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| 1 | Transcriptomic Response to Yersinia pestis:RIG-I Like Receptor Signaling Response Is Detrimental to the Host against Plague显示文摘Bacterial pathogens have evolved various mechanisms to modulate host immune responses for successful infection.In this study,RNAsequencing technology was used to analyze the responses of human monocytes THP1 to Yersinia pestis infection.Over 6000 genes were differentially expressed over the 12 h infection.Kinetic responses of pathogen recognition receptor signaling pathways,apoptosis,antigen processing,and presentation pathway and coagulation system were analyzed in detail.Among them,RIG-I-like receptor(RLR) signaling pathway,which was established for antiviral defense,was significantly affected.Mice lacking MAVS,the adaptor of the RLR signahng pathway,were less sensitive to infection and exhibited lower IFN-P production,higher Thl-type cytokines IFN-y and IL-12 production,and lower Th2-type cytokines EL-4 and IL-13 production in the serum compared with wild-type mice.Moreover,infection of pathogenic bacteria other than Y.pestis also altered the expression of the RLR pathway,suggesting that the response of RLR pathway to bacterial infection is a universal mechanism. | Zongmin Du Huiying Yang Yafang Tan Guang Tian Qingwen Zhang Yujun Cui Yanfeng Yan Xiaohong Wu Zuyun Chen Shiyang Cao Yujing Bi Yanping Han Xiaoyi Wang Yajun Song Ruifu Yan | 2014 | Journal of Genetics and Genomics2014，41，7： | 2 |
| 2 | Tackling COVID-19:Insights from the Qinghai Province plague prevention and control(PPC)model显示文摘Plague,caused by Yersinia pestis,is a natural focus infectious disease.In China,plague is classified as category A,with the highest risk and hazard among the infectious diseases.Qinghai used to be considered as one of the most serious areas of plague in China.In recent years,thank to the measures in eight aspects summarized as the“Qinghai model”which were adopted to prevent and control the human plague in Qinghai,Qinghai has not experienced any plague case reported for eight years.In early 2020,coronavirus disease 2019(COVID-19)outbroke in China.The Qinghai model on plague was employed to deal with the COVID-19 emergency in Qinghai Province.The Qinghai Center for Disease Control and Prevention(Qinghai CDC)and hospitals,along with the departments of public security,animal husbandry and other departments,quickly tracked and treated the patients with severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)and started surveillance programs on close contacts timely.At present,the cure rate of patients has reached 100%,and close contacts have been effectively quarantined and tested to avoid the spread of COVID-19.The findings from the study suggest that the prevention and control measures undertaken in Qinghai Province might be effective in dealing with the category A infectious diseases such as COVID-19 and other diseases. | Haisheng Wu Qingwen Zhang Hailian Wu Fuzhang Tian Baizhong Cui Zhizhen Qi Xiaoqing Xu Xuefei Zhang HuWang | 2020 | Biosafety and Health2020，2，4： | 1 |
| 3 | Synthesis of carbon dots with a tunable photoluminescence and their applications for the detection of acetone and hydrogen peroxide显示文摘Carbon dots(CDs) with multi-color emissive properties and a high photoluminescent quantum yield(PLQY) have attracted great attention recently due to their potential applications in chemical,environmental,biological and photo-electronic fields.Solvent-dependent effect in photoluminescence provides a facial and effective approach to tune the emission of CDs.In this study,green emissive nitrogen-doped carbon dots(N-CDs) are synthesized from p-hydroquinone and ethylenediamine through a simple hydrothermal method.The as-prepared N-CDs possess a robust excitation-independent green luminescence and a high PLQY of up to 15.9%.Further spectroscopic characterization indicates that the high PLQY is achieved by the balance of nitrogen doping states and the surface passivation extent in CDs.The N-CDs also exhibit solvent-dependent multi-color emissive property and distinct PLQY in different solvents(the maximum can reach up to 25.3%).Furthermore,the as-prepared N-CDs are applied as fluorescence probes to detect acetone and H2O2 in water.This method has exhibited a low detection limit of acetone(less than 0.1 %) and a quick and linear response to the H_2O_2 with the concentration from 0 to 120 μmol/L.This work broadens the knowledge of applying CDs as probes in the bio and chemical sensing fields. | Shitong Cui Yanfen Wu Yao Liu Qingwen Guan Yida Zhang Yilin Zhang Song Luo Meng Xu Juncheng Wang | 2020 | Chinese Chemical Letters2020，31，2： | 1 |
