixin Zhao Xiaojun Wu Gong Chen Desen Wan Zhenhai Lu Zhizhong Pan | 2019 | 癌症2019，38，1： | 10 |
| 6 | Genetic variants in RAN, DICER and HIWI of microRNA biogenesis genes and risk of cervical carcinoma in a Chinese population显示文摘Objective:Recent evidence indicates that dysregulation of microRNA(miRNA)biogenesis is implicated in cancer development and progression.Based on the important role of miRNA biogenesis genes in carcinogenesis,we hypothesized that genetic variations of the miRNA biogenesis genes may modulate susceptibility to cervical cancer.Methods:We identified three single nucleotide polymorphisms(SNPs)located in the 3'-untranslated regions(3'-UTR)of of miRNA biogenesis key genes(rs1057035 in DICER,rs3803012 in RAN and rs10773771 in HIWI)and genotyped these SNPs in a case-control study of 1,486 cervical cancer cases and1,549 cancer-free controls in Chinese women.Results:Logistic regression analyses showed that no significant associations were observed between the three SNPs and cervical cancer risk[rs3803012 in RAN AG/GG vs.AA adjusted OR=1.104,95%confidence interval(CI):0.859-1.419;rs1057035 in DICER CT/CC vs.TT adjusted OR=0.962,95%CI:0.805-1.149;rs10773771 in HIWI CT/CC vs.TT adjusted OR=0.963,95%CI:0.826-1.122].Conclusions:The findings did not suggest that genetic variants in the 3'-UTR of RAN,DICER and HIWI of miRNA biogenesis genes were associated with the risk of cervical cancer in this Chinese population. | Jiaping Chen Zhenzhen Qin Shandong Pan Jie Jiang Li Liu Jibin Liu Xiaojun Chen Zhibin Hu Hongbing Shen | 2013 | Chinese Journal of Cancer Research2013，25，5： | 8 |
| 7 | Expression of voltage.gated sodium channel Nav1.5 in non.metastatic colon cancer and its associations with estrogen receptor(ER)-βexpression and clinical outcomes显示文摘Background: Voltage-gated sodium channel 1.5(Nav1.5) potentially promotes the migratory and invasive behaviors of colon cancer cells. Hitherto, the prognostic significance of Nav1.5 expression remains undetermined. The present study aimed to explore the associations of Nav1.5 expression with clinical outcomes and estrogen receptor-β(ER-β)expression in non-metastatic colon cancer patients receiving radical resection.Methods: A total of 269 consecutive patients with pathologically confirmed stages Ⅰ-Ⅲ colon cancer who underwent radical resection were selected. Nav1.5 and ER-β expression was detected by using immunohistochemistry(IHC)on tissue microarray constructed from paraffin-embedded specimens. IHC score was determined according to the percentage and intensity of positively stained cells. Statistical analysis was performed with the X-tile method, k coefficient, Chi square test or Fisher's exact test, logistic regression, log-rank test, and Cox proportional hazards models.Results: We found that Nav1.5 was commonly expressed in tumor tissues with higher mean IHC score as compared with matched tumor-adjacent normal tissues(5.1 ± 3.5 vs. 3.5 ± 2.7, P < 0.001).The high expression of Nav1.5 in colon cancer tissues was associated with high preoperative carcinoembryonic antigen level [odds ratio(OR) = 2.980;95% confidential interval(CI)1.163-7.632; P = 0.023] and high ER-β expression(OR = 2.808; 95% CI 1.243-6.343;p = 0.00 3). Log-rank test results showed that high Nav1.5 expression contributed to a low 5-year disease-free survival(DFS) rate in colon cancer patients(77.2% vs. 92.1%, P = 0.048), especially in patients with high ER-β expression tumor(76.2% vs. 91.3%, P = 0.032). Analysis with Cox proportional hazards model demonstrated that high Nav1.5 expression[hazard ratio(HR) = 2.738; 95% CI 1.100-6.819;P = 0.030] and lymph node metastasis(HR = 2.633; 95% CI 1.632-4.248; P < 0.001) were prognostic factors for unfavorable DFS in colon cancer patients.Conclusions: High expression of Nav1.5 was associated with high expression of ER-β and indicated unfavorable oncologic prognosis in patients with non-metastatic colon cancer. | Jianhong Peng Qingjian Ou Xiaojun Wu Rongxin Zhang Qian Zhao Wu Jiang Zhenhai Lu Desen Wan Zhizhong Pan Yujing Fang | 2017 | Chinese Journal of Cancer2017，36，12： | 7 |
