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| 1 | Glycolysis reprogramming in cancer-associated fibroblasts promotes the growth of oral cancer through the lncRNA H19/miR-675-5p/PFKFB3 signaling pathway显示文摘As an important component of the tumor microenvironment,cancer-associated fibroblasts(CAFs)secrete energy metabolites to supply energy for tumor progression.Abnormal regulation of long noncoding RNAs(lncRNAs)is thought to contribute to glucose metabolism,but the role of lncRNAs in glycolysis in oral CAFs has not been systematically examined.In the present study,by using RNA sequencing and bioinformatics analysis,we analyzed the lncRNA/mRNA profiles of normal fibroblasts(NFs)derived from normal tissues and CAFs derived from patients with oral squamous cell carcinoma(OSCC).LncRNA H19 was identified as a key lncRNA in oral CAFs and was synchronously upregulated in both oral cancer cell lines and CAFs.Using small interfering RNA(siRNA)strategies,we determined that lncRNA H19 knockdown affected proliferation,migration,and glycolysis in oral CAFs.We found that knockdown of lncRNA H19 by siRNA suppressed the MAPK signaling pathway,6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3(PFKFB3)and miR-675-5p.Furthermore,the lncRNA H19/miR-675-5p/PFKFB3 axis was involved in promoting the glycolysis pathway in oral CAFs,as demonstrated by a luciferase reporter system assay and treatment with a miRNA-specific inhibitor.Our study presents a new way to understand glucose metabolism in oral CAFs,theoretically providing a novel biomarker for OSCC molecular diagnosis and a new target for antitumor therapy. | Jin Yang Xueke Shi Miao Yang Jingjing Luo Qinghong Gao Xiangjian Wang Yang Wu Yuan Tian Fanglong Wu Hongmei Zhou | 2021 | International Journal of Oral Science2021，13，1： | 8 |
| 2 | Coprinus comatus-derived nitrogen-containing biocarbon electrocatalyst with the addition of self-generating graphene-like support for superior oxygen reduction reaction显示文摘The development of nitrogen-rich biomassderived carbon catalysts provides an attractive perspective to substitute for Pt-based electrocatalysts for oxygen reduction reaction(ORR). We here report a facile strategy for synthesis of a nitrogen-doped biocarbon/graphene-like composite electrocatalyst by pyrolyzing a solid-state mixture of coprinus comatus biomass and melamine under nitrogen protection. The graphtic carbon nitride formed by polycondensation of melamine at 600 °C acts as a selfsacrificing template to generate the nitrogen-doped graphene-like sheet, which can function as an inserting agent and self-generating support. The composite catalyst exhibits the most promising catalytic activity towards the fourelectron ORR with a half-wave potential of around 0.83 V(vs. RHE), and more excellent stability and tolerance to methanol/ethanol compared to the commercial Pt/C catalyst. It is interestingly found that both a higher content of nitrogen and a larger ratio of graphitic-nitrogen species,which may derive from self-addition of graphene-like support into the catalyst, can effectively improve the electrocatalytic activity. The planar N group may be the nitrogen functionality that is most responsible for maintaining the ORR activity in alkaline medium. This study can largely encourage the exploration of high-performance carbon-based catalysts from economical and sustainable fungus biomass. | Chaozhong Guo Wenli Liao Zhongbin Li Lingtao Sun Haibo Ruan Qingshan Wu Qinghong Luo Jie Huang Changguo Chen | 2016 | Science Bulletin2016，61，12： | 4 |
| 3 | Three-dimensional silk fibroin scaffolds enhance the bone formation and angiogenic differentiation of human amniotic mesenchymal stem cells:a biocompatibility analysis显示文摘Silk fibroin(SF)is a fibrous protein with unique mechanical properties,adjustable biodegradation,and the potential to drive differentiation of mesenchymal stem cells(MSCs)along the osteogenic lineage,making SF a promising scaffold material for bone tissue engineering.In this study,hAMSCs were isolated by enzyme digestion and identified by multiple-lineage differentiation.SF scaffold was fabricated by freeze-drying,and the adhesion and proliferation abilities of hAMSCs on scaffolds were determined.Osteoblast differentiation and angiogenesis of hAMSCs on scaffolds were further evaluated,and histological staining of calvarial defects was performed to examine the cocultured scaffolds.We found that hAMSCs expressed the basic surface markers of MSCs.Collagen type I(COLI)expression