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| 1 | Prevalence and evolution of drug resistance HIV-1 variants in Henan, China显示文摘To understand the prevalence and evolution of drug resistant HIV strains in Henan China after the implementation of free antiretroviral therapy for AIDS patients. 45 drug nave AIDS patients, 118 AIDS patients who received three months antiretroviral therapy and 124 AIDS patients who received six months antiretroviral treatment were recruited in the southern part of Henan province. Information on general condition, antiretroviral medicines, adherence and clinical syndromes were collected by face to face interview. Meanwhile, 14ml EDTA anticoagulant blood was drawn. CD4/CD8 T cell count, viral load and genotypic drug resistance were tested. The rates of clinical improvement were 55.1% and 50.8% respectively three months and six months after antiretroviral therapy. The mean CD4 cell count after antiretroviral therapy was significantly higher than in drug nave patients. The prevalence rate of drug resistant HIV strains were 13. 9%, 45.4% and 62.7% in drug nave patients, three month treatment patients and six month treatment patients, respectively. The number of resistance mutation codons and the frequency of mutations increased significantly with continued antiretroviral therapy. The mutation sites were primarily at the 103, 106 and 215 codons in the three-month treatment group and they increased to 15 codon mutations in the six-month treatment group. From this result, the evolution of drug resistant strains was inferred to begin with the high level NNRTI resistant strain, and then develop low level resistant strains to NRTIs. The HIV strains with high level resistance to NVP and low level resistance to AZT and DDI were highly prevalent because of the AZT+DDI+NVP combination therapy. These HIV strains were also cross resistant to DLV, EFV, DDC and D4T. Poor adherence to therapy was believed to be the main reason for the emergence and prevalence of drug resistant HIV strains. The prevalence of drug resistant HIV strains was increased with the continu- ation of antiretroviral therapy in the southern part of Henan province. Measures, that could promote high level adherence, provide new drugs and change ART regimens in failing patients, should be implemented as soon as possible. | Jing Yun LI Han Ping LI Lin LI Hong LI Zhe WANG Kun YANG Zuo Yi BAO Dao Min ZHUANG Si Yang LIU Yong Jian LIU Hui XING Yi Ming SHAO | 2005 | Cell Research2005，15，11： | 34 |
| 2 | Microwave Exposure Impairs Synaptic Plasticity in the Rat Hippocampus and PC12 Cells through Over-activation of the NMDA Receptor Signaling Pathway显示文摘Objective The aim of this study is to investigate whether microwave exposure would affect the N-methyl-D-aspartate receptor(NMDAR) signaling pathway to establish whether this plays a role in synaptic plasticity impairment. Methods 48 male Wistar rats were exposed to 30 m W/cm2 microwave for 10 min every other day for three times. Hippocampal structure was observed through H&E staining and transmission electron microscope. PC12 cells were exposed to 30 m W/cm2 microwave for 5 min and the synapse morphology was visualized with scanning electron microscope and atomic force microscope. The release of amino acid neurotransmitters and calcium influx were detected. The expressions of several key NMDAR signaling molecules were evaluated. Results Microwave exposure caused injury in rat hippocampal structure and PC12 cells, especially the structure and quantity of synapses. The ratio of glutamic acid and gamma-aminobutyric acid neurotransmitters was increased and the intracellular calcium level was elevated in PC12 cells. A significant change in NMDAR subunits(NR1, NR2 A, and NR2B) and related signaling molecules(Ca2+/calmodulin-dependent kinase II gamma and phosphorylated c AMP-response element binding protein) were examined. Conclusion 30 m W/cm2 microwave exposure resulted in alterations of synaptic structure, amino acid neurotransmitter release and calcium influx. NMDAR signaling molecules were closely associated with impaired synaptic plasticity. | XIONG Lu SUN Cheng Feng ZHANG Jing GAO Ya Bing WANG Li Feng ZUO Hong Yan WANG Shui Ming ZHOU Hong Mei XU Xin Ping DONG Ji YAO Bin Wei ZHAO Li PENG Rui Yun | 2015 | Biomedical and Environmental Sciences2015，28，1： | 16 |