| 8 | Anp32e, a higher eukaryotic histone chaperone directs preferential recognition for H2A,Z显示文摘H2A.Z 是在所有种类的高度保存的 histone 变体。H2A.Z 的染色质免职被改变复杂 SWR1 和它的哺乳动物的对应物 SRCAP 的酵母染色质明确地催化。然而, H2A.Z 被 non-histone 蛋白质优先地被认出的机制留下逃犯。这里,我们识别了 Anp32e,为 H2A.Z Anp32e 的新奇更高优核质特定的 histone 女伴优先地在 vitro 并且在 vivo 把 dimers 而非 H2A-H2B dimers 与 H2A.Z-H2B 联系并且分裂 non-nucleosomal 总数由 DNA 和 H2A-H2B 形成了。我们与 H2A.Z-H2B 在建筑群决定了 Anp32e 女伴领域(186-232 ) 的水晶结构更暗淡。在这结构,包含 Anp32e 残余 214-224 的区域,在另外的 Anp32 家庭蛋白质不在,明确地与扩大 H2A.Z α 交往; C 螺旋,哪个展览一个意外 conformational 变化。Anp32e 的染色体宽的介绍表明在 Anp32e 和 H2A.Z 房间 overexpressing Anp32e 之间的显著合作占有在 +1 nucleosomes 显示了强壮的全球 H2A.Z 损失,而房间显示的 Anp32e 弄空在 +1 nucleosomes 的中等全球 H2A.Z 增加。这建议 Anp32e 可以帮助解决 non-nucleosomal H2A.Z 总数并且也在 +1 nucleosomes,和后者便于 H2A.Z 的移动可以帮助 RNA 聚合酶 II 通过第一个 nucleosomal 障碍。 | Zhuo Mao Lu Pan Weixiang Wang Jian Sun Shah Shan Qiang Dong Xiaoping Liang Linchang Dai Xiaojun Ding She Chen Zhuqiang Zhang Bing Zhu Zheng Zhou | 2014 | Cell Research2014，24，4： | 6 |
| 9 | Identifying the molecular basis of Jinhong tablets against chronic superficial gastritis via chemical profile identification and symptom-guided network pharmacology analysis显示文摘Chronic superficial gastritis(CSG)is a common disease of the digestive system that possesses a serious pathogenesis.Jinhong tablet(JHT),a traditional Chinese medicine(TCM)prescription,exerts therapeutic effects against CSG.However,the molecular basis of its therapeutic effect has not been clarified.Herein,we employed ultra-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry(UPLC-Q/TOF-MS)based chemical profile identification to determine the chemical components in JHT.Further,we applied network pharmacology to illustrate its molecular mechanisms.A total of 96 chemical constituents were identified in JHT,31 of which were confirmed using reference standards.Based on the bioinformatics analysis using the symptom-guided pharmacological networks of“chi,”“blood,”“pain,”and“inflammation,”and target screening through the interaction probabilities between compounds and targets,matrix metalloproteinase 2(MMP2),dopamine d2 receptor(DRD2),and Aldo-keto reductase family 1 member B1(AKR1B1)were identified as key targets in the therapeutic effect exhibited by JHT against CSG.Moreover,according to the inhibitory activities presented in the literature and binding mode analysis,the structural types of alkaloids,flavonoids,organic acids,including chlorogenic acid(10),caffeic acid(13),(-)-corydalmine(33),(-)-isocorypalmine(36),isochlorogenic acid C(38),isochlorogenic acid A(41),quercetin-3-O-a-L-rhamnoside(42),isochlorogenic acid B(47),quercetin(63),and kaempferol(70)tended to show remarkable activities against CSG.Owing to the above findings,we systematically identified the chemical components of JHT and revealed its molecular mechanisms based on the symptoms associated with CSG. | Danfeng Shi Lingxian Liu Haibo Li Dabo Pan Xiaojun Yao Wei Xiao Xinsheng Yao Yang Yu | 2022 | Journal of Pharmaceutical Analysis2022，12，1： | 6 |
| 10 | Influence of temperature on the microstructure of V_2O_5 film prepared by DC magnetron sputtering显示文摘V2O5 films were prepared on silicon wafers by DC magnetron sputtering and post-annealing under various conditions. The influence of depositing and post-annealing temperatures on microstructure of V2O5 films was studied by XRD and Raman scattering measurements. The results reveal that sputtered V2O5 films show preferred growth orientation along (001) planes and the c-axis is perpendicular to the silicon substrate surface. It is interesting to find that both the V2O5 film deposited at temperature of 511 ℃ and the one annealed at 500 ℃ exhibit desirable layer-type structure of orthorhombic symmetry. Such layer-typed V2O5 films are promising candidates for cathodes of rechargeable lithium or magnesium thin-film batteries. | SU Qing PAN Xiaojun XIE Erqing WANG Yinyue QIU Jiawen LIU Xueqin | 2006 | Rare Metals2006，25，z1： | 6 |