was observed on scaffolds cocultured with hAMSCs.The scaffolds potentiated the proliferation of hAMSCs and increased the expression of COL-I in hAMSCs.The scaffolds also enhanced the alkaline phosphatase activity and bone mineralization,and upregulated the expressions of osteogeniorelated factors in vitro.The scaffolds also enhanced the an gioge nic differe ntiati on of hAMSCs.The cocultured scaffolds in creased bone formati on in treating critical calvarial defects in mice.This study first demonstrated that the application of 3D SF scaffolds co-cultured with hAMSCs greatly enhaneed osteogenic differentiation and angiogenesis of hAMSCs in vitro and in vivo.Thus,3D SF scaffolds cocultured with hAMSCs may be a better alter native for bone tissue engin eering. | Yuwan Li Ziming Liu Yaping Tang Qinghong Fan Wei Feng Changqi Luo Guangming Dai Zhen Ge Jun Zhang Gang Zou Yi Liu Ning Hu Wei Huang | 2020 | Acta Biochimica et Biophysica Sinica2020，52，6： | 2 |
| 4 | Fabrication of high-capacity cation-exchangers for protein adsorption: Comparison of grafting-from and grafting-to approaches显示文摘In this work, we have synthesized two polymer-grafted cation exchangers: one via the grafting-from approach, in which sulfopropyl methacrylate (SPM) is grafted through atom transfer radical polymerization onto Sepharose FF (the thus resulting exchanger is referred as Sep-g-SPM), and another via the grafting-to approach, in which the polymer of SPM is directly coupled onto Sepharose FF (the thus resulting exchanger is called as Sep-pSPM). Protein adsorption on these two cation exchangers have been also investigated. At the same ligand density, Sep-g-SPM has a larger accessible pore radius and a smaller depth of polymer layer than Sep-pSPM, due to the controllable introduction of polymer chains with the regular distribution of the ligand. Therefore, high-capacity adsorption of lysozyme and γ-globulin could be achieved simultaneously in Sep-g-SPM with an ionic capacity (IC) of 308 mmol·L^-1. However, Sep-pSPM has an irregular chain distribution and different architecture of polymer layer, which lead to more serious repulsive interaction to proteins, and thus Sep-pSPM has a lower adsorption capacity for γ-globulin than Sep-g-SPM with the similar IC. Moreover, the results from protein uptake experiments indicate that the facilitated transport of adsorbed γ-globulin occurs only in Sep-pSPM and depends on the architecture of polymer layers. Our research provides a clear clue for the development of high-performance protein chromatography. | Ming Zhao Run Liu Jian Luo Yan Sun Qinghong Shi | 2019 | Frontiers of Chemical Science and Engineering2019，13，1： | 1 |
| 5 | Spectrophotometric Method for the Determination of Proteins with Chromotrope 2B 显示文摘 | Liu Shaopu Luo Hongqun | 2001 | Journal of Southwest China Normal University(Nat Sci)2001，26，5： | 1 |
| 6 | Dielectrophoretic Assembly of Carbon Nanotube Chains in Aqueous Solution显示文摘Carbon nanotubes(CNTs)have generated remarkable interests in a wide range of research fields due to their excellent electri-cal properties.However,achieving the CNTs arrangement with high quality in a short time remains a challenge.Herein we studied the in-situ assembly of CNTs based on macroscopic dielectrophoresis by using a centimeter scale electrode,which overcome the limitation of small size in traditional method for manipulating nanoparticles.Ordered CNTs chains could be obtained under the action of alternating current dielectrophoresis by optimizing the voltage and frequency.Besides,the ordered chains were able to restore immediately upon powering up after being damaged.Furthermore,a CNTs chain was prepared for conducting the wet circuit and powering a LED,and different conductive patterns on the non-woven fabric were achieved by controlling the position of the electrodes in wet environment. | Dan Zhao Rui Liu Cheng Luo Yang Guo Chengyi Hou Qinghong Zhang Yaogang Li Wei Jia Hongzhi Wang | 2021 | Advanced Fiber Materials2021，3，5： | 1 |
