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| 1 | IL-17 response mediates acute lung injury induced by the 2009 Pandemic Influenza A (HIN1) Virus显示文摘 | Chenggang Li Penghui Yang Yang Sun Taisheng Li Chen Wang Zhong Wang Zhen Zou Yiwu Yan Wei Wang Chen Wang Zhongwei Chen Li Xing Chong Tang Xiangwu Ju Feng Guo Jiejie Deng Yan Zhao Peng Yang Jun Tang Huanling Wang Zhongpeng Zhao Zhinan Yin Bin Cao Xiliang Wang Chengyu Jiang | 2012 | Cell Research2012，22，3： | 22 |
| 2 | Identification of immunoreactive proteins of Brucella melitensis by immunoproteomics显示文摘Infection with Brucella causes brucellosis, a chronic disease in humans, which induces abortion and sterility in livestock. Among the different Brucella species, Brucella melitensis is considered the most virulent and is the predominant species associated with outbreaks in China. To date, no safe human vaccine is available against Brucella infection. The currently used live vaccines against Brucella in livestock induce antibodies that interfere with the diagnosis of field infection in vaccinated animals, which is harmful to eradication programs. However, there is as yet no complete profile of immunogenic proteins of B. melitensis. Towards the development of a safer, equally efficacious, and field infection-distinguishable vaccine, we used immunoproteomics to identify novel candidate immunogenic proteins from B. melitensis M5. Eighty-eight immunoreactive protein spots from B. melitensis M5 were identified by Western blotting and were assigned to sixty-one proteins by mass spectrometry, including many new immunoreactive proteins such as elongation factor G, F0F1 ATP synthase subunit beta, and OMP1. These provide many candidate immunoreactive proteins for vaccine development. | ZHAO ZhongPeng YAN Fang JI WenHui LUO DeYan LIU Xin XING Li DUAN YueQiang YANG PengHui SHI XiuMin LI Zhong WANG XiLiang | 2011 | Science China(Life Sciences)2011，54，9： | 12 |
| 3 | Basic fibroblast growth factor protects against influenza A virus-induced acute lung injury by recruiting neutrophils显示文摘Influenza virus (IAV)infection is a major cause of severe respiratory illness that affects almost every country in the world.IAV infections result in respiratory illness and even acute lung injury and death,but the underlying mechanisms responsible for IAV pathogenesis have not yet been fully elucidated.In this study,the basic fibroblast growth factor 2 (FGF2)level was markedly increased in H1N1 virus-infected humans and mice.FGF2,which is predominately derived from epithelial cells,recruits and activates neutrophils via the FGFR2-PI3K-AKT-NFKB signaling pathway.FGF2 depletion or knockout exacerbated influenzaassociated disease by impairing neutrophil recruitment and activation.More importantly,administration of the recombinant FGF2 protein significantly aUeviated the severity of IAV-induced lung injury and promoted the survival of IAV-infected mice.Based on the results from experiments in which neutrophils were depleted and adoptively transferred,FGF2 protected mice against IAV , infection by recruiting neutrophils.Thus,FGF2 plays a critical role in preventing IAV-induced lung injury,and FGF2 is a promising potential therapeutic target during IAV infection. | Keyu Wang Chengcai Lai Tieling Li Cheng Wang Wei Wang Bing Ni Changqing Bai Shaogeng Zhang Lina Han Hongjing Gu Zhongpeng Zhao Yueqiang Duan Xiaolan Yang Li Xing Lingna Zhao Shanshan Zhou Min Xia Chengyu Jiang Xiliang Wang Penghui Yang | 2018 | Journal of Molecular Cell Biology2018，10，6： | 3 |
