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| 1 | Sequencing of Cultivated Peanut, Arachis hypogaea, Yields Insights into Genome Evolution and Oil Improvement显示文摘Cultivated peanut (Arachis hypogaea) is an allotetraploid crop planted in Asia, Africa, and America for edible oil and protein. To explore the origins and consequences of tetraploidy, we sequenced the allotetraploid A. hypogaea genome and compared it with the related diploid Arachis duranensis and Arachis ipaensis genomes. We annotated 39 888 A-subgenome genes and 41 526 B-subgenome genes in allotetraploid peanut. The A. hypogaea subgenomes have evolved asymmetrically, with the B subgenome resembling the ancestral state and the A subgenome undergoing more gene disruption, loss, conversion, and transposable element proliferation, and having reduced gene expression during seed development despite lacking genome-wide expression dominance. Genomic and transcriptomic analyses identified more than 2 500 oil metabolism-related genes and revealed that most of them show altered expression early in seed development while their expression ceases during desiccation, presenting a comprehensive map of peanut lipid biosynthesis. The availability of these genomic resources will facilitate a better understanding of the complex genome architecture, agronomically and economically important genes, and genetic improvement of peanut. | Xiaoping Chen Qing Lu Hao Liu Jianan Zhang Yanbin Hong Haofa Lan Haifen Li Jinpeng Wang Haiyan Liu Shaoxiong Li Manish K.Pandey Zhikang Zhang Guiyuan Zhou Jigao Yu Guoqiang Zhang Jiaqing Yuan Xingyu Li Shijie Wen Fanbo Meng Shanlin Yu Xiyin Wang Kadambot H.M.Siddique Zhong-Jian Liu Andrew H.Paterson Rajeev K.Varshney Xuanqiang Liang | 2019 | Molecular Plant2019，12，7： | 23 |
| 2 | Relationship between nutrients and plankton biomass in the turbidity maximum zone of the Pearl River Estuary显示文摘Nutrients,dissolved and particulate organic carbon and plankton(bacterio-,phyto-and zoo-)were compared in the turbidity maximum zone(TMZ) and adjacent areas(non-TMZ) in the Pearl River estuary.Our results showed that high levels of suspended substances had marked effect on dynamics of nutrients and plankton in the TMZ.Based on the cluster analysis of total suspended solids(TSS) concentrations,all stations were divided into two groups,TMZ with average TSS of 171 mg/L and non-TMZ of 45 mg/L.Suspended substances adsorbed PO^(3-)_4 and dissolved organic carbon,resulting in higher particulate phosphorus and organic carbon(POC)and lower PO^(3-)_4 and DOC in the TMZ,compared to the non-TMZ.However,suspended substances had limited effect on nitrogenous nutrients.Phytoplankton growth was light-limited due to high concentrations of suspended substances in the TMZ and a peak of phytoplankton abundance appeared in the non-TMZ.In contrast,the highest bacterial abundance occurred in the TMZ,which was likely partly responsible for low DOC levels.Two peaks of zooplankton abundance observed in the TMZ and non-TMZ in the Pearl River estuary were primarily supported by bacteria and phytoplankton,respectively.Our finding implied that high levels of suspended solids in the TMZ affect the trophic balance. | Zhen Shi Jie Xu Xiaoping Huang Xia Zhang Zhijian Jiang Feng Ye Ximei Liang | 2017 | Journal of Environmental Sciences2017，29，7： | 12 |
| 3 | Carbon pools and fluxes in the China Seas and adjacent oceans显示文摘The China Seas include the South China Sea, East China Sea, Yellow Sea, and Bohai Sea. Located off the Northwestern Pacific margin, covering 4700000 km^2 from tropical to northern temperate zones, and including a variety of continental margins/basins and depths, the China Seas provide typical cases for carbon budget studies. The South China Sea being a deep basin and part of the Western Pacific Warm Pool is characterized by oceanic features; the East China Sea with a wide continental shelf, enormous terrestrial discharges and open margins to the West Pacific, is featured by strong cross-shelf materials transport; the Yellow Sea is featured by the confluence of cold and warm waters; and the Bohai Sea is a shallow semiclosed gulf with strong impacts of human activities. Three large rivers, the Yangtze River, Yellow River, and