| 3 | Influence of FasL gene expression on hepatic metastasis of colorectal carcinoma显示文摘BACKGROUND: FasL expression was reported to be asso- ciated with hepatic metastasis of colorectal cancer. The aim of this study was to study FasL gene expression in colorectal carcinoma and its influences on biological behavior and he- patic metastasis of colorectal carcinoma. METHODS: FasL gene expressions were examined with re- verse transcriptase-polymerase chain reaction (RT-PCR) in the primary focus of colorectal carcinoma, adjacent can- cerous mucosae, and metastasized liver focus from colorec- tal cancer. HR-8348 cells of human rectal cancer cell line were transfected with FasL cDNA. Cell growth suppression rate and response to 5-FU and carboplatin were observed and analyzed with the MTT method. RESULTS: FasL gene expression was detected in the prima- ry focus of colorectal cancer ( n = 58), adjacent cancerous mucosae ( n = 58), and metastasized hepatic tumor tissues (n =28). The positive rate of FasL expression was 24% (14/ 58), 8% (5/58), and 100% (58/58) in the primary focus, adjacent cancerous mucosae and metastasized hepatic tumor tissues respectively. FasL expression rate in the me- tastasized hepatic tumor tissues was higher than that in the primary focus (χ2 = 43.49, P<0. 01) and adjacent cance- rous mucosae (χ2=57.66, P<0.01). In a group of patients with hepatic metastasis, the FasL expression rate in primary focus was higher than that in patients without hepatic me- tastasis (χ2=3.96, P <0.05). In vitro study positive expres- sion of FasL was shown in transfected HR-8348 cells. When 5-FU or carboplatin was added, there was a significant difference in growth suppression rate between FasL positive and controlled cancer cells (t=9.02, t = 11.93, P<0.01). Under the same concentration of chemotherapeutic agents, the survival rate of FasL positive HR-8348 cells was higher than that of controlled cells. CONCLUSIONS: FasL positive cancer cells are powerfullyresistant to chemotherapeutic agents. The expression of the FasL gene in colorectal cancer cells is related to immune evasion to escape from being killed by immune cells, show- ing stronger drug-resistance, and it facilitates hepatic me- tastasis. | Shi-Yong Li, Bo Yu, Ping An, Jia-Chen Wei, Fu-Yi Zuo and Hui-Yun Cai Beijing, China Department of General Surgery, Beijing Military Commend General Hospital, Beijing 100700, China | 2004 | Hepatobiliary & Pancreatic Diseases International2004，3，2： | 14 |
| 4 | Prognostic evaluation of postoperative adjuvant therapy for operable cervical cancer:10 years'experience of National Cancer Center in China显示文摘Objective: The aim of this study was to investigate the prognostic factors and to evaluate the impact of adjuvant therapy on clinical outcome for early-stage cervical cancer.Methods: The clinical-pathological data of all 1,335 patients with the International Federation of Gynecology and Obstetrics(FIGO) Ib–IIa cervical cancer treated with primary radical surgery at the Chinese National Cancer Center between May 2007 and Dec 2013 were retrospectively reviewed.The median follow-up was 70 months.Results: Of all the patients,61.6% of the cases received adjuvant therapy,with 5-year disease-free survival(DFS)of 92.1% and 5-year overall survival(OS) of 95.0%.In multivariate analysis,differentiation of G3(P<0.05),lymph node metastasis(LNM,P<0.05) and lymphovascular space invasion(LVSI,P<0.05) were independent predictors for OS,while LNM(P<0.05),deep stroma invasion(DSI,P<0.05) and LVSI(P<0.05) were