| 4 | Response of BALB/c mice to a monovalent influenza A (H1N1) 2009 split vaccine显示文摘新奇流行性感冒 A (H1N1 ) 2009 病毒出现了到原因第一第二十一个世纪流行。流行性感冒 A (H1N1 ) 病毒引起的疾病爆发关于潜力推动了担心为一流行并且对流行性感冒 A 的这种子类型驾驶了疫苗的发展。在这研究,我们开发了一支一价的流行性感冒 A (H1N1 ) 裂口疫苗并且在 BALB/c 老鼠评估了它的效果。老鼠与包含红血球凝聚素的疫苗的 2 剂量皮下地被使免疫(哈) 独自一个或哈加铝氢氧化物(艾尔(哦) 3 ) 助手。有改变剂量的免疫哈(3.75, 7.5, 15, 30, 45 或 60 渭 g ) 被执行导致抵销抗体的生产。疫苗的得到的强壮的红血球凝聚抑制(嗨) 并且助手的 microneutralization,和增加扩充了抗体反应。初步的安全评估证明疫苗不是有毒的逍遥法外剂量(包含 60 渭 g HA+600 渭 g 艾尔的 0.5 ml (哦) 3 或 60 渭 g 哈) 。而且,疫苗被发现在 120 渭 g HA+1200 渭 g 艾尔的剂量安全(哦) 3 或 120 渭 g 哈在在老鼠的 1.0 ml。在结论,现在的学习作为公共健康干预为流行性感冒 A (H1N1 ) 种痘的临床的评估提供支持减轻一可能流行。另外,我们疫苗的进一步的评估在首领或人在这研究使用了的调查结果支持。 | Penghui Yang Li Xing Chong Tang Weihong Jia Zhongpeng Zhao Kun Liu Xiao Gao Xiliang Wang | 2010 | Cellular & Molecular Immunology2010，7，2： | 2 |
| 5 | Enhanced Influenza VLP vaccines comprising matrix-2 ectodomain and nucleoprotein epitopes protects mice from lethal challenge显示文摘 | Xiao Gao WenJuan Wang YuFeng Li ShaoGeng Zhang YueQiang Duan Li Xing ZhongPeng Zhao PeiRui Zhang ZhiWei Li RuiSheng Li Xiliang Wang PengHui Yang | 2013 | Antiviral Research2013，，1： | 1 |
| 6 | Virus-like particle vaccine by intranasal vaccination elicits protective immunity against respiratory syncytial viral infection in mice显示文摘呼吸 syncytial 病毒(RSV ) 是在婴儿和孩子的更低的呼吸感染的一个领先的原因,但是仍然没有可得到的准许的疫苗。在这份报告,我们基于 Bac-to-Bac baculovirus 表达式系统开发了像病毒的粒子(VLP ) 疫苗,由一个流行性感冒病毒矩阵(M1 ) 组成蛋白质和 RSV 熔化蛋白质(F) 或 glycoprotein (G) 。这些 RSV VLP 被西方的污点分析和电子显微镜学识别。有 RSV-F VLP, RSV-G VLP,或两个的 intranasally 使免疫的女 BALB/c 老鼠(i.n ) 对 RSV 显示出病毒特定的抗体回答。全部的 IgG, IgG1, IgG2a,和 mucosal IgA 与 RSV-F 正 RSV-G VLP 在老鼠被检测,揭示细胞的有势力和 mucosal 免疫者回答。而且,我们发现这些混合 RSV VLP 对实时 RSV 挑战授与提高的保护,在病毒的复制和组织病理学说的变化的明显的变细与病毒的感染联系了的肺的出现重要减少。这些结果证明由 intranasal 种痘的 RSV-F 正 RSV-G VLP 是保证使用棉花老鼠和首领模型的进一步的评估的一个有希望的疫苗的候选人。 | Mengying Cai Cheng Wang Yufeng Li Hongjing Gu Sujing Sun Yueqiang Duan Chengcai Lai Keyu Wang Xiaolan Yang Li Xing Peirui Zhang Zhaohai Wang Shaogeng Zhang Xiaodong Guo Shubing Liu Yigang Tong Xiliang Wang Penghui Yang | 2017 | Acta Biochimica et Biophysica Sinica2017，49，1： | 1 |
| 7 | Influenza virus vaccine expressing fusion and attachment protein epitopes of respiratory syncytial virus induces protective antibodies in BALB/c mice显示文摘 | Chengrong Bian Shuzhen Liu Na Liu Guangzhou Zhang Li Xing Yingwei Song Yueqiang Duan Hongjing Gu Ya Zhou Peirui Zhang Zhiwei Li Keming Zhang Zhaohai Wang Shaogeng Zhang Xiliang Wang Penghui Yang | 2014 | Antiviral Research2014，，： | 1 |
| 8 | Formation mechanism of high-quality reservoirs in intermediate volcanic rocks:A case study of Longfengshan area in Songliao Basin显示文摘The basin margin slope area of Changling fault depression in Songliao Basin is dominated by intermediate-basic volcanic rocks of Huoshiling Formation,and there is still a lack of in-depth understanding of the development pattern and formation mechanism of high-quality reservoirs in intermediate-basic volcanic rocks in this area.Taking the intermediate volcanic rocks in Longfengshan area of Changling fault depression as an example,a comprehensive study was undertaken via core observations,thin section porosity analysis,rock physical property tests,scanning electron microscopy and X-ray diffraction analysis,combined with well log and seismic d ata.As a result,formation mechanism of a high quality reservoir was made clear based on dissolution modification effects of the volcanic rocks,and the development model of the high quality reservoir under t he dual control of lithology and fluid was established.The research shows that the volcanic rocks in this area consist of six lithologies:andesitic hydrothermal breccia,andesite,andesitic volcanic breccia,andesitic tuff,dacitic tuff and sedimentary tuff,which are controlled by structure,eruptive source,eruptive action and palaeotopography.The proximal facies of the volcanic edifice were located on the basin margin,distributed along faults mainly consisting of overflow facies intermixed with eruptive facies,and gradually transformed into thick eruptive facies(middle-source facies)and volcanic sedimentary facies(distal facies).The reservoir space of volcanic rocks was dominated by dissolution pores,which were formed by weathering and dissolution by organic acidic fluids.The thermal debris flow subphase in the middle-source facies of the volcanic edifice had a large amount of easily dissolved volcanic ash,which was close to the source rock in the centre of the basin and was a favourable area for the development of dissolution pores.This study is providing a guide for oil and gas exploration in the intermediate-basic volcanic rocks in the slope of Changling fault depression in Songliao Basin. | XING Jian SHAN Xuanlong REN Xianjun YI Jian LIU Chaoyang NIU Penghui | 2022 | Global Geology2022，25，2： | 1 |