Pearl River, flow into the East China Sea, the Bohai Sea, and the South China Sea, respectively. The Kuroshio Current at the outer margin of the Chinese continental shelf is one of the two major western boundary currents of the world oceans and its strength and position directly affect the regional climate of China. These characteristics make the China Seas a typical case of marginal seas to study carbon storage and fluxes. This paper systematically analyzes the literature data on the carbon pools and fluxes of the Bohai Sea,Yellow Sea, East China Sea, and South China Sea, including different interfaces(land-sea, sea-air, sediment-water, and marginal sea-open ocean) and different ecosystems(mangroves, wetland, seagrass beds, macroalgae mariculture, coral reefs, euphotic zones, and water column). Among the four seas, the Bohai Sea and South China Sea are acting as CO_2 sources, releasing about0.22 and 13.86–33.60 Tg C yr^(-1) into the atmosphere, respectively, whereas the Yellow Sea and East China Sea are acting as carbon sinks, absorbing about 1.15 and 6.92–23.30 Tg C yr^(-1) of atmospheric CO_2, respectively. Overall, if only the CO_2 exchange at the sea-air interface is considered, the Chinese marginal seas appear to be a source of atmospheric CO_2, with a net release of 6.01–9.33 Tg C yr^(-1), mainly from the inputs of rivers and adjacent oceans. The riverine dissolved inorganic carbon (DIC) input into the Bohai Sea and Yellow Sea, East China Sea, and South China Sea are 5.04, 14.60, and 40.14 Tg C yr^(-1),respectively. The DIC input from adjacent oceans is as high as 144.81 Tg C yr^(-1), significantly exceeding the carbon released from the seas to the atmosphere. In terms of output, the depositional fluxes of organic carbon in the Bohai Sea, Yellow Sea, East China Sea, and South China Sea are 2.00, 3.60, 7.40, and 5.92 Tg C yr^(-1), respectively. The fluxes of organic carbon from the East China Sea and South China Sea to the adjacent oceans are 15.25–36.70 and 43.93 Tg C yr^(-1), respectively. The annual carbon storage of mangroves, wetlands, and seagrass in Chinese coastal waters is 0.36–1.75 Tg C yr^(-1), with a dissolved organic carbon(DOC) output from seagrass beds of up to 0.59 Tg C yr^(-1). Removable organic carbon flux by Chinese macroalgae mariculture account for 0.68 Tg C yr^(-1) and the associated POC depositional and DOC releasing fluxes are 0.14 and 0.82 Tg C yr^(-1), respectively. Thus, in total, the annual output of organic carbon, which is mainly DOC, in the China Seas is 81.72–104.56 Tg C yr^(-1). The DOC efflux from the East China Sea to the adjacent oceans is 15.00–35.00 Tg C yr^(-1). The DOC efflux from the South China Sea is 31.39 Tg C yr^(-1). Although the marginal China Seas seem to be a source of atmospheric CO_2 based on the CO_2 flux at the sea-air interface, the combined effects of the riverine input in the area, oceanic input, depositional export,and microbial carbon pump(DOC conversion and output) indicate that the China Seas represent an important carbon storage area. | Nianzhi JIAO Yantao LIANG Yongyu ZHANG Jihua LIU Yao ZHANG Rui ZHANG Meixun ZHAO Minhan DAI Weidong ZHAI Kunshan GAO Jinming SONG Dongliang YUAN Chao LI Guanghui LIN Xiaoping HUANG Hongqiang YAN Limin HU Zenghu ZHANG Long WANG Chunjie CAO Yawei LUO Tingwei LUO Nannan WANG Hongyue DANG Dongxiao WANG Si ZHANG | 2018 | Science China Earth Sciences2018，61，11： | 12 |
| 4 | Holocene aeolian stratigraphic sequences in the eastern portion of the desert belt(sand seas and sandy lands) in northern China and their palaeoenvironmental implications显示文摘This paper presents the environmental history and its responses to palaeoclimatic changes since the start of the Holocene in the eastern portion of the desert belt(sand seas and sandy lands) in northern China by comparing the aeolian sandpalaeosol sequences and their palaeoclimatic proxies. The optically stimulated luminescence(OSL) ages of the aeolian sandpalaeosol sedimentary sequences and a series of palaeoenvironmental proxies show that:(1) The large-scale dune landscape currently in the Kubuqi Sand Sea was formed during the Holocene in general;and the palaeosol was generally developed during the period of 4–2 ka, indicating conditions favorable for vegetation growth, soil development, and organic carbon accumulation due to increased precipitation or effective moisture and weakened aeolian activities;the large-scale expansion of dunes in the recent 2 ka is closely linked to human activities. The variable discharge of the Yellow River with diversions for irrigation may have resulted in a more consistent supply of aeolian particles for dune field expansion.