| 7 | The Effect of Martian Ionospheric Dispersion on SAR Imaging显示文摘When passing through the ionosphere,the high-frequency(HF)pulse signal of the Mars Exploration Radar is affected by the dispersion effect error,which results in signal attenuation and time delay and brings about a phase advance in such a way that the echo cannot be matched and filtered.In this paper,a high-order phase model is built to overcome the above problems and enable echo matching and filtering.Most studies on the dispersion effect approximate the additional phase after the effect,assuming that the ionosphere is a thin-layer structure.In this paper,an effective model for the HF waveband is constructed to analyze the change of signal propagation paths in the ionosphere.The additional phase is expanded in a Taylor series and retained these expansions as high-order terms to calculate the cumulative additional phase along the path.We show the rangeoffset variables of signal frequency,bandwidth,and electron density,simulate the effects of the ionosphere under different conditions,and conclude that the model can effectively estimate Mars without considering the effects of magnetic fields and anomalous solar activity and the effect of the ionosphere on synthetic aperture radar(SAR)echoes.The results obtained using ray tracing calculations are different from those obtained by simplifying assumptions,and we can simulate the Martian ionospheric effects by the former. | Bo Wang Xijin Luo Qinghong Sheng Zhijun Yan | 2022 | Space(Science & Technology)2022，，1： | 0 |
| 8 | A self-powered ultraviolet photodetector with van der Waals Schottky junction based on TiO_(2) nanorod arrays/Au-modulated V2CTx MXene显示文摘A self-powered ultraviolet photodetector(UV PD)with van der Waals(vdW)Schottky junction based on TiO_(2) nanorod arrays/Au-modulated V2 CTx MXene is reported.The Schottky junction enables the device to operate in self-powered mode.The dangling bond-free surface of V2 CTx MXene reduces the charge recombination at the junction interface.Meanwhile,V2 CTx MXene,with the work function(WF)increasing to 5.35 eV,forms a hole transport layer by contacting with Au electrode,which facilitates the carrier extraction.The electron lifetime in the device has prolonged to 8.95μs.As a result,the responsivity and detectivity of the PD have achieved 28 mA/W and 1.2×10^(11) cm Hz1/2/W(340 nm,65 mW/cm2,0 V),respectively.In addition,the presence of the Au electrode prevents the vanadium from coming into contact with oxygen and oxidizing,preserving the properties of the V2 CTx films.After 180 days of exposure to the atmosphere,the device performance remained at a particularly high level,indicating enhanced durability.This work points out an effective approach to modulate the properties of V2 CTx to obtain the high performance and stability of the UV PD. | Guangcan Luo Ziling Zhang Yabing Wang Qun Deng Shengtao Pan Tengfei Wang Qinghong Li Kaixiang Liu Pengfei Kong Jing Zhang Shengyun Luo Hong Lin | 2023 | Journal of Materials Science & Technology2023，，25： | 0 |
| 9 | Adsorption equilibrium of binary proteins to Q Sepharose FF显示文摘Simultaneous and sequential adsorptions of bovine serum albumin(BSA) and bovine hemoglobin(bHb) was investigated on anion ion-exchange media Q Sepharose FF in batch adsorption experiments to elucidate the mechanism of multi-component protein adsorption.BSA is always preferential adsorbed with bHb inhibited in binary adsorption.Influence on binary adsorption of factors such as pH,salt concentration,initial concentration ratios of proteins and the adsorption sequence are determined.Three models have been tested and compared to describe the competitive binary adsorption of BSA and bHb in all conditions studied.Among the extended Langmuir model gives the best predict of adsorption of the stronger adsorbed protein when its adsorption is not very strong in simultaneous adsorption,the Statistical thermodynamic(ST) model gives the best description of weaker adsorbed protein in simultaneous adsorption,the Steric mass-action(SMA) model is most appropriate multi-component model considering all different conditions for both proteins. | LIANG Juan~(1,2),FIEG Georg~2,LUO Xing~2,SHI Qinghong~1,SUN Yan~1 (1 Department of Biochemical Engineering and Key Laboratory of Systems Bioengineering of Ministry of Education, School of Chemical Engineering and Technology,Tianjin University,Tianjin 300072,China 2 Institute of Process and Plant Engineering,Hamburg University of Technology,Schwarzenbergstrasse 95,21073 Hamburg,Germany) | 2011 | 化工进展2011，30，S2： | 0 |
| 10 | Activated mouse CD4+Foxp3- T cells facilitate melanoma metastasis via Qa-1-dependent suppression of NK-cell cytotoxicity显示文摘 | Xiaojuan Wang Yanyan Cui Gaoxmg Luo Qinghong Wang Jie Hu Weifeng He Jun Yuan Junyi Zhou Yan Wu Xiaofeng Sun Simon C Robson Xianchang Li Jiangling Tan Yanmeng Peng Gang Xue Linrong Lu Wenda Gao Jun Wu | 2012 | Cell Research2012，22，12： | 0 |
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