| 11 | Total mesorectal excision with or without preoperative chemoradiotherapy for resectable mid/low rectal cancer: a long-term analysis of a prospective, single-center, randomized trial显示文摘Background:The preliminary results of our phase II randomized trial reported comparable functional sphincter pres-ervation rates and short-term survival outcomes between patients undergoing total mesorectal excision(TME)with or without preoperative concurrent chemoradiotherapy(CCRT).We now report the long-term results after a median follow-up of 71 months.Methods:Between March 23,2008 and August 2,2012,192 patients with T3-T4 or node-positive,resectable,mid/low rectal adenocarcinoma were randomly assigned to receive TME with or without preoperative CCRT.The following endpoints were assessed:cumulative rates of local recurrence and distant metastasis,disease-free survival(DFS),and overall survival(OS).Results:The data of 184 eligible patients were analyzed:94 patients in the TME group and 90 patients in the CCRT+TME group.In the whole cohort,the 5-year DFS and OS rates were 84.8%and 85.1%,respectively.The 5-year DFS rates were 85.2%in the CCRT+TME group and 84.3%in the TME group(P=0.969),and the 5-year OS rates were 83.5%in the CCRT+TME group and 86.5%in the TME group(P=0.719).The 5-year cumulative rates of local recur-rence were 6.3%and 5.0%(P=0.681),and the 5-year cumulative rates of distant metastasis were 15.0%and 15.7%(P=0.881)in the CCRT+TME and TME groups,respectively.No significant improvements in 5-year DFS and OS were observed with CCRT by subgroup analyses.Conclusions:Both treatment strategies yielded similar long-term outcomes.A selective policy towards preoperative CCRT is thus recommended for rectal cancer patients if high-quality TME surgery and enhanced chemotherapy can be performed. | Fulong Wang Wenhua Fan Jianhong Peng Zhenhai Lu Zhizhong Pan Liren Li Yuanhong Gao Hui Li Gong Chen Xiaojun Wu Peirong Ding Zhifan Zeng Desen Wan | 2018 | Cancer Communications2018，38，1： | 6 |
| 12 | Quantitative trait locus analysis of lateral branch-related traits in cucumber(Cucumis sativus L.)using recombinant inbred lines显示文摘A group of 224 recombinant inbred lines (RILs) was derived from a narrow cross between 2 cucumber (Cucumis sativus L.) lines, namely, S94 (Northern China type with weak lateral branch growth potential and early lateral branch sprouting time) and S06 (Northern European type with strong lateral branch growth potential and late lateral branch sprouting time). These lines were then used for investigating lateral branch-related traits. A total of 36 quantitative trait loci (QTLs) were detected for the following 4 lateral branch-related traits: lateral branch average length (LBAL), lateral branch total length (LBTL), lateral branch number (LBN), and first lateral branch node (FLBN). Further, each QTL explained 3.1% (lbtl2.1, spring) to 32.3% (lbn2.3, spring) of the observed phenotypic variance. Eleven QTLs (lbal1.1, lbtl1.1, lbn1.2, flbn1.2, etc.) for different traits were found to be clustered on the e23m18d-ME23EM6c section (7.4 cM) of linkage group (LG) 1; further, 15 QTLs (lbal2.1, lbtl2.1, lbn2.1, flbn2.1, etc.) were found to be clustered on the S94A1-ME4SA4a section (13.9 cM) of LG2. Twenty-one QTLs explained more than 10% of the phenotypic variance. Moreover, lbtl1.3 (autumn, 26.2%, logarithm of odds (LOD) = 17.4; spring, 26.9%, LOD = 17.9) had stable position and contribution in both seasons. Several se-quence-anchor markers (CMBR40, F, CS30, S94A1, CSWTA11B, etc.) were closely linked with some QTLs for LBAL, LBTL, LBN, and FLBN, which can be used for the marker-assisted selection to improve the plant architecture in cucumber breeding. | JIANG Su YUAN XiaoJun PAN JunSong HE HuanLe CAI Run | 2008 | Science China(Life Sciences)2008，51，9： | 5 |