(2) The dune landscape of the Hunshandake Sandy Land was likely formed around 12 ka, and before this, the western part of the Hunshandake Sandy Land would have been covered by a single large lake;it was obviously wetter than today in the sandy land during the period of 9.6–3 ka and the palaeosols were developed at the same time. But the aeolian activities have not been completely dormant in this long-lasting wetter epoch;because the Holocene wetter period was likely time-transgressive across the region.(3) The palaeosol of the Hulunbuir Sandy Land began to develop as early as 14.5 ka, probably continuing until the last 2 ka. The palaeosol development of various dune fields in the eastern portion of the desert belt(sand seas and sandy lands) in northern China is spatially heterogeneous, and even the palaeosol development time in different locations within each sandy land is inconsistent. During the middle Holocene(especially the 7.5–3.5 ka), all the sandy lands were stabilized in general and the intensity of aeolian activities was significantly weakened. The number of palaeoenvironmental records in the eastern portion of the desert belt(sand seas and sandy lands) in northern China has increased rapidly in the past decade, but the amount of published data still does not match the vast extent of the dune fields. It does require much more in-depth palaeoenvironmental studies for a full understanding of the relationship between aeolian activities and climate change in northern China. | Xiaoping YANG Peng LIANG Deguo ZHANG Hongwei LI Patrick RIOUAL Xulong WANG Bing XU Zhibang MA Qianqian LIU Xiaozong REN Fangen HU Yuxin HE Gang RAO Ninghua CHEN | 2019 | Science China Earth Sciences2019，62，8： | 10 |
| 5 | Investigation of influence of coal properties on dense-phase pneumatic conveying at high pressure显示文摘Experiments of dense-phase pneumatic conveying of pulverized coal using nitrogen were carried out in a test facility at pressures of up to 3.7 MPa to study the effects of coal type, particle size and moisture content on flow characteristics. The Jenike shear test and scanning electron microscopy (SEM) were employed to provide a better understanding of effects of the material properties on flow characteristics. Two kinds of pulverized coals, Yanzhou and Datong, with similar particle size, moisture content and density, were used in the test. Pressure drop increases with increasing the particle size at similar solid-gas ratio, superficial velocity and pressure in the receiving hopper, and pressure drops through different test sections decrease firstly and then rise with increasing the conveying velocity for the same particle size, mass flow rate and pressure in the receiving hopper. The flowability of pulverized coal decreases with increasing the moisture content in the range from 3.24% to 8.18%. Unconfined yield strength (UYS) increases and flow function (FF) decreases with increasing the moisture content. Results of the shearing tests are consistent with the results of the conveying study. Pressure drops through different test sections are discussed and analyzed. | Cai Liang Xiaoxu Xie Pan Xu Xiaoping Chen Changsui Zhao Xin Wu | 2012 | Particuology2012，10，3： | 8 |
| 6 | MiRNA-203 suppresses tumor cell proliferation, migration and invasion by targeting Slug in gastric cancer显示文摘 | Liuqing Yang Hongwei Liang Yanbo Wang Shanting Gao Kai Yin Zhijian Liu Xi Zheng Ying Lv Lei Wang Chen-Yu Zhang Xi Chen Guifang Xu Weijie Zhang Xiaoping Zou | 2016 | Protein & Cell2016，7，5： | 7 |