independent factors for DFS.The samples were stratified by histologic type,and cervical squamous cell carcinoma(SCC) was found to share the same independent factors except for differentiation of OS.As to patients with cervical adenocarcinoma/adenosquamous carcinoma(AC/ASC),differentiation was the independent predictor of OS(P<0.05); and LVSI of DFS(P<0.05).Of 236 patients with high-risk factors,there was no significant difference in survival between concurrent chemoradiotherapy(CCRT,n=195),radiotherapy(RT,n=24),and chemotherapy(CT,n=17).Among the 190 patients with LNM who underwent CCRT,124 cases showed improved DFS after sequential CT(P=0.118),with a recurrence rate decrease of 14%,though the difference was not statistically significant.Patients with single intermediate-risk factors like DSI or LVSI were found to partially benefit from adjuvant therapy,but the difference was not statistically significant.Conclusions: LNM,LVSI,DSI and differentiation were found to be independent prognostic factors for operable cervical cancer.Aggressive postoperative adjuvant therapy based on single risk factors in Chinese National Cancer Center could benefit survival.CCRT+CT outperformed CCRT in high-risk patients.For patients with single non-high-risk factor,the role of adjuvant therapy needs to be further discussed. | Tong Shu Dan Zhao Bin Li Yating Wang Shuanghuan Liu Pingping Li Jing Zuo Ping Ba i RongZhang Lingying Wu | 2017 | Chinese Journal of Cancer Research2017，29，6： | 12 |
| 5 | Two New O-terpenoidal Coumarins, Excavacoumarin A and B from Clausena excavata显示文摘Two new O-terpenoidal coumarins named excavacoumarin A (2). B (3). and a known one 1 were isolated from the leaves of Clausena excavata Burm. f: (Rutaceae) collected in Xishuangbanna, Yunnan. Structure elucidation and unambiguous NMR assignments for the title compounds were carried out on the basis of 1D and 2D NMR experiments. | Hong Ping HE Yue Mao SHEN Guo Ying ZUO Wei Ming ZHU Xiao Sheng YANG Xiao Jiang HAO (Laboratory of Phytochemistry, Kunming Institute of Botany, Chinese Academy of Sciences.Kunming, Yunnan 650204) | 2000 | Chinese Chemical Letters2000，11，6： | 6 |
| 6 | A double-blind, double-dummy, randomized controlled, multicenter trial of ^(99)Tc-methylene diphosphonate in patients with moderate to severe rheumatoid arthritis显示文摘Background:Clinical observational studies revealed that ^(99)Tc-methylene diphosphonate(^(99)Tc-MDP)could reduce joint pain and swollenness in rheumatoid arthritis(RA)patients.This multicenter,randomized,double-blind,double-dummy study aimed to evaluate the effects of ^(99)Tc-MDP plus methotrexate(MTX)vs.MTX alone or ^(99)Tc-MDP alone on disease activity and structural damage in MTX-naïve Chinese patients with moderate to severe RA.Methods:Eligible patients with moderate to severely active RA were randomized to receive ^(99)Tc-MDP plus MTX(n=59)vs.MTX(n=59)alone or ^(99)Tc-MDP(n=59)alone for 48 weeks from six study sites across four provinces in China.The primary outcomes were the American College of Rheumatology 20%improvement(ACR20)response rates at week 24 and changes in modified total Sharp score at week 48.Results:At week 24,the proportion of participants achieving ACR20 was significantly higher in the MTX+^(99)Tc-MDP combination group(69.5%)than that in the MTX group(50.8%)or ^(99)Tc-MDP group(47.5%)(P=0.03 for MTX+^(99)Tc-MDP vs.MTX,and MTX+^(99)Tc-MDP vs.^(99)Tc-MDP,respectively).The participants in the MTX+^(99)Tc-MDP group and the ^(99)Tc-MDP group had significantly less important radiographic progression than the participants in the MTX group over the 48 weeks(MTX+^(99)Tc-MDP vs.MTX:P=0.03,^(99)Tc-MDP vs.MTX:P=0.03,respectively).There was no significant difference in terms of adverse events(AEs)among the groups.No serious AEs were observed.Conclusions:This study demonstrated that the combination of ^(99)Tc-MDP with MTX inhibited structural damage and improved disease activity in RA patients compared with MTX and ^(99)Tc-MDP monotherapies,without increasing the rate of AEs.Additional clinical studies of ^(99)Tc-MDP therapy in patients with RA are warranted.Trial Registration:Chictr.org,ChiCTR-IPR-14005684;http://gffzz62a03ee25e6f4a77hvq5o5qucvb9n66nw.ffgz.tsg.suse.edu.cn/showproj.aspx?proj=10088. | Qiong Fu Ping Feng Ling-Yun Sun Xiao-Xia Zuo Dong-Bao Zhao Dong-Yi He Hua-Xiang Wu Wei Zhang Wei Zhang Fang Du Chun-De Bao | 2021 | Chinese Medical Journal2021，，12： | 5 |