| 13 | The maternally expressed polycomb group gene OsEMF2a is essential for endosperm cellularization and imprinting in rice显示文摘Cellularization is a key event in endosperm development.Polycomb group(PcG)genes,such as Fertilization-Independent Seed 2(FIS2),are vital for the syncytium-to-cellularization transition in Arabidopsis plants.In this study,we found that OsEMF2a,a rice homolog of the Arabidopsis PcG gene Embryonic Flower2(EMF2),plays a role similar to that of FIS2 in regard to seed development,although there is limited sequence similarity between the genes.Delayed cellularization was observed in osemf2a,associated with an unusual activation of type I MADS-box genes.The cell cycle was persistently activated in osemf2a caryopses,which was likely caused by cytokinin overproduction.However,the overaccumulation of auxin was not found to be associated with the delayed cellularization.As OsEMF2a is a maternally expressed gene in the endosperm,a paternally inherited functional allele was unable to recover the maternal defects of OsEMF2a.Many imprinted rice genes were deregulated in the defective hybrid seeds of osemf2a(♀)/9311(♂)(m9).The paternal expression bias of some paternally expressed genes was disrupted in m9 due to either the activation of maternal alleles or the repression of paternal alleles.These findings suggest that OsEMF2a-PRC2-mediated H3K27me3 is necessary for endosperm cellularization and genomic imprinting in rice. | Xiaojun Cheng Meiyao Pan Zhiguo E Yong Zhou Baixiao Niu Chen Chen | 2021 | Plant Communications2021，2，1： | 5 |
| 14 | Injectable collagen scaffold promotes swine myocardial infarction recovery by long-term local retention of transplanted human umbilical cord mesenchymal stem cells显示文摘Stem cell therapy is an attractive approach for recovery from myocardial infarction(MI)but faces the challenges of rapid diffusion and poor survival after transplantation.Here we developed an injectable collagen scaffold to promote the long-term retention of transplanted cells in chronic MI.Forty-five minipigs underwent left anterior descending artery(LAD)ligation and were equally divided into three groups 2 months later(collagen scaffold loading with human umbilical mesenchymal stem cell(hUMSC)group,hUMSC group,and placebo group(only phosphate-buffered saline(PBS)injection)).Immunofluorescence staining indicated that the retention of transplanted cells was promoted by the collagen scaffold.Echocardiography and cardiac magnetic resonance imaging(CMR)showed much higher left ventricular ejection fraction(LVEF)and lower infarct size percentage in the collagen/hUMSC group than in the hUMSC and placebo groups at 12 months after treatment.There were also higher densities of vWf-,α-sma-,and cTnT-positive cells in the infarct border zone in the collagen/cell group,as revealed by immunohistochemical analysis,suggesting better angiogenesis and more cardiomyocyte survival after MI.Thus,the injectable collagen scaffold was safe and effective on a large animal myocardial model,which is beneficial for constructing a favorable microenvironment for applying stem cells in clinical MI. | Qiang Wangn Xiaojun He Bin Wang Jun Pan Chunying Shi Jie Li Liudi Wang Yannan Zhao Jianwu Dai Dongjin Wang | 2021 | Science China(Life Sciences)2021，64，2： | 5 |
| 15 | Generation of 84-fs pulses from a mode-locked Tm:CNNGG disordered garnet crystal laser显示文摘A mode-locked laser based on a Tm:CNNGG disordered crystal as an active medium and a single-walled carbon nanotube saturable absorber is demonstrated, operating at a central wavelength of 2018 nm. Transformlimited 84 fs pulses are generated with an average output power of 22 mW at a repetition rate of ~90 MHz.A maximum output power of 98 mW is obtained at a slightly longer pulse duration of 114 fs. | ZHONGBEN PAN YICHENG WANG YONGGUANG ZHAO HUALEI YUAN XIAOJUN DAI HUAQIANG CAI JI EUN BAE SUN YOUNG CHOI FABIAN ROTERMUND XAVIER MATEOS JOSEP MARIA SERRES PAVEL LOIKO UWE GRIEBNER VALENTIN PETROV | 2018 | Photonics Research2018，6，8： | 4 |