| 4 | Dual-Ion Co-Regulation System Enabling High-Performance Electrochemical Artificial Yarn Muscles with Energy-Free Catch States显示文摘Artificial yarn muscles show great potential in applications requiring low-energy consumption while maintaining high performance. However, conventional designs have been limited by weak ion-yarn muscle interactions and inefficient “rocking-chair” ion migration. To address these limitations, we present an electrochemical artificial yarn muscle design driven by a dual-ion co-regulation system. By utilizing two reaction channels, this system shortens ion migration pathways, leading to faster and more efficient actuation. During the charging/discharging process, PF_6~- ions react with carbon nanotube yarn, while Li~+ ions react with an Al foil. The intercalation reaction between PF_6~- and collapsed carbon nanotubes allows the yarn muscle to achieve an energy-free high-tension catch state. The dual-ion coordinated yarn muscles exhibit superior contractile stroke, maximum contractile rate, and maximum power densities, exceeding those of “rocking-chair” type ion migration yarn muscles. The dual-ion co-regulation system enhances the ion migration rate during actuation, resulting in improved performance. Moreover, the yarn muscles can withstand high levels of isometric stress, displaying a stress of 61 times that of skeletal muscles and 8 times that of “rocking-chair” type yarn muscles at higher frequencies. This technology holds significant potential for various applications, including prosthetics and robotics. | Ming Ren Lizhong Dong Xiaobo Wang Yuxin Li Yueran Zhao Bo Cui Guang Yang Wei Li Xiaojie Yuan Tao Zhou Panpan Xu Xiaona Wang Jiangtao Di Qingwen Li | 2023 | Nano-Micro Letters2023，15，10： | 1 |
| 5 | Phosphorylation of OsRbohB by the protein kinase OsDMI3 promotes H_(2)O_(2) production to potentiate ABA responses in rice显示文摘In rice, the Ca^(2+)/calmodulin-dependent protein kinase OsDMI3 is an important positive regulator of abscisic acid (ABA) signaling. In ABA signaling, H_(2)O_(2) is required for ABA-induced activation of OsDMI3, which in turn increase H_(2)O_(2) production. However, how OsDMI3 regulates H_(2)O_(2) production in ABA signaling remains unknown. Here we show that OsRbohB is the main NADPH oxidase involved in ABA-induced H_(2)O_(2) production and ABA-mediated physiological responses. OsDMI3 directly interacts with and phosphorylates OsRbohB at Ser-191, which is OsDMI3-mediated site-specific phosphorylation in ABA signaling. Further analyses revealed that OsDMI3-mediated OsRbohB Ser-191 phosphorylation positively regulates the activity of NADPH oxidase and the production of H_(2)O_(2) in ABA signaling, thereby enhancing the sensitivity of seed germination and root growth to ABA and plant tolerance to water stress and oxidative stress. Moreover, we discovered that the OsDMI3-mediated OsRbohB phosphorylation and H_(2)O_(2) production is dependent on the sucrose non-fermenting 1-related protein kinases SAPK8/9/10, which phosphorylate OsRbohB at Ser-140 in ABA signaling. Taken together, these results not only reveal an important regulatory mechanism that directly activates Rboh for ABA-induced H_(2)O_(2) production but also uncover the importance of this regulatory mechanism in ABA signaling. | Qingwen Wang Tao Shen Lan Ni Chao Chen Jingjing Jiang Zhenzhen Cui Shuang Wang Fengjuan Xu Runjiao Yan Mingyi Jiang | 2023 | Molecular Plant2023，16，5： | 0 |
| 6 | The NADPH oxidase OsRbohA increases salt tolerance by modulating K^(+)homeostasis in rice显示文摘Rice(Oryza sativa L.)is a staple cereal for more than two thirds of the world's population.Soil salinity severely limits rice growth,development,and grain yield.It is desirable to elucidate the mechanism of rice's salt-stress response.As the major source of H_(2)O_(2),NADPH oxidase(Rboh)is believed to be involved in salt-stress tolerance.However,the function and mechanism of rice Rboh in salt stress response remain unclear.In this study,we found that the expression of OsRbohA was up-regulated by NaCl treatment in the shoots and roots of rice seedlings.Knockout of OsRbohA reduced the tolerance of rice to salt stress.Knockout of OsRbohA blocked NaCl-induced increases of NADPH activity and H_(2)O_(2) content in roots.OsRboh A knockout inhibited root growth and disrupted K^(+)homeostasis by reducing the expression of K^(+) transporters and channel-associated genes(OsGORK,OsAKT1,OsHAK1,and OsHAK5)in roots under NaCl treatment.Under NaCl treatment,OsRbohA knockout also reduced subcellular K^(+) contents of the plasma membrane and soluble fraction.Overexpression of OsRbohA increased the expression of K^(+) transporters and channel-associated genes and reduced the loss of K^(+) ions in roots.These results indicate that OsRboh A-mediated H_(2)O_(2) accumulation modulates K^(+) homeostasis,thereby increasing salt tolerance in rice. | Qingwen Wang Lan Ni Zhenzhen Cui Jingjing Jiang Chao Chen Mingyi Jiang | 2022 | The Crop Journal2022，10，6： | 0 |