| 7 | Proteomic analysis of differentially expressed proteins involving in liver metastasis of human colorectal carcinoma显示文摘BACKGROUND:Early diagnosis of liver metastasis of colorectal carcinoma is very important for the appropriate treatment of such patients.However,there has been no effective approach available for clinical application.The present study aimed to investigate the differential expression of proteins in patients with liver metastasis of colorectal carcinomas using proteomic analysis and evaluate its potentiality in clinical diagnosis. METHODS:Fluorescence two-dimensional differential in-gel electrophoresis(2-D DIGE)was used to analyze and compare the protein expression between normal mucosa,the primary focus,and liver metastases.Proteomic analysis was made to identify the differentially expressed proteins.Immunohistological staining was used to confirm the expression of differentially expressed proteins in colorectal carcinomas and areas of liver metastasis. RESULTS:A 1.5-fold difference was found with 46 differentially expressed proteins.In 20 differentially expressed proteins,3 were down-regulated and 17 up-regulated in liver metastases. Proteomic analysis showed that the S-adenosylmethionine transgelin variant was down-regulated in liver metastasis tissues.Zinc finger protein 64 homolog(Zfp64),guanine nucleotide exchange factor 4(GEF4),human arginase,glutathione S-transferases(GSTs)A3,and tumor necrosis factorα(TNF-α)-induced protein 9 were up-regulated in liver metastasis tissues.Immunohistochemical staining confirmed that human arginase expression was higher in liver metastases than in the primary focus. CONCLUSIONS:There was a significant difference in protein expression between the primary focus of colorectal carcinoma and liver metastases.The differentially regulated proteins were closely related to liver metastasis of colorectal carcinoma. Elevated human arginase may be an important molecular marker for liver metastasis from colorectal carcinoma. | Li, Shi-Yong An, Ping Cai, Hui-Yun Bai, Xue Zhang, Ying-Nan Yu, Bo Zuo, Fu-Yi Chen, Gang | 2010 | Hepatobiliary & Pancreatic Diseases International2010，9，2： | 4 |
| 8 | Distribution characteristics of heavy metals in surface sediments and core sediments of the Shenzhen Bay in Guangdong Province,China显示文摘The concentration of zinc,chromium,cadmium,nickel,lead and copper was measured on both surface sediments and core sediments in the Shenzhen Bay.The average contents of all metals have exceeded the background values in the coastal zone of Guangdong Province.Cadmium is a heavy pollution matter which indicates a strong pollution level according to the ecological risk coefficient method.The ecological risk index of the whole area is 299 which belongs to middle and strong level.The area with strong pollution level is the near shore area closing to the inside of Shenzhen Bay,Shakou area is in the middle level while the weak pollution level occurs in Shenzhenhe Estuary according to ecological risk index.The four sediment cores can be divided into two parts based on lead-210 dating,sediments size analysis and economic development period in surrounding areas since 1950 with strong human intervention.The sediment size,deposition rate and human activities have positive relationship with the contents of heavy metals which directly impact the pollution level of heavy metals in sediments in Shenzhen Bay. | ZUO Ping WANG Yaping MIN Fengyang CHENG Jun | 2009 | Acta Oceanologica Sinica2009，28，6： | 4 |