| 7 | Field trial of an HTS filter system on a CDMA base station显示文摘This paper presents the field trial results of a high temperature superconductor filter system (HTSFS) in a code division multiple access (CDMA) mobile communication base station (BTS) in Tangshan, China. The center frequency of the HTSFS is 830 MHz with 1.4% fractional bandwidth. The noise figure (NF) of the HTSFS, including connectors and cables, is 0.8 dB. The field trial results show that after the HTSFS was installed in the receiver front-end of a CDMA base station, the transmitting power of handset was decreased by 3.1 dB on average. Up to now, the HTSFS has been running steadily in the base station for more than 22 months, demonstrating the reliability and stability of the system. | YIN ZheSheng WEI Bin CAO BiSong WANG Xin GUO XuBo ZHANG XiaoPing GAO LongMa PIAO YunLong ZHU MaFeng LIANG Yong WANG Fan H. PIEL B. AMINOV F. AMINOVA M. GETTA S. KOLESOV A. KNACK N. PUPETER D. WEHLER | 2007 | Chinese Science Bulletin2007，52，2： | 7 |
| 8 | Anp32e, a higher eukaryotic histone chaperone directs preferential recognition for H2A,Z显示文摘H2A.Z 是在所有种类的高度保存的 histone 变体。H2A.Z 的染色质免职被改变复杂 SWR1 和它的哺乳动物的对应物 SRCAP 的酵母染色质明确地催化。然而, H2A.Z 被 non-histone 蛋白质优先地被认出的机制留下逃犯。这里,我们识别了 Anp32e,为 H2A.Z Anp32e 的新奇更高优核质特定的 histone 女伴优先地在 vitro 并且在 vivo 把 dimers 而非 H2A-H2B dimers 与 H2A.Z-H2B 联系并且分裂 non-nucleosomal 总数由 DNA 和 H2A-H2B 形成了。我们与 H2A.Z-H2B 在建筑群决定了 Anp32e 女伴领域(186-232 ) 的水晶结构更暗淡。在这结构,包含 Anp32e 残余 214-224 的区域,在另外的 Anp32 家庭蛋白质不在,明确地与扩大 H2A.Z α 交往; C 螺旋,哪个展览一个意外 conformational 变化。Anp32e 的染色体宽的介绍表明在 Anp32e 和 H2A.Z 房间 overexpressing Anp32e 之间的显著合作占有在 +1 nucleosomes 显示了强壮的全球 H2A.Z 损失,而房间显示的 Anp32e 弄空在 +1 nucleosomes 的中等全球 H2A.Z 增加。这建议 Anp32e 可以帮助解决 non-nucleosomal H2A.Z 总数并且也在 +1 nucleosomes,和后者便于 H2A.Z 的移动可以帮助 RNA 聚合酶 II 通过第一个 nucleosomal 障碍。 | Zhuo Mao Lu Pan Weixiang Wang Jian Sun Shah Shan Qiang Dong Xiaoping Liang Linchang Dai Xiaojun Ding She Chen Zhuqiang Zhang Bing Zhu Zheng Zhou | 2014 | Cell Research2014，24，4： | 6 |
| 9 | Superelastic wire-shaped supercapacitor sustaining 850% tensile strain based on carbon nanotube@graphene fiber显示文摘 | Huimin Wang Chunya Wang Muqiang Jian Qi Wang Kailun Xia Zhe Yin Mingchao Zhang Xiaoping Liang Yingying Zhang | 2018 | Nano Research2018，11，5： | 6 |
| 10 | Effects of Tillage Management on Infiltration and Preferential Flow in a Black Soil, Northeast China显示文摘The impacts of no-tillage (NT) and moldboard plough (MP) managements on infiltration rate and preferential flow were characterized using a combined technique of double-ring device and dye tracer on a black soil (Mollisols) in Northeast China. The objective of this study is to evaluate how tillage practices enhance soil water infiltration and preferential flow in favor of soil erosion control in the study area. The steady infiltration rates under NT management are 1.6 and 2.1 times as high as those under MP management in the 6th and 8th years of the tillage management in place, while the infiltrated water amounts under NT management are 1.4 and 2.0 times as high as those under MP management, respectively. The depth of methylene blue penetrated into NT soil increases from 43cm in the 6th year to 57cm in the 8th year, which are 16cm and 19cm deeper than those in MP soil, respectively. The results of morphologic image show that more biological macro-pores occur in NT soil than in MP soil. These macro-pores play a key role in enhancing preferential flow in NT soil, which in turn promotes water infiltration through preferential pathways in NT soil. The results are helpful to policy-making in popularizing NT and have the implications for tillage management in regard to soil erosion control in black soil region of China. | FAN Ruqin ZHANG Xiaoping YANG Xueming LIANG Aizhen JIA Shuxia CHEN Xuewen | 2013 | Chinese Geographical Science2013，23，3： | 6 |
| 11 | Physical sensors for skin-inspired electronics显示文摘Skin,the largest organ in the human body,is sensitive to external stimuli.In recent years,an increasing number of skin-inspired electronics,including wearable electronics,implantable electronics,and electronic skin,have been developed because of their broad applications in healthcare and robotics.Physical sensors are one of the key building blocks of skin-inspired electronics.Typical physical sensors include mechanical sensors,temperature sensors,humidity sensors,electrophysiological sensors,and so on.In this review,we systematically review the latest advances of skin-inspired mechanical sensors,temperature sensors,and humidity