| 16 | Is there a survival benefit from adjuvant chemotherapy for patients with liver oligometastases from colorectal cancer after curative resection?显示文摘Background:Although colorectal oligometastases to the liver can potentially be cured with aggressive local abla-tion,the efficacy of adjuvant chemotherapy(ACT)for such metastasis remains unclear.The present study explored the effects of ACT on patients with colorectal liver oligometastases(CLO)after curative resections and aimed to iden-tify patients who could benefit from ACT.Methods:We retrospectively analyzed 264 eligible patients with CLO who underwent curative resection between September 1999 and June 2015.Recurrence-free survival(RFS)and overall survival(OS)were analyzed using the Kaplan-Meier method and log-rank test;prognostic factors were a by Cox regression modeling.Results:Among 264 patients,200(75.8%)patients received ACT and 64(24.2%)did not receive ACT.These two groups did not significantly differ in clinicopathologic characteristics,and had comparable 3-year OS and RFS rates(RFS:42.1%vs.45.7%,P=0.588;OS:69.7%vs.62.7%,P=0.446)over a median follow-up duration of 35.5 months,irrespective of preoperative chemotherapy.ACT markedly improved 3-year OS in high-risk patients with Memorial Sloan-Kettering Cancer Center clinical risk scores(MSKCC-CRS)of 3-5(68.2%vs.33.8%,P=0.015),but presented no additional benefit in patients with MSKCC-CRS of 0-2(72.2%vs.78.6%,P=0.834).In multivariate analysis,ACT was independently associated with improved OS in patients with MSKCC-CRS of 3-5.Conclusions:ACT might offer a prognostic benefit in high-risk patients with CLOs after curative liver resection,but not in low-risk patients.Therefore,patients’risk status should be determined before ACT administration to optimize postoperative therapeutic strategies. | Zhizhong Pan Jianhong Peng Junzhong Lin Gong Chen Xiaojun Wu Zhenhai Lu Yuxiang Deng Yujie Zhao Qiaoqi Sui Desen Wan | 2018 | Cancer Communications2018，38，1： | 4 |
| 17 | Effects of biochar amendment on soil quality, crop yield and greenhouse gas emission in a Chinese rice paddy: A field study of 2 consecutive rice growing cycles显示文摘 | Afeng Zhang Rongjun Bian Genxing Pan Liqiang Cui Qaiser Hussain Lianqing Li Jinwei Zheng Jufeng Zheng Xuhui Zhang Xiaojun Han Xinyan Yu | 2011 | Field Crops Research2011，，： | 4 |
| 18 | Lipopolysaccharide induced intestinal epithelial injury:a novel organoids-based model for sepsis in vitro显示文摘Background: Advances in organoid culture technology have provided a greater understanding of disease pathogenesis, which has been rarely studied in sepsis before. We aim to establish a suitable organoids-based intestinal injury model for sepsis.Methods: Stable passaged organoids were constructed and pre-treated with lipopolysaccharide (LPS) to mimic sepsis-induced intestinal injury. The LPS-induced sepsis model was used as a reference. We used quantitative real-time polymerase chain reaction to evaluate the RNA levels of inflammatory factors and antimicrobial peptides. Enzyme-linked immunosorbent assay was used to evaluate the protein levels, hematoxylin and eosin staining was used to evaluate the pathology of the small intestine of mice, and immunohistochemistry and immunofluorescence were used to evaluate the intestinal epithelial barrier function. Perkin Elmer OperettaTM was used to obtain high-resolution images of three-dimensional organoids.Results: An LPS concentration >150 μg/mL after 24 h was identified to cause organoid growth restriction. The fluorescence intensity of zonula occludens-1 and occludins