| 9 | Mechanism of the Rpn13-induced activation of Uch37显示文摘 | Lianying Jiao Songying Ouyang Neil Shaw Gaojie Song Yingang Feng Fengfeng Niu Weicheng Qiu Hongtao Zhu Li-Wei Hung Xiaobing Zuo V. Eleonora Shtykova Ping Zhu Yu-Hui Dong Ruxiang Xu Zhi-Jie Liu | 2014 | Protein & Cell2014，5，8： | 4 |
| 10 | Complex strengthening mechanisms in the NbMoTaW multiprincipal element alloy显示文摘Refractory multi-principal element alloys(MPEAs)have exceptional mechanical properties,including high strength-to-weight ratio and fracture toughness,at high temperatures.Here we elucidate the complex interplay between segregation,short-range order,and strengthening in the NbMoTaW MPEA through atomistic simulations with a highly accurate machine learning interatomic potential.In the single crystal MPEA,we find greatly reduced anisotropy in the critically resolved shear stress between screw and edge dislocations compared to the elemental metals.In the polycrystalline MPEA,we demonstrate that thermodynamically driven Nb segregation to the grain boundaries(GBs)and W enrichment within the grains intensifies the observed short-range order(SRO).The increased GB stability due to Nb enrichment reduces the von Mises strain,resulting in higher strength than a random solid solution MPEA.These results highlight the need to simultaneously tune GB composition and bulk SRO to tailor the mechanical properties of MPEAs. | Xiang-Guo Li Chi Chen Hui Zheng Yunxing Zuo Shyue Ping Ong | 2020 | npj Computational Materials2020，，1： | 4 |
| 11 | New ceramides from the flower of Albizia iulibrissin显示文摘A new ceramide and its glycoside were isolated from the flower of Albizia julibrissin. Their structures were established as (25,35,4R,8E)-2-[(2’R)-hydroxyhexadecanoylamino]-8-tetra-cosene-l,3,4-triol(I)and 1-O-β-D-glucopyranosy1-(2S,3S,4R,8E)-2-[(2’R)-hydroxy-hexade-canoylamino]-8-tetracosene-1,3,4-triol (II) on the basis of chemical and spectroscopic studies. | Jian Kang, Chang Hong Huo, Zhe Li, Zuo Ping Li Department of Natural Medicine Chemistry, Hebei Medical University, Shijiazhuang 050017, China | 2007 | Chinese Chemical Letters2007，18，2： | 4 |
| 12 | New type flooding systems in enhanced oil recovery显示文摘Wormlike 微粒,面对钠磷酸盐在离子的表面活化剂钠油酸盐(NaOA ) 获得了答案(Na 3 PO 4), 用稳定、动态的变阻器被学习逻辑方法。充满实验的实验室模拟被用来调查为象微粒系统一样的蠕虫泛滥的效果。结果证明油恢复是 32.7% 。这个泛滥的系统是一种新类型并且与低费用举办高活动。 | Xin Ping Li Li Yu Yong Qiang Ji Bo Wu Gan Zuo Li Li Qiang Zheng | 2009 | Chinese Chemical Letters2009，20，10： | 4 |
| 13 | Inhibiting Hv1 channel in peripheral sensory neurons attenuates chronic inflammatory pain and opioid side effects显示文摘Both opioids and nonsteroidal anti-inflammatory drugs(NSAIDS)produce deleterious side effects and fail to provide sustained relief in patients with chronic inflammatory pain.Peripheral neuroinflammation(PN)is critical for initiation and development of inflammatory pain.A better understanding of molecular mechanisms underlying PN would facilitate the discovery of new analgesic targets and the development of new therapeutics.Emerging evidence suggests that peripheral sensory neurons are not only responders to painful stimuli,but are also actively engaged in inflammation and immunity,whereas the intrinsic regulatory mechanism is poorly understood.Here we report the expression of proton-selective ion channel Hv1 in peripheral sensory neurons in rodents and humans,which was previously shown as selectively expressed in microglia in mammalian central nervous system.Neuronal Hv1 was up-regulated by PN or depolarizing stimulation,which in turn aggravates inflammation and nociception.Inhibiting neuronal Hv1 genetically or by a newly discovered selective inhibitor YHV98-4 reduced intracellular alkalization and ROS production in inflammatory pain,mitigated the imbalance in downstream SHP-1-pAKT signaling,and also diminished pro-inflammatory chemokine release to alleviate nociception and morphine-induced hyperalgesia and tolerance.Thus,our data reveal neuronal Hv1 as a novel target in analgesia strategy and managing opioids-related side effects. | Qiansen Zhang Yimin Ren Yiqing Mo Peipei Guo Ping Liao Yuncheng Luo Jie Mu Zhuo Chen Yang Zhang Ya Li Linghui Yang Daqing Liao Jie Fu Juwen Shen Wei Huang Xuewen Xu Yanyan Guo Lianghe Mei Yunxia Zuo Jin Liu Huaiyu Yang Ruotian Jiang | 2022 | Cell Research2022，32，5： | 3 |