sensors.The working mechanisms,key materials,device structures,and performance of various physical sensors are summarized and discussed in detail.Their applications in health monitoring,human disease diagnosis and treatment,and intelligent robots are reviewed.In addition,several novel properties of skin-inspired physical sensors such as versatility,self-healability,and implantability are introduced.Finally,the existing challenges and future perspectives of physical sensors for practical applications are discussed and proposed. | Shuo Li Yong Zhang Yiliang Wang Kailun Xia Zhe Yin Huimin Wang Mingchao Zhang Xiaoping Liang Haojie Lu Mengjia Zhu Haomin Wang Xinyi Shen Yingying Zhang | 2020 | InfoMat2020，2，1： | 5 |
| 12 | An 84-month observational study of the changes in CD4 T-lymphocyte cell count of 110 HIV/AIDS patients treated with traditional Chinese medicine显示文摘这研究试图由与病毒(HIV )/acquired 免疫不全症候群(爱滋病) 与 TCM 连续地对待 84 个月的人的免疫不全在 110 个盒子的 CD4 T 淋巴细胞房间计数观察变化评估繁体中文药(TCM ) 的治疗学的效果。从从 2004 ~ 2013 与 TCM 对待的 19 个省和城市的 110 个 HIV/AIDS 病人的信息是镇定的。在 CD4 计数的索引的变化( 200 , 201350 , 351500 并且> 500 房间/公里<啜class=“ a-plus-plus ”>在五次的 3 )指( 0 , 12 , 36 , 60 和 84 个月)并且不同治疗[加 antiretroviral 治疗(艺术)的 TCM 和 TCM ]被比较。重复措施测试没显示在组和时间之间的相互作用(P > 0.05 ) 。在四个不同框架增加和二个组的减少的 CD4 计数的学位与基线相比是统计上重要的。在二个组之间的 CD4 计数不是统计上重要的。为 200 房间 / 公里的 CD4 计数 < 啜 class= “ a-plus-plus ” > 吝啬的 CD4 计数改变的 3 , 是 21 和 28 房间 / 公里 < 啜 class= “ a-plus-plus ” > 每为 TCM 的年,加艺术的组和 TCM 分别地组织的 3 。为 201350 房间 / 公里的 CD4 计数 < 啜 class= “ a-plus-plus ” > 吝啬的 CD4 计数改变的 3 , 是 6 和 25 房间 / 公里 < 啜 class= “ a-plus-plus ” > 每为 TCM 的年,加艺术的组和 TCM 分别地组织的 3 。为 351500 房间 / 公里的 CD4 计数 < 啜 class= “ a-plus-plus ” > 吝啬的 CD4 计数改变的 3 , 是 13 和 7 房间 / 公里 < 啜 class= “ a-plus-plus ” > 每为 TCM 的年,加艺术的组和 TCM 分别地组织的 3 。为 CD4 计数 > 500 房间 / 公里 < 啜 class= “ a-plus-plus ” > 吝啬的 CD4 计数改变的 3 , 是 34 和 17 房间 / 公里 < 啜 class= “ a-plus-plus ” > 每为 TCM 的年,加艺术的组和 TCM 分别地组织的 3 。TCM 的长期的使用能与 HIV/AIDS 在病人维持或变慢,并且可以完成持续有效性衰退 CD4 计数的步。 | Jian Wang Biyan Liang Xiaoping Zhang Liran Xu Xin Deng Xiuhui Li Lu Fang Xinghua Tan Yuxiang Mao Guoliang Zhang Yuguang Wang | 2014 | Frontiers of Medicine2014，8，3： | 5 |
| 13 | IDSRadar:a real-time visualization framework for IDS alerts显示文摘Intrusion Detection Systems(IDS) is an automated cyber security monitoring system to sense malicious activities.Unfortunately,IDS often generates both a considerable number of alerts and false positives in IDS logs.Information visualization allows users to discover and analyze large amounts of information through visual exploration and interaction efficiently.Even with the aid of visualization,identifying the attack patterns and recognizing the false positives from a great number of alerts are still challenges.In this paper,a novel visualization framework,IDSRadar,is proposed for IDS alerts,which can monitor the network and perceive the overall view of the security situation by using radial graph in real-time.IDSRadar utilizes five categories of entropy functions to quantitatively analyze the irregular behavioral patterns,and synthesizes interactions,filtering and drill-down to detect the potential intrusions.In conclusion,IDSRadar is used to analyze the mini-challenges of the VAST challenge 2011 and 2012. | ZHAO Ying ZHOU FangFang FAN XiaoPing LIANG Xing LIU YongGang | 2013 | Science China(Information Sciences)2013，56，8： | 5 |
| 14 | Thrombolysis with alteplase 3-4.5 hours after acute ischaemic stroke:the first multicentre,phase III trial in China显示文摘background and purpose Data on the efficacy and safety of alteplase for acute ischaemic stroke(AIS)administered 3-4.5 hours after the onset of stroke symptoms in Chinese patients is limited.We sought to determine whether AIS patients would benefit from thrombolysis with alteplase between 3 and 4.5 hours after the onset of stroke symptoms in a prospective,multicentre,single-arm trial in China.Materials and methods Eligible AIS patients were given 0.9 mg/kg alteplase intravenously.The primary efficacy endpoint was a favourable outcome at 3 months,defined as a score of 0 or 1 on the modified Rankin Scale.Thresholds for the primary efficacy endpoint were determined to be 40%based on the literature review.The primary safety endpoint was symptomatic intracranial haemorrhage(sICH)according