| 7 | OsDMI3-mediated OsUXS3 phosphorylation improves oxidative stress tolerance by modulating OsCATB protein abundance in rice显示文摘Calcium(Ca^(2+))/calmodulin(CaM)-dependent protein kinase(CCaMK)is an important positive regulator of antioxidant defenses and tolerance against oxidative stress.However,the underlying molecular mechanisms are largely unknown.Here,we report that the rice(Oryza sativa)CCa MK(OsDMI3)physically interacts with and phosphorylates OsUXS3,a cytosol-localized UDP-xylose synthase.Genetic and biochemical evidence demonstrated that OsUXS3 acts downstream of OsDMI3 to enhance the oxidative stress tolerance conferred by higher catalase(CAT)activity.Indeed,OsUXS3 interacted with CAT isozyme B(OsCATB),and this interaction was required to increase OsCATB protein abundance under oxidative stress conditions.Furthermore,we showed that OsDMI3 phosphorylates OsUXS3 on residue Ser-245,thereby further promoting the interaction between OsUXS3 and OsCATB.Our results indicate that OsDMI3 promotes the association of OsUXS3 with OsCATB to enhance CAT activity under oxidative stress.These findings reveal OsUXS3 as a direct target of OsDMI3 and demonstrate its involvement in antioxidant defense. | Lan Ni Qingwen Wang Chao Chen Shuang Wang Tao Shen Jingjing Jiang Zhenzhen Cui Kaiyue Li Qiqing Yang Mingyi Jiang | 2022 | Journal of Integrative Plant Biology2022，64，5： | 0 |
| 8 | Dual-signal readout paper-based wearable biosensor with a 3D origami structure for multiplexed analyte detection in sweat显示文摘In this research,we design and implement a small,convenient,and noninvasive paper-based microfluidic sweat sensor that can simultaneously detect multiple key biomarkers in human sweat.The origami structure of the chip includes colorimetric and electrochemical sensing regions.Different colorimetric sensing regions are modified with specific chromogenic reagents to selectively identify glucose,lactate,uric acid,and magnesium ions in sweat,as well as the pH value.The regions of electrochemical sensing detect cortisol in sweat by molecular imprinting.The entire chip is composed of hydrophilically and hydrophobically treated filter paper,and 3D microfluidic channels are constructed by using folding paper.The thread-based channels formed after the hydrophilic and hydrophobic modifications are used to control the rate of sweat flow,which in turn can be used to control the sequence of reactions in the differently developing colored regions to ensure that signals of the best color can be captured simultaneously by the colorimetric sensing regions.Finally,the results of on-body experiments verify the reliability of the proposed sweat sensor and its potential for the noninvasive identification of a variety of sweat biomarkers. | Yuemeng Cheng Shaoqing Feng Qihong Ning Tangan Li Hao Xu Qingwen Sun Daxiang Cui Kan Wang | 2023 | Microsystems & Nanoengineering2023，9，2： | 0 |
| 9 | Ecological Anthropological Research on the Vertical Agriculture of Lisu People: A Case Study of Tongle Village in Weixi County of Yunnan Province显示文摘Tongle Village in Weixi County of Yunnan Province,a typical Lisu village in Lancang River basin,is located at the national protection zone of traditional culture of Lisu people and named as the ecological cultural village by UNESCO. Based on the distinctive climatic characteristics at different altitudes,Lisu people have developed an effective vertical agriculture system. They cultivate rice and wheat in the river basin at an altitude of 1 740 m; plant corns,nuts,fruits and vegetables around the village at an altitude of 1 840m and buckwheat,potato, herbs in the upper ' swidden land' at an altitude of 2 000 m. They also herd animals in the alpine meadow at an altitude of 2 500 m. Based on a fieldwork,this paper studied the vertical agriculture system by applying the theories of Ecological Anthropology and employing several field work methods such as participant observation and in-depth interviews. According to the research findings,Lisu people have not only properly utilized local natural resources,but also developed a related cultural system to facilitate their agriculture production. But the vertical system also faces great challenges with the economic and social development of local areas. To respond positively,sustainable development is a way for Lisu people to enhance their living standards and maintain their traditional culture. | Hanbai HAN Mingkun CUI Qingwen MIN | 2013 | Asian Agricultural Research2013，5，6： | 0 |