| 14 | Mechanism of magnetic field-modulated luminescence from lanthanide ions in inorganic crystal:a review显示文摘The luminescence from lanthanide ions has potential applications in light emitting diodes,biomedical,solar cells,sensors,display,etc.However,the luminescence is suffered from the various problems,such as low luminescence efficiency and inharmonious wavelength for energy transfer.Magnetic field is an efficient method to modulate the wavelength and intensity of luminescence from lanthanide ions.Magnetic field redistributes the populated electrons in the excited states to tune the wavelength of lanthanide ions by Zeeman effect,mixing effect,and quantum confinement effect.Magnetic field enhances or suppresses the luminescence intensity by the administration of cross-relaxation,energy transfer,and Boltzmann population.In this review,we first introduce the various phenomena and mechanisms of magnetic field modulated downshift luminescence from lanthanide ions,including Zeeman effect,cross-relaxation,crystal structure,absorption,quantum confinement effect,and magneticoptical hysteresis.Then,we explain the regulation of upconversion luminescence by magnetic field,containing energy transfer and mixing effect.Finally,different options regarding how to understand the mechanism of magnetic field-modulated luminescence from lanthanide ions in the future are outlined. | Shan-Ling Zuo Ping Chen Cao-Feng Pan | 2020 | Rare Metals2020，39，10： | 3 |
| 15 | Characteristics and distribution patterns of reef complexes on the carbonate platform margin in deep water areas:the western South China Sea显示文摘As a potential oil and gas reservoir,reef complexes have been a research focus from petroleum geologists for a long time.There are favorable conditions for the development of reef complexes in the South China Sea; however,their internal structures,evolution and distribution are still poorly understood.Based on 2D and 3D seismic data,the internal structures and evolution patterns of the reef complexes on the carbonate platform margin in the deep water areas over the western South China Sea were studied in detail.The result shows that two types of reef complexes,i.e.,fault controlling platform margin reef complexes and ramp reef complexes have been developed in the study area.The reef complexes have independent or continuous mound or lenticular seismic reflections,with three internal structures(i.e.,aggrading,prograding and retrograding structures).There are different growth rates during the evolution of the reef complexes,resulting in the formation of catch-up reefs,keep-up reefs and quick step reefs.The study also reveals that different platform margin reef complexes have different internal structures and distributions,because of the different platform types.These results may be applied to the exploration and prediction of carbonate platform margin reef complexes in other areas that are similar to the study area. | CHEN Ping LI Xushen WANG Yahui LU Yongchao ZHONG Zehong CHEN Lei ZUO Qianmei MA Yiquan WANG Chao DU Xuebin | 2015 | Acta Oceanologica Sinica2015，34，10： | 3 |