to the European Cooperative Acute Stroke Study III(ECASS III)trial definition.Post hoc analysis between this study and the ECASS III trial were compared using the propensity score matching(PSM)method.results A total of 120 eligible AIS patients from 11 sites in China received thrombolysis therapy in this study.The median time from onset of symptoms to needle was 3 hours 54 min.The percentage of patients with a favourable outcome was 63.3%(95%CI 54.4 to 71.4),significantly higher than the predefined threshold(p<0.0001).Three patients(2.5%,95%CI 0.5 to 7.1)had sICH,including two fatal sICH.Six patients died within 3 months after treatment.The post hoc PSM analysis showed a numerically higher rate of the primary efficacy endpoint in this study(63.3%)than the matched placebo arm(56.7%)in the ECASS III trial.Conclusions Intravenous alteplase with a standard dose administered between 3 and 4.5 hours after onset of symptoms is effective and safe for Chinese AIS patients. | Huaguang Zheng Yi Yang Huisheng Chen Chuanling Li Yangkun Chen Fu-Dong Shi Li Yang Xiaoping Cui Zuneng Lu Yanling Liang Songbiao Cui Anding Xu Yiqing Wu Yaqing Sun Yongjun Wang | 2020 | Stroke & Vascular Neurology2020，5，3： | 4 |
| 15 | Sub-micrometer-scale chemical analysis by nanosecond-laser-induced tip-enhanced ablation and ionization time-of-flight mass spectrometry显示文摘The tip-enhanced near-field technique has drawn great attention recently becauseit is a promising technique for nanoscale chemical analysis when combined withother spectroscopic methods. Furthermore, this integration can improve the spatialresolution of mass spectrometry. In this stud~ a nanosecond-laser (nsqaser)-inducedtip-enhanced ablation and ionization source was coupled to an in-house-builtlaser ionization time-of-flight mass spectrometer. Sub-micrometer-sized craters(with diameters of 200-300 nm) were observed on titanium (Ti) film coated ontoa gold (Au) substrate. The corresponding mass spectra were acquired, whichverified that sub-micrometer-sized ablation (and subsequently ionization) couldbe achieved by the ns-laser-induced tip-enhanced electromagnetic field. Inaddition, formation of the crater was studied with an increased number of laserpulses. Furthermore, a mass spectrometry imaging (MSI) experiment wasperformed with potassium chloride (KC1) residue dropped onto nano-patternedgold pillars, achieving 80-nm lateral resolution. | Xiaoping Li Zhisen Liang Shudi Zhang Tongtong Wang Wei Hang | 2018 | Nano Research2018，11，11： | 4 |
| 16 | Fargeted elimination of mutant mitochondria DNA in MELAS-iPSCs by mitoTALENs显示文摘 | Yi Yang Han Wu Xiangjin Kang Yanhui Liang Ting Lan Tianjie Li Tao Tan Jiangyun Peng Quanjun Zhang Geng An Yali Liu Qian Yu Zhenglai Ma Ying Lian Boon Seng Soh Qingfeng Chen Ping Liu Yaoyong Chen Xiaofang Sun Rong Li Xiumei Zhen Yang Yu Xiaoping Li Yong Fan | 2018 | Protein & Cell2018，9，3： | 4 |
| 17 | Resection of Giant Hepatic Cavernous Hemangiomas after Dissection of the Third Porta Hepatis显示文摘Objective:To estabhsh a novel and safe operation technique for the resection of giant hepatic cavernous hemangiomas involving the retro-hepatic vena cava.Methods:After ligating the hepatic artery of affected lobe, the short hepatic veins at the third porta hepatis were dissected and ligated individually to separate the tumor from the retrohepatic vena cava, followed by the resection of the tumor under intermittent interruption of the porta hepatis.Results:A total of 62 giant hepatic cavernous hemangiomas were successfully resected without hepatic vascular exclusion. Right and caudate lobectomies were done in 27 cases, right hemihepatectornies in 5 cases, right upper segnentectomies in 7 cases, right posterior lobectomies in 7 cases, extended left and caudate lobectomies in 10 cases, and caudate lobectomies in 6 cases. The blood transfusion requirement during operation was 1 400 ml on average. All did well postoperatively during a follow up of 4 - 84 months.Conclusion:It is safe and feasible to resect giant hepatic cavernous hemangioma following dissection of the third porta hepatis. Duringoperation the key step is dissection of the short hepatic veins. | YAO Xiaoping, ZHOU Weiping, WANG Yi, WU Mengchao, JING Liang Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai 200438, China | 2002 | The Chinese-German Journal of Clinical Oncology2002，1，1： | 4 |