| 9 | N6-methyladenosine modification of CENPF mRNA facilitates gastric cancer metastasis via regulating FAK nuclear export显示文摘Background:N6-methyladenosine(m^(6)A)modification is the most common modification that occurs in eukaryotes.Although substantial effort has been made in the prevention and treatment of gastric cancer(GC)in recent years,the prognosis of GC patients remains unsatisfactory.The regulatory mechanism between m^(6)A modification and GC development needs to be elucidated.In this study,we examined m^(6)A modification and the downstream mechanism in GC.Methods:Dot blotting assays,The Cancer Genome Atlas analysis,and quantitative real‑time PCR(qRT-PCR)were used to measure the m^(6)A levels in GC tissues.Methylated RNA-immunoprecipitation sequencing and RNA sequencingwere performed to identify the targets ofm^(6)Amodification.Western blotting,Transwell,wound healing,and angiogenesis assays were conducted to examine the role of centromere protein F(CENPF)in GC in vitro.Xenograft,immunohistochemistry,and in vivo metastasis experiments were conducted to examine the role of CENPF in GC in vivo.Methylated RNA-immunoprecipitation-qPCR,RNA immunoprecipitation-qPCR and RNA pulldown assays were used to verify the m^(6)A modification sites of CENPF.Gain/loss-of-function and rescue experiments were conducted to determine the relationship between CENPF and the mitogen-activated protein kinase(MAPK)signaling pathway in GC cells.Coimmunoprecipitation,mass spectrometry,qRT-PCR,and immunofluorescence assays were performed to explore the proteins that interact with CENPF and elucidate the regulatory mechanisms between them.Results:CENPF was upregulated in GC and facilitated the metastasis of GC both in vitro and in vivo.Mechanistically,increasedm^(6)A modification of CENPF was mediated by methyltransferase 3,and this modified molecule could be recognized by heterogeneous nuclear ribonucleoprotein A2/B1(HNRNPA2B1),thereby promoting its mRNA stability.In addition,the metastatic phenotype of CENPF was dependent on the MAPK signaling pathway.Furthermore,CENPF could bind to FAK and promote its localization in the cytoplasm.Moreover,we discovered that high expression of CENPF was related to lymphatic invasion and overall survival in GC patients.Conclusions:Our findings revealed that increased m^(6)A modification of CENPF facilitates the metastasis and angiogenesis of GC through the CENPF/FAK/MAPK and epithelial-mesenchymal transition axis.CENPF expression was correlated with the clinical features of GC patients;therefore,CENPF may serve as a prognostic marker of GC. | Penghui Xu Jing Yang Zetian Chen Xing Zhang Yiwen Xia Sen Wang Weizhi Wang Zekuan Xu | 2023 | Cancer Communications2023，43，6： | 0 |
| 10 | The 2009 pandemic A/Wenshan/01/2009 H1N1 induces apoptotic cell death in human airway epithelial cells显示文摘In 2009,a novel swine-origin H1N1 influenza virus emerged in Mexico and quickly spread to other countries,including China.This 2009 pandemic H1N1 can cause human respiratory disease,but its pathogenesis remains poorly understood.Here,we studied the infection and pathogenesis of a new 2009 pandemic strain,A/Wenshan/01/2009 H1N1,in China in human airway epithelial cell lines compared with contemporary seasonal H1N1 influenza virus.Our results showed that viral infection by the A/Wenshan H1N1 induced significant