| 16 | Association of telomere length with authentic pluripotency of ES/iPS cells显示文摘Telomerase 和 telomeres 为干细胞的不定的复制是重要的。最近,体的房间的 telomeres 被发现是 reprogrammed 在导致的 pluripotent 干细胞(iPSCs ) 伸长。然而,在在胚胎的干细胞(转换字符) 或 iPSCs 的 vivo 的发展 pluripotency 的 telomeres 的角色直接没被探讨。我们证明有长 telomeres 的转换字符展出真发展 pluripotency,由完全的转换字符小狗以及 germline 能干的怪物的产生证实了在啮齿类动物可得到的最紧的测试。有短 telomeres 的转换字符显示出减少的 teratoma 形成和怪物生产,并且没能产生完全的转换字符小狗。Telomere 长度高度被相关(r > 0.8 ) 与转换字符的发展 pluripotency。短 telomeres 减少 proliferative 率或转换字符的能力,改变与 telomere epigenetics 有关的基因的表示,为 embryogenesis 重要的下面调整基因并且破坏细菌房间区别。而且,有更长的 telomeres 的 iPSCs 与短 telomeres 比那些与更高的效率产生怪物。我们功能的 telomeres 为 ESCs/iPSCs 的发展 pluripotency 是必要的并且建议那 telomere 长度可以提供一个珍贵标记评估干细胞 pluripotency 的数据表演,特别地当紧测试不是可行的时。 | Junjiu Huang Fang Wang Maja Okuka Na Liu Guangzhen Ji Xiaoying Ye Bingfeng Zuo Minshu Li Ping Liang William W Ge John CM Tsibris David L Keefe Lin Liu | 2011 | Cell Research2011，21，5： | 3 |
| 17 | Rett syndrome-causing mutations compromise MeCP2- mediated liquid—liquid phase separation of chromatin显示文摘Rett syndrome(RTT),a severe postnatal neurodevelopmental disorder,is caused by mutations in the X-linked gene encoding methyl-CpG-binding protein 2(MeCP2).MeCP2 is a chromatin organizer regulating gene expression.RTT-causing mutations have been shown to affect this function.However,the mechanism by which MeCP2 organizes chromatin is unclear.In this study,we found that MeCP2 can induce compaction and liquid-liquid phase separation of nudeosomal arrays in vitro,and DNA methylation further enhances formation of chromatin condensates by MeCP2.Interestingly,RTT-causing mutations compromise MeCP2-mediated chromatin phase separation,while benign variants have little effect on this process.Moreover,MeCP2 competes with linker histone H1 to form mutually exclusive chromatin condensates in vitro and distinct heterochromatin foci in vivo.RTT-causing mutations reduce or even abolish the ability of MeCP2 to compete with histone H1 and to form chromatin condensates.Together,our results identify a novel mechanism by which phase separation underlies MeCP2-mediated heterochromatin formation and reveal the potential link between this process and the pathology of RTT. | Liang Wang Mingli Hu Mei-Qing Zuo Jicheng Zhao Di Wu Li Huang Yongxin Wen Yunfan Li Ping Chen Xinhua Bao Meng-Qiu Dong Guohong Li Pilong Li | 2020 | Cell Research2020，30，5： | 3 |
| 18 | Three New Coumarins from Micromelum integerrimum显示文摘Three derivatives of micromelin, named hydramicromelins A-C (1-3), were isolated from the aerial part of Micromelum integerrimum (Buch.-Ham) Roem., respectively. Hydramicromelins A-C (1-3) were epimers which possessed the same plane structure and molecular formula. Their structures were elucidated based on MS and NMR data. The relative configurations of 1-3 were established by NOE analysis. | Hong Ping HE,1 Yun ZOU,2 Yue Mao SHEN,1 Xiao Yan HAO,2 Guo Yin ZUO,1 Xiao Jiang HAO1,3* 1Laboratory of Phytochemistry, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204. 2Department of Medicinal Chemistry, Guiyang Medical College, | 2001 | Chinese Chemical Letters2001，12，7： | 3 |
| 19 | Blue-Light-Independent Activity of Arabidopsis Cryptochromes in the Regulation of Steady-State Levels of Protein and mRNA Expression显示文摘Cryptochromes 是调停的蓝光的受体胚轴延伸的蓝光的抑制和在 Arabidopsis 的花的开始的蓝光的刺激。除了他们的 blue-light-dependent 功能, cryptochromes 也涉及生理节奏的钟,展开的子叶,和胚轴抑制的 blue-light-independent 规定。然而,与 cryptochromes 的 blue-light-independent 功能联系的分子的机制仍然保持不清楚。我们这里报导了蛋白质表示的轻规定的比较 proteomics 研究。我们显示出那,期望许多新陈代谢的酶的蛋白质表示响应蓝光和红光变化了。令人惊讶地,一些调整光的蛋白质表示变化在两盏蓝灯和红光在 cry1cry2 异种被损害。这结果建议那,除了调停蛋白质表示的 blue-light-dependent 规定, cryptochromes 也涉及基因表示的 blue-light-independent 规定。与这个假设一致,展出的 cry1cry2 异种响应不仅蓝光,而且红光减少了 mRNA 表示的变化,尽管 red-light-dependent 基因表示变化上的 cryptochrome 效果通常是不太显著的。这些结果支持一个假设除了他们的 blue-light-specific 函数, cryptochromes 也,在基因表示的控制的戏角色由 red/far-red-light 受体 phytochromes 调停了。 | Yue-Jun Yang Ze-Cheng Zuo Xiao-Ying Zhao Xu Li John Klejnot Yan Li Ping Chen Song-Ping Liang Xu-Hong Yu Xuan-Ming Liu Chen-Tao Lin | 2008 | Molecular Plant2008，1，1： | 3 |
| 20 | Effect of steam explosion and microbial fermentation on cellulose and lignin degradation of corn stover显示文摘 | Juan Chang Wei Cheng Qingqiang Yin Ruiyu Zuo Andong Song Qiuhong Zheng Ping Wang Xiao Wang Junxi Liu | 2011 | Bioresource Technology2011，，： | 3 |
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