| 18 | Chinese expert consensus on conversion therapy for hepatocellular carcinoma(2021 edition)显示文摘Recent advances in systemic and locoregional treatments for patients with unresectable or advanced hepatocellular carcinoma(HCC)have resulted in improved response rates.This has provided an opportunity for selected patients with initially unresectable HCC to achieve adequate tumor downstaging to undergo surgical resection,a‘conversion therapy’strategy.However,conversion therapy is a new approach to the treatment of HCC and its practice and treatment protocols are still being developed.Review the evidence for conversion therapy in HCC and develop consensus statements to guide clinical practice.Evidence review:Many research centers in China have accumulated significant experience implementing HCC conversion therapy.Preliminary findings and data have shown that conversion therapy represents an important strategy to maximize the survival of selected patients with intermediate stage to advanced HCC;however,there are still many urgent clinical and scientific challenges for this therapeutic strategy and its related fields.In order to summarize and learn from past experience and review current challenges,the Chinese Expert Consensus on Conversion Therapy for Hepatocellular Carcinoma(2021 Edition)was developed based on a review of preliminary experience and clinical data from Chinese and non-Chinese studies in this field and combined with recommendations for clinical practice.Sixteen consensus statements on the implementation of conversion therapy for HCC were developed.The statements generated in this review are based on a review of clinical evidence and real clinical experience and will help guide future progress in conversion therapy for patients with HCC. | Hui-Chuan Sun Jian Zhou Zheng Wang Xiufeng Liu Qing Xie Weidong Jia Ming Zhao Xinyu Bi Gong Li Xueli Bai Yuan Ji Li Xu Xiao-Dong Zhu Dousheng Bai Yajin Chen Yongjun Chen Chaoliu Dai Rongping Guo Wenzhi Guo Chunyi Hao Tao Huang Zhiyong Huang Deyu Li Gang Li Tao Li Xiangcheng Li Guangming Li Xiao Liang Jingfeng Liu Fubao Liu Shichun Lu Zheng Lu Weifu Lv Yilei Mao Guoliang Shao Yinghong Shi Tianqiang Song Guang Tan Yunqiang Tang Kaishan Tao Chidan Wan Guangyi Wang Lu Wang Shunxiang Wang Tianfu Wen Baocai Xing Bangde Xiang Sheng Yan Dinghua Yang Guowen Yin Tao Yin Zhenyu Yin Zhengping Yu Bixiang Zhang Jialin Zhang Shuijun Zhang Ti Zhang Yamin Zhang Yubao Zhang Aibin Zhang Haitao Zhao Ledu Zhou Wu Zhang Zhenyu Zhu Shukui Qin Feng Shen Xiujun Cai Gaojun Teng Jianqiang Cai Minshan Chen Qiang Li Lianxin Liu Weilin Wang Tingbo Liang Jiahong Dong Xiaoping Chen Xuehao Wang Shusen Zheng Jia Fan 无 | 2022 | Hepatobiliary Surgery and Nutrition2022，11，2： | 4 |
| 19 | Modified methods for culturing myoblasts of rats: Combination of multi-enzymatic digestion and double purification显示文摘BACKGROUND: With developments of tissue engineering and genetic engineering, we aim to culture myoblasts, which are characterized by high purity, high quality and high production, for wide application in neural regeneration researches. OBJECTIVE: To modify traditional dissociation method in order to obtain myoblasts, which are characterized by high purity, high quality and high production, and explore the biological properties under in vitro culture. DESIGN: Observational study. SETTING: Basic Institute of Academy of Military Medical Sciences of Chinese PLA. MATERIALS: Four neonatal Wistar rats of 5 days old, both genders and mean body mass of 10 g were selected in this study. The main reagents and devices were detailed as follows: DMEM medium (Gibco Company), fetus bovine serum (FBS, Hycolne Company), collagenase Ⅱ(Sigma Company), trypsin (Sigma Company), dispase Ⅱ (Sigma Company), desmin antibody (Fuzhou Maixin Company), antibody Ⅱ and ABC kit (Wuhan Boster Biotechnology Company), desk centrifuge (KUBATO, Japan), and inverted phase contrast microscope (LEICA DMIRB, Germany). METHODS: The experiment was carried out in the Basic Institute of Academy of Military Medical Sciences of Chinese PLA from June to October 2006. Neonatal