apoptotic cell death in both the human nasopharyngeal carcinoma cell line CNE-2Z and the human lung adenocarcinoma cell line A549.The A/Wenshan H1N1 virus enters both of these cell types more efficiently than the seasonal influenza virus.Viral entry in both cell lines was shown to be mediated by clathrin-and dynamin-dependent endocytosis.Therefore,we discovered that the 2009 pandemic H1N1 strain,A/Wenshan/01/2009,can induce apoptotic cell death in epithelial cells of the human respiratory tract,suggesting a molecular pathogenesis for the 2009 pandemic H1N1. | Ning Yang Xiaoxu Hong Penghui Yang Xiangwu Ju Yuguo Wang Jun Tang Chenggang Li Quanshui Fan Fuqiang Zhang Zhongwei Chen Li Xing Zhongpeng Zhao Xiao Gao Guoyang Liao Qihan Li Xiliang Wang Dangsheng Li Chengyu Jiang | 2011 | Journal of Molecular Cell Biology2011，3，4： | 0 |
| 11 | Regulatory effect of vascular endothelial growth factor on blood spinal cord barrier in presyrinx state of experimental syringomyelia显示文摘BACKGROUND:Vascular endothelial growth factor (VEGF) is able to regulate blood spinal cord barrier function as well as influence neovascularization and cause edema. OBJECTIVE: Through establishment of a rabbit model of syringomyelia,to explore the correlation between VEGF protein and mRNA expressions and function of blood spinal cord barrier and edema degree of spinal cord in presyrinx state. DESIGN,TIME AND SETTING: Randomized controlled animal study was performed in the Tumor Institute of the Fourth Hospital,Hebei Medical University from January to June 2007. MATERIALS: A total of 0.6 mL Kaolin solution (250 g/L,37 ℃) was injected into the cisterna magna of 40 rabbits in the kaolin group to establish syringomyelia models. Goat anti-rabbit VEGF monoclonal antibody was provided by DIACLONE Company,USA; RT-PCR related reagents were provided by Huamei Bioengineering Co.,Ltd.,Beijing. METHODS: Sixty Chinese white rabbits were divided randomly into two groups: Kaolin group (n=40) and control group (n=20). Physiological saline (0.6 mL at 37 ℃) was injected in rabbits of control group. On days 1,3,7,14 and 21 after kaolin injection,cervical cords samples were harvested after sacrifice of animal. MAIN OUTCOME MEASURES: VEGF protein and mRNA expressions were detected by immunohistochemistry and RT-PCR on days 1,3,7,14,and 21 after kaolin injection. A quantitative analysis of blood spinal cord barrier function was performed by Evans blue technique. Water content of the spinal cord was measured by dry-wet weight technique. The correlation between the expression of VEGF protein and mRNA and the function of blood spinal cord barrier in the upper cervical cord of the presyrinx state was analyzed by linear correlation analysis. RESULTS: The water content and Evans blue content increased in the kaolin group on days 1 and 3 postoperatively compared with the control group (F=7.387,61.35,P<0.05,0.01),and reached a peak on day 7 (F=135.94,528.35,P<0.01). They declined slowly to day 21 postoperatively,but both contents were still higher than the control group (F=11.51,58.63,P<0.01). VEGF protein expression increased on day 1,and stronger positive expression was seen on days 3,7 and 14. It decreased on day 21. VEGF protein expression was higher than the control group at each time point (F=137.4-468.5,P<0.01). VEGF mRNA expression showed the same pattern in the cervical cord at different time points. By statistical analysis,the expression of VEGF protein and mRNA had a significantly positive correlation with the structural and functional changes of the blood spinal cord barrier in the presyrinx state (r=0.604-0.979,P<0.05). CONCLUSION: In the presyrinx state of syringomyelia,the expressions of VEGF protein and mRNA can influence the structure and function of the blood spinal cord barrier and play an important role in the formation and development of spinal cord edema and syringomyelia. | Jianfeng Li Changrong Zhou Haiying Liu Penghui Xing | 2008 | Neural Regeneration Research2008，3，11： | 0 |