| 10 | Finger-actuated wireless-charging wearable multifunctional sweatsensing system for levodopa and vitamin C显示文摘Efficient portable wearable sweat sensors allow the long-term monitoring of changes in the status of biomarkers in sweat,which can be useful in diagnosis,medication,and nutritional assessment.In this study,we designed and tested a wireless,battery-free,flexible,self-pumping sweat-sensing system that simultaneously tracks levodopa and vitamin C levels in human sweat and detects body temperature.The system includes a microfluidic chip with a self-driven pump and anti-reflux valve,a flexible wireless circuit board,and a purpose-designed smartphone app.The microfluidic chip is used for the efficient collection of sweat and the drainage of excess sweat.The dual electrochemical sensing electrodes in the chip are modified with functional materials and appropriate enzymatic reagents,achieving excellent selectivity and stability.The sensitivities of the levodopa sensor and the vitamin C sensor are 0.0073 and 0.0018μA·μM^(-1),respectively,and the detection correlation coefficients of both exceed 0.99.Both sensors have a wide linear detection range of 0–100 and 0–1000μM,respectively,and low detection limits of 0.28 and 17.9μM,respectively.The flexible wireless circuit board is equipped with the functions of wireless charging,electrical signal capture and processing,and wireless transmission.The data recorded from each sensor are displayed on a smartphone via a self-developed app.A series of experimental results confirmed the reliability of the sweat-sensing system in noninvasively monitoring important biomarkers in the human body and its potential utility in the comprehensive assessment of biological health. | Qihong Ning Shaoqing Feng Qingwen Sun Ruoyao Yu Tangan Li Hao Xu Daxiang Cui Kan Wang | 2024 | Nano Research2024，17，4： | 0 |
| 11 | High‑Temperature‑Tolerant Artificial Muscles Using Poly(p‑phenylene benzobisoxazole)Composite Yarns显示文摘Today the developed yarn muscles or actuators still cannot satisfy the requirements of working in high-temperature environ-ments.Thermal resistivity is highly needed in aerospace and industrial protection applications.Herein,an artificial muscle with high-temperature tolerance is prepared using carbon nanotube(CNT)wrapped poly(p-phenylene benzobisoxazole)(PBO)composite yarns.A thermal twisting method was utilized to reorientate the stiff PBO molecular chains into a uniform and twist-stable coiled structure.The CNT/PBO composite yarn muscle generates reversible contractile strokes up to 18.9%under 5.4 MPa tension and outputs 1.3 kJ kg^(-1) energy density.In contrast to previous actuators,which are normally oper-ated at room temperatures,the CNT/PBO composite yarn muscles can work at ambient temperatures up to 300℃ with high contractile stroke and long-term stability.A bionic inchworm robot,a deployable structure,and smart textiles driven by the high-temperature-tolerant yarn muscles were demonstrated,showing the promise as a soft actuator towards high-temperature environment applications. | Jianfeng He Ming Ren Lizhong Dong Yulian Wang Xulin Wei Bo Cui Yulong Wu Yueran Zhao Jiangtao Di Qingwen Li | 2022 | Advanced Fiber Materials2022，4，5： | 0 |
| 12 | A recombineering system for Bacillus subtilis based on the native phage recombinase pair YqaJ/YqaK显示文摘Bacillus subtilis plays an important role in fundamental and applied research,and it has been widely used as a cell factory for the production of enzymes,antimicrobial materials,and chemicals for agriculture,medicine,and industry.However,genetic manipulation tools for B.subtilis have low efficiency.In this work,our goal was to develop a simple recombineering system for B.subtilis.We showed that genome editing can be achieved in B.subtiliis 1A751 through co-expression of YqaJ/YqaK,a native phage recombinase pair found in B.sub-tilis 168,and the competence master regulator ComK using a double-stranded DNA substrate with short ho-mology arms(100 bp)and a phosphorothioate modification at the 5′-end.Efficient gene knockouts and large DNA insertions were achieved using this new recombineering system in B.subtilis 1A751.As far as we know,this is the first recombineering system using the native phage recombinase pair YqaJ/YqaK in B.subtilis.In conclusion,this new recombineering system provides a simple and fast tool for genetic manipulation of B.sub-tilis,and it will promote studies of genome function,construction of production strains,and genome mining in B.subtilis. | Qingshu Liu Ruijuan Li Hongbo Shi Runyu Yang Qiyao Shen Qingwen Cui Xiuling Wang Aiying Li Youming Zhang Jun Fu | 2023 | Engineering Microbiology2023，3，3： | 0 |
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