rats were sacrificed under sterile condition to obtain skeletal muscles of limbs, which were washed with cold PBS (containing benzylpenicillin and estreptomicina), and muscular tissue was sheared into pieces. Then, those muscular pieces were added with mixed digestive enzyme (containing 2 g/L collagenase Ⅱ + 5 g/L dispase Ⅱ + 0.28 g/L CaCl2) as twice volume as pieces, dealt with mechanical pipetting for 5 minutes and cultured in CO2 incubator for 10 minutes. The operation was done for three times and the muscular pieces were digested for 45 minutes in total. Moreover, cells were suspended again in order to obtain myoblasts from skeletal muscle of neonatal rats. In addition, myoblasts were purified with differential attachment technique and enzyme digestion so as to observe morphological characteristics and growth, draw growth curve, analyze surface structure under scanning electron microscope, and evaluate with Desmin immunohistochemical staining. MAIN OUTCOME MEASURES: Morphological characteristics and growth of myoblasts cultured in vitro. RESULTS: ① Growth of myoblasts of skeletal muscle: Primary cells had well growth, mature and differentiation. The positive rate of Desmin was 94% and purification of cells was ideal. Growth curve of cells demonstrated that myoblasts which were characterized by high purification started proliferation plentiful through transient growth lag phase (about at one or two days after inoculation). If myoblasts were not dealt with any interventions, they might become sarcotubule gradually at 3–5 days after proliferative phase. During this period, myoblasts maintained a monocaryon-bipolarity state under inverted phase contrast microscope. Furthermore, the growth of cells was the strongest and reproductive activity was the most powerful. This suggested that myotube started to form; in addition, muscle fiber of contractility might form under a well culturing condition. ② Immunocytochemical stain with desmin antibody: Interzonal fiber of desmin from myoblasts showed strongly positive reaction. Positive staining existed in cytoplasm had a high nucleus-cytoplasm ratio. However, myoblasts showed negative or mildly positive reaction. CONCLUSION: It is ideal for modified multi-enzymatic digestion and double purification method to dissociate and purify myoblasts of skeletal muscle; meanwhile, these two methods are both the effective ways to provide convenient conditions to obtain seed cells for neural regeneration researches. | Li Zhang Wei Wang Ming Fan Xiaoping Chen Shuhong Liu Liang Sun | 2007 | Neural Regeneration Research2007，2，1： | 3 |
| 20 | Effect of puerarin on myocardial perfusion and ventricular wall motion in patients with acute coronary syndrome显示文摘Objective To investigate the effects of puerarin (Pur) on myocardial perfusion and ventricular wall motion in patients with acute coronary syndrome (ACS).Methods Thirty-seven patients with ACS were randomly divided into two groups:conventional treatment group (n= 17,11 males,range of age:32-80 years,average age:60.9±4.9 years) and Pur treatment group (n=20,12 males,range of age:40-76 years,average age:62.7±3.5 years).Patients in the conventional treatment group received standard treatment according to the current guidelines,while patients in the Pur treatment group received intravenous administration of Pur (500 mg/day) for 10 days plus conventional treatment.Real-time myocardial contrast echocardiography (RT-MCE) was performed to evaluate the change in myocardial perfusion index (MPI) and ventricular wall motion index (VWMI) at admission and 10 days after treatment.Results At 10 days after treatment,MPI was significantly higher (P<0.01) and VWMI significantly lower (P<0.01) in the Pur group comparing with those in the conventional group.Conclusions Puerarin might improve myocardial microcirculation perfusion and ventricular wall motion in patients with ACS. | Ling Niu Dongye Li Yong Xia Defeng Pan Xiaoping Wang Yan Yan Li Liang Tongda Xu Cardiovascular Institute of Xuzhou Medical College, Department of Cardiology, Affiliated Hospital, Xuzhou Medical College, Xuzhou, Jiangsu 221002, China | 2008 | Journal of Geriatric Cardiology2008，5，3： | 3 |
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