| 12 | Microscopic pore characteristics of andesite and implication from mineral content:A case study in Huoshiling Formation of Changling fault depression,Songliao Basin显示文摘In order to study the microscopic pore characteristics of andesite reservoir and the effect of mineral content on the andesite pore,this study takes the andesite of the Huoshiling Formation in Longfengshan of Changling fault depression,Songliao Basin as a subject.The andesite reservoir space was discerned through the observation of cores and casting thin sections.Besides,the pore size distribution of andesites and their mineral contents were quantitatively characterized by high-pressure mercury injection,nitrogen adsorption and XRD,respectively.The results show that:(1)There are various types of reservoir space in andesites,including vesicles,amygdule,intergranular pores,matrix dissolution pores and dissolution pores of amygdala,and three types of fractures including dissolution,structural and explosion fractures.(2)The pore size distribution of andesite is complex.The main pore size of andesite is mid-pore(10-20 nm)with some large-pores(>50 nm).Mid-pore and large-pore provide the main specific surface area,which are the main space for gas storage.(3)The andesite reservoir space in the study area is mainly controlled by dissolution,as supported by the relationship between the change of mineral content and porosity evolution.The porosity of andesites decreases with the increase of quartz and chlorite content,but increases with the increase of soluble mineral,e.g.,feldspar content. | NIU Penghui SHAN Xuanlong REN Xianjun YI Jian LIU Chaoyang XING Jian | 2022 | Global Geology2022，25，2： | 0 |
| 13 | Study on Treating Glioblastoma with (125)~I Seed Implantation during Operations显示文摘OBJECTIVE To evaluate the therapeutic efficacy of treating glioblastoma with interstitial irradiation using radioactive seeds. METHODS First,the tumor was removed as completely as possible under an operating microscope.Then 125I seeds which had been selected were implanted in the tumor bed followed by routine radiotherapy after the operation.The time of progression(TTP)and overall survival(OS)of the patients were recorded,and statistical y compared with patients who had received only surgery and radiotherapy for glioblastoma during the same period. RESULTS Nineteen patients,who were diagnosed with glioblastoma for the first time,with a mean age of 51.2 years,were involved in this study. Radioactive seeds(8~20)were implanted into the tumor bed after tumorectomy with a mean surrounding therapeutic dose of 31.6 Gy,fol owed by an external irradiation dose of 42~54 Gy.The average TTP in the group with implanted seeds was 42.1±2.4 w(95%confidence interval,37.2~46.5 w),and 27.1±2.7 w(95%confidence interval,24.3~31.8 w)in the control group.The average OS was 66.3±3.2 w(95%confidence interval,61.9~70.4 w)and 47.7±2.6 w(95%confidence interval,44.4~50.5 w),respectively.Statistical analysis showed there was a significant difference between two groups. CONCLUSION The survival time of patients with glioblastoma can be prolonged through radioactive seed implantation at the time of operation. This therapeutic method can rapidly reduce the tumor volume with a minimum number of seeds to avoid excessive radiation exposure.Because of some shortcomings of this study,such as a smal number of patients,and because implantation was performed under the naked eye so that the seed alignment could not be verified accurately,the experience of combining implantation of seeds with external irradiation needs to be investigated further. | Yingzi Liu Jianfeng Li Xuexin Zhang LeiZhang Penghui Xing | 2007 | Chinese Journal of Clinical Oncology2007，4，6： | 0 |
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