at LPS concentrations >100 μg/mL decreased significantly after 24 h. After LPS stimulation for 8 h, the RNA expression levels of interleukin (IL)-1α, tumor necrosis factor alpha, granulocyte-macrophage colony-stimulating factor, IL-6, and regenerating islet-derived protein 3 alpha, beta, and gamma increased. These results resembled those of intestinal epithelial layer alterations in a mouse sepsis model. For IL-10, the RNA expression level increased only when the LPS level >200 μg/mL for 24 h.Conclusions: This study provides the primary intestinalin vitro model to study the effects of LPS-induced intestinal injury resembling sepsis. This model provides a platform for immune associated mechanism exploration and effective drug screening. | Sisi Huang Sheng Zhang Limin Chen Xiaojun Pan Zhenliang Wen Yizhu Chen Lidi Zhang Jiao Liu Dechang Chen | 2022 | Chinese Medical Journal2022，135，18： | 3 |
| 19 | Involvement of acid-sensing ion channel 1a in gastric carcinoma cell migration and invasion显示文摘酸的微型环境,特别地酸察觉到的离子隧道 1a (ASIC1a ) ,被报导了象移植一样支持癌房间增长。在这研究,我们在胃的癌(GC ) 的移植和侵略上探索了 ASIC1a 的效果。ASIC1a 表示层次被 immunohistochemistry 从 16 个病人在配对的 GC 和邻近的正常纸巾检验。反向的抄写即时 PCR 和 immunoblotting 被进行与 ASIC1a 小发卡 RNA (shRNA ) 在 transfection 以后在 GC 房间线 AGS 估计 ASIC1a 表示层次。创伤愈合和 transwell 侵略试金被利用检测转移和侵略追随者 ASIC1a silencing。肿瘤形成被用来在 vivo 在 tumorigenicity 检测 ASIC1a 的角色。ASIC1a 表示水平在与非转移相比显示出手术后的转移的 GC 纸巾和非肿瘤纸巾是显著地更高的,这被发现。而且,有 shRNA 的 ASIC1a 的 silencing 显著地下面调整的 ASIC1a 表示和减少的 GC 房间移植和侵略。中等酸的细胞外的环境禁止了 GC 房间生存能力。而且, ASIC1a shRNA 在 vivo 引起了 tumorigenicity 的抑制。我们的学习是 attenuating 的第一份报告在 vitro 并且在由压制 ASIC1a 的 vivo 的 GC 的恶意的显型,并且建议一条新奇途径学习在 ASIC 和 GC 房间移植和侵略之间的关系。 | Xin Chen Xue Sun Zhe Wang Xiaojun Zhou Lu Xu Feng'e Li Xingding Zhang Ji'an Pan Lin Qi Haixin Qian Zhongqi Mao | 2018 | Acta Biochimica et Biophysica Sinica2018，50，5： | 3 |
| 20 | Nonlinear Dynamic Characteristics of the Vectored Thruster AUV in Complex Sea Conditions显示文摘The mobility of the vectored thruster AUV in different environment is the important premise of control system design. The new type of autonomous underwater vehicle (AUV) equipped with rudders and vectored thrusters which are combined to control the course is studied. Firstly, Euler angles representation and quaternion method are applied to establish six-DOF kinematic model respectively, then Newton second law and Lagrangian approach are used to deduce the vectored thruster AUV's nonlinear dynamic equations with six degrees of freedom (DOF) respectively in complex sea conditions based on the random wave theory according to the structural and kinetic characteristics of the vectored thruster AUV in this paper. The kinematic models and dynamic models based on different theories have the same expression and conclusion, which shows that the kinematic models and dynamic models of the vectored thruster AUV are accurate. The Runge-Kutta arithmetic is used to solve the dynamic equations, which not only can simulate the motions such as cruise and hover but also can describe the vehicle's low-frequency and high-frequency motion. The results of computation show that the mobility of the vectored thruster AUV in interference-free environment and the integrated signals including low-frequency motion signal and high-frequency motion signal in environmental disturbance accord with practical situation, which not only solve the problem of especial singularities when the pitch angle θ = ±90° but also clears up the difficulties of computation and display of the coupled nonlinear motion equations in complex sea conditions. Moreover, the high maneuverability of the vectored thruster AUV equipped with rudders and vectored thrusters is validated, which lays a foundation for the control system design. | GAO Fudong PAN Cunyun XU Xiaojun ZHANG Xiang | 2011 | Chinese Journal of Mechanical Engineering2